EXPECTATIONS: The combined analysis of insulin clamp and mixed meal test (MMT) data will allow to build a comprehensive model of the glucose insulin system during a MMT in each subject, which will thereby work as an in silico virtual patient, according to a well established methodology developed in our laboratory. The collection of virtual patients with type 1 diabetes undergoing a MMT will form the in silico biobank derived from this study. Virtual patients will be used to carry out MCA to compute CCs. Furthermore, the incretin hormone, glucagon and substrate response to a mixed meal will be quantified, allowing the putative identification of further modifiers of the glucose-insulin system. This database will be instrumental in devising a control algorithm able to guarantee a normal glucose regulation during a mixed meal in patients with type 1 diabetes with CGM on insulin pump therapy.