the cell layer at the interface was removed , washed in phosphate-buffered saline without ca2+ and mg2+ , and fixed with 90 % methanol , and intracellular hiv antigens were detected by indirect immunofluorescence with monoclonal antibodies to hiv antigens as the primary antibody and fluorescein isothiocyanate-conjugated goat anti-mouse immunoglobulin g f ( ab ' ) 2 antibody as the secondary antibody .
C0003250
monoclonal antibodies
C0003241
antibody