continuous observation of living malignant cells ( l1210 ) in a specially devised glass observation chamber with an inverted microscope made it possible to classify various cellular morphological features induced by antitumor agents into the following four stages : 1 ) an initial stage consisting of cytoplasmic granulation and coloring , 2 ) an intermediate stage of shrinkage of nuclei , increases of vacuoles and cytoplasmic budding , 3 ) a determinate stage of cell ballooning and 4 ) the terminal stage ( cell ghost ) .
C0010834
cytoplasmic
C0007634
cell