text: why alkaline buffer is mostly used in protein separation? state its importance? role of alkaline buffer in protein separation the usage of an alkaline buffer in a low ph matrix of proteins causes the proteins to migrate to collection nodes in a process called "electrophoresis".   electrophoresis is the process by which more highly charged molecular structures are moved through an electrical field and leave the lesser charged elements behind.      in separation processes using polyacrylamide gel, which is the most common matrix and used to separate relatively larger proteins.  the addition of a mildly alkaline ph leaves most of the proteins anionic and causes them to migrate to ionic nodes (much like the allergenic air filters)       in summery alkaline ph substances are used to make proteins anionic so they can be separated using an ionic node.      (thanks tho finding out stuff about this one was fun ^_^, and good luck)