when the metal ion complexing reagents ethylenediaminetetraacetic acid , phosphate ion , hydroxyl ion , or the competitive cations zn2+ or ca2+ are added to the sodium dodecyl sulfate-solubilized sample of outer membrane , and then the sample is heated to 100 degrees c and recooled to room temperature , the protein is almost completely converted to the high-temperature form .
C0022023
ion
C0007447
cations