the 110k-cm was purified using atp extraction of brush border cytoskeletons in the presence of an atp regenerating system , gel filtration of the solubilized extract , an atp depletion step to specifically precipitate 110k-cm with f-actin , and resolubilization followed by phosphocellulose chromatography .
C0010853
cytoskeletons
C1180307
f-actin