from changes observed in the integral and differential melting profiles , gathered via absorbance measurements at 260 nm as a function of ch3hgoh concentration , it is concluded that methylmercury at low concentrations ( 1 - 10 microm ) , and prior to its denaturating the double-helix of the chromosomal dna , interferes with the binding of dna by histones h3 and h4 which is followed , at methylmercury concentrations between 10 and 32 microm , also its interfering with the complexing of dna by the less basic histones h2a and dna denaturation , in absence of the histones , proceeds only at methylmercury concentrations above 32 microm under the experimental conditions given .
C1148673
binding
C0019647
histones h3