to study the distribution and the degree of polymerization of actin in regenerating and neoplastic cells we have examined : ( 1 ) the immunofluorescent staining of these cells with human antiactin antibodies ( aaa ) , ( 2 ) the sensitivity of cellular actin to aaa staining after treatment with the actin-depolymerizing factor present in plasma or serum of several animal species , and ( 3 ) the total and relative amounts of f- and g-actin in tissue preparations , determined by means of differential centrifugation and densitometric analysis of stained sodium dodecyl sulfate-polyacrylamide gels .
C0007634
cellular
C0016890
g-actin