the method aforementioned ( liu , w. and tsou , c.l . ( 1987 ) biochim . biophys . acta 916 , 455-464 ) for the study of the kinetics of irreversible modification of enzyme activity has been applied to the reactivation of guanidine-denatured ribonuclease a , by following the hydrolysis of cyclic cmp during refolding upon diluting a guanidine-denatured enzyme with a substrate-containing buffer .
C0035542
ribonuclease
C0020291
hydrolysis