a selection strategy to obtain cells deficient in poly ( adp-ribose ) polymerase was developed based on the fact that treatment with high levels of n-methyl-n'-nitro-n-nitrosoguanidine results in sufficient activation of poly ( adp-ribose ) polymerase to cause nad and atp depletion leading to cessation of all energy-dependent processes and rapid cell death .
C0007634
cells
C0007587
cell death