therefore , after ligation of all side branches , vessel segments , prepared either with or without endothelium , were cannulated and arranged in two systems ( with two segments each ) in a serial manner ( system i : endothelium-denuded vessel followed by an endothelium-denuded segment ) and perfused with tyrode 's solution ( constant flow 20 ml/min ) .
C0023690
ligation
C0005847
vessel