although there was no detectable expression from the 5b open reading frame when it was preceded by the native promoter and ribosome binding site or by a tac promoter and the native ribosome binding site , we have overproduced a 5b protein both in vitro and in escherichia coli cells by using a tac promoter and a specially-designed synthetic ribosome binding site . beta-galactosidase fusion studies suggested that the synthetic binding site is at least 150-fold more efficient than the native binding site .
C0079941
open reading frame
C0007634
cells