a series of extraction procedures were applied to avian nuclei which allowed us to define three types of association of v-myc- and c-myc-encoded proteins with nuclei : ( i ) a major fraction ( 60 to 90 % ) which is retained in dna- and rna-depleted nuclei after low- and high-salt extraction , ( ii ) a small fraction ( 1 % ) released during nuclease digestion of dna in intact nuclei in the presence of low-salt buffer , and ( iii ) a fraction of myc protein ( less than 10 % ) extractable with salt or detergents and found to have affinity for both single- and double-stranded dna .
C0080065
c-myc
C0007610
nuclei