a phospholipase a2 bound tightly to the particulate fractions of rat ascites hepatoma cells was purified approximately 13,000-fold with a reasonably high yield ( 34 % ) by extraction with sodium cholate , ammonium sulfate fractionation , solubilization with sodium dodecyl sulfate , column chromatographies on sephadex g-150 in the presence of sodium dodecyl sulfate , and on deae-cellulose and cm-cellulose in the presence of triton x-100 .
C0011050
deae-cellulose
C0007648
cellulose