it was previously demonstrated that insulins to which positive charge has been added by substituting b13 glutamic acid with a glutamine residue , b27 threonine with an arginine or lysine residue , and by blocking the c-terminal carboxyl group of the b-chain by amidation , featured a prolonged absorption from the subcutis of rabbits and pigs after injection in solution at acidic ph .
C0061472
glutamic acid
C0017797
glutamine