incubation of the cells with sulfosuccinimidyl ( hydroxyphenyl ) propionate ( sulfo-shpp ) , a water-soluble derivative of the bolton-hunter reagent results in the coupling of hydroxyphenyl groups to free amino groups of cell surface proteins and these groups are then vectorially radioiodinated using 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril ( iodogen ) .
C0007634
cells
C0699040
of cell surface