the addition of 1 % of glycine ( for y. pseudotuberculosis ) , 1-1.5 % of methionine in combination with growth stimulators has made it possible to obtain yersinia spheroplasts and protoplasts with the intact cytoplasmic membrane , thus permitting the isolation and purification of the cytoplasmic membrane fraction .
C0010834
cytoplasmic
C0007603
cytoplasmic membrane