a comparison of the mutant and control strains revealed that ( i ) the mutant lacked detectable penicillin-binding protein 5 , ( ii ) the d-alanine carboxypeptidase activity of membranes isolated from the mutant was only 5 % of that measured in membranes from the control strain , ( iii ) the mutant cells showed apparently normal morphology only during exponential growth , and after the end of exponential phase the cells became progressively shorter , ( iv ) the mutant sporulated normally except that the forespore occupied about two-thirds of the mother cell cytoplasm and , during its development , migrated towards the center of the mother cell , and ( v ) purified mutant spores were 10-fold less heat resistant but possessed normal refractility and morphology .
C0007634
cells
C0010834
cytoplasm