the kinase complexed with tubulin was identical to the previously purified kinase as judged by several criteria including ( 1 ) subunit molecular weights , ( 2 ) isoelectric points , ( 3 ) autophosphorylation characteristics , ( 4 ) calmodulin binding properties , ( 5 ) kinetic parameters of tubulin phosphorylation , ( 6 ) phosphoamino acid phosphorylation sites on alpha- and beta-tubulin , and ( 7 ) identical subunit 125i-tryptic peptide maps .
C0031727
kinase
C0031715
phosphorylation