to enhance the likelihood of identifying the gtp-binding proteins , two assays were employed to monitor gtp-binding activities : ( i ) guanosine 5'- ( 3-o- [ 35s ] thio ) triphosphate ( gtp gamma s ) -binding followed by rapid filtration and ii ) [ alpha-32p ] gtp-binding following sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotting onto nitrocellulose membranes .
C0086376
gtp-binding proteins
C1149035
gtp-binding