Development of pulse oximeters requires calibration and evaluation for accuracy. There is no acceptable surrogate calibration tool for pulse oximeters. To quote the current International Organization for Standardization (ISO) Pulse Oximetry standard document: "There is today no accepted method of verifying the correct calibration of a pulse oximeter probe/pulse oximeter monitor combination other than testing on human beings. This is due to the complexity of the optical intricacies of the interaction of light and human tissue upon which pulse oximetry depends".