in the current studies we tested whether gs redistributes within cells by treating s49 lymphoma cells with the beta-adrenergic agonist isoproterenol , then separating cytosol and crude membrane fractions ( defined as pellet and supernatant , respectively , after centrifugation for 1 hr at 150,000 x g ) , and assaying fractions for the alpha subunit of gs ( alpha s ) using a competitive elisa and reconstitution techniques .
C0007634
cells
C1383501
cytosol