in the first step , acac and pyruvate are completely reduced , using 3-hydroxybutyrate dehydrogenase ( ec 1.1.1.30 ) and lactate dehydrogenase ( ec 1.1.1.27 ) in the presence of excess nadh at ph 7.5 , to beta-hydroxybutyrate and lactate , respectively .
C0030016
ec 1
C0022917
lactate dehydrogenase