when hypothalamic slices from five animals were perfused in a chamber with artificial cerebrospinal fluid ( acsf ) at a flow rate of 100 microliters/min under a gaseous phase of 95 % o2 and 5 % co2 at 37 c , rat ( r ) grf- and srif-like immunoreactivities ( -li ) were constantly detected in 30-min perifusates at least until 240 min of perifusion , and during the perifusion with 60 mm k+ , the concentrations of rgrf-li and srif-li were increased 2.1 and 3.2 times , respectively , over basal values .
C0020663
hypothalamic
C0023610
grf