we have utilized competition studies , saturation analysis , sucrose-density-gradient sedimentation and deae-cellulose chromatography to demonstrate that long bones of vitamin d-depleted newborn rats contain cytoplasmic and possibly nuclear receptors that bind 24,25-dihydroxycholecalciferol with specificity and high affinity ( kd = 1.79 nm ) .
C0010834
cytoplasmic
C0206588
nuclear receptors