text: western blotting for jnk and p jnk using hela/pa-1 cells protocol?   i have used cell signaling technologies antibodies for jnk, and if you check their website, they have a nice protocol that works in many cell types.  i typically run 10% sds-page gels for jnk, and be sure to include pmsf, protease inhibitors and naovo3 in your lysis buffer-- and keep your lysates on ice at all times.