specifically , cotransfection of cells with helper virus dna and cloned amplicons ( 8 to 10 kilobases [ kb ] ) containing bacterial plasmid dna sequences linked to a set of herpes simplex virus cis-acting propagation signals ( a replication origin and a cleavage-packaging signal ) resulted in the generation of virus stocks containing packaged defective genomes that consisted of uniform head-to-tail reiterations of the chimeric seed amplicon sequences .
C0007634
cells
C0242961
replication origin