since the ice-nucleation temperature of mouse ova in the cryoprotectants dmso and glycerol is usually below -30 degrees c , the arrhenius form of the water-loss equation was used to compute the extent of supercooling in ova cooled at rates between 1 and 8 degrees c/min and the consequent likelihood of intracellular freezing .
C0020746
ice
C0043047
water