we employed two new methods : one is promotion of endocytosis by incubating the epidermal sheet in tissue culture medium with okt6 at 4 degrees c and further incubation without okt6 at 37 degrees c , and the other is the use of saponin to facilitate penetration of the secondary antibody through the cytomembrane of lc .
C0014520
epidermal
C0023005
lc