in a serum-free medium [ ham 's f-12 : eagle 's minimum essential medium ( 1:1 , vol/vol ) containing 0.1 % bovine serum albumin ] , the proliferation of sc-3 cells ( a cloned cell line from sc115 cells ) estimated by [ 3h ] thymidine incorporation into dna and cell number reached a plateau at 10 ( -8 ) m testosterone ( up to 200-fold ) , 10 ( -7 ) m dexamethasone ( up to 30-fold ) or 1 ng/ml of fibroblast growth factors ( fgf ; up to 50-fold ) .
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C0016026
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