removal of the outer cell wall layers of a gram-negative marine pseudomonad ( b-16 ) showed that these cells are penetrable by actinomycin d and that , therefore , neither the cytoplasmic membrane nor the peptidoglycan layer constitutes the barrier which excludes this antibiotic from intact cells , but that this barrier is formed by the outer layers of the cell wall which include the lipopolysaccharide component and the double-track layer .
C0007603
cytoplasmic membrane
C0007634
cell