the procedures involved pulsing cells at 15 degrees c with low density lipoprotein that had been reconstituted with [ 3h ] cholesteryl oleate and then incubating the cells at 37 degrees c in the presence of a sterol acceptor , while monitoring both the hydrolysis of [ 3h ] cholesteryl oleate in lysosomes and the efflux of the resulting [ 3h ] free cholesterol to the acceptor .
C0007634
the
C0024369
lysosomes