with a combination of triton x-100 extraction or triton x-114 phase-separation techniques , bio-bead sm-2 treatment , dialysis , and gel filtration on sephadex g-200 or sephacryl s-300 , we obtained a sparingly soluble lipid-free protein demonstrating hydrolytic activity against triglycerides and negligible phospholipase or cholesteryl esterase activities .
C0020291
hydrolytic
C0031671
phospholipase