the method consists of hypotonic shock of intact spermatozoa with 1.25 mm edta to dissociate the plasma membrane and dispersion of these cells to a two-phase polymer system consisting of 5.5 % 252-kd dextran and 4.2 % 20-kd polyethylene glycol prior to centrifugation at 9700 x g for 30 min when the two polymer phases are separated ;
C0007603
plasma membrane
C0007634
cells