to monitor membrane permeabilisation we have used two fluorescent dyes , ethidium which stains the nucleus , and tma-dph which stains the cytosolic surfaces of intracellular membranes following entry into the cells permeabilisation by atp is not affected by the metabolic status of the cells , and is maintained at temperatures as low as 8 degrees c .
C0021868
intracellular membranes
C0007634
cells