to determine whether replication is arrested at specific dna sites , forks were located on the sv40 genome by specifically 32p labelling 3 ' ends of nascent dna on purified replicating sv40 dna , isolating the longest 32p-dna chains , annealing them to sv40 dna and then digesting them with a restriction endonuclease that cut near the terminatin site .
C0598312
replication
C0012854
dna