cytosol receptors were monitored by conventional procedures , and nuclear receptors were extracted from nuclei at 2 c using 0.5 m kcl in buffer containing glycerol and measured by ligand exchange during incubation with [ 3h ] progesterone ( p ) at 2 c for 24 h or [ 3h ] estradiol-17 beta ( e2 ) at 30 c for 1 h .
C0206588
nuclear receptors
C0007610
nuclei