to confirm the in vivo observation that the n gene product of phi 80 , phi 80-pn , prevents termination of transcription at the tl1 region and is therefore a transcription antitermination factor ( tanaka and matsushiro 1985 ) , we demonstrated that phi 80-tl1 is a rho-dependent terminator , similar to lambda-tl1 , and that phi 80-pn has a transcription antitermination function at this site in an in vitro transcription system using a nucleic acid-free s-100 extract .
C0597600
termination
C0040649
transcription