we have used phorbol esters , such as 12-o-tetradecanoyl phorbol 13-acetate ( tpa ) , to study the actions of protein kinase c ( a tpa receptor ) on cytosolic free ca2+ concentrations [ ( ca2+ ] i ) and hormone secretion in rat pituitary cells ( gh cells ) , and to elucidate the role of diacylglycerol ( a protein kinase c activator ) in thyrotropin-releasing hormone ( trh ) action .
C1383501
cytosolic
C0007634
cells