we have used mono s cation-exchange chromatography to prepare milligram quantities of pure ser 6-phosphorylated hmg 14 ( ser 6-po4-hmg ) from unphosphorylated mono s-purified calf thymus hmg 14 following in vitro phosphorylation with camp-dependent protein kinase ( a-kinase ) .
C0031715
phosphorylation
C0031727
kinase