in this limiting dilution ( ld ) culture system , responder cells ( unseparated mononuclear cells , e rosette-purified t cells , or cell sorter-separated cd4+ and cd8+ subsets ) were activated by irradiated anti-cd3-secreting ( okt3 ) hybridoma cells in the presence of exogenous il-2 ( crude culture supernatant or recombinant il-2 ) .
C0007634
cells
C1327616
secreting