two methods to obtain lymphocyte subpopulations , defined by specific surface receptors , from rabbit peripheral blood cells were compared as to cell recovery , yield and purity of the obtained fractions : a ) the formation of rosettes between lymphocytes and srbc or srbc coated with an antigen -- antibody -- complement complex ( d-srbc ) , followed by isolation of the rosettes and recovery of the rfc , b ) retention of surface-ig bearing cells on an immunoadsorbent to which antibody to rabbit ig was covalently attached via a digestible gelatin bridge , with subsequent recovery of the retained cells by the enzymatic digestion of the bridge .
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of surface
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cells