these tested experimental predictions include ( 1 ) instability of the drug-dna adducts to denaturation of dna , ( 2 ) saturation binding limits , ( 3 ) effect of drug binding on the structure of dna , ( 4 ) lack of unwinding and in vitro strand breakage of closed-circular supercoiled simian virus 40 ( sv-40 ) dna , ( 5 ) sensitivity of the secondary structure of dna to drug binding , ( 6 ) hydrodynamic properties of the drug-dna adducts , ( 7 ) hydrogen bonding of the 9-phenolic proton in anthramycin to dna , ( 8 ) structure-activity relationships , and ( 9 ) biological consequences of dna damage , including cumulative damage and slow excision repair , double-strain breaks in dna in repair-proficient cells , and the selective inhibition of h-strand dna synthesis in mitochondria .
C0007634
cells
C0026237
mitochondria