Blood Collection: Blood samples will be collected by venipuncture of an arm vein by a trained phlebotomist as with previous studies conducted in our lab. Briefly, participants will be sitting comfortably in a chair with an armrest for the specimen collection. In addition, subjects will be thoroughly briefed regarding the blood draws, and blankets, pillows, food and drink will be available in the event a subject reacts negatively. Standard sterile blood handling techniques will be used including disposable medical examination gloves, alcohol swabs, and needles. All blood contaminated materials will be disposed of in a hazardous material labeled disposal container, promptly after analysis. The specimens will be collected in EDTA-treated tubes, undergo treatment following ELISA kit protocols, centrifuged, and stored in a freezer at -70°C in the Exercise Nutrition Research Lab until hormone analysis in the same laboratory. The frozen specimens will remain stable for approximately 1 month. Therefore, the blood analyses will be completed within that time frame, after which they will be disposed of appropriately. The samples will be controlled and cared for by the principal investigator Dr. Peter Lemon and Kolten Abbott.