biotin-deficient cells were depleted of all amino acids at 26 and 43 hr , with alanine , arginine , aspartate , cysteine , glutamate , isoleucine , leucine , lysine , methionine , serine , threonine , and valine being present in less than half the concentration observed in biotin-optimal cells .
C0002520
amino acids
C0001898
alanine