we describe a new radioligand assay for determining biotin in biological fluids by using a mixture of n- [ beta- ( 4-oh-3-125i-phenyl ) ethyl ] - and n- [ beta- ( 4-oh-3,5-di-125i-phenyl ) ethyl ] biotinamides as radiotracer , avidin as a binding protein , and an avidin double-antibody as a separation reagent .
C0005889
fluids
C0003241
antibody