extracts from ( 1 ) 300 day 6 rabbit blastocysts , ( 2 ) 300 day 2 unfertilized ova , and ( 3 ) uteri from the nonpregnant does were analyzed in the following assays in order to determine the presence of a chorionic gonadotropin : ( 1 ) in vitro bioassay for testosterone production by decapsulated rat testes , ( 2 ) in vitro bioassay for progesterone production by porcine granulosa cells , ( 3 ) in vitro determination of adenylyl cyclase-stimulating activity in rabbit graafian follicles , ( 4 ) gel filtration in a sephadex g-150 column and assay of the elutions in a radioimmunoassay ( ria ) specific for the beta-subunit of human chorionic gonadotropin ( hcg ) , ( 5 ) parallelism with hcg standard preparations in beta-hcg ria , and ( 6 ) concanavalin a-column chromatography .
C0018062
hcg
C0106132
beta-hcg