in order to separate [ 14c ] sucrose-sequestering organelles from cytosol , the cells are broken up by electrodisruption -- i.e . a single high-voltage pulse given in a non-ionic medium ( isotonic sucrose ) -- and the resulting cell corpses are sedimented through a dense metrizamide/sucrose cushion to form a cytosol-free pellet containing intact lysosomes .
C0007634
cell
C1383501
cytosol