nuclear extracts , prepared by incubating nuclei isolated from sv40-infected cv-1 cells in a hypotonic buffer to release both sv40 replicating and mature chromosomes , were either centrifuged to separate the total sv40 nucleoprotein complexes from the soluble nucleosol or fractionated on sucrose gradients to provide purified sv40 replicating chromosomes .
C0007610
nuclei
C0007634
cells