because synthesis in vitro represents propagation of replication forks active in vivo at the time of permeabilization , our finding that cdc2 and cdc16 mutants can incorporate dtmp into dna in such permeabilized cells at 23 degrees c but not at 37 degrees c supports the conclusion that these two mutations directly affect dna synthesis at replication forks .
C0242961
propagation of
C0007634
cells