Lab analysis: blood samples for biochemical determinations will be obtained in Vacutainer tubes of 10 ml without additives. Blood will be clotted at room temperature for 30 minutes and centrifuged at 2500 rpm for 15 minutes. After separating the serum from the clot (about 4-5 ml), will be divided into 1 aliquot of 1.5 ml (for the study of lipids) and 5-7 aliquots of 0.5 ml aliquots which will be frozen at -20 °C until the biochemical analysis are carried out.