to examine their possible roles in the transcription and translation of aav mrna , 293-31 cells , a human embryonic kidney cell line that constitutively expresses the ad early region ia and ib gene products , were transfected with a pbr325 plasmid ( plh1 ) that contains a duplex aav2 dna segment ( 0.03-0.97 map units ) which encompasses the promoters and coding sequences necessary for expression of all aav polypeptides .
C0007634
cells
C0015295
coding sequences