a kinetic scheme for the overall catalytic mechanism is proposed , which is characterized by ( 1 ) formation of a michaelis complex between enzyme and substrate , ( 2 ) the reductive step involving partly rate-limiting scission of the substrate c-h bond , ( 3 ) reaction of the complex of reduced enzyme and aldehyde with dioxygen , and ( 4 ) a significant contribution of the dissociation rate of product from its complex with reoxidized enzyme to the overall rate .
C0014442
enzyme
C0026741
complex