Immunocytochemistry(IHC) Formalin-fixed, paraffin-embedded tumor samples were assessed for annexin A1 expression.The IHC procedures was followed as the reference18, The monoclonal primary antibody anti-Annexin AI(1:600 dilution), directed against the the N-terminus of annexin 1 were obtained from a signal source (BD Biosciences). Protein translocation as determined by two pathologists (Jeng and Lin) using immunohistochemistry was scored as negative (score 0), positive (score 1) and cytoplasmic protein expression was scored as negative (score 0), weak (score 1), moderate (score 2), or strong (score 3) using a system that has been previously validated.