estrogen receptors covalently labeled with the estrogen affinity label [ 3h ] ketononestrol aziridine ( kna ) or with the antiestrogen affinity label [ 3h ] tamoxifen aziridine ( taz ) were subjected to limited proteolysis with trypsin , alpha-chymotrypsin , and staphylococcus aureus v8 protease and then analyzed on 10-20 % sodium dodecyl sulfate-polyacrylamide gradient gels followed by fluorography .
C0597304
proteolysis
C0030946
protease