optimal procedures for minimizing the chance of negative staining of chat-containing axon terminals consisted of perfusion with a mixture of 4 % paraformaldehyde and 0.2 % glutaraldehyde for 3 min followed by a fixative containing only 4 % paraformaldehyde for 10 min , and reaction with the anti-chat antibody for 2 days at 37 c .
C0008407
chat
C0001068
anti-chat