on the basis of earlier findings showing that h5hr1 ( hr1 ) is cold sensitive for transformation , a series of mutants were constructed so that they contained deletions or insertions in different sites of early region 1a ( e1a ) to ascertain : ( i ) whether the cold-sensitive phenotype of hr1 was the result of the identified single-base pair deletion of nucleotide 1,055 or due to a missense mutation at another site and ( ii ) what region and how much of the e1a 51-kilodalton protein is actually required to produce cell transformation .
C1709313
e1a
C0101583
e1a