the identity of the cdna clones was verified by multiple criteria : 1 ) beta-galactosidase fusion proteins synthesized by isolated cdna clones stain intensely with camp receptor directed antiserum , 2 ) these fusion proteins affinity purify antibodies specific for the camp receptor , 3 ) the cdna probes hybridize to a 2 kb mrna whose change in relative level of abundance during development parallels that of receptor mrna as assayed by in vitro translation , 4 ) the 2 kb mrna size equals that of receptor mrna as determined by in vitro translation of size fractionated poly ( a ) + rna , and 5 ) rna transcribed in vitro from cdnas containing the entire protein-coding region produces a polypeptide by in vitro translation with an apparent molecular weight in close agreement with that of nascent camp receptor protein produced by in vitro translation of cellular rna .
C0079941
protein-coding region
C0007634
cellular