the functional characterization of the mal6 subcloned regions was determined by ( 1 ) analyzing biochemically the levels of mal-encoded proteins ( maltase [ alpha-d-glucosidase , e.c . 3.2.1.20 ] and maltose transport protein ) in cells transformed with various mal6 subclones , and ( 2 ) testing the ability of the subclones to complement the maltose fermentation defects of well characterized mal- mutants in the highly homologous mal1 locus .
C0007634
cells
C1708726
locus