the chloramphenicol resistance determinant of a plasmid integrated at the subtilisin locus was mapped by pbs1 transduction and was found to be linked to glyb ( 83 % ) and argc ( 60 % ) , but not with metc or purb . the chromosomal locus containing the wild-type subtilisin allele was replaced with an in vitro-derived allele of the gene ( delta apr-684 ) that contained a 684-base-pair deletion .
C0008633
chromosomal
C1708726
locus