the binding affinity expressed by the microscopic equilibrium constant ( ki ) ( yuhasz , et al. , biochemistry 26 , 2334-2342 ( 1987 ] of the immunizing hapten , o6-ethyl-2'-deoxy-guanosine ( *g ) and eight structural analogs , were analyzed by a nitrocellulose affinity filter assay ( nafa ) and radioimmunoassay ( ria ) for each mab to determine the protein-hapten interaction between the epitope and the binding cavity .
C0018593
hapten
C0003316
epitope