phosphocellulose paper binding assays on sonicates of the e. coli strain carrying the cloned kanamycin determinant demonstrated significant activity against kanamycin , neomycin , and amikacin but not against butirosin , gentamicin , tobramycin , or lividomycin , suggesting that the enzyme is the product of a 3'-o-aminoglycoside phosphotransferase type of aminoglycoside resistance gene .
C0022487
kanamycin
C0002499
amikacin