in serum-free medium [ ham 's f-12 : eagle 's minimum essential medium ( 1:1 , v/v ) containing 0.2 % bovine serum albumin ] , testosterone [ 10 ( -8 ) m ] also markedly stimulated the growth of spindle-shaped sc-3 cells , and epidermal growth factor ( 1 ng/ml ) enhanced the growth-stimulative effect of testosterone , whereas 17 beta-estradiol ( 10 ( -8 ) -10 ( -6 ) m ) in the absence or presence of epidermal growth factor had no growth-stimulative effect on sc-3 cells .
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growth of
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cells