the recombinant ( r- ) pepsinogen , which was insoluble , was recovered by centrifugation , washed , dissolved in 6 m urea in tris-hcl , ph 8 , and refolded by rapid dilution . r-pepsinogen was purified to homogeneity after chromatography on sephacryl s-300 and fast protein liquid chromatography on a monoq column . r-pepsinogen contains an additional methionine residue at the nh2 terminus as compared to native ( n- ) pepsinogen .
C0077923
a
C0030913
pepsinogen