in the standard [ 3h ] ouabain-binding assay for quantification of the na , k-atpase ( na+ + k+-dependent atpase ) concentration in rat skeletal muscles , samples are incubated for 2 x 60 min in 1 microm- [ 3h ] ouabain at 37 degrees c followed by a wash-out for 4 x 30 min at 0 degree c .
C0001473
atpase
C0001479
na