observations made using indirect immunofluorescence with a monoclonal anti-yeast-tubulin antibody on the morphogenetic mutant cdc4 ( which forms multiple , abnormally elongated buds while the nuclear cycle is arrested ) revealed the surprising occurrence of multiple bundles of cytoplasmic microtubules emanating from the one duplicated spindle-pole body per cell .
C0041348
tubulin
C0026046
cytoplasmic microtubules