this line was originally selected by the limiting dilution of mltc cells from rl male 1-immune ( balb/c x c57bl/6 ) f1-nu/+ ( cb6f1-nu/+ ) spleen cells ( 500 cells/well ) in the presence of 5 % rat tcgf , 2000 rads-irradiated normal cb6f1-nu/+ spleen cells as the feeder cells , and 10,000 rads-irradiated rl male 1 tumour cells as the stimulator .
C0699040
limiting
C0007634
cells