a method of rapid freezing in supercooled freon 22 ( monochlorodifluoromethane ) followed by cryoultramicrotomy is described and shown to yield ultrathin sections in which both the cellular ultrastructure and the distribution of diffusible ions across the cell membrane are preserved and intracellular compartmentalization of diffusabler ions can be quantitated .
C0007634
cellular
C0007603
cell membrane