Single pulse TMS-Resting motor threshold (RMT) measurement Singe pulse TMS was delivered using Magstim Bistim² stimulator via a double cone coil. The double cone coil was oriented to induce a posterior-anterior current flow in cortex. The coil was placed on the cap with the intersection of the two embedded coils located over the marked point. The coil position was maintained manually by an assistant. Surface electromyography (EMG) electrodes are attached to the TA for EMG recording. The "hot spot" of the motor evoked potential (MEP) from TA is identified first from one of the marked points for both left and right side. This spot was marked on the elastic cap and used for all recordings on that side. The lowest intensity needed to elicit a MEP response of at least 50µV amplitude and that has been elicited in 50% of 8 successive trials will be recorded as RMT.