a rapid procedure for the isolation of functional enhancer sequences consists of the construction of a shotgun dna library in sv40-based plasmid shuttle vectors which depend on an enhancer for replication , the replication in monkey ( cvi ) cells of those vectors into which an enhancer sequence was inserted , the selective cleavage of unreplicated vectors by dpni and the recovery of the replicated vectors by transfection into escherichia coli .
C0012854
dna
C0598312
replication