the ion pairs of amino acids with dodecyl sulphate were separated on a reversed-phase column ( beckman ultrasphere i.p . ) using a sequence of eluents that are prepared by mixing 0.2 m phosphoric acid ( containing 10 mm sodium dodecyl sulphate ) , 0.2 m sodium acetate ( ph 4.50 ; containing 10 mm sodium dodecyl sulphate ) and methanol . the amino acids commonly occurring in tissues , except taurine and related weakly basic amino acids , can be analysed at a rate of 95 min per sample at a sensitivity of less than 50 pmol per amino acid .
C0058632
dodecyl sulphate
C0037506
sodium