specificity of griffonia simplicifolia i reaction with carbohydrate determinants at the cell surface is shown by ( i ) ability of alpha-galactosidase treatment of intact cells to abolish all lectin binding whereas beta-galactosidase has no effect on lectin binding , ( ii ) ability of methyl alpha-d-galactopyranoside to completely inhibit lectin binding with methyl alpha-d-galactopyranoside having no effect on lectin binding , ( iii ) ability of brief treatment of intact cells with trypsin to liberate a glycopeptide but reacts with g. simplicifolia i to form a precipitate that is dissolved by addition of methyl-alpha-d-galactopyranoside or alpha-galactosidase , ( iv ) ability of methyl alpha-d-galactopyranoside ( but no other monosaccharide ) to completely inhibit avid binding of macrophages to g. simplicifolia i lectin immobilized on an insoluble support , and ( v ) ability of immobilized lectin to separate macrophages into highly pure subpopulations of lectin-reactive and lectin-unreactive cells , as shown by examination of fluorescein-labeled lectin-treated cells with phase-contrast/fluorescence microscopy .
C0007004
carbohydrate
C0026492
monosaccharide