more than 95 % of the 35s-labeled sulfated glycoconjugates were extracted from the tissue with 4 m guanidine hcl , 50 mm sodium acetate , ph 6.0 , and 0.5 % triton x-100 , and separated clearly on deae-sephacel into three major fractions , i.e . sulfated glycoprotein ( 11 % of the total radioactivity ) , proteoheparan sulfate ( 33 % ) , and proteochondroitin sulfate ( 38 % ) fractions .
C0017906
glycoconjugates
C0017968
glycoprotein