these observations constitute an efficient approach to the synthesis of larger polynucleotides from a specific series of oligonucleotide blocks since ( i ) , the methoxyethyl group can be easily introduced into each oligonucleotide using the single addition reaction catalyzed by polynucleotide phosphorylase in the presence of a 2'-0- ( alpha-methoxyethyl ) nucleoside 5'-diphosphate , and ( ii ) , the blocking group may be readily removed under mild conditions after each successive ligation reaction .
C0032550
polynucleotides
C0028953
oligonucleotide