after hypotonic treatment of the colony cells and their attachment to the slides , the cells were fixed by a three-step procedure as follows : addition of a 30 % fixative ( 3:1 methanol : acetic acid ) diluted with the hypotonic solution , addition of 20 % ethanol , and subsequent immersion of the slides in a 100 % fixative .
C0001963
methanol
C0001962
ethanol