lysosomal membrane fractions were prepared from lysosomes of mouse liver by freeze-thawing in a hypotonic buffer : 54 % of beta-glucosidase [ ec 3.2.1.45 ] in lysosomes was associated with the membrane fractions , whereas 96 % of beta-glucuronidase [ ec 3.2.1.31 ] was recovered in the soluble fractions of lysosomes . beta-glucosidase was solubilized by ph 9.5 treatment or by triton treatment of membranes .
C0230812
lysosomal membrane
C0024369
lysosomes