we studied the leukocyte dna of 12 patients with sickle cell anemia , ranging in age from 4 to 61 years and 30 sickle cell trait carriers , ranging in age from 7 to 63 years , for the presence of alpha-globin gene deletions by gene mapping according to southern ( southern em : j mol biol 98:503 , 1975 ) , using alpha- and zeta-globin gene probes obtained by nick translation of the alpha- and zeta-globin genes cloned into plasmid .
C0002895
sickle cell anemia
C0037054
sickle cell trait