insoluble bone gelatin produces new bone after extraction at 2 degrees with neutral salts , 0.5 m edta , 0.1 m tris.hcl , 4 m urea , 0.5 m hydroxylamine , and 10 m kcns , as well as after limited digestion with pepsin or collagenase , but not after extraction with 5 m guanidine , 7 m urea , water saturated with phenol , or after alkali hydrolysis with 0.1 n naoh .
C0162745
collagenase
C0020291
hydrolysis