in the preceding paper ( shechter , y. , and ron , a . ( 1986 ) j. biol . chem . 261 , 14945-14950 ) we have shown that in fat cells , prepared and maintained in an isotonic buffer ( ph 7.4 ) containing neither phosphate nor bicarbonate anions ( buffer a ) , the dose-response curve to insulin shifted to the right by about 2 logarithms and insulin binding affinity or capacity was only slightly decreased .
C0031603
phosphate
C0003075
anions