the hepatic portal vein endothelial cell ( hpvec ) derived from intrahepatic portal veins by surgically , have been propagated , as outgrowth cultures in rpmi-1640 medium with 10 % fetal bovine serum , on permeable collagen membranes ( koken , tokyo ) containing mainly type i collagen , covered with a solubilized tissue basement membrane ( matrigel , collaborate res. , inc. , bedford ma ) involving type iv collagen , laminin and proteoglycan .
C0004799
tissue basement membrane
C0009333
type iv collagen