Following an overnight fast, participants will be instructed to drink 500 mL of water, but to delay all morning-time medication (apart from metformin) until conclusion of the examination day. Subjects will arrive at the Clinical Research Unit (CRU) at 07.30 ante meridiem (a.m.). After taking subjects history, current weight and blood pressure, intravenous catheters will be placed in both forearms after which blood and urine will be collected. Then, subjects will assume a semi-recumbent position throughout the duration of the visit and after a resting period, an automatic blood pressure measurement is performed. Thereupon the combined renal and clamp protocol will commence. During the first equilibration period, body fat content assessment and the cardiovascular tests are carried out.