this procedure had several advantages as compared to treatments with nonionic or ionic detergents : ( i ) the solubilized enzyme was stable at 4 c , ( ii ) more than 80 % of the replicase could be solubilized without loss of total enzyme activity , ( iii ) the replicase was rather selectively released resulting in a two- to threefold increase in specific activity per se , and ( iv ) most of the green color from chloroplast fragments present in the crude replicase fraction remained membrane bound resulting in only slightly colored preparations of solubilized enzyme .
C0184661
procedure
C0087111
treatments