the method is suggested to isolate simultaneously microsomes and plasma membranes of neuroblastoma s 1300 n 18 cells by means of differential centrifugation in the step density gradient of percoll/ficoll with a high degree of purification determined from the activity of marker enzymes ( acetyl cholinesterase na+ , k+-atpase , alkali phosphatase , glucose-6-phosphatase , succinate-dehydrogenase , acid phosphatase ) as well as from the content of dna and rna and with a sufficiently high protein yield .
C0031678
phosphatase
C0017755
glucose-6-phosphatase