the complex was dissociated in a high-ionic-strength buffer containing 6 m urea and then fractionated by gel filtration and anion-exchange fast protein liquid chromatography into four sulfated sialoglycoprotein components , designated rs-a-1q1 , rs-a-1q2 , rs-a-1q3 , and rs-a-2 , with rs-a-1q2 being polydisperse through differential glycosylation of the polypeptide backbone .
C0003075
anion
C0038720
sulfated