immunoglobulin obtained from the serum of rabbits immunized with a highly purified preparation of estradiol-receptor complex from calf uterine nuclei has been shown to contain specific antibodies to the receptor protein ( estrophilin ) by four criteria : ( a ) precipitation of the radioactive steroid upon addition of goat antibody against rabbit gamma globulin to a mixture of the tritiated estradiol-receptor complex and the immunoglobulin , ( b ) adsorption of the estradiol-receptor complex by the immunoglobulin linked to sepharose , ( c ) adsorption of the estradiol-receptor complex in the presence of the immunoglobulin by staphylococcus aureus protein-a linked to sepharose , and ( d ) the ability of the immunoglobulin to increase the sedimentation rate of the estradiol-receptor complex .
C0003241
antibody
C0021027
immunoglobulin