fowl anterior pituitary glands were bisected and each half was pretreated in either medium 199 or medium containing egta to deplete endogenous calcium ( ca2+ ) stores , after which they were incubated in medium 199 , or ca2+-free medium , containing prolactin release-stimulating agents and verapamil , a ca2+ channel blocker .
C0032008
anterior pituitary glands
C0033371
prolactin