A biopsy of the quadriceps will be obtained with a Bergstrom needle at the beginning of the study, 4 h (immediately before the insulin infusion) and at the end of the 4 h insulin infusion. We will use the A-V balance technique to address the relation between tissue fatty acid and TG metabolism and the insulin responsiveness of glucose uptake and myofibrillar and mitochondrial protein synthesis and net protein balance.