however , with very water-insoluble agents , edta pretreatment usually gave a significant , 2-5-fold increase in mutation , viz . with 2nf , bp and cap ( trp locus ) but not with 9aa . 2ap , which was moderately soluble in distilled water , only showed a small significant increase at the a2c locus after edta pretreatment .
C0043047
water
C0790233
distilled water