3. Development of biomarkers for treatment of patients with type 2 diabetes by amlexanox. We will evaluate the phosphorylation of proteins known to be targets of IKKe and TBK1 in blood, and adipose tissue samples. We will use phospho-specific antibodies to evaluate the regulation of targets that play a role in energy metabolism, and also in feedback inhibition of NFkB. These studies will pave the way for future evaluations probing the metabolic relevance of IKKe and TBK1 in humans using the same agent or more potent ones to be discovered or designed.