finally , 10- ( 1-oxo-2-propynyl ) -estr-4-ene-3,17-dione , the proposed enzyme-generated affinity label and analog of the second intermediate ( 3,17-dioxoandrost-4-en-19-al ) in the enzymatic reaction , bound to the enzyme with an apparent ki of 12 microm and caused time-dependent inactivation with a pseudo-first order kinact of 5.35 x 10 ( -4 ) s-1 .
C0393003
oxo
C0393005
s-1