on the basis of the zein content in acidic and basic amino acids , and of the respective pi 's exhibited in ief ( mostly in the ph range 7-8 ) it has been calculated that at least 90 % of the glutamic and aspartic acids ( ? 52 residues out of a total of ? 190 ) are present as asparagine and glutamine .
C0301714
basic amino acids
C0017797
glutamine