the non-specific cleavage of dna by type ii restriction endonucleases ( bamhi , bsuri , ecori , ecorv , hindiii , psti and sali ) can be effectively suppressed by spermidine in millimolar concentrations , regardless of whether the non-specific cleavage is induced by high concentrations of enzyme under optimal buffer conditions or by high ph , low ionic strength , organic solvents and mn2+ ions .
C0011524
type ii restriction endonucleases
C0011516
ecori