d-amino acid transaminase from bacillus sphaericus shows a deuterium kinetic isotope effect ( vh/vd ) between 2 and 3 in the transamination of alpha-protio- or alpha-deuterio-d-alanine and alpha-ketoglutarate , suggesting that alpha-proton abstraction is at least partially rate-limiting for this reaction .
C0002520
amino acid
C0001898
alanine