this is accomplished by floating the sections on pronase ( 1 mg/ml ) for 15 min at 30 degrees , washing the sections three times , and digesting with rnase at high ionic strength for 90 min at 30 degrees . when the washing steps and the rnase hydrolysis are carried out at low ionic strength , dsrna is completely hydrolyzed .
C0014241
rnase
C0020291
hydrolysis