to determine whether potential differences in cytoskeletal proteins develop with progressive subcultivation , early and late passage cultures were extracted with 0.5 % triton x-100 for 1 min followed by 1.0 % sodium dodecyl sulfate ( sds ) prior to separation and characterization of extracted proteins by electrophoresis on 7.5-15 % gradient sds gels .
C0010852
cytoskeletal proteins
C0033684
proteins