in previous work we have shown that puromycin photoaffinity labels two proteins , l23 and s14 , from separate sites of high affinity on escherichia coli ribosomes [ jaynes , e. n. , jr. , grant , p. g. , giangrande , g. , wieder , r. , & cooperman , b. s. ( 1978 ) biochemistry 17 , 561-569 ; weitzmann , c. j. , & cooperman , b. s. ( 1985 ) biochemistry 24 , 2268-2274 ] , that puromycin-modified s14 is separable from native s14 by reverse-phase high-performance liquid chromatography ( rp-hplc ) , and that ribosomal proteins prepared by rp-hplc can be reconstituted into active 30s subunits [ kerlavage , a. r. , weitzmann , c. j. , & cooperman , b. s. ( 1984 ) j. chromatogr . 317 , 201-212 ] .
C0033684
proteins
C0035552
ribosomal proteins