the two phosphoproteins were identified as camp-pde or a closely related molecule ( in the case of the 44-kda species , perhaps a proteolytic fragment ) since ( i ) amounts of 32p in the immunoprecipitated 135-kda protein paralleled enzyme inactivation , ( ii ) prior incubation of the anti-camp-pde with the pure rat or bovine enzyme selectively blocked the immunoprecipitation of the phosphoproteins , ( iii ) 135- and 44-kda proteins reacted with the anti-camp-pde on western immunoblots , and ( iv ) the two phosphoproteins copurified with camp-pde activity through deae-sephacel chromatography and were isolated by highly selective affinity chromatography on cilostamide-agarose .
C0033684
proteins
C0031689
phosphoproteins