to confirm that p14 is made in reovirus-infected cells , determine its intracellular location , and generate sufficient amounts of the polypeptide to begin an analysis of its presumptive role in the virus life cycle , the p14 coding sequence of an s1 cdna clone was subcloned into the ecori site downstream of the lambda pl promoter in the bacterial expression vector , pev-vrf1 .
C0007634
cells
C0079941
coding sequence