the trna , with essentially all its uracil and uracil-derived minor bases replaced by fura , was purified by sequential chromatography , first on diethylaminoethylcellulose ( deae-cellulose ) , at ph 8.9 , followed by chromatography on sepharose 4b , using a reverse salt gradient , then on deae-sephadex a-50 , and finally on benzoylated deae-cellulose .
C0041917
uracil
C0016360
fura