if soft agar colony formation assays for primary human tumor cells are going to be performed and the results assessed by optical analysis of colony formation , then we believe it is mandatory in such assay techniques to objectively count control plates on the day of culture initiation using stained plates , use universal cytotoxic control compounds which should uniformly eliminate all viable cell proliferation , and use a vital stain such as the tetrazolium dye int to document the presence or absence of viable cell colonies when cultures are assessed .
C0596290
cell proliferation
C0007634
cell