initial studies using a model system of hsv-1 or hsv-2-infected vero cells established ( i ) acetone as the most effective fixative , ( ii ) optimal dilutions of preimmunization and anti-hsv serum for differentiation of infected from noninfected cells , ( iii ) optimal concentration of 3,3'-diaminobenzidine tetrahydrochloride and h2o2 for maximal staining of infected cells with minimal background reaction , and ( iv ) removal of endogenous peroxidase with absolute meoh .
C0007589
differentiation
C0007634
cells