after maximum cell growth , the cells were inoculated with chikungunya virus at a multiplicity of 1 to 2 50 % suckling mouse intracerebral lethal doses ( smicld ( 50 ) ) per cell in the spent eagle 's minimum essential medium for suspension cultures ( mems ) , or the cell cultures were centrifuged at 200 x g and resuspended in either fresh mems or medium 199 prior to inoculation .
C0007595
cell
C0007634
cell