microtubular protein extracted with charcoal in a buffer containing 1 mm edta showed an absolute dependence on gtp for assembly , whereas , microtubular protein extracted with charcoal in a buffer containing 1 mm mgcl2 could assemble with the nucleotide analogs , guanosine-5'- ( beta , gamma-imido ) triphosphate ( gmp-pnp ) and guanosine-5'- ( beta-gamma-methylene ) triphosphate .
C0018353
gtp
C0018366
gmp-pnp