the assay is based on the h2o2-dependent oxidation of homovanillic acid ( 3-methoxy-4-hydroxyphenylacetic acid , hva ) to a highly fluorescent dimer ( 2,2'-dihydroxy-3,3'-dimethoxydiphenyl-5,5'-diacetic acid ) which is mediated by horse-radish peroxidase .
C0030011
oxidation
C0027021
peroxidase