the 70 % ethanol fixation for trf-r and pcna and the 95 % acetone fixation for ki-67 plus permeabilization ( with 0.1 % and 1 % triton x100 , respectively , for the surface and the nuclear antigens ) produced cell suspensions with negligible cell clumping , high-quality dna profiles , and bright specific immunofluorescent staining .
C0072108
pcna
C0069115
nuclear antigens