for assay of epf , 0.25 ml of 1:2 step diluted anti-human lymphocyte serum ( als ) , 0.05ml of guinea pig serum as complement and 0.1ml of lymphocytes suspension ( 1 x 10 ( 7 ) /ml ) pretreated with test serum were mixed and then incubated at 37 degrees c for 90 min .
C0229671
serum
C0009498
complement