hplc was carried out by using zorbax sil as the stationary phase and chloroform -- isopropanol -- acetic acid -- water -- sodium acetate buffer ( ph 4.5 ) ( 100:100:14:14:1 ) as the mobile phase , with a fluorescence spectrophotometric detector at an excitation wavelength of 470 nm and an emission wavelength of 585 nm .
C0000983
acetic acid
C0142785
sodium acetate