the ability of saccharomyces cerevisiae to transport d-galactose and related sugars with an axial hydroxyl group at c-4 , acquired by induction with d-galactose , was lost either by exposing early exponential-phase cells to an osmotic shock involving incubation in 0.6m nac1o4 , 0.66m sucrose and 1mm histidine and transfer to 5mm tris-hc1 with 2mm dithiothreitol , or simply by transferring them to distilled water .
C0242209
sugars
C0038636
sucrose