also cells grown on yeast nitrogen base without amino acids with casamino acids degraded the hegf after cell growth as opposed to a yeast extract , peptone , and dextrose ( yepd ) medium , which elicited no measurable extracellular proteolysis of the hegf . alpha-factor directed production kinetics of hegf on the yepd medium were growth associated , secretion limitations and extracellular degradation were negligible , and the hegf was nearly 100 % selectively secreted .
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the
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secreted