such studies require : use of appropriate controls for splenectomy and young red cell age , tracing a defect through affected family members , verifying that a defect corresponds to the appropriate heredity pattern , for example that a heterozygote for an autosomal dominant defect had 50 % abnormal protein , differentiating the effects of splenic or circulatory conditioning from the primary red cell defects , verifying that the defect is present in the intact cell and is not secondary to experimental manipulations , distinguishing an unrelated , linked polymorphism from the primary mutation responsible for the disorder .
C0037995
splenectomy
C0037993
splenic