The preparation of the key reagent (anti-CD19-BB-zeta mRNA) is finalized in the Tissue Engineering & Cell Therapy (TECT) Laboratory at NUH, where the GMP-compliant MaxCyte electroporator is located. The feasibility of large-scale expansion of NK cells has been demonstrated (Shimasaki et al. Cytotherapy 2012), and the feasibility of large-scale electroporation validated in the TECT laboratory.