the expressed protein was purified to homogeneity by anion exchange and blue sepharose chromatography and had kinetic and physical properties similar to the purified rat liver enzyme , including its behavior as a dimer during gel filtration , activation of the kinase by phosphate and inhibition by alpha-glycerol phosphate , and mediation of the bisphosphatase reaction by a phosphoenzyme intermediate .
C0003075
anion
C0031603
phosphate