in order to utilize pituitary luteinizing hormone ( lh ) secretion as a bioassay of hypothalamic gnrh release , juvenile males were castrated and the responsiveness of their gonadotrophs to gnrh was enhanced prior to the study with a chronic intermittent intravenous infusion of the synthetic decapeptide ( 0.1 microgram/min for 3 min every hour ) .
C0020663
hypothalamic
C0023610
gnrh