human foreskin fibroblasts grew in a serum-free , biochemically defined medium consisting of epidermal growth factor ( 100 ng/ml ) , insulin ( 100 ng/ml ) , transferrin 10 micrograms/ml ) , thrombin ( 1 microgram/ml ) , ascorbic acid ( 10 micrograms/ml ) , and hydrocortisone ( 5 x 10 ( -5 ) m ) in a 1:1 mixture of dulbecco 's modified eagle 's medium and ham 's f-12 , supplemented with ovalbumin ( 1 mg/ml ) and trace elements .
C0086418
human
C0242275
epidermal growth factor