( upon prolonged incubation in the buffer , however , tubulin undergoes irreversible changes according to apparent first-order kinetics with a half-life of approximately 8 h. these changes include the irreversible release of nucleotide , a loss of the ability to polymerize , and a decrease in molar ellipticity between 210 and 240 nm . )
C0022702
kinetics
C0018517
half-life