the straws were thawed by 1 of 3 methods : ( 1 ) room temperature for 10 minutes and 37 degree c hot plate for 10 minutes , ( 2 ) ice water for 10 minutes and 37 degree c hot plate for 10 minutes , ( 3 ) 35 degree c water for 12 seconds and 37 degree c hot plate for 10 minutes .
C0020746
ice water
C0043047
water