changes of free cytosolic adp concentrations , needed for the calculation of dg , were determined by two indirect methods since a direct measurement is not feasible : ( i ) via the mass action ratio of the creatine kinase reaction ( ck ) assuming near equilibrium conditions , and ( ii ) via quantification of the net hydrolysis of atp to adp by a detailed balancing of possible contribution to pi production .
C0010287
creatine kinase
C1510699
hydrolysis