the technique involves four sequential steps : ( i ) adhesion of fixed or fresh homogenates or tissue sections to wells of microtiter plates , ( ii ) binding of a monoclonal antibody against phosphorylated neurofilament to the tissue , ( iii ) secondary binding to the anti-phosphorylated neurofilament of a phosphatase-labeled second antibody ( antimouse igg ) , and ( iv ) enzymatic assay of alkaline phosphatase activity using a fluorescent substrate ( 4-methylumbelliferyl phosphate ) .
C0003250
monoclonal antibody
C0003241
antibody