(iii) Biochemical analysis will be performed in the laboratory of clinical pathology at the HSM. Blood samples will be collected after overnight feasting (10-12 hours) in the presence of one of the parents/ caregivers, and after a local application of a topical anesthesia patch (EMLA). Blood glucose levels will be determined using hexokinase method and insulin will be assessed using a chemiluminescence immunoassay technique. Insulin sensitivity will be derived from the homeostasis model assessment (HOMA) method. Total cholesterol, triglycerides, and high density lipoprotein cholesterol will be determined using enzymatic, GPO-trinder, and direct methods, respectively. Plasma liver enzymes (AST, ALT, and GGT) will be assessed with modified IFCC method. C-reactive protein will be determined using a turbidimetric immunoassay (Siemens, ADVIA 2400, Newark, DE, USA).