muscarinic acetylcholine receptors purified from porcine cerebra or atria were covalently labeled with [ 3h ] propylbenzilylcholine mustard ( [ 3h ] prbcm ) , and then the labeled receptors were subjected to limited hydrolysis with trypsin , v8 protease , and lysyl endopeptidase , followed by analysis involving sodium dodecyl sulfate-polyacrylamide gel electrophoresis , fluorography , autoradiography , or immunostaining .
C0020291
hydrolysis
C0030946
protease