the assay involves the conversion of the enantiomers of both fenfluramine and norfenfluramine into their corresponding diastereomeric amide derivatives by an acylation reaction with n-heptafluorobutyryl-s-prolyl chloride under schotten-baumann conditions prior to gas chromatographic separation on an achiral polar ov-225 capillary column .
C0015827
fenfluramine
C0028362
norfenfluramine