Ho hlahlojoa ha liprotheine ka tsela ea nuclear magnetic resonance spectroscopy ke eng?
Nuclear magnetic resonance spectroscopy ea liprotheine (eo hangata e khutsufalitsoeng e le protheine NMR) ke tšimo ea baeloji ea sebopeho eo ho eona NMR spectroscopy e sebelisetsoang ho fumana leseli mabapi le sebopeho le matla a liprotheine, hammoho le li-acid tsa nucleic, le likarolo tsa tsona. Lefapha lena le ile la qaptjoa ke Richard R. Ernst le Kurt Wüthrich ETH, le Ad Bax, Marius Clore, Angela Gronenborn NIH, le Gerhard Wagner Univesithing ea Harvard, har'a ba bang. Ho khetholla sebopeho ka NMR spectroscopy hangata ho na le mekhahlelo e'maloa, e' ngoe le e 'ngoe e sebelisa mekhoa e fapaneng e ikhethang haholo. Ho lokisetsoa sampole, ho etsoa litekanyo, ho sebelisoa mekhoa ea ho hlalosa,'me ho baloa le ho tiisoa ha sebopeho.  NMR e akarelletsa litšobotsi tsa quantum-mechanical tsa motheo o bohareng ("nucleus") oa athomo. Litšobotsi tsena li itšetlehile ka tikoloho ea moo limolek'hule li leng teng,'me ho lekanya ha tsona ho fana ka'mapa oa kamoo liathomo li hokahaneng ka lik'hemik'hale kateng, hore na li haufi hakae sebakeng, le hore na li tsamaea ka lebelo le lekae ha li bapisoa. Lintho tsena li tšoana hantle le tse sebelisoang ha ho hlahisoa litšoantšo tse tsebahalang haholo tsa matla a khoheli (MRI), empa likopo tsa limolek'hule li sebelisa mokhoa o fapaneng hanyane, o loketseng phetoho ea sekala ho tloha ho limilimithara (tse khahlisang litsebi tsa radiology) ho isa ho li-nanometer (liathomo tse kopantsoeng hangata li arohane ka karoloana ea nanometer), e leng karolo ea milione. Phetoho ena ea sekala e hloka kutloisiso e phahameng haholo ea ho lemoha le botsitso bakeng sa litekanyo tsa nako e telele. Ho fapana le MRI, lithuto tsa baeloji ea sebopeho ha li hlahise setšoantšo ka ho toba, empa li itšetleha ka lipalo tse rarahaneng tsa k'homphieutha ho hlahisa mefuta ea limolek'hule tse tharo.  Hona joale mehlala e mengata e hlahlojoa ka tharollo ea metsi, empa mekhoa e ntse e hlahisoa hore e sebetse le mehlala e tiileng. Ho bokella boitsebiso ho itšetlehile ka ho beha sampole ka har'a makenete a matla, ho romela matšoao a maqhubu a seea-le-moea ka sampole, le ho lekanya ho monngoa ha matšoao ao. Ho itšetlehile ka tikoloho ea liathomo ka har'a protheine, liathomo tsa liathomo ka bomong li tla monya maqhubu a fapaneng a matšoao a seea-le-moea. Ho feta moo, lipontšo tsa ho monya tsa li-nucleus tse fapaneng li ka 'na tsa sitisoa ke li-nucleus tse haufi. Boitsebiso bona bo ka sebelisoa ho lekanya sebaka se lipakeng tsa li - nucleus. Maemo ana a ka boela a sebelisoa ho fumana hore na protheine e entsoe joang.  Phuputso e tloaelehileng e ka 'na ea akarelletsa kamoo liprotheine tse peli li sebelisanang kateng, mohlomong ka sepheo sa ho hlahisa limolek'hule tse nyenyane tse ka sebelisoang ho hlahloba tlhaho e tloaelehileng ea ho sebelisana ("bioloji ea lik'hemik'hale") kapa ho fana ka tataiso e ka khonehang bakeng sa tšebeliso ea meriana (tlhahiso ea lithethefatsi). Hangata, liprotheine tse sebelisanang li ka 'na tsa fumanoa lithutong tsa liphatsa tsa lefutso tsa batho, tse bontšang hore tšebelisano e ka sitisoa ke liphetoho tse sa thabiseng, kapa li ka 'na tsa phetha karolo ea bohlokoa bophelong bo tloaelehileng ba "mohlala" oa lintho tse phelang tse kang ntsintsi ea litholoana, tomoso, sebōkō C. elegans, kapa litoeba. Ho lokisetsa sampole, mekhoa ea limolek'hule tsa likokoana-hloko e atisa ho sebelisoa ho etsa bongata ka ho belisoa ke baktheria. Sena se boetse se lumella hore ho fetoloe sebopeho sa isotope ea molek'hule, e leng se lakatsehang hobane li - isotope li itšoara ka tsela e fapaneng'me li fana ka mekhoa ea ho khetholla matšoao a NMR a koahelang.