the stimulatory and inhibitory regulatory components of adenylyl cyclase ( ns and ni ) , purified to apparent homogeneity without the use of regulatory ligands such as mg , naf , and guanyl-5'-yl imidodiphosphate , were tested for gtpase activity by incubating them with [ gamma-32p ] gtp and measuring 32pi liberation using a charcoal adsorption assay to separate hydrolyzed from nonhydrolyzed radioactivity .
C0018366
guanyl-5
C0018353
gtp