Background: Melanoma is a cancer of the skin with potential to spread to other organs and is responsible for most deaths due to skin cancer.,It is imperative to identify immune biomarkers for early melanoma diagnosis and treatment.,Results: 63 immune-related genes of the total 1039 unique IRGs retrieved were associated with overall survival of melanoma.,A multi-IRGs classifier constructed using eight IRGs showed a powerful predictive ability.,The classifier had better predictive power compared with the current clinical data.,GSEA analysis showed multiple signaling differences between high and low risk score group.,Furthermore, biomarker was associated with multiple immune cells and immune infiltration in tumor microenvironment.,Conclusions: The immune-related genes prognosis biomarker is an effective potential prognostic classifier in the immunotherapies and surveillance of melanoma.,Methods: Melanoma samples of genes were retrieved from TCGA and GEO databases while the immune-related genes (IRGs) were retrieved from the ImmPort database.,WGCNA, Cox regression analysis and LASSO analysis were used to classify melanoma prognosis.,ESTIMATE and CIBERSORT algorithms were used to explore the relationship between risk score and tumor immune microenvironment.,GSEA analysis was performed to explore the biological signaling pathway.
We have attempted to characterize the changes occurring on the host side during the progression of human melanoma.,To investigate the role of tumor microenvironment, we set up such an animal model, which was able to isolate the host related factors playing central role in metastasis formation.,One of these ‘factors’, CCL12, was consequently selected and its behavior was examined alongside its human homologue (CCL8).,In our animal model, metastasis forming primary melanoma in the host exhibited increased level of CCL12 mRNA expression.,In clinical samples, when examining the tumor and the host together, the cumulative (tumor and host) CCL8 expression was lower in the group in which human primary melanoma formed lung metastasis compared to non-metastatic primary tumors.,We could not detect significant difference in CCL8 receptor (CCR1) expression between the two groups.,Increased migration of the examined tumor cell lines was observed when CCL8 was applied as a chemoattractant.,The tumor cells and their interactions can be influenced the expression of CCL8 by dermal fibroblasts, as a significant change in the metastatic microenvironment.,Furthermore, we examined changes in miRNA profile resulted by CCL8 and miR146a appears to be a promising prognostic marker for following this process.