human skin fibroblasts were cultured under conditions optimized for collagen synthesis , and the effects of ascorbic acid on procollagen production , proline hydroxylation and the activity of prolyl hydroxylase were examined in cultures . the results indicated that addition of ascorbic acid to confluent monolayer cultures of adult human skin fibroblasts markedly increased the amount of [ 3h ] hydroxyproline synthesized .
C0033382
proline
C0020388
hydroxyproline