A target total of > 10,000 IE/kg recipient body weight will be infused via a percutaneous transhepatic catheter inserted into the portal vein (see Section 1.4.2). This will in all likelihood require more than one islet infusion to achieve this goal. The first infusion must contain at least 5,000 IE/kg recipient body weight. In the event that a subject does not achieve insulin-independence with normoglycemia after two fresh islet infusions, a third transplant may be considered as described in Section 5.1.