renin was completely purified from human kidney cortex employing a rapid three-step procedure which included homogenization and ammonium sulfate precipitation , aminohexyl-pepstatin affinity chromatography , and affinity chromatography using a synthetic octapeptide renin inhibitor ( h-77 ) with a reduced peptide bond ( -ch2-nh- instead of -co-nh- ) between leu5-leu6 , three kg of cortex dissected from 10 kg of human cadaver kidney yielded 1.7 +/- 0.5 mg of protein ( mean +/- s.e . for five procedures ) with a specific activity of 1094 +/- 166 goldblatt units/mg of protein and an overall recovery of 52 +/- 2 % .
C0022655
kidney cortex
C0022646
kidney