it was found that digestion with 0.05 % proteinase at 37 degrees c for 20 min and hydrolysis with 1n hcl at 37 degrees c for 20 min was sufficient to detect brdurd immunoreactivity in 3h-thymidine-labelled nuclei , the results being virtually unaffected by the orders in which the two pretreatments were performed .
C0030946
proteinase
C0020291
hydrolysis