in preparation for studies on the growth factor requirements of normal and transformed human fibroblasts , we have developed a serum-free medium that supports vigorous long-term serial subculture of diploid human fibroblasts and allows them to form large-sized colonies with high efficiency ( 40 to 60 % ) when plated at cloning density ( 2 to 5 cells/cm2 ) .
C0016030
fibroblasts
C0007634
cells