focusing our attention on the effect of the anions on the functional properties of hemoglobin , we measured the bohr effect of untreated hemoglobin in buffers made with hepes [ n- ( 2-hydroxyethyl ) piperazine-n'-2-ethanesulfonic acid ] , mes [ 2- ( n-morpholino ) ethanesulfonic acid ] , and mops [ 3- ( n-morpholino ) propanesulfonic acid ] , which being zwitterions do not need addition of chlorides or other anions for reaching the desired ph .
C0008203
chlorides
C0003075
anions