by using correlative light ( lm ) and scanning electron microscopy ( sem ) , it was shown that the effects of both the ultraviolet light ( u.v . ) and hot ssc treatment steps of the harlequin staining procedure were necessary to obtain sister-chromatid differentiation ( scd ) or replication banding . u.v . treatment alone resulted in dark giemsa staining of both chromatids with sem morphology of short compact protuberances and an overall flattened smooth appearance in both the unsubstituted and brdurd-substituted chromatids , a morphology essentially similar to that of untreated chromosomes .
C1519334
sister-chromatid
C0008633
chromosomes