the procedure consists of acetylation of the total lipids after extraction , separation of acetylated glycophingolipids from non-glycolipids on a florisil column , deacetylation , dialysis in water , separation of the single fractions by thin-layer chromatography on silica gel , and quantitative analysis of each glycosphingolipid by sulfuric acid-orcinol reagent , carried out in the presence of silica gel .
C0017950
glycolipids
C0017978
glycosphingolipid