the method involves ( a ) brief fixation with formaldehyde-containing fixatives either prior to freezing or immediately after cutting cryostat sections , ( b ) bleaching the tissue of endogenous peroxidase activity , ( c ) application of primary antibody or control immunoglobulin , ( d ) application of an adsorbed bridging antibody ( goat antirat igg ) , and ( e ) application of rat peroxidase-antiperoxidase followed by diaminobenzidine .
C0021027
immunoglobulin
C0003241
antibody