in the present study we developed an immunohistochemical method for visualizing tubulin and actin in rat hepatocytes in situ , using a perfusion extraction-fixation procedure , in which the liver was perfused through the portal vein with a nonionic detergent to make the plasma membranes permeable to soluble substances , followed by a fixative to preserve cytoskeletal structure .
C0001271
actin
C0010853
cytoskeletal