washed cells were broken in a french press and , after deoxyribonuclease and ribonuclease treatment , removal of intact cells , and four washes in 20 mosmol phosphate buffer , ph 7.4 , with centrifugation at 25,000 x g for 10 min , preparations of cell wall fragments from which almost all pieces of plasma membrane had been removed resulted .
C0007634
cells
C0007603
plasma membrane