the fluorescence of the nbm or nsm coupled to the sulfhydryl group on the enzyme was quenched by binding to the enzyme of the substrate pyruvate the coenzyme thiamine diphosphate , the coenzyme analogue thiochrome diphosphate , the regulatory ligands acetyl-coa , gtp , and phosphoenolpyruvate , and the acetyl-coa analogue , ans .
C0039848
thiamine diphosphate
C0009235
coenzyme analogue