for the transformation of the yeast species kluyveromyces fragilis , we have constructed a vector containing a bacterial kanamycin resistance ( kmr ) gene , the trp1 gene of saccharomyces cerevisiae , and an autonomously replicating sequence of kluyveromyces lactis called kars2 . by utilizing the method based on treatment by alkali cations and with the kmr gene as the selective marker , a wild-type strain of k. fragilis was transformed to resistance against the antibiotic g418 . in the transformed cell the plasmid replicates autonomously .
C0242961
autonomously replicating sequence
C0007634
cell