oxford nanopore technologies limited is a u.k.-based company which is developing and selling nanopore sequencing products ( including the portable dna sequencer , minion ) for the direct , electronic analysis of single molecules .

oxford nanopore technologies

oxford nanopore technologies <EOT> oxford nanopore’s minion™ usb - attached miniature sensing device . photo : oxford nanopore technologies

press release : oxford nanopore technologies : new study : oxford nanopore technologies ltd . - strategic swot analysis review

press release : oxford nanopore technologies : new report available : oxford nanopore technologies ltd . - medical equipment - deals and alliances profile

the minion™ dna sequencer from oxford nanopore technologies fits in the palm of a hand . ( photo credit : oxford nanopore technologies )

( pr - inside . com ) oxford nanopore technologies ltd . ( oxford nanopore ) , formerly oxford nanolabs ltd . , i . . .

james brayer , oxford nanopore technologies inc . , cambridge , ma , united states

oxford nanopore : oxford nanopore ' s valuation hits £1 . 5bn after asia - pac funding round

oxford nanopore technologies develops nanopore - based electronic systems for analyzing single molecules , including dna , rna and proteins .

oxford nanopore technologies a uk company that offers several portable realtime dnarna sequencers com . . .

37 . © copyright 2016 oxford nanopore technologies ⎪ 37 concurrent reads migration to monogodb3 . 0

oxford nanopore technologies , an affordable dna sequencing devices supplier seeking to break the stra . . .

oxford nanopore technologies wants to get your dna online , and that future is closer than you think .

oxford nanopore technologies has tapped a global syndicate of investors for $ 140 million to fuel its . . .

real - time dna sequencer firm oxford nanopore technologies has raised £100m to support its next phase . . .

technology focus from clive g brown , cto of oxford nanopore technologies . the minion is for research use only .

a tiny new device called the minion , developed by oxford nanopore technologies , promises to help sci . . .

oxford nanopore technologies ltd is developing a new generation of nanopore - based electronic systems for analysis of single molecules , including dna , rna and proteins .

oxford nanopore technologies , facing a lawsuit from illumina , revealed that this microscopic pore , csgg , underlies its new devices .

oxford nanopore ' s ceo , gordon sanghera , currently has an approval rating of 100 % . oxford nanopore ' s primary competitors are

oxford nanopore develops and commercializes nanopore - based dna , rna and protein molecule sensing system . oxford nanopore ' s headquarters is located in oxford science park , england . for every employee , oxford nanopore generates $ 36 . 7k in revenue . oxford nanopore ' s latest funding round was a equity for $ 140m on mar 2018 . oxford nanopore ' s top competitor is pacbio , led by michael hunkapiller , who is their chairman & ceo . oxford nanopore has 205 followers on owler .

oxford nanopore develops and commercializes nanopore - based dna , rna and protein molecule sensing system . oxford nanopore was founded in 2005 . oxford nanopore ' s headquarters is located in oxford science park , england , gb ox4 4dq . it has raised 658 . 4m i . . .

oxford nanopore technologies . pcr - based , pcr - free , and rapid barcoding for minion and promethion libraries . available at : urltoken .

oxford nanopore technologies . voltrax : a rapid , programmable , portable , disposable device for sample and library preparation . available at : urltoken .

owler has collected 12 screenshots of oxford nanopore ' s website since aug 2014 . the latest oxford nanopore website design screenshot was captured in oct 2017 .

oxford nanopore technologies ltd . develops technologies for molecular detection and analysis in the united kingdom . the company offers base technology , a system for dna sequencing that employs nanopores to process , identify , and record dna bases in sequence . its technologies are used in dna sequencing , diagnostics , drug development , biomolecular interaction , ion channel screening , and defense applications . the company was formerly known as oxford nanolabs , ltd . and changed its name to oxford nanopore technologies ltd . in may 2008 . oxford nanopore technologies ltd . was founded in 2005 and is based in kidlington , the united kingdom .

oxford nanopore technologies ltd is a private company headquartered at the oxford science park outside oxford , uk , with satellite offices in cambridge ( uk ) , new york and boston , and personnel also based in japan and france .

oxford , england - - ( business wire ) - - oxford nanopore announces £70 million fundraising to support develo . . .

oxford nanopore technologies was spun out from the university of oxford in 2005 . until may 2008 , the company was named oxford nanolabs ltd . oxford nanopore employs a team of more than 300 people including scientists , engineers , informaticians , manufacturing and commercial specialists . headquartered in oxford , uk , the company also has employees in cambridge ( uk ) , new york and boston .

are we heading for a minion movement in healthcare ? clive brown , cto of oxford nanopore technologies , talks to pharmatimes about the internet of living things .

oxford nanopore currently has 1 , 779 fans on facebook . as of august 2014 oxford nanopore had 155 fans . that ' s a 1047 percent increase in 4 years

© 2008 - 2018 oxford nanopore technologies . all rights reserved . registered office : gosling building , edmund halley road , oxford science park , ox4 4dq , uk | registered no . 05386273 | vat no 874 642494 | privacy policy | cookie policy | terms & conditions . oxford nanopore technologies , the wheel icon , gridion , metrichor , flongle , minion , minknow , promethion and voltrax are registered trademarks of oxford nanopore technologies limited in various countries . flongle , minion and promethion are currently for research use only .

since oxford nanopore was founded in 2005 , it has participated in 11 rounds of funding . in total oxford nanopore has raised $ 658 . 4m . oxford nanopore ' s last funding round was on mar 2018 for a total of $ 140 . 0m

oxford nanopore is organising its first conference , a two day event ) on all things nanopore in london , aptly named as london calling . among the many interesting talks , the most anticipated talks is from oxford nanopore cto clive brown’s talk announcing the future things to expect from oxford nanopore .

supported by a broad patent portfolio , the university - of - oxford nanopore pipeline includes multiple generations of nanopore - based sensing technologies , including those based on both biological and solid - state nanopores .

sara goodwin of coldspring harbour laboratory talks to the oxford nanopore community at london calling .

oxford nanopore ' s minion sequencer can read dna fragments up to 10 kilobases long .

oxford nanopore currently has 18 , 771 followers on twitter . as of august 2014 oxford nanopore had 6 , 609 followers . that ' s a 184 percent increase in 3 years

the minion ( aka biomolecule sequencer ) , a miniaturized dna sequencer , by oxford nanopore technologies is 3 ¼ × 1 ¼ × 5 / 8 inches and has a mass of 120 grams . the sequencer is powered via usb connection to a laptop or tablet and does not require a battery . image courtesy of oxford nanopore technologies .

nanopore devices can be adapted for direct sequencing of dna or rna . supported by a broad patent portfolio , the oxford nanopore pipeline includes multiple generations of nanopore - based sensing technologies , including those based on both biological and solid - state nanopores .

results from oxford nanopore ' s minion are promising , but fall short of high expectations .

oxford nanopore offers a range of options for converting your original biological sample to a form ready for application into a nanopore sensing device .

in october 2013 oxford nanopore raised £40m from new and existing investors in the us and europe .

in august 2014 oxford nanopore raised £35m from new and existing investors in the us and europe .

in july 2015 oxford nanopore raised £70m from new and existing investors in the us and europe .

this study aimed to assess the feasibility of using the oxford nanopore technologies ( ont ) minion long - read sequencer in reconstructing fully closed plasmid sequences from eight enterobacteriaceae i . . .

a sample being loaded into a flow cell attached to the minion . the minion is powered through a usb cable connected to a laptop or tablet . image courtesy of oxford nanopore technologies .

in september 2016 , oxford nanopore held a technical update , announcing new product upgrades to the minion .

new york ( genomeweb ) – oxford nanopore technologies yesterday provided several updates on its technology development and commercialization plans , including a new minion flow cell , called flongle , for clinical diagnostic applications .

a tiny new device called the minion , developed by oxford nanopore technologies , promises to help scientists sequence dna in space . nasa ' s biomolecule sequencer investigation is a technology demonstration of the device .

the oxford nanopore technologies ( ont ) minion is a new sequencing technology that potentially offers read lengths of tens of kilobases ( kb ) limited only by the length of dna molecules presented to it .

biotech firm oxford nanopore has raised £100m from its latest private funding round , valuing the rapi . . .

to assess the performance of the oxford nanopore technologies minion sequencing platform , cdnas from the external rna controls consortium ( ercc ) rna spike - in mix were sequenced . this mix mimics mamm . . .

in february 2010 oxford nanopore raised £17 . 4m from existing investors and new , undisclosed us - based investors .

motivation : the highly portable oxford nanopore minion sequencer has enabled new applications of genome sequencing directly in the field .

nabsys is oxford nanopore ' s # 2 rival . nabsys was founded in 2004 in providence , rhode island . nabsys operates in the health care services industry . nabsys generates $ 849 . 8k more revenue than oxford nanopore .

pacbio is one of oxford nanopore ' s top competitors . pacbio was founded in 2000 , and its headquarters is in menlo park , california . pacbio competes in the biotechnology field . pacbio has 343 more employees than oxford nanopore .

background the minion™ is a new , portable single - molecule sequencer developed by oxford nanopore technologies . it measures four inches in length and is powered from the usb 3 . 0 port of a laptop comp . . .

genia is a top competitor of oxford nanopore . genia is a private company that was founded in santa clara , california in 2009 . genia is in the health care services industry . genia has 83 more employees vs . oxford nanopore .

the minion is a portable single - molecule dna sequencing instrument that was released by oxford nanopore technologies in 2014 , producing long sequencing reads by measuring changes in ionic flow when single - stranded dna molecules translocate through the pores .

does oxford nanopore even use mspa in its sequencers ? very few people are in a position to know ; oxford nanopore does not disclose the makeup of its nanopores . the company was founded on the work of hagan bayley , a biochemist at the university of oxford , whose published research has mostly concerned a different nanopore - forming protein , α - hemolysin . but many different proteins have been suggested as promising nanopore materials , and oxford could easily have changed course during the minion’s development .

the oxford nanopore technologies minion sequencer enables the selection of specific dna molecules for sequencing by reversing the driving voltage across individual nanopores . to directly select molecules for sequencing , we used dynamic time warping to match reads to reference sequences .

oxford nanopore has raised â100m ( $ 140m ) to expand its product range including a device for food and . . .

san diego - - ( business wire ) - - feb . 23 , 2016 - - illumina , inc . ( nasdaq : ilmn ) today announced that the company filed lawsuits against oxford nanopore technologies ltd . and oxford nanopore technologies , inc . ( ont ) . the lawsuits were filed concurrently with the u . s . international trade commission and in the u . s . district court for the southern district of california . illumina is seeking all available remedies .

oxford nanopore has employees and is the third highest among it ' s top 10 competitors . the top 10 competitors average 116 .

a clever combination of technologies makes it faster and more secure to buy things with a wave of your phone .

genome assemblies obtained from short read sequencing technologies are often fragmented into many contigs because of the abundance of repetitive sequences .

deamer first came up with the idea for nanopore sequencing in 1989 when he was at uc davis . at uc santa cruz , he and akeson established the nanopore research group in 1996 , making important advances in nanopore sequencing technology in the subsequent decades . they both serve on oxford nanopore ' s technology advisory board .

36 . © copyright 2016 oxford nanopore technologies ⎪ 36 current work testing automated ansible scripts to deploy server and mongodb instances . locust to throw lots of data to deployed webserver nodes month of data in five minutes migration to 3 . 0 ( with wiredtiger )

13 . © copyright 2016 oxford nanopore technologies ⎪ 13 usb powers device , link to laptop or computer to operate consumable flowcell contains nanopore sensing chemistry and custom electronics sample added to flowcell here . currently this is prepared dna , designed to work with blood / tissue sensor chip works with custom asic for control and data processing

asic each nanopore channel is controlled and measured individually by the bespoke asic . this allows for multiple nanopore experiments to be performed in parallel . more than one asic may be included in a device and oxford nanopore is building asics of different sizes for different purposes .

in october 2018 , oxford nanopore announced its entry into the chinese market , having appointed a distributor and established its first sales to customers .

the existing sequencing technologies , and whole genome sequencing ( wgs ) performed mainly at centralised facilities , will become a niche .

oxford nanopore ' s revenue is the third highest among it ' s top 10 competitors . the top 10 competitors average $ 1 . 4m .

as one of the first research institutes to take part in the minion access programme ( map ) for portable dna sequencing , introduced by oxford nanopore technologies , the genome analysis centre ( tgac ) ' s task force share their experience of the ground breaking trial so far .

we report a rapid , inexpensive , and portable strategy to re - identify human dna using the minion , a miniature sequencing sensor by oxford nanopore technologies . our strategy requires only 10 - 30 minutes of minion sequencing , works with low input dna , and enables familial searches .

in june 2017 , oxford nanopore launched its rna sequencing solutions . this provides the only direct , real time rna sequencing technology and additional cdna analysis .

oxford nanopore delivers continual improvement where all parts of the technology are shipped , improved and developed . the format of the hardware , software and chemistry changes on a regular basis ( often over weeks rather than months ) . this iterative improvement process will continue throughout the lifetime of all oxford nanopore products .

glad to see oxford nanopore pushing the pace in our fair state ( california ) . at least five promethions in the san francisco bay area alone .

a good first shot , but not a game - changer — yet . that seems to be the consensus among scientists after today ' s first public release of data produced by the minion , an advanced and highly anticipated dna - sequencing device developed by uk company oxford nanopore technologies .

the talk did not seem to disappoint and clive brown announced a flurry of things to expect from oxford nanopore , minion asic , voltrax , and promethion .

* update , 11 march , 12 : 35 p . m . : oxford nanopore ' s response to illumina’s patent suite was added to the story .

oxford nanopore devices are fully scalable , based around a core sensing unit of a nanopore set in an arrayed sensor chip and used with a bespoke application - specific integrated circuit ( asic ) which controls and measures the experiments .

" a nanopore is a nano - scale hole . in its devices , oxford nanopore passes an ionic current through nanopores and measures the changes in current as biological molecules pass through the nanopore or near it . the information about the change in current can be used to identify that molecule . "

translating the oxford nanopore minion sequencing technology into medical microbiology requires on - going analysis that keeps pace with technological improvements to the instrument and release of associated analysis software .

in this talk i will cover the highs and lows of being part of the oxford nanopore minion access programme . our laboratory joined the map programme in may 2014 .

oxford nanopore was founded on the science of professor hagan bayley of the university of oxford . in 2008 the company created a series of collaborations with world - leading nanopore researchers at other institutions including harvard , university of california santa cruz and boston university . further collaborations have since been added ; these , in combination with in - house expertise and intellectual property give the company a leading position in nanopore technology .

oxford nanopore technologies aims to disrupt the paradigm of biological analysis . our technology and commercial model has already opened up dna analysis to researchers who previously had no direct access to sequencing technologies , freeing them up to perform analyses in their own labs or in the field , and in real time . we continually improve the technology performance , make it easier to use and expand the ways in which users can access nanopore sequencing . this technology pathway is designed to enable the analysis of any living thing , by any person , in any environment .

loman is a member of the minion access programme ( map ) and has received free - of - charge reagents for nanopore sequencing presented in this study . he has ongoing research collaborations with oxford nanopore but does not receive financial compensation for this .

in october 2016 , oxford nanopore announced that the early queue for the promethion early access programme was now full and announced transition to a more mature phase of the programme .

at this stage , oxford nanopore is not seeking additional investment . however , if you are interested in receiving information at the next opportunity then please use our contact form .

“it is gratifying , ” he said , “to have the commercial relevance of oxford nanopore products so publicly acknowledged by the market monopolist for [ next generation sequencing ] . ”

like many of oxford’s ideas , promethion is a work in progress . and it’s already well behind schedule . oxford now plans to start selling it later this year . “just sit tight , ” brown told scientists at oxford’s meeting . “the raw horsepower is enough to put you ahead . ”

clive g brown , cto of oxford nanopore , gave a technical update on 29 th september . if you missed it , you can watch it by registering your details here .

for his part , oxford nanopore ceo gordon sanghera , in a brief statement released today , said that illumina’s lawsuit is without merit and will not affect his company’s commercial prospects .

this work was funded in part by the wellcome trust , rosetrees trust , the biotechnology and biological sciences research council ( bbsrc ) , the canadian institutes of health research , the us national science foundation , the national institutes of health and the national human genome research institute ( nhgri ) . reagents were supplied by oxford nanopore technologies ltd .

nanopore systems will be developed that can also write dna , driving synthetic biology .

what is abundantly clear from looking through oxford’s intellectual property is that nanopore sequencing took a truly distributed effort to get off the ground . scientists from numerous universities in the us and the uk have played significant roles in bringing the world a working nanopore sequencer . so has the us government , through grants from the national human genome research institute and direct contributions from members of the national institute of standards and technologies . what oxford has done―and what illumina will have done , if it ever invents a nanopore sequencer of its own―is run the last , steepest mile , to engineer an instrument reliable enough for use in the real world .

biomolecular engineer mark akeson describes the development of nanopore sequencing technology at uc santa cruz .

oxford nanopore takes a different approach by using direct electronic sensing made possible by biological pores rather than sequencing chemistry , and could be a game changer in the field . the company was founded by

second and third generation sequencing technologies have revolutionised bacterial genomics . short - read illumina reads result in cheap but fragmented assemblies , whereas longer reads are more expensi . . .

enzyme motor : e7 to e8 . dna can be passed through the nanopore more rapidly , going from 250 bases per second per nanopore ( bps ) to 450 without losing accuracy

on 8th feb 2018 , clive brown , cto of oxford nanopore , gave an update on oxford nanopore technology . in this update he described the pathway to a sub $ 1000 genome using the promethion device , introduced ' flongle ' - a smaller flow cell for smaller experiments and tests , outlined the pathway to very high consensus accuracy , and some other goodies . . watch for more . show less

in may 2017 , r9 . 5 was shipped , to be compatible with the new 1d squared method of sequencing . oxford nanopore is at this stage producing more than 20gb from a single flow cell .

npreader ( jsa . np . f5reader ) is a program that extracts oxford nanopore sequencing data from fast5 files , performs an initial analysis of the date and streams them to real - time analysis pipelines .

a revolution is occurring in genomic epidemiology . recently , real - time portable genome sequencing using the oxford nanopore minion device was successfully used to characterize the genetic diversity of the ebola virus outbreak in guinea .

in november 2015 , minion users gathered at the first nanopore community meeting in new york .

motivation : the oxford nanopore minion sequencer , currently in pre - release testing through the minion access programme ( map ) , promises long reads in real - time from a cheap , compact , usb device .

it is time to shake up public health surveillance . new technologies for sequencing , aided by friction - free approaches to data sharing , could have an impact on public health efforts .

the minion device by oxford nanopore is the first portable sequencing device . minion is able to produce very long reads ( reads over 100 ~ kbp were reported ) , however it suffers from high sequencing error rate .

40 . © copyright 2016 oxford nanopore technologies ⎪ 40 zika virus research urltoken • how and when zikv was introduced to brazil • pattern and determinants of spread • extent of genetic diversity ( of importance to vaccine and diagnostic design ) , • associations between changes in the virus genome and • likelihood of zikv complications such as microcephaly . • provide a surveillance framework for tracking further spread into other geographic regions

metrichor was founded to offer fully integrated , bespoke , real - time analysis solutions based on scalable , real time nanopore sensing technologies . those complete solutions may include bespoke devices to extract a sample of interest , perform dna / rna sequencing or integrated software to provide analysis and actions .

the company now has more than 350 employees from multiple disciplines including nanopore science , molecular biology and applications , informatics , engineering , electronics , manufacturing and commercialisation . the management team , led by ceo dr gordon sanghera , has a track record of delivering disruptive technologies to the market .

illumina reserves the right to make nanopore sequencers in which dna passes through mspa or its derivatives .

motivation : the minion device by oxford nanopore is the first portable sequencing device . minion is able to produce very long reads ( reads over 100 ~ kbp were reported ) , however it suffers from high sequencing error rate .

metrichor ltd is a company formed by oxford nanopore . it offers end - to - end analysis solutions coupled with nanopore sensing devices , with the goal of making analyses accessible to people without bioinformatics skills or even biology qualifications . this may include ' applied markets ' such as food / environmental testing , or future consumer genetics applications .

illumina has plenty of competitors besides oxford nanopore . but while companies like pacific biosciences , thermo fisher , and qiagen all make machines that fill useful niches , they’re just nibbling at the edges of the genomics market . oxford―despite lingering problems with accuracy , and a history of delivering spectacularly behind schedule―is the only firm that looks like it might be an existential threat .

the band of aquanauts — including members of nasa and the european space agency ( esa ) — participated in a 16 - day underwater expedition known as the nasa extreme environment mission operations ( neemo ) 21 . the aquanauts first tested the palm - size minion dna sequencer , built by oxford nanopore technologies , underwater to " minimize unknowns and see how the device worked in various extreme environments , " nasa officials said in a statement .

david deamer ( left ) and mark akeson ( right ) established the ucsc nanopore research group in 1996 .

the basic idea behind nanopore sequencing has been floating around academia since the late 1980s . scientists including david deamer and mark akeson of the university of california , santa cruz , and george church of harvard , filed a series of patents related to nanopore sequencing as early as 1995 . oxford nanopore , which licenses these patents , was formed a decade later , as early prototypes in university labs began to show progress in turning the idea into a workable instrument .

24 . © copyright 2016 oxford nanopore technologies ⎪ 24 sepsis 1 / 3 patients in intensive care never leave hospital deaths per year in us > 200k ( more than prostate , breast cancer and aids combined ) . cost to us > $ 20 billion early diagnosis and evidence based treatment could è 92k fewer deaths ( us ) diagnosis window for septic shock : 6 hours minion being explored for early identification of infection ( uti / sepsis ) by uk group

brown joined oxford nanopore in june 2008 , having formerly worked at the uk ' s largest genomic laboratory , the wellcome trust sanger institute . he contributed to the development of more traditional dna sequencers , huge machines that cost a great deal to run , require special equipment and are centrally located in labs . nanopore sequencing , bypasses many of those problems .

" this is a wondrous technical achievement by oxford nanopore and nasa . i am proud of ucsc ' s key role in helping build the foundation that made it possible , " said mark akeson , professor of biomolecular engineering at uc santa cruz .

compare : illumina has 7 , 500 sequencers installed . oxford has placed 3 , 000 minion ( # in use currently not given ) urltoken

clive brown , the company ' s chief technology officer , presented the updates last night at the firm ' s user meeting in london . while his talk was not public , oxford nanopore disseminated much of the information through a series of twitter messages .

in addition to studying metagenomics , the tgac map team are also interested in understanding new technologies such as the minion and providing the scientific community with tools to help them get the most out of these technologies . the research group will continue to be part of the map and will be developing this work further by looking at other research areas for the minion , with a view to paper publication , tools releases , collaborations and grant applications .

two weeks ago i mentioned on twitter that i pushed a large series of changes to support r9 oxford nanopore data in nanopolish . i promised a blog post describing the changes then went off on my summer vacation - here it is , a little late .

in february 2012 at the agbt conference , oxford nanopore presented a variety of nanopore dna ' strand sequencing ' and protein - analysis data , and an overview of the hardware and software behind the gridion and minion systems . these data included small genomes that had been sequenced using the company ' s technology over the sense and antisense strands , showing tens of kilobases in single reads . this was the first nanopore sequence data to be shown worldwide since the technique was first theoretically proposed in 1996 .

the recently introduced oxford nanopore minion platform generates dna sequence data in real - time . this opens immense potential to shorten the sample - to - results time and is likely to lead to enormous benefits in rapid diagnosis of bacterial infection and identification of drug resistance .

“this reminds me of the early days of illumina , when all we could get were 36 - base - pair reads , and we were all very excited about that , ” says erlich . “i think we should give [ oxford nanopore ] more time . ”

nanopore reader : r9 to r9 . 4 . 1200 r9 mutants have been screened ; this is faster and more accurate

hi fabricio , the minion is made by oxford nanopore – urltoken in this study , researchers in several labs around the world tested out the performance of the device . embl - ebi coordinated the study , but we do not distribute the device . kind regards , mary

oxford nanopore provides signal processing and base - calling tools , but file formats are documented and participants can write their own data analysis tools or modify the scripts provided in the github repository . sources for any software are provided on an ‘as - is’ and ‘without warranties’ basis

the top 10 competitors in oxford nanopore ' s competitive set are pacbio , nabsys , genia , illumina , quantumdx , discoverx , diagenode , editas medicine , integenx and applied biosystems . together they have raised over 1 . 0b between their estimated 7 . 2k employees .

more recently , in 2013 illumina licensed patents from jens gundlach of the university of washington and michael niederweis of the university of alabama , birmingham , to pursue an in - house nanopore program . it is these patents that are now the object of illumina’s lawsuit against oxford .

bywaters kf , mckay cp , davila af , quinn rc . in situ life and biosignature detection at mars analog sites using the oxford nanopore minion sequencer . biosignature preservation and detection in mars analog environments conference . may 16–18 , 2016 ; no . 1912 , id 2014 .

in july 2009 , the company relocated to the oxford science park . our premises at edmund cartwright house were inaugurated by the uk science and innovation minister , lord drayson . in 2011 , an additional 7 , 000 square feet on the oxford science park was opened and a new cambridge office was also opened .

the convergence of automation technologies , intelligent algorithms and cloud computing is progressively making genomics available to less skilled actors . while this does not necessarily ensure democratization , it does enable us to imagine it . and so , what if it actually happens ?

array of microscaffolds each microscaffold supports a membrane and embedded nanopore . the array keeps the multiple nanopores stable during shipping and usage .

dr andrew kilianski presents a talk for the minion community on real - time genomic characterization of viral threat agents using nanopore sequencing .

the suit came as distressing news to some researchers , who feared they might lose access to the firm’s nanopore products , or that the battle would stifle the development of new platforms . “i would love it if illumina had released a platform . instead what they’re doing is releasing a lawsuit , ” says winston timp , a biomedical engineer at johns hopkins university in baltimore , maryland , whose lab has also licensed patents to oxford nanopore .

oxford nanopore has developed voltrax – a small device designed to perform library preparation automatically , so that a user can get a biological sample ready for analysis , hands - free . voltrax is designed as an alternative to a range of lab equipment , to allow consistent and varied , automated library prep options .

if the ambition is to promote more distributed use of genomic sequencing , users also need a ready - made platform for interpreting genetic data . oxford nanopore has designed an intelligent cloud lab , metrichor , to be used for genomics data storage in conjunction with smartphone apps that interpret the meaning of dna sequences .

nanopore devices perform dna / rna sequencing directly and in real time . the technology is scalable from miniature devices to high - throughput installations .

people will develop and commercialise their own assays on the nanopore platform , for their own applications , rather like programming a new iphone app .

other researchers will soon have a chance to form their own opinions about the device , as oxford nanopore today launched its early - access programme . researchers who pay a us $ 1 , 000 deposit , plus $ 250 for shipping costs , can receive their own minions to begin testing the machines in their labs .

even if oxford does have mspa - based nanopores inside the minion and promethion , illumina’s lawsuit is far from a slam dunk . oxford holds or licenses a wide array of intellectual property―some from its co - founder bayley , but also from deamer , akeson , church , and many others . these patents cover the foundational concept of nanopore sequencing ; the engineering of protein nanopores ; and other aspects of the technology not at stake here , like the enzymes that help guide dna through the system .

antonio regalado on twitter : " compare : illumina has 7 , 500 sequencers installed . oxford has placed 3 , 000 minion ( # in use currently not given ) … "

in nanopore sensing , just as water flows through a drain , an ionic current flows through a nanopore . when molecules pass through the hole the current is disrupted in characteristic ways , similar to the way the water flow would change if you placed objects in the drain credits : urltoken

" nanopore sequencing is an ideal example of how academic scientists can collaborate with businesses to develop new instruments with unprecedented capabilities , " deamer said . " clive brown , who led the development of the minion at oxford , suggested that it will make possible an internet of living things . that sounds right to me . "

average read length almost 7kb . 330mb events and 46k reads in 3 . 5 hours and still going well . excellent work @ nanopore ! !

yes expected , main reason is error in nanopore data , other is this is a clinical isolate and we don ' t have a reference .

these “nanopore” techniques , originally proposed by hagan bayley , a professor of chemistry at oxford , in 1992 , are simple , at least in principle . they do not need fluorescent labels and will probably not require amplification , either . they can “read” dna directly and rapidly , and are cheap enough to replicate in vast numbers .

thanks to matei david , jonathan dursi , nick loman , josh quick and phil zuzarte for helping develop the r9 support and generating data . the scientists at oxford nanopore helped us better understand r9 data and the discussions we had with them were key to implementing the improved hmm - thanks in particular to clive and chris wright .

a review of the nanopore technology platform , which is designed to enable the analysis of any living thing , by any person , in any environment .

the minion , a handheld dna - sequencing device developed by oxford nanopore , has been tested and evaluated by an independent , international consortium coordinated by embl - ebi . the innovative device opens up new possibilities for using sequencing technology in the field , for example in tracking disease outbreaks , testing packaged food or the trafficking of protected species .

metrichor ltd offers end to end analysis solutions , using real - time , miniaturisable and scalable dna / rna sequencing technology from oxford nanopore . designed for real time surveillance of complex systems such as food supply chains , environments or outbreaks , to single locations such as industrial or medical diagnostics , metrichor can design bespoke solutions for rapid insights .

in may 2015 , the first nanopore sensing conference was convened ( london calling ) where users of minion technology gathered to hear from 20 speakers and additional abstracts from numerous other map participants across a range of applications . minion became commercially available at this time and the minion access programme became the nanopore community .

the dna sample ( metagenomics gdna from lake hillier xmp project ) was sheared to 10 kb size using a covaris g - tube ( figure 2d , supplementary figure 4 ) . dna was then prepared using the oxford nanopore r9 protocol ( version nsk - 007 ) , including ffpe repair , end repair and da - tailing , adapter ligation and tethering , and streptavidin recovery . the prepared library was run on an r9 minion flowcell using minknow version 0 . 51 . 1 . 66 with the “nc _ 48hr _ sequencing _ run _ flo _ min104 . py” protocol . the raw data were called with metrichor using “2d basecalling rnn for sqk - nsk007” version 1 . 99 ( oxford science park , oxford , uk ) .

minion™ is a memory stick - sized , nanopore - based sequencer primarily designed for single - molecule sequencing of long dna fragments ( > 6 kb ) .

a webcast presentation today for customers of the company will likely put many of those fears to rest . oxford nanopore’s chief technology officer clive brown announced an upgrade to its devices—a new pore the company has been describing in presentations as r9 . brown revealed that r9 is csgg , a membrane protein derived from escherichia coli , but present in many bacterial species .

and it’s not all about dna . bayley says oxford nanopore was founded as a company interested in developing single molecule sensing technology for metabolites , sugars and other compounds of biochemical interest . " we saw it as a platform technology . but quite soon it became obvious that its application in third - generation dna sequencing was an area with enormous potential . "

whether illumina is narrowing in on its own nanopore sequencer is a mystery . the company did receive a 2014 grant from the national human genome research institute related to a nanopore project ; this seems to be the most recent public evidence that the program is active . it is suggestive that illumina has recently been touting a

“nanopore sequencing will open the door for the development of novel tools and applications to analyse the influx of new data , ” says rebecca lawrence , managing director of f1000research . “the nanopore analysis channel on f1000research will be a central , open platform on which scientists can publish and discuss new applications and analyse workflows for nanopore sequencing data . people can access and contribute data easily , so that the wider life - science community can realise the full potential of this new technology quickly . ”

we provide the latest information on how to use the products via the nanopore community website , which also serves as a forum for minion users to share information .

eleonore pauwels is a writer and international science policy expert , who specializes in the governance of converging technologies , including artificial intelligence , genomics , digital bio - engineering , participatory health design , and citizen science . at the wilson center , she is the director of the ai lab within the science and technology innovation program .

in may 2016 , the minion mk 1b was introduced . preparing for future iterations of nanopore chemistry it included improvements such as greater temperature control of the flow cell .

minion was launched into the minion access programme in spring 2014 and made commercially available in may 2015 . the nanopore community has grown around the two phases of minion availability .

a strand of dna is passed through a nanopore . the current is changed as the bases g , a , t and c pass through the pore in different combinations .

in the case of oxford nanopore ' s technology , the dna molecule is fed through the hole one base at a time by a second protein , an enzyme called an exonuclease . this can clearly distinguish the four dna letters , a , c , g and t . it may also be able to detect whether a letter is methylated , though this has not yet been independently verified .

jaqueline goes de jesus of the oswaldo cruz foundation and nuno faria of the university of oxford use the minion sequencer in joão pessoa , brazil , as part of a mobile study on the origins of the zika virus .

the desk in gordon sanghera ' s office at oxford nanopore proudly displays a piece of knobbly plastic . it has a hole running through it and looks rather like a cruller doughnut , though with grey icing . in fact , it is a model of a protein molecule called alpha - hemolysin . dr sanghera , the firm ' s boss , believes this molecule will revolutionise the sequencing of dna .

nanopolish for @ nanopore r9 data has been released . consensus accuracy is up to 99 . 9 % ( deep e . coli data ) . details in a upcoming blog post

nanopore dna strand sequencing has emerged as a competitive , portable technology . reads exceeding 150 kilobases have been achieved , as have in - field detection and analysis of clinical pathogens .

but isaac ro , an analyst at us investment bank goldman sachs — which advised illumina in 2012 when it fought off a takeover bid by the swiss drug giant roche — called jaffe ' s presentation “underwhelming” . “oxford nanopore still appears to be in development mode and , in our view , is unlikely to threaten [ illumina’s ] competitive position at this time , ” ro said in a research note .

in december 2016 , the second nanopore community meeting was held . during this meeting , three groups presented or released t he first human genomes to be sequenced on the handheld minion .

. . . and now for something completely different . new tools for diet analyses : nanopore sequencing of metagenomic dna from stomach contents to quantify diet in an invasive population of rats with

" the fundamental research and patents underlying the technology of nanopore sequencing were carried out here at ucsc , " said david deamer , research professor of biomolecular engineering at uc santa cruz .

i have received an honorarium to speak at an oxford nanopore meeting , and travel and accommodation to attend london calling 2015 and 2016 . i have ongoing research collaborations with ont although i am not financially compensated for this and hold no stocks , shares or options . ont have supplied free - of - charge reagents as part of the minion access programme and also generously supported our infectious disease surveillance projects with reagents .

we are sending up samples that we are preparing on the ground , and the crew will thaw the samples and load them for sequencing . the samples will contain lambda bacteriophage , which is oxford nanopore technology ' s characterization standard for sequencer performance . the samples will also contain e . coli and mouse genomic dna to demonstrate that you could sequence dna from any organism . the samples will not contain human dna .

in february 2017 , the gridion x5 was announced ; a desktop system integrating five minion flow cells with integrated compute function , that can be used to offer nanopore sequencing as a service .

a protein nanopore is set in an electrically resistant polymer membrane . an ionic current is passed through the nanopore by setting a voltage across this membrane . if an analyte passes through the pore or near its aperture , this event creates a characteristic disruption in current ( as shown in the diagram below ) . measurement of that current makes it possible to identify the molecule in question .

nanopore sequencing introduces true real - time sequencing for the first time . full exploitation of real - time sequencing requires a novel approach to data analysis for which we have developed the minotour platform .

the average length of the sequences generated on the minion is 5 . 4 kilobases , with some as long as 10 kilobases . that is longer than the average read out delivered by the current dominant sequencing technology , sold by illumina of san diego , california , which delivers fragments of dna that are hundreds of base pairs long . but the median length of the minion’s reads is shorter than the target that oxford nanopore announced in 2012 .

all those activities might one day be run instead using nanopore sequencing — or at least oxford hopes so . right now , the minion is a good way to identify and study bacteria and viruses . but last year , the first human genomes were sequenced on a minion as well . ( it’s still a cumbersome process and not as inexpensive , however — taking close to $ 20 , 000 worth of disposable cartridges at $ 500 each . )

30hrs , ~ 14gb and 450 in strand with 1263 active pores still . loving these new revd flowcells . home it came today so i can nurture it along over the weekend . @ nanopore urltoken

the minion is not yet for sale . but david jaffe , a computational biologist at the broad institute in cambridge , massachusetts , used data produced by the device and provided by oxford nanopore to aid in the assembly of two bacterial genomes — those of escherichia coli and a bacterium from the genus scardovia that is found in the human mouth . he presented his results today at the advances in genome biology and technology meeting in marco island , florida .

illumina is improving human health by unlocking the power of the genome . our focus on innovation has established us as the global leader in dna sequencing and array - based technologies , serving customers in the research , clinical and applied markets . our products are used for applications in the life sciences , oncology , reproductive health , agriculture and other emerging segments . to learn more , visit urltoken and follow @ illumina .

last month , josh quick , a bioinformatician and phd student working in my group at the institute of microbiology and infection , travelled to the epicentre of the outbreak in guinea . he took with him a portable genomic surveillance system carried in his hold luggage . the system contained several minions : portable “usb stick” sequencers , developed by the british company oxford nanopore , as well as all the chemicals and pipettes and plastic tubes that are needed to generate sequences of the ebola virus .

even closer to home , genome sequencing is becoming mainstream with the 100 , 000 genomes project . and armed with new technologies , it may soon be possible for individuals to sequence their own genomes and for infectious pathogens to be sequenced at home or in a gp surgery . when it comes to ebola , we hope the ability to provide portable , real - time sequencing will improve our ability to respond in future .

so that we can open up the use of nanopore analysis by any person and in any environment , we are developing a low cost consumable device for combined sample and library preparation . project zumbador is progressing well .

in my talk , i will discuss my collaboration with nick loman’s lab to develop de novo assembly methods for minion data . we have built a pipeline to error correct nanopore reads using partial order gr . . .

jared simpson on twitter : " nanopolish for @ nanopore r9 data has been released . consensus accuracy is up to 99 . 9 % ( deep e . coli data ) . details in a upcoming blog post "

but he sounded optimistic about the minion ' s future . oxford nanopore has said that higher - quality dna or different preparation methods should increase the average read length . jaffe says that the company can work to eliminate errors , perhaps by using a variety of pores with different properties . but even now , he says , most long minion reads had lengthy stretches without any mistakes . the systematic errors are “a temporary feature that we hope they ' ll figure out how to solve” .


mulholland books ( uk ) is an imprint of hodder & stoughton , a british publishing house , now a division of hachette uk .

it specialises in the suspense fiction genre including crime novels , thrillers , police procedurals , spy stories and supernatural suspense

mulholland books ( uk )

mulholland books ( uk ) <EOT> crime writer sam hawken is to move to mulholland books in a joint world english deal . ruth tross , editor at mulholland . . .

takes over duties as editor of the mulholland uk imprint and has her work cut out with a new list for a new year .

sage publications : london , uk , pp . 248 - 273 . isbn 9781412919913

1992 little , brown and company acquires macdonald & co . , renames it little , brown and company ( uk ) , and later time warner book group uk .

little , brown and company announced the creation of mulholland books on june 15 , 2010 . [ 1 ] mulholland books is named after mulholland drive , a winding stretch of road that follows the ridge line of the hollywood hills . [ 2 ] the imprint launched in spring 2011 with the publication of guilt by association by marcia clark . [ 3 ] the sister imprint mulholland books ( uk ) also launched at the same time .

hodder & stoughton is a major publisher within hachette uk , one of the uk’s biggest publishing groups . we publish a wide range of fiction and non - fiction titles and are renowned for passion , quality and delivering bestselling commercial books in many different formats .

with an exciting series of novels . though we have to also report that mulholland editor

series : anthropological studies of creativity and perception . ashgate : aldershot , uk , pp . 318 - 345 . isbn 9780754673743

our latest featured classic title from the mulholland backlist is savage season by joe r . lansdale .

mulholland books ( us ) is an imprint of little , brown and company , a division of the hachette book group . it specializes in publishing mysteries , thrillers , and suspense novels .

visit hodder books on pinterest to see what ' s been catching our eye , as well as visual inspiration , author guest boards , beautiful books and more .

award - winning publications , elegant gifts , children ' s books and toys .

little , brown and company announces the creation of a new imprint devoted to suspense , thrillers and crime fiction of all kinds . the imprint , later named mulholland books , will begin publishing titles in the spring of 2011 .

a new publishing division called hachette books is established on the foundation of the hyperion list .

novel suspects is a community of mystery and thriller fans who love to share our favorite books , movies , tv shows , and podcasts . but we love books most . check us out !

an imprint dedicated to suspense fiction . at mulholland , you never know what ' s coming around the curve . tweets by ruth , cicely , and hannah .

based in northumberland , bloodaxe books has an international reputation for publishing exciting poetry and excellently designed books . founded in newcastle in 1978 by neil astley , bloodaxe publishes , among others , jenny joseph and piotr sommer .

– this resource is regularly updated thanks to the work of mulholland’s hard working miriam parker . i would urge you to bookmark this superb site if you like crime and thrillers .

dr marc mulholland , review of the republic : the fight for irish independence , 1918 - 1923 , ( review no . 1516 ) urltoken date accessed : 9 july , 2018

when you create an account with us , you ' ll be able to save your favourite books , make a wishlist of upcoming titles , receive newsletters about books you ' ll love , get recommendations tailored to you and order our books directly . join us by creating an account and start getting the best experience from our website !

2016 hbg acquires the perseus books group , which becomes the perseus books division ( deal closes 4 / 1 / 2016 ) . perseus adds five main imprints : avalon travel , basic books , da capo press , publicaffairs , and running press , as well as offices in berkeley , ca ; boulder , co ; and philadelphia , pa .

founded in october 2005 by alessandro gallenzi and elisabetta minervini , alma books publishes a mixture of translations and english - language originals . producing 20 to 30 titles a year , mainly in the field of contemporary literary fiction , alma books places a strong emphasis on the physical quality of the books as well as the quality of the authors published .

the winkie’s diner scene in david lynch’s 2001 film mulholland drive has been slowly racking up thousands of views on youtube and gaining a reputation as one of the scariest film moments ever created .

2005 5 spot , an imprint of warner books publishing chick - lit that is smart , fun , and filled with attitude , is launched . lb kids , an imprint of little , brown books for young readers , is launched .

little brown and company announces the creation of a new imprint , lee boudreaux books , which will start publishing in 2015 .

founded in 1999 by nicola beauman , persephone books reprints forgotten classics by 20 - century ( mainly women ) writers . exquisitely bound , the books can be purchased online and at the persephone bookstore , based in bloomsbury , london . books range from leonard woolf ' s " the wise virgins " to kay smallshaw ' s 1949 classic " how to run your home without help . "

another excellent ebook by andrew mulholland . i was particularly interested to see if he would be able to condense churchill’s long and involved life into a manageable read , and he has done just that . there are many books and many thousands of pages written about churchill and although i am very familiar with churchill’s life this ebook is the one text i will keep to refer to . additionally andrew mulholland has furnished an excellent appendix giving brief biographies of many of the important , but peripheral , politicians of the time . churchill is too often considered as a solitary phenomenon , but his life is best seen within the context of the times in which he lived and the politics of the day . andrew mulholland has achieved this combination admirably in a remarkably compact ebook .

like us on facebook for the latest news , photos , and videos as well as exclusive competitions on your favourite books and authors .

mulholland drive is a winding stretch of road that follows the ridgeline of the hollywood hills . its hairpin turns and sharp cliff - faces are shrouded in secrecy and imbued with drama , making them synonymous with suspense . the mysteries of mulholland have inspired countless novels , films and works of art , from raymond chandler to michael connelly to david lynch . we ' ve taken those twists and deep shadows and created a list of books that build on that rich history , hurtling readers around those hairpin bends with a screech of tyres and a lurch of the stomach on every turn of the page .

subscribe to our youtube channel to see the latest about your favourite authors and books , including book trailers , exclusive author interviews , and more .

1996 little , brown and company and warner books merge to become time warner trade publishing , later to be renamed time warner book group ( twbg ) .

2007 warner books is renamed grand central publishing . warner wellness is renamed wellness central . warner business books is renamed business plus . yen press , a graphic novel and manga imprint , is launched . orbit , a science fiction and fantasy imprint , is launched . twelve , a new imprint of gcp , is launched . poppy , an imprint of little , brown books for young readers and home to the gossip girl an other teen fiction series , is launched .

club mulholland is a members club for driving enthusiasts - with members all over the world . this is their first magazine , with a look back over 2016 ' s events . next issue in july will feature members cars and future events . . .

our books are available through most booksellers . follow the link to find your local chain and independent bookshops - for browsing the good , old - fashioned way .

2014 hbg acquired publishing company black dog & leventhal , and it becomes an imprint under the hachette books division ( deal closes 12 / 31 / 2014 ) .

we constantly re - evaluate our understanding of readers to find new areas of the market and to bring readers the books they want , the way they want them .

a ray of hope for independents , edinburgh - based canongate books bought back their independence in 1994 and , under the management of jamie byng and david graham , became one of britain ' s most dynamic publishing houses . publishing fewer books and putting an emphasis on quality led them to books like yann martel ' s " life of pi , " which won the 2002 man booker prize . they have since gone from strength to strength and in 2005 outbid harpercollins and random house for neil strauss ' s " the game . "

deadly secrets ( 4 ) by britta bolt ( mulholland books ) is the latest in a crime trilogy set in amsterdam and featuring the playfully named pieter posthumus , whose lonely funerals team makes sure that even the nameless and friendless receive a decent burial . it’s written by britta böhler , a german former lawyer , and rodney bolt , who was born in south africa but is best known to telegraph travel writers for his writing on his adopted country of holland .

2006 twbg is acquired by hachette livre and renamed hachette book group usa . warner faith is renamed faithwords . time warner audio books is renamed hachette audio . warner wellness , an imprint of warner books focused on the healing and improvement of mind , body , and spirit , is launched . springboard press , a baby boomer generation targeted imprint , is launched .

the pbs great american read series celebrates the power , passion , and joy of reading through the lens of america ' s 100 best - loved books as chosen by the public .

founded in 1979 by alan and jane davidson and run , since 1993 , by tom jaine , prospect books is based in devon and publishes books about cookery , food history and the ethnology of food . prospect is one of the very few british publishers to specialize in this field and produces four to eight books a year . titles include the classic " a baghdad cookery book " by al - baghdadi ( translated by charles perry ) , and the upcoming " spices and comfits - collected papers on medieval food , " by johanna maria van winter ( to be published later this year . )

enter your mobile number or email address below and we ' ll send you a link to download the free kindle app . then you can start reading kindle books on your smartphone , tablet , or computer - no kindle device required .

ian buruma’s murder in amsterdam ( 8 ; atlantic books ) examines the killing of the filmmaker theo van gogh by a young muslim amsterdammer , but goes on to offer a superb , astute analysis of tolerance and the entire dutch liberal ethic in a multicultural society .

i am currently writing two books . ( 1 ) a history of the concept of the ' proletariat ' in political thought from the french revolution to the 1930s . ( 2 ) a life - story of emmanuel barthélemy ( 1820–1855 ) , an adventurer , revolutionary , and murderer .

the dinner ( 2 ) by herman koch ( atlantic books ) has not only been a huge hit for the dutch writer at home but has sold more than a million copies in europe . it tells the story of two middle - class families , each with a 15 - year - old son caught up in the murder of a homeless woman . his second book to be translated into english , summer house with swimming pool ( 3 ; atlantic books ) , is about a doctor to the rich and famous who is accused of murdering one of his patients .

city - pick amsterdam ( 9 ) , edited by heather reyes and victor schiferli ( oxygen books ) , is a great little anthology of all sorts of snippets – fiction and non - fiction , dutch and foreign , contemporary and historical – perfect reading for a holiday or a weekend break .

founded in march 2003 by emma barnes , rob jones and louis wustemann , snow books publish a good combination of modern and classic works - from robert finn ' s contemporary thrillers to the musings of a . a . milne . the company has a strong environmental policy while also publishing very handsome urltoken

marcia clark is a former los angeles deputy district attorney who was the lead prosecutor on the o . j . simpson murder case . she co - wrote a bestselling non - fiction books about the trial , without a doubt , and is a frequent media commentator and columnist on legal issues . she lives in los angeles .

there is so much complexity to churchill which has been captured succinctly by andrew mulholland . this captivating journey through the history of one of britain ' s most famous characters keeps the reader interested and informed . i knew little of churchill other than his world war 2 exploits but now have a wider and better understanding of the man himself and his drivers . the other helpful part of the book was the insight into the other key people of the era . overall an excellent and informative read which i would strongly recommend to anyone .

2011 the hachette nashville division announces the creation of a new imprint , jericho books - - a progressive , non - traditional faith - based imprint , to begin publishing in the summer of 2012 . 2012 grand central publishing announces the launch of forever yours , a new digital - only romance imprint , to begin publishing in february 2012 .

hachette book group was created when hachette livre , a global publishing company based in france , acquired time warner book group from time warner in 2006 . hachette livre is a wholly - owned subsidiary of lagardère , a company that is active worldwide in the areas of communications and media ( books , press , audiovisual and distribution / retailing of cultural products ) .

publishing in the early years included books from winston churchill , jm barrie and gk chesterton , and the bible . the 1920s ' yellow jacket ' series were the precursors to the first paperbacks and included bestsellers from john buchan , sapper and edgar wallace . in 1921 hodder & stoughton also took over ownership of the soon - to - be world - famous medical journal ,

the happy owners of the harry potter empire , bloomsbury realized the small publisher ' s dream in 1997 when they plucked the boy wizard out of obscurity and made him a world name . founded in 1986 on the principle of bringing books of the highest quality to the mass market , notable authors include carlos fuentes , t . c . boyle and , of course , j . k . rowling .

founded in 2001 by martin rynja , gibson square publishes new titles as well as modern classics under their new editions imprint . their points of interest are books with a strong biographical element and an ability to provoke or contribute to current debate . publications include " no war , " co - written by naomi klein , and craig unger ' s controversial " house of bush , house of saud . "

if you don ' t want to get bogged down in a chunky book taking up half your bookshelf then this is an ideal whizz through the life and times of winston churchill . lots of facts to absorb with so much detail and relatively little padding for an interesting read , rather like an exam revision book i suppose . if you wanted to go into more depth with more heavier books then this would be a good way to get started .

founded in 1999 by melissa ulfane , pushkin press publishes classics and contemporary european literature in translation . based on the french livres de poche , the books are beautifully bound and typeset . stephen zweig and soren kierkegaard are among the authors published . upcoming translations , to be published this year , include florian zeller ' s " the fascination of evil " and erling kagge ' s " advice for polar explorers , or what they didn ' t teach you in school . " urltoken

hesperus press , founded in 2002 , established its name by publishing little - known works by classic authors , with a strong emphasis on the books ' appearance and introductions by modern literary luminaries . in may 2005 they published their first contemporary works . notable publications include " hyde park gate news " - the collected newpapers written by a 10 - year - old virginia woolf , with vanessa bell and thoby stephen ; alexander pope ' s " scriblerus , " and fyodor dostoevsky ' s " the double . " urltoken

founded in 1996 to publish " stimulating non - fiction , " profile is another plucky independent that found a well - deserved golden nugget with lynne truss ' s pedantic grammar rant , " eats , shoots and leaves , " in 2003 . in 2005 profile published about 60 books , " a sufficiently small number that everyone in the company can be involved in every book - but large enough to ensure that we are effective . " upcoming publications include francis fukuyama ' s " after the neocons , " in urltoken

founded in 1949 by john calder , calder publications began publishing translated classics before moving on to american titles . reaping profit fromsenator mccarthy ' s " witch hunts , " calder was able to acquire significant american authors whom many new york publishers were afraid to keep on their lists . in the 1950s calder published many of the writers that changed the face of european literature - including samuel beckett , alain robbe - grillet , marguerite duras and nathalie sarraute . resisting merger and takeovers , calder publications continues to publish challenging and intelligent books .

( 2001 ) , a new release from the criterion collection . it’s all bushes and darkness and bends in the road , various cars’ tail - lights appearing and disappearing . anything could happen there , especially since there are some very posh residences among the shrubs . but the view from mulholland drive is even more significant . ‘it could be someone’s missing maybe , ’ one detective says to another as they stand by the remains of a car crash on the drive . ‘that’s what i’m thinking , ’ the other detective says , and turns to look at the city from where they are standing : a whole world of bright lights flashing faintly far below . the detective walks to the edge of the cliff and stares down into the valley , as if the missing person has to be there , among the lights , along with the answer to every other question .

founded in 1986 by pete ayrton , serpent ' s tail was inspired by continental publishing houses ; publishing " paperback originals " instead of hardbacks . combining a mixture of original fiction , music and popular culture books , translations and themed short story anthologies , serpent ' s tail has established a strong reputation as a publisher of quirky and interesting works . notable authors include walter mosley , kenzaburo oe , alison fell and catherine millet - whose 2002 succès de scandale " the sexual life of catherine m . " was a profitable coup for the press .

luis buñuel is the great artist of the unflagged dream , the one you recognise for what it is when the character wakes up but not before , as happens repeatedly in the discreet charm of the bourgeoisie . but lynch is the champion of the long illusion : the dream sequence in mulholland drive takes up nearly two hours of a film that is just short of two and a half hours long . he is quite clear , as buñuel is , about when the dream ends . in a wonderfully swift and elliptical series of shots , two women return to an apartment ; one puts a small box on a bed , the other moves away to take a larger box from a cupboard , and when she turns back , her companion has gone . she calls , as if the other woman had to be still in the apartment somewhere ; but she doesn’t know what kind of movie she is in . her companion has left this story entirely and in a moment will be seen inhabiting a quite different one .

little , brown and company is one of the country’s oldest and most distinguished publishing houses . led by publisher reagan arthur , little , brown brings out fiction and nonfiction by many of america’s finest writers in hardcover , paperback , ebook , and audio formats . michael pietsch is the chairman and ceo of the hachette book group , of which little , brown is a division . terry adams , digital and paperback publisher , oversees little , brown’s paperback lines , including back bay books and little , brown trade and mass market paperbacks , as well as little , brown’s electronic publishing program . judy clain is little brown ' s editor in chief and craig young is its deputy publisher .

little , brown and company was founded in boston in 1837 , when charles little and james brown formed a partnership “for the purpose of publishing , importing and selling books . ” the company was primarily a bookseller at first , as were most publishing firms of that day , and has in fact traced its roots back even further – to 1784 and a bookstore opened on marlborough street by ebenezer battelle . mr . little and mr . brown began by publishing the works of benjamin franklin and george washington , and highlights of their early lists include edward gibbon’s decline and fall of the roman empire , the letters of john adams , the speeches of daniel webster , and oliver wendell holmes jr . ’s landmark the common law . in 1859 the company took over publishing rights to a book entitled familiar quotations by john bartlett . the eighteenth edition of the now familiar bartlett’s familiar quotations , with geoffrey o’brien as general editor , arrived in 2012 .

we ' ve detected that javascript is disabled in your browser . would you like to proceed to legacy twitter ?

this looks like a summer read i won’t be able to put down ! and just look at all those author endorsements thanks @ ruthtross for the best bookpost . can’t wait to read @ sabinedurrant ’s latest # takemein urltoken

- ' a tense and razor sharp psychological thriller that will keep you guessing . '

is out now and the perfect read for the sun lounger . just make sure you put plenty of sun cream on - once you start reading you won ' t be able to stop !

about my new book the death knock - set in a newsroom . . . .

is already a fan and we know you will be too . you can order your copy here :

couldn ' t settle on new audiobook . thought sod it , ' i ' ll go for take me in by

' even though i only read it a few months ago ! reasons 1 ) it ' s brilliant 2 ) rory kinnear and morven christie are reading it . . . i mean , c ' mon ! !

we ' ve read all 4 . they ' re terrific stories and capture the imagination ( and memories ) of mumbai and the people . great characters and stories . couldnt wait for murder at the grand raj palace to go to paperback . when is no . 5 coming out ? surely they would make a great tv series !

i was holding my breath till take me in by was finally released . and holding it all the way through . stunning writing and a satisfyingly twisty plot . thank you

yes ! and @ katemosse ! we are all hobnobbing around julie ' s left elbow . s ' marvellous !

! there i am , next to julie walter ' s left forearm and i can not think of a nicer place to be .

twitter may be over capacity or experiencing a momentary hiccup . try again or visit twitter status for more information .

you can add location information to your tweets , such as your city or precise location , from the web and via third - party applications . you always have the option to delete your tweet location history . learn more

here ' s the url for this tweet . copy it to easily share with friends .

by embedding twitter content in your website or app , you are agreeing to the twitter developer agreement and developer policy .

not on twitter ? sign up , tune into the things you care about , and get updates as they happen .

this timeline is where you’ll spend most of your time , getting instant updates about what matters to you .

hover over the profile pic and click the following button to unfollow any account .

when you see a tweet you love , tap the heart — it lets the person who wrote it know you shared the love .

the fastest way to share someone else’s tweet with your followers is with a retweet . tap the icon to send it instantly .

add your thoughts about any tweet with a reply . find a topic you’re passionate about , and jump right in .

prime members enjoy fast & free shipping , unlimited streaming of movies and tv shows with prime video and many more exclusive benefits .

after viewing product detail pages , look here to find an easy way to navigate back to pages you are interested in .

; which for a publisher is excellent . instead of a hard - sell of their own published authors , they feature interesting articles from

leaves at the end of the year for pastures new , as editorial director of orbit , the sf imprint .

los angeles d . a . rachel knight is a tenacious , wise - cracking , and fiercely intelligent prosecutor in the city’s most elite division . when her colleague , jake , is found dead at a grisly crime scene , rachel is shaken to the core . she must take over his toughest case : the assault of a young woman from a prominent family .

but she can’t stop herself from digging deeper into jake’s death , a decision that exposes a world of power and violence and will have her risking her reputation–and her life–to find the truth .

in which charlie hardie , ex - cop - turned - house - sitter , is about to have the worst day ever . his new job comes with a b - movie actress squatter who thinks that people are trying to kill her… unfortunately for charlie , she’s right . we’re publishing fun and games in june , with its sequels following in august and september . once you’ve read the first one , you’ll understand why we couldn’t make anyone wait for too long to find out what happened next ! it’s a real rollercoaster ride of a novel .

it’s a gritty , fast - paced thriller set on the us - mexico border . undercover cop valentine pescatore , us agent isabel puente and mexican police chief leo mendez have the same aim : to bring down the drug cartels . but in a world built on lies and corruption , how do you know who you can trust ? as pescatore gets drawn ever deeper into the criminal underworld , the stage is set for an explosive showdown .

is scheduled for november . leo , a disgraced former spy , and tasha , a lawyer grieving for a brother killed in iraq in mysterious circumstances , don’t realise the impact their actions will have on the world , but the man known as zed does . he’s been sent back from a future utopia with a single mission : to ensure that a cataclysmic event takes place which will ensure never - ending peace in his own time . but there may be things zed’s superiors haven’t told him… recalling dystopian classics like 1984 , the revisionists is a page - turning , mind - bending read .

newsy stuff - lee child : adventures of an over - rea . . .

you never know what ' s coming around the curve . like | follow | read

huffington post : you once described your father as a deep thinker and storyteller , a ‘black socrates . ’ will you elaborate a bit ?

walter mosley : if you were poor and white , you might have claimed socrates as an inspiration .

but if you were poor and black , there was nobody from your race you could claim .

maybe you’d look up to someone , but that person wasn’t from your race or ethnicity .

my paternal grandfather was the only black man in new iberia , louisiana who could read . everyone brought him their contracts , letters or whatever else required a written response .

my father , like his father and like socrates , was first and foremost an educator . to me , my dad was a ‘black socrates . ’

the terrible truth is that killing someone doesn’t automatically make you a monster ; most murders are simply seconds of madness in an otherwise ordinary life .

challenge yourself to jump into a new series this summer . three of our favorites are on sale right now—time to give your ereader a workout !

the second girl by david swinson is $ 1 . 99 : google play | ibooks | kindle | kobo | nook

say you’re sorry by michael robotham is $ 2 . 99 : google play | ibooks | kindle | kobo | nook

a drop of the hard stuff by lawrence block is $ 2 . 99 : google play | ibooks | kindle | kobo | nook

an important book that’s near and dear to our hearts is on sale for $ 2 . 99 today—download a copy from your ebook retailer of choice : google play | ibooks | kindle | kobo | nook

“this one kept me up at night and changed the way i saw the world once i was finished . ” —ann patchett , time

the beginning of a new series by william shaw—hop aboard with a free early copy of the birdwatcher ! you can request your copy from goodreads .

david swinson put together a playlist for his new mystery , crime song , and here is your daily excuse to listen to the fall at work .

introducing frank marr—a good detective with a bad habit . if you haven’t yet read david swinson’s mystery , which the new york times called one of the best of 2016 , you can purchase the ebook this week for $ 1 . 99 . act now ! discount ends sunday : google play | ibooks | kindle | kobo | nook

a cutting - edge thriller about one man’s quest to discover horror lurking at the top of the world . enter to win a free advance copy of the white road from goodreads !

success to me is finding the culture and the environment that will support risk - taking . the creative fire is fed by risk , and the commercial marketplace often lulls you into complacency .

malcolm mackay portrays organized crime as so enamored of hierarchy and meetings that one can almost imagine the gang going off on a retreat , with pastries and facilitators .

this quote ( within a rave review of every night i dream of hell ) amuses me .

inspired by the work of saul bass , art goodman and dave nagata . hitchcock typeface by matt terich . tumblr theme by matthew buchanan .

please take a moment to review hachette book group’s updated privacy policy : read the updated policy here .

new york , ny — june 29 , 2018 the seventh star , the laconic , unflinching memoir by swifty mcvay , detroit - based rapper and member of the legendary hip - hop group d12 , will be published by hachette audio in original audiobook format on july 10 , 2018 . read by the author himself , listeners will embark on a journey that takes them [ … ]

html public " - / / w3c / / dtd xhtml + rdfa 1 . 0 / / en " " urltoken "

this content was extracted from wikipedia and is licensed under the creative commons attribution - sharealike 3 . 0 unported license

michael wood is girding himself for a summer teaching in vermont . on empson is out now .

we see similar shots of the los angeles area several times in the film . this is the mythical metropolis , the last american city going west , the place where all dreams end , the place made only of dreams , and the director wants us to join him in living there for a couple of hours . it’s not an easy place to be , partly because even dreams are not made of dreams , only of mixed wreckage from the waking day , and partly because this is david lynch’s particular choice of wreckage , full of ambition , danger and ambiguity . the city is ‘mysterious and apparitional’ , as dennis lim says in his excellent new book on lynch , and never more so than in this film . [ * ]

i am calling the first part of the film a dream because that is what most people call it , and because it has elements that clearly belong to the fantasy of the character in the second part . but it doesn’t have to be a dream : it could just be a tale she tells herself , or a long memory , or a memory tipping over into fiction . all we can really say is that the levels of proposed reality within the film clearly shift ; and that naomi watts , who used to be betty , becomes diane . diane , to put it at its simplest , has invented betty .

diane is an actress who has lost her love , a woman , to a director ( and perhaps to another actress on the side , unless this is diane’s fantasy ) . in the intervals of seeing her affair go wrong and plotting vengeance , diane creates the story of the movie’s first two hours . betty , a girl from deep river , ontario , has won a jitterbugging competition – that’s what all those antique dancers and silhouettes were about at the start of the film – and has come to hollywood to try her luck . her luck includes finding the amnesiac survivor of an assassination attempt in the apartment she is borrowing , and the two women go on a quest to find the survivor’s lost past . the quest turns up various memories and locations , including a magical theatre and a corpse that may belong to diane herself – a figure of fear that has crept from one story into another .

there is quite a bit of aimless hocus - pocus in the movie involving blue keys and a mysterious blue box . this is presumably meant to remind us that lynch is a magician and can do what he likes with his film , in or out of the metaphysical cabaret . the plotline about the director - character within the movie and his difficulties with the mafia , who have a strong interest in a particular bit of casting , feels a bit thin too . but the most extraneous - looking scenes in the whole work , apart from being beautifully , slowly , acted and shot , are also the least extraneous to the film’s preoccupations .

two men sit in a diner on sunset boulevard . one looks perturbed and mentions a dream he has had – twice . he describes the dream . it has him sitting in this same diner , his companion also in the room but over by the till , and the dreamer perceives ( he says he sees through a wall ) a ghastly - looking old man behind the building controlling events , terrifying everyone . the dreamer’s waking hope is never to see this man’s face ‘ever , outside of a dream’ . this problem would seem to be easily solvable and the two men leave the diner to encounter the nothing they expect to find . as they turn the last corner to reach the back wall they see a ghastly - looking old man , all long hair , blackened face and stereotyped horror , a sort of movie caveman . both dreams and reality can be a little predictable .

towards the end of the film diane sits in the same diner , in the process of paying a hitman to kill her ex - friend camilla . the man who told the dream earlier stands outside , looking austere , saying nothing . behind the diner the ghastly - looking man sits by a fire , apparently using some kind of voodoo to manufacture the tiny figures who are going to creep under diane’s door and drive her to suicide .

the structure of thought here resembles that of david cronenberg’s the brood ( 1979 , also newly released by criterion ) , where a therapy that deals in working out one’s rage creates a set of small creatures who go off and beat in the heads of people you don’t like . the point , i take it , is not that dreams always come true , or that fact is more fantastic than fiction , but that nothing in the mind is permanently guaranteed not to find a partner or echo in reality . ‘whatever is thinkable is also possible , ’ as wittgenstein said , and just because you dream of a scary old man in the neighbourhood doesn’t mean there are no scary old men in the neighbourhood .

[ * ] david lynch : the man from another place by dennis lim ( houghton mifflin harcourt , 192 pp . , $ 20 , march , 978 0 544 34375 7 ) .

some promotions may be combined ; others are not eligible to be combined with other offers . for details , please see the terms & conditions associated with these promotions .

sir winston churchill was a soldier , journalist , writer , nobel prize winner and , above all , a leader . conservative then liberal then conservative again , his political instincts won him a sustained career at the summit of british government , while his resolve and politics of personality made him broadly regarded as one of the greatest wartime leaders of the 20th century .

with his early radicalism , bold decisions regarding the gold standard and iron curtain analysis , churchill was , for many , a highly controversial figure . for others , he was britain’s finest prime minister . from his career as a young army officer – serving in british india , the sudan , and the second boer war , in which he won fame as a war correspondent – to his later pursuits as a historian , a writer , and an artist , ‘churchill : history in an hour’ is the perfect guide to the colorful , long and varied life of a historic titan .

# apollo11 : when men walked on the moon : the incredible mission of apollo 11 ( hashta . . .

sponsored products are advertisements for products sold by merchants on amazon . when you click on a sponsored product ad , you will be taken to an amazon detail page where you can learn more about the product and purchase it .

this shopping feature will continue to load items . in order to navigate out of this carousel please use your heading shortcut key to navigate to the next or previous heading .

an excellent and well balanced account of churchill ' s extremely busy life . this book is an ideal introduction to the subject , covering all of the ground in an accessible manner . it also manages to pick it ' s way through some of the controversies and myths of churchill ' s life , in an insightful and objective manner . the less well known aspects of his extraordicnary career are given due weight in what is ( another ) great read .

thank you for an easy , pleasant transaction . will buy again . excellent seller .

excellent book delivering a comprehensive study of churchill ' s life in one hour . more about his personal life than political and allows the real man behind the power to shine through .

great overview of a great man . also makes one compare him to present day politicians and regretfully concluding churchill was a statesman whereas now we only have politicians .

a very interesting read . concise with a lot of facts concerning this iconic person .

enjoyed this brief insight into the life of churchill . covered all the major events he lived through as well as influenced .

i suggest reading this and then exploring churchill by roy jenkins to get a sound idea of the man and the legacy .

if you need help or have a question for customer service , contact us .

we don ' t know when or if this item will be back in stock .

instantly receive a £10 urltoken gift card if you’re approved for the amazon platinum mastercard with instant spend . representative 21 . 9 % apr ( variable ) .

credit offered by newday ltd , over 18s only , subject to status . terms apply .

2009 hbg announced the launch of a formal environmental policy in november , one of the strongest and most progressive of any u . s . book publisher , committing the company to dramatically increasing the use of recycled fiber , ending the use of paper that may impact endangered forests , reducing greenhouse gas emissions , and a wide range of other initiatives .

hbg launches the hachette speakers bureau ( hsb ) , in association with greater talent network , a leader in the lecture industry ( urltoken ) .

2010 hbg adopts the agency model of sales : as of april 1 , 2010 , all ebook titles published by hbg will be sold directly to consumers by means of third party agents .

grand central publishing announces the creation of a new practical nonfiction imprint , grand central life & style , combining and closing two existing imprints , springboard press and wellness central , which will launch in the fall 2010 season . orbit announces a digital - only short fiction publishing program launching later this year .

orbit announces the creation of redhook , a new commercial fiction imprint which will publish across a range of genres , and in all formats ( including digital - only ) launching in april 2013 .

2013 michael pietsch becomes ceo of hachette book group ; david young becomes chairman ( stepping down as ceo , and moving to other roles within hachette livre ) .

hbg purchases adult trade publishing imprint , hyperion , from the walt disney company ( deal closes 7 / 16 / 2013 ) .

2015 james patterson launches a new children ' s book imprint , jimmy patterson , with the goal of turning kids into lifelong readers . the new jimmy imprint is housed under the little , brown and company division .

grand central life & style , an imprint of grand central publishing , announces the creation of a new imprint called goop press , an extension of the lifestyle brand founded by gwyneth paltrow , to begin publishing in april 2016 .

james patterson launches the bookshots imprint , with the goal of publishing short reads that can be read on on any device . the imprint is housed under the little , brown and company division .

the yen press imprint becomes a joint publishing venture , with japanese company kadokawa owning a majority share .

my research interests concern ireland since the famine , and the history of political thought since the french revolution . i ' m particularly keen to supervise research in the fields of twentieth - century irish history , and in the history of socialism .

bourgeois liberty and the politics of fear : from absolutism to neo - conservatism ( oup oxford , october 2012 ) .

in 1842 heinrich heine , the german poet , wrote that the bourgeoisie , ' obsessed by a nightmare apprehension of disaster ' and ' an instinctive dread of communism ' , were driven against their better instincts into tolerating absolutist government . theirs was a ' politics motivated by fear ' . over the next 150 years , the middle classes were repeatedly accused of betraying liberty for fear of ' red revolution ' . the failure of the revolutions of 1848 , conservative nationalism from the 1860s , fascist victories in the first half of the twentieth - century , and repression of national liberation movements during the cold war - these fateful disasters were all explained by the bourgeoisie ' s fear of the masses . for their part , conservatives insisted that demagogues and fanatics exploited the desperation of the poor to subvert liberal revolutions , leading to anarchy and tyranny . only evolutionary reform was enduring .

from the 1970s , however , liberal revolution revived on an unprecedented scale . with the collapse of communism , bourgeois liberty once again became a crusading , force , but now on a global scale . in the twenty - first century , the armed forces of the united states , britain , and nato became instruments of ' regime change ' , seeking to destroy dictatorship and build free - market democracies . president george w . bush called the invasion of iraq in 2003 a ' watershed event in the global democratic revolution ' . this was an extraordinary turn - around , with the middle classes now hailed as the truly universal class which , in emancipating itself , emancipates all society . the debacle in iraq , and the great recession from 2008 , revealed all too clearly that hubris still invited nemesis .

bourgeois liberty and the politics of fear examines this remarkable story , and the fierce debates it occasioned . it takes in a span from the seventeenth century to the twenty - first , covering a wide range of countries and thinkers . broad in its scope , it presents a clear set of arguments that shed new light on the creation of our modern world .

in irish history , i look at the ' national question ' ( almost inevitably ) . this has included studies of the press , of ulster unionism , and of the provisional ira . i have written a biography of terence o ' neill , prime minister of northern ireland from 1963 to 1969 . i am also preparing a new large - scale project on irish history based upon a fresh plunge into archives new to me .

in terms of theory , i have written on the salience of class as an interpretative framework for modern history . somewhat unfashionably , i have argued for a rehabilitation of the marxist framework , whilst insisting that it has not been well understood hitherto . my work on the history of political thought focuses less upon high theory , more upon its mid - range grappling with the exigencies of societal conflict . this has produced a recent book covering certain lines of debate from absolutism to neo - conservatism .

& quot ; bibliography of the major writings of roy foster to 2014 & quot ; - - pages 283 - 289 .

this book brings together some of today’s most exciting scholars of irish history to chart the pivotal events in the history of modern ireland while providing fresh perspectives on topics ranging from colonialism and nationalism to . . .

the " hilarious and poignant " story of one chronically anxious woman ' s year - long quest to become braver by seeking out the kinds of experiences she ' s spent her life avoiding . ( cheryl strayed )

a sharp new collection from the " hilarious " ( washington post ) humorist who draws comparisons to douglas adams ( the new york times ) , james thurber , and p . g . wodehouse ( the guardian ) .

set in modern shanghai , a debut by a chinese - american writer about a prodigal son whose unexpected return forces his newly wealthy family to confront painful secrets and unfulfilled promises .

a mesmerizing psychological thriller about how a secret can bind two friends together forever . . . or tear them apart .

a memoir that braids together the evolution of one of america ' s most iconic branding campaigns with the stirring story of the women who lived behind its façade - - told by the heiress to its fractured fortune .

from new york times bestselling author elin hilderbrand , comes a novel about the many ways family can fill our lives with love . . . if they don ' t kill us first .

“frankly , i have no use for messrs . little and brown . . . it’s the ‘and company’ i’m fond of . ” —ogden nash

to this day , little , brown remains committed to publishing fiction of the highest quality and nonfiction of lasting significance . the company’s bestselling novelists include j . d . salinger , herman wouk , james patterson , anita shreve , michael connelly , alice sebold , janet fitch , pete hamill , emma donoghue , david foster wallace , kate atkinson , stephenie meyer and elin hilderbrand . in its early days , little , brown published little women and little men by louisa may alcott , man without a country by edward everett hale , and the poetry of emily dickinson . over the years it has brought out important novels by norman mailer , thomas pynchon , gore vidal , evelyn waugh , john fowles , thomas berger , erich maria remarque , james carroll , richard yates , john p . marquand , ellery queen , ngaio marsh , c . s . forester , john barth , and rick moody .