based on the premise that cell-surface proteins involved in focal or close cell-to-substrate contact will be linked across the membrane , either directly or indirectly to f-actin of the cytoskeleton , we have used a modification of the myosin affinity technique ( leonardi , c l & rubin , r w , anal biochem 118 ( 1981 ) 58 ) to precipitate f-actin filaments and associated proteins from nonidet p-40 solubilized cells .
C0007634
cell
C0010853
cytoskeleton