alkaline phosphatase in c. graniforme is a constitutive enzyme , and examination of the alkaline phosphatase following a purification of 265-fold produced the following characteristics : ph optimum of 9.5 , m ( r ) of 60,000 , k ( m ) of 2.1 x 10 m for p-nitrophenylphosphate , and activation energy for hydrolysis of the substrate at 9.9 kcal ( 1 cal = 4.184 j ) /mol .
C0002059
alkaline phosphatase
C0020291
hydrolysis