the method is based upon : 1 ) separation of the vwf multimers by sds-agarose electrophoresis , 2 ) subsequent blotting of the vwf multimers onto nitrocellulose , 3 ) immunolocalization and visualization of the vwf pattern by the sequential incubation of the blot with primary vwf antiserum , peroxidase- or beta-galactosidase-conjugated secondary antibodies and a relevant chromogenic substrate .
C0020960
antiserum
C0003241
antibodies