by filling the wells in the agar plate with a 1:4 diluted heat-inactivated sera and covering the agar surface with a complement film , a zone of unlysed cells surrounded by a hemolytic area appears after incubation overnight at 4 degrees c and then for 1-2 h at 37 degrees c , depending on the antibody titers .
C0229671
sera
C0009498
complement