while normal glutaraldehyde , formaldehyde and osmium tetroxide treatment failed to permeate and preserve intracellular structures , several cycles of rapid freezing ( - 155 degrees c ) and thawing followed by glutaraldehyde fixation and osmium tetroxide post-fixation demonstrated the intracellular details of the majority of cells so treated .
C0729605
intracellular structures
C0007634
the