site-directed deletions as well as point mutations were introduced into the genes encoding human tissue plasminogen activator ( tpa ) and the bacillus amyloliquefaciens alpha-amylase gene using lambda exonuclease to enzymatically degrade dna 5 ' to 3 ' in order to generate a single-stranded template in the immediate vicinity of the oligonucleotide annealing site .
C0086418
human
C1537647
alpha