by this method we have purified the following t7 replication-related proteins ( each greater than 50 % pure as judged by sodium dodecyl sulfate gel electrophoresis ) : t7 dna-binding protein ( 27,000 daltons ) , t7 rna polymerase ( 105,000 daltons ) , t7 dna polymerase ( gene 5-protein , 85,000 daltons , plus host-factor ) , t7 dna ligase ( 40,000 daltons ) , and t7 dna-priming protein ( 65,000 daltons ) .
C0033684
proteins
C0012940
dna-binding protein