the pbr322 plasmid copy number for media free of amino acids and supplemented with 0.2-0.4 mm fecl3 was 60-80 plasmid copies per cell : ( v ) the combination of fe3+ with certain amino acids ( ala , arg , glu , leu , thr , and trp ) leads to a dramatic increase in the plasmid copy number reaching 180-270 plasmid copies per cell for the plasmid pbr322 and 20-24 for the plasmid pr100 .
C0002520
amino acids
C0061472
glu