cytoplasmic proteins prepared by repeated freeze-thaw of the hybridoma cells , membrane proteins obtained by np-40 extraction of the cell membrane , and secreted proteins present in the supernates of the various hybridomas were assayed by an enzyme-linked immunoassay ( elisa ) , to understand the discrepancy observed in the immunoglobulins of the cellular and extracellular compartments .
C0007603
cell membrane
C0007634
cellular