the method consists of treatment of intact spermatozoa with 1.25 mm edta , dispersion of these cells to a two-phase polymer system comprising 5.5 % 252-kd dextran and 4.2 % 20-kd polyethylene glycol compound and subsequent centrifugation at 12,000 x g for 30 min when the two phases separate out and membranes sediment at the interphase .
C0007634
cells
C0021798
interphase