acetylcholinesterase ( ache , ec 3.1.1.7 ) from electrophorus electricus , purified by affinity chromatography to a specific activity of 7000-10,000 u/mg protein , was studied at 27 degrees c in conduction-type microcalorimeters for the heats of reaction , with the subsite-specific cationic ligands edrophonium and propidium and with the irreversible inhibitor diisopropylfluorophosphate ( dfp ) , in an ion-free aqueous medium .
C0007447
cationic
C0022023
ion