the effect of formate and nitrate on increasing the residence time of creatine in the mnadp-creatine-enzyme complex as determined by nmr provides evidence that the complexes observed by nmr are identical with those involved in the catalytic mechanism , since a parallel effect of formate and nitrate is observed in the kinetics of the enzymatic reaction , where the dissociation constant of creatine from the abortive quaternary complex decreases in the presence of the anions as had been determined from their inhibition of the forward reaction ( milner-white , e.j. , and watts , d.c. ( 1971 ) biochem . j . 122 , 727-740 ) .
C0028125
nitrate
C0003075
anions