Data collected will be analysed using the same method as previous study. Statistical software SAS version 6.12 (SAS Institute, Cary, NC, USA) will be used to perform the analysis. Parametric data will be presented as mean (SD) and non parametric data will be presented as median (range) or actual count or percentages. These results will then be compared with those from the previous study. Differences between groups will be compared using Mann-Whitney U or Kruskal-Wallis test, as appropriate.