inhibition experiments with substrate analogues demonstrated that ( i ) the major binding determinant of the glycosides was the aglycone moiety , ( ii ) an anionic side chain of the enzyme ( probably a carboxy group ) interacted with the glycosidic linkages and ( iii ) the properties of the aglycone significantly influenced the binding of the carbohydrate moiety .
C0021966
i
C0003075
anionic