inositol 1,4,5-trisphosphate ( insp3 ) was introduced into the cytoplasm of characean algae in two different ways : ( i ) by iontophoretic injection into cytoplasm-enriched fragments from chara and ( ii ) by adding insp3 to the permeabilization medium of locally permeabilized cells of nitella .
C0010834
cytoplasm
C0007634
cells