3. To perform molecular analysis including polymerase chain reaction (PCR) amplification, flow cytometry, immunohistochemistry, and gene analysis of material from NBECs, tumors, adjacent and normal lung and blood for evaluation such as mutations in the K-ras and p53 protooncogenes, as well as other candidate genes and pathways such as those involved in epithelial-mesynchymal transition. In addition, we will look for mutations and alterations of expression of Fas, Fas ligand, and FADD, three molecules which mediate programmed cell death and have recently been shown to be expressed on multiple tumor cells including lung cancer.