Samples will undergo bisulfite-conversion, and unmethylated cytosines will be deaminated, forming uracils. Methylated cytosines will remain unchanged. The location of the methylated cytosine of the gene sequence and the amount of methylated cytosine will be identified and quantitated by Pyrosequencing using established techniques. Results will be expressed as % methylation in the promoter region of IL-6 and NF-ĸB.