when l5/a10 myoblast are cultured in f14 supplemented with 10 % fetal calf serum the cells grow with a division time of 12 h and reach confluency at a cell density of approximately 2.4 x 10 ( 5 ) cells per cm2 , without undergoing differentiation , characterized , morphologically , by formation of multinucleated fibers , and biochemically , by the synthesis of muscle specific proteins such as creatine phosphokinase or myokinase .
C0007634
cells
C0007589
differentiation