digoxin and its metabolites were separated on a 15 cm x 4.6 mm i.d. , 3-microns reversed-phase ( c18 ) hplc column using methanol -- ethanol -- isopropanol -- water ( 52:3:1:45 ) as the mobile phase at a flow-rate of 0.3 ml/min .
C0001963
methanol
C0001962
ethanol