since a complete map of insulin-related peptides in humans requires consideration of proinsulin , arg32/glu33-split proinsulin , arg65/gly66-split proinsulin , des-arg31 , arg32-proinsulin , des-lys64 , arg65-proinsulin , and insulin , we applied high performance liquid chromatography coupled with radioimmunoassay to investigate the formation of proinsulin conversion intermediates in vitro and in vivo .
C0021641
insulin
C0033362
proinsulin