the fluorescence changes that accompany the formation of the three complexes have enabled us to ( 1 ) establish by titration that reca protein binds to 6.0 +/- 0.3 nucleotides of epsilon dna , ( 2 ) show that the binding of atp to the reca-epsilon dna complex is highly cooperative under various conditions , with a hill coefficient of 2.4-4.9 and kapp = 25 +/- 2 micro m , ( 3 ) show that the binding of atp gamma s is also highly cooperative , with a hill coefficient of 3.3-4.2 and kapp congruent to 0.5 micro m , and ( 4 ) perform initial measurements on the rate at which reca protein transfers between polynucleotides .
C0028630
nucleotides
C0032550
polynucleotides