treatment of cells with 50 ng of tpa per ml followed by resolution of the membrane and cytosol in the presence of ethylene glycol-bis ( beta-aminoethyl ether ) n , n , n ' , n'-tetraacetic acid ( egta ) promoted an apparent translocation of both 78- and 80-kda proteins from the cytosol to the membrane fraction .
C0007634
cells
C1383501
cytosol