individual human neutrophils ( pmn ) isolated by hypaque-ficoll gradient sedimentation , dextran sedimentation , or buffy coat preparation were assessed for the effects of leukotriene b4 ( 5s,12r dihydroxy 6,14-cis-8 , 10 trans eicosatetraenoic acid ( ltb4 ) -pretreatment on n-formylmethionyl-leucyl-phenylalanine ( fmlp ) -mediated membrane potential or oxidative responses by using flow cytometry and a lipophilic probe of membrane potential ( di-pentyl-oxacarbocyanine , di-o-c ( 5 ) 3 ) , or the nitroblue tetrazolium dye ( nbt ) reduction test , respectively .
C0086418
human
C3538902
di