to this end , porcine granulosa cells were incubated in monolayer culture for 1-4 days in the presence of fsh ( 200 ng/ml ) , forskolin ( 85 microm ) , or cholera toxin ( 3 micrograms/ml ) with or without an activator of protein kinase c , the phorbol ester 12-o-tetradecanoylphorbol 13-acetate ( tpa ) ( 30 ng/ml ) .
C0031586
phorbol
C0039654
12-o-tetradecanoylphorbol 13-acetate