cytoplasmic free ca2+ ( ca2+i ) was chelated to 10-20 nm in the macrophage cell line j774 either by incubation with quin2 acetoxymethyl ester in the absence of external ca2+ ( di virgilio , f. , lew , p.d. , and pozzan , t. ( 1984 ) nature 310 , 691-693 ) or by loading [ ethyl-enebis ( oxyethylenenitrilo ) ] tetraacetic acid ( egta ) into the cytoplasm via reversible permeabilization of the plasma membrane with extracellular atp ( steinberg , t.h. , newman , a.s. , swanson , j.a. , and silverstein , ss.c . ( 1987 ) j. biol . chem . 262 , 8884-8888 ; di virgilio , f. , meyer , b.c. , greenberg , s. , and silverstein , s.c. ( 1988 ) j. cell biol . 106 , 657-666 ) .
C0010834
cytoplasm
C0007603
plasma membrane