camk- ( 281-289 ) was a good substrate ( km = 11 microm ; vmax = 3.15 mumol/min/mg ) for the proteolyzed kinase whereas phosphorylation of camk- ( 281-309 ) showed nonlinear michaelis-menton kinetics , with maximal phosphorylation ( 0.1 mumol/min/mg ) at 20 microm and decreased phosphorylation at higher concentrations .
C0031727
kinase
C0031715
phosphorylation