this conclusion is supported by the following observations : ( 1 ) essentially identical growth rates were observed in eagle 's minimum essential medium ( mem ) 3 in which the glucose concentration was either 5.5 mm or was maintained between 25 and 40 micrometer , ( 2 ) the total glutamine utilization by fibroblasts increase at least 30 % in medium with 25 micrometer to 70 micrometer glucose compared to medium with 5.5 mm glucose , while the rate of glutamine-1 or 5-14c oxidation to co2 increased 5-fold as the glucose concentration was decreased to zero , ( 3 ) 2 mm glutamine inhibited glucose-6-14c oxidation by 88 % and stimulated glucose-1-14c by 77 % in log phase cells and ( 4 ) glutamine oxidation in normal medium contributed approximately 30 % of the energy requirement of human diploid fibroblasts .
C0007634
cells
C0016030
fibroblasts