using an improved microgradient system with hydrophobic and hydrophilic layers of increasing density , a pure plastid pellet ( up to 90 % of total chloroplasts ) could be separated from an interphase of only slightly contaminated mitochondria ( 70 to 80 % of total mitochondria ) , and a cytoplasmic supernatant could be obtained within 60 seconds .
C0026237
mitochondria
C0010834
cytoplasmic