inhibitors were purified by paper chromatography and reverse phase high performance liquid chromatography into fractions which block with the same kinetics the incorporation of [ 14 ] leucine and [ 35 ] methionine into protein in systems able to initiate protein synthesis , such as reticulocyte lysates or intact cells , but differ in this respect in incubations of liver ribosomes where re-binding of mrna is a limiting step .
C0597295
protein synthesis
C0035553
ribosomes