conditions for clonal growth of secondary cultures of bronchial epithelial cells were optimized by coculturing the human cells with mitomycin c growth-arrested swiss 3t3 mouse feeder cells , lowering the calcium concentration of medium m199 , and supplementing it with hydrocortisone , insulin , cholera toxin , epidermal growth factor , and 1.25 % fetal bovine serum .
C0086418
human
C0242275
epidermal growth