cells of a fractionated dispersion ( 0.25 % trypsin-pbs , 10 degrees c ) of pituitaries formed monolayer ( fibroblast- and epithelial-like-cells ) at 25 degrees c using eagle 's minimal essential medium ( mem ) supplemented with 10 % fetal calf serum and non essential amino acids .
C0007634
cells
C0016030
fibroblast