the system ( i ) depends completely on low levels of exogenously furnished supercoiled oric plasmids , ( ii ) uses only those plasmids that contain the intact oric region of about 245 base pairs , ( iii ) initiates replication within or near the oric sequence and proceeds bidirectionally , ( iv ) proceeds linearly , after a 5-min lag , for 30-40 min to produce as much as a 40 % increase over the input dna , ( v ) depends on rna polymerase and gyrase as indicated by total inhibition by rifampicin and nalidixate , ( vi ) depends on replication proteins ( e.g. , dnab protein and single-stranded dna binding protein ) as judged by specific antibody inhibitions , ( vii ) operates independently from protein synthesis , and ( viii ) depends on dnaa activity , as suggested by the inactivity of enzyme fraction from each of two dnaa temperature-sensitive mutant strains , and complementation ( with a 15-fold overproduction of complementing activity ) by a fraction from a strain containing the dnaa gene cloned in a multicopy plasmid .
C0012854
dna
C0598312
replication