title: Jennifer's Body

text: Mary Pols of "Time" magazine called the film entertaining and reasoned "[t]here is a lot of intelligent camp here, and some sharply observed characterizations" and Cody and Kusama's "depiction of the ways in which women like Needy are willing to compromise themselves to indulge an ultimately less secure friend is spot-on". Dana Stevens of "Slate" praised the film for being "luscious and powerful, sexy and scary, maddening at times, but impossible to stop watching" and a "wicked black comedy with unexpected emotional resonance, one of the most purely pleasurable movies of the year so far". "Elle"s Karen Durbin said the film not only puts "a fresh spin on female-centric pop genres but also own[s] them outright" and is "rich with first-rate performances".


title: Jennifer's Body

text: "The Miami Herald" Rene Rodriguez likened the film's "[effective exploitation] of the genre as a metaphor for adolescent angst, female sexuality and the strange, sometimes corrosive bonds between girls who claim to be best friends" to Brian De Palma's 1976 film "Carrie". She applauded the film for being fearless when delving into the subject of teen sex and for reversing the notion that only "bad girls have sex when they're 16 [and the] good ones—those who, like Needy, do their homework and are responsible—never slide past first base". Nick Pinkerton of "Sci Fi Weekly" called Fox and Seyfried's lesbian kissing scene "the best close-up girl-girl liplock" since "Cruel Intentions" (1999), and A. O. Scott of "The New York Times" concluded "the movie deserves—and is likely to win—a devoted cult following, despite its flaws" and that "[these flaws] are mitigated by a sensibility that mixes playful pop-culture ingenuity with a healthy shot of feminist anger".


title: Jennifer's Body

text: Giving a partially negative review of the film was Joshua Rothkopf of "Time Out New York", who said the "movie has a centerfold sheen to it—and some lesbianic soft-core flirtation to match—as its plot dives deeply into "Twilight"-esque heavy-melo meltdown in the last act" and that "Cody throws one too many losses at Needy; the screenwriter loses her satiric way about halfway through. But for a while, this has real fangs". Ann Hornaday of "The Washington Post" said, "There's a certain kooky, kinky fun to be had with "Jennifer's Body"" but that "[a]dmittedly, this is the stuff of lurid adolescent distraction, not great cinema" and "is strictly a niche item but provides a goofy, campy bookend to "Drag Me to Hell" (2009) on the B-movie shelf. Watch it, forget it, move on". "San Francisco Chronicle" Peter Hartlaub stated, "Enjoy the film for its witty dialogue and fun performances, but know that there isn't a single good scare. An episode of "Murder, She Wrote" has more thrills." Hartlaub felt the film is not bad, is "almost always pleasing" and that Fox "proves that she has some [acting] range" but "the chances that it will be somebody else's pop culture reference 27 years from now are slim to none". Joe Neumaier of "New York Daily News" said, "Fox merely needs to look either vacant or evil, which the "Transformers" boy-toy does spookily well" but "[w]ords and story are still the lifeblood of a movie, and "Jennifer's Body" is filled like a Twinkie with half-fleshed-out ideas". Disagreeing with Fox's performance, "Chicago Tribune's" Michael Phillips called Fox "a pretty bad actress" who "doesn't seem to get Cody's sense of humor. At all". He reasoned the "movie's partially redeemed by Seyfried, who makes her character more than a repository for audience sympathy" and "her make-out scene with Fox is handled with more suspense and care than anything else in the movie".


title: Jennifer's Body

text: Michael Sragow of "Baltimore Sun" described the only "perfect aspect" of "Jennifer's Body" as being its title. "No one is going to like this movie for its brain", he said. Claudia Puig of "USA Today" stated of the film, ""Jennifer's Body" is not as hot as you hope it would be". Where others praised the film's dialogue, MSNBC's Alonso Duralde called the writing lazy and "[w]orse still, all of Cody's trademark pop-culture–infused dialogue stands out as artificial and precious". "Jennifer's Body", he said, wants "so badly" to be a "Heathers"-esque dark comedy, "but its shortcomings makes you appreciate why that earlier film was so great". Ty Burr of "The Boston Globe" also said the film wants to be like "Heathers", and reminded him "a lot" of "Heathers" but the only scene in the film that "actually feels dangerous" is when the possessed Jennifer initiates a long and passionate kiss with Needy, which the film "very, very nervously backs away from" and that ""Jennifer's Body" falls into the dispiriting category of dumb movies made by smart people, in this case a glibly clever writer and a talented director who think a few wisecracks are enough to subvert the teen horror genre".


title: Jennifer's Body

text: In 2018, Constance Grady reported in "Vox" that a new critical consensus was forming that appreciated the film as a "forgotten feminist classic". She stated that after the Me Too movement highlighted routine sexual harassment and misconduct in the media industry, the film's story of "a group of powerful men sacrificing a girl's body on the altar of their own professional advancement" became "uncomfortably familiar." This, according to Grady, allowed viewers to see the film, rather than as a sex fantasy, as a revenge fantasy as Jennifer uses her abused body against her attackers.


title: Jennifer's Body

text: According to the screenwriter, Diablo Cody, the film was marketed "all wrong". She had argued with executives who wanted "to market this to boys who like Megan Fox. That's who's going to see it. And I was like. No! This is a movie for girls too! That audience, they did not attempt to reach."


title: Jennifer's Body

text: In a 2022 essay on its status as a classic queer and bisexual film, Carmen Maria Machado wrote that "Jennifer's Body" speaks to "what it means to experience parallel sexualities with your best friend as you punch through the last vestiges of childhood." Machado rejects accusations that the film is guilty of queerbaiting, considering it instead an effective depiction of the "central body of water that is bisexuality" which many queer people spend at least some of their life in.


title: Jennifer's Body

text: Though the film was expected to pull in a significant number of the late teenage/young adult audience, particularly males aged 17 and older, and though Cody hoped for a large female turnout, it earned a "disappointing" $2.8 million on its opening Friday and $6.8 million its opening weekend at the North American box office; the film placed #5, while 3D animated film "Cloudy with a Chance of Meatballs "placed #1 with $30.1 million. Produced for $16 million, "Jennifer's Body" did manage to attract the sizable female audience Cody wanted; 51% were female, with 70% of patrons under age 25. The film had been expected to benefit somewhat from its heavily marketed lesbian kissing scene between Fox and Seyfried, which, in addition to Fox being in the film, was thought to entice and successfully attract male viewers. Critic Jim Vejvoda at IGN stated that such a scene is not as shocking as it was in past decades and cannot be expected to significantly pull in an audience. The film grossed $16,204,793 domestically and $15,351,268 in international sales, for a worldwide total of $31,556,061.


title: Jennifer's Body

text: Box-office analysts and critics debated the film's underperformance. Analyst Jeff Bock, of Exhibitor Relations, reasoned the film underperformed at the box office due to two reasons; the first, he said, is the genre. Bock stated that Americans get horror and comedy, but with the idea "of those two things together in one place, people suddenly get very dumb". "The horror-comedy genre is the toughest sell in Hollywood", he said. He noted films "Tremors", "Slither", "Shaun of the Dead", "Eight Legged Freaks" and "The Evil Dead" series, and said that while many of those are considered critical and business successes, "none of them have brought in the megabucks that a simple horror or comedy can." In addition, he labeled the "Scream" franchise as more "straight-up horror" than comedy and stated "Zombieland"s box office performance would determine the horror-comedy genre's current viability.


title: Jennifer's Body

text: Despite other R-rated horror films having centered around teenagers, some such as "Scream" having been successful, Bock said the second reason "Jennifer's Body" under-performed at the box office is the R-rating, which he described as a "killer" for the film. He said the film is set in high school and "sounds like the perfect package for teens" but that "the R rating banned many teens from the theaters" and the studio was left with "an R-rated film marketed to whom, exactly?" Nicole Sperling of "Entertainment Weekly" felt that it was a slow and disappointing weekend for the box office in general; 3D animated film "Cloudy with a Chance of Meatballs" was steep competition, and with low box office performances by the Matt Damon film "The Informant!" and Jennifer Aniston film "Love Happens", she concluded that this may support "the current hypothesis floating around Hollywood, that movie stars no longer matter" and that it takes more than a name to open a film. S.T. VanAirsdale of "Movieline" echoed Sperling's sentiment about the weekend, as "some of the stinkiest high-profile openings in recent memory". He concluded five reasons for "Jennifer's Body"s underperformance at the box office. The first, he said, is the distributor. "20th Century Fox's genre wing, Fox Atomic, had "Jennifer's Body" in the can by the time the mother ship shut it down last spring", he said. "Instead of offloading the film to Fox Searchlight, which nimbly maneuvered Diablo Cody's previous brainchild "Juno" to awards-season lucre in 2007, a decision was somehow reached to fill a gap in Big Fox's early fall slate with a gory" and "post-feminist horror romp starring Megan Fox and Amanda Seyfried". VanAirsdale classified this as a "[b]ig mistake" and that "[y]ou'd have to go back to "The Devil Wears Prada" to find an example of a Fox release that worked without a genuine male lead; you'd probably have to go back to "Aliens" to find a genre example of such that they pulled off successfully." He named the second reason as the marketing, stating that the film was not well-marketed (whether by billboards, transit posters, lobby standees, or other promotional venues), even in New York.


title: Jennifer's Body

text: VanAirsdale cited the release date and screening as the third and fourth reasons; he said there was confusion about what day the film was going to debut in theaters, and that Toronto is "a nation removed from the audience where the film's actual momentum had been accruing for at least a month" and that "this rarely works for early fall releases; not because news doesn't travel, obviously, but because it peels away a layer of accessibility that accompanies New York and L.A. bows." The final reason, he attributed to the critics, believing that the fusion of horror and teen comedy confused some of them. He mentioned Ty Burr's review in particular, and stated that the film could perhaps have used more horror and been funnier, but that the film is "ultimately a movie about two teenage girls' misadventures in victimization" and that "[t]he jokes are virtually incidental to the friction imposed on women who happen to be two sides of the same coin. Who's the monster, and who made the monster? Sorry if you wanted "Heathers" with demons, fellas. Equipment's cheap these days; perhaps make your own?"


title: Jennifer's Body

text: Hollywood.com box-office analyst and President Paul Dergarabedian said "the poor numbers don't mean Fox can't open a movie." "It may be a matter of just choosing the right projects for her", he told Associated Press"." "She's trying to find a world beyond "Transformers", and she will. She's young and has a lot of promise."


title: Jennifer's Body

text: The film was released on DVD and Blu-ray on December 29, 2009, in the United States and Canada. In Australia, the DVD and Blu-ray was released on May 18, 2010. The film opened at #11 at the DVD sales chart, making $1.6 million in the first week off 104,000 DVD units. There is a rated and an unrated version of the film, with the unrated version running about five minutes longer than the theatrical version. The UK Blu-ray lacks most of the extras found on the locked US release.


title: Jennifer's Body

text: The film's soundtrack was released by Fueled by Ramen on August 25, 2009, and featured previously released music by various indie rock and alternative rock bands such as White Lies, Florence + The Machine, Silversun Pickups and Black Kids. It also features pop punk band All Time Low and electropop singer Little Boots. In addition, the album features new songs from pop rock artists such as Cobra Starship and Panic! at the Disco and Paramore's lead singer Hayley Williams. The first single from the soundtrack is "New Perspective" by Panic! at the Disco.


title: Jennifer's Body

text: The album received a 3 out of 5 review from Allmusic, who described the album as having "a slightly different spin, mixing indie with the more expected punk, emo, and metal". Mike Diver at the BBC wrote an unfavorable review of the album, stating that "This assortment of acts says nothing of its parent film, beyond the occasional reference to school days and nods to something nasty coming this way".


title: Jennifer's Body

text: The ending sequence of the film itself features a song, "Violet", from the album "Live Through This" by Hole. This same album also features a song entitled "Jennifer's Body." In total, the film features 22 songs, most of which are included on the soundtrack.


title: Jennifer's Body

text: As a tie-in to the film, Boom! Studios produced a "Jennifer's Body" graphic novel. The graphic novel expands on the film's universe and Jennifer's murders of the boys. It was written by Black Metal's Rick Spears, with the first nine pages illustrated by Jim Mahfood ("Clerks"). Two covers, in Fox's likeness, were designed; one for the direct market by Eric Jones (available only in comic specialty stores), and the other by Frank Cho for the mass market focusing more on "hellish Jennifer stories" with art by Mahfood, Hack/Slash's Tim Seely, DMZ's Nikki Cook, and Popgun's Ming Doyle. The novel was released in August 2009.


title: Jennifer's Body

text: The novel features less of Jennifer than the film, but does capture her "going in for the kill" several times. It focuses heavily on following her soon-to-be victims and provides information on their personalities not elaborated on in the film so that readers can better conclude whether the boys deserved to be murdered. The novel consists of four chapters, with a prologue and an epilogue, with art provided for each by different artists. Each one follows a different boy and what is happening in his life just before Jennifer kills him.


title: Jennifer's Body

text: On creating the story, Spears stated, "The best part for me as a writer was to show some events from the movie from a different point of view, sort of like "Rashomon" for you Kurosawa fans. And with comics we can get into the character's ["sic"] heads in a way that works well in comics and novels more so than in film." He stated, "... I was using the medium to change what we really know about these characters and twist around what we see in the movies. All the academics aside, it's also very funny and gore splattered."


title: Jennifer's Body

text: Spears stated that while writing the stories, the film was still being made and he had not seen any of it at the time. He mainly learned about the characters through the script. "I got to read the screenplay. It was kinda crazy writing characters that were being changed on set and in the editing process. I had to bob and weave to keep up but that was all part of the fun", he said.


title: Protein

text: Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, responding to stimuli, providing structure to cells and organisms, and transporting molecules from one location to another. Proteins differ from one another primarily in their sequence of amino acids, which is dictated by the nucleotide sequence of their genes, and which usually results in protein folding into a specific 3D structure that determines its activity.


title: Protein

text: A linear chain of amino acid residues is called a polypeptide. A protein contains at least one long polypeptide. Short polypeptides, containing less than 20–30 residues, are rarely considered to be proteins and are commonly called peptides. The individual amino acid residues are bonded together by peptide bonds and adjacent amino acid residues. The sequence of amino acid residues in a protein is defined by the sequence of a gene, which is encoded in the genetic code. In general, the genetic code specifies 20 standard amino acids; but in certain organisms the genetic code can include selenocysteine and—in certain archaea—pyrrolysine. Shortly after or even during synthesis, the residues in a protein are often chemically modified by post-translational modification, which alters the physical and chemical properties, folding, stability, activity, and ultimately, the function of the proteins. Some proteins have non-peptide groups attached, which can be called prosthetic groups or cofactors. Proteins can also work together to achieve a particular function, and they often associate to form stable protein complexes.


title: Protein

text: Once formed, proteins only exist for a certain period and are then degraded and recycled by the cell's machinery through the process of protein turnover. A protein's lifespan is measured in terms of its half-life and covers a wide range. They can exist for minutes or years with an average lifespan of 1–2 days in mammalian cells. Abnormal or misfolded proteins are degraded more rapidly either due to being targeted for destruction or due to being unstable.


title: Protein

text: Like other biological macromolecules such as polysaccharides and nucleic acids, proteins are essential parts of organisms and participate in virtually every process within cells. Many proteins are enzymes that catalyse biochemical reactions and are vital to metabolism. Proteins also have structural or mechanical functions, such as actin and myosin in muscle and the proteins in the cytoskeleton, which form a system of scaffolding that maintains cell shape. Other proteins are important in cell signaling, immune responses, cell adhesion, and the cell cycle. In animals, proteins are needed in the diet to provide the essential amino acids that cannot be synthesized. Digestion breaks the proteins down for metabolic use.


title: Protein

text: Proteins may be purified from other cellular components using a variety of techniques such as ultracentrifugation, precipitation, electrophoresis, and chromatography; the advent of genetic engineering has made possible a number of methods to facilitate purification. Methods commonly used to study protein structure and function include immunohistochemistry, site-directed mutagenesis, X-ray crystallography, nuclear magnetic resonance and mass spectrometry.


title: Protein

text: Proteins were recognized as a distinct class of biological molecules in the eighteenth century by Antoine Fourcroy and others, distinguished by the molecules' ability to coagulate or flocculate under treatments with heat or acid. Noted examples at the time included albumin from egg whites, blood serum albumin, fibrin, and wheat gluten.


title: Protein

text: Proteins were first described by the Dutch chemist Gerardus Johannes Mulder and named by the Swedish chemist Jöns Jacob Berzelius in 1838. Mulder carried out elemental analysis of common proteins and found that nearly all proteins had the same empirical formula, CHNOPS. He came to the erroneous conclusion that they might be composed of a single type of (very large) molecule. The term "protein" to describe these molecules was proposed by Mulder's associate Berzelius; protein is derived from the Greek word (), meaning "primary", "in the lead", or "standing in front", + "-in". Mulder went on to identify the products of protein degradation such as the amino acid leucine for which he found a (nearly correct) molecular weight of 131 Da. Prior to "protein", other names were used, like "albumins" or "albuminous materials" ("Eiweisskörper", in German).


title: Protein

text: Early nutritional scientists such as the German Carl von Voit believed that protein was the most important nutrient for maintaining the structure of the body, because it was generally believed that "flesh makes flesh." Karl Heinrich Ritthausen extended known protein forms with the identification of glutamic acid. At the Connecticut Agricultural Experiment Station a detailed review of the vegetable proteins was compiled by Thomas Burr Osborne. Working with Lafayette Mendel and applying Liebig's law of the minimum in feeding laboratory rats, the nutritionally essential amino acids were established. The work was continued and communicated by William Cumming Rose. The understanding of proteins as polypeptides came through the work of Franz Hofmeister and Hermann Emil Fischer in 1902. The central role of proteins as enzymes in living organisms was not fully appreciated until 1926, when James B. Sumner showed that the enzyme urease was in fact a protein.


title: Protein

text: The difficulty in purifying proteins in large quantities made them very difficult for early protein biochemists to study. Hence, early studies focused on proteins that could be purified in large quantities, e.g., those of blood, egg white, various toxins, and digestive/metabolic enzymes obtained from slaughterhouses. In the 1950s, the Armour Hot Dog Co. purified 1 kg of pure bovine pancreatic ribonuclease A and made it freely available to scientists; this gesture helped ribonuclease A become a major target for biochemical study for the following decades.


title: Protein

text: Linus Pauling is credited with the successful prediction of regular protein secondary structures based on hydrogen bonding, an idea first put forth by William Astbury in 1933. Later work by Walter Kauzmann on denaturation, based partly on previous studies by Kaj Linderstrøm-Lang, contributed an understanding of protein folding and structure mediated by hydrophobic interactions.


title: Protein

text: The first protein to be sequenced was insulin, by Frederick Sanger, in 1949. Sanger correctly determined the amino acid sequence of insulin, thus conclusively demonstrating that proteins consisted of linear polymers of amino acids rather than branched chains, colloids, or cyclols. He won the Nobel Prize for this achievement in 1958.


title: Protein

text: With the development of X-ray crystallography, it became possible to sequence protein structures. The first protein structures to be solved were hemoglobin by Max Perutz and myoglobin by John Kendrew, in 1958. The use of computers and increasing computing power also supported the sequencing of complex proteins. In 1999, Roger Kornberg succeeded in sequencing the highly complex structure of RNA polymerase using high intensity X-rays from synchrotrons.


title: Protein

text: Since then, cryo-electron microscopy (cryo-EM) of large macromolecular assemblies has been developed. Cryo-EM uses protein samples that are frozen rather than crystals, and beams of electrons rather than x-rays. It causes less damage to the sample, allowing scientists to obtain more information and analyze larger structures. Computational protein structure prediction of small protein domains has also helped researchers to approach atomic-level resolution of protein structures.


title: Protein

text: The number of proteins encoded in a genome roughly corresponds to the number of genes (although there may be a significant number of genes that encode RNA of protein, e.g. ribosomal RNAs). Viruses typically encode a few to a few hundred proteins, archaea and bacteria a few hundred to a few thousand, while eukaryotes typically encode a few thousand up to tens of thousands of proteins (see genome size for a list of examples).


title: Protein

text: Most proteins consist of linear polymers built from series of up to 20 different -α- amino acids. All proteinogenic amino acids possess common structural features, including an α-carbon to which an amino group, a carboxyl group, and a variable side chain are bonded. Only proline differs from this basic structure as it contains an unusual ring to the N-end amine group, which forces the CO–NH amide moiety into a fixed conformation. The side chains of the standard amino acids, detailed in the list of standard amino acids, have a great variety of chemical structures and properties; it is the combined effect of all of the amino acid side chains in a protein that ultimately determines its three-dimensional structure and its chemical reactivity.


title: Protein

text: The amino acids in a polypeptide chain are linked by peptide bonds. Once linked in the protein chain, an individual amino acid is called a "residue," and the linked series of carbon, nitrogen, and oxygen atoms are known as the "main chain" or "protein backbone."


title: Protein

text: The peptide bond has two resonance forms that contribute some double-bond character and inhibit rotation around its axis, so that the alpha carbons are roughly coplanar. The other two dihedral angles in the peptide bond determine the local shape assumed by the protein backbone. The end with a free amino group is known as the N-terminus or amino terminus, whereas the end of the protein with a free carboxyl group is known as the C-terminus or carboxy terminus (the sequence of the protein is written from N-terminus to C-terminus, from left to right).


title: Protein

text: The words "protein", "polypeptide," and "peptide" are a little ambiguous and can overlap in meaning. "Protein" is generally used to refer to the complete biological molecule in a stable conformation, whereas "peptide" is generally reserved for a short amino acid oligomers often lacking a stable 3D structure. But the boundary between the two is not well defined and usually lies near 20–30 residues. "Polypeptide" can refer to any single linear chain of amino acids, usually regardless of length, but often implies an absence of a defined conformation.


title: Protein

text: Proteins can interact with many types of molecules, including with other proteins, with lipids, with carbohydrates, and with DNA.


title: Protein

text: It has been estimated that average-sized bacteria contain about 2 million proteins per cell (e.g. "E. coli" and "Staphylococcus aureus"). Smaller bacteria, such as "Mycoplasma" or "spirochetes" contain fewer molecules, on the order of 50,000 to 1 million. By contrast, eukaryotic cells are larger and thus contain much more protein. For instance, yeast cells have been estimated to contain about 50 million proteins and human cells on the order of 1 to 3 billion. The concentration of individual protein copies ranges from a few molecules per cell up to 20 million. Not all genes coding proteins are expressed in most cells and their number depends on, for example, cell type and external stimuli. For instance, of the 20,000 or so proteins encoded by the human genome, only 6,000 are detected in lymphoblastoid cells.


title: Protein

text: Proteins are assembled from amino acids using information encoded in genes. Each protein has its own unique amino acid sequence that is specified by the nucleotide sequence of the gene encoding this protein. The genetic code is a set of three-nucleotide sets called codons and each three-nucleotide combination designates an amino acid, for example AUG (adenine–uracil–guanine) is the code for methionine. Because DNA contains four nucleotides, the total number of possible codons is 64; hence, there is some redundancy in the genetic code, with some amino acids specified by more than one codon. Genes encoded in DNA are first transcribed into pre-messenger RNA (mRNA) by proteins such as RNA polymerase. Most organisms then process the pre-mRNA (also known as a "primary transcript") using various forms of Post-transcriptional modification to form the mature mRNA, which is then used as a template for protein synthesis by the ribosome. In prokaryotes the mRNA may either be used as soon as it is produced, or be bound by a ribosome after having moved away from the nucleoid. In contrast, eukaryotes make mRNA in the cell nucleus and then translocate it across the nuclear membrane into the cytoplasm, where protein synthesis then takes place. The rate of protein synthesis is higher in prokaryotes than eukaryotes and can reach up to 20 amino acids per second.


title: Protein

text: The process of synthesizing a protein from an mRNA template is known as translation. The mRNA is loaded onto the ribosome and is read three nucleotides at a time by matching each codon to its base pairing anticodon located on a transfer RNA molecule, which carries the amino acid corresponding to the codon it recognizes. The enzyme aminoacyl tRNA synthetase "charges" the tRNA molecules with the correct amino acids. The growing polypeptide is often termed the "nascent chain". Proteins are always biosynthesized from N-terminus to C-terminus.


title: Protein

text: The size of a synthesized protein can be measured by the number of amino acids it contains and by its total molecular mass, which is normally reported in units of "daltons" (synonymous with atomic mass units), or the derivative unit kilodalton (kDa). The average size of a protein increases from Archaea to Bacteria to Eukaryote (283, 311, 438 residues and 31, 34, 49 kDa respectively) due to a bigger number of protein domains constituting proteins in higher organisms. For instance, yeast proteins are on average 466 amino acids long and 53 kDa in mass. The largest known proteins are the titins, a component of the muscle sarcomere, with a molecular mass of almost 3,000 kDa and a total length of almost 27,000 amino acids.


title: Protein

text: Short proteins can also be synthesized chemically by a family of methods known as peptide synthesis, which rely on organic synthesis techniques such as chemical ligation to produce peptides in high yield. Chemical synthesis allows for the introduction of non-natural amino acids into polypeptide chains, such as attachment of fluorescent probes to amino acid side chains. These methods are useful in laboratory biochemistry and cell biology, though generally not for commercial applications. Chemical synthesis is inefficient for polypeptides longer than about 300 amino acids, and the synthesized proteins may not readily assume their native tertiary structure. Most chemical synthesis methods proceed from C-terminus to N-terminus, opposite the biological reaction.


title: Protein

text: Most proteins fold into unique 3D structures. The shape into which a protein naturally folds is known as its native conformation. Although many proteins can fold unassisted, simply through the chemical properties of their amino acids, others require the aid of molecular chaperones to fold into their native states. Biochemists often refer to four distinct aspects of a protein's structure:


title: Protein

text: Proteins are not entirely rigid molecules. In addition to these levels of structure, proteins may shift between several related structures while they perform their functions. In the context of these functional rearrangements, these tertiary or quaternary structures are usually referred to as "conformations", and transitions between them are called "conformational changes." Such changes are often induced by the binding of a substrate molecule to an enzyme's active site, or the physical region of the protein that participates in chemical catalysis. In solution proteins also undergo variation in structure through thermal vibration and the collision with other molecules.


title: Protein

text: Proteins can be informally divided into three main classes, which correlate with typical tertiary structures: globular proteins, fibrous proteins, and membrane proteins. Almost all globular proteins are soluble and many are enzymes. Fibrous proteins are often structural, such as collagen, the major component of connective tissue, or keratin, the protein component of hair and nails. Membrane proteins often serve as receptors or provide channels for polar or charged molecules to pass through the cell membrane.


title: Protein

text: A special case of intramolecular hydrogen bonds within proteins, poorly shielded from water attack and hence promoting their own dehydration, are called dehydrons.


title: Protein

text: Many proteins are composed of several protein domains, i.e. segments of a protein that fold into distinct structural units. Domains usually also have specific functions, such as enzymatic activities (e.g. kinase) or they serve as binding modules (e.g. the SH3 domain binds to proline-rich sequences in other proteins).


title: Protein

text: Short amino acid sequences within proteins often act as recognition sites for other proteins. For instance, SH3 domains typically bind to short PxxP motifs (i.e. 2 prolines [P], separated by two unspecified amino acids [x], although the surrounding amino acids may determine the exact binding specificity). Many such motifs has been collected in the Eukaryotic Linear Motif (ELM) database.


title: Protein

text: Proteins are the chief actors within the cell, said to be carrying out the duties specified by the information encoded in genes. With the exception of certain types of RNA, most other biological molecules are relatively inert elements upon which proteins act. Proteins make up half the dry weight of an "Escherichia coli" cell, whereas other macromolecules such as DNA and RNA make up only 3% and 20%, respectively. The set of proteins expressed in a particular cell or cell type is known as its proteome.


title: Protein

text: The chief characteristic of proteins that also allows their diverse set of functions is their ability to bind other molecules specifically and tightly. The region of the protein responsible for binding another molecule is known as the binding site and is often a depression or "pocket" on the molecular surface. This binding ability is mediated by the tertiary structure of the protein, which defines the binding site pocket, and by the chemical properties of the surrounding amino acids' side chains. Protein binding can be extraordinarily tight and specific; for example, the ribonuclease inhibitor protein binds to human angiogenin with a sub-femtomolar dissociation constant (<10 M) but does not bind at all to its amphibian homolog onconase (>1 M). Extremely minor chemical changes such as the addition of a single methyl group to a binding partner can sometimes suffice to nearly eliminate binding; for example, the aminoacyl tRNA synthetase specific to the amino acid valine discriminates against the very similar side chain of the amino acid isoleucine.


title: Protein

text: Proteins can bind to other proteins as well as to small-molecule substrates. When proteins bind specifically to other copies of the same molecule, they can oligomerize to form fibrils; this process occurs often in structural proteins that consist of globular monomers that self-associate to form rigid fibers. Protein–protein interactions also regulate enzymatic activity, control progression through the cell cycle, and allow the assembly of large protein complexes that carry out many closely related reactions with a common biological function. Proteins can also bind to, or even be integrated into, cell membranes. The ability of binding partners to induce conformational changes in proteins allows the construction of enormously complex signaling networks.


title: Protein

text: As interactions between proteins are reversible, and depend heavily on the availability of different groups of partner proteins to form aggregates that are capable to carry out discrete sets of function, study of the interactions between specific proteins is a key to understand important aspects of cellular function, and ultimately the properties that distinguish particular cell types.


title: Protein

text: The best-known role of proteins in the cell is as enzymes, which catalyse chemical reactions. Enzymes are usually highly specific and accelerate only one or a few chemical reactions. Enzymes carry out most of the reactions involved in metabolism, as well as manipulating DNA in processes such as DNA replication, DNA repair, and transcription. Some enzymes act on other proteins to add or remove chemical groups in a process known as posttranslational modification. About 4,000 reactions are known to be catalysed by enzymes. The rate acceleration conferred by enzymatic catalysis is often enormous—as much as 10-fold increase in rate over the uncatalysed reaction in the case of orotate decarboxylase (78 million years without the enzyme, 18 milliseconds with the enzyme).


title: Protein

text: The molecules bound and acted upon by enzymes are called substrates. Although enzymes can consist of hundreds of amino acids, it is usually only a small fraction of the residues that come in contact with the substrate, and an even smaller fraction—three to four residues on average—that are directly involved in catalysis. The region of the enzyme that binds the substrate and contains the catalytic residues is known as the active site.


title: Protein

text: Dirigent proteins are members of a class of proteins that dictate the stereochemistry of a compound synthesized by other enzymes.


title: Protein

text: Many proteins are involved in the process of cell signaling and signal transduction. Some proteins, such as insulin, are extracellular proteins that transmit a signal from the cell in which they were synthesized to other cells in distant tissues. Others are membrane proteins that act as receptors whose main function is to bind a signaling molecule and induce a biochemical response in the cell. Many receptors have a binding site exposed on the cell surface and an effector domain within the cell, which may have enzymatic activity or may undergo a conformational change detected by other proteins within the cell.


title: Protein

text: Antibodies are protein components of an adaptive immune system whose main function is to bind antigens, or foreign substances in the body, and target them for destruction. Antibodies can be secreted into the extracellular environment or anchored in the membranes of specialized B cells known as plasma cells. Whereas enzymes are limited in their binding affinity for their substrates by the necessity of conducting their reaction, antibodies have no such constraints. An antibody's binding affinity to its target is extraordinarily high.


title: Protein

text: Many ligand transport proteins bind particular small biomolecules and transport them to other locations in the body of a multicellular organism. These proteins must have a high binding affinity when their ligand is present in high concentrations, but must also release the ligand when it is present at low concentrations in the target tissues. The canonical example of a ligand-binding protein is haemoglobin, which transports oxygen from the lungs to other organs and tissues in all vertebrates and has close homologs in every biological kingdom. Lectins are sugar-binding proteins which are highly specific for their sugar moieties. Lectins typically play a role in biological recognition phenomena involving cells and proteins. Receptors and hormones are highly specific binding proteins.


title: Protein

text: Transmembrane proteins can also serve as ligand transport proteins that alter the permeability of the cell membrane to small molecules and ions. The membrane alone has a hydrophobic core through which polar or charged molecules cannot diffuse. Membrane proteins contain internal channels that allow such molecules to enter and exit the cell. Many ion channel proteins are specialized to select for only a particular ion; for example, potassium and sodium channels often discriminate for only one of the two ions.


title: Protein

text: Structural proteins confer stiffness and rigidity to otherwise-fluid biological components. Most structural proteins are fibrous proteins; for example, collagen and elastin are critical components of connective tissue such as cartilage, and keratin is found in hard or filamentous structures such as hair, nails, feathers, hooves, and some animal shells. Some globular proteins can also play structural functions, for example, actin and tubulin are globular and soluble as monomers, but polymerize to form long, stiff fibers that make up the cytoskeleton, which allows the cell to maintain its shape and size.


title: Protein

text: Other proteins that serve structural functions are motor proteins such as myosin, kinesin, and dynein, which are capable of generating mechanical forces. These proteins are crucial for cellular motility of single celled organisms and the sperm of many multicellular organisms which reproduce sexually. They also generate the forces exerted by contracting muscles and play essential roles in intracellular transport.


title: Protein

text: A key question in molecular biology is how proteins evolve, i.e. how can mutations (or rather changes in amino acid sequence) lead to new structures and functions? Most amino acids in a protein can be changed without disrupting activity or function, as can be seen from numerous homologous proteins across species (as collected in specialized databases for protein families, e.g. PFAM). In order to prevent dramatic consequences of mutations, a gene may be duplicated before it can mutate freely. However, this can also lead to complete loss of gene function and thus pseudo-genes. More commonly, single amino acid changes have limited consequences although some can change protein function substantially, especially in enzymes. For instance, many enzymes can change their substrate specificity by one or a few mutations. Changes in substrate specificity are facilitated by "substrate promiscuity", i.e. the ability of many enzymes to bind and process multiple substrates. When mutations occur, the specificity of an enzyme can increase (or decrease) and thus its enzymatic activity. Thus, bacteria (or other organisms) can adapt to different food sources, including unnatural substrates such as plastic.


title: Protein

text: The activities and structures of proteins may be examined "in vitro," "in vivo, and in silico". In vitro studies of purified proteins in controlled environments are useful for learning how a protein carries out its function: for example, enzyme kinetics studies explore the chemical mechanism of an enzyme's catalytic activity and its relative affinity for various possible substrate molecules. By contrast, in vivo experiments can provide information about the physiological role of a protein in the context of a cell or even a whole organism. In silico studies use computational methods to study proteins.


title: Protein

text: To perform "in vitro" analysis, a protein must be purified away from other cellular components. This process usually begins with cell lysis, in which a cell's membrane is disrupted and its internal contents released into a solution known as a crude lysate. The resulting mixture can be purified using ultracentrifugation, which fractionates the various cellular components into fractions containing soluble proteins; membrane lipids and proteins; cellular organelles, and nucleic acids. Precipitation by a method known as salting out can concentrate the proteins from this lysate. Various types of chromatography are then used to isolate the protein or proteins of interest based on properties such as molecular weight, net charge and binding affinity. The level of purification can be monitored using various types of gel electrophoresis if the desired protein's molecular weight and isoelectric point are known, by spectroscopy if the protein has distinguishable spectroscopic features, or by enzyme assays if the protein has enzymatic activity. Additionally, proteins can be isolated according to their charge using electrofocusing.


title: Protein

text: For natural proteins, a series of purification steps may be necessary to obtain protein sufficiently pure for laboratory applications. To simplify this process, genetic engineering is often used to add chemical features to proteins that make them easier to purify without affecting their structure or activity. Here, a "tag" consisting of a specific amino acid sequence, often a series of histidine residues (a "His-tag"), is attached to one terminus of the protein. As a result, when the lysate is passed over a chromatography column containing nickel, the histidine residues ligate the nickel and attach to the column while the untagged components of the lysate pass unimpeded. A number of different tags have been developed to help researchers purify specific proteins from complex mixtures.


title: Protein

text: The study of proteins "in vivo" is often concerned with the synthesis and localization of the protein within the cell. Although many intracellular proteins are synthesized in the cytoplasm and membrane-bound or secreted proteins in the endoplasmic reticulum, the specifics of how proteins are targeted to specific organelles or cellular structures is often unclear. A useful technique for assessing cellular localization uses genetic engineering to express in a cell a fusion protein or chimera consisting of the natural protein of interest linked to a "reporter" such as green fluorescent protein (GFP). The fused protein's position within the cell can be cleanly and efficiently visualized using microscopy, as shown in the figure opposite.


title: Protein

text: Other methods for elucidating the cellular location of proteins requires the use of known compartmental markers for regions such as the ER, the Golgi, lysosomes or vacuoles, mitochondria, chloroplasts, plasma membrane, etc. With the use of fluorescently tagged versions of these markers or of antibodies to known markers, it becomes much simpler to identify the localization of a protein of interest. For example, indirect immunofluorescence will allow for fluorescence colocalization and demonstration of location. Fluorescent dyes are used to label cellular compartments for a similar purpose.


title: Protein

text: Other possibilities exist, as well. For example, immunohistochemistry usually uses an antibody to one or more proteins of interest that are conjugated to enzymes yielding either luminescent or chromogenic signals that can be compared between samples, allowing for localization information. Another applicable technique is cofractionation in sucrose (or other material) gradients using isopycnic centrifugation. While this technique does not prove colocalization of a compartment of known density and the protein of interest, it does increase the likelihood, and is more amenable to large-scale studies.


title: Protein

text: Finally, the gold-standard method of cellular localization is immunoelectron microscopy. This technique also uses an antibody to the protein of interest, along with classical electron microscopy techniques. The sample is prepared for normal electron microscopic examination, and then treated with an antibody to the protein of interest that is conjugated to an extremely electro-dense material, usually gold. This allows for the localization of both ultrastructural details as well as the protein of interest.


title: Protein

text: Through another genetic engineering application known as site-directed mutagenesis, researchers can alter the protein sequence and hence its structure, cellular localization, and susceptibility to regulation. This technique even allows the incorporation of unnatural amino acids into proteins, using modified tRNAs, and may allow the rational design of new proteins with novel properties.


title: Protein

text: The total complement of proteins present at a time in a cell or cell type is known as its proteome, and the study of such large-scale data sets defines the field of proteomics, named by analogy to the related field of genomics. Key experimental techniques in proteomics include 2D electrophoresis, which allows the separation of many proteins, mass spectrometry, which allows rapid high-throughput identification of proteins and sequencing of peptides (most often after in-gel digestion), protein microarrays, which allow the detection of the relative levels of the various proteins present in a cell, and two-hybrid screening, which allows the systematic exploration of protein–protein interactions. The total complement of biologically possible such interactions is known as the interactome. A systematic attempt to determine the structures of proteins representing every possible fold is known as structural genomics.


title: Protein

text: Discovering the tertiary structure of a protein, or the quaternary structure of its complexes, can provide important clues about how the protein performs its function and how it can be affected, i.e. in drug design. As proteins are too small to be seen under a light microscope, other methods have to be employed to determine their structure. Common experimental methods include X-ray crystallography and NMR spectroscopy, both of which can produce structural information at atomic resolution. However, NMR experiments are able to provide information from which a subset of distances between pairs of atoms can be estimated, and the final possible conformations for a protein are determined by solving a distance geometry problem. Dual polarisation interferometry is a quantitative analytical method for measuring the overall protein conformation and conformational changes due to interactions or other stimulus. Circular dichroism is another laboratory technique for determining internal β-sheet / α-helical composition of proteins. Cryoelectron microscopy is used to produce lower-resolution structural information about very large protein complexes, including assembled viruses; a variant known as electron crystallography can also produce high-resolution information in some cases, especially for two-dimensional crystals of membrane proteins. Solved structures are usually deposited in the Protein Data Bank (PDB), a freely available resource from which structural data about thousands of proteins can be obtained in the form of Cartesian coordinates for each atom in the protein.


title: Protein

text: Many more gene sequences are known than protein structures. Further, the set of solved structures is biased toward proteins that can be easily subjected to the conditions required in X-ray crystallography, one of the major structure determination methods. In particular, globular proteins are comparatively easy to crystallize in preparation for X-ray crystallography. Membrane proteins and large protein complexes, by contrast, are difficult to crystallize and are underrepresented in the PDB. Structural genomics initiatives have attempted to remedy these deficiencies by systematically solving representative structures of major fold classes. Protein structure prediction methods attempt to provide a means of generating a plausible structure for proteins whose structures have not been experimentally determined.


title: Protein

text: Complementary to the field of structural genomics, "protein structure prediction" develops efficient mathematical models of proteins to computationally predict the molecular formations in theory, instead of detecting structures with laboratory observation. The most successful type of structure prediction, known as homology modeling, relies on the existence of a "template" structure with sequence similarity to the protein being modeled; structural genomics' goal is to provide sufficient representation in solved structures to model most of those that remain. Although producing accurate models remains a challenge when only distantly related template structures are available, it has been suggested that sequence alignment is the bottleneck in this process, as quite accurate models can be produced if a "perfect" sequence alignment is known. Many structure prediction methods have served to inform the emerging field of protein engineering, in which novel protein folds have already been designed. Also proteins (in eukaryotes ~33%) contain large unstructured but biologically functional segments and can be classified as intrinsically disordered proteins. Predicting and analysing protein disorder is, therefore, an important part of protein structure characterisation.


title: Protein

text: A vast array of computational methods have been developed to analyze the structure, function and evolution of proteins. The development of such tools has been driven by the large amount of genomic and proteomic data available for a variety of organisms, including the human genome. It is simply impossible to study all proteins experimentally, hence only a few are subjected to laboratory experiments while computational tools are used to extrapolate to similar proteins. Such homologous proteins can be efficiently identified in distantly related organisms by sequence alignment. Genome and gene sequences can be searched by a variety of tools for certain properties. Sequence profiling tools can find restriction enzyme sites, open reading frames in nucleotide sequences, and predict secondary structures. Phylogenetic trees can be constructed and evolutionary hypotheses developed using special software like ClustalW regarding the ancestry of modern organisms and the genes they express. The field of bioinformatics is now indispensable for the analysis of genes and proteins.


title: Protein

text: A more complex computational problem is the prediction of intermolecular interactions, such as in molecular docking, protein folding, protein–protein interaction and chemical reactivity. Mathematical models to simulate these dynamical processes involve molecular mechanics, in particular, molecular dynamics. In this regard, "in silico" simulations discovered the folding of small α-helical protein domains such as the villin headpiece, the HIV accessory protein and hybrid methods combining standard molecular dynamics with quantum mechanical mathematics have explored the electronic states of rhodopsins.


title: Protein

text: Beyond classical molecular dynamics, quantum dynamics methods allow the simulation of proteins in atomistic detail with an accurate description of quantum mechanical effects. Examples include the multi-layer multi-configuration time-dependent Hartree (MCTDH) method and the hierarchical equations of motion (HEOM) approach, which have been applied to plant cryptochromes and bacteria light-harvesting complexes, respectively. Both quantum and classical mechanical simulations of biological-scale systems are extremely computationally demanding, so distributed computing initiatives (for example, the Folding@home project) facilitate the molecular modeling by exploiting advances in GPU parallel processing and Monte Carlo techniques.


title: Protein

text: The total nitrogen content of organic matter is mainly formed by the amino groups in proteins. The Total Kjeldahl Nitrogen (TKN) is a measure of nitrogen widely used in the analysis of (waste) water, soil, food, feed and organic matter in general. As the name suggests, the Kjeldahl method is applied. More sensitive methods are available.


title: Protein

text: Most microorganisms and plants can biosynthesize all 20 standard amino acids, while animals (including humans) must obtain some of the amino acids from the diet. The amino acids that an organism cannot synthesize on its own are referred to as essential amino acids. Key enzymes that synthesize certain amino acids are not present in animals—such as aspartokinase, which catalyses the first step in the synthesis of lysine, methionine, and threonine from aspartate. If amino acids are present in the environment, microorganisms can conserve energy by taking up the amino acids from their surroundings and downregulating their biosynthetic pathways.


title: Protein

text: In animals, amino acids are obtained through the consumption of foods containing protein. Ingested proteins are then broken down into amino acids through digestion, which typically involves denaturation of the protein through exposure to acid and hydrolysis by enzymes called proteases. Some ingested amino acids are used for protein biosynthesis, while others are converted to glucose through gluconeogenesis, or fed into the citric acid cycle. This use of protein as a fuel is particularly important under starvation conditions as it allows the body's own proteins to be used to support life, particularly those found in muscle.


title: Protein

text: In animals such as dogs and cats, protein maintains the health and quality of the skin by promoting hair follicle growth and keratinization, and thus reducing the likelihood of skin problems producing malodours. Poor-quality proteins also have a role regarding gastrointestinal health, increasing the potential for flatulence and odorous compounds in dogs because when proteins reach the colon in an undigested state, they are fermented producing hydrogen sulfide gas, indole, and skatole. Dogs and cats digest animal proteins better than those from plants, but products of low-quality animal origin are poorly digested, including skin, feathers, and connective tissue.


title: Light-emitting diode

text: A light-emitting diode (LED) is a semiconductor device that emits light when current flows through it. Electrons in the semiconductor recombine with electron holes, releasing energy in the form of photons. The color of the light (corresponding to the energy of the photons) is determined by the energy required for electrons to cross the band gap of the semiconductor. White light is obtained by using multiple semiconductors or a layer of light-emitting phosphor on the semiconductor device.


title: Light-emitting diode

text: Appearing as practical electronic components in 1962, the earliest LEDs emitted low-intensity infrared (IR) light. Infrared LEDs are used in remote-control circuits, such as those used with a wide variety of consumer electronics. The first visible-light LEDs were of low intensity and limited to red. Early LEDs were often used as indicator lamps, replacing small incandescent bulbs, and in seven-segment displays. Later developments produced LEDs available in visible, ultraviolet (UV), and infrared wavelengths, with high, low, or intermediate light output, for instance white LEDs suitable for room and outdoor area lighting. LEDs have also given rise to new types of displays and sensors, while their high switching rates are useful in advanced communications technology with applications as diverse as aviation lighting, fairy lights, automotive headlamps, advertising, general lighting, traffic signals, camera flashes, lighted wallpaper, horticultural grow lights, and medical devices.


title: Light-emitting diode

text: LEDs have many advantages over incandescent light sources, including lower power consumption, longer lifetime, improved physical robustness, smaller size, and faster switching. In exchange for these generally favorable attributes, disadvantages of LEDs include electrical limitations to low voltage and generally to DC (not AC) power, inability to provide steady illumination from a pulsing DC or an AC electrical supply source, and lesser maximum operating temperature and storage temperature. In contrast to LEDs, incandescent lamps can be made to intrinsically run at virtually any supply voltage, can utilize either AC or DC current interchangeably, and will provide steady illumination when powered by AC or pulsing DC even at a frequency as low as 50 Hz. LEDs usually need electronic support components to function, while an incandescent bulb can and usually does operate directly from an unregulated DC or AC power source.


title: Light-emitting diode

text: Electroluminescence as a phenomenon was discovered in 1907 by the English experimenter H. J. Round of Marconi Labs, using a crystal of silicon carbide and a cat's-whisker detector. Russian inventor Oleg Losev reported creation of the first LED in 1927. His research was distributed in Soviet, German and British scientific journals, but no practical use was made of the discovery for several decades.


title: Light-emitting diode

text: In 1936, Georges Destriau observed that electroluminescence could be produced when zinc sulphide (ZnS) powder is suspended in an insulator and an alternating electrical field is applied to it. In his publications, Destriau often referred to luminescence as Losev-Light. Destriau worked in the laboratories of Madame Marie Curie, also an early pioneer in the field of luminescence with research on radium.


title: Light-emitting diode

text: Hungarian Zoltán Bay together with György Szigeti pre-empted LED lighting in Hungary in 1939 by patenting a lighting device based on silicon carbide, with an option on boron carbide, that emitted white, yellowish white, or greenish white depending on impurities present.


title: Light-emitting diode

text: Kurt Lehovec, Carl Accardo, and Edward Jamgochian explained these first LEDs in 1951 using an apparatus employing SiC crystals with a current source of a battery or a pulse generator and with a comparison to a variant, pure, crystal in 1953.


title: Light-emitting diode

text: Rubin Braunstein of the Radio Corporation of America reported on infrared emission from gallium arsenide (GaAs) and other semiconductor alloys in 1955. Braunstein observed infrared emission generated by simple diode structures using gallium antimonide (GaSb), GaAs, indium phosphide (InP), and silicon-germanium (SiGe) alloys at room temperature and at 77 kelvins.


title: Light-emitting diode

text: In 1957, Braunstein further demonstrated that the rudimentary devices could be used for non-radio communication across a short distance. As noted by Kroemer Braunstein "…had set up a simple optical communications link: Music emerging from a record player was used via suitable electronics to modulate the forward current of a GaAs diode. The emitted light was detected by a PbS diode some distance away. This signal was fed into an audio amplifier and played back by a loudspeaker. Intercepting the beam stopped the music. We had a great deal of fun playing with this setup." This setup presaged the use of LEDs for optical communication applications.


title: Light-emitting diode

text: In September 1961, while working at Texas Instruments in Dallas, Texas, James R. Biard and Gary Pittman discovered near-infrared (900 nm) light emission from a tunnel diode they had constructed on a GaAs substrate. By October 1961, they had demonstrated efficient light emission and signal coupling between a GaAs p-n junction light emitter and an electrically isolated semiconductor photodetector. On August 8, 1962, Biard and Pittman filed a patent titled "Semiconductor Radiant Diode" based on their findings, which described a zinc-diffused p–n junction LED with a spaced cathode contact to allow for efficient emission of infrared light under forward bias. After establishing the priority of their work based on engineering notebooks predating submissions from G.E. Labs, RCA Research Labs, IBM Research Labs, Bell Labs, and Lincoln Lab at MIT, the U.S. patent office issued the two inventors the patent for the GaAs infrared light-emitting diode (U.S. Patent US3293513), the first practical LED. Immediately after filing the patent, Texas Instruments (TI) began a project to manufacture infrared diodes. In October 1962, TI announced the first commercial LED product (the SNX-100), which employed a pure GaAs crystal to emit an 890 nm light output. In October 1963, TI announced the first commercial hemispherical LED, the SNX-110.


title: Light-emitting diode

text: The first visible-spectrum (red) LED was demonstrated by J. W. Allen and R. J. Cherry in late 1961 at the SERL in Baldock, UK. This work was reported in the "Journal of Physics and Chemistry of Solids", Volume 23, Issue 5, May 1962, pages 509–511. Another early device was demonstrated by Nick Holonyak on October 9, 1962, while he was working for General Electric in Syracuse, New York. Holonyak and Bevacqua reported this LED in the journal "Applied Physics Letters" on December 1, 1962. M. George Craford, a former graduate student of Holonyak, invented the first yellow LED and improved the brightness of red and red-orange LEDs by a factor of ten in 1972. In 1976, T. P. Pearsall designed the first high-brightness, high-efficiency LEDs for optical fiber telecommunications by inventing new semiconductor materials specifically adapted to optical fiber transmission wavelengths.


title: Light-emitting diode

text: The first commercial visible-wavelength LEDs were commonly used as replacements for incandescent and neon indicator lamps, and in seven-segment displays, first in expensive equipment such as laboratory and electronics test equipment, then later in such appliances as calculators, TVs, radios, telephones, as well as watches (see list of signal uses).


title: Light-emitting diode

text: Until 1968, visible and infrared LEDs were extremely costly, in the order of US$200 per unit, and so had little practical use.


title: Light-emitting diode

text: Hewlett-Packard (HP) was engaged in research and development (R&D) on practical LEDs between 1962 and 1968, by a research team under Howard C. Borden, Gerald P. Pighini at HP Associates and HP Labs. During this time HP collaborated with Monsanto Company on developing the first usable LED products. The first usable LED products were HP's LED display and Monsanto's LED indicator lamp, both launched in 1968. Monsanto was the first organization to mass-produce visible LEDs, using GaAsP in 1968 to produce red LEDs suitable for indicators. Monsanto had previously offered to supply HP with GaAsP, but HP decided to grow its own GaAsP. In February 1969, Hewlett-Packard introduced the HP Model 5082-7000 Numeric Indicator, the first LED device to use integrated circuit (integrated LED circuit) technology. It was the first intelligent LED display, and was a revolution in digital display technology, replacing the Nixie tube and becoming the basis for later LED displays.


title: Light-emitting diode

text: In the 1970s, commercially successful LED devices at less than five cents each were produced by Fairchild Optoelectronics. These devices employed compound semiconductor chips fabricated with the planar process (developed by Jean Hoerni, ). The combination of planar processing for chip fabrication and innovative packaging methods enabled the team at Fairchild led by optoelectronics pioneer Thomas Brandt to achieve the needed cost reductions. LED producers continue to use these methods.


title: Light-emitting diode

text: The early red LEDs were bright enough only for use as indicators, as the light output was not enough to illuminate an area. Readouts in calculators were so small that plastic lenses were built over each digit to make them legible. Later, other colors became widely available and appeared in appliances and equipment.


title: Light-emitting diode

text: Early LEDs were packaged in metal cases similar to those of transistors, with a glass window or lens to let the light out. Modern indicator LEDs are packed in transparent molded plastic cases, tubular or rectangular in shape, and often tinted to match the device color. Infrared devices may be dyed, to block visible light. More complex packages have been adapted for efficient heat dissipation in high-power LEDs. Surface-mounted LEDs further reduce the package size. LEDs intended for use with fiber optics cables may be provided with an optical connector.


title: Light-emitting diode

text: The first blue-violet LED using magnesium-doped gallium nitride was made at Stanford University in 1972 by Herb Maruska and Wally Rhines, doctoral students in materials science and engineering. At the time Maruska was on leave from RCA Laboratories, where he collaborated with Jacques Pankove on related work. In 1971, the year after Maruska left for Stanford, his RCA colleagues Pankove and Ed Miller demonstrated the first blue electroluminescence from zinc-doped gallium nitride, though the subsequent device Pankove and Miller built, the first actual gallium nitride light-emitting diode, emitted green light. In 1974 the U.S. Patent Office awarded Maruska, Rhines and Stanford professor David Stevenson a patent for their work in 1972 (U.S. Patent US3819974 A). Today, magnesium-doping of gallium nitride remains the basis for all commercial blue LEDs and laser diodes. In the early 1970s, these devices were too dim for practical use, and research into gallium nitride devices slowed.


title: Light-emitting diode

text: In August 1989, Cree introduced the first commercially available blue LED based on the indirect bandgap semiconductor, silicon carbide (SiC). SiC LEDs had very low efficiency, no more than about 0.03%, but did emit in the blue portion of the visible light spectrum.


title: Light-emitting diode

text: In the late 1980s, key breakthroughs in GaN epitaxial growth and p-type doping ushered in the modern era of GaN-based optoelectronic devices. Building upon this foundation, Theodore Moustakas at Boston University patented a method for producing high-brightness blue LEDs using a new two-step process in 1991.


title: Light-emitting diode

text: Two years later, in 1993, high-brightness blue LEDs were demonstrated by Shuji Nakamura of Nichia Corporation using a gallium nitride growth process. In parallel, Isamu Akasaki and Hiroshi Amano of Nagoya University were working on developing the important GaN deposition on sapphire substrates and the demonstration of p-type doping of GaN. This new development revolutionized LED lighting, making high-power blue light sources practical, leading to the development of technologies like Blu-ray.


title: Light-emitting diode

text: Nakamura, Hiroshi Amano and Isamu Akasaki were awarded the Nobel Prize in Physics in 2014 for the invention of the blue LED. In 2015, a US court ruled that three companies had infringed Moustakas's prior patent, and ordered them to pay licensing fees of not less than US$13 million.


title: Light-emitting diode

text: In 1995, Alberto Barbieri at the Cardiff University Laboratory (GB) investigated the efficiency and reliability of high-brightness LEDs and demonstrated a "transparent contact" LED using indium tin oxide (ITO) on (AlGaInP/GaAs).


title: Light-emitting diode

text: In 2001 and 2002, processes for growing gallium nitride (GaN) LEDs on silicon were successfully demonstrated. In January 2012, Osram demonstrated high-power InGaN LEDs grown on silicon substrates commercially, and GaN-on-silicon LEDs are in production at Plessey Semiconductors. As of 2017, some manufacturers are using SiC as the substrate for LED production, but sapphire is more common, as it has the most similar properties to that of gallium nitride, reducing the need for patterning the sapphire wafer (patterned wafers are known as epi wafers). Samsung, the University of Cambridge, and Toshiba are performing research into GaN on Si LEDs. Toshiba has stopped research, possibly due to low yields. Some opt for epitaxy, which is difficult on silicon, while others, like the University of Cambridge, choose a multi-layer structure, in order to reduce (crystal) lattice mismatch and different thermal expansion ratios, in order to avoid cracking of the LED chip at high temperatures (e.g. during manufacturing), reduce heat generation and increase luminous efficiency. Sapphire substrate patterning can be carried out with nanoimprint lithography.


title: Light-emitting diode

text: GaN-on-Si is difficult but desirable since it takes advantage of existing semiconductor manufacturing infrastructure. It allows for the wafer-level packaging of LED dies resulting in extremely small LED packages.


title: Light-emitting diode

text: GaN is often deposited using Metalorganic vapour-phase epitaxy (MOCVD), and it also utilizes Lift-off.


title: Light-emitting diode

text: Even though white light can be created using individual red, green and blue LEDs, this results in poor color rendering, since only three narrow bands of wavelengths of light are being emitted. The attainment of high efficiency blue LEDs was quickly followed by the development of the first white LED. In this device a :Ce (known as "YAG" or Ce:YAG phosphor) cerium-doped phosphor coating produces yellow light through fluorescence. The combination of that yellow with remaining blue light appears white to the eye. Using different phosphors produces green and red light through fluorescence. The resulting mixture of red, green and blue is perceived as white light, with improved color rendering compared to wavelengths from the blue LED/YAG phosphor combination.


title: Light-emitting diode

text: The first white LEDs were expensive and inefficient. The light output then increased exponentially. The latest research and development has been propagated by Japanese manufacturers such as Panasonic, and Nichia, and by Korean and Chinese manufacturers such as Samsung, Solstice, Kingsun, Hoyol and others. This trend in increased output has been called Haitz's law after Roland Haitz.


title: Light-emitting diode

text: Light output and efficiency of blue and near-ultraviolet LEDs rose and the cost of reliable devices fell. This led to relatively high-power white-light LEDs for illumination, which are replacing incandescent and fluorescent lighting.


title: Light-emitting diode

text: Experimental white LEDs were demonstrated in 2014 to produce 303 lumens per watt of electricity (lm/W); some can last up to 100,000 hours. Commercially available LEDs have an efficiency of up to 223 lm/W as of 2018. A previous record of 135 lm/W was achieved by Nichia in 2010. Compared to incandescent bulbs, this is a huge increase in electrical efficiency, and even though LEDs are more expensive to purchase, overall lifetime cost is significantly cheaper than that of incandescent bulbs.


title: Light-emitting diode

text: The LED chip is encapsulated inside a small, plastic, white mold. It can be encapsulated using resin (polyurethane-based), silicone, or epoxy containing (powdered) Cerium-doped YAG phosphor. After allowing the solvents to evaporate, the LEDs are often tested, and placed on tapes for SMT placement equipment for use in LED light bulb production. Encapsulation is performed after probing, dicing, die transfer from wafer to package, and wire bonding or flip chip mounting, perhaps using Indium tin oxide, a transparent electrical conductor. In this case, the bond wire(s) are attached to the ITO film that has been deposited in the LEDs.


title: Light-emitting diode

text: Some "remote phosphor" LED light bulbs use a single plastic cover with YAG phosphor for several blue LEDs, instead of using phosphor coatings on single-chip white LEDs.


title: Light-emitting diode

text: The temperature of the phosphor during operation and how it is applied limits the size of an LED die. Wafer-level packaged white LEDs allow for extremely small LEDs.


title: Light-emitting diode

text: In a light emitting diode, the recombination of electrons and electron holes in a semiconductor produces light (be it infrared, visible or UV), a process called "electroluminescence". The wavelength of the light depends on the energy band gap of the semiconductors used. Since these materials have a high index of refraction, design features of the devices such as special optical coatings and die shape are required to efficiently emit light.


title: Light-emitting diode

text: Unlike a laser, the light emitted from an LED is neither spectrally coherent nor even highly monochromatic. Its spectrum is sufficiently narrow that it appears to the human eye as a pure (saturated) color. Also unlike most lasers, its radiation is not spatially coherent, so it cannot approach the very high intensity characteristic of lasers.


title: Light-emitting diode

text: By selection of different semiconductor materials, single-color LEDs can be made that emit light in a narrow band of wavelengths from near-infrared through the visible spectrum and into the ultraviolet range. As the wavelengths become shorter, because of the larger band gap of these semiconductors, the operating voltage of the LED increases.


title: Light-emitting diode

text: Blue LEDs have an active region consisting of one or more InGaN quantum wells sandwiched between thicker layers of GaN, called cladding layers. By varying the relative In/Ga fraction in the InGaN quantum wells, the light emission can in theory be varied from violet to amber.


title: Light-emitting diode

text: Aluminium gallium nitride (AlGaN) of varying Al/Ga fraction can be used to manufacture the cladding and quantum well layers for ultraviolet LEDs, but these devices have not yet reached the level of efficiency and technological maturity of InGaN/GaN blue/green devices. If unalloyed GaN is used in this case to form the active quantum well layers, the device emits near-ultraviolet light with a peak wavelength centred around 365 nm. Green LEDs manufactured from the InGaN/GaN system are far more efficient and brighter than green LEDs produced with non-nitride material systems, but practical devices still exhibit efficiency too low for high-brightness applications.


title: Light-emitting diode

text: With AlGaN and AlGaInN, even shorter wavelengths are achievable. Near-UV emitters at wavelengths around 360–395 nm are already cheap and often encountered, for example, as black light lamp replacements for inspection of anti-counterfeiting UV watermarks in documents and bank notes, and for UV curing. Substantially more expensive, shorter-wavelength diodes are commercially available for wavelengths down to 240 nm. As the photosensitivity of microorganisms approximately matches the absorption spectrum of DNA, with a peak at about 260 nm, UV LED emitting at 250–270 nm are expected in prospective disinfection and sterilization devices. Recent research has shown that commercially available UVA LEDs (365 nm) are already effective disinfection and sterilization devices.


title: Light-emitting diode

text: UV-C wavelengths were obtained in laboratories using aluminium nitride (210 nm), boron nitride (215 nm) and diamond (235 nm).


title: Light-emitting diode

text: There are two primary ways of producing white light-emitting diodes. One is to use individual LEDs that emit three primary colors—red, green and blue—and then mix all the colors to form white light. The other is to use a phosphor material to convert monochromatic light from a blue or UV LED to broad-spectrum white light, similar to a fluorescent lamp. The yellow phosphor is cerium-doped YAG crystals suspended in the package or coated on the LED. This YAG phosphor causes white LEDs to appear yellow when off, and the space between the crystals allow some blue light to pass through in LEDs with partial phosphor conversion. Alternatively, white LEDs may use other phosphors like manganese(IV)-doped potassium fluorosilicate (PFS) or other engineered phosphors. PFS assists in red light generation, and is used in conjunction with conventional Ce:YAG phosphor. In LEDs with PFS phosphor, some blue light passes through the phosphors, the Ce:YAG phosphor converts blue light to green and red (yellow) light, and the PFS phosphor converts blue light to red light. The color, emission spectrum or color temperature of white phosphor converted and other phosphor converted LEDs can be controlled by changing the concentration of several phosphors that form a phosphor blend used in an LED package.


title: Light-emitting diode

text: The 'whiteness' of the light produced is engineered to suit the human eye. Because of metamerism, it is possible to have quite different spectra that appear white. The appearance of objects illuminated by that light may vary as the spectrum varies. This is the issue of color rendition, quite separate from color temperature. An orange or cyan object could appear with the wrong color and much darker as the LED or phosphor does not emit the wavelength it reflects. The best color rendition LEDs use a mix of phosphors, resulting in less efficiency and better color rendering.


title: Light-emitting diode

text: Mixing red, green, and blue sources to produce white light needs electronic circuits to control the blending of the colors. Since LEDs have slightly different emission patterns, the color balance may change depending on the angle of view, even if the RGB sources are in a single package, so RGB diodes are seldom used to produce white lighting. Nonetheless, this method has many applications because of the flexibility of mixing different colors, and in principle, this mechanism also has higher quantum efficiency in producing white light.


title: Light-emitting diode

text: There are several types of multicolor white LEDs: di-, tri-, and tetrachromatic white LEDs. Several key factors that play among these different methods include color stability, color rendering capability, and luminous efficacy. Often, higher efficiency means lower color rendering, presenting a trade-off between the luminous efficacy and color rendering. For example, the dichromatic white LEDs have the best luminous efficacy (120 lm/W), but the lowest color rendering capability. Although tetrachromatic white LEDs have excellent color rendering capability, they often have poor luminous efficacy. Trichromatic white LEDs are in between, having both good luminous efficacy (>70 lm/W) and fair color rendering capability.


title: Light-emitting diode

text: One of the challenges is the development of more efficient green LEDs. The theoretical maximum for green LEDs is 683 lumens per watt but as of 2010 few green LEDs exceed even 100 lumens per watt. The blue and red LEDs approach their theoretical limits.


title: Light-emitting diode

text: Multicolor LEDs offer a means to form light of different colors. Most perceivable colors can be formed by mixing different amounts of three primary colors. This allows precise dynamic color control. Their emission power decays exponentially with rising temperature,


title: Light-emitting diode

text: resulting in a substantial change in color stability. Such problems inhibit industrial use. Multicolor LEDs without phosphors cannot provide good color rendering because each LED is a narrowband source. LEDs without phosphor, while a poorer solution for general lighting, are the best solution for displays, either backlight of LCD, or direct LED based pixels.


title: Light-emitting diode

text: Dimming a multicolor LED source to match the characteristics of incandescent lamps is difficult because manufacturing variations, age, and temperature change the actual color value output. To emulate the appearance of dimming incandescent lamps may require a feedback system with color sensor to actively monitor and control the color.


title: Light-emitting diode

text: This method involves coating LEDs of one color (mostly blue LEDs made of InGaN) with phosphors of different colors to form white light; the resultant LEDs are called phosphor-based or phosphor-converted white LEDs (pcLEDs). A fraction of the blue light undergoes the Stokes shift, which transforms it from shorter wavelengths to longer. Depending on the original LED's color, various color phosphors are used. Using several phosphor layers of distinct colors broadens the emitted spectrum, effectively raising the color rendering index (CRI).


title: Light-emitting diode

text: Phosphor-based LEDs have efficiency losses due to heat loss from the Stokes shift and also other phosphor-related issues. Their luminous efficacies compared to normal LEDs depend on the spectral distribution of the resultant light output and the original wavelength of the LED itself. For example, the luminous efficacy of a typical YAG yellow phosphor based white LED ranges from 3 to 5 times the luminous efficacy of the original blue LED because of the human eye's greater sensitivity to yellow than to blue (as modeled in the luminosity function). Due to the simplicity of manufacturing, the phosphor method is still the most popular method for making high-intensity white LEDs. The design and production of a light source or light fixture using a monochrome emitter with phosphor conversion is simpler and cheaper than a complex RGB system, and the majority of high-intensity white LEDs presently on the market are manufactured using phosphor light conversion.


title: Light-emitting diode

text: Among the challenges being faced to improve the efficiency of LED-based white light sources is the development of more efficient phosphors. As of 2010, the most efficient yellow phosphor is still the YAG phosphor, with less than 10% Stokes shift loss. Losses attributable to internal optical losses due to re-absorption in the LED chip and in the LED packaging itself account typically for another 10% to 30% of efficiency loss. Currently, in the area of phosphor LED development, much effort is being spent on optimizing these devices to higher light output and higher operation temperatures. For instance, the efficiency can be raised by adapting better package design or by using a more suitable type of phosphor. Conformal coating process is frequently used to address the issue of varying phosphor thickness.


title: Light-emitting diode

text: Some phosphor-based white LEDs encapsulate InGaN blue LEDs inside phosphor-coated epoxy. Alternatively, the LED might be paired with a remote phosphor, a preformed polycarbonate piece coated with the phosphor material. Remote phosphors provide more diffuse light, which is desirable for many applications. Remote phosphor designs are also more tolerant of variations in the LED emissions spectrum. A common yellow phosphor material is cerium-doped yttrium aluminium garnet (Ce:YAG).


title: Light-emitting diode

text: White LEDs can also be made by coating near-ultraviolet (NUV) LEDs with a mixture of high-efficiency europium-based phosphors that emit red and blue, plus copper and aluminium-doped zinc sulfide (ZnS:Cu, Al) that emits green. This is a method analogous to the way fluorescent lamps work. This method is less efficient than blue LEDs with YAG:Ce phosphor, as the Stokes shift is larger, so more energy is converted to heat, but yields light with better spectral characteristics, which render color better. Due to the higher radiative output of the ultraviolet LEDs than of the blue ones, both methods offer comparable brightness. A concern is that UV light may leak from a malfunctioning light source and cause harm to human eyes or skin.


title: Light-emitting diode

text: Another method used to produce experimental white light LEDs used no phosphors at all and was based on homoepitaxially grown zinc selenide (ZnSe) on a ZnSe substrate that simultaneously emitted blue light from its active region and yellow light from the substrate.


title: Light-emitting diode

text: A new style of wafers composed of gallium-nitride-on-silicon (GaN-on-Si) is being used to produce white LEDs using 200-mm silicon wafers. This avoids the typical costly sapphire substrate in relatively small 100- or 150-mm wafer sizes. The sapphire apparatus must be coupled with a mirror-like collector to reflect light that would otherwise be wasted. It was predicted that since 2020, 40% of all GaN LEDs are made with GaN-on-Si. Manufacturing large sapphire material is difficult, while large silicon material is cheaper and more abundant. LED companies shifting from using sapphire to silicon should be a minimal investment.


title: Light-emitting diode

text: In an organic light-emitting diode (OLED), the electroluminescent material composing the emissive layer of the diode is an organic compound. The organic material is electrically conductive due to the delocalization of pi electrons caused by conjugation over all or part of the molecule, and the material therefore functions as an organic semiconductor. The organic materials can be small organic molecules in a crystalline phase, or polymers.


title: Light-emitting diode

text: The potential advantages of OLEDs include thin, low-cost displays with a low driving voltage, wide viewing angle, and high contrast and color gamut. Polymer LEDs have the added benefit of printable and flexible displays. OLEDs have been used to make visual displays for portable electronic devices such as cellphones, digital cameras, lighting and televisions.


title: Light-emitting diode

text: LEDs are made in different packages for different applications. A single or a few LED junctions may be packed in one miniature device for use as an indicator or pilot lamp. An LED array may include controlling circuits within the same package, which may range from a simple resistor, blinking or color changing control, or an addressable controller for RGB devices. Higher-powered white-emitting devices will be mounted on heat sinks and will be used for illumination. Alphanumeric displays in dot matrix or bar formats are widely available. Special packages permit connection of LEDs to optical fibers for high-speed data communication links.


title: Light-emitting diode

text: These are mostly single-die LEDs used as indicators, and they come in various sizes from 2 mm to 8 mm, through-hole and surface mount packages. Typical current ratings range from around 1 mA to above 20 mA. Multiple LED dies attached to a flexible backing tape form an LED strip light.


title: Light-emitting diode

text: Common package shapes include round, with a domed or flat top, rectangular with a flat top (as used in bar-graph displays), and triangular or square with a flat top. The encapsulation may also be clear or tinted to improve contrast and viewing angle. Infrared devices may have a black tint to block visible light while passing infrared radiation.


title: Light-emitting diode

text: 5 V and 12 V LEDs are ordinary miniature LEDs that have a series resistor for direct connection to a 5V or 12V supply.


title: Light-emitting diode

text: High-power LEDs (HP-LEDs) or high-output LEDs (HO-LEDs) can be driven at currents from hundreds of mA to more than an ampere, compared with the tens of mA for other LEDs. Some can emit over a thousand lumens. LED power densities up to 300 W/cm have been achieved. Since overheating is destructive, the HP-LEDs must be mounted on a heat sink to allow for heat dissipation. If the heat from an HP-LED is not removed, the device fails in seconds. One HP-LED can often replace an incandescent bulb in a flashlight, or be set in an array to form a powerful LED lamp.


title: Light-emitting diode

text: Some well-known HP-LEDs in this category are the Nichia 19 series, Lumileds Rebel Led, Osram Opto Semiconductors Golden Dragon, and Cree X-lamp. As of September 2009, some HP-LEDs manufactured by Cree now exceed 105 lm/W.


title: Light-emitting diode

text: Examples for Haitz's law—which predicts an exponential rise in light output and efficacy of LEDs over time—are the CREE XP-G series LED, which achieved 105lm/W in 2009 and the Nichia 19 series with a typical efficacy of 140lm/W, released in 2010.


title: Light-emitting diode

text: LEDs developed by Seoul Semiconductor can operate on AC power without a DC converter. For each half-cycle, part of the LED emits light and part is dark, and this is reversed during the next half-cycle. The efficiency of this type of HP-LED is typically 40lm/W. A large number of LED elements in series may be able to operate directly from line voltage. In 2009, Seoul Semiconductor released a high DC voltage LED, named 'Acrich MJT', capable of being driven from AC power with a simple controlling circuit. The low-power dissipation of these LEDs affords them more flexibility than the original AC LED design.


title: Light-emitting diode

text: Flashing LEDs are used as attention seeking indicators without requiring external electronics. Flashing LEDs resemble standard LEDs but they contain an integrated voltage regulator and a multivibrator circuit that causes the LED to flash with a typical period of one second. In diffused lens LEDs, this circuit is visible as a small black dot. Most flashing LEDs emit light of one color, but more sophisticated devices can flash between multiple colors and even fade through a color sequence using RGB color mixing.


title: Light-emitting diode

text: Bi-color LEDs contain two different LED emitters in one case. There are two types of these. One type consists of two dies connected to the same two leads antiparallel to each other. Current flow in one direction emits one color, and current in the opposite direction emits the other color. The other type consists of two dies with separate leads for both dies and another lead for common anode or cathode so that they can be controlled independently. The most common bi-color combination is red/traditional green. Others include amber/traditional green, red/pure green, red/blue, and blue/pure green.


title: Light-emitting diode

text: Tri-color LEDs contain three different LED emitters in one case. Each emitter is connected to a separate lead so they can be controlled independently. A four-lead arrangement is typical with one common lead (anode or cathode) and an additional lead for each color. Others have only two leads (positive and negative) and have a built-in electronic controller.


title: Light-emitting diode

text: RGB LEDs consist of one red, one green, and one blue LED. By independently adjusting each of the three, RGB LEDs are capable of producing a wide color gamut. Unlike dedicated-color LEDs, these do not produce pure wavelengths. Modules may not be optimized for smooth color mixing.


title: Light-emitting diode

text: Decorative-multicolor LEDs incorporate several emitters of different colors supplied by only two lead-out wires. Colors are switched internally by varying the supply voltage.


title: Light-emitting diode

text: Alphanumeric LEDs are available in seven-segment, starburst, and dot-matrix format. Seven-segment displays handle all numbers and a limited set of letters. Starburst displays can display all letters. Dot-matrix displays typically use 5×7 pixels per character. Seven-segment LED displays were in widespread use in the 1970s and 1980s, but rising use of liquid crystal displays, with their lower power needs and greater display flexibility, has reduced the popularity of numeric and alphanumeric LED displays.


title: Light-emitting diode

text: Digital RGB addressable LEDs contain their own "smart" control electronics. In addition to power and ground, these provide connections for data-in, data-out, clock and sometimes a strobe signal. These are connected in a daisy chain. Data sent to the first LED of the chain can control the brightness and color of each LED independently of the others. They are used where a combination of maximum control and minimum visible electronics are needed such as strings for Christmas and LED matrices. Some even have refresh rates in the kHz range, allowing for basic video applications. These devices are known by their part number (WS2812 being common) or a brand name such as NeoPixel.


title: Light-emitting diode

text: An LED filament consists of multiple LED chips connected in series on a common longitudinal substrate that forms a thin rod reminiscent of a traditional incandescent filament. These are being used as a low-cost decorative alternative for traditional light bulbs that are being phased out in many countries. The filaments use a rather high voltage, allowing them to work efficiently with mains voltages. Often a simple rectifier and capacitive current limiting are employed to create a low-cost replacement for a traditional light bulb without the complexity of the low voltage, high current converter that single die LEDs need. Usually, they are packaged in bulb similar to the lamps they were designed to replace, and filled with inert gas at slightly lower than ambient pressure to remove heat efficiently and prevent corrosion.


title: Light-emitting diode

text: Surface-mounted LEDs are frequently produced in chip on board (COB) arrays, allowing better heat dissipation than with a single LED of comparable luminous output. The LEDs can be arranged around a cylinder, and are called "corn cob lights" because of the rows of yellow LEDs.


title: Light-emitting diode

text: The current in an LED or other diodes rises exponentially with the applied voltage (see Shockley diode equation), so a small change in voltage can cause a large change in current. Current through the LED must be regulated by an external circuit such as a constant current source to prevent damage. Since most common power supplies are (nearly) constant-voltage sources, LED fixtures must include a power converter, or at least a current-limiting resistor. In some applications, the internal resistance of small batteries is sufficient to keep current within the LED rating.


title: Light-emitting diode

text: Unlike a traditional incandescent lamp, an LED will light only when voltage is applied in the forward direction of the diode. No current flows and no light is emitted if voltage is applied in the reverse direction. If the reverse voltage exceeds the breakdown voltage, a large current flows and the LED will be damaged. If the reverse current is sufficiently limited to avoid damage, the reverse-conducting LED is a useful noise diode.


title: Light-emitting diode

text: Certain blue LEDs and cool-white LEDs can exceed safe limits of the so-called blue-light hazard as defined in eye safety specifications such as "ANSI/IESNA RP-27.1–05: Recommended Practice for Photobiological Safety for Lamp and Lamp Systems". One study showed no evidence of a risk in normal use at domestic illuminance, and that caution is only needed for particular occupational situations or for specific populations. In 2006, the International Electrotechnical Commission published "IEC 62471 Photobiological safety of lamps and lamp systems", replacing the application of early laser-oriented standards for classification of LED sources.


title: Light-emitting diode

text: While LEDs have the advantage over fluorescent lamps, in that they do not contain mercury, they may contain other hazardous metals such as lead and arsenic.


title: Light-emitting diode

text: In 2016 the American Medical Association (AMA) issued a statement concerning the possible adverse influence of blueish street lighting on the sleep-wake cycle of city-dwellers. Industry critics claim exposure levels are not high enough to have a noticeable effect.