by selective sequential solubilization of the components of nil8 cells and analysis of the resulting cytoskeletal preparations by western blot technique and indirect immunofluorescence , we have shown that the 70 kda protein is associated with microtubules in mitotic and interphase cells and comigrates with hsp70 on 2-dimensional gel electrophoretigrams .
C0010853
cytoskeletal
C0026046
microtubules