these in vitro studies demonstrated that : ( 1 ) spleen cells from bcgcw-immunized aci rats had decreased responsiveness to concanavalin a ( con a ) and to alloantigenic stimulation , ( 2 ) depressed con a reactivity could also be induced in buffalo rat spleen cells by the i.p . inoculation of bcgcw , ( 3 ) normal aci rats had suppressor cells in their bone marrow but not in their spleens , ( 4 ) bcgcw-immunized aci rats demonstrated an increase in the suppressive activity of their bone marrow as early as 1 day after inoculation of bcgcw , while suppressor activity was found in the spleen as early as 2 days after bcgcw inoculation , ( 5 ) characterization of the bcgcw-induced splenic suppressor cell demonstrated it to be adherent to plastic or nylon wool , radiation-resistant , and removed by treatment with carbonyl iron .
C0005955
cells
C0005953
bone marrow