the selective carboxymethylation by iodoacetate of cys-46 in the active center of horse liver alcohol dehydrogenase has been shown to be mediated by interaction of the anionic reagent with the arginyl residue ( s ) previously shown to be responsible for binding nadh ( l.g . lange , j.f . riordan , and b.l . vallee ( 1974 ) , biochemistry 13 , 4361 ) .
C0003075
anionic
C0016327
f