incubation of glomerular homogenates ( 200 micrograms protein ) with glomerular basement membrane ( gbm , 30-35 micrograms hydroxyproline ) at ph 7.5 for 36 h at 37 degrees c resulted in significant gbm degradation as measured by hydroxyproline release ( 40 +/- 6 % , n = 17 ) .
C0022663
glomerular
C0599297
glomerular