to isolate highly purified lysosomal preparations , lysosomes were loaded with horseradish peroxidase ( 2-h cell uptake , 16-h chase ) and isolated by isopycnic centrifugation in linear percoll gradients , followed by a 3,3'-diaminobenzidine-induced density shift in sucrose gradients .
C0024369
lysosomes
C0007634
cell