in this assay , cells are incubated with [ 3h ] dexamethasone at 4 c or 21 c , treated for 15 min at 4 c with the sulfhydryl blocking reagent sodium tetrathionate or methyl methanethiolsulfonate , and then ruptured with the neutral detergent triton x-100 to separate the nuclei from the cytoplasmic constituents .
C0007634
cells
C0010834
cytoplasmic