mitochondrial subfractions were isolated after disruption of this subcellular organelle by ( a ) hypotonic lysis in water , which permitted the ultracentrifugal separation of the soluble and membranous compartments of the mitochondrion , or by ( b ) a procedure involving swelling , contraction , and ultrasonic treatment , which permitted the isolation from discontinuous sucrose gradients of subfractions rich in intermembrane space protein , outer membrane , and inner membrane-matrix particles .
C0026237
mitochondrial
C1179889
intermembrane space