pursuing the observation of carlier and pantaloni [ carlier , m.-f. , & pantaloni , d. ( 1982 ) biochemistry 21 , 1215-1224 ] that adenosine 5'- ( beta , gamma-imidotriphosphate ) ( pnhppa ) strongly inhibited tubulin-independent phosphatases in microtubule protein preparations , we observed with a number of commercial preparations of pnhppa that a major proportion of the terminal phosphate of [ gamma-32p ] gtp added to microtubule protein preparations was rapidly converted into atp .
C0041348
tubulin
C0026042
microtubule protein preparations