the method involves ( 1 ) saturating a small column of phenyl-sepharose ( 1-2 ml ) with one of the alkyl detergents at a ph of 8 or 9 and an ionic strength of 0.01 , ( 2 ) applying a detergent-solubilized membrane protein sample containing as much as 20 mg/ml of triton x-100 , triton n-101 , or nonidet p-40 , and ( 3 ) eluting the protein with buffer containing the detergent with which the resin had been saturated .
C0025252
membrane protein
C0033684
protein