we purified human epidermal growth factor ( hegf ) and hegf fragments from a benzoic acid precipitate of the materials not adsorbed to silica gel in 330 liters of human urine by a relatively brief , simple , and efficient method employing sequential batch absorption to and stepwise elution from cm-cellulose and deae-cellulose , bio-gel p-10 chromatography in 50 mm hcl , and three reverse-phase hplc steps for final resolution and purification of hegf components .
C0007648
cellulose
C0011050
deae-cellulose