text: what is the best way to extract genomic dna out of staphyloccocus bacteria? ive tried with a dna mini prep kit using lysozyme, but it didn't work, the kit suggests using lysostaphin, but its expensive, anyother suggestions? when i was working in the biochemistry lab, i was using sonication first to lyse the bacteria.  i don't know for certain about your case, but the lysozyme wasn't strong enough for for my bacteria.  i used lysozymes for the larger, less durable mammalian cells.