proteoliposomes prepared from sonicated asolectin and a highly purified , radiolabeled preparation of the 105,000-dalton hydrolytic moiety of the h+-atpase by a freeze-thaw procedure catalyze atp hydrolysis-dependent proton translocation as indicated by the extensive 9-amino-6-chloro-2-methoxyacridine fluorescence quenching that occurs upon the addition of mgatp to the proteoliposomes , and the reversal of this quenching induced by the h+-atpase inhibitor , vanadate , and the proton conductors , carbonyl cyanide m-chlorophenylhydrazone and nigericin plus k+ .
C0001473
atpase
C1510699
atp hydrolysis