the method is based on selective extraction of gh from 15,000 g subcellular sediment of anterior pituitary gland with 130-150 mm nh4hco3 , 1 mm egta , ph 7.2-7.4 , at 2-6 degrees c for 60 min , purification of the extracted gh by ammonium sulfate fractionation , one-step ion-exchange column chromatography on deae-cellulose ( or cm-cellulose ) , and gel filtration on sephadex g-75 .
C0011050
deae-cellulose
C0007648
cellulose