apart from human and porcine insulin all the other peptide standards tested ( thyrotropin-releasing factor , vaso-active intestinal polypeptide , human c-peptide , porcine c-peptide , somatostatin , porcine glucagon , porcine proinsulin and porcine pancreatic polypeptide ) could be separated simultaneously in 40 minutes with a binary gradient composed of five linear segments and increasing from 0 to 60 % acetonitrile .
C0006558
c-peptide
C0033362
proinsulin