( 2 ) slow cooling with seeding at -8 degrees c gives an extracellular crystallization which is achieved by -9 degrees c , followed by an extracellular recrystallization occurring at almost -8 degrees c which alters the morphology of the oocyte and the zona pellucida , without any visible intracellular crystallization ;
C0029045
oocyte
C0043519
zona pellucida