by combining several techniques ( physiological studies , microinjections , inhibition of fatty acid synthesis , and freeze-fracture analysis ) we demonstrate ( a ) that the product of the mutated allele nd9 is not thermolabile but that its activity is dependent upon temperature-induced changes in the membrane lipid composition and ( b ) that the product of the nd9 locus is a diffusible cytoplasmic component whose interaction with both plasma membrane and trichocyst membrane is required for rosette assembly and exocytosis .
C0010834
cytoplasmic
C0007603
plasma membrane