treatment of i-cell cultures with cycloheximide or tunicamycin demonstrated that ( 1 ) i-cell fibroblasts rapidly excrete all newly synthesized beta-hexosaminidase , ( 2 ) two qualitatively distinct pools of beta-hexosaminidase isoenzymes exist inside i-cell fibroblasts , one of which is a rapid-turnover excretory pool , and ( 3 ) the induction of an abnormal glycosylation of beta-hexosaminidase by tunicamycin in normal or i-cell fibroblast cultures does not affect subsequent excretion of the enzyme .
C0022173
isoenzymes
C0014442
enzyme