pure 2-amino-3-ketobutyrate coa ligase from escherichia coli , which catalyzes the cleavage/condensation reaction between 2-amino-3-ketobutyrate ( the presumed product of the l-threonine dehydrogenase-catalyzed reaction ) and glycine + acetyl-coa , is a dimeric enzyme ( mr = 84,000 ) that requires pyridoxal 5'-phosphate as coenzyme for catalytic activity .
C0034266
pyridoxal
C0009235
coenzyme