studies of the initial rates of the cbh-catalyzed production of cellobiose from fibrous alpha-cellulose show that ( i ) the dissociation constant for cellobiose competitive product inhibition of the reaction is ki = ( 1.13 +/- 0.37 ) x 10 ( -3 ) m , ( ii ) the adsorption of cbh on fibrous alpha-cellulose and its subsequent reaction conform to kinetic equations developed in conjunction with the langmuir adsorption isotherm , ( iii ) the rate-ph curve has a maximum at ph 5.2 and decreases at higher and lower ph values , exhibiting enzyme pk values of 3.8 and 6.5 , and ( iv ) the energy of activation of the overall reaction between 5 and 60 degrees c is 5.3 +/- 0.3 kcal mol-1 at ph 5.2 .
C0007630
cellobiose
C0007648
alpha-cellulose