Sample size There is no data regarding how different dietary status (fed versus fasted) affects the acute responses to exercise on overweight males. However, a previous study using a similar meal showed that feeding had an enormous effect on the use of lipid during exercise - and thus indicates that the role of adipose tissue during exercise would be potentially very different. In order to see an effect on lipid oxidation during exercise with 95% power and 5% alpha, we would require 6-8 participants. To ensure that these results are generalizable and also to account for greater variability in other outcome, we recruited a total 10 participants from the study.