to develop a technique for identifying bacillus subtilis genes whose products affect transcription from promoters recognized by sigma 37-containing rna polymerase ( e-sigma 37 ) , we cloned the promoter region of a gene ( ctc ) that is actively transcribed in vitro by e-sigma 37 into a plasmid ( ppl603b ) so that a transcriptional fusion was created between ctc and a plasmid-borne chloramphenicol acetyltransferase ( cat ) gene .
C0035681
rna
C0040649
transcriptional