since the site of fixation of cytochrome c was located on the outer side of the inner mitochondrial membrane and since the protein inhibitor of the mitochondrial atpase is present on the inner face of the inner membrane and is very sensitive to trypsin , it can be concluded that mitoplasts are mainly oriented as normal mitochondria while sonicated vesicles are mainly inverted .
C0230839
outer
C0026237
mitochondria