the following mutants of human tissue-type plasminogen activator ( t-pa ) were constructed by deletion mutagenesis of t-pa cdna , expressed in chinese hamster ovary cells and purified to homogeneity : ( a ) t-pa-delta fe : t-pa lacking both the fibronectin fingerlike ( f ) domain and the epidermal growth factor ( e ) domain , ( b ) t-pa-delta fe1x : t-pa-delta fe with the glycosylated 117asn mutagenized to gln , and ( c ) t-pa-delta fe3x : t-pa-delta fe with the three known glycosylated asn residues replaced by gln .
C0086418
human
C0242275
epidermal growth factor