polysomes were isolated from the mitochondria/endoplasmic reticulum complex ( fraction c ) by treatment with 2 % triton x-100 and 0.5 % sodium deoxycholate in the presence of 50 mm tris-hcl , ph 7.6 , 0.1 m kcl , 0.15 m nh4cl and 50 mm mg ( ch3coo ) 2 and purified through sucrose layers of decreasing ionic strength containing 2 mg/ml of cell sap proteins .
C0014239
endoplasmic reticulum
C0007634
cell