by contrast , when cells were extracted with 0.1 % triton x-100 prior to fixation with paraformaldehyde , the staining patterns obtained with antibodies to aminoacyl-trna synthetases were very similar to that obtained with antibodies to rough endoplasmic reticulum , as assessed by single or double indirect immunofluorescence microscopy .
C0007634
cells
C0230779
rough endoplasmic reticulum