after incubation with t3 , intact nuclei were obtained by centrifugation after lysing the cells in a sucrose-tris-hcl buffer containing 0.2 % saponin and then adding triton x-100 ( final concentration 0.125 % ) to the lysed mixture to reduce nonspecific binding to less than 10 % of total binding .
C0007610
nuclei
C0007634
the cells