the low affinity showed a marked dependence upon haemoglobin concentration which was absent for 2,3-dpg-depleted hb-a cells and , by extrapolation , the mchc at which the oxygen affinities of the hb-s cells became identical to that of the hb-a cells was 14.5 g/dl in phosphate buffer and 13.1 g/dl in bis tris .
C0019016
a
C0019046
hb