we investigated whether occupation of these binding sites evokes the release of hydrolytic enzymes from mouse peritoneal macrophages cultured for up to 48 h. ac-ldl at concentrations ranging from 25-250 micrograms protein/ml was noted to promote in a dose-dependent fashion secretion of the neutral proteinase elastase ( ec 3.4.21.37 ) and the lysosomal acid hydrolases n-acetyl-beta-glucosaminidase ( ec 3.2.1.30 ) , beta-glucuronidase ( ec 3.2.1.31 ) , beta-galactosidase ( ec 3.2.1.23 ) , alpha-mannosidase ( ec 3.2.1.24 ) and cathepsin d ( ec 3.4.23.5 ) .
C0020291
hydrolytic
C0020289
hydrolases