 La dose  di melfalan deve essere calcolata in accordo al metodo litro-volume di Wieberdink (Wieberdink J, Benckhuysen C, Braat RP, van Slooten EA, Olthius GAA.  Dos imetry in isolation perfusion of the limbs by assessments of perfused tissue volume and grading of toxic tissue reactions.  E ur J  Cancer Clin Oncol  1982;  18: 905-910.), fino ad una dose massima di 150 mg. 