for the active enzyme-phospholipid complex in the absence of ligands , we previously found that reaction with n-ethylmaleimide ( at 5 mol/mol of enzyme subunit ) resulted in progressive loss of enzymic activity with an inactivation stoichiometry of 1 equiv of sulfhydryl derivatized per mole of enzyme and a maximum derivatization of 2 equiv [ latruffe , n. , brenner , s. c. , & fleischer , s. ( 1980 ) biochemistry 19 , 5285-5290 ] .
C0026741
enzyme
C0014442
enzyme