the endogenous calmodulin-dependent kinase in cold-stable microtubule fractions was identical to a previously purified calmodulin-dependent kinase from rat brain by several criteria including ( 1 ) subunit molecular weights , ( 2 ) subunit isoelectric points , ( 3 ) calmodulin-binding properties , ( 4 ) subunit autophosphorylation , ( 5 ) calmodulin-binding subunit composition on high-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis , ( 6 ) isolation of kinase on calmodulin affinity resin , ( 7 ) kinetic parameters , ( 8 ) phosphoamino acid phosphorylation sites on beta-tubulin , and ( 9 ) phosphopeptide mapping .
C0026046
microtubule
C0005290
beta-tubulin