thus , whereas 2-day cultured rinm5f cells from the eighth tumour passage exhibited a greater than 100-fold increment of insulin content and release , with enhanced secretory responsiveness to leucine , arginine , theophylline , k+ ( 25 mmol/l ) or ca2+ ( 7.6 mmol/l ) , rinm5f cells cultured for a further 19 days had almost completely lost the attributes resulting from 259 days of maintenance in vivo .
C1327616
secretory
C0007634
cells