normal human melanocytes , which require the phorbol ester 12-o-tetradecanoylphorbol-13-acetate ( tpa ) for growth in culture , were transfected with a sv40 t-antigen-containing plasmid by using the technique of electroporation , and were scored for colonies of morphologically altered cells .
C0031586
phorbol ester
C0039654
12-o-tetradecanoylphorbol-13-acetate