we have used a stopped-flow apparatus to reinvestigate reports , based on the observation of `` '' burst '' '' kinetics , of an intermediate prior to the acyl-enzyme complex in hydrolysis reactions of anilides catalyzed by trypsin and elastase [ m. w. hunkapiller , m. d. forgac and j. h. richards ( 1976 ) biochemistry 15 , 5581-5588 ; d. d. petkov ( 1978 ) biochim . biophys . acta , 523 , 538-541 ; a. l. fink and p. meehan ( 1979 ) proc . natl acad . sci . usa , 76 , 1566-1569 ; p. compton and a. l. fink ( 1980 ) biochem . biophys . res . commun . 93 , 427-431 ] .
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