extraction of cells with 0.1 % saponin in microtubule-stabilizing buffer produces holes in the membrane and reorganization of the networks resulting in the loss of microtrabecular structure , the loss of 15 nm filaments and the appearance of abundant membrane-associated microtubules ( about 1.25 micron per micron2 substrate-adherent membrane ) .
C0007634
cells
C0010851
filaments