reactions were stopped by dilution and cooling , and rbc bearing antigen-antibody-complement complexes ( ag-ab-c ) were washed and resuspended in either untreated normal human serum ( undiluted or diluted ) , serum treated with zymosan ( szym ) , ethylenediamine tetracetic acid ( sedta ) or ethyleneglycol tetracetic acid-mg++ ( segta ) , serum heated at 56 degrees for 30 or 120 min ( s delta 30 or s delta 120 ) , or buffer alone .
C0009498
complement
C0229671
serum