pretreatment of isolated rat liver plasma membranes by washing with nahco3 buffer or by exposure to the chelator ethyleneglycol bis ( beta-aminoethyl ether ) -n , n , n ' , n'-tetraacetic acid ( egta ) with or without the ionophore a23187 , produced a decrease in the sensitivity of adenylate cyclase ( atp pyrophosphate-lyase ( cyclizing ) ec 4.6.1.1 ) to subsequent stimulation by naf or guanosine 5'- ( beta-gamma-imino ) triphosphate ( gpp ( nh ) p ) .
C0007603
plasma membranes
C1382029
atp