Students investigate the rates of enzyme catalyzed reactions by observing the uncatalyzed decomposition of hydrogen peroxide by catalase. The effect of heating the catalase is also determined. Titration, a key laboratory method, is used to determine the rate of reaction—an important scientific concept.
Support stands, clamps, pipet bulbs, test tubes, boiling water bath and a potato are required and available separately.
For 3 groups
Materials Included in Kit: Catalase, 0.5 g Hydrogen peroxide, 3%, 473 mL Potassium permanganate solution, 2%, 250 mL Sulfuric acid solution, 2 M, 250 mL Bottle, Amber, HDPE, with cap, 16 oz, 2 Pipet, Beral-type, wide stem, 6 Pipet, serological, 2 mL, 3 Pipet, serological, 10 mL, 6 Plastic beakers, 110 mL, 24 Replacement stopcock, 3 Scalpel, disposable, size 22 Syringe, 12 mL, 6
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Correlation to Next Generation Science Standards (NGSS)†
Science & Engineering Practices
Developing and using models Planning and carrying out investigations Analyzing and interpreting data Using mathematics and computational thinking Obtaining, evaluation, and communicating information
Disciplinary Core Ideas
MS-PS2.B: Types of Interactions HS-PS1.A: Structure and Properties of Matter HS-LS1.A: Structure and Function
Patterns Cause and effect Structure and function Stability and change
HS-PS1-1. Use the periodic table as a model to predict the relative properties of elements based on the patterns of electrons in the outermost energy level of atoms. HS-PS1-5. Apply scientific principles and evidence to provide an explanation about the effects of changing the temperature or concentration of the reacting particles on the rate at which a reaction occurs. HS-PS2-6. Communicate scientific and technical information about why the molecular-level structure is important in the functioning of designed materials. HS-LS1-1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells. HS-LS1-3. Plan and conduct an investigation to provide evidence that feedback mechanisms maintain homeostasis.