this conclusion derives from the following three facts : ( 1 ) the two enzyme activities can not be separated upon velocity sedimentation in 1.7 m urea , ( 2 ) both activities elute at a pi of 5.25 upon chromatofocussing and ( 3 ) after sds-electrophoresis , renaturation of the enzymes in situ and measurement of dna polymerase and primase activities in the gels , both enzymes have identical mobilities and coincide with the high mol . wt . catalytic subunit of dna polymerase alpha .
C0012892
dna polymerase
C0012888
dna polymerase alpha