cd3+ wt31- t cells were sorted from peripheral blood of a normal healthy donor by a facs iv and cloned by limiting dilution in the presence of a phorbol ester ( tetradecanoyl phorbol acetate , tpa ) , calcium ionophore ( ionomycin , io ) , interleukin-2 ( il-2 ) , allogeneic cells , and phytohemagglutinin ( pha ) .
C0031586
phorbol ester
C0039654
tetradecanoyl phorbol acetate