before isoproterenol treatment only the competitive elisa was sensitive enough to detect cytosolic alpha s , whereas at later time points ( greater than or equal to 30 min ) the presence of alpha s in the cytosol was confirmed by both immunoblotting and by reconstitution of adenylyl cyclase [ atp pyrophosphate-lyase ( cyclizing ) , ec 4.6.1.1 ] in gs-deficient membranes derived from cyc-s49 cells .
C1383501
cytosol
C0007634
cells