the first step is catalyzed by the nad-dependent alcohol dehydrogenase in c. kluyveri , the second by the recently detected enoate reductase which reduces not only nonactivated alpha , beta-unsaturated acylates but also alpha , beta-unsaturated aldehydes in a nadh-dependent reaction and the third step is again catalyzed by alcohol dehydrogenase .
C0030016
reductase
C0001942
alcohol dehydrogenase