when incubated with 3h-diisopropylfluorophosphate , the enzyme lost all amidolytic activity and yielded a single band of radioactivity in polyacrylamide disk gel electrophoresis in the presence of sodium dodecyl sulfate , and a single peak of radioactivity in gel filtration , both having an apparent molecular weight of 31,000-33,000 ( 31k-protease ) .
C0014442
enzyme
C0030946
protease