There is no standard tool for calibrating pulse oximeters. According to the ISO pulse oximetry standard document, "there is today no accepted method of verifying the correct calibration of a pulse oximeter probe/pulse oximeter monitor combination other than testing on human beings. This is due to the complexity of the optical intricacies of the interaction of light and human tissue upon which pulse oximetry depends". In-vitro calibration device prototypes using whole blood have been suggested in various research projects, but these systems are, to-date, not commercially available and very expensive to build and operate. They have not been accepted as standard tools for calibration because of their lack of simulating tissue interaction with pulse oximeter light. It is therefore necessary to calibrate pulse oximeters on human subjects.