Iyini i- GeneBass?
I-GeneBass iyipulatifomu yokuhlola i-CRISPR-Cas9 yokuhlaziywa kokusebenza okuphezulu kwamagciwane kumaseli ezilwane ezincelisayo. Isikhulumi seGeneBass sivumela abacwaningi ukuba basebenzise noma bacindezele ngokuhlelekile izakhi zofuzo ezithakazelisayo futhi batadishe imiphumela yazo ezinqubweni zamaseli njengokwanda kwamaseli, ukuhlukaniswa, nokusinda. Lolu hlelo lusebenzisa umtapo wezincwadi we-lentiviral of guide RNAs okubhekiswe kuwo wonke ama-gene e-genome yomuntu noma yegundane, angangeniswa kumaseli ngokusebenzisa i-transduction yegciwane. Ama-RNA aqondisayo aklanyelwe ukuqondisa izifunda ezithile ze-genome eziseduze nezindawo zokuqala zokubhalwa kwezakhi zofuzo ngazinye, okuvumela ukuvuselelwa noma ukucindezelwa kwezakhi zofuzo okuqondile futhi okusebenzayo. Ukuhlolwa kweGeneBass ngokuvamile kuhilela ukuguqulwa kwenani elikhulu lamaseli, kulandelwe ukukhethwa noma ukuhlunga kwamaseli ngokususelwa kuma-phenotypes athile anentshisekelo. Ukulandelana kwezakhi zofuzo ezihamba phambili kwe-RNA ngaphambi nangemva kokukhethwa kungasetshenziselwa ukukhomba izakhi zofuzo ezibalulekile ezinqubweni ezithile zamaseli noma ezihilelekile ezindleleni zezifo.