paraffin sections are brought to water , mordanted for 1 hr in bouin 's fixative previously heated to 56 c , cooled while still in bouin 's , rinsed in tap water until sections are colorless , rinsed in distilled water , treated with 5 % aqueous tannic acid 5-20 min , rinsed in distilled water 30 sec or less , treated with 1 % aqueous phosphomolybdic acid 10-15 min , rinsed 30 sec in distilled water , rinsed in methanol , stained 1 hr in a saturated solution of amido black or phloxine b in 9:1 methanol : acetic acid , rinsed in 9:1 methanol : acetic acid , dehydrated , cleared and mounted .
C0043047
water
C0790233
distilled water