ethanol or aldehyde fixed muscles were passed to water , postfixed 6-24 h with mercuric acetate ( 5 % w/v in 1 % acetic acid ) , washed with 1 % acetic acid and distilled water , stained 12-24 h at 43 degrees c with ammoniacal silver nitrate ( 60 % w/v ) and washed in 10 % sodium sulfite ( three changes ) and water .
C0790233
distilled water
C0043047
water