in order to resolve the observation that addition of glutamine and glutamate appears to be of particular importance in enhancing the activity of a cell-free protein synthesis system derived from rat liver ( manchester and tyobeka , 1980 ) , we have measured the km of the aminoacyl-trna synthetases towards amino acids and the extent of aminoacylation of trna under the conditions of our earlier experiments .
C0017797
glutamine
C0002520
amino acids