because of the heterogeneity of this fraction , due to the contamination of the 200 mmicro granules with mitochondria and other cellular debris , the active layer and the resuspended active pellet , obtained by centrifuging this layer first at 17,000 g then diluting the supernatant and centrifuging at 30,000 g for 1 hour , were filtered through millipore ha paper with a pore size of 0.45 micro .
C0026237
mitochondria
C0007634
cellular