after initial screening to identify antigen-binding monoclonal antibodies , one or more of the clones are isolated by limiting dilution cloning , grown in ascites , and the resulting antibodies secreted into the ascitic fluid are affinity purified on sepharose-bound protein a , radiolabeled , and cross-compared with antibodies from other clones by a solid-phase competitive immunoassay .
C1148575
antigen-binding
C0003241
antibodies