the true substrate for the pyruvate kinase catalyzed phosphorylation of hydroxylamine at high ph which is activated by bicarbonate is shown to be n-hydroxycarbamate , since a lag is seen when the reaction is started by the addition of bicarbonate or hydroxylamine but a burst appears when it is started with a mixture of the two .
C0034348
pyruvate kinase
C0031715
phosphorylation