two findings indicate a concentration-independent net charge of carbonmonoxy hemoglobin at ph 6.6 : ( a ) the ph value of a given carbonmonoty hemoglobin solution remains constant at 6.6 when the hemoglobin concentration is raised from 10 to 40 g/dl , indicating that there is no change in protonation of titratable groups of hemoglobin : ( b ) the net charge of carbonmonoxy hemoglobin as estimated from the donnan distribution of 22na+ shows no dependence on hemoglobin concentration in this concentration range .
C0019016
a
C0019046
hemoglobin