incubation of dna under conditions leading to renaturation of its single-stranded fraction led to an increase in transforming activity for the pura16+ marker ( close to the origin of replication ) relative to leu-8+ and metc3+ markers ( located in the middle of the chromosome ) , indicating this region is the main source of the single-stranded fraction .
C0242961
origin of replication
C0008633
chromosome