as measured with conventional single-barrelled microelectrodes , acetylcholine ( ach ) , nicotine , carbachol , tetramethylammonium ( tma ) , and choline elicited concentration-dependent ( 10 ( -6 ) -5 x 10 ( -3 ) m ) transient membrane depolarizations of up to 60 mv amplitude whereas muscarine ( 10 ( -6 ) -10 ( -3 ) m ) did not affect em . alpha-bungarotoxin ( 10 ( -7 ) m ) , decamethonium ( 10 ( -5 ) m ) , d-tubocurarine ( 5 x 10 ( -5 ) m ) , and strychnine ( 5 x 10 ( -5 ) m ) blocked the carbachol depolarization by about 90 % .
C0008405
choline
C0006945
carbachol