text: can frozen sperm work again if defrosted? can it clone animals? too hard sperm only has one set of chromosomes. it must combine with another set such as in an ova, egg.  i believe artificial insemination of farm animals is done with frozen sperm -425f with liquid nitrogen (-273c, 70k). human sperm banks as well.  i hope it is defrosted before inserted into a cow. that could hurt to have a frozen...