leptospira interrogans serotype canicola ( strain nadl a-13 ) grew from inocula as small as two cells in liquid polysorbate 80 medium ( p-80 medium , in p-60 , p-40 and p-20 media , and in p-80 medium from which polysorbate , nh4c1 or thiamine had been omitted .
C0032601
polysorbate 80
C0032602
polysorbate