the exposure of exponentially growing pseudomonas fluorescens p7 cells to heating at 36 c for 2 h in a defined medium , followed by cooling to 25 c and further incubation at this , the optimal growth temperature , resulted in the apparent death of approximately 99 % of the cells , as determined by their inability to form colonies on trypticase soy agar .
C0007634
cells
C0007587
death