purification of pcs , including anion-exchange chromatography , ods c-18 reverse-phase hplc , and affinity chromatography , showed that a biologically active peak , eluted in 32 % acetonitrile , contains a 32- to 34-kda protein which is 600-fold more potent than the crude extract is a test involving the hypocalcemic response in the cs-deprived eel .
C0030520
pcs
C0020598
hypocalcemic