using the monobromobimane-hplc method it was found that the low-molecular-weight thiol levels in nuclei prepared by detergent permeabilization equilibrate in less than 1 min with the permeabilizing medium , indicating that ( i ) endogenous nuclear glutathione levels can not be determined reliably using conventional methods of cellular disruption and ( ii ) the endogenous nuclear glutathione level is likely to be the same as the cytoplasmic value .
C0007610
nuclei
C0007634
cellular