the total work involved ( i ) the synthesis of a 126-nucleotide-long bihelical dna corresponding to a known precursor to the tyrosine suppressor transfer rna , ( ii ) the sequencing of the promoter region and the distal region adjoining the c-c-a end , which contained a signal for the processing of the rna transcript , ( iii ) total synthesis of the 207 base-pair-long dna , which included the control elements , as well as the eco r1 restriction endonuclease specific sequences at the two ends , and ( iv ) full characterization by transcription in vitro and amber suppressor activity in vivo of the synthetic gene .
C0012854
dna
C0598312
synthesis