heptaminol and its internal standard n-propylamine were salted out from plasma together with acetonitrile , the previously proposed `` '' solvent demixing `` '' extraction procedure . both amines were derivatised in acetonitrile with the o-phthaldialdehyde , 2-mercaptoethanol procedure of roth . the adducts were quantitated by reversed-phase high-performance liquid chromatography on radial-pak cartridges with ultraviolet detection . peak height ratios were linearly related to concentrations up to 250 mumol l-1 with a 2 % coefficient of variation . sensitivity was 3.5 mumol l-1 ( signal-to-noise ratio of 5 ) . means of the usual pharmacokinetic parameters in four dogs were : elimination half-life 3.75 h , apparent distribution volume 2.18 l kg-1 and total clearance 0.402 l kg-1 h-1 , similar to the results obtained in humans by other authors using radiolabelled heptaminol .
C0033504
propylamine
C0002508
h amin