the most striking effect is observed with ternary complexes containing methotrexate or aminopterin and nadph : maximal retention of the labeled protein in the immunoprecipitation assay is reduced to approximately 30 % of its original value with dihydrofolate reductase alone due to a decrease in both the affinity and lifetime of the antibody-protein complex at one or more antigenic sites .
C0025677
methotrexate
C0002583
aminopterin