using fab antibody fragments directed against protein crm 45 ( non-toxic cross-reacting material serologically-related to diphtheria toxin mutant protein , the product of a non-sens mutation in the diphtheria toxin gene ) or the b 45 domain of this molecule ( the remaining portion of the toxin b fragment present in protein crm 45 ) . , we have shown that the diphtheria toxin polyphosphate-binding site ( p site ) [ lory and collier ( 1980 ) proc . natl acad . sci . usa , 1 , 267-271 ] , evidenced by the ability of the toxin to bind atp , is affected upon incubation of this protein with the fab fragment of anti-protein ( crm 45 ) igg but not with the fab fragment of anti-polypeptide ( b 45 ) igg .
C1564139
mutant
C0033684
protein