the specificity repertoire of splenic lyt-2+/f23+ cytotoxic lymphocyte precursors ( clp ) from b6 mice was investigated in a limiting dilution ( ld ) system designed to support clonal expansion in vitro of a representative fraction of this t-cell subset : in highly purified lyt-2+ responder cells cocultured with mitomycin-treated f23 hybridoma cells in the presence of ( recombinant ) interleukin 2 under ld conditions , one out of three lyt-2+/f23+ clp gave rise to a functional cytotoxic t lymphocyte ( ctl ) clone .
C1516670
clonal expansion
C0007634
cells