affinity columns for the separation of rabbit antibodies ( abs ) to the purified nicotinic acetylcholine receptor ( acchr ) from torpedo californica electroplax were constructed in two ways : ( 1 ) by direct cross-linking of purified acchr to cyanogen bromide activated sepharose 4b , and ( 2 ) by first cross-linking a purified curarimimetic neurotoxin to cyanogen bromide activated sepharose 4b , subsequently saturating with acchr , and finally cross-linking the toxin to noncovalently bound receptors with bisimidate cross-linkers such as dimethyl suberimidate ( dms ) .
C0034830
nicotinic acetylcholine receptor
C0034792
acchr