following incubation of fibroblasts with 2 nm [ 3h ] dht ( 45 min , 37 degrees c ) , dht-r were prepared as total fibroblast sonicates ( sonication of cells in 0.5 m kcl ) , intact fibroblast cytosol ( 100000 x g supernatant ) or intact fibroblast nuclear extract ( sonication of nuclei in 0.5 m kcl ) .
C0007634
cells
C1383501
cytosol