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Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points (2018\).

*Authors: Li\-Hui Li,1,2Qiang\-Min Huang,1 Marco Barbero,2 Lin Liu,3 Thi\-Tham
Nguyen,4 Matteo Beretta\-Piccoli,2 An\-Le Xu,1 Li\-Juan Ji1*
**ABSTRACT**
**Background:** Proteomics analysis may provide important information regarding
the pathogenesis of chronic myofascial pain and the mechanisms underlying the
treatment effects of dry needling. **Materials and methods:**This study used
a rat model of myofascial trigger points (MTrPs) to perform a proteomics
analysis. Three biological replicate experiments were used to compare the
proteomes of healthy control rats, a rat model of MTrP, MTrP model rats
following dry needling of MTrPs, and MTrP model rats following dry needling of
non\-MTrPs. Tandem mass tag (TMT) labeling technology based on nanoscale liquid
chromatography\-tandem mass spectrometry was used. Hierarchical clustering, gene
ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis,
and protein–protein interaction network analysis were performed to characterize
the proteins. To validate the TMT results, three candidate biomarker proteins
were verified using parallel reaction monitoring and Western blot analysis. 
**Results:**A total of 2,635 proteins were identified. GO and KEGG enrichment
analyses showed that the glycolysis/gluconeogenesis pathways played dominant
roles in the pathogenesis of chronic myofascial pain. The three candidate
biomarker proteins were the pyruvate kinase muscle isozyme (encoded by
the *PKM* gene), the muscle isoform of glycogen phosphorylase (encoded by
the *PYGM*gene), and myozenin 2 (encoded by the *MYOZ2* gene). The validation
results were consistent with the TMT results. **Conclusion:** This is the
first proteomics study that has investigated the pathogenesis of chronic
myofascial pain and the mechanisms underlying the treatment effects of dry
needling in an in vivo rat model of MTrPs, which might promote our understanding
of the molecular mechanisms underlying chronic myofascial pain.
**Keywords:** musculoskeletal pain, acupuncture, bioinformatics, mass
spectrometry, tandem mass tag, parallel reaction monitoring
* Link bài viết: *[Tham khảo thêm tại đây]( https://doi.org/10.2147/JPR.S185916)*

Structural and functional abnormalities of motor endplates in rat skeletal model of myofascial trigger spots (2019\).

*Authors: Qing\-GuangLiua , Qiang\-MinHuangb, LinLiuc, Thi\-ThamNguyend*
**ABSTRACT**
Myofascial trigger points (MTrPs) are defined as hyperirritable spots in a
palpable taut band (TB) of skeletal muscle fibers. Knowing the formation and
location of MTrPs is a great help to prevent their development and inactivate
existing MTrPs. This study aimed to obtain new evidence that myofascial trigger
spots (MTrSs), which are similar to human MTrPs, are found in dysfunctional
motor endplates by observing the morphological characteristics of muscles and
changes in biochemical substances. A total of 32 male Sprague Dawley rats were
randomly divided into four groups: two control groups (i.e., C1 and C2\) and two
model groups (i.e., M1 and M2\). C1 and M1 were used for acetylcholine (ACh)
content measurement, while C2 and M2 were utilized for acetylcholinesterase
(AChE) staining. In the model groups, blunt striking injury was induced and
eccentric exercise was applied to the left gastrocnemius for 8 weeks. After 1
month, spontaneous electrical activity(SEA), AChE optical density (OD), muscle
fiber diameter, and ACh content were measured. The results showed that extensive
abnormal endplate noise (aEPN), including positive neurons, fibrillation
potentials, fasciculation potential, and high amplitude (endplate spikes
\[EPS]), is present at MTrSs in M1\. Quantitative electromyography results
showed that the amplitudes of aEPN and frequency of EPS in M1 were significantly
higher than those of C1\. The ACh content of MTrSs in M1 was significantly
higher than that in C1\. The AChE OD value of M2 was significantly lower than
that of C2\. In addition, the diameter of the muscle fibers in the AChE\-stained
area was longer in M2 than in C2\. In conclusion, MTrSs formed at the motor
endplate with a larger diameter of muscle fibers. Excessive ACh release and
decreased AChE activity at MTrSs stimulated muscle action potential and muscle
contraction.
* Link bài viết: *[Tham khảo thêm tại đây](https://doi.org/10.1016/j.neulet.2019.134417)*

Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study (2019\).

*Authors: Lihui Li1,2, Qiangmin Huang1, Marco Barbero2, Lin Liu3, Thitham
Nguyen4, Anle Xu1,and Lijuan Ji1*
**ABSTRACT**
A quantitative proteomic analysis of the response to dry needling combined with
static stretching treatment was performed in a rat model of active myofascial
trigger points (MTrPs). 36 rats were divided into a model group (MG), a
stretching group (SG) and a dry needling combined with stretching group (SDG).
We performed three biological replicates to compare large\-scale differential
protein expression between groups by tandem mass tag (TMT) labeling technology
based on nanoscale liquid chromatography mass spectrometry analysis (LC–MS/MS).
Hierarchical clustering, Gene Ontology (GO), Kyoto Encyclopedia of Genes and
Genomes (KEGG) enrichment and protein\-protein interaction network analyses were
performed for the general characterization of overall enriched proteins. For
validation of the results of TMT, the candidate proteins were verified by
parallel reaction monitoring (PRM) analysis. 285 differentially expressed
proteins between groups were identified and quantified. Tight junction pathway
played a dominant role in dry needling combined with static stretching treatment
for the rat model of active MTrPs. Three candidate proteins, namely actinin
alpha 3, calsequestrin\-1 and parvalbumin alpha, were further validated,
consistent with the results of LC–MS/MS. This is the first proteomics\-based
study to report the therapeutic mechanism underlying dry needling and static
stretching treatment for MTrPs. Further functional verification of the potential
signaling pathways and the enriched proteins is warranted. View Full\-Text
***Keywords:** myofascial trigger points; proteomic; chronic myofascial pain;
dry needling; stretching; mass spectrometry; tandem mass tag; actinin alpha 3;
calsequestrin\-1; parvalbumin alpha*
* Link bài viết: [*Tham khảo thêm tại đây*](https://doi.org/10.3390/ijms20030564)

Relationship between muscle spindles and myofascial trigger spots according to Hoffmann reflex pathway and tissue morphology characteristics in a rat model (2020\).

*Authors: Lin Liu1,  Qiang\-Min Huang2,3, Qing\-Guang Liu4, Thi\-Tham Nguyen5,
Jian\-Qin Yan6, Cheng\-Zhi Bo1.*
**ABSTRACT**
**Objectives:**To determine how muscle spindles are involved in the
pathophysiology of chronic myofascial trigger spots (MTrSs, similar to
myofascial trigger points) in a rat injury model according to the
characteristics of the Hoffmann reflex (H\-reflex) and the anatomical
relationship between muscle spindles and MTrSs.
**Methods:** 16 male Sprague\-Dawley rats (7 weeks old) were randomly divided
into experimental and control groups. A blunt strike injury and eccentric
exercise were applied to the gastrocnemius muscle of rats in the experimental
group once a week for 8 weeks as a MTrS modelling intervention. Subsequently,
the rats were reared normally and rested for 4 weeks. At the end of the 12th
week, the rats were examined for the presence of MTrSs defined by the detection
of a palpable taut band exhibiting both a local twitch response and spontaneous
electrical activity. After modelling, evocation of the H\-reflex and
morphological examination of muscle spindles and MTrSs were conducted.
**Results:** The threshold (0\.35±0\.04 mA) of the H\-reflex and latency
(1\.24±0\.18 ms) of the M wave recorded at MTrSs were not significantly
different to those at non\-MTrSs (P\>0\.05\). Compared with non\-MTrSs, a lower
Mmax (4\.28±1\.27 mV), higher Hmax (median (IQR) 0\.95 (0\.80–1\.08\) mV) and
Hmax/Mmax (median (IQR) 0\.21 (0\.16–0\.40\)), and shorter H wave latency
(4\.60±0\.89 ms) were recorded at MTrSs (P\<0\.05\). Morphologically, there was
a close anatomical relationship between the MTrS cells and the muscle spindles.
**Discussion:**Compared with normal muscles, the H\-reflex myoelectrical
activity was enhanced and some muscle spindles might have been influenced by
active MTrSs. Thus, muscle spindles may play an important role in the
pathological mechanism underlying myofascial trigger points.
* Link bài viết: [*Tham khảo thêm tại đây*]( https://doi.org/10.1136/acupmed-2017-011626)
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Công trình công bố ngoài nước | Khoa Khoa học thể thao Nhảy đến nội dung Đại học Tôn Đức Thắng Mon - Fri : 09:00 - 17:00 fss@tdtu.edu.vn Khoa Khoa học thể thao Main navigation Vi Trang chủ Giới thiệu Đào tạo Nghiên cứu Tuyển sinh CLB - Đội nhóm Search Công trình công bố ngoài nước Breadcrumb FSS  - Công trình công bố ngoài nước Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points (2018). Authors: Li-Hui Li, 1,2 Qiang-Min Huang, 1 Marco Barbero, 2 Lin Liu, 3 Thi-Tham Nguyen, 4 Matteo Beretta-Piccoli, 2 An-Le Xu, 1 Li-Juan Ji 1  ABSTRACT  Background: Proteomics analysis may provide important information regarding the pathogenesis of chronic myofascial pain and the mechanisms underlying the treatment effects of dry needling. Materials and methods: This study used a rat model of myofascial trigger points (MTrPs) to perform a proteomics analysis. Three biological replicate experiments were used to compare the proteomes of healthy control rats, a rat model of MTrP, MTrP model rats following dry needling of MTrPs, and MTrP model rats following dry needling of non-MTrPs. Tandem mass tag (TMT) labeling technology based on nanoscale liquid chromatography-tandem mass spectrometry was used. Hierarchical clustering, gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and protein–protein interaction network analysis were performed to characterize the proteins. To validate the TMT results, three candidate biomarker proteins were verified using parallel reaction monitoring and Western blot analysis. Results: A total of 2,635 proteins were identified. GO and KEGG enrichment analyses showed that the glycolysis/gluconeogenesis pathways played dominant roles in the pathogenesis of chronic myofascial pain. The three candidate biomarker proteins were the pyruvate kinase muscle isozyme (encoded by the PKM gene), the muscle isoform of glycogen phosphorylase (encoded by the PYGM gene), and myozenin 2 (encoded by the MYOZ2 gene). The validation results were consistent with the TMT results. Conclusion: This is the first proteomics study that has investigated the pathogenesis of chronic myofascial pain and the mechanisms underlying the treatment effects of dry needling in an in vivo rat model of MTrPs, which might promote our understanding of the molecular mechanisms underlying chronic myofascial pain. Keywords: musculoskeletal pain, acupuncture, bioinformatics, mass spectrometry, tandem mass tag, parallel reaction monitoring  Link bài viết: Tham khảo thêm tại đây Structural and functional abnormalities of motor endplates in rat skeletal model of myofascial trigger spots (2019). Authors: Qing-GuangLiu a , Qiang-MinHuang b , LinLiu c , Thi-ThamNguyen d  ABSTRACT  Myofascial trigger points (MTrPs) are defined as hyperirritable spots in a palpable taut band (TB) of skeletal muscle fibers. Knowing the formation and location of MTrPs is a great help to prevent their development and inactivate existing MTrPs. This study aimed to obtain new evidence that myofascial trigger spots (MTrSs), which are similar to human MTrPs, are found in dysfunctional motor endplates by observing the morphological characteristics of muscles and changes in biochemical substances. A total of 32 male Sprague Dawley rats were randomly divided into four groups: two control groups (i.e., C1 and C2) and two model groups (i.e., M1 and M2). C1 and M1 were used for acetylcholine (ACh) content measurement, while C2 and M2 were utilized for acetylcholinesterase (AChE) staining. In the model groups, blunt striking injury was induced and eccentric exercise was applied to the left gastrocnemius for 8 weeks. After 1 month, spontaneous electrical activity(SEA), AChE optical density (OD), muscle fiber diameter, and ACh content were measured. The results showed that extensive abnormal endplate noise (aEPN), including positive neurons, fibrillation potentials, fasciculation potential, and high amplitude (endplate spikes [EPS]), is present at MTrSs in M1. Quantitative electromyography results showed that the amplitudes of aEPN and frequency of EPS in M1 were significantly higher than those of C1. The ACh content of MTrSs in M1 was significantly higher than that in C1. The AChE OD value of M2 was significantly lower than that of C2. In addition, the diameter of the muscle fibers in the AChE-stained area was longer in M2 than in C2. In conclusion, MTrSs formed at the motor endplate with a larger diameter of muscle fibers. Excessive ACh release and decreased AChE activity at MTrSs stimulated muscle action potential and muscle contraction.  Link bài viết: Tham khảo thêm tại đây Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study (2019). Authors: Lihui Li 1,2 , Qiangmin Huang 1 , Marco Barbero 2 , Lin Liu 3 , Thitham Nguyen 4 , Anle Xu 1, and Lijuan Ji 1  ABSTRACT  A quantitative proteomic analysis of the response to dry needling combined with static stretching treatment was performed in a rat model of active myofascial trigger points (MTrPs). 36 rats were divided into a model group (MG), a stretching group (SG) and a dry needling combined with stretching group (SDG). We performed three biological replicates to compare large-scale differential protein expression between groups by tandem mass tag (TMT) labeling technology based on nanoscale liquid chromatography mass spectrometry analysis (LC–MS/MS). Hierarchical clustering, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and protein-protein interaction network analyses were performed for the general characterization of overall enriched proteins. For validation of the results of TMT, the candidate proteins were verified by parallel reaction monitoring (PRM) analysis. 285 differentially expressed proteins between groups were identified and quantified. Tight junction pathway played a dominant role in dry needling combined with static stretching treatment for the rat model of active MTrPs. Three candidate proteins, namely actinin alpha 3, calsequestrin-1 and parvalbumin alpha, were further validated, consistent with the results of LC–MS/MS. This is the first proteomics-based study to report the therapeutic mechanism underlying dry needling and static stretching treatment for MTrPs. Further functional verification of the potential signaling pathways and the enriched proteins is warranted. View Full-Text Keywords: myofascial trigger points; proteomic; chronic myofascial pain; dry needling; stretching; mass spectrometry; tandem mass tag; actinin alpha 3; calsequestrin-1; parvalbumin alpha  Link bài viết: Tham khảo thêm tại đây Relationship between muscle spindles and myofascial trigger spots according to Hoffmann reflex pathway and tissue morphology characteristics in a rat model (2020). Authors: Lin Liu 1 ,  Qiang-Min Huang 2,3 , Qing-Guang Liu 4 , Thi-Tham Nguyen 5 , Jian-Qin Yan 6 , Cheng-Zhi Bo 1 .  ABSTRACT  Objectives: To determine how muscle spindles are involved in the pathophysiology of chronic myofascial trigger spots (MTrSs, similar to myofascial trigger points) in a rat injury model according to the characteristics of the Hoffmann reflex (H-reflex) and the anatomical relationship between muscle spindles and MTrSs. Methods: 16 male Sprague-Dawley rats (7 weeks old) were randomly divided into experimental and control groups. A blunt strike injury and eccentric exercise were applied to the gastrocnemius muscle of rats in the experimental group once a week for 8 weeks as a MTrS modelling intervention. Subsequently, the rats were reared normally and rested for 4 weeks. At the end of the 12th week, the rats were examined for the presence of MTrSs defined by the detection of a palpable taut band exhibiting both a local twitch response and spontaneous electrical activity. After modelling, evocation of the H-reflex and morphological examination of muscle spindles and MTrSs were conducted. Results: The threshold (0.35±0.04 mA) of the H-reflex and latency (1.24±0.18 ms) of the M wave recorded at MTrSs were not significantly different to those at non-MTrSs (P>0.05). Compared with non-MTrSs, a lower Mmax (4.28±1.27 mV), higher Hmax (median (IQR) 0.95 (0.80–1.08) mV) and Hmax/Mmax (median (IQR) 0.21 (0.16–0.40)), and shorter H wave latency (4.60±0.89 ms) were recorded at MTrSs (P<0.05). Morphologically, there was a close anatomical relationship between the MTrS cells and the muscle spindles. Discussion: Compared with normal muscles, the H-reflex myoelectrical activity was enhanced and some muscle spindles might have been influenced by active MTrSs. Thus, muscle spindles may play an important role in the pathological mechanism underlying myofascial trigger points.  Link bài viết: Tham khảo thêm tại đây Nghiên cứu Hội nghị - hội thảo Công trình công bố ngoài nước Công trình công bố trong nước Nghiên cứu khoa học sinh viên Bài viết mới nhất Cuộc thi Sáng tạo Sự kiện mùa 3 -Dream On trở lại đầy mới lạ Sự kiện  / 15 Th4 24 Golfzon Việt Nam đồng hành cùng Khoa KHTT tại TDTU Open Day 2024 Sự kiện nổi bật  / 08 Th4 24 Chương trình gặp gỡ kết nối Doanh nghiệp 2024 Sự kiện  / 01 Th4 24 Xem thêm Thông tin liên hệ Khoa khoa học thể thao - Đại học Tôn Đức Thắng 19 Nguyễn Hữu Thọ, P. Tân Phong, Quận 7, TP. HCM 0283 7755 026  fss@tdtu.edu.vn Liên kết Giới thiệu  Đào tạo  Nghiên cứu  CLB & Đội nhóm  Tuyển sinh Tin tức  Sự kiện  Liên hệ  Facebook  Youtube Liên kết ngoài TDTU Website  INSPiRE Library  Quốc tế hóa  Sau đại học Sinh viên  Đại học Copyright ©  Đại học Tôn Đức Thắng