the growth of gh4c1 , gh3 , gh1 , and gh3c15 rat pituitary tumor cell lines was studied in a serum-free medium ( designated trm-1 ) formulated with 1:1 ( vol/vol ) mixture of ham 's f12 nutrient mixture and dulbecco 's modified eagle 's medium ( f12-dme ) containing 15 mm 4- ( 2-hydroxyethyl ) -1-piperazineethanesulfonic acid ( hepes ) , 50 micrograms/ml gentamicin supplemented with 10 micrograms/ml bovine insulin , 10 micrograms/ml human transferrin ( tf ) , 10 ng/ml selenous acid , 10 nm 3,5,3'-triiodothyronine ( t3 ) , 50 microm ethanolamine ( etn ) , and 500 micrograms/ml bovine serum albumin .
C0018270
growth
C1333673
gh1