A successful urine specimen obtained using the bladder stimulation technique will be defined by the collection of at least 1 mL of urine within 300 seconds of initiating the bladder stimulation maneuvers. Laboratory definitions of a positive urinalysis and urine culture are defined below based on definitions published by the American Academy of Pediatrics and previous authors investigating the bladder stimulation technique. As per Labrosse et al, poor oral intake will be defined as <25% of regular fluid intake during the feeding period prior to the bladder stimulation technique, based on parental assessment. Finally, the cost of the procedures (invasive versus non-invasive) will be based on the cost of material required for a clean catch urine specimen versus the cost of material required for bladder catheterization.