at ph 7.0 and 25 degrees c , nbf-cl ( 4-chloro-7-nitrobenzofurazan ) reacts rapidly with rabbit muscle aldolase ( d-fructose-1,6-bisphosphate d-glyceraldehyde-3-phosphate-lyase , ec 4.1.2.13 ) to yield a product with an absorption maximum at 402 nm , which is shifted to 422 nm upon acid denaturation .
C0016762
aldolase
C0024188
lyase