in agreement with previous investigators who have employed the rodent bone cell system , we confirmed in bovine bone cells that ( a ) there was a typical cyclic amp response to parathyroid hormone , ( b ) freshly isolated cells possessed high levels of alkaline phosphatase , which diminished during culture but returned to normal levels in mineralizing cultures , and ( c ) cells grown in the presence of ascorbic acid and beta-glycerophosphate rapidly produced and mineralized an extracellular matrix containing largely type i collagen .
C0015350
extracellular matrix
C0041455
type i