Pre-treatment with PD098059, Ly294002 and SB203580 The cells were plated overnight in complete medium and then washed. The cells cultured in serum-free DMEM were pre-treated with 50M PD098059 (inhibitor of ERK), 30 M Ly294002( inhibitor of PI-3 kinase) and 20 M SB203580(inhibitor of p38) for 3 hrs respectively, and then they were treated with 40ng HGF for 3hrs. The cells were checked by immunohistochemistry and Western blot with ANXA1 antibody.