after mononuclear cells obtained from peripheral blood were cultured in the presence of phytohemagglutinin stimulated-leukocyte conditioned medium ( pha-lcm ) for 7 days ( the first phase ) , nonphagocytic cells were recultured under hypoxic culture conditions containing 30 % fetal calf serum , 1 % bovine serum albumin , 300 micrograms/ml transferrin and 2 u/ml recombinant erythropoietin ( the second phase ) .
C0229664
peripheral
C0023516
leukocyte