SAS cells grown on cover slides were fixed with 3.7% paraformaldehyde for 15 min and permeabilized with 0.2% Triton X-100 in PBS (0.58M Na2HPO4, 0.17 M Na H2PO4, 0.68 M NaCl, pH=7.4)(PBST) for 5 min. After washing the cells with PBST three times, the cells were blocked for 30 min in PBS containing 1% bovine serum albumin. Immunostaining was performed by incubation with anti-ANX-1 monoclonal antibody (1:800 dilution) for 2h. After washing the cells with PBST three times, the cells were incubated with TRITC-conjugated rabbit anti-(mouse IgG) Ig for 1 h. Cover slides were washed with PBST, mounted, and examined using a Leica TCS SP2 Confocal microscope (Leica Microsystems, Wetzlar GmBH, Germany).