expression of the major intracellular serine protease ( isp-1 ) gene of bacillus subtilis was studied by using a translational fusion plasmid in which the isp promoter region was fused to the lacz gene . beta-galactosidase activity , used to measure transcription from the isp promoter , was produced immediately after the end of exponential growth , whereas intracellular protease activity was not detected until 4 h later .
C0017262
expression
C1519614
translational