a procedure involving digestion of minced glands with a solution of 0.1 % trypsin in s-mem tissue culture medium ( 0.1 % bsa ) for 1 hr at 37 degrees under an atmosphere of 5 % co2-95 % air yielded greater than 2.0 x 10 ( 6 ) cells per gland with 80-90 % viability .
C0012238
digestion
C1418965
1