after coating round-bottomed wells of polyvinyl chloride with porcine monocomponent insulin , 50 microliters of serum from either insulin-treated diabetics or non diabetic controls was added to the wells , followed by the addition of 125i-protein a , and then the radioactivity of the bound 125i-protein a on the wells was counted .
C0724601
porcine
C0021641
insulin