because the visualization of mts by immunofluorescence is technically difficult in intact hyphae of a. nidulans , we developed a method for removing the cell wall under conditions that do not perturb cell physiology , as evidenced by the fact that the resulting protoplasts undergo nuclear division at a normal rate and that cell cycle mutant phenotypes are expressed at restrictive temperature .
C0007634
the cell
C0007613
cell physiology