Plasma lipids, glucose, glycosylated hemoglobin, insulin and C-peptide: measurements of total cholesterol and triglycerides will be performed using automated trading methods (enzyme colorimetry, Roche Diagnostics) adapted to the automated autoanalyzer Hitachi 911. The determination of apolipoproteins AI, B and C-III, lipoprotein (a) will be held mediate commercial methods (immunoturbidimetric assay, Roche Diagnostics and Wako Chemicals) adapted to the automated autoanalyzer Hitachi 911. The determination of cholesterol lipoprotein fractions (Lipid, ELIP) (VLDL, LDL, HDL) will be performed by the following methods: VLDL separated by ultracentrifugation of flotation, cholesterol HDL determined by a direct method (Roche Diagnostics) and cholesterol LDL determined by difference in total cholesterol and VLDL and HDL cholesterol fractions. Glucose: by a commercial method (enzyme colorimetry, Roche Diagnostics) adapted to the automated autoanalyzer Hitachi 747. Serum insulin concentration will be determined by non-competitive chemiluminescent enzyme immunoassay (Immulite 2000TM, Diagnostic Products Corp., Los Angeles, CA, USA). The serum concentration of C-peptide is determined by non-competitive chemiluminescent enzyme immunoassay (Immulite 2000TM, Diagnostic Products Corp., Los Angeles, CA, USA).