using a lineweaver-burk plot ( double reciprocal ) , it was found that the michaelis-menten constant ( km ( for the immobilized enzyme was ( 4.0 +/- 0.9 ) x 10 ( -3 ) m and that of the free enzyme was ( 3.0 +/- 0.2 ) x 10 ( -3 ) m .
C0014444
immobilized enzyme
C0014442
enzyme