when living cells of nitella are first exposed to ( 1 ) phosphate buffer mixture , or ( 2 ) phosphoric acid , or ( 3 ) hydrochloric acid , or ( 4 ) sodium chloride , or ( 5 ) sodium borate , and are then placed in a solution of brilliant cresyl blue made up with a borate buffer mixture at ph 7.85 , the rate of penetration of the dye into the vacuole is decreased as compared with the rate in the case of cells transferred directly from tap water to the same dye solution .
C0042219
vacuole
C0007634
cells