text: what was the impact of pastuer disproving spontaneous generation?   redi and spallanzani had already done some experiments disproving the hypothesis of spontaneous generation. however, many people still believed in this false idea.they argued that air was necessary for spontaneous generation. spallanzani had kept air out of his experiment.  when pasteur did his experiment, he did not seal the end of his flask, the end of the flask was open to the air but had a long, curved neck. he showed that microorganisms did not arise in a boiled nutrient broth even if it is exposed to air as long as airborne matter was not allowed to enter the broth.  louis pasteur put an end to to the spontaneous generation hypothesis.he definitely proved that life comes only from life.