to elute adsorbed enzymes from immobilized zn2+ three methods were compared : ( 1 ) removal of zn2+ with chelators such as edta , ( 2 ) introduction of ligands to compete with enzyme for zn2+ and ( 3 ) lowering ph to protonate sidechains in the enzyme that serve as ligands to zn2+ .
C0014444
enzymes
C0014442
enzyme