theca cells from large porcine follicles ( 8-10 mm ) were plated ( 5 x 10 ( 5 ) cells/ml.well ) in serum-free m199 , treated with increasing doses of growth factors : igf-1 ( 0.1-50 ng/ml ) , igf-ii ( 0.5-200 ng/ml ) , egf ( 0.021-100 ng/ml ) , tgf beta ( 0.001-40 ng/ml ) , or insulin ( 0.01-50 micrograms/ml ) , with or without human cg [ ( hcg ) ; 20 ng/ml ] , and incubated for 72 h .
C0242275
egf
C0086418
human