collagenase-isolated , ficoll-purified pancreatic islets ( median 21,000 , 15 % of total islet yield ) from eight cadaver pancreases were cultured in vitro for 24 h , equilibrated in three steps with dimethyl sulfoxide ( dmso ) to a 2-m concentration , supercooled , nucleated , and cooled at 0.25 degree c/min to -40 degrees c before storage at -196 degrees c for 44.25 +/- 8.75 days .
C0022131
pancreatic islets
C0030274
pancreases