the active adult muscle extracts are fractionated by deae-cellulose column chromatography and transferrin is identified as the active component based on the following findings : ( 1 ) the active fractions are shown to contain an 80k protein that comigrates with chicken transferrin on sds-page , ( 2 ) the active extract from the anterior latissimus dorsi completely replaced embryo extract in the culture medium and supported normal myogenesis , ( 3 ) the active extract requires iron for its ability to support myogenesis , ( 4 ) the peptide map of the 80k protein is identical to a peptide map of transferrin .
C0026845
muscle
C0596997
myogenesis