dna ligase was purified about 2,000-fold from blastulae of sea urchin , hemicentrotus pulcherrimus , by means of 1 m kcl-extraction , phosphocellulose , deae-cellulose , sepharose cl-6b , and double-stranded dna cellulose column chromatography .
C0011050
deae-cellulose
C0007648
cellulose