we studied the poly ( adp-ribosylation ) of the major classes of histones in response to dna breakage induced by an extracellular burst of active oxygen ( ao ) or the alkylating agent n-methyl-n'-nitrosoguandine ( mnng ) in the immortalized human keratinocytes haca t using a combination of affinity chromatography on phenylboronate resin and immunoblotting with polyclonal antibodies against histones h1 , h2b , h2a , h3 , and h4 .
C0086418
human
C0086411
h2b