an oligoribonucleotide ( 35-mer ) that mimics the alpha-sarcin and the ricin region of eukaryotic 28 s rrna was transcribed in vitro from a synthetic template with t7 rna polymerase and was used to test whether the specificity of the hydrolysis by the toxins was retained . alpha-sarcin , at a low concentration , cleaved a single phosphodiester bond on the 3 ' side of a guanosine residue in the synthetic oligomer that corresponds to g-4325 in 28 s rrna , the site of action of the toxin in intact ribosomes .
C0035701
rrna
C0035553
ribosomes