only readily or commercially available reagents are required : isotype-specific , anti-human ig murine monoclonal antibodies ( mab ) to coat microtiter plates , polyclonal alkaline phosphatase-coupled isotype-specific f ( ab ) ' 2 or fab ' fragments as second antibodies , and an enhanced developing system that amplifies the signal-to-noise ratio of the quantitatively bound second antibody .
C0021017
isotype
C0003241
antibody