cells similarly heated in magnesium-conserving buffers [ ph 7.2 , 50 mm tris ( hydroxymethyl ) aminomethane containing 10 mm mgcl2 or piperazine buffer ] did not leak rna , suffered no ribosomal damage when heated for 15 min , and recovered , at least partially , in the presence of actinomycin d .
C0024467
magnesium
C0024472
mgcl2