SDS in PCR?
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Tue Jan 4 10:02:28 EST 2000
In article <84sm4a$u4j$1 at clematis.singnet.com.sg>, Chong Wai Yin( Zhang
Weixian) <wychong at singnet.com.sg> writes
>Hi will the addition of SDS to PCR mixture affect the process of PCR (eg.
For Taq polymerase
Concn (presume final) Activity
However addition of 0.5% each Tween20 + NP40 reverses the inhibitory
effect of 0.01% SDS.
The enzyme is also happy in 2M Urea, 10% ethanol, <5% DMF and <10%
All from PCR Technology, Principles and Applications for DNA
Amplification, Ed. Henry Erlich, Stockton Press. Table 1, page 20 in the
chapter written by David Gelfand. Of course Promega may dispute this
information. I merely pass it on without too much comment.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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