biochemical analysis shows that this mutant , which requires glutamate for growth , releases fixed n2 as nh4+ into the environment because of ( i ) constitutive synthesis of nitrogenase and ( ii ) genetic blocks resulting in losses of glutamate synthase [ l-glutamine:2-oxoglutarate aminotransferase ( nadph oxidizing ) , ec 2.6.1.53 ] and glutamate dehydrogenase [ l-glutamate : nadp oxidoreductase ( deaminating ) , ec 1.4.1.4 ] activities , enzymes essential for nh4+ assimilation into cell material .
C0028174
nitrogenase
C0030016
ec