these include formaldehyde vapor at 80 degrees c for 1 hour , 5 % paraformaldehyde solution at room temperature followed by heating to 80 degrees c for 15 min , and sucrose-phosphate buffer-glyoxylic acid solution either for 3 seconds at room temperature followed by heating to 80 degrees c for 15 min or 15 min at 60 degrees c in the solution .
C0016564
formaldehyde
C0070066
solution