after pulverization of tissue , one part was analyzed by the routine dextran-coated charcoal ( dcc ) method and the other by a micromethod as follows : ( i ) cytosol incubation using the dcc method but in the simultaneous presence of [ 3h ] oestradiol and [ 3h ] r5020 ( ii ) extraction of the steroids bound to the receptor by precipitation with ethanol/tca ( iii ) high pressure liquid chromatography ( hplc ) on a modular system , with a c185 microns column and an elution by gradient mixture methanol/water .
C0001962
ethanol
C0001963
methanol