specific ab 1-1.1 binding appeared to represent binding to cytoskeletal tubulin inasmuch as : pretreatment of cells with colchicine decreased ab 1-1.1 binding in a dose-dependent manner which correlated with the amount of polymerized tubulin visualized in parallel cultures by indirect immunofluorescence , taxol pretreatment alone caused an increase in ab 1-1.1 binding and prevented in a dose-dependent manner the colchicine-induced decrease in antibody binding , in cells pretreated with colcemid and returned to fresh medium , ab 1-1.1 binding decreased and recovered in parallel with the depolymerization and regrowth of microtubules in these cells , and comparison of maximal antibody binding per cell between primary mouse embryo , 3t3 , and human foreskin fibroblasts correlated with immunofluorescence visualization of microtubules in these cells .
C0010853
cytoskeletal
C0026046
microtubules