first , serum or plasma is incubated with the antibody-coated bead and biotin-labeled antibodies , simultaneously , at 45 degrees c for 1 h ( `` '' stat '' '' procedure ) , 3 h ( `` '' standard '' '' procedure ) , or 18 h ( `` '' overnight '' '' procedure ) , during which hbsag forms a complex with the solid-phase antibody and the biotinylated antibodies .
C0229671
serum
C0032105
plasma