the enhancement of dna synthesis by epidermal growth factor ( egf ) in cultured human fibroblasts is demonstrable 24 hr after incubation of the cells at 37 degrees c with very low concentrations ( 0.83 nm ) of the hormone for very short periods ( 30 min ) followed by thorough washing of the cells to remove the free hormone in the medium .
C0016030
fibroblasts
C0007634
cells