we describe a differential extraction system that separates the keratinocyte proteins into four major groups which are further quantitatively analyzed by page : cytoplasmic-soluble proteins , nonionic-detergent-soluble proteins consisting of membrane-associated proteins , salt-dissociated proteins mainly consisting of histones , and ribosomal and keratohyaline granule proteins , and the keratins ( and other intermediate-filament-associated proteins ) , which are further separated into disulfide-stabilized keratins and keratins that do not require reducing agents in order to dissolve in sodium dodecyl sulfate ( sds ) or urea .
C0021769
intermediate-filament
C0010803
keratins