the cytosol was acidified either by incubating the cells with acetic acid , by incubating them with ammonium chloride which was subsequently removed in the presence of amiloride to prevent ph regulation by the na+/h+ exchanger , or by incubating the cells in isotonic kcl in the presence of nigericin and valinomycin .
C1383501
cytosol
C0007634
the cells