at twenty four hr after the last injection , tissue slices of the midbrain raphe were prepared and the rate of serotonin biosynthesis was estimated by measuring formation of 5-hydroxytryptophan ( 5-htp ) from tryptophan during inhibition of aromatic l-amino acid decarboxylase using high-performance liquid chromatography with fluorescence detection .
C0000578
5-htp
C0041249
tryptophan