after incubation for 3 to 4 h with sperm , eggs were transferred to culture medium containing faf- or v-bsa , with or without amino acids ( glutamine , isoleucine , methionine , and phenylalanine ) , and the percentages of eggs cleaving to two cells were recorded after a further 20 h of incubation .
C0002520
amino acids
C0017797
glutamine