Cloning a gene into a plasmid often requires you to amplify the target DNA sequence, then digest and paste the target sequence into the plasmid. Several purified enzymes are important in this process. Which of the following enzymes would act LAST in the cloning process?

W) Polymerase [POL-uh-muh-rays]

X) Ligase [LY-gays]

Y) Exonuclease [eks-uh-NOO-klee-ays]

Z) Restriction endonuclease [en-duh-NOO-klee-ays]
X) LIGASE