in an analysis of the limitations on transcription under these conditions , it was found that transcription could be increased drastically not only by ie addition but also by ( 1 ) the addition of nonpromoter-containing dna , which titrated nonspecific dna-binding proteins in the crude nuclear extract , and ( 2 ) preincubation of the template with either the nuclear extract ( in the absence of mg2+ ) or with the tata box-binding factor , tfiid .
C0144555
tata
C0076919
tfiid