Source: SEAMEO BIOTROP's Research Grant | 2015
Until now mistletow hesitation at teak plantation in Jawa in Perhutani region has extended in a widespread scale of areal extents and distribution in teak plots in Forest authority units (FAU/KPH) and Clonal seed garden (CSO), especially CSO Padangan. To support early detection system for controlling teak mistletoe is needed supporting basic research among others ‘Diversity analysis and DNA barcode as reference for applied research. Purpose this research is DNA Analysis for parasitic plant (mistletoe) diversity on a stand of teak, and apply DNA Barcode technical with nukleotida difference analysis and genetic distance to determine mistletoe species hesitate teak stand. Research procedure for diversity DNA covering take and treat sample, extraction and testing DNA quality, process PCR-RAPD, data analysis usage POPGENE versi 32 dan NTSys. For DNA barcode covering identify teak mistletoe species based of morphology, take leaves samples for measuring leaf morphologi character and DNA Barcode covering PCR with core marker (matK, rbcL), sequencing DNA, documentation, DNA Barcode analysis and filogenetik. By multivariate analysis (PCA, MCA) can distinguish two teak mistletoe Dendrophthoe pentandra and M. tetragonus. Reinforced result significant different test for every leaf morphology variable those mistletoe showed some variable different significant i.e petiole length, leaf width, sum secondary vein, sum leaf, leaf base form, aspect ratio, form factor, perimeter ratio. Besides, primer matK dan rbcL that used at DNA Barcode can distinguish based filogenetic among D. pentandra, M. tetragonus and Viscum articulatum so two core primer were recommended usage for DNA Barcode teak mistletoe. As for deteminating genetic diversity of mistletoe teak, exactly marker RAPD (OPP 9, OPBH 16, OPBH 20), can amplification success to teak mistletoe of D. pentandra.