the [ gamma-32p ] at [ phosphotransferase reaction is heat-labile , dependent upon mg2+ but not ca2+ or cyclic gmp , inhibited by a cyclic amp dependent protein kinase inhibitor , and directly related to the amount of cytosolic protein which provides the endogenous source of both the protein kinase enzyme , atp , cyclic amp and phosphorylatable protein substrate .
C0031727
phosphotransferase
C0033640
protein kinase