cultures of pbmc ( 5 x 10 ( 6 ) /ml ) were incubated for 24 hr in media only ( control , 1,000 u/ml ril-2 , 2 micrograms/ml lps , or both lps and ril-2 . supernatants obtained from these cultures were analyzed for levels of il-1 beta and pge2 by radioimmunoassays . the addition of ril-2 caused an increase in il-1 beta production in 13 of 13 control pbmc cultures and in 11 of 13 lps-stimulated cultures , which were significant increases as determined by paired t tests . when pbmc were fractionated into plastic adherent and nonadherent populations , the ril-2 induced increases in il-1 beta production were more consistent in control ( six of seven cases ) and lps ( seven of seven cases ) cultures of plastic nonadherent cells than in control ( three of seven cases ) and lps ( four of seven cases ) cultures of plastic adherent cells . recombinant il-2 did not increase pge2 production in control pbmc cultures ( none of four cases ) , but did so in lps-stimulated pbmc cultures ( three of four cases ] .
C0007634
cells
C1321301
pbmc