the method provides sufficient structural preservation and spatial resolution for detailed functional investigation of complex but small-sized nervous systems when the original technique is modified as follows : ( i ) use of 3h instead of 14c as radioactive label , ( ii ) application of labeled deoxyglucose in concentrations close to physiological glucose levels rather than in trace amounts , ( iii ) stimulation for 4 -- 9 h after deoxyglucose application instead of 20 -- 45 min , ( iv ) subsequent preparation avoiding aqueous phases at all stages from fixation to autoradiography , and ( v ) plastic embedding of the tissue such that serial semithin sections of good structural preservation may be routinely cut .
C0027763
nervous systems
C0086045
concentrations