the fluorescent indicator fura-2 has been applied to a variety of cell types in order to set up appropriate conditions for measurements of the cytosolic concentration of free ionized ca2+ [ ( ca2+ ] i ) in both cell suspensions and single cells analyzed in a conventional fluorimeter or in a fluorescence microscope equipped for quantitative analyses ( with or without computerized image analyses ) , respectively .
C1383501
cytosolic
C0007634
cells