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Gayle Classic | Classic Black & Spot
Classic style meets modern design in the gorgeous Gayle one piece. Her carefully constructed cups will flatter both the bosom-ly blessed & the smaller chested Siren. Wide halter straps begin at the side and ensure you won't be nursing a sore neck at the end of a day at the beach. A ruched torso and soft overlay will flatter tummies and a delicate vintage finish.
- Shelf Bra
- Molded Cups
- Wide halter straps
- Shapewear lining
Try our swimsuits in the comfort of your home with our Try@Home Service. See the Try@Home tab below for more information.
Not sure of your size? Please check the sizing guide tab below.
Will I have to pay extra for Try@Home shipping?
Because we want everyone to experience the benefits of try before you buy, we've made sure shipping for Try@Home multiple items is only $14.95.
You are sold out of the style/size that I want. When will it be back in stock?
Each Sirens release is unique, to be sure you're getting a special and exclusive style we only do a limited run of each style in each size. Once a style sells out we often don't restock it – so if there is something you love be sure to get in quick! Keep up to date with season releases by subscribing at the bottom of our page.
Do you do make to order/custom garments?
We are unable to make special order garments. However, we'd love to help you to pick a style from the existing range that you will adore! You can email us on email@example.com to see what styles may be best suited to your body shape. Alternatively, you can read about Which Style Suits your Body Shape in our buying guide.
Do your swimsuits stretch much?
Our Sirens will have a small amount of give in them as you wear them. But all our swimwear pieces are lined with a very high-quality power mesh lining, so only a small amount of stretching may occur. We recommend you select a style & size that is comfortable for you now.
Try@Home FAQ
What if I decide I want to keep something that wasn't in my original Try@Home Order?
No Problem! Just complete your Returns Form and include the style you'd like in the Exchange section. Send all Swimsuits back to us, and we'll swap them for your chosen style.
What if I don't want to keep any of them?
Didn't find your perfect swimsuit? That's ok, you can either send them all back to us or exchange them for another 3 Swimsuits.
As we want you to be fully satisfied with your online purchase; we will happily exchange, refund or credit any swimsuit that you are not 100% happy with.
Your purchase is eligible for a Refund, Credit or Exchange provided:
- It is posted back to Sirens within 5 days of delivery.
- Is unwashed with all of the garment tags & hygiene stickers intact.
- Sale items are not eligible for a refund (see sale policy below)
To get your Free Returns Label
You will have received tracking emails from us during the delivery process. At the bottom of that page you will find the section to receive your free return label. Alternatively login in and find your unique tracking number and enter it here: https://app.starshipit.com/track.aspx?code\
If all items are returned without a purchase you will be required to pay a $10 restocking fee.
Note that returns from New Zealand and other overseas countries are at the customers expense. Please take to your local post and return to our warehouse.
SALE ITEMS Terms & Conditions
Try@Home deposits used to buy sale items are not eligible for a refund. Sale items bought outright with Try@Home are also not eligible for refunds however, we will issue a store credit (valid up to 12 months) that can be used on single item purchases.
Note that store credit cannot be used on Try@Home Orders.
All Try@Home packages must be posted back within 5 days of being received.
Sale items are only available to Australian and New Zealand customers.
If you've received a voucher as a gift it can be redeemed at any time. Gift Vouchers are not redeemable for cash and cannot be refunded via alternative payment methods.
Once your package has been received, your refund or exchange will be processed within *7 business days. To avoid any further delays please ensure your Return Form is completed correctly.
*Delays may occur during SALE or peak periods such as Christmas, New Year & Easter.
Your deposit is applied to any kept items (if you are using Try@Home). If there is a cash refund, it is refunded to your credit card upon return being processed by our warehouse.
Our Sirens Swimsuit Promise
We love and believe in our swimsuits and we want you to as well.
To show you the love, we're proud to offer you a 6-month product guarantee against all manufacturing faults and defects with normal use and correct swimsuit care. This means we will happily repair (or replace if necessary) any item that proves to be faulty or defective within this time frame!
However, we are unable to extend this promise to swimsuits that have been misused or cared for incorrectly.
To ensure your swimsuit lives a long and happy life, you'll need to care for your swimsuit according to its care pages & the instructions below
How to love your Sirens Swimsuits:
Hand wash your swimsuit thoroughly after each wear. Use cool water and mild soap or chlorine neutralising swimsuit wash.
- Do not wring, roll or store wet.
- Dry it in the shade. Never tumble dry or hang in direct sunlight.
- Do not allow sunscreen to come into contact with the straps or binding.
- Avoid rough or abrasive surfaces. These will cause the fabric to pill or snag.
STILL HAVE QUESTIONS?You can email firstname.lastname@example.com
Our Sirens Swimsuit Promise
We love and believe in our swimsuits and we want you to as well.
To show you the love, we're proud to offer you a 6-month product guarantee against all manufacturing faults and defects with normal use and correct swimsuit care. This means we will happily repair (or replace if necessary) any item that proves to be faulty or defective within this time frame!


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Cheese and mushroom casserole with chicken
This casserole is simple and delicious, and makes a perfect snack during a picnic (bento anyone?)—you should use it as a basic idea for a casserole, since the pasta sauce is the stable and the most important piece of the meal. It's also a very tweak-able recipe! :-)
Creamy scrambled eggs
I love the creamy texture of scrambled eggs, but I strongly dislike the idea of eating eggs raw, so I hacked a scrambled eggs recipe to be both completely set, creamy, and pack a crunchy punch as an extra. It's so delicious with fresh bread or toast, and the consistency makes it spreadable like a cottage cheese! I come back to this recipe quite often, because it's simple, fast and feels luxurious ;-)
Sweet potato quiche
Quiche is that one recipe that I love coming back to — it's easy to make, looks delicious and tastes heavenly :-) Plus, it allows me to come up with new fillings, which makes it very versatile!
Sweet potatoes are very smooth and creamy when boiled, then baked, and shallots add a crunchy texture. The combination of them is very mild and aromatic, so make sure you spice it up to your liking!
German Zimtsterne (cinnamon almond cookies)
I always wanted to make these, and finally got the guts — oh boy, I wasn't really prepared for the amount of nitty-gritty with making these. I started with original German recipe that called for 500g of almonds, but that ended up being 2 full baking sheets and lots of sneaky snacking between steps ;-) But the result is good, and these cookies are worth the effort.
Simple chicken teriyaki bento
This recipe is very easy and fast to cook, because it requires ready-made teriyaki sauce. Still, it's a really tasty alternative to sausages and pork, and chicken made in teriyaki style is juicy, tender and full of aroma! It also takes just few minutes, because the meat is first fried quickly, and then glazed with sauce. Fast food like a boss ;-)

subset: commoncrawl


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Few Republicans turn out for Obama's second inauguration
Republicans weren't easy to find but they were among the estimated 800,000 people to turn out for President Barack Obama's second inauguration Monday.
"Ultimately, this is about the country, our nation," said Delia Jennings, a West Virginia native who voted for Mitt Romney. "I can't change it, so I might as well be a part of it. You look at all the people stretching back all the way to the Washington Monument, I mean how can you not want to be a part of this?"
Jennings attended the ceremony with a friend, Victor Parker, who is a Democrat. Normally, the two chat about the government, among other things, but not at the inauguration.
"We're not talking politics today," Parker said.
Plenty of GOP members skipped the inauguration by getting out of Washington, D.C., altogether.
Charlie Spies, a prominent Republican lawyer based in the District, organized a trip to Las Vegas for nearly 100 Republicans.
The group is relaxing at the Wynn resort and going steakhouse-hopping.
"The trip is going great," Spies said in an email Sunday night.
Back at the inauguration, a few rows from the presidential lectern, Arlington resident Louise Buchanan laughed at the question of whether she voted for Romney.
"I salute the Republicans who are here," Buchanan said. "That's how it should be."
But Buchanan, a strong Obama supporter and lifelong Democrat, knows what it's like to be at the inauguration she too wants to see elected.
In 1989, she sang in the Congressional chorus on stage at President George H.W. Bush's swearing-in.
"It was a thrill to be here at all," Buchanan said. "We really weren't that focused on who the incoming president was. I just have such strong respect for the office of the presidency."
Some Republicans at Obama's inauguration shared that same sense of respect.
Rep. Paul Ryan, R-Wis., who would have been vice president had Romney been elected, told reporters last week, "it's my obligation" to attend.
One of Obama's more high-profile political rivals, House Speaker John Boehner, planned to attend a tea at the White House and a post-inauguration Congresisonal lunch at the Capitol, according to spokesman Brendan Buck.
"You want to know what I'm doing inauguration day?" said a man who answered a phone in Boehner's office. "I'm working."


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Lethbridge Open Houses returning as the Alberta Real Estate Association lifts its ban on open houses. Lethbridge Open Houses many implementing new consumer safety policies, regulations, procedures to prepare for lifts ban on Lethbridge Open Houses. Selling or buying a home has gotten a lot more difficult but Lethbridge open houses are now open.
Lethbridge COVID-19 Face Coverings Bylaw Updates with a growing number of instances new open houses safety policies, there are various questions and concerns during the pandemic.Frequently Asked Questions
Make your Lethbridge Open houses are as accessible as possible, removing unnecessary furniture and retaining a small amount of personal items such as family photographs. The house should speak to potential buyers by presenting itself as a place others could easily call home.
livestreaming platforms such as Facebook live, Zoom, YouTube and Instagram live are available to view Open houses from the comfort of your own home.
Lethbridge Broker open houses are promoted exclusively to the brokerage community, very effective in educating agents on upcoming Lethbridge open houses.


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We're searching the KWIPPED supplier network for food depositor inventory.
KWIPPED is an equipment rental marketplace. We'll send your request to our supplier network and they will provide quotes.
KWIPPED suppliers are ready to provide custom quotes for your food depositor rental.
We just need a little more info...
Speak with a KWIPPED representative at (800) 273-8404 (9-5pm ET), email [email protected] or chat with us below:
KWIPPED offers unparalleled efficiency to source equipment rentals and leases from a global network of equipment suppliers.
Tell us where to send your quotes.
Eventually, we'll need a few more details to facilitate your rental/finance, but we know your time is valuable.
Do you want to submit the request now, or do you have some time to enter company details?
Upon letting us know the information below, you will have full access to KWIPPED's platform.


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Christian thriller novelist Mike Dellosso knows all about living through nightmares. Here's the gripping story behind his stories.
Mike Dellosso believes in monsters. He's seen them. They've shown up in the eyes of an alcoholic loved one, a daughter battling a rare blood disorder and his own bout with colon cancer. He's had to fight them for his life, and he hasn't always been sure who was winning. But now that he's faced his worst fears and lived to tell about it, he refuses to look away.
For the last three years, those monsters have been showing up in his supernatural thrillers as beasts that roam dark woods (Darlington Woods), towns with dangerous secrets (The Hunted) and otherworldy screams that warn of untimely deaths (Scream). His fourth novel, Darkness Follows, about a heartless killer and the secrets unearthed through an old Civil War journal, released in May.


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Pollen analysis - Pont-du-Navoy (F-39), lower river terrace of the Ain river
Natural site. Geological section in pleniglacial varves. 3 palynological samples.
EBSD mapping of shear zones strain gradient - deformation mechanisms
Objective : characterize the deformation mechanisms and the crystallographic fabrics within homogeneously deformed granitic samples.
EBSD mapping of brittle shear zones precursors
Objective : microtextural characterization within a shear zone brittle precursor
EBSD mapping of shear zones strain gradient - seismic anisotropy
Objective : calculate the crystallographic preferred orientation (CPO) of each mineral phase in order to rebuild the elastic properties of the polyminéralic aggregates.
Microprobe analysis on a brittle shear zone precursor
Objective : compare the chemical compositions of minerals within the brittle precursor and in the host rock.
Quantitative X-ray mapping of brittle shear zones precursors
Objective : characterize the chemical zonings in plagioclase and garnet related to fluid-rock interactions within a shear zone precursor
OMIV - Time series of landslide surface displacements measured by GPS/GNSS
GPS/GNSS observations acquired on OMIV landslides at 24h/30s session/frequency in Rinex standardized file format. Time series of sensor positions and baselines (E, N, Up components).
OMIV - Time serie of seismic waveforms recorded on landslides and catalogue of endogenous seismic events
Seismic waveforms continuous records acquired on OMIV landslides at 250 Hz in miniseed standardized file format. Instrumental catalogue of endogenous seismic events.
OMIV - Time series of meteorological parameters on landslides
Meteorological observations acquired on OMIV landslides using a standardized protocol (6min, 15 min, 30 min, 60 min) according to the measured parameters and transfered in a Ascii standardized file format with the file database management system BD-OH OMIV. Estimation of potential evapotranspiration ...
Mass screening for Alveolar echinococcosis in south Gansu, China
Data related to human population screening and dog surveys carried out in 1996-97, 2005-6 and in 2014/15 in South Gansu, China
DCF77 time of flight measurement
DCF77 is a Very Low Frequency (VLF) emitter located in Mainflingen, Germany, about 400 km away from Besançon, France. The objective of the collected data is to measure the time of flight of the 77.5 kHz signal with respect to GPS 1 PPS used as reference, and assess the dependence with ionospheric ...
Structural dataset in the Montagne Noire migmatitic dome (French Massif Central)
The structural dataset gives the orientation of structural elements (bedding, cleavage, foliation, lineation, etc.). The data are georeferenced in the World Geodetic System 1984.
Wetland functionning and functional traits of Nuphar lutea
Eleven populations of Nuphar lutea were sampled in eleven wetlands differing in physicochemical characteristics and spread along three rivers (the Ain, the Doubs and the Loue Rivers). Fifteen traits, grouped into four sets (growth, reproduction, storage and defence), were measured during the growing ...
Pollen analysis - Flims (Suisse, Grizon), Sturzstrom
Section in the holocene caving of Flims. 6 sterile palynological samples.
Cholera database of Democratic Republic of Congo
This database contains information on the weekly monitoring of cholera in the health zones of the DRC since 2000.
DNA Library - CNR Echinococcoses
DNA Library is a database associated to a web interface, created to improve the storage and the life of a biological sample collection, closely related to the research activities of the National Reference Center for Echinococcosis (CNR Echinococcoses, University Hospital of Besançon, France). A ...
EWET : EmsB Website for Echinococcus Typing
The EWET database (EWET-DB) contains genotyping data on the parasite Echinococcus multilocularis, obtained after the analysis of the microsatellite EmsB. A set of information is also avaible on the sampled parasite isolates (host, geographical position, sampling conditions, laboratory of reference, ...
Pollen analysis - Saint-Girod (F-73), Argiles d'Albens (Les Epinettes ?)
Natural site: section. 6 palynological samples. Top of the section in the "argiles d'Albens" (Albens clays)
Jurassic Karst
Hydrogeochemical monitoring of karst springs in the Jura mountains
Pollen analysis - Saint-Pierre-de-Curtille (F-73), Lac du Bourget, deep drilling (LDB 01-I)
Natural site: drilling in the deep levels of the lake (Edytem laboratory). 2 palynological samples.
Pollen analysis - Labergement-Sainte-Marie (F-25), Between the two lakes. M. Magny/V. Bichet Transects
Natural site. Peat bog between Saint-Point lake and Remoray lake. 10 palynological samples + 15 radiocarbon datings.
Pollen analysis - Saint-Pierre (F-39), Sur les Grays
Natural site, wet meadow. 13 palynological samples. Lacustrine chalk-peat limit. Approximate geographical coordinates.
Pollen analysis - Onoz (F-39), Lac d'Onoz
Natural site: wetland border on the lake. 81 palynological samples by Hervé Richard and 81 samples by Jacques-Louis de Beaulieu (Marseille). No radiocarbon dating. Never published. Other analysis by Carole Bégeot (2008).
Pollen analysis - Ney (F-39), Varves de Ney, Les Prés des Rousserots
Natural site: geological section border on the Ain river. 2 palynological samples.
Pollen analysis - Villers-le-lac (F-25), Le pont (forage hydrogéo)
Natural site: lacustrine drilling. 2 palynological samples.


text: If you only have PDFLib Lite installed, I would not recommend bothering with this library, as you can really only output text and import an image, and that's about it. Forget about adding complexities such as color, blocks and other elements. Switch to an open source library such as FreePDF (http://www.fpdf.org).


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HR 384 - Oversight of the Troubled Asset Relief Program - National Key Vote
- Referred to Committee
- Jan. 22, 2009
- House Bill Passed
- Jan. 21, 2009
- Introduced
- Jan. 9, 2009
Related Issues
Stage Details
See How Your Politicians Voted
Title: Oversight of the Troubled Asset Relief Program
Vote Smart's Synopsis:
Vote to pass a bill that creates accountability measures for the Troubled Assets Relief Program (TARP) funding.
- Increases reporting requirements and procedures for companies receiving TARP assistance in order to better determine how the funding was used (Secs. 101 & 103).
- Restricts the structure and compensation of executive boards of companies using TARP assistance (Sec. 102).
- Makes TARP funding available to small community financial institutions designated as S-corporations and C-corporations (Sec. 105).
- Establishes the Office of Minority and Women Inclusion to ensure women and minorities are included in TARP companies (Sec. 107).
- Provides at least $100 billion for foreclosure mitigation, unless the Secretary of the Treasure deems the need to be lower, in which case the amount needs to be at least $40 billion and reasons for a lower estimate must be reported to Congress (Sec. 201).
- Recommends up to a 9 month moratorium on foreclosure proceedings and uses the HOPE for Homeowners Program to mitigate foreclosures on affordable housing (Secs. 207 & 208).
- Extends and restructures bridge loan for automotive companies (Sec. 302).
- Authorizes the Secretary of the Treasury to establish or support the availability of consumer loans, municipal securities, commercial real estate loans, small business loans, commercial loans, and automobile fleet purchase loans (Secs. 401 - 406).
- Establishes low interest loans as a "home buyer stimulus program" through the HOPE for Homeowners Program (Sec. 601).
- Increases the amount of money insured by the Federal Deposit Insurance Corporation (FDIC) from 100,000 to 250,000 (Sec. 701).
Title: Oversight of the Troubled Asset Relief Program


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FACULTY HONOR: Bassler elected associate member of European Molecular Biology Organization
June 18, 2013 4:30 p.m.
Bonnie Bassler, Princeton University's Squibb Professor in Molecular Biology and a Howard Hughes Medical Institute Investigator, was among 52 life-sciences researchers elected to the European Molecular Biology Organization (EMBO). Bassler and nine other researchers from outside Europe were named associate members of EMBO, which works to encourage promising researchers and foster a supportive, cooperative research environment in Europe. EMBO members provide suggestions and feedback on the organization's activities and help mentor young scientists.


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With almost 30 years of experience in the roofing and gutter industry, O'Boyle's are experts.
Contact us for a FREE assessment and quote or call 03 9718 2033 today!
Whether a recent hail storm punctured your roof or you just noticed a leak, the professionals at O'Boyles Roof Plumbing can help. O'Boyles has been in business for almost 30 years, helping businesses and homes with any and all roof repairs in Kew East.
Here at O'Boyles, we specialise in metal roofing materials. All materials we use are Australian Bluescope Steel, Colourbond or Zincalume. Because we provide high-quality craftsmanship with these proven materials, we give our customers the most reliable repairs in the industry.
You probably know that extensive roof damage can require restoration or roof replacement. But what about the small issues?
We can eliminate most everyday roofing problems through straightforward roof repairs. The damage our repair team handles includes:
If you notice any of these problems, do not wait to call O'Boyles.
Letting the issue sit and develop may cause structural issues, extensive corrosion or other serious roof damage. Once the problem becomes advanced, it may require more extensive, and more expensive work such as roof replacement. Let us handle the issue now, quickly and efficiently.
We've provided homeowners, business owners and industrial site managers dependable roof repairs and roof replacement in Melbourne for over 30 years. Our staff comply with all relevant licensing and certification requirements, including complete plumbing licence. Whether your roof has a small leak or missing tiles, trust our technicians.
Our head office is located in Diamond Creek, but we also have team members stationed throughout Melbourne for your convenience.
With almost 30 years of experience in the roofing and gutter industry, O'Boyle's are experts.
Contact us for a FREE assessment and quote or call 03 9718 2033 today!


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New Single By Tinariwen Premieres At Guardian UK
The new album Tassili by the storied Malian band TINARIWEN will be in stores August 30th via Anti- Records. The very first single from the album, a seductive and haunting African blues called "Tenere Taqqim Tossam" features TV on the Radio's Kyp Malone and Tunde Adebimpe, and is now premiering exclusively at the Guardian UK website.
On their latest Tassili, the acclaimed band has returned to the very essence of their sound. The album was recorded in the Algerian desert with only acoustic guitars and unamplified percussion along with contributions by Tunde Adebimpe and Kyp Malone of the group TV On The Radio. Later on guitarist Nels Cline (Wilco) and the horns of the Dirty Dozen Brass Band joined in to create an album that reaches deep into the essence of Tinariwen's sound while simultaneously opening itself to the surrounding world.
"Incorporating Algerian rai, Egyptian pop and Led Zeppelin, their last album, 2009's Imidiwan, won an Uncut award for being "the most inspiring and richly rewarding album of the last 12 months". - Guardian UK
To listen to the brand new song "Tenere Taqqim Tossam" at the Guardian UK site, just go here
TINARIWEN will be embarking on a long awaited North American tour beginning today, July 7th in Chicago.
TINARIWEN Tour Dates:
Jul 07 Lincoln Hall Chicago, IL
Jul 08 Cedar Cultural Center Minneapolis, MN
Jul 09 Winnipeg Folk Festival Winnipeg, MB
Jul 10 Winnipeg Folk Festival Winnipeg, MB
Jul 12 Belly Up Solana Beach, CA
Jul 13 Troubadour Los Angeles, CA
Jul 14 Bimbos 365 Club San Francisco, CA
Jul 16 Neumo's Seattle, WA
Jul 17 Vancouver Folk Fest Vancouver, 02
Jul 20 HighLine Ballroom New York, NY
Jul 21 Grassroots Festival New York, NY


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Our Mission
Our Programs
Our Results
Photo Gallery
How to Help
About us
Contact Us
Co-Founder's Message
As a small organization, we are challenged with ensuring that each donation makes a substantial impact on the lives of the children we help. Our responsibility is to ensure that your donations have a maximum impact and that we partner to identify areas where we can truly make a difference.
We continue to be inspired by individuals who selflessly dedicate their lives to the care of infants and children in countries that struggle with tremendous
poverty. Our programs and partnerships continue to grow to most effectively meet our mission. 100% of your donations go to help these children.
In January 2011 the CRF team made a visit to several of the organizations we support in the Philippines. Poverty continues to be appalling with slums sometimes hidden behind rows of Hollywood type facades built to resemble colorful stucco homes. Behind the fake walls are homes fashioned out of boxes, corrogated aluminium sheeting, discarded plywood, and bedsheets hung as makeshift doors and windows. Their shanties perched atop rivers of open sewage are the only home many of them know. The stories of the abandoned babies found crying on smoldering garbage, children trafficked, beaten and raped or sold by their parents as prostitutes continue. Our mission continues to support the real heroes who spend their lives defending those who have no voice. We are proof that through your help, a small organization can make a big difference.
Although we support a broad range of charitable efforts, our focus is on girls who have faced the most difficult of circumstances – girls who are victims of abuse, neglect, abandonment or trafficking – to provide them with exceptional opportunities for a bright future.
Our approach is twofold, first to ensure that these girls are cared for during their formative years through our Care packages and, secondly, to provide these girls, often dealing with issues of abandonment and neglect, the opportunity to be trained in professional careers. Empowering them and giving them the best chance for a better life.
We are privileged to partner with those who do so much for others.
For those of you who support us, on behalf of the children, Thank you! For those who want to make a difference, we welcome your assistance.
Please consider us in your next United Way giving campaign. You can also make donations directly on this website or through Universal Giving atwww.Universalgiving.org where you will find donations starting from only $10 for a bag of rice. We invite you to join us, one small step at a time…
CRF hosts Xmas event at local hospital
Dec 12, 2013, 12:22 PM
by Tia Razon
CRF hosted a Christmas celebration for children with different illnesses at the Quirino Medical Center hospital. Our partners, UHappy Events, had their volunteers engage the children and their families to a day of interactive storytelling and Christmas card making.
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Our Take: Current market conditions for selling your home by Krueger Realty
"We constantly monitor the specific market climates in order to help you make the best possible decision. Currently there are balancing factors. Although we believe there is a basic pent-up demand for homes, this is offset by some level of economic uncertainty. With local inventory remaining low and the percentage of distressed sales decreasing, prices should nudge upward."
The best time to sell is when you are ready, or when you must. That is, when you have outgrown the space in your current home, or you prefer to trade down to something smaller. Perhaps your marital status has changed, which necessitates a move, or you need to relocate for a job.
Market conditions also play a role, as do seasonal conditions. For example, your chances of getting top dollar for your home are more likely in a seller's market, when demand outweighs supply, than in a buyer's market.
Local and national economic factors also may dictate when to sell. If a major employer in your area is laying off workers, it may not be a good time to put your home up for sale.
People will be cautious about buying when the future appears unpredictable. Most agents agree the best time to sell is in the spring. This is when the largest number of potential buyers hit the market. Your home is likely to sell faster and at a higher price, although sales begin to pick up as early as February and start to slack off in July, the slowest month for real estate transactions.
We can help! Check "What is my property worth?" here.
Determine what is the best course of action in your particular case. Contact us, and we look forward to working for you.


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One time purchases and subscriptions are both one-click downloads.
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© 2018 Mark Anderson
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Maiden's of Telford is part of the Kinaxia Logistics group of companies.
Kinaxia Logistics is a leading Logistics Group within the UK haulage and warehouse sector. Businesses in the Group work together with the core purpose of enhancing customer service and increasing efficiency. Learn more here.
Modern Slavery and Human Trafficking Statement
Privacy Policy
© A.J Maiden and Son Limited. All rights reserved


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After the 2000 election, I read 3 books, published in 1971/1972, dealing with the inquiry into the Electoral College that grew out of the very close 1960 election. The great constitutional law scholar Alexander Bickel wrote one of the books, "Déjà vu: Reform and Continuity; The Electoral College, the Convention, and the Party System." Here's the PDF of the article I wrote about it.
Posner doesn't mention one benefit to the Electoral College that was considered very important back in the 1960s: the perpetuation of the 2-party system. From my article:
Although the electoral college does not eliminate third parties, it suppresses them. Only the geographically concentrated third party can gain electoral votes. If third parties have a role to play, one should argue that it is the third party that transcends state borders that is more likely to infuse the political debate with worthy new ideas; better a Henry Wallace than a George Wallace. Despite the disturbing ability of a third party candidate like George Wallace to make headway in the electoral college system by appealing to regional prejudice, that candidate did not succeed. The threat he posed within the electoral college system was overshadowed, at least for some observers, by the potential under the direct vote system for multiple candidates to jockey for position in a runoff or to seek to provoke a runoff and then bargain with candidates who might need to make deals or concessions to win in the runoff. Withdrawing the need to win a plurality in a state to acquire votes would energize third parties who tapped national popular issues. More candidates would enter the field, creating a greater likelihood of a runoff election and lowering the percentage necessary to qualify for the runoff. What if moderate candidates cancelled each other out, resulting in a runoff between two ideologues or extremists? What would stop major party candidates who failed to win their party's nomination from routinely joining the race? Instead of opening the democratic process to greater participation, one might end up with a small crowd of "demagogues, quick-cure medicine men, and ... fascists of left and right" who would collude among themselves.The perceived need to preserve the 2-party system led the ABA to identify 6 other reasons why it would continue without the Electoral College:
... (1) the tendency of an existing form to persist; (2) the use of plurality votes to determine the winners of single member districts; (3) public consensus; (4) a supposed American cultural homogeneity; (5) political maturity; and (6) the natural tendency toward dualism.Yeah, I know: what?! But that's what they said. You may wonder what's so damned good about the 2-party system. But a key point was: You can't amend the Constitution without the votes of the present members of Congress and the state legislatures, and these folks are all there as a consequence of the 2-party system. The argument they made out loud was that the 2-party system produced stability and moderation.


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Daniela Mehech 18:29, 13 August 2010 (EDT)
Daniela Mehech 21:17, 12 August 2010 (EDT)
Tuesday (8/10):
Transformed all goal parts.
Paused assembly on 4 remaining parts
Wednesday (8/11):
Picked 2 colonies for all goal parts
did not pick colonies for Igem 107 in CA
Thursday (8/12):
Mini-prepped all goal parts except 4
Picked colonies for B3 in plates A and B, and C3 and D3 in plate A
To Do Friday:
Mini-prep those 4 parts
RE map everything
Daniela Mehech 17:34, 9 August 2010 (EDT)
Friday (8/6):
Threw out 107, 109, 115, 116 because we plated them on the wrong antibiotics. Need to redo their assembly mini-prep, RE, sequence 130, 135
Add terminator to 112, 113
Saturday (8/7):
pick colonies for 107, 109, 112, 113, 115, 116, 126
Sunday (8/8):
mini-prep 107, 109, 112, 113, 115, 116, 126
Monday (8/9):
Assemble 128, 129
Transfer 107 to CA (in order to assemble 127)
Plate 140 on arabinose
Transform 112, 113, 115, 116 into lefties (we were using righty methylated minipreps when trying to add the terminator)
Daniela Mehech 19:25, 5 August 2010 (EDT)
To Do Thursday:
Analyze sequencing data
Mini-prep, RE, sequence 124, 125, 134, E/B transfer
Transform and plate 125, 126
Assemble and transform GFP with ig114
Redo 107, 109, 112, 113, 115, 116, 130, 135
Daniela Mehech 19:33, 4 August 2010 (EDT)
Mini-prep and Map Catch ups
send in sequencing 133, 136, 138
pick colonies 124, 134, E/B transfer for ig114
Manual Assembly 125, 126
Add terminator 107, 109, 115, 116
regular assembly 130, 135, 112, 113
Daniela Mehech 17:15, 3 August 2010 (EDT)
August 2nd:
Most of Stage 4 parts come out with good sequencing.
ig114 has two bp mutations that got passed on to just about every single part. The mutations shouldn't affect the function but we're going to test that to make sure
b1006! is missing from various parts because of iGEM 66 failure. We're going to add those to the ends of the parts
A couple of parts did not work and we can't figure out why. We'll remake their parent parts and try again
igem111 that was in Laeretes was wrong. We put in a different copy from Gandalf in Laeretes that sequenced correctly. We're re doing those parts
igem 92 was wrong. We're redoing it and those parts
Transformed the parts we need to remake
August 3: Picked Colonies for parts that transformed
Three parts didn't grow so we made them by hand. Not sure if the bands in the gel came out the right size
We have 11 sequence confirmed goal parts!
Daniela Mehech 19:53, 30 July 2010 (EDT)
July 28:
Mini-prepped and did RE map
RE map came out bad for almost all of Stage 4 parts. For some reason a lot of parts were not being added.
Sent in Stage 2 and Stage 3 parts that had small parent parts (because RE mapping isn't sensitive enough to show us if small parts were assembled correctly)
Picked more colonies from Stage 4
Re-did stage 4 and included some Stage 4 Catchups
July 29:
Mini-prepped Stage 4 Round 1 re-picks
Picked colonies for Stage 4 Round 2
Analyzed sequencing results - apparently iGEM 66 never took on it's righty parent part and everything that came from igem 66 is missing the terminator
July 30:
Mini-prepped Stage 4 round 2
Did RE map of Stage 4 round 1 re-picks and Stage 4 round 2
RE map is still questionable so sent in all of Stage 4 that we had for sequencing
Daniela Mehech 17:55, 27 July 2010 (EDT)
July 26: Did stage 4 assembly and Stage 3 Catchups
July 27: Most things grew except for igem119 and igem104. igem113 sequenced wrong so needs to be redone
Picked colonies for things that grew, but did not start catchups just yet
To Do
Mini-prep everything
Do Stage 3 cathcups (106 and 104) and Stage 4 catch ups that we can do without 106 and 104 (about 6 out of 10)
sequence some goal parts
Do last of stage 4 catchups
Daniela Mehech 14:46, 23 July 2010 (EDT)
July 22: Mini-prepped re-do's of stage 1 and what we had for stage 2. RE map came out good (see Christoph's notebook). We made a plate of all good Stage 1 and Stage 2 parts.
Picked colonies for sbb18, igem64 and igem65. These still need to be added to our final plate
Today: Mini-prep and RE map sbb18, igem64 and igem65, and added them to final plate. See RE map below
Did Stage 3 and stage 2 assembly catchups
Daniela Mehech 19:12, 21 July 2010 (EDT)
Most of Stage 2 parts grew although very sparsely. Picked colonies for them, will mini-prep tomorrow morning. We will re-do the 4 missing parts when we get RE map and co-transformation plate results, that way we only have one catch-up group
Picked colonies for stage 1 re-transformations. They all grew except for iGEM65 because we used DNA from the wrong well. Transformed iGEM65 again using DNA from Rosencratz
iGEM64 did not grow with stage 2. Doing assembly by hand (separate digests, gel purified, zymo column). We're re-transforming sbb18 in AK from Mad Hatter to try to get a better mini-prep. If manual assembly still doesn't work we'll have to try again with new sbb18 mini-preps
To Do
Mini-prep stage 2 and stage 1 parts. RE map.
Make new plates for final stage 1 and 2 parts.
Do Stage 2 catch-ups
Pick colonies for 65,64 and sbb18
Daniela Mehech 14:48, 20 July 2010 (EDT)
Christoph picked colonies, mini-prepped, ran RE on Stage 1 parts and did the catchups. Check his page for gel pictures. We have all the stage 1 parts except for one where we used the wrong parent part twice. We did Stage 2 on yesterday. Today none of the rigthies grew and 5 of the 6 Lefties grew.
possible sources of error:
-put antibiotics in wrong location on robot
-Ophelia layout is wrong
-DNA was too dilute
-Cells were too dilute
to do:
-check Ophelia's layout
-col PCR on things that did grow
-Re do stage 2 with higher DNA and Cell concentration
Colony PCR of what did grow from Stage 2 Round 1:
Daniela Mehech 23:27, 12 July 2010 (EDT)
July 10: Broke my back; will return to lab later this week. Christoph doing project solo for now
July 9: Transformed cells and plated them
Daniela Mehech 16:46, 8 July 2010 (EDT)
July 7: Picked 4 colonies for each part. Re-made 7 parts that did not grow well/at all
Sent 7 with 10 to sequencing
66% of parts had all 4 colonies co-transformed. We've gone back to troubleshooting
7 with 10 sequenced perfectly, but we've left them in the tubes instead of moving them to the plate
Doing a RE digest to see if parts are methylated correctly (these are all lefties):
Another RE digest:
Triple Digest
We began assembly again, this time with more dilute DNA to see if this helps reduce co-transformation. We did digestion and left the ligation over night
Daniela Mehech 21:29, 6 July 2010 (EDT)
RE mapping of 3 parts that we repicked (6 with 24 in AK, KA and 7 with 24). They all came out faint, but most look good)
Did stage 1 assembly without a problem.
Picked Colonies for 7 with 10. Colony PCR results shown below; they all look good
To Do
Check colony PCR result for 7 with 10, mini-prep pick colonies, colony PCR, streak and inoculate for stage 1 assembly
Daniela Mehech 16:42, 5 July 2010 (EDT)
Re-digested parts that showed up faint or not digested:
Everything came out good the second time with the exception of 7 with 10. We noticed that we've been using a mini-prep of 7 with 10 that had a bad sequencing result. We did a lefty transformation of the 7 with 10 that mini-prepped correctly
We chose to repick colonies from 4 parts that did not show up correctly on the RE map at all.
To Do
Pick colonies from 7 with 10 Mini-prep new colonies
Daniela Mehech 15:32, 2 July 2010 (EDT)
Only one colony from the manual assembly (out of 32) was not co-transformed (the same one that had the correct band in the colony PCR gel)
RE map of Methylated Parts for Stage 0:
To Do
Decide from RE map gel which parts need to be re-digested, re-picked or re-transformed
Daniela Mehech 17:27, 1 July 2010 (EDT)
Colony PCR of igem10_064 when Christoph and I made it by hand. Only one lane has a band at 1200. We mini-prepped all of them using the 8-strip method.
We picked colonies for all of our new methylated strains. Except for B4, nothing grew on that so we transformed
To Do
Mini-prep methylated parts RE map and analyze gel. Make plate with only correct parts that have been confirmed by RE mapping Pick colonies for B4, mini-prep at end of the day Check for co-transformation
Daniela Mehech 17:48, 30 June 2010 (EDT)
We used the wrong plate layout when we were remaking our parts - none of them work and we had to redo them.
Our co-transformation experiment didn't work (next to nothing grew), so we're changing it and doing it again.
We decided to start over from our unmethylated plate - we transformed every part into Righty/Lefty strains
To Do
pick colonies from re-made parts. Mini-prep at end of the day
pick colonies from co-transformation experiment and streak for triple resistance. Mini-prep at end of the day
Mini-prep the one part that was correct (6 with 23) and RE map it
Daniela Mehech 17:42, 29 June 2010 (EDT)
All 16 colonies of all 3 parts that we picked were co-transformed. No matter how many times we do it, either manually or with the robot we keep getting a high rate of co-transformation. We're going to test transforming with different concentrations of DNA, different amounts of cells, and a higher concentration of enzyme.
We transformed more parts (those that we are still missing and that did not have enough volume in plate B). They should be done by Wednesday, then we can do robot assembly on Thursday. By Thursday then maybe our experiment will we show us what concentration of DNA/cell/enzymes work best to minimize co-transformations
To Do
Check all of experimental parts (igem10_072) for co-transformation (pick colonies and streak)
Pick colonies, mini-prep, RE map and add newly made parts to dilution plate
Daniela Mehech 19:13, 28 June 2010 (EDT)
Began making dilution plate - transferred 20 ul of parts from either plate B or plate A (whichever had better RE map). Some parts though did not have enough volume in plate B and hadn't been RE mapped in plate A. We're just going to re-transform a big patch of parts so we know for sure that they're correct and that we have enough volume.
Our sequences for vectors pmLL8 and pmLL9 were switched, which explains why we thought we had so many parts wrong. Almost all of the parts in plate B are correct but we can't use half of them because we don't have enough volume. We're not using parts from plate A that were not RE mapped either. We're re-transforming parts and re making them. Now that we've determined that for the most part we were using the correct parts we need to keep looking for an explanation of why our stage 1 parts did not come out right.
Tim picked colonies for us from the parts that re-transformed on Friday. We mini-prepped them and RE mapped. Now that we corrected our sequences all the mini-preps came out correct and we added the newly-made parts to our dilution plate.
We picked colonies from the composite parts we're making by hand. At the end of the day we could tell everything was co-transformed except for igem10_076. It only had 5 colonies grow. We mini-prepped all 5 colonies and picked 16 more colonies for the three other parts.
To Do
re-make parts that we did not have enough volume of in plate A and that we just don't have at all (7 with 10)
Check for co-transformation in manual parts. RE map igem10_076 to determine if it is correct
Daniela Mehech 17:54, 25 June 2010 (EDT)
Transformed 7 unmethylated parts into Righty/Lefty strain. Left them in incubator for someone to remove them tomorrow
Did manual assembly of 4 parts (the 4 goal parts in stage 1, but we transformed them into Righty)
Made plate layouts and wrote CSV files in preparation for robotic assembly. We are not going to having any mislabeled wells this time
To Do
Pick colonies and mini-prep (Monday)
Transfer DNA to new stock plate (Monday)
RE Map all of new plate and sequence some parts to ensure that everything is where it should be (Monday/Tuesday)
Repeat Stage 1 Robotic 2A assembly (Tuesday/Wednesday)
Daniela Mehech 18:42, 24 June 2010 (EDT)
Most of plate B mapped correctly. We mapped the parts from plate A that mapped incorrectly on plate B:
To Do
Re-make those parts that mapped incorrectly in both plate A and B (just transform unmethylated parts into Righty or Lefty strain) Do manual 2AB assembly of four parts to determine if the procedure works, at least by hand Make new methylated stock plate (take best parts from plate A, B and the new ones that we make)
Daniela Mehech 16:23, 23 June 2010 (EDT)
We are going to start over with our mehtylated parts so we ran a RE-mapping of our original methylated parts plate to determine if that plate is correct.
Restriction map of methylated parts Plate B:
Part 1:
Part 2:
All the colonies I repicked were co-transformed, so I didn't run the ColPCR gel
RE mapping of random sampling of methylated and unmethylated stocks. Most are right, but a few came out wrong
Daniela Mehech 20:34, 22 June 2010 (EDT)
Mini-prepped selected parts from groups 3 and 4. Some parts were co-transformed/colony PCR showed no good clones. I repicked more colonies for those parts. Did a restri
ction map of the mini-prepped parts. Everything came out with 3 bands. Maybe the digestion did not go to completion.
Because we are getting such a high failure rate Christoph and I started looking over our entire procedure to see if something wrong happened at the beginning. A RE-mapping of our methylated parts plate did not perfectly match our plate layout = somewhere something went wrong and we need to start over
The top gel is RE-mapping of randomly selected methylated parts. The bottom gel is RE-mapping of mini-prepped composite parts
To Do
Look at Restriction Mapping results - decide what to re-transform and what to send for sequences and what to deem finished Look over repicked results (run colony PCR and check co-transformation plate) - decide what to mini-prep and what to retransform RE-map unmethylated parts to see if at least that is correct
Daniela Mehech 14:19, 21 June 2010 (EDT)
Colony PCR results from Group 2
lane 1,3,5,7 = 5 w/ 35 L
lane 2,4,6,8 = 5 w/ 38
lane 9,11,13,15 = 5 w/ 35 R
lane 10,12,14,16 = 6 w/33 L
lane 17,19,21,23 = 6 w/33 R
lane 18,20,22,24 = 9 w/ 8 L
lane 25, 27,29,31 = 7 w/40
lane 26,28,30,32 = 9 w/ 34 L
lane 33,34,35,36 = 9 w/ 8 R
lane 37,38,39,40 = 9 w/ 34 R
Except for first 8 lanes, everything looked good and we mini-prepped two of each. Mini-prepped lanes 1-8 too and will send them for sequencing
Colony PCR results for group 3:
Loaded as: 1,9,2,10,3,11,4,12,5,13,6,14,7,15,8,16,17,25,18,26,19,27,20,28,21,29,22,30,23,31,24,32
Colony PCR results for group 4:
Restriction Mapping of everything we've mini-prepped (groups 1 and 2)
Right Half
Left Half
To Do
Check for co-transformation for Groups 3 and 4
Mini-Prep good colonies of group 3 and 4
Send to sequencing some parts?
Begin Stage 2 (hopefully all our stage 1 parts are right)
Daniela Mehech 21:28, 18 June 2010 (EDT)
We labeled our transformation plate wrong so when we went to plate the cells many of them were plated on the wrong antibiotics. We still had colonies grow because they were co-transformed and had resistance to all antibiotics. This also explains why so many colonies grew when we tested for co-transformation. We have split our stage 1 parts into 4 groups:
1. Colonies grew on proper antibiotics and were not co-transformed. There are 12 of these. We mini-prepped these today and will do restriction mapping properly on Monday
2. Colonies that grew on wrong antibiotics need to be redone. There are 10 of these.We digested, ligated and transformed them today.
3. Parts that we thought we hadn't done (see yesterday's notes), but it turns out we did do them without realizing it since our plates were labeled wrong. Since we did them twice and they were all plated correctly the second time we will pick colonies, do Colony PCR, check for co-transformation and mini-prep these 9 on Monday.
4. Colonies that grew on the right antibiotics but were co-transformed. We will pick more colonies from the original plate to do ColPCR
Daniela Mehech 18:37, 17 June 2010 (EDT)
We got cell growth for every part we plated (although in some parts there were very few colonies, about 3 or 4).
We made a Colony PCR Mastermix and tried to distribute it to two PCR plates with the robot but the robot kept giving us an error message (It said it couldn't pipette 19uL if there was 17uL in the tube but there was 2 ml in the tube and we wanted it to pipette 17uL). We gave up and I pipetted all 136 wells by hand.
Christoph and I picked colonies, added them to the PCR plate, innoculated them in a 96-well plate and left them in the incubator. We ran a ColPCR3K program on the both PCR plates.
Christoph noticed that we had based all of our csv robot files from the wrong list. We made all of the parts we needed but some of the parts had to be inserted into multiple vector backbones. We began making the 6 forgotten parts by hand. 3 of these parts need to be transformed into both Righties and Lefties so we're making 9 parts by hand.
We dabbed broth from our 96-well inoculated plate on both AKC plates and LB-only plates as negative and positive controls respectively for whether we picked any co-transformed colonies.
We need to run a huge gel when the PCR finishes at 4:40pm. Then we need to analyze the results. Christoph already named all the new composite parts and calculated their sizes.
To Do
We will mini-prep our good colonies tomorrow. Hopefully we'll have at least two good copies for each part so that we can continue with Plates A and B for stage 2.
We can start planning for Stage 2 assembly when we have free time. If we do it beforehand hopefully we'll catch any mistakes before running the robot
We should make a list of all the information Clothos gives us after it solves for the assembly tree. We've had to spent a good chunk of time writing excel formulas and making spreadsheets just so we know exactly how to assemble everything
Daniela Mehech 18:03, 16 June 2010 (EDT)
Decided to redo all of B along with A. We can do them together on the robot by doubling everything but changing the source plate
Made dilution plate A, and added 60uL of dilution to dilution plate B. 7 with 11 still looked different
Distributed digestion cocktail to both dilution plates
Note: Robot begins pipetting air if it uses the same tip for two long. We stopped the program halfway through to change its tip
Distributed Righty and Lefty parts to reaction plates. Made sure there were duplicate columns for parts being transformed twice
Checked that at this point all wells had the same volume
Put plates in thermocycler to incubate and heat kill
Distributed antibiotics to 10 plates. All of LB agar was contaminated so we will pour that during the rescue step
added ligation mixture to each well and let incubate at room temperature
Began thawing cells (1 tube of each strain)
Poured agar plate
Skipped centrifuge step
Added cells to plate in two different concentrations (30uL, 70 uL)
Daniela Mehech 12:56, 16 June 2010 (EDT)
Robot 2AB assembly Stage 1
Yesterday we did stage 1 of our 2AB assembly only on plate B. Today we will repeat the process on plate A without making all of the mistakes that we did yesterday.
June 15:
Autoclaved our strips for plating. Learned how to work the autoclave machine
Made a new plate layout for the reaction plate. Wells are organized by what strain the part needs to be transformed into to
MISTAKE: Did not listen to Tim and organized wells by column instead of rows. Rows are better because the plating strips have 2 rows and 12 columns
MISTAKE: Did not read procedure all the way through in detail and assumed that we could use a well for two transformations. Our dilution and reaction plate needs to have duplicates of the parts that go into both Righty and Lefty strains. We have to add the duplicates in either the dilution or reaction plate
Made the dilution plate B (30ul of DNA into each well + 30ul of NEB2 stock diluted by a factor of 5). Dilution plate had the same layout as methylated stock plate so we did it by hand. Next time, we should consider adding duplicates of the parts being transformed twice.
NOTE: 7 with 11 was frozen in our methylated stocks plate. This is the part that was mini-prepped separately the day before. All other wells weren't frozen.
Had the robot make reaction plate (distribute digestion mastermix to all wells, distribute all Lefty parts, mix, distribute all righty parts, mix)
While the reaction plate incubated in the black thermocycler (which also did heat kill of the digestion enzymes) we distributed the antibiotics to the 24-well strips. We made antibiotics (dilute stock by 10, add 1ul of dye). This part went fine except we should have labeled the plates before we put them on the robot. Added LB agar to the plates and let them dry next to the flame.
MISTAKE: forgot to make second plate for parts being transformed twice. Column 5 in reaction plate needed to go to two different plates. We made the extra plate by hand
Had robot add the ligation cocktail to the wells and let it incubate at room temperature. We began thawing our bacteria.
Did transformation of bacteria. We had enough volume to seed at least 60uL of bacteria per column.
MISTAKE: Did not realize that the protocol was for seeding 10uL of bacteria. We wanted to seed about 150uL. We should not have done the centrifugation and aspiration of the supernatant step.
MISTAKE: ejecting tips from multi-channel before releasing cells on plate. We lost 60uL of our cells from column 7.
MISTAKE: We split the volume in column 5 into column 9 (because column 5 had to be transformed into both righty and lefty strains). Thus we also did not have enough volume for those parts.
MISTAKE: not taking a short break after lunch to re-energize and prevent making silly mistakes
To Do Today
- Run plate A and B together
- autoclave more 24-well strips
- Make dilution plate A and add volume to dilution plate B
- arrange reaction plate by rows instead of columns (have each row be transformed by the same strain)
- make more colored antibiotics
- label 24-well strip plates before putting them on robot and don't forget to make two plates for the parts being transformed twice
- do not centrifuge and aspirate supernatant after the recovery step
Daniela Mehech 20:37, 14 June 2010 (EDT)
Tim and Shelly picked colonies and mini-prepped for us over the weekend (Thank you!). We mini-prepped one colony (7 with 11), because nothing grew from our initial transformation. We also did RE mapping of 4 mini-preps to confirm that their tubes were labeled correctly (which they were).
|Lane #
|Expected fragments
|4 with 10 #1
|4 with 10 #2
|4 with 18 #1
|4 with 18 #2
|4 with 9 #1
|4 with 9 #2
|9 with 3 #1
|9 with 3 #2
Christoph and I began planning our Stage 1 assembly by writing a CSV file. We worked on replicating the spreadsheet Jennifer showed us to keep a detailed track of all of our parts.
To Do
Write final CSV file (both for making plates and for the digestion/ligation reaction plate) Make plates with proper antibiotics for plating Run Stage 1 on robot, transform, and plate
Daniela Mehech 19:04, 11 June 2010 (EDT)
Colonies grew from pMLL5+Bca1152 so I ran a colony PCR and inoculated 4 cultures. From colony PCR (band at 250), decided to miniprep colonies C and D. Sent D to sequencing, added it to parts plate (source) and transformation plate (destination). Mini prep from C went to Working Box
Added grown up Lefty and Righty strains to 500ml each of LB. Tim made competent cell stock for us
Finished with Christoph our CVS file for robot to dilute our parts in a new plate. (Note: when we were taking off the lids of the source plate, a few drops sprayed out. We dried the surface with a kimwipe, hopefully nothing got contaminated). Actually did the transformation by hand, but next time we should use the robot. Plated everything. Someone will move the plates from the incubator to the fridge tomorrow
To Do
Check that colonies grew in all of our transformation Get going on stage 1 of assembly
Daniela Mehech 14:38, 10 June 2010 (EDT)
Sequences for all parts we made were good (pMLL4+Bjh1882, pMLL5+Bca1091, pMLL6+Bjh2245)
Our competent cell cultures did not grow up at all - inoculated 2 more cultures of each strain and they appear to be growing well
Christoph and I double checked to confirm we had all the parts we need in the right vector - we're missing pMLL5+B10sbb37/Bca1152)
We took the part from the 140L stock, did a E/B digest, ligated it with pMLL5 digest and transformed it into jtk049 strain
I updated the pictures of the assembly tree
Began planning to do transformations of all our parts into righty and lefty strains
To Do
Pick colonies from Bca1152 transformation for colony PCR. Plate colonies in both CK plate and Spec plate. Nothing should grow in Spec plate
Make competent Pir cells
Transfer parts from working box to PCR plate
Write file for robot to do our transformations (first make dilutions from PCR plate)
When oligos come in - Biobrick upstream region of ef1-alpha
Daniela Mehech 15:33, 9 June 2010 (EDT)
For first Colony PCR of 1882 and 1091: Bca1091 pick lanes 8 and 10 for miniprepping.(Block #1: Colonies from well 1D and 2A)
For second Colony PCR of 1882 and 1091: Bjh1882 pick lanes 1 and 4 for miniprepping. (Block #2: Colonies from well A and D)
Did all 4 mini-preps (stored in working box) and sent in pMLL4-Bjh1882 (D) and pMLL5-Bca1091 (2A) for sequencing
Expected Colony PCR bands:
- pMLL4+bjh1882 = 849bps
- pMLL6+bjh2245 = 265bps
- pMLL5+bca1091 = 228bps
Colony PCR of pMLL6+Bjh2245 should be at about 265bps. Looks good-mini-preped lanes 2 and 3 and sent both for sequencing
Started growing up Lefty and Righty strains. Picked two colonies of each lefty and righty and grew them up in 10ml LB.
Designed three potential oligos for biobricking part Bjh2341CA (igemTen011, igemTen012, and igemTen013)
Bjh2341CA is a potential choano promoter (its a DNA region about 1kb upstream of ef1-alpha, a highly expressed gene)
- Finish generating competent cells for "Lefty" and "Righty" strains.
- Analyze sequencing data for pMLL6+Bjh2245, pMLL4+Bjh1882 and pMLL5+Bca1091
Daniela Mehech 18:24, 8 June 2010 (EDT)
Still working with Christoph
Did EB digest of pMLL6 and did gel purification. Did not take picture of gel because it looked right (a band showed up at approximately 2500)
Ligated pMLL6 digestion with Bjh2245 digestion from yesterday and tranformed into bacteria (not methylated strain, Jtk049)
Colonies appeared from our three transformations (Bjh 1882 with pMLL4and 2 samples of Bca1091 with pMLL5)
We ran a colony PCR on the 3 transformations but the results came out ambiguous (see picture below), so we picked 4 more colonies for more colony PCR
Lanes 1-4 are Bjh1882 and lanes 5-12 are Bca1091
Picked more colonies and ran second gel: Better results this time
To Do
- Miniprep colonies that we pick for pMLL4+Bjh1882 and pMLL5+Bca1091. These are methylated correctly for the robot. Restriction map for confirmation
- Colony PCR pMLL6+Bjh2245 in Jtk049. These are not methylated correctly. Possibly mini-prep if there is enough time
Daniela Mehech 18:58, 7 June 2010 (EDT)
Did the following with Christoph:
Eco/Bam digest of parts: Bjh2245 (LifeACT), Bjh1882 (RFP), and BCA1091 (Ptet)
Gel purified Bjh1882 and Bjh2245
Small fragment zymo cleanup of BCA1091
Couldn't see fragments. Turns out Bjh2245 is 97bp so we should have done a small zymo clean up instead of running it on a gel
Re-cut Bjh2245 and Bjh1882
Did a small zymo clean up of Bjh2245
Did zymo gel purification of Bjh1882 (see image below)
Next we ligated the digested part with the desired vector
Bjh1882+pMLL4 (AC)
Bjh2245+pMLL6 (KA)
Bca1091+pMLL5 (CK)
We don't have pMLL6 in digested form. We'll digest and ligate it tomorrow
We did transformation with Bjh1882+pMLL4 and Bca1091+pMLL5. We transformed them with a Lefty strain of E.Coli (Pir strain, yellow tube) so that the DNA is already methylated
To Do
Digest pMLL6 with Eco/Bam, ligate it with Bjh2245, transform check transformation of Bjh1882 and Bca1091


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Known the world over for his powerful photography, Steve McCurry was a natural choice for Silversea. Through his lens we travel off the beaten path and unearth the beauty and authenticity of our planet. A partnership that is built on one unique vision, it can only be Silversea.
Discover Nothern Europe through Steve's unique imagery.


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Because the sacrifices don't stop at the front lines we are also extending our Military Rebate offer to household members of eligible U.S. military personnel, including Gold Star Families.
Our Military Rebate is available to:
- U.S. military personnel (active-duty and inactive reserve)
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- U.S. military veterans (within 2 years of discharge)
To take advantage of the rebate, you must:
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Please stop by Herb Chambers Toyota of Boston in Boston, MA for more information. Herb Chambers Toyota of Boston looks forward to seeing you.


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OUHSC is built upon a rich tradition of innovative curricula and cutting edge research. In addition, OUHSC represents a unique opportunity to attend school surrounded by your future health care colleagues represented in seven different colleges: Allied Health, Dentistry, Graduate, Medicine, Nursing, Pharmacy and Public Health.
Welcome to the University of Oklahoma Health Sciences Center (OUHSC) located in Oklahoma City, Oklahoma, approximately 25 miles north of Norman, Oklahoma, and within blocks of the State Capitol.
The University of Oklahoma Health Sciences Center
1100 N. Lindsay, Oklahoma City, OK 73104 - (405) 271-4000
TECHNOLOGY: Information Technology | Service Desk
HIPAA: HIPAA Website | Notice of Privacy Practices | HIPAA Privacy Complaint Report Form
NOTICE: Information about FICA Refunds for Medical Residents.
Copyright © 2019 The Board of Regents of the University of Oklahoma. All rights reserved.
Disclaimer | Copyright | The University of Oklahoma is an Equal Opportunity Employer | Mission Statement
Built and Managed by: The OUHSC Web Team


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Objects in the shade of tan
We have 15,475 objects that overlap with this color. and this is page 1 of 443
The closest color to #c1b59a in the CSS4 color palette is
tan which has the hex code of #d2b48c.
Perhaps you'd like to see the intersection between this color and Crayola® or CSS3 palettes?


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Parent Directory | Revision Log
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Fix bug #506132 - Most architectures only have a single installation CD. Also, LiveDVD is not created every 6 months. Thanks to Vladimir Romanov
Fix bug #498570 - Checksum file contains different algorithms and confuses sha512sum
Bug 489782 - Switch links to wiki documents
Bug #489782 - LibreOffice, not OpenOffice (anymore)
Fix bug #466692 - Use sr0 as default example for CD-ROM/DVD-ROM devices
Fix bug #480416 - Suggest different hash calculator software as krylack now includes adware. Thanks to Ben Kohler.
Fix bug #479094 - Checksums are SHA-2 (512) now, so use sha512sum command
Fix bug #442686 - Book still referred to md5sum but only SHA-2 and Whirlpool is used
Fix bug #398281 - Update docs to refer to release engineering site for gpg keys
Bug #402073 - Use a more vague expression when dealing with release-specific data, this has the potential to change every week...
Portage tree is signed with new key (cfr mail on gentoo-dev-announce)
Fix bug #386477 - Mention both releng signing keys
update MILO options, bug 352453
update MILO boot stuff for bug 352453
bring alpha handbooks up-to-date for the autobuilds. new releases, so major version bump to handbook files. bug 292726. also add new file verification instructions for downloaded isos, bug 283402.
changed Alpha SRM boot flag, bug 292790. thanks to klausman for confirming.
various readability fixes, bug 253058
removed dead link to retired developer's devspace, bug 197814
As announced on the list (http://archives.gentoo.org/gentoo-doc/msg_e721be404c6a5ae8ce5c5bf02f45381c.xml), assume all arches are using the libata framework, so sd* everywhere. includes updating block device and partition descriptions. also added a new included file for boot config (starting sshd, hdparm, etc). synced up several wayward files, including sparc. also changed/dropped usage of some now useless keys, since everyone's using sd*. lots of intensive, invasive changes. and i never even used sed once.
release time. note that since this is beta1, the release dir and stage/media names have been adjusted accordingly. also, the handbooks are marked with a disclaimer=draft, so once the final is out, that will be removed and the release names adjusted. in the mean time, these are live. the beta is officially released. no, it's not april fools, but it is april 1st. :)
updated k3b burning instructions, bug 183651
No universal CD and no networkless install for Alpha/2007.0
Type fix, s/equaly/equally/
2007.0 release is here; final changes and new docs
removing a duplicate paragraph, fixing a typo, thank to grahl for reporting
2006.1 networked docs are in. portage handbook still untouched per separate bugs. thanks to all the hard work, guys. blame me if something is wrong (and please fix quickly) :)
Handbook for 2006.0, "Chuck Norris can divide by zero"
#113849, subkeys.pgp.net instead of pgp.mit.edu
#112511 Chapter 2, minor grammatical error
#105809 - Moving bootstrapping/system to FAQ, outside handbook
#105809 - Stage* description updates
#103724 Fix location of handbook on universal CD
Migrating to 2005.1
#94788 - Fix MILO boot command line
#93503: fixed handbook location on InstallCD
Add stage1/2 con that it requires a working Internet connection during the installation
Updating docos to 2005.0
#81843 - Redundant mentioning of ISO file location
#81843 - Fix typo whish - wish
fix MILO CDROM boot instructions #76918
Separation of Gentoo Handbook into Current and 2004.3
Use GB, MB instead of Gb, Mb... not real standards compliance (not IECs MiB/GiB) but more in line with most users their knowledge. This is "Typo fixing", no version increase
Converted dates to YYYY-MM-DD format
Moving 2004.3 documentation
Adding version/date information in pages
#70077: typo in link (s/#useraccouts/#useraccounts/)
#68422 - Fix useradd command, both in -medium and at the end in the -finalize
Fix english grammer issues
#66814 - Alpha does not have links2, only lynx. Add info on availability of lynx as well (is global)
Use correct su syntax tx to Mark
clarify some of the SRM usage
#59680 - Adding instructions on reading the documentation while installing
Add gpg instructions for checking the validity of the ISOs
#56333:use md5sum.exe to check MD5 on a W machine
Remove digital signature verification as the key used to sign the ISOs has been revoked and the digitale signature files have been removed from the mirrors
#53032 - Move out burning instructions to the Gentoo FAQ. Keep the cdrecord/k3b instructions though
#49376 - Add cryptographic instructions
#50520 - Make it more prominent that stage1 and stage2 cannot be used for networkless installations and that stage3 does
You can only use a stage3 when doing a networkless installation
fix k3b fluency -- it was too confusing
The use of "he/she" and "his/her" isn't recommended. It's ugly and better ways are known such as the use of "they" and "their" (even if it's not plural). Okay, English is a weird language, but I'm learning :) Thanks to Xavier for the information
Dont assume users are masculin
#46548 - Add K3B burning instructions to the burning instructions
#42823 - Separate architecture specific instructions in separate handbooks
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