the recombinant toxin , which was located in the cytoplasm of e. coli , was purified by affinity chromatography with immobilized monoclonal antibody and was shown to react in a manner identical to that of pmt in a quantitative structural test using a series of monoclonal antibodies as well as in all quantitative functional tests used , i.e. , tests for dermonecrotic activity and mouse lethality and the embryonic bovine lung cell test for cytopathic activity .
C0010834
cytoplasm
C0007634
the