an aminoglycoside-3'-phosphotransferase i catalyzing phosphorylation of some aminoglycoside antibiotics with the 3'-hydroxyl group has been purified from the cells of aminoglycoside resistant strain e. coli 182 by competitive affinity chromatography on neomycin-sepharose and gel-filtration on sephadex g-100 .
C0002556
aminoglycoside
C0027603
neomycin