however , when membranes were incubated with dithiothreitol ( dtt ) , followed by bam , and subsequently washed , 90 % of the 0.25 nm [ 3h ] [ d-ala2 , ( me ) phe4 , gly ( ol ) 5 ] enkephalin ( dago ) binding was irreversibly inhibited as a result of the specific alkylation of a sulfhydryl group at the mu binding site .
C0014298
enkephalin
C0525773
dago