a method for the determination of the catalytic parameters ks , k+2 and k+3 describing trypsin-like serine proteinase action has been developed from the quantitative analysis of the kinetics of hydrolysis of two specific chromogenic substrates , n-alpha-carbobenzoxy-l-arginine p-nitrophenyl ester and n-alpha-carbobenzoxy-l-lysine p-nitrophenyl ester , catalyzed by porcine pancreatic betta-kallikrein b , bovine betta-trypsin and human urokinase ( mr 54,000 species ) , under conditions where the concentration of the substrate exceeds that of the enzyme .
C0086418
human
C0042071
urokinase