# Proteomics/Protein Sample Preparation

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## Introduction

As technological advances are made in the field of proteomics, it is
seen that advances are necessary in the preparation of protein samples
prior to any particular procedure. A number of issues arise in this
respect; including sample clean-up, fractionation, enrichment, and the
also sample condition optimization. Considerations of this nature can be
crucial in obtaining relevant results from an experiment; some so much
so that experts feel the field of proteomics is currently being limited
by the lack of significant advancement in sample preparation techniques.

This facet of proteomics is becoming particularly critical in the case
of high throughput protocols where the necessary conditions of a sample
in one stage may directly conflict with the efficacy of a second stage.
For example, during the initial step in 2D electrophoresis, isoelectric
focusing, all proteins in a sample are given a net charge of zero; while
the second step, gel electrophoresis, requires a negative charge on all
products in the sample in order to induce movement through the gel
matrix.

Many companies offer pre-packaged kits that will allow you to prepare
samples for many different techniques. They also offer many protein
samples, and other protein technologies. Many of these companies are
also on the forefront of protein analysis technology. Some examples are:

1.  www.millipore.com
2.  www.bio-rad.com
3.  www.qiagen.com
4.  www.gelifesciences.com
5.  www.invitrogen.com
6.  www.eksigent.com
7.  www.agilent.com
8.  www.beckmancoulter.com
9.  www.caliperls.com
10. www.glygen.com
11. www.piercenet.com
12. www.bioproximity.com

This section is part of an ongoing project at the Rochester Institute
of Technology, involving the
Bioinformatics department. Currently
the project is being worked on by a Proteomics Class taught by Dr. Paul
Craig.

## Bibliography

-   Jörg von Hagen, VCH-Wiley 2008 Proteomics Sample Preparation.
    
