incubation of [ gamma-32p ] atp with a molar excess of the soluble , homogeneous atpase from beef heart mitochondria ( f1 ) results in binding of substrate primarily in a single , very high affinity ( ka = 10 ( 12 ) m-1 ) catalytic site and in a slow rate of hydrolysis characteristic of single site catalysis .
C0001473
atpase
C0020291
hydrolysis