the method is based on ( a ) hydrolysis of cholesterol esters to cholesterol by cholesterol ester hydrolase ( ec 3.1.1.13 ) , ( b ) oxidation of cholesterol to cholest-4-en-4-one and hydrogen peroxide by cholesterol oxidase ( ec 1.1.3.6 ) , and ( c ) oxidation of a triarylimidazole leuco dye with hydrogen peroxide in the presence of peroxidase ( ec 1.11.1.7 ) to produce a dye with maximum absorption at about 650 nm .
C0030011
oxidation
C0027021
peroxidase