Donor-specific anti-HLA antibodies(class I,II) and MHC complex class I - related chain A (MICA) antibodies will be measured (before heart transplant, 1 month, 6 months and 12 months after heart transplant). Evaluation of anti-HLA antibodies levels (titers) in the serum of a patient is performed by fluorescence intensity of formed complexes. Double laser cytometer Luminex 100 IS 2.3 is used for fluorescence reading. Based on comparison of negative and positive controls, cut-off is calculated.