by submitting urine to the following operations : ( a ) extraction of the 17-oxosteroid conjugates into chloroform ( in the presence of pyridinium sulphate ) , ( b ) solvolysis ( in the absence of acid ) of the steroid sulphates , ( c ) separation of the steroid glucosiduronates , ( d ) hydrolysis of the glucosiduronates , ( e ) oxidation with tert.-butyl chromate of the liberated steroids and ( f ) analysis of the final extracts by gas-liquid chromatography , the 17-oxosteroid conjugates are converted into and measured as androstanediones and androstane- ( ene ) triones .
C0038317
steroids
C0002854
androstane