hypothalamic tissue of 4 male rats , bounded by the optic chiasm , mammillary bodies and hypothalamic fissures to a depth of 2-3 mm , was pooled , minced and incubated for 1-3 h in 2 ml of eagle 's minimum essential medium in atmosphere of 95 % o2/5 % co2 with 10 muci of 3h-glycine .
C0024670
mammillary bodies
C0020663
hypothalamic