an extracellular zn-endopeptidase was purified to homogeneity from the culture filtrates of streptococcus faecalis ( human oral strain 0g1-10 ) by a procedure that comprised concentration in an amicon hollow fiber system , ammonium sulfate precipitation , gel permeation chromatography , hydrophobic interaction chromatography ( batch operation on phenyl-sepharose cl-4b ) , followed by fast protein liquid chromatography ( fplc ) on a phenyl-superose hr 5/5 column , and finally fplc on a superose 12 hr 10/30 column .
C0184661
procedure
C0543467
operation