to locate the molecular domains involved in the formation of these heterotypic complexes , we have developed a binding assay in which total cellular or cytoskeletal polypeptides , or proteolytically prepared cytokeratin fragments , are separated by one- , or two-dimensional gel electrophoresis , blot-transferred on to nitrocellulose paper and probed with radio-iodinated purified cytokeratin polypeptides or fragments thereof , using buffers of various ionic strengths with or without 4 m-urea .
C0010853
cytoskeletal
C0010803
cytokeratin