this was done by enzymatic manipulations in vitro of the yeast trnaasp , involving specific hydrolysis with s1-nuclease and rnaase a , phosphorylation with t4-polynucleotide kinase and ligation with t4-rna ligase : it allowed us to replace the normal anticodon guc by trinucleotides axy and to introduce simultaneously a 32p-labelled phosphate group between the uridine at position 33 and the newly inserted adenosine at position 34 .
C0020291
hydrolysis
C0014241
rnaase