based on the selective inhibition of glutamate release in cerebellar granule cells in primary cultures by the aspartate aminotransferase inhibitor , aminooxyacetic acid , and by the ketodicarboxylate carrier inhibitor , phenylsuccinate , a novel model for synthesis of transmitter glutamate is suggested : glutamate is formed from glutamine in the mitochondrial intramembrane space by phosphate-activated glutaminase , transported across the inner membrane in exchange with aspartate , transaminated in the matrix to alpha-ketoglutarate , which via the ketodicarboxylate carrier is transferred to the cytoplasm , and transaminated to form transmitter glutamate .
C0026237
mitochondrial
C0010834
cytoplasm