monoclonal t cell colonies can be grown in agar culture from quiescent t lymphocytes under pha stimulation , provided that ( 1 ) a low number of t lymphocytes ( less than or equal to 5 x 10 ( 4 ) /ml ) is seeded , ( 2 ) il-2 is added to the culture , and ( 3 ) a high number of accessory b cells ( greater than or equal to 5 x 10 ( 5 ) /ml ) is present in contact with the t lymphocytes .
C1155065
t
C0039194
t lymphocytes