specific staining of sialic acid components after sodium dodecyl sulfate polyacrylamide gel electrophoresis can be carried out as follows : 1 ) extract glycoprotein of erythrocyte membranes or serum by the phenol-saline method , 2 ) electrophorese the extract on 5 % polyacrylamide gel containing 0.1 % sodium dodecyl sulfate at constant current , 3 ) treat the gel with chilled 0.04 m hio4 for 45 minutes , 4 ) replace the periodic acid solution with one containing resorcinol 0.6 g , conc .
C0031428
phenol
C0035197
resorcinol