the majority of the binding activity appeared in the 30-45 % saturation fraction , which was then applied in sequence to an hgh-affinity column , to strong anion exchange ( sax ) chromatography ( a mono q column 0.01-0.2 m phosphate gradient ) , to a tsk phenyl hydrophobic interaction column ( hic ) ( 0.75-0 m sulphate gradient in 0.1 m phosphate ) and finally to a second sax step .
C0003075
anion
C0038720
sulphate