the cellular interphase from three fractions ( f x ) corresponding to specific densities 1.050 ( f x 1 ) , 1.055 ( f x 2 ) , and 1.060 ( f x 3 ) as well as cells separated by ficoll-diatrizoate ( f x fd ) centrifugation were isolated and 2.5 x 10 ( 5 ) cells from each fraction were cultured in 2 ml of 0.3 % agar in mccoy 's media with 10 % fetal calf serum , 2.5 micrograms transferrin , 1 microgram insulin , and 1 % penicillin-streptomycin .
C0021798
interphase
C0007634
cells