subpopulations of human peripheral blood mononuclear lymphocytes , after depletion of b-cells and monocytes ( by sequential adherence to plastic and nylon wool columns ) were separated by percoll density gradient centrifugation and tested for their ability to proliferate in response to various mitogens , recall antigens , and alloantigens , and to develop cytolytic reactivity in vitro .
C0032167
plastic
C0028736
nylon