quiescent cultures of normal fetal rat calvarial bone cell populations ( rc i and rc iv ) and human fibroblasts were incubated with 1.0 ng/ml tgf-beta and the conditioned culture media were processed individually to separate collagenase and 72 kda-progelatinase from timp , the tissue inhibitor of matrix metalloendoproteinases , using mini-columns of heparin- and gelatin-sepharose .
C0086418
human
C0080222
1