using a class of bis-benzimidazole dyes , hoechst 33258 and 33342 and a multiparameter computer-controlled cell sorter , we have been able to stain and separate living cells in the g1 , s , and g2+m phases of the cell cycle and to continue their growth in tissue culture with high retention of viability ( greater than 90 % ) and no increase in heteroploidy .
C0007634
cells
C0079396
g2