to determine whether the akr-mca cells growth arrest at a unique point in g1 or whether they arrest in a physiologic state which can also be achieved by the nontransformed cells , akr-2b cells were maintained in medium with 10 % serum containing the mitogens , epidermal growth factor ( egf ) or 12-o-tetradecanoylphorbol-13-acetate ( tpa ) , until they reached saturation density or were arrested at subconfluence by artificial deletion of amino acids from the medium .
C0007595
the
C0007634
cells