pituitaries collected on days 50 and 140 of gestation and on days 2 , 13 , 22 , and 35 after parturition were enzymatically dissociated and the resulting cells cultured in media containing estradiol ( 12 pg/ml ) , cortisol ( 12 ng/ml ) , or no steroid .
C0032961
gestation
C0014912
estradiol