for the assay of total dolichyl phosphate ( free and chemically bound ) , it was found that when rat liver is first saponified and then extracted with ethyl ether , the amount of dolichyl phosphate present in the ether extract is significantly greater than the sum of the amounts found in extracts derived by treating the tissue first with chloroform/methanol ( 2/1 ) and then with chloroform/ethanol/water ( 10/10/3 ) .
C0001963
methanol
C0001962
ethanol