incubations in vitro of ga1 , labeled with 3h in the terminal d-galactopyranosyl group , with nonradioactive cmp-neunac in the presence of homogenates of c21 rat brain glial cells , nie mouse neuroblastoma cells , 3t3 mouse fibroblasts , sv 40-transformed 3t3 cells , chick embryo fibroblasts , rous sarcoma virus-transformed chick embryo fibroblasts , and 9-day old rat brain resulted in all cases in the formation in high yield of gm1b , in which the neuraminidase-labile neunac group is linked at o-3 of the terminal d-galactosyl residue , as shown by permethylation studies .
C0007634
cells
C0016030
fibroblasts