evidence is presented that the exogenously supplied c6-nbd-pi was hydrolyzed by a calcium-dependent pi-specific phospholipase c ( pi-plc ) at the external cell surface and that this pi-specific phospholipase c may play a role in the density-dependent inhibition of cell growth : ( i ) when confluent monolayer cultures were incubated with c6-nbd-pi for 60 min at 7 degrees c , the lipid spontaneously transferred to the cells , and prominent labeling of intracellular membranes was observed .
C0007634
cells
C0021868
intracellular membranes