the resultant filling reveals detailed neuronal morphology of somata , dendrites , and their appendages offering the following advantages over currently available cell staining techniques : ( 1 ) a large number ( 10-30 ) of neurons can be filled intracellularly in a single experiment , ( 2 ) when coupled with retrograde fluorescent tracing methods , specific populations of neurons can be chosen for study , ( 3 ) good results can be obtained reliably , ( 4 ) neurons from autopsy or biopsy material can be intracellularly filled , ( 5 ) other neuronal features can be identified by combining this method with lectin or neurotransmitter immunocytochemistry , and ( 6 ) filled cells can be made electron dense , permitting specimens to be examined under the electron microscope .
C0011305
dendrites
C0027882
neurons