embedding glutaraldehyde ( ga ) -fixed nuclei in lowicryl k4m or k11m following dehydration by the progressive lowering of temperature ( plt ) method , or in k11m following spray freezing and freeze substitution ( fs ) , produces chromatin fibres that have , in situ , a diameter close to the in vivo state , and show internal structural details consistent with patterns of nucleosome packing previously observed only in preparations of isolated fibres .
C0008546
chromatin
C0028623
nucleosome