a lysophospholipase from escherichia coli cells was purified about 1,500-fold to near homogeneity by extraction with tris-hcl buffer , streptomycin treatment , ( nh4 ) 2so4 fractionation , column chromatographies on sephadex g-200 , deae-cellulose and hydroxylapatite-cellulose , and polyacrylamide gel electrophoresis .
C0011050
deae-cellulose
C0007648
cellulose