the following procedures were associated with high rates of mouse embryo development and human pregnancies following ivf/et : adequate gassing and equilibration of the medium , double-rinsing of pipets and catheters used to handle embryos , use of a hepes-buffered medium for manipulating embryos in the absence of an atmosphere containing 5 % co2 , control of excessive temperature in the vicinity of the embryos , and et using medium containing 50 % patient 's serum .
C0086418
human
C1419864
ivf