by using a potent inhibitor of the 44/62 atpase , atp gamma s ( adenosine 5'-o- ( 3-thiotriphosphate ) ) , we have obtained data which suggest that atp hydrolysis is required for the formation of a polymerase accessory protein-dna template complex , and that this complex then persists , serving as a sliding clamp which greatly increases the strength of binding between a t4 dna polymerase molecule and its 3'oh primer template end .
C0001473
atpase
C1510699
atp hydrolysis