in order to elucidate the roles of individual amino acid residues in the conformational stability of proteins , the denaturation by guanidine hydrochloride of the wild-type trytophan synthase alpha-subunit of escherichia coli and two mutant proteins , trpa33 ( glu 49 leads to met ) and trpa11 ( glu 49 leads to gln ) , has been compared by means of cd measurements at ph 7.0 and various temperatures .
C0002520
amino acid
C0061472
glu