an increase in the free cytoplasmic ca2+ concentration in thymocytes can be detected by the fluorescent indicator quin 2 within a few seconds of the addition of concanavalin a and the response is quantified from the increased proportion of quin 2 in the cells chelated by ca2+ ( ' % ca-quin 2 ' ) .
C0010834
cytoplasmic
C0007634
cells