we have used immunofluorescence in parallel with transmission and scanning electron microscopy to characterize the unusual cytoplasmic and nucleolar accumulation of simian virus 40 ( sv40 ) virion protein ( c antigen ) at restrictive temperatures ( 39 to 41 c ) in monkey cells infected with a temperature-sensitive mutant of sv40 defective in virion assembly , tsb11 .
C0010834
cytoplasmic
C0007634
cells