specific binding was not inhibited by a large excess of antigen in the media ( 5000-fold greater than the amount of antigen associated with the macrophages ) either soluble or bound to sepharose beads , or by coating the antigen-pulsed macrophags with antibody to the exogenous antigen , by reacting a second layer of antibody to the heterologous antibody , or by haptenating the antigen and treating the hapten-antigen macrophage complex with excess anti-hapten antibody .
C0003241
antibody
C0003313
antigen