we tested 5 different conditions of stringency with biotinylated hpv 1 , 2 , 16 and 18 probes on 3 cell lines ( siha and caski with hpv16 , hela with hpv18 ) by varying the concentration of formamide in the hybridization mixture and washings in order to determine the stringency conditions to be used to assess the presence of hpv and its typing : a-low stringency , hybridization at 35 degrees c below the melting temperature of dna ( tm-35 degrees c ) and washings without formamide ;
C0999806
hpv16
C0021344
hpv