following previous findings that feeding a choline-deficient ( cd ) diet to rat strongly promotes the evolution of liver cells , initiated by a chemical carcinogen , to foci of gamma-glutamyltranspeptidase ( gamma gt ) -positive hepatocytes , we investigated whether a cd diet could also promote the evolution of gamma gt-positive foci to hepatomas . gamma gt-positive foci were induced in male sprague-dawley rats by administration of a single dose of diethylnitrosamine followed by 2 weeks ' feeding on a cd diet containing 0.02 % acetylaminofluorene .
C0227525
hepatocytes
C2239176
hepatomas