by combining anion exchange chromatography , gel filtration , agarose gel electrophoresis , hydrophobic interaction chromatography , and affinity chromatography on iminodiacetic acid-sepharose cl 4b and lysine sepharose 4b we were able to isolate seven different water soluble reiter antigens from one single reiter sonicate supernatant ( i , ii , iii , iv , v , vi ) .
C0003075
anion
C0021966
i