the protein ( s ) responsible for high-affinity nitrobenzylthioinosine binding was purified 13-fold by treatment of haemoglobin-free 'ghosts ' with edta ( ph 11.2 ) to remove extrinsic proteins , extraction of the protein-depleted membranes with triton x-100 and passage of the soluble extract through a deae-cellulose column equilibrated with triton x-100 .
C0033684
proteins
C0025252
protein