pbp2* retained the penicillin-binding activity , was localized in the cytoplasm and was overproduced under the control of the lpp-lac promoter . this indicates that the removed hydrophobic segment is an uncleaved signal sequence required for translocation of pbp2 across the cytoplasmic membrane , and also suggests that the segment anchors the protein to the membrane .
C0010834
cytoplasm
C0007603
cytoplasmic