the alpha-galactosidase was highly purified by ammonium sulfate fractionation , gel filtration on a sephadex g-100 column , ionexchange chromatography on deae-cellulose , and cm-cellulose columns , and heat treatment ( 60 degrees c , 15 min ) in the presence of 0.2 m d-galactose .
C0011050
deae-cellulose
C0007648
cellulose