to investigate the role of glycosylation of t4-binding globulin ( tbg ) in the secretion of the protein , human hepatoma ( hep g2 ) cells were continuously labeled with [ 35s ] methionine or [ 3h ] mannose or pulse-chase labeled with [ 35s ] methionine in the absence or presence of 1 microgram/ml swainsonine , an inhibitor of golgi alpha-mannosidase ii and lysosomal alpha-mannosidase .
C1159339
secretion
C0007634
cells