the identity of type-iii collagen was established on the basis of ( a ) insolubility in 1.7 m-nac1 at neutral ph , ( b ) behaviour of this collagen fraction on gel electrophoresis under reducing and non-reducing conditions , ( c ) amino acid analysis showing a hydroxyproline/proline ratio greater than 1 , and ( d ) profile of cnbr peptides on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis showing a peak characteristic for type-iii collagen containing peptides alpha1 ( iii ) cb8 and alpha1 ( iii ) cb3 .
C0020388
hydroxyproline
C0033382
proline