1.3 Combined HBV vaccine and NA therapy in animal study A Woodchuck animal study suggest entecavir therapy followed by Woodchuck surface (WHs) and core (WHc) DNA vaccination 7 weeks later may induce WHc and WHs specific T cell responses. Two of the 6 animals clear Woodchuck surface antigen (WHBsAg) and product anti-WHBs (48). Similar results can be found in HBV transgenic mice model. Hepatitis B surface (HBs) and hepatitis B core (HBc) specific cellular and humoral response was identified after HBc and HBs protein vaccine therapy (49). In a mouse model with sustained HBV viremia after infection with a recombinant adeno-associated virus carrying a replicable HBV genome. Immunization with the conventional HBV vaccine in the presence of aluminum adjuvant failed to elicit an immune response,but vaccination of mice with HBsAg/CpG induced strong antibody production and T-cell responses(50)