the activity of alpha-type tgfs was examined by soft agar colony formation of nrk49f cells and inhibition of egf-binding to a 431 cells since tgf alpha has sequence homology with egf and binds to egf receptors on the cell membrane , while the activity of beta-type tgfs was examined by soft agar colony formation of 3t3 cells and nrk 49 f cells with the addition of egf .
C0007634
cells
C0007603
cell membrane