in this report , we demonstrate that the ra-5-1 mutant possesses a prolyl-4-hydroxylase enzyme with a higher km for a synthetic collagen substrate and that this alteration results in a 6-7-fold reduction in the amount of collagen iv in the medium of ract-treated mutant cells , as compared to wild type f9 cells .
C0009325
collagen
C0009333
collagen iv