the coding segment for mature la was subcloned into the t7 expression system of studier and co-workers ( studier , f.w. , and moffatt , b.a . ( 1986 ) j. mol . biol . 189 , 113-130 ; rosenberg , a.h. , lade , b.n. , chui , d.s. , lin , s.w. , dunn , j.j. , and studier , f.w . ( 1987 ) gene ( amst . ) 56 , 125-135 ) and expressed as a 21-kda fusion protein that consisted of the mature bovine la sequence connected to the nh2-terminal 50 residues of human cathepsin d by a linker sequence containing protease cleavage sites .
C0086418
human
C0007427
cathepsin d