the steps are : extraction of the flour or dough and carboxymethylation of gsh , gel filtration on sephadex g-50 , chromatography on dowex 50 wx8 and after basic cleavage determination of the separated gssg with 5,5'-dithiobis ( 2-nitrobenzoic acid ) , separation of carboxymethylated gsh on dowex 1x8 and determination with 2,4,6-trinitrobenzenesulfonic acid .
C0061516
gssg
C0017817
gsh