sustained growth in primary culture of vaginal epithelial cells from ovariectomized adult balb/ccrgl mice embedded within or seeded on collagen gel matrix was achieved in a serum-free medium composed of ham 's f-12 medium/dulbecco 's modified eagle 's medium , 1:1 ( vol/vol ) , supplemented with insulin , bovine serum albumin fraction v , epidermal growth factor , cholera toxin , and transferrin .
C0007595
growth
C0009325
collagen