the opaque material could be interpreted as the result of direct interaction between mitochondria and the laser beam , even though the mitochondria were noted outside of the previously estimated focal spot size of about 3 micro within the thickness of 2-4 micro of monolayered cells , larger areas of damage can be accounted for by divergence of the beam which is focused by a microscope objective of very short focal length .
C0026237
mitochondria
C0007634
cells