to construct a nondefective ad5 bearing the 2.8-kb dna fragment of hepatitis b virus ( hbv ) at this site , we deleted the 2.1-kb nonessential e3 fragment from cloned dna covering the right one-fourth of the ad5 genome ( 76 to 100 map units ) , inserted the hbv dna into this site , ligated the recombinant dna to the rest of the ad5 genome , and transfected the ligated dna into human embryo kidney cells .
C0012931
recombinant dna
C0012854
dna