livers of rats fasted for 18 hr were perfused for 60 min at 37 , 35 , and 32 degrees c in a simple noncirculating system with a carbogen ( 95 % , 5 % co2 ) equilibrated , oxygen carrier-free , glucose-containing ( 200 mg x dl-1 ) cristalloid perfusate ( ph 7.4 , 280 mosmol x kg-1 ) without or with the addition of insulin ( 10 u x hr-1 ) , and analyzed for tissue atp , adp , amp , acetoacetate , and beta-hydroxybutyrate as well as for parameters of the energy metabolism of isolated mitochondria .
C0021641
insulin
C0014272
energy metabolism