in addition to b-50 and its kinase , pkc , the gcp fraction was also shown to contain all other components of such a modulatory system : the phosphatidylinositol 4-phosphate ( pip ) -kinase , as shown on western blots with affinity-purified iggs against pip-kinase , and the polyphosphoinosides , pip and phosphatidylinositol 4,5-bisphosphate ( pip2 ) , since the addition of gamma-32p-atp to the gcp fraction not only results in b-50 phosphorylation but also in the labeling of phosphatidic acid ( pa ) , pip , and pip2 .
C0031727
kinase
C0031715
phosphorylation