the cytotoxic cells were identified as natural killer ( nk ) cells since nk-sensitive cells ( g1-tc and yac-1 cell lines ) were lysed by d and dp spleen cells , yac-1 cells competed for the lysis of rin islet cells by d spleen cells , lysis of rin cells was increased by using d spleen cells from the low density fraction ( large lymphocytes/monocytes ) of a percoll density gradient , and incubation of d spleen cells with an antiserum to nk cells ( anti-asialo gm1 serum ) and complement decreased monoclonal antibody-defined subsets containing nk cells ( w3/13+ ox19- and ox8+ ) , and this was accompanied by similar decreases in cytotoxicity to yac-1 , rin , and wf islet cells .
C0229671
serum
C0009498
complement