text: how to characterise recombinant proteins?   hmm, not sure what you're looking for. a recombinant protein is characterised by amino-acid sequencing. this gives the primary structure. futher analysis of secondary and tertiary structure can be made using x-ray diffraction and nmr. predicitions of structure are made using computer programs which analyse the amino acid sequence and compare it with known structures, they then extrapolate this to impose an expected structure on the protein.