this is applied to a sep-pak column , and stepwise elution carried out under gravity with 0.1 divided by isopropanol in hexane ( neutral lipids ) , 1 % isopropanol in hexane ( d3 ) , 3 divided by isopropanol in hexane ( 25ohd3 ) , 3.125 divided by ethanol in dichloromethane ( 24,25 ( oh ) 2d3 ) and 50 divided ethanol in toluene ( 1 , 25 ( oh ) 2d3 ) .
C0022237
isopropanol
C0001962
ethanol