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Moncton, New Brunswick
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text: Editing Marvel's Daredevil Season 2 3 0 You do not have permission to edit this page, for the following reasons: The action you have requested is limited to users in one of the groups: Autoconfirmed users, FANDOM Staff, FANDOM Helpers, Wiki Managers, Content Team Members. Please log in to edit this wiki.Joining Marvel Database is free, and it only takes a minute.We hope that you sign in, and become a member of the community!Click here to log in or create an account more + You can view and copy the source of this page: {{Marvel Database:Episode Template | Image = | Month = March | Day = 18 | Year = 2016 | Director = Marc Jobst | Producer1_1 = | Writer1_1 = Drew Goddard | PreviousEpisode = Marvel's Daredevil Season 2 2 | NextEpisode = Marvel's Daredevil Season 2 4 | Quotation = | Speaker = | EpisodeTitle = New York's Finest | Synopsis = | Appearing = '''Featured Characters:''' * {{a|[[Matthew Murdock (Earth-199999)|Daredevil (Matt Murdock)]]}} '''Supporting Characters:''' * {{a|[[Nelson and Murdock (Earth-199999)|Nelson and Murdock]]}} ** {{a|[[Karen Page (Earth-199999)|Karen Page]]}} ** {{a|[[Franklin Nelson (Earth-199999)|Foggy Nelson]]}} * {{a|[[Claire Temple (Earth-199999)|Claire Temple]]}} '''Villains:''' * {{a|[[Grotto (Earth-199999)|Grotto]]}} * {{a|[[Frank Castle (Earth-199999)|The Punisher (Frank Castle)]]}} * {{a|[[Dogs of Hell (Earth-199999)|Dogs of Hell]]}} ** President ** Seargent at Arms '''Other Characters:''' * {{a|[[New York City Police Department (Earth-199999)|N.Y.P.D.]]}} ** {{a|[[Blake Tower (Earth-199999)|Attorney Blake Tower]]}} '''Locations:''' * {{a|[[Earth-199999]]}} ** {{a|[[New York City]]}} *** {{a|[[Manhattan]]}} **** {{a|[[Hell's Kitchen]]}} ***** Murdock's Apartment ***** {{a|[[Nelson and Murdock Law Office]]}} ***** Blake's office ***** Dogs of Hell Bar '''Items:''' * {{a|[[Daredevil's Suit]]}} * {{a|[[Daredevil's Billy Club]]}} * {{1st|[[Punisher's Arsenal]]}} '''Vehicles:''' * N/A | Notes = | Trivia = | Recommended = | Links = }} Templates used on the current version of this page: Marvel's Daredevil Season 2 2 (view source) Marvel's Daredevil Season 2 4 (view source) Marvel Database:Episode Template (view source) (protected)Template:! (view source) (protected)Template:1st (view source) Template:A (view source) Template:CloseBrackets (view source) (protected)Template:Death (view source) Template:Deceased (view source) Template:Director1 3 (view source) Template:ENL (view source) Template:Ed (view source) (protected)Template:Extension DPL (view source) (protected)Template:FlashOnly (view source) Template:Flashback (view source) Template:G (view source) Template:GetEraCat (view source) (protected)Template:Green (view source) Template:InfoButton (view source) (protected)Template:Infobox Line (view source) (protected)Template:Infobox Previous-Next (view source) (protected)Template:Mentioned (view source) Template:Month (view source) (protected)Template:Named (view source) Template:OpenBrackets (view source) (protected)Template:QuoteWithCat (view source) (protected)Template:Reflist (view source) (protected)Template:ReleaseDateConverter (view source) (protected)Template:SortLink (view source) Template:Theme.Default.BG (view source) (protected)Template:Theme.Default.Border (view source) (protected)Module:Appearances (view source) Module:HF (view source) (protected)Module:Month (view source) Module:Month/data (view source) Return to Marvel's Daredevil Season 2 3. Retrieved from "https://marvel.fandom.com/wiki/Marvel%27s_Daredevil_Season_2_3" Edit summary Preview Mobile Desktop Show changes

subset: commoncrawl


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—Opening narration
A Long Grudge (Middle of love and Hatred in the Japanese Version) is Paralogue 11 of Fire Emblem Fates.
Paralogue Information[edit | edit source]
This paralogue will be unlocked once Hinata has obtained an S-Support with a female character.
Transcript[edit | edit source]
The script for this paralogue can be found here.
Strategy[edit | edit source]
Subjective: The following part of this article is based upon the editor's personal experiences and opinions, and therefore may not be applicable for all readers.
After passing through a specific chapter, the Faceless encountered are much more stronger, having improved stats in addition to various abilities on them. Hisame is at the center of the map and cannot move. The Faceless nearby don't tend to move so there is plenty of time to reach him, unlike other paralogues where the children can move, often into danger.
The unique aspect in this chapter is the "Waste" tile. Any unit on this tile has their movement reduced to one (excluding flyers and Takumi with his Fujin Yumi), in addition to dropping the unit's avoid rating by 10, so avoid going on waste tiles. There are paths of plain tiles to reach the boss however.
Enemy Reinforcements[edit | edit source]
On turns 7 and 8, 4 Faceless will come out of the upper left and right stairs. On turns 9 and 10, 4 Faceless appear from the lower middle and left stairs.
Triva[edit | edit source]
- If Hisame is defeated but the map is completed successfully, the normal recruitment conversation will occur after the battle, but Hisame will not join the party. The same thing happens in two other paralogues, Two Defenders and Fight or Flight.


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In 1975, Josh Jensen founded Calera in the Gavilan Mountains of California's Central Coast.
Inspired by his mentors in Burgundy, who have grown Pinot Noir in lime-rich soils for centuries;
Jenson's goal was to unearth limestone-rich soils in California to create unique and stylish terroir-driven Pinot Noirs. 40 years later, Calera Wines continues to explore their pioneering spirit, and are revered globally for their elegance, complexity and stylish expression of the Central Coast terroir.


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Jasper Tree
This custom made tree with real tree bark brings life into the home! These are made to order and in-person consults for these trees are required as the trees are built piece by piece around the location in your home it is being made for. Due to this custom process these trees are made in Texas and only currently available to Texas residents. Please contact us at firstname.lastname@example.com for pricing.


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DescriptionChocolate syrup is a great last minute addition to milkshakes, frappes, desserts and ice creams. Unlike chocolate sauce which is thick and gooey, chocolate syrup is thinner in consistency. Commercially made chocolate syrup is derived from the mix of cocoa powder, corn syrup, sugar base, natural or artificial flavourings, and water. You can also make it at home increasing or decreasing the consistency of the syrup as per your taste.How to select• Hershey's is probably the most popular and most often bought store variety of chocolate syrup.• Please do not purchase the syrup if the container shows signs of cracking, spoilage or leakage.• The manufacturing and expiry details must be carefully read before purchasing the product.• Commercially bought syrups contains high amounts of high fructose corn syrup. Please read the label carefully before purchasing.Culinary uses• Adding a shot of syrup can really add flavour and some texture to your basic cup of cold coffee or tea. • Chocolate syrups can be used generously as a topping on pancakes, waffles and slices of cake served with vanilla ice cream.• Mocha lattes are popular caffeine beverages that are a mix of both chocolate syrup and coffee.• Be creative in the kitchen and use the syrup to maximise the taste of beverages and desserts. You can't go wrong with chocolate!How to store• Store chocolate syrups in a refrigerator to maximise its shelf life.• If the chocolate syrup has been made at home, please store it in a coloured bottle so that it doesn't get affected by direct light.
Try Recipes using Chocolate Syrup
Dussehra Recipes
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text: Oxidative Phosphorylation: Electron transfer through the cytochrome system liberating free energy which is transformed into high-energy phosphate bonds.Mitochondria: Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)Uncoupling Agents: Chemical agents that uncouple oxidation from phosphorylation in the metabolic cycle so that ATP synthesis does not occur. Included here are those IONOPHORES that disrupt electron transfer by short-circuiting the proton gradient across mitochondrial membranes.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Oxygen Consumption: The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346)Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Serine: A non-essential amino acid occurring in natural form as the L-isomer. It is synthesized from GLYCINE or THREONINE. It is involved in the biosynthesis of PURINES; PYRIMIDINES; and other amino acids.Glycolysis: A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.Oligomycins: A closely related group of toxic substances elaborated by various strains of Streptomyces. They are 26-membered macrolides with lactone moieties and double bonds and inhibit various ATPases, causing uncoupling of phosphorylation from mitochondrial respiration. Used as tools in cytochemistry. Some specific oligomycins are RUTAMYCIN, peliomycin, and botrycidin (formerly venturicidin X).Mitochondria, Liver: Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)Tyrosine: A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.Cell Respiration: The metabolic process of all living cells (animal and plant) in which oxygen is used to provide a source of energy for the cell.Oxidative Phosphorylation Coupling FactorsProtein-Serine-Threonine Kinases: A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.Mitochondrial Diseases: Diseases caused by abnormal function of the MITOCHONDRIA. They may be caused by mutations, acquired or inherited, in mitochondrial DNA or in nuclear genes that code for mitochondrial components. They may also be the result of acquired mitochondria dysfunction due to adverse effects of drugs, infections, or other environmental causes.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Electron Transport: The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)Dinitrophenols: Organic compounds that contain two nitro groups attached to a phenol.Energy Metabolism: The chemical reactions involved in the production and utilization of various forms of energy in cells.Kinetics: The rate dynamics in chemical or physical systems.PhosphoproteinsProtein Kinases: A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Mitochondrial Proteins: Proteins encoded by the mitochondrial genome or proteins encoded by the nuclear genome that are imported to and resident in the MITOCHONDRIA.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Mitochondria, Muscle: Mitochondria of skeletal and smooth muscle. It does not include myocardial mitochondria for which MITOCHONDRIA, HEART is available.Mitochondria, Heart: The mitochondria of the myocardium.DNA, Mitochondrial: Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.Electron Transport Complex I: A flavoprotein and iron sulfur-containing oxidoreductase complex that catalyzes the conversion of UBIQUINONE to ubiquinol. In MITOCHONDRIA the complex also couples its reaction to the transport of PROTONS across the internal mitochondrial membrane. The NADH DEHYDROGENASE component of the complex can be isolated and is listed as EC 184.108.40.206.2,4-Dinitrophenol: A toxic dye, chemically related to trinitrophenol (picric acid), used in biochemical studies of oxidative processes where it uncouples oxidative phosphorylation. It is also used as a metabolic stimulant. (Stedman, 26th ed)Phosphoserine: The phosphoric acid ester of serine.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Threonine: An essential amino acid occurring naturally in the L-form, which is the active form. It is found in eggs, milk, gelatin, and other proteins.Mitochondrial ADP, ATP Translocases: A class of nucleotide translocases found abundantly in mitochondria that function as integral components of the inner mitochondrial membrane. They facilitate the exchange of ADP and ATP between the cytosol and the mitochondria, thereby linking the subcellular compartments of ATP production to those of ATP utilization.Mitochondrial Proton-Translocating ATPases: Proton-translocating ATPases responsible for ADENOSINE TRIPHOSPHATE synthesis in the MITOCHONDRIA. They derive energy from the respiratory chain-driven reactions that develop high concentrations of protons within the intermembranous space of the mitochondria.Antimycin A: An antibiotic substance produced by Streptomyces species. It inhibits mitochondrial respiration and may deplete cellular levels of ATP. Antimycin A1 has been used as a fungicide, insecticide, and miticide. (From Merck Index, 12th ed)Protein-Tyrosine Kinases: Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.Electron Transport Complex IV: A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Atractyloside: A glycoside of a kaurene type diterpene that is found in some plants including Atractylis gummifera (ATRACTYLIS); COFFEE; XANTHIUM, and CALLILEPIS. Toxicity is due to inhibition of ADENINE NUCLEOTIDE TRANSLOCASE.Succinates: Derivatives of SUCCINIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,4-carboxy terminated aliphatic structure.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Protein Kinase C: An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.Phosphates: Inorganic salts of phosphoric acid.Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone: A proton ionophore that is commonly used as an uncoupling agent in biochemical studies.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Glucose: A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.Rotenone: A botanical insecticide that is an inhibitor of mitochondrial electron transport.Proton-Translocating ATPases: Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.Cyclic AMP-Dependent Protein Kinases: A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Mitochondrial Myopathies: A group of muscle diseases associated with abnormal mitochondria function.Proto-Oncogene Proteins c-akt: A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION. Three isoforms have been described in mammalian cells.PhosphopeptidesPhosphotyrosine: An amino acid that occurs in endogenous proteins. Tyrosine phosphorylation and dephosphorylation plays a role in cellular signal transduction and possibly in cell growth control and carcinogenesis.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.NAD: A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)Reactive Oxygen Species: Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.Carbonyl Cyanide m-Chlorophenyl Hydrazone: A proton ionophore. It is commonly used as an uncoupling agent and inhibitor of photosynthesis because of its effects on mitochondrial and chloroplast membranes.Muscle, Skeletal: A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.Adenine Nucleotide Translocator 1: A subtype of mitochondrial ADP, ATP translocase found primarily in heart muscle (MYOCARDIUM) and skeletal muscle (MUSCLE, SKELETAL).Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Citric Acid Cycle: A series of oxidative reactions in the breakdown of acetyl units derived from GLUCOSE; FATTY ACIDS; or AMINO ACIDS by means of tricarboxylic acid intermediates. The end products are CARBON DIOXIDE, water, and energy in the form of phosphate bonds.Phosphocreatine: An endogenous substance found mainly in skeletal muscle of vertebrates. It has been tried in the treatment of cardiac disorders and has been added to cardioplegic solutions. (Reynolds JEF(Ed): Martindale: The Extra Pharmacopoeia (electronic version). Micromedex, Inc, Englewood, CO, 1996)Polarography: An electrochemical technique for measuring the current that flows in solution as a function of an applied voltage. The observed polarographic wave, resulting from the electrochemical response, depends on the way voltage is applied (linear sweep or differential pulse) and the type of electrode used. Usually a mercury drop electrode is used.Mitogen-Activated Protein Kinases: A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).Cell Line, Tumor: A cell line derived from cultured tumor cells.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Membrane Potential, Mitochondrial: The voltage difference, normally maintained at approximately -180mV, across the INNER MITOCHONDRIAL MEMBRANE, by a net movement of positive charge across the membrane. It is a major component of the PROTON MOTIVE FORCE in MITOCHONDRIA used to drive the synthesis of ATP.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Phosphothreonine: The phosphoric acid ester of threonine. Used as an identifier in the analysis of peptides, proteins, and enzymes.Protein Processing, Post-Translational: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Electron Transport Chain Complex Proteins: A complex of enzymes and PROTON PUMPS located on the inner membrane of the MITOCHONDRIA and in bacterial membranes. The protein complex provides energy in the form of an electrochemical gradient, which may be used by either MITOCHONDRIAL PROTON-TRANSLOCATING ATPASES or BACTERIAL PROTON-TRANSLOCATING ATPASES.Submitochondrial Particles: The various filaments, granules, tubules or other inclusions within mitochondria.MalatesCarrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.ATP Synthetase Complexes: Multisubunit enzyme complexes that synthesize ADENOSINE TRIPHOSPHATE from energy sources such as ions traveling through channels.Peptide Mapping: Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.NADH Dehydrogenase: A flavoprotein and iron sulfur-containing oxidoreductase that catalyzes the oxidation of NADH to NAD. In eukaryotes the enzyme can be found as a component of mitochondrial electron transport complex I. Under experimental conditions the enzyme can use CYTOCHROME C GROUP as the reducing cofactor. The enzyme was formerly listed as EC 22.214.171.124.Casein Kinase II: A ubiquitous casein kinase that is comprised of two distinct catalytic subunits and dimeric regulatory subunit. Casein kinase II has been shown to phosphorylate a large number of substrates, many of which are proteins involved in the regulation of gene expression.Immunoblotting: Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Time Factors: Elements of limited time intervals, contributing to particular results or situations.src-Family Kinases: A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Myocardium: The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Phosphorus Radioisotopes: Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Phosphoprotein Phosphatases: A group of enzymes removing the SERINE- or THREONINE-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992)Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Rats, Wistar: A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.Electron Transport Complex III: A multisubunit enzyme complex that contains CYTOCHROME B GROUP; CYTOCHROME C1; and iron-sulfur centers. It catalyzes the oxidation of ubiquinol to UBIQUINONE, and transfers the electrons to CYTOCHROME C. In MITOCHONDRIA the redox reaction is coupled to the transport of PROTONS across the inner mitochondrial membrane.Mitogen-Activated Protein Kinase 1: A proline-directed serine/threonine protein kinase which mediates signal transduction from the cell surface to the nucleus. Activation of the enzyme by phosphorylation leads to its translocation into the nucleus where it acts upon specific transcription factors. p40 MAPK and p41 MAPK are isoforms.Intracellular Signaling Peptides and Proteins: Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Tetramethylphenylenediamine: Used in the form of the hydrochloride as a reagent in ANALYTICAL CHEMISTRY TECHNIQUES.Protons: Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.Calcium-Calmodulin-Dependent Protein Kinases: A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)Phosphatidylinositol 3-Kinases: Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.Succinic Acid: A water-soluble, colorless crystal with an acid taste that is used as a chemical intermediate, in medicine, the manufacture of lacquers, and to make perfume esters. It is also used in foods as a sequestrant, buffer, and a neutralizing agent. (Hawley's Condensed Chemical Dictionary, 12th ed, p1099; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1851)Glycogen Synthase Kinase 3: A glycogen synthase kinase that was originally described as a key enzyme involved in glycogen metabolism. It regulates a diverse array of functions such as CELL DIVISION, microtubule function and APOPTOSIS.Mitogen-Activated Protein Kinase 3: A 44-kDa extracellular signal-regulated MAP kinase that may play a role the initiation and regulation of MEIOSIS; MITOSIS; and postmitotic functions in differentiated cells. It phosphorylates a number of TRANSCRIPTION FACTORS; and MICROTUBULE-ASSOCIATED PROTEINS.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Mitochondrial Swelling: An increase in MITOCHONDRIAL VOLUME due to an influx of fluid; it occurs in hypotonic solutions due to osmotic pressure and in isotonic solutions as a result of altered permeability of the membranes of respiring mitochondria.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Adenine NucleotidesFibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Pyruvic Acid: An intermediate compound in the metabolism of carbohydrates, proteins, and fats. In thiamine deficiency, its oxidation is retarded and it accumulates in the tissues, especially in nervous structures. (From Stedman, 26th ed)Lactic Acid: A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)Extracellular Signal-Regulated MAP Kinases: A mitogen-activated protein kinase subfamily that is widely expressed and plays a role in regulation of MEIOSIS; MITOSIS; and post mitotic functions in differentiated cells. The extracellular signal regulated MAP kinases are regulated by a broad variety of CELL SURFACE RECEPTORS and can be activated by certain CARCINOGENS.Precipitin Tests: Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Hexokinase: An enzyme that catalyzes the conversion of ATP and a D-hexose to ADP and a D-hexose 6-phosphate. D-Glucose, D-mannose, D-fructose, sorbitol, and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase. (From Enzyme Nomenclature, 1992) EC 184.108.40.206.Amobarbital: A barbiturate with hypnotic and sedative properties (but not antianxiety). Adverse effects are mainly a consequence of dose-related CNS depression and the risk of dependence with continued use is high. (From Martindale, The Extra Pharmacopoeia, 30th ed, p565)Insulin: A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).MAP Kinase Signaling System: An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.Immunoprecipitation: The aggregation of soluble ANTIGENS with ANTIBODIES, alone or with antibody binding factors such as ANTI-ANTIBODIES or STAPHYLOCOCCAL PROTEIN A, into complexes large enough to fall out of solution.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Sodium Cyanide: A highly poisonous compound that is an inhibitor of many metabolic processes and is used as a test reagent for the function of chemoreceptors. It is also used in many industrial processes.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Sodium Azide: A cytochrome oxidase inhibitor which is a nitridizing agent and an inhibitor of terminal oxidation. (From Merck Index, 12th ed)Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Mitochondrial Membranes: The two lipoprotein layers in the MITOCHONDRION. The outer membrane encloses the entire mitochondrion and contains channels with TRANSPORT PROTEINS to move molecules and ions in and out of the organelle. The inner membrane folds into cristae and contains many ENZYMES important to cell METABOLISM and energy production (MITOCHONDRIAL ATP SYNTHASE).Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.Adaptor Proteins, Signal Transducing: A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymesIntracellular Membranes: Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.COS Cells: CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)Azides: Organic or inorganic compounds that contain the -N3 group.Succinate Dehydrogenase: A flavoprotein containing oxidoreductase that catalyzes the dehydrogenation of SUCCINATE to fumarate. In most eukaryotic organisms this enzyme is a component of mitochondrial electron transport complex II.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.p38 Mitogen-Activated Protein Kinases: A mitogen-activated protein kinase subfamily that regulates a variety of cellular processes including CELL GROWTH PROCESSES; CELL DIFFERENTIATION; APOPTOSIS; and cellular responses to INFLAMMATION. The P38 MAP kinases are regulated by CYTOKINE RECEPTORS and can be activated in response to bacterial pathogens.RNA, Small Interfering: Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.Cell Cycle Proteins: Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.Electron Transport Complex II: A flavoprotein oxidase complex that contains iron-sulfur centers. It catalyzes the oxidation of SUCCINATE to fumarate and couples the reaction to the reduction of UBIQUINONE to ubiquinol.Electrophoresis, Gel, Two-Dimensional: Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.AMP-Activated Protein Kinases: Intracellular signaling protein kinases that play a signaling role in the regulation of cellular energy metabolism. Their activity largely depends upon the concentration of cellular AMP which is increased under conditions of low energy or metabolic stress. AMP-activated protein kinases modify enzymes involved in LIPID METABOLISM, which in turn provide substrates needed to convert AMP into ATP.Myosin Light Chains: The smaller subunits of MYOSINS that bind near the head groups of MYOSIN HEAVY CHAINS. The myosin light chains have a molecular weight of about 20 KDa and there are usually one essential and one regulatory pair of light chains associated with each heavy chain. Many myosin light chains that bind calcium are considered "calmodulin-like" proteins.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Tetradecanoylphorbol Acetate: A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.Adenylate Kinase: An enzyme that catalyzes the phosphorylation of AMP to ADP in the presence of ATP or inorganic triphosphate. EC 22.214.171.124.Multienzyme Complexes: Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.Okadaic Acid: A specific inhibitor of phosphoserine/threonine protein phosphatase 1 and 2a. It is also a potent tumor promoter. (Thromb Res 1992;67(4):345-54 & Cancer Res 1993;53(2):239-41)Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).PyruvatesSaccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Membrane Potentials: The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Antimetabolites: Drugs that are chemically similar to naturally occurring metabolites, but differ enough to interfere with normal metabolic pathways. (From AMA Drug Evaluations Annual, 1994, p2033)CDC2 Protein Kinase: Phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the CELL CYCLE. It is the catalytic subunit of the MATURATION-PROMOTING FACTOR and complexes with both CYCLIN A and CYCLIN B in mammalian cells. The maximal activity of cyclin-dependent kinase 1 is achieved when it is fully dephosphorylated.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Arsenates: Inorganic or organic salts and esters of arsenic acid.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Dicyclohexylcarbodiimide: A carbodiimide that is used as a chemical intermediate and coupling agent in peptide synthesis. (From Hawley's Condensed Chemical Dictionary, 12th ed)Valinomycin: A cyclododecadepsipeptide ionophore antibiotic produced by Streptomyces fulvissimus and related to the enniatins. It is composed of 3 moles each of L-valine, D-alpha-hydroxyisovaleric acid, D-valine, and L-lactic acid linked alternately to form a 36-membered ring. (From Merck Index, 11th ed) Valinomycin is a potassium selective ionophore and is commonly used as a tool in biochemical studies.Amino Acid Substitution: The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.Protein Kinase Inhibitors: Agents that inhibit PROTEIN KINASES.Trityl CompoundsMice, Inbred C57BLPotassium Cyanide: A highly poisonous compound that is an inhibitor of many metabolic processes, but has been shown to be an especially potent inhibitor of heme enzymes and hemeproteins. It is used in many industrial processes.Antibodies, Phospho-Specific: Antibodies directed against immunogen-coupled phosphorylated PEPTIDES corresponding to amino acids surrounding the PHOSPHORYLATION site. They are used to study proteins involved in SIGNAL TRANSDUCTION pathways. (From Methods Mol Biol 2000; 99:177-89)Protein Tyrosine Phosphatases: An enzyme group that specifically dephosphorylates phosphotyrosyl residues in selected proteins. Together with PROTEIN-TYROSINE KINASE, it regulates tyrosine phosphorylation and dephosphorylation in cellular signal transduction and may play a role in cell growth control and carcinogenesis.Focal Adhesion Protein-Tyrosine Kinases: A family of non-receptor, PROLINE-rich protein-tyrosine kinases.Ribosomal Protein S6 Kinases, 90-kDa: A family of ribosomal protein S6 kinases that are structurally distinguished from RIBOSOMAL PROTEIN S6 KINASES, 70-KDA by their apparent molecular size and the fact they contain two functional kinase domains. Although considered RIBOSOMAL PROTEIN S6 KINASES, members of this family are activated via the MAP KINASE SIGNALING SYSTEM and have been shown to act on a diverse array of substrates that are involved in cellular regulation such as RIBOSOMAL PROTEIN S6 and CAMP RESPONSE ELEMENT-BINDING PROTEIN.3T3 Cells: Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.14-3-3 Proteins: A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Proton Pumps: Integral membrane proteins that transport protons across a membrane. This transport can be linked to the hydrolysis of ADENOSINE TRIPHOSPHATE. What is referred to as proton pump inhibitors frequently is about POTASSIUM HYDROGEN ATPASE.Paxillin: Paxillin is a signal transducing adaptor protein that localizes to FOCAL ADHESIONS via its four LIM domains. It undergoes PHOSPHORYLATION in response to integrin-mediated CELL ADHESION, and interacts with a variety of proteins including VINCULIN; FOCAL ADHESION KINASE; PROTO-ONCOGENE PROTEIN PP60(C-SRC); and PROTO-ONCOGENE PROTEIN C-CRK.Vanadates: Oxyvanadium ions in various states of oxidation. They act primarily as ion transport inhibitors due to their inhibition of Na(+)-, K(+)-, and Ca(+)-ATPase transport systems. They also have insulin-like action, positive inotropic action on cardiac ventricular muscle, and other metabolic effects.Protein Phosphatase 2: A phosphoprotein phosphatase subtype that is comprised of a catalytic subunit and two different regulatory subunits. At least two genes encode isoforms of the protein phosphatase catalytic subunit, while several isoforms of regulatory subunits exist due to the presence of multiple genes and the alternative splicing of their mRNAs. Protein phosphatase 2 acts on a broad variety of cellular proteins and may play a role as a regulator of intracellular signaling processes.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Focal Adhesion Kinase 1: A non-receptor protein tyrosine kinase that is localized to FOCAL ADHESIONS and is a central component of integrin-mediated SIGNAL TRANSDUCTION PATHWAYS. Focal adhesion kinase 1 interacts with PAXILLIN and undergoes PHOSPHORYLATION in response to adhesion of cell surface integrins to the EXTRACELLULAR MATRIX. Phosphorylated p125FAK protein binds to a variety of SH2 DOMAIN and SH3 DOMAIN containing proteins and helps regulate CELL ADHESION and CELL MIGRATION.


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Electric scooter startups are about to run into Trump tariffs
Karen Graham
Aug 19, 2018 in
As electric scooter-rental startups like Bird and Lime battle for market share on American streets, in a matter of days, the budding companies will be slammed with a slew of Trump tariffs.
Xiaomi, the so-called Apple of China, has emerged as a
leading supplier of scooters
for Silicon Valley transit startups. The rebranded Xiaomi scooters have been deployed across US cities by scooter startup, Bird, which in May locked up a deal with the company worth tens of millions of dollars to become one of its vendors.
Xiaomi scooters are also used by Bird competitor, Lime. The other major scooter-maker is
Beijing-based Segway/Ninebot
(it supplies for Lime), which has a close relationship with Xiaomi and counts it as a backer. So, seeing as most US e-scooter companies rely on vehicles manufactured in China, the coming slew of tariffs will hit the two-wheeled revolution hard.
An electric scooter belonging to the startup Bird waits for a rider on a street of downtown Washington, DC
Eric BARADAT, AFP
The coming tariffs, announced on August 7, are round-two of the taxes that the US has imposed on Chinese goods. Going into effect on August 23, a 25 percent tariff on $16 billion in Chinese goods will undoubtedly bring the simmering trade war with China to a boil.
On the
proposed list of affected goods,
hidden between special purpose motor vehicles and refrigerated vessels, is HTS code 8711.60.00, described as "Motorcycles (incl. mopeds) and cycles, w/electric motor for propulsion." In other words, electric bicycles and scooters.
E-scooters already have a lot to deal with
The electric scooter craze has taken hold of cities around the world, and since coming to San Francisco last March, the scooters have literally swarmed across the United States, popping up in
over 20 cities
, from Miami, Florida to Salt Lake City, Utah.
File photo: Segway scooter.
Ferienagentur (CC BY-SA 3.0)
The scooters are dockless and are touted as a way to be more environmentally friendly, and ease gridlock while letting riders zip through streets. "Ride your way," the San Francisco-based Spin promises. Even Uber and Lyft got into the growing market.
And here is where things began to get a bit sticky. The competition grew to the point that instead of asking cities if they would like to try out this revolutionary way of getting about crowded downtown streets - they began dumping the scooters on the unsuspecting cities.
One of the most recent dumps happened in Richmond, Virginia, where Segway has been used for a number of years. On Thursday last week, Santa Monica, California-based Bird dropped a batch of its vehicles in the city in a blitz that quickly resulted in riders zipping around town.
By Thursday afternoon, according to
Richmond BizSense,
all the scooters had been impounded. Tom Byrnes, a spokesman for the mayor's office, confirmed that the city was collecting and impounding the scooters, saying that Bird did not have the permissions needed to drop their fleet in the city. "They deployed them without engaging the city. We had no contact with them," Byrnes said.
Scooter vs. car on Toronto's Queen Street East Tuesday during the lunch hour backed up traffic.
KJ Mullins
No regulation nationwide
Scooter companies have started a pattern - dump the scooters, watch the demand for them rise, and then sit back and wait for a city to give-in. But cities are starting to fight back.
Just as Richmond has done, other cities are issuing cease and desist orders, coupled with city-wide bans, and a torrent of criticism from local governments and citizen groups. But even with this latest problem within the country, it is still the tariffs that may be the scooter's undoing.
Over the last six months, according to
PIERS import data,
China imported into the U.S. almost $61 million worth of goods under the e-scooter description. A 25 percent tariff would have raised costs for scooter importers by over $15 million.
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Local pilots and work colleagues achieve the next step at Devon & Somerset Gliding Club
PUBLISHED: 12:43 10 July 2018 | UPDATED: 12:43 10 July 2018
With a super week of weather for gliding at North Hill, the July course enjoyed flying in blazing sunshine on four of the five days – Wednesday being the only day that the course was rained off.
However, the latter poor weather enabled course members to catch up on some theory with a series of briefings from instructors Stuart Procter and Martin Woolner.
Congratulations to friends and work colleagues Dave Herbert from Exmouth and Lukasz Kieruczenko from Exeter, who both achieved their Bronze level during the week, and solo aerotow.
Unity Five Limited is a software development company formed in 2010 by Dave Herbert and Lukasz Kieruczenko, specialising in web and mobile applications. Currently their software products are used across the UK and Republic of Ireland. The company is rapidly expanding with 27 employees and is just about to enter the US market.
Lukasz Kieruczenko said: "We didn't stop after going solo and both achieved over 20 hours as pilots in command. It's been an amazing journey and all the instructors at DSGC were extremely helpful. We learn something new with every flight. Our next goal is to get a cross-country endorsement and buy our own glider."
Try gliding by starting with a trial lesson. Find out about trial lessons at Devon & Somerset Gliding Club and purchase and book online by visiting their website, dsgc.co.uk
If you value what this story gives you, please consider supporting the Midweek Herald. Click the link in the orange box above for details.


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This Acacia Honey hails from the glorious and pristine highlands of Hungary, just south of the Austrian boarder. The clean, light vanilla taste is perfection on the palate. This single-varietal gem is made from the delicate blossoms of the Acacia tree. One taste of this fine honey, and you will be an Acacia lover forever! This is honey perfection. Acacia Honey is very rare and dependent on a variety of climatic conditions. As always, our supply is limited and will not last very long. Sometimes folks refer to Acacia honey as Moonflower honey or Water honey.


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Over 25 years experience providing everything for baby!
Palm moses basket with soft waffle drapes in Vanilla. Includes mattress. Stand sold separately.
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Team Member
Will Jeffels
Clinical Director Small Animals
Will is a Clinical Director in the practice. His interests include feline medicine, cardiology and ultrasound. Will has three dogs, Bolly and Tatty the mad Springer Spaniels and Poppy, a Norfolk Terrier, two cats, two horses (which he claims no responsibility for), ducks and chickens!


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Specifications: * Automatic Lester 48-volt charger Model #19300 with PowerWise DC plug* Fits E-Z-GO Electric 48-volt Industrial vehicles 1999-Up* Replaces OEM Number(s): 20330-98
Specifications: * Charger Plug for E-Z-GO 48-Volt Powerwise Chargers* Comes with everything shown
…from 0 to100% in large easy-to-read format* Environment protection of IP65 (with connector installed)ensures operation in harsh environments Includes: * Solid State Battery Meter* 48 Volt Wire Harness Please Note: Will not work with E-Z-GO RXV models. Please use #8062 for all RXV models.
Specifications: * 48-Volt Battery Charger* Fits E-Z-GO Electric 48-volt 2008-Up RXV Replaces OEM Number(s): * 60289* 607468* 611200* 622416* 622418
Specifications: * Charger receptacle for 48-volt Delta Q charger* Fits E-Z-GO electric 2010-up TXT* Replaces OEM Number(s): 613304
Specifications: * 48-volt charger receptacle* Fits E-Z-GO RXV 2008-Up* Replaces OEM Number(s): 602529
…for all battery types.* Use it with any type wet cell lead acid batteries.* Automatic three-stage charger can be left on the batteries indefinitely.Note: This product is not a charger. It is intended to maintain the level of charge in your batteries only. Click here for Buggies Unlimited Chargers.
…performance of new and older batteries considerably longer that standard chargers, ensuring that your batteries stay fully charged and free of sulphate for months at a time, whether operational or unattended. Designed for all 48 volt systems, including electric golf cars, people movers, fork and…


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Aug 19, 2007 Of Rocky, Joe Jackson, and Muzak
Image from the public domain.
Many times I have blogged (e.g., here and here) that great music and musicians inspire me as a trial lawyer. I also have cursed the day Muzak was born, more specifically elevator music, now that Muzak has branched out beyond the elevator.
Recently, I got almost so sick to my stomach with elevator music, that I would have gotten off and climbed the stairs — or else abandoned the building — had I not merely been going to the seventh floor. My Sunday morning started off in Philadelphia, where I was spending a weekend. I finally got a chance to walk right up to the Philadelphia Museum of Art, where Rocky ran up its steps in the first of the Rocky series, followed by drinking a glass of six raw eggs. I could not pass up a chance to run up the same steps,. Earlier this year, I went to an even more exciting part of Philadelphia, just a few blocks further away, to an otherwise ordinary-looking residential block where John Coltrane's home sits.
When I got back to my hotel a few blocks away and hit the button to my floor, the doors closed on me like a jail cell, and I almost lost my breakfast, as the speakers piped in an elevator music version of Joe Jackson's "Is She Really Going Out With Him?" I was particularly surprised that Joe Jackson would not have protected all his music from such putrefication, although my free expression zealousness has no problem that he did not (but my stomach does have such a problem). In any event, when I saw Joe Jackson perform an amazing concert in 1983 at Miami's Knight Center, he seemed too serious about his music to tolerate its being turned into elevator music. In fact, at one point during the concert, he briefly lectured the audience, questioning why people would pay good money for the concert, only to shorten his total performance time on stage with a bunch of "woo woo"-ing.
This elevator music torture was perhaps a payback for my doing something as hackneyed as running the Rocky steps. Perhaps it also was payback for the time I strolled upon a storefront Muzak office around seventeen years ago during lunchtime in Washington, DC, and asked for some information about the company. Perhaps sensing my mischievous intent, the person at the front desk introduced me to the manager and general counsel, who inquired whether my interest in Muzak was morbid. I replied that Muzak is here to stay, for better or worse, so I wanted to know more about it. He handed me a brochure and a major magazine article about Muzak's efforts to remake its image beyond elevator music.
Of course, elevator music is far from my only musical pet peeve. Another is Phil Collins, no matter his talent and tremendous popularity. About a decade ago, I made the mistake of turning on the television one weekend morning to see Phil Collins being interviewed. He seemed to have a very dry mouth, the same kind I experienced — accompanied by an extremely queasy stomach — several Sunday mornings in college after drinking too much the night before. Forever after that, my stomach starts feeling queasy when I hear Phil Collins, making me feel worse than I felt hearing Joe Jackson transformed into elevator music.
The only link I can make between these queasy ordeals on the one hand and trial advocacy on the other, is the necessity to be tolerant and patient in preparing for and pursuing trials, dealing with clients, and dealing with all people. Absent such tolerance and patience, a lawyer will be rolling his or her eyes over testimony and evidence that at first blush seems painfully boring or annoying, but which might also contain some gold if only the lawyer would pay attention. Does that mean I should stop switching the station every time Phil Collins comes on? Eating a crate of prunes sounds less unpleasant than listening to Phil Collins. Jon Katz.


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Diageo, the maker of Johnnie Walker whiskey, found an innovative way to plug a gaping deficit in its pension plan: put aside 2 million barrels of maturing whiskey from its distilleries in Scotland.
Diageo said Thursday it would transfer ownership of £430 million, or $645 million, worth of whiskey to a pension funding partnership. Diageo employees would not receive their pensions in whiskey rather than cash, but the move does give them a guarantee that they would not walk away empty-handed should the company default.


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Human Growth Hormone; All About HGH In Bodybuilding
Since the first application of the HGH in bodybuilding, it has been met with a lot of criticism and has attracted conflicting opinions. In the quest for greater muscle mass and a ripped look, bodybuilders have resulted in many supplements, pharmaceutical and non-pharmaceutical, to achieve their goals. HGH is one of them.
What is HGH?
This hormone is created in the pituitary organ, and just like its name implies, the growth hormone is accountable for cell renewal and growth. Bone density and muscle mass increase are not possible without the growth hormone.
After secretion, the GH is only active for a couple of minutes which is not enough time for the liver to convert it to growth factor.
How much HGH does an average human being produce naturally?
Healthy adult males have less than five nanograms per millimeter of GH circulating in their blood. Healthy adult females produce double the amount because of child-bearing. The level of the GH rises rapidly in puberty but experiences a sharp drop in the early 20s.
How is HGH deficiency diagnosed?
An HGH test can be performed by your doctor to determine the levels.
Is too much GH dangerous?
Medical researchers agree that the media overblow the dangers of the GH. Complications with use are minimal. Cases of a significant liver and big feet are only experienced in patients who use the supplement in mega doses.
he impact of HGH in bodybuilders
This hormone leads to:
Following the injection of this hormone in the body, it enters the bloodstream and is distributed all over the body. Higher concentrations are in the liver where it is converted to growth factors. It is responsible for the growth of muscles which is the primary reason why bodybuilders use it.
The hormone itself performs the fat-burning effect of the GH. It binds to receptors that elevate the level of free fatty acids. Consequently, the body will use fats as a source of energy instead of carbohydrates.
Loss of fat does not affect muscle growth. That is why the growth hormone is preferred for people who want to lose fat without interfering with the lean muscle mass. This hormone leads to hyperplasia ( increase in muscle cells as a result of proliferation). This effect is desirable because the muscle gain is permanent.
How to choose HGH brand
Bodybuilders require this hormone in large quantities for more read Human Growth Hormone review. It is advisable to go for a cheaper, high-quality brand. As long as the brand has been confirmed to give excellent results, you should not hesitate. Going to expensive brands will prove to be costly in the long run.
Many people seem to think that steroids and HGH are the same things. While mostly they seem to deliver the same results, they work very differently. Steroids encourage the production of testosterone which is a male sex hormone. Testosterone is produced in the testes of a man. These are known as anabolic steroids, and they encourage muscle growth as well. They also help to burn fat. HGH, on the other hand, is not a sex hormone and is not produced by the testes, but the pituitary gland instead. It encourages and triggers the growth of body tissue, which is why it is useful when it comes to repairing damaged tissue in the body. So it speeds up recovery time. HGH has various benefits. It helps you grow more tissue and may even reverse or slow down the signs of aging.
While both of these supplements have their advantages, they also have their side effects. You must be prepared to accept the good and the bad. The adverse effects of steroids are that it can cause the body to stop producing testosterone naturally, leading to an over-production of estrogen. In turn, it leads to the enlargement of breasts in men and hair loss, acne, and so on. Alternatively, HGH has side effects too. It can cause the body to continue growing at a phenomenal rate. Certain features on the face like the chin and nose could continue growing until it no longer looks natural.
So while both of these supplements deliver exceptional results, you could also be exposed to the adverse effects. Certain side outcomes do not affect all users, and you may experience any of them. However, you should be aware of them, just in case you do experience them after using steroids or HGH. The onus is on yourself to do your investigation and ensure that you are healthy enough to take either one of these supplements.
Irrespective of which supplement you choose to go with, remember that neither one of them should be taken continuously with a break. You should provide at least one to two weeks off the supplements before going back on them for a period of roughly four to six weeks. There are no short cuts in training or bodybuilding, and even if you are taking supplements, you still need to put actual effort into it. You need to have a balanced diet, an exercise regime, and lots of discipline to reach your bodybuilding goals. Succumbing to fast food and days off are incredibly tempting. However, if you give in, you will never see any good results. So it may be tough at first, but try your best to stick to your plan.


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By: RH
Go Daddy and NameJet Daily Auction Spotlight 8/7/12
Go Daddy Auctions
BusinessArchitechts.com 8 year old domain. I like this name for a company name.
Redsy.com 5 letter pronounceable .com
CafeCash.com affiliate program name maybe ?
sunnydale.net for fans of the show Buffy the Vampire Slayer.
Go Daddy Closeouts
CasinoJobs.us 8 year old domain. Not bad name for $5 exact search volume for US at 2400.
Esorority.com.com 13 year old domain, online sorority site for social media, maybe provide tech service to local chapters.
Penbot.com 8 year old domain, tech product name.
FairfaxCondos.com 10 year old domain. Virginia Geo.
Drama-TV.com 13 year old domain. Use for a blog or community site. These type of sites do better than you think from building traffic, but advertisers don't pay that much if you are using Adsense.
MysticGames.net 10 year old domain, Go Daddy estimates a little traffic.
Rediscovered.net 8 year old domain. One word .net, that is not commercial but some just like one word domains.
NameJet under the radar picks (Back orders must be placed by 11p.m. Eastern Time)
Not a lot of names today that I like.
ColoradoCreditUnions.com create a directory or blog, advertising CPC is pretty good.
OnlineForexTools.com Another good paying keyword, could be flipped.
Full Disclosure: Affiliate links, publisher receives 10 % commission on successful bid.


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Trivia Question
Which Major League baseball team has its Spring Training home located in closest proximity to its regular-season home stadium?
Trivia Question Answer
The Arizona Diamond backs hold spring training at Salt River Fields at Talking Stick which is only 18.8 miles from their home field – Chase Stadium. Fun fact about the Salt River Fields at Talking Stick is that it was the stadium to be built on Indian land. Second closest are the Tampa Bay Rays who hold spring training at the Charlotte Sports Park only 79.4 miles from Tropicana Field where they play their regular season home games (according to Google Maps). This is only 3.2 miles better than the Florida Marlins who hold spring training in Roger Dean Stadium 82.6 miles away from their home field Marlins Park.


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About us
Xpublisher was founded in 2009 and is headquartered in Munich, Germany. The company offers intuitive content solutions and specializes in standardizing, semantically tagging and publishing content. The company's flagship products Xpublisher and Xeditor simplify the complexity of creating structured content and provide non-technical users with the best possible editing and content management experience. Xpublisher operates a subsidiary in the USA. Numerous international companies and organizations from industry, aerospace, publishing and government rely on Xpublisher's products.
Who We are and How We Work
We are an international team of diverse nationalities, personalities and interests, but we all have a common denominator: we are passionate about innovative technologies and strive towards the best solutions for our customers! This is sometimes a real challenge, as we work in a fast-paced industry and constantly need to question what we do. We have acquired our know-how by working together closely with customers from a wide range of sectors. And we'll stay the course, in order to continue developing alongside our customers. This is what makes our inspiring environment so special!
What else distinguishes us?
We spend a lot of time together and it is important to us that we feel at ease here. We foster an informal atmosphere, respectful interactions, and last but not least, the chances of having a lot of fun. We always find a good occasion for that: whether a white sausage breakfast, a sundowner, or a St. Nicholas brunch. We don't just want to be an employer. We'd like to offer more than that.


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by Eric Stashin (aka The Rotoprofessor)
It seemed like 2018 was a coming out party for the Mariners' Marco Gonzales, a former St. Louis Cardinals' first round draft pick. Despite that pedigree he hadn't gotten much attention, though he clearly opened some eyes with these numbers:
13 Wins
145 Strikeouts (7.83 K/9)
32 Walks (1.73 BB/9)
44.9% Groundball Rate
It's obvious what his carrying tool is, as he used elite control to keep himself productive. That said with a minor league career 2.3 BB/9, including a 2.5 at Triple-A, you have to wonder how maintainable that number is. Even a small regression could spell doom, unless there's upside in his strikeouts and/or groundball rate.
In terms of strikeouts, it's not a skill he's ever shown before (7.9 K/9 over his minor league career). Having averaged 90.89 mph on his fourseam fastball and 90.81 on his sinker, it's obvious that he's not an overpowering pitcher. He also doesn't feature a true wipeout pitch, which would help us to anticipate an improvement in his strikeout rate (Whiff%):
- Changeup – 12.39%
- Cutter – 11.78%
- Curveball – 11.56%
The cutter is a new pitch for him, having not thrown it in his previously limited time in the Majors, though he utilized it 22.21% of the time. He was productive with it (.224 BAA, .397 SLG), but it's development doesn't offer upside in strikeouts. If he's not going to overpower hitters and he doesn't appear to have deceiving stuff, why would we expect an improvement?
Maybe he's still working his way back from Tommy John surgery, which cost him virtually all of 2016, but he's now had 293.0 innings since returning to the mound. If that was the issue we'd have expected to have seen signs of it already.
As for the groundball rate, a 1.06 GO/AO over his minor league career tells you that it's never going to be a strength. Pitching in Seattle may help to limit the risk of home runs, but there's certainly room for a regression to his 0.92 HR/9. Just consider this split:
- Home – 0.45
- Road – 1.34
With there being a good chance that the home runs mount at home, even if it's just up to the 0.80 range (though it could go higher than that), the risk continues to grow. While his Hard% wasn't outrageous (34.0%), it appeared that when hitters squared him up they were on a line (25.1% line drive rate). That brings risk to maintaining a strong BABIP, with last year's mark being a believable expectation.
You put it all together and you get:
- A pedestrian strikeout rate
- Risk of significantly more home runs
- Control that could regress, even just a little bit
- Potential to carry a bloated BABIP
None of that sounds promising, and while there may be hype surrounding him entering the season he's simply not a pitcher you want to target.
Sources – Fangraphs, Brooks Baseball, MILB.com, Baseball Reference
Pre-order Rotoprofessor's 2018 Fantasy Baseball Draft Guide for just $6.75!! Click here for the details, but don't miss out on the best bargain in fantasy baseball preparation.
Make sure to check out all of our 2019 projections:
Date Posted
|Dylan Bundy
|Foltynewicz, Mike
|Gibson, Kyle
|Hoskins, Rhys
|Hosmer, Eric
|Wheeler, Zack


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Kefalonia by Anna suggests…
Sea Rock ws Restaurant
Come here to relax, enjoy calming sea views or even admire the moon rising! At Sea Rock ως you can stop for a coffee, drink or a sweet, or even better have a proper lunch or dinner, select from a variety of Greek dishes and a good selection of wines.
Nikos, the owner and the staff are very friendly, the atmosphere of the place is relaxing and the food is excellent. The restaurant has a beautiful garden with amazing sea views. The low light and well selected music together with the simplicity of the decoration, make for a relaxing setting.
Located at the coastal road of Agia Efimia 800m from the busy center of Agia Efimia, at the best part of it!
While enjoying your meal, you can watch the boats coming in and out from the harbour and and if you are lucky to be here close to the full moon it is even better!
They offer a great Taste of Kefalonia Food Experience too!
Contact Info
Please mention Kefalonia By Anna when you visit Sea Rock ws to get your treat!
Sea Rock ws
Agia Efimia, 28081 Kefalonia
+30 6909 919 534


text: COSMOS+TAXIS takes its inspiration from Friedrich Hayek's (1979) distinction between spontaneous and planned orders. A spontaneous order, as Gus diZerega explains in the open- ing article, evolves in an orderly but unplanned fashion be- cause of the presence of shared rules, simplified feedback, and status equality among the order's participants. Though the most studied instantiation is the market order, this jour- nal is concerned with the broad manifold of spontaneous orders. Michael Polanyi (1962) used the term "spontaneous order" to explicate the evolution of science, while diZerega (1989) extended its application to democracy as distin- guished from the traditional authoritarian nation state, the largest-scale example of a taxis.


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Hotel Capri is set in the town of Bardolino, a 2-minute walk from Lake Garda. It offers a swimming pool surrounded by palm trees, estacionamiento gratis, and rooms with balconies. You room features air conditioning, televisión satelital, y minibar. Parking is free both at the open-air car park and a garage, 250 m. The Capri Hotel is ideally located for boat trips across the lake and cycling along the lake's shores. Nearby golf courses include Cà degli Ulivi and Golf Paradiso, 15 y 20 minutes' drive away respectively.
Servicios e instalaciones


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Joseph Biden, the 65-year-old Delaware senator has, by all accounts, a superlative resumé.
Biden's leadership of the foreign relations committee is expected to benefit Democrats during a presidential race that could be decided as much by Iran, Iraq and Russia as by the US.
His son is scheduled for a deployment to Iraq this year, and he has a palpable affection for Barack Obama.
Biden, first elected at the age of 30, is a demonstrably skilled debater. Washington has produced few sparkling wits, but he is one of them.
During a Democratic presidential debate in February, the famously talkative Pennsylvania native was asked whether he had the self-discipline to lead the free world.
Most expected one of his long-winded answers, but he shocked the crowd into laughter by answering: "Yes."
And he offered one of the most succinct putdowns of the campaign when he said of Rudy Giuliani: "There's only three things he mentions in a sentence - a noun, a verb and 9/11."
Biden's drawbacks, however, go beyond his verbosity. His presidential candidacy in 1988 derailed after he admitted to plagiarising a speech by the then British Labour party leader, Neil Kinnock.
His long history in Washington also risks undermining Obama's message of "change".
Biden, who dropped out of the presidential race in Iowa in January, is of Irish Catholic heritage but is in favour of abortion rights, a key issue in US elections.
He originally voted to authorise the war in Iraq, but has since become a persistent critic of the Bush administration's policies there.
Born into a working class family in Scranton, Pennsylvania on November 20 1942, Biden is not a household name but was arguably the most well-known of those being considered for the vice presidency by Obama.
He was the chairman of the Senate judiciary committee during two of the most contentious supreme court nomination battles of the past 50 years.
He led the opposition to the nominations of Robert H Bork, who was defeated, and Clarence Thomas, who was later confirmed.
Biden married Neilia Hunter in 1966, and the couple had three children. His wife and 13-month-old daughter. Naomi. died in a car accident in December 1972, shortly after he was first elected to the Senate.
His two sons, Joseph R Biden III and Robert Hunter, were seriously injured, but Biden was persuaded not to resign to look after them and was sworn into office from their bedside. Both made full recoveries.
In 1977, he married Jill Tracy Jacobs, with whom he has a daughter, Ashley.
Biden is currently serving his sixth term as a member of the Democratic Party. He has served for the sixth-longest period among current senators, and is Delaware's longest-serving senator.


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Multicultural Shopping & Dining
Interested in trying one of the many multicultural grocery stores or restaurants in our region? Click here to download the most updated list (updated 10/04/19)! And if you see that your favorite restaurant is missing, contact us to let us know!
The Dayton community has a diverse and extensive support network of resources, services and agencies in place to help our immigrant population. Local businesses, institutions and schools offer an assortment of programs and services to assist in day-to-day interactions between foreign-born and native-born residents. Many may not be aware of the resources already in place, so we've put together the links to the right to help educate immigrants as well as local businesses/organizations about the services already available.
For those wishing to add their programs to our list, please contact us with information about your program, service or other immigrant resource.
We have the highest number of immigrants living in this area than ever before. Become a Welcome Dayton Ambassador and help us make Dayton even more immigrant-friendly!


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Josiah Wolf Jet Lag (new)
Published Mar 01, 2010Josiah Wolf (drummer of WHY?) is back to his solo glory, bringing us a fantastic album. But it comes with some wary notes; Wolf made Jet Lag after the end of an 11-year marriage. Best described lyrically in "The Apart Meant," he sings: "My apartment smells like divorce, unused 'I love yous' build up in my throat." Wolf laments the past, but does seem almost ready for the future. Besides his uncanny resemblance to the Unicorns/Islands, musically, Wolf's voice is relaxed but capable of a wide range. He creates a hopeful and sometimes psychedelic atmosphere with bells, organs, kalimbas, guitars, drums, bass and more. Wolf is perfectly ordinary, singing about a new car smell and finding change. It's endearing and inspiring that this sombre album has a more playful side. It feels like your best friend paying you back with a favour for giving them a really great hug. (Anticon)


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Major Retailers Offer PlayStation VR Discount
Gamers hoping to try out the Virtual Reality (VR) bandwagon should take advantage of the latest deal from Sony.
by Michael Augustin
PlayStation VR: Sony Shares Sales Numbers
Among the available VR headsets currently available in the market, Sony is surprisingly right on top.
by Michael Augustin


text: The aim of this research was to investigate whether there is a correlation between the use of social media by adolescents and the social self-esteem of adolescents. In addition, checks whether this association can be explained by the extent of social competence in adolescents. A positive correlation was expected between ... read more


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Champions League Last-16 Draw: Liverpool, Tottenham & Man Utd Given Tough Tasks
Liverpool, Man Utd, Tottenham and Man City have discovered their fate after the draw for the last-16 of the Champions League was conducted this morning.
Liverpool, Manchester United, Tottenham and Manchester City have discovered their fate after the draw for the last-16 of the Champions League was conducted this morning.
Liverpool booked their place in the last-16 thanks to a dramatic 1-0 victory over Napoli at Anfield last week. The win secured second place in Group C behind Paris Saint-Germain and Jurgen Klopp's men have now been drawn to face German giants Bayern Munich.
Manchester United also finished second in their group with the Red Devils having to settle for the runners-up spot behind Juventus in Group H after losing their final game to Valencia last week. Jose Mouriho's side have now been drawn to face PSG in the next stage of the competition and it will be a big task for United to reach the quarter finals.
Manchester City made light work of the group stages of the Champions League as the Premier League champions finished five points clear of Lyon at the top Group F and they've been rewarded by getting Schalke in the last-16. Pep Guardiola will be pleased with that draw and no doubt City will be the red hot favourites to progress.
Tottenham ensured all four English sides made it through to the knockout stages after drawing 1-1 at Barcelona last week and with Inter Milan failing to beat PSV at the San Siro, Mauricio Pochettino's men snatched second place. The north Londoners have been drawn to face Bundesliga leaders Borussia Dortmund in the last-16 and it's sure to be an exciting double-header between two attacking sides.
It's fair to say we have some huge matches to look forward to in February and March next year. Liverpool, Spurs and United have all got difficult tasks ahead of them but City should breeze in to the last-8 of the Champions League.
Here is the full draw:
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Daughter a liability for SC woman-judge
At a time when Indian woman are breaking the glass ceiling and rocking the board room with their dynamism, this comes as a put-off. The SC has made it mandatory to declare the assets and liabilities of its judges. This is where Justice Mishra has declared "two daughters to be married" in the liabilities column.
What can be considered a popular sentiment of the middle class for whom the family budget is a tight rope walk, what is surprising is that this sentiment was revealed by India's senior woman judge. With the governments widespread campaigns to put forth that the girl-child is never a burden, this news has come as a eye-opener for the apparent "learned" ones amongst us. The other entries in the judge's liabilities column include "guarantor for education loan of my daughter" and residential house to be built post-retirement.
The declaration will raise quite a few eyebrows since it came from a key representative of the apex body. The issue gains prominence as the SC is called-in to give judgment is various gender issues and is supposed to give an impartial opinion on women's rights and other social issues.
In the asset section, Justice Mishra who was with the Chhattisgarh high court previously declared that "Stridhan" of one gold set and "few gold rings and a pair of bangles." The term "stridhan" garners various interpretations with its literal meaning.
While one would want to believe that 'sthree' (woman) is the real wealth rather than what she brings from her family that is precious. Hope good sense prevails among the educated and well-endowed amongst us and be able to set good examples among the less fortunate, both in terms of sense and wealth.
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