The EDTA sample would then be centrifuged at 300 rpm for 10 minutes to separate the plasma, which will be kept in properly labelled 2ml Eppendorf tubes. The samples would be stored at -20°C at APC 4A Hematology Lab. Processing would be done in batches every 2nd day by chemiluminescence assay using ADIVA Centaur Ferritin assay.