when some autolytic digestion was permitted by extracting lysosomal fractions in an acidic medium ( 0.2 % triton x-100 , 0.1 m sodium acetate buffer , ph 5.2 ) at 0-4degrees c and omitting p-nitrophenyloxamic acid from the gradient , the acidic form of beta-glucuronidase and the intermediate form of acid phosphatase were lost , the isoelectric points of the acidic forms of acid phosphatase , arylsulfatase and beta-n-acetylhexosaminidase were increased 0.6-1.2 units , and the isoelectric point of the basic forms of acid phosphatase , arylsulfatase and beta-glucuronidase was increased 0.5 unit .
C0031678
phosphatase
C0001109
acid phosphatase