Lastly we will examine the exact timing of biomarker alteration using a timed animal model of hypoxic-ischaemic injury (HI). In clinical studies hypoxic-ischaemic injury is unpredictable and its timing is often impossible to estimate. Dr Tracey Bjorkmann in Queen's University, Brisbane has developed a piglet model of HI which produces HI induces seizures and a level of injury equating to moderate to severe HIE (12). She has agreed to collaborate with our programme and will provide piglet samples of serum taken at 0,1,2 and 12 hours following HI. These samples will be invaluable in examining the progression of biomarker derangement and will help us to time neonatal brain injury after delivery.