in order to determine the relative activity of pyrimidine nucleoside-2',3'-cyclophosphates as donors and nucleosides as acceptors of phosphate in the reaction of the internucleotide bond formation catalyzed by rnaase a ( ec 3.4.1.22 ) , a comparative synthesis of dinucleoside monophosphates upu , upc , cpu and cpc at three different enzyme concentrations ( 20 , 40 and 70 mkg/ml ) and two temperatures ( 0 degrees and -15 degrees ) was carried out .
C0034287
pyrimidine
C0028621
nucleosides