the polyamines , obtained after clean-up of deproteinized serum by cellex p column chromatography , are converted to their fluorescamine derivatives in the presence of nickel ion which inhibits the reaction of interfering amines with fluorescamine , and the derivatives are separated simultaneously by reversed-phase chromatography ( lichrosorb rp-18 ) with a linear gradient elution .
C0032433
polyamines
C0002508
amines