at 30 degrees c , ph 8.0 ( 0.1 m tris-chloride buffer ) , 5.5 microm aps , 5 mm mgatp , 5 mm excess mgcl2 , and `` '' high '' '' salt ( 70-150 mm ( nh4 ) 2so4 ) , the most highly purified preparation has a specific activity of 24.7 units x mg of protein-1 in the physiological direction of adenosine 3'-phosphate 5'-phosphosulfate ( paps ) formation .
C0008203
chloride
C0024472
mgcl2