after calcium-gel treatment of the crude enzyme extract , ammonium sulfate precipitation , and removal of polynucleotides with deae-cellulose , the enzyme was nonspecifically adsorbed onto phosphocellulose ( p-cellulose ) , and then eluted specifically with a linear concentration gradient of threonine trna from torula yeast ( candida ( torulopsis ) utilis ) .
C0011050
deae-cellulose
C0007648
cellulose