both processes were energy dependent , inhibited by cold ( 4 degrees c ) , 2-deoxyglucose , and azide , and both required no de novo protein synthesis as they were not affected by pretreatment of the cells with the irreversible protein inhibitor pactamycin . trypsin treatment of antigen-pulsed ebv-b cells and monocytes followed by fixation for 1 min in 0.03 % paraformaldehyde completely abolished the capacity of both cell types to induce t cell proliferation .
C0007634
cells
C1155046
t cell proliferation