ca2+ transport by sarcoplasmic reticulum vesicles was examined by incubating sarcoplasmic reticulum vesicles ( 0.15 mg/ml ) at 37 degrees c in , either normal medium that contained 0.15 m sucrose , 0.1 m kcl , 60 microm cacl2 , 2.5 mm atp and 30 mm tes at ph 6.8 , or a modified medium for elimination of adp formed from atp hydrolysis by including , in addition , 3.6 mm phosphocreatine and 33 u/ml of creatine phosphokinase .
C1510699
atp
C0010287
creatine phosphokinase