the monovalent-cation-specific , carboxylic acid ionophores , nigericin and monensin , were utilized to produce transmembrane proton gradients in cells that were allowed to generate intracellular carboxyfluorescein in a preliminary incubation , then resuspended in media buffered at the same ph as the sperm cytosol but of varying cation composition .
C0007634
cells
C1383501
cytosol