when a larger excess of ethoxyformic anhydride was used for the lipase reaction , 2.8 histidine residues were ethoxyformylated and characterised as his-354 , his-156 and his-75 , which resulted in an 85 % inhibition of the tributyrylglycerol hydrolysis by the enzyme .
C0023764
lipase
C0020291
hydrolysis