2 ) that , after patch excision , the active state of the ligand-stimulated channel is dependent on gtp in the bath , is abolished by treatment of the cytoplasmic face of the patch with activated ptx and nad+ , and after inactivation by ptx , is restored in a gtp-dependent manner by addition of a nonactivated human erythrocyte ptx-sensitive g protein , and 3 ) that the 55-picosiemens k+ channel can also be activated in a ligand-independent manner with guanosine [ gamma-thio ] triphosphate ( gtp gamma s ) or with mg2+/gtp gamma s-activated erythrocyte g protein .
C0018353
gtp
C0079469
gtp gamma s