a newly developed method that involves direct gas chromatography-mass spectrometry of sugars obtained by hydrolysis of proteins purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis ( sds page ) allows the identification and determination of small amounts of carbohydrates ( e.g. , 20 micrograms of glycoprotein containing a minimum of 0.1 % monosaccharide ) , even in the presence of relatively high amounts of impurities .
C0007004
carbohydrates
C0026492
monosaccharide