salivary gland antigen ( 200 ng ) eluted from the immunoaffinity column by 0.2 m na2co3 , ph 11.3 , and emulsified with incomplete freund 's adjuvant conferred a significant level of tick rejection ( 24 % , p less than 0.001 ) on naive guinea pigs compared with that seen in controls , but less than ( p less than 0.01 ) the level of immunity conferred by crude salivary gland antigen ( 380 micrograms ) .
C0020964
immunity
C0003320
antigen