thus if an excess of bilirubin is added to human serum albumin or to neonatal serum in buffer containing 0.1 mol of tris ( hydroxymethyl ) aminomethane and 2.0 mol of nacl per liter ( ph 7.5 ) , and the bilirubin not bound to albumin is removed by treatment with calcium carbonate , the bilirubin binding reserve of primary binding sites can be estimated from direct measurement of spectral absorbance at 468 nm .
C0304925
human serum albumin
C0001924
albumin