the vectors used in this system contain a p1 packaging site ( pac ) to package vector and cloned dna into phage particles , two p1 loxp recombination sites to cyclize the packaged dna once it has been injected into a strain of escherichia coli containing the p1 cre recombinase , a kanr gene to select bacterial clones containing the cyclized dna , a p1 plasmid replicon to stably maintain that dna in e. coli at one copy per cell chromosome , and a lac promoter-regulated p1 lytic replicon to amplify the dna before it is reisolated .
C0007634
cell
C0086860
regulated