this phenomenon was found for the first time using special cell-free translation systems ( cfts ) of very different variants of their composition consisting of the following main components : post-mitochondrial supernatant ( pms ) , total cytoplasmic poly ( a ) + mrna or a species-specific poly ( a ) + mrna isolated form the pms by affinity chromatography on the columns with the anti-mrna1-fab- ( cnbr ) -sepharose , or purified 9s or 11s globin or histone specific mrnas , respectively , and , finally a few samples of the cfts used contain the additions of high or low molecular weight cytosolic compounds isolated from s150 fraction by ultrafiltration on diaflo um2 membrane with an exclusion limit of 1.0 kd .
C0010834
cytoplasmic
C1383501
cytosolic