31p nmr measurements were made ( at 121.5 mhz and 5 degrees c ) on enzyme-bound substrate complexes of 3-phosphoglycerate kinase in order to address three questions pertaining to ( i ) the integrity of the enzyme-substrate complexes with mg ( ii ) in the presence of sulfate concentrations typical of those used for crystallization in x-ray studies , ( ii ) the relative affinities of mg ( ii ) to atp bound at the two sites on the enzyme , and ( iii ) the ph behavior of the different phosphate groups in the enzyme complexes .
C0014442
enzyme
C0026741
enzyme complexes