the free energy of phosphoenolpyruvate binding to free enzyme ( ks = 1.58 +/- 0.10 x 10 ( -4 ) m ; deltaf degrees = -5.13 +/- 0.04 kcal/mol ) and to the enzyme-mn2+ complex ( k3 = 0.75 +/- 0.10 x 10 ( -6 ) m ; deltaf degrees = -8.26 +/- 0.07 kcal/mol ) also gives a large negative free energy of coupling , delta ( deltaf degrees ) = -3.16 +/- 0.08 kcal/mol .
C0014442
enzyme
C0026741
enzyme