Group B streptococci (GBS) colonizes the vagina and rectum in 10-30% of pregnant women. In the newborn, GBS is a leading cause of neonatal sepsis and a major cause of pneumonia and meningitis. Intrapartum penicillin G prophylaxis aims to prevent early-onset group B streptococci (GBS) sepsis by interrupting vertical transmission. According to the Center of Disease Control and Prevention (CDC) guideline in 2002, a minimum of 4 hours of intrapartum antibiotics prophylaxis is generally recommended to prevent early-onset group B Streptococcous (GBS) because less than 4 hours may not achieve adequate levels drugs. Even with the most valiant of efforts, there will frequently be GBS-positive mothers who arrive at the labor floor and deliver in fewer than 4 hours, especially in multiparous mothers. Preliminary studies as well as data from a large health maintenance organization demonstrated the 40-50%of GBS-colonized women do not receive antibiotics at least 4 hours before delivery due the rapidity of their labors. Newborns of all GBS-positive women who present to labor units and deliver before receiving 4 hours of intrapartum antibiotic prophylaxis are deemed as "at risk" and recommended to undergo blood cultures, complete blood count, and 48 hours of observation. This problem is not easily resolved by antibiotic treatment of the pathogen; thus, such tranditional approaches to patient management need to be re-evaluated.