pluronic f127 polyol , a block copolymer of propylene oxide and ethylene oxide , was studied as an alternative to agar in culture media for nematodes , bacteria , fungi , actinomycetes , and plant tissues or seedlings , at a polyol concentration of 20 % w/v , the culture media , semi-solid at room temperature ( 22 c ) but liquid at lower temperatures , had minimal effects on the test organisms .
C0016832
fungi
C0029235
organisms