these results are in excellent agreement with predictions based on an equation derived by schellman ( ( 1975 ) biopolymers 14 , 999-1018 ) to take into account the effect of ligand binding on the melting temperature of a protein . delta t 1/2 = tt0r divided by delta hd ln ( 1 + kb [ s ] ) where t and t0 are t1/2 values in the presence and absence of ( glcnac ) 3 , delta hd is the enthalpy of denaturation in the presence of ( glcnac ) 3 , kb in the equilibrium constant for the binding of ( glcnac ) 3 to lysozyme , and [ s ] is the free concentration of ( glcnac ) 3 .
C1517880
ligand binding
C1413227
t1