the method involves two successive enzymatic reactions : conversion of donor [ gamma-32p ] atp in the presence of an excess of acceptor 5'-mononucleotide to the 5'-diphosphates by myokinase or guanosine 5'-monophosphate kinase followed by phosphorylation with pyruvate kinase to yield 5'-triphosphates .
C0031715
phosphorylation
C0034348
pyruvate kinase