when 13c nmr spectra were obtained of the acetylated protein bound to multilamellar vesicles prepared from dimyristoylphosphatidic acid in the gel state ( t = 33 degrees c ) , conditions under which reduced motion in the lipid bilayer broadens methylene and methyl 13c resonances of the membrane beyond detection ( i.e . greater than 75-100 hz ) , line widths of membrane-bound protein were measured to be 7.8 hz , an increase of 4 hz versus free protein .
C0025252
membrane
C0033684
protein