1 the effects of potassium depolarization and preganglionic nerve stimulation on the metabolism of [ ( 3 ) h ] -choline in the isolated superior sympathetic ganglion of the rat have been studied.2 when unstimulated ( resting ) ganglia were incubated for 10 min with a low concentration ( 0.1 mum ) of [ ( 3 ) h ] -choline ( high affinity uptake ) , approximately 75 % of the accumulated radioactivity was present as [ ( 3 ) h ] -phosphorylcholine , 11 % was [ ( 3 ) h ] -acetylcholine ( [ ( 3 ) h ] -ach ) and the remainder was unchanged [ ( 3 ) h ] -choline.3 depolarization of the ganglia with k ( 46 mm ) before their incubation with [ ( 3 ) h ] -choline , increased [ ( 3 ) h ] -choline uptake by 70 % and increased [ ( 3 ) h ] -ach synthesis by more than 700 % , so that [ ( 3 ) h ] -ach represented almost 50 % of the total radioactivity recovered .
C0031716
phosphorylcholine
C0008405
choline