addition of thioglycolate and deae-sephadex chromatography were used to analyze the cleavage of the c ( 3 ' ) -o-p bond 3 ' to ap ( apurinic/apyrimidinic ) sites in dna and to distinguish between a mechanism of hydrolysis ( which would allow the nicking enzyme to be called 3 ' ap endonuclease ) or beta-elimination ( so that the nicking enzyme should be called ap lyase ) .
C0020291
hydrolysis
C0140145
ap lyase