HUH-tag
HUH endonucleases (HUH-tags) are sequence-specific single-stranded DNA (ssDNA) binding proteins originating from numerous species of bacteria and viruses. Viral HUH endonucleases are involved in initiating rolling circle replication while ones of bacterial origin initiate bacterial conjugation. In biotechnology, they can be used to create protein-DNA linkages, akin to other methods such as SNAP-tag. In doing so, they create a 5' covalent bond between the ssDNA and the protein. HUH endonucleases can be fused with other proteins or used as protein tags.
Q415601
endonuclease
Concept
Q7430
DNA
Concept
Q2252764
binding proteins
Concept
Q3112822
rolling circle replication
UNK
Q273193
bacterial conjugation
Concept
Q2295756
SNAP-tag
UNK
Q127920
covalent bond
Concept
Q425158
fused
Concept
Q645590
protein tag
Concept
Q97358848
HUH-tag
MISC
+5^^http://www.w3.org/2001/XMLSchema#decimal
5
NUMBER
subclass of