using protein-free modified tyrode 's medium ( tlp-pva ) containing 10 mm lactate ( l ) , 0.1 mm pyruvate ( p ) , and amino acids ( phe , ile , met and gln ) , we found that development of hamster 8-cell embryos to blastocysts was supported better in the absence of glucose than in medium containing ( standard ) 5 mm glucose ( 88.1 % and 50 % , respectively ) .
C0002520
amino acids
C0017797
gln