the cdna encoding a low mr derivative ( residues 144-411 ) of human single-chain urokinase-type plasminogen activator was cloned , the recombinant low mr single-chain urokinase-type plasminogen activator ( rscu-pa-32k ) was expressed in chinese hamster ovary cells , and the translation product was purified to homogeneity from conditioned cell culture medium . rscu-pa-32k is very similar to intact recombinant single-chain urokinase-type plasminogen activator in terms of its very low activity ( 120 iu/mg ) on a chromogenic substrate for urokinase ( pyroglutamylglycylarginine p-nitroanilide ) , its plasminogen-dependent fibrinolytic activity on fibrin plates ( specific activity = 170,000 iu/mg ) , its plasminogen activating potential , and the lack of specific binding to fibrin .
C0086418
human
C0042071
urokinase