the orit site was subcloned from ped806 on a haeii fragment including a haeii-bg/ii segment of f dna approximately 385 base pair ( bp ) long into the 2.25 kilobase ( kb ) vector plasmid ped825 , giving ped822 . ped822 was fully proficient for orit function , and recircularised in recipient cells by a reca- and tra-independent orit -specific ligation/recombination event .
C1419240
reca
C0034865
recombination