the approach is demonstrated using the titration ( that is , the determination of the molar concentration of the enzyme active centers ) of purified cellobiohydrolase i ( cbh i ) ( ec 3.2.1.91 ) of the fungus trichoderma reesei . p-nitrophenyl-beta-d-lactoside was used as a reference substrate ( km = 0.5 mm ) , and cellobiose and cm-cellulose as specific ligands .
C0007630
cellobiose
C0007648
cellulose