the binding of radiolabeled [ 14c ] nadh and [ 14c ] nad+ to the e1 isozyme when measured by the sedimentation technique yielded linear scatchard plots with a dissociation constant of 17.6 microm for nadh and 21.4 microm for nad+ and a stoichiometry of ca . two coenzyme molecules bound per enzyme tetramer .
C0022173
isozyme
C0014442
enzyme