when peripheral blood mononuclear cells from one healthy donor were activated in vitro by the t cell mitogen anti-cd3 monoclonal antibody , the maximal number of cells producing interleukin ( il ) 2 , il 6 or tumor necrosis factor ( tnf ) -alpha occurred 6 h after initiation of the cultures , while peak numbers of interferon-gamma , tnf-beta and a second wave of tnf-alpha-synthesizing cells were noted approximately 20 h later .
C1321301
peripheral blood
C0007634
cells