because , for most subjects , the quantity of ige in culture supernatants from 7-day cultures of living cells was the same as that derived from cells which were either frozen and thawed prior to culture , or briefly exposed to ph 3.7 buffer , the majority of ige antibody in supernatants from allergic subjects , and of total ige in supernatants from all subjects , was apparently not newly synthesized , but was preformed , and appeared to be largely derived from the cell surface .
C0007634
cells
C0699040
cell