the unmounted sections are examined and photographed in the fluorescence microscope , and then stained for acetylcholinesterse according to holmstedt 's modification of the koelle thiocholine method ( incubation for 4-6 h with acetylthiocholine as substrate and mipafox as inhibitor of non-specific cholinesterases ) .
C0039883
thiocholine
C0001066
acetylthiocholine