lysis of nocodazole-poisoned cells in a microtubule reassembly buffer containing 0.5 m pipes , 2.5 % dimethyl sulfoxide , 1 mm edta , 1 mm mgcl2 , 1 mm gtp , 1 % triton x-165 , 0.5 % sodium deoxycholate , 0.2 % sds , ph 6.9 , preserved metaphase aster structures 5 micrograms in diameter surrounded only by a thin , fibrous cell remnant .
C0026046
microtubule
C0007634
cell