calibration of the percent modification with [ 14-c ] aaf and analysis of the size of the rna product by double isotope labeling and polyacrylamide gel electrophoresis support the following mechanism of transcription inhibition : most of the aaf residues bound to the coding strand of the dna cause premature termination of transcription , at or near the site of modification , with release of rna polymerase .
C0040649
transcription
C0035681
rna polymerase