the four gyra mutations were located strikingly close to one another within a small region near the n-terminus of the gyra polypeptide , i.e. , nucleotide changes from c to t , from c to g , from g to t and from g to t at nucleotides 248 , 248 , 318 and 199 , respectively , resulting in amino acid changes from ser to leu , from ser to trp , from gln to his and from ala to ser at amino acids 83 , 83 , 106 and 67 , respectively .
C0017797
gln
C0002520
amino