to determine the effect of fluoride on the expression of these cells , primary rc cells were grown for periods of up to 21 days in alpha-mem ( minimal essential medium ) containing 5-15 % heat-inactivated fetal bovine serum ( fbs ) , 50 micrograms/ml ascorbic acid , 10 mm na beta-glycerophosphate , and naf at concentrations from 10 microm to 5 mm .
C0016327
fluoride
C0037508
naf