hybridoma cells , derived from sp 2/0 myeloma and secreting a mab to a human milk fat globule membrane-associated high molecular weight glycoprotein , were cloned in this medium containing 1 % ( v/v ) fetal calf serum and then sequentially adapted in serum-free medium further supplemented with transferrin and insulin , both at 10 micrograms/ml .
C0007634
cells
C1327616
secreting