rna polymerase from escherichia coli was used in conjunction with labeled nucleosides as an autoradiographic reagent to study the availability of template in the chromatin of fixed nuclei and chromosomes sequential treatments of the tissues with acid and poly-l-lysine were used to compare the effect of these treatments on the availability of template with the previously reported effects on the in situ priming for escherichia coli dna polymerase acid treatment was found to increase the in situ activity of both enzymes , while poly-l-lysine strongly inhibited the in situ reactions mediated by rna and dna polymerases .
C0008546
chromatin
C0007610
nuclei