in the first study , we microinjected fluorescently labeled tubulin into mitotic ptk1 cells and measured fluorescence redistribution after photobleaching ( frap ) to determine the assembly dynamics of the microtubules within the chromosomal fibers in metaphase cells depleted of nonkinetochore microtubules by cooling to 23-24 degrees c .
C0041348
tubulin
C0026046
microtubules