a rapid method for assembly of dna from synthetic oligodeoxynucleotides has been developed which involves separate ligation of top- and bottom-strand oligonucleotides followed by filling in 3'-ends of the duplex formed , blunt end cloning into a specialized vector pbbv , and recovery of the synthetic dna from the recombinant plasmid by means of restriction nuclease bbvii .
C0028948
oligodeoxynucleotides
C0028953
oligonucleotides