depletion of the histone h1from nuclei results in the transition from low to high affinity of a portion of binding sites , as shown by 1 ) the increase in fluorescence intensity after staining with the dye at low saturating concentrations and 2 ) the higher value of the fluorescence intensity ratio ( fi5/fi50 ) exhibited by h1-depleted nuclei stained with a low ( 5 micrograms/ml ) vs. a high ( 50 micrograms/ml ) concentration , as compared with control samples .
C0019652
histone
C0007610
nuclei