the following procedure for the detection of non-acetylated amino sugars in the plasma membrane was established : i ) derivatization of free amino groups with dansyl-chloride , ii ) hydrolysis with 3 m hcl ( for 4 h at 105 degrees c ) to liberate the dansylated carbohydrate moieties from the plasma membrane , iii ) purification of the dansylated amino sugars by paper chromatography and subsequent analysis by thin-layer chromatography .
C0007004
carbohydrate
C0002531
amino sugars