vacuoles obtained from cells grown in modified vogel medium ( vegetative-type cells not producing alkaloids ) were isolated and purified by centrifugation from a 5 % ficoll 400 ( wt/vol ) phase into the interphase between two layers , one containing 0.25 m each of mannitol and sucrose , and one containing 0.5 m mannitol .
C0007634
cells
C0021798
interphase