3. In addition to the biochemical data generated by our collaborators at MUSC and the University of Washington, a DCCT/EDIC Genetics Project has been initiated. (A proposal for continuation of the genetics project will be submitted separately). Our collaborators at the Hospital for Sick Children/University of Toronto have received DNA from virtually all the participants and from 2918 first degree relatives, representing 87% of the relatives who were not available and did not refuse to provide their DNA outright (25% of all relatives). Genetic analyses for genes influencing CVD are being conducted by 3 approaches: population based association in the DCCT/EDIC probands with candidate genes; "trios" analysis with probands and their parents and siblings; family based associations with multiplex families in whom relatives have also been previously clinically phenotyped for complications.