the dna-dependent rna polymerase i ( or a ) from the lower eukaryote aspergillus nidulans has been purified on a large scale to apparent homogeneity by homogenizing the fungal hyphae in liquid nitrogen , extraction of the enzyme at high salt concentration , precipitation of rna polymerase activity with polymin p ( a polyethylene imine ) , elution of the rna polymerase from the polymin p precipitate , ammonium sulphate precipitation , molecular sieving on bio-gel a-1.5m , binding to ion-exchangers and dna-cellulose affinity chromatography .
C0035678
dna-dependent rna polymerase i
C0035681
rna polymerase