recovery of primary immune complexes with use of the bacterial immunoadsorbant ( a ) allowed second-antibody incubation time to be shortened from 3 h to 20 min at 37 degrees c , ( b ) decreased by 80 % the amount of second antibody required per assay , thereby significantly reducing assay costs , and ( c ) provided a convenient and reproducible method for separation of free and bound antigen .
C0003313
immune complexes
C0003241
antibody