supplementation of the basal medium with a combination of five groups of factors ( 5fs ) , such as eight amino acids ( ala , arg , gly , ile , met , phe , pro , and trp ) , two unsaturated fatty acids ( linoleate and oleate ) , a protease inhibitor ( aprotinin ) , three vitamins ( a , c , and e ) , and five trace elements ( mn , fe , cu , zn , and se ) , improved the hepatocyte survival under the serum-free conditions in the presence or absence of barbiturates .
C0002520
amino acids
C0017890
gly