requirement for bsa was eliminated by ( i ) removing much of the apparent toxicity of free fatty acids in tween 80 on an anion exchange column , ( ii ) decreasing extended lag periods observed from small inocula by incorporation of pyruvate into the medium , ( iii ) the addition of acetate to permit full utilization of substrate fatty acids in tween 80 , and ( iv ) the addition of glycerol to decrease generation times .
C0021966
i
C0003075
anion