treat the eye as follows : 1 ) 50 % pyridine for at least 16 hr , 2 ) distilled water 3 -- 4 hr , 3 ) 20 % h2o2 until the eye is a light brown , 4 ) 95 % ethanol overnight , 5 ) 1.5 % agno3 for 2 -- 6 days at 37 c , 6 ) in water , remove the vitreous , then direct 0.25 % pyrogallic acid in 1.25 % formalin against the retina for 2 -- 5 secs until the optic fibers are reduced to a coffee-copper color ( 1 -- 4 minutes ) , 7 ) dissect the retina and mount flat on a glass slide , 8 ) cover with glycerin , apply a coverslip , and fix in place with nail polish .
C0790233
distilled water
C0043047
water