from our data we conclude that ( 1 ) t4 endonuclease v binds to uv-irradiated dna but not to dna that has been previously incised by the endonuclease , ( 2 ) equilibrium between the free and complexed form of the enzyme is attained under our reaction conditions , ( 3 ) dissociation of enzyme -- dna complexes is retarded by sodium cyanide , and ( 4 ) retention of enzyme -- dna complexes on nitrocellulose filters is enhanced by high concentrations of saline -- citrate .
C0026741
complexed
C0014442
enzyme