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Privacy Policy
PRIVACY POLICY FOR SilkKnittingYarn.com
Our Company is dedicated to protecting your privacy when you visit or communicate with our site or personnel. The Privacy Policy offered here, along with our terms of use offers an explanation regarding any personal data you may provide to us or that we garner from you.
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Newbury Classic Style Wooden Captain Bed
Regular price
$ 869.00
This classic twin trundle bed has a built-in bookcase headboard with sliding doors. Mattress ready, this captain bed also includes a trundle and three drawers. Finished in cherry.
- Constructed of solid wood and veneer
- Finish: Cherry
- Bookcase headboard
- Dimensions: 90.25" L x 43.5" W x 49.25" H
- Mattress NOT included
- Bedding (comforter, sheets, pillows, etc.) are NOT included
- Assembly required

subset: commoncrawl


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Your Body & Stress
Let's face it, we can't avoid stress no matter what we do in life. It's part of overcoming obstacles and achieving our goals.
But, when stress becomes unmanageable, it can compromise our health.
The Brain
- Under stress, the hypothalamus signals the ANS and the pituitary gland to release cortisol and epinephrine – the stress hormone.
- Excessive cortisol can interfere with our learning and our memory skills.
- Elevated levels of cortisol may increase the risk of mental illness.
Cardiovascular System
- Stress increases our risk of a heart attack, hypertension and stroke.
- A study in Denmark has shown that pressure from work put people at a higher risk of cardiovascular disease.
- A study in Beijing has shown that, in women only, job strain increased the thickness of the carotid artery – which is one of the early signs of heart disease.
- Stress can cause increased muscle tension.
- Chronic musculo-skeletal pain.
- Tension headaches.
- Stress can even cause muscle tension that is so severe, it would be enough to lead to atrophy. Atrophy is the degeneration of our cells.
Reproduction in Women
- High levels of stress can cause missed periods, cramping and longer periods.
- Stress can also contribute to the delay of ovulation which affects fertility.
- Stress and the consequent fatigue can reduce sexual desire.
Management of Stress
It's not always easy to manage our stress. It can be overwhelming sometimes and it can feel like nothing can help you but, these Stress Management Techniques are tried and tested ways of helping us manage our stress levels so we can stay healthy.
- Breathing & Relaxation
- Mindfulness
- Regular Exercise
- Mutually beneficial relationships
- Counseling
There are many options out there if you feel like you aren't handling things as well as you can. You can always talk to your GP or you can call the Samaritans, if you feel like your situation is beyond medical help.
We've included some useful links below. There's always someone to help.


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Article Text
The internet has been found to be a popular source for human health information. However, there is a lack of information on pet owners' use of the internet to source pet health information and implications for the owner–veterinarian relationship. Therefore, the aim of this study was to address this gap in knowledge by focusing on UK pet owners' general use of the internet to find online pet health information and the impact of this behaviour on the owner–veterinarian relationship. An online survey targeting UK pet owners resulted in 571 respondents. Respondents reported the most frequently used source for pet health information was the internet (78.6 per cent), followed by their veterinarian (72 per cent). Veterinarians and other pet owners, however, were rated as the most trustworthy sources. The topics searched for most often online were specific medical problems (61.3 per cent) and diet/nutrition (58.5 per cent). Regarding the owner–veterinarian relationship, 42.1 per cent of participants reported discussing information they found online 'sometimes' with their veterinarian. When asked if their veterinarian recommended specific websites, nearly half (49.6 per cent) stated that their veterinarian 'never' made such recommendations, yet over 90 per cent said they would visit veterinarian-recommended websites.
- health
- pets
- human-animal interactions
- internet
Statistics from Altmetric.com
Supplementary data
Funding This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.
Competing interests None declared.
Provenance and peer review Not commissioned; externally peer reviewed.
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When toothaches and pains get more serious, nothing at all could be more reasonable than having your corroded tooth cavities extracted. However, seeing that most dental practitioners never risk patients' wellness carrying out extreme dental care operations among an unbearable pain, it is advisable to get it removed before it is actually overdue. The horrible ordeal a person with lousy cavities cannot be that piercing if the best treatment is acquired. Nevertheless, what basically reaches the spot of issue the Affordable dentist Plano is the difficult to find the rising price? This remarkable puzzler can in some way be addressed completely with a good policy plan. The point is, not everyone can pay for such for certain. Therefore, it is rather better to spend time looking for an Affordable dentist Plano than wait up to the point the cash saved in is enough for the process.
A trip to the dental professional is not really a legal requirement, but this is something everybody ought to undertake without having to be told. Oral health is important. Even though persistent flossing and cleaning can prevent teeth pains, it remains essential to schedule a dental search for each family member.
Finding a cheap provider is a difficult challenge. Dental hygiene certainly never comes very affordable. However, provided the firmer competition among dental care companies nowadays, it is never unrealistic for you to imagine you can in some way think about the surgery in the lack of your insurance cover.
Just as much as it is not possible to take chances on random professionals you bunch into, take the time to do some profound thinking and factor in the advantages and negatives of your actions. Most definitely, most inexpensive companies are the ones who are there to make a wonderful name in the industry. As inexperienced as they are, you cannot expect superiority in what they are doing.
However, those of course are not constantly the situation. You will find loads of other methods you can virtually use in working with your finances. Like the free dental care treatment centers of nonprofit organizations.
Likewise, there are churches in town that provide substantial teeth pain alleviation to cash-strapped local people with simply a little amount of cash. Taking benefit of such program makes your budget breathable.
If brave enough, you can sign up for clinical tests. Kids dentist Plano schools run regular trials that require people to be examined. By doing this, your condition is not going to be healed; you may get free dental braces for your tooth as well.
Ask your close friends for guidance as well. Going for free treatments requires accurate resolution and bravery. Your nearest friends might have better ideas. Here is the point, no matter how specified the problem is, you need to be cautious about the certification of the Childrens dentist Plano. Each tooth is not only for beauty. They really are important for your general health. Go through reviews here and there, so you can be completely guaranteed of the help of the specialist and in some way get the sleepless mind at rest.
Find more information relating to Affordable dentist Plano , and Childrens dentist Plano here.


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Key Features
Hwa Yih Gin Machine CO., LTD. is a leading Manufacturer, Supplier and Exporter of fryer, Conveyor frying machine, Conveyer Oven, Grill Plate Machine, Bakery Machine, Steam Cook Machine, Stuffing Machine with our factory in Taiwan. If you are interested in any styles of our products, please feel free to contact us.
We would like to inform you that we are a company that is dealing with making not only food machine but also baking machine. We have developed more than fifty different kinds of machine since 1988. We have many kinds of food machine which always made with the best quality. We have constantly created innovative ideas of diverse and user-friendly design to suit the exact requirement of our client ranging from small industries to manufacturing units.
Advocate "cold kitchen is a good kitchen" which has high efficiency, comfortable temperature and excellent working environment with less oil smoke. Hwa Yih Gin Machine CO., LTD. can give user the absolute assured quality; our machines use high temperature, acid, corrosion resistant SUS # 304 stainless steel material, the introduction of parts of Japan, West Germany and strict control of every detail of the production process, make the product more stable and safer to use. We implement the spirit of service, whether it is sales, counseling, after-sales service warranty, repair and maintenance will do our best.
Payment Details for Offline Orders
Conveyor Continuous fryer
The purpose of Slider Kit is to gather common slideshow-like jQuery functionalities (such as news sliders, photos galleries/sliders, carousels, tabs menus)
When tracking mouse movement, you usually need to know the actual position of the mouse pointer. The event object that is passed to the handler contains some information about the mouse coordinates.


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The anaerobic way of life comprises a great metabolic diversity and, correspondingly, diversity of energy-generating mechanisms (52). The facultative anaerobes have a respiratory system that uses preferentially oxygen, but in the absence of oxygen and presence of alternative electron acceptors, such as NO3−, NO2−, Fe(III), or others, alternative electron transport pathways are induced. The situation is completely different in strict anaerobes which can derive energy only by fermentation and/or by ion gradient-driven phosphorylation. In general, the energy yield in strict anaerobes is only a small fraction of the one gained by aerobes (43). Obligately fermenting organisms rely mostly on substrate level phosphorylation (SLP), and the energy yield ranges from 1 to 4 mol of ATP per mol of hexose fermented. However, a number of organisms have evolved additional mechanisms to increase their ATP yield. Some employ electrogenic end product efflux (51) and, in addition, some of the fermentation pathways comprise one or more membrane-bound reactions which result in the generation of a transmembrane ion gradient across the membrane (43). Typical examples for the latter are the fumarate reductase system and the Na+-motive decarboxylases (9, 36). Since the latter do not catalyze oxidation/reduction reactions, the term electron transport does not fit these mechanisms and, therefore, the more general term ion-gradient-driven phosphorylation is favored. At the end of this line are the chemolithoautotrophic anaerobes. Methanogenic archaea reduce CO2 to CH4 and do not employ SLP but have membrane-bound enzymes that couple methyltransfer reactions and electron transfer reactions to the export of Na+ and H+, respectively (7, 21). The Na+ and H+ gradients established are used to drive the synthesis of ATP by membrane-bound A1A0 ATPases (48). Although the analyses of the structure and function of A1A0 ATPases has made some progress in recent years, the ion specificity of these enzymes needs to be elucidated, and the question how both ion gradients are used to drive ATP synthesis has not been settled (47). Acetogens use a similar pathway for CO2 reduction as methanogens, the Wood-Ljungdahl pathway, but with respect to the energy conserving mechanisms they can be divided into two groups, the Na+ and the H+ organisms, which apparently differ also in some aspects of the pathway (38, 44). In recent years it became evident that CO2 is neither the only nor the preferred electron sink in acetogens but acetogens can use alternative electron acceptors (12). This review will focus on the energetics of CO2 reduction (homoacetogenesis) and reduction of other electron acceptors in acetogens.
Organisms that are able to reduce CO2 to acetate via the acetyl coenzyme A (acetyl-CoA) or Wood-Ljungdahl pathway (Fig. 1) are termed acetogens. This metabolic capability differentiates acetogens from organisms that synthesize acetate by other metabolic pathways. Acetogens are strictly anaerobic bacteria that can grow by the conversion of C1 compounds such as H2-CO2, CO, or formate to acetate (8). Phylogenetically, acetogens are rather diverse, and 19 bacterial genera have been described to date. Acetogens can inhabit very diverse ecosystems ranging from different soils to termite hindgut, and also extremophiles with respect to temperature, pH, and salinity have been found. It is estimated that acetogenesis yields billions of tons of acetate globally each year and, therefore, acetogens play an important role in the global carbon cycle (15).
Acetogens can grow on a variety of different substrates such as, for example, hexoses, C2 and C1 compounds. Hexoses are converted exclusively to acetate: $$mathtex$$\[1\ C_{6}H_{12}O_{6}\ {\rightarrow}\ 3\ CH_{3}COOH\]$$mathtex$$(1) and, therefore, this fermentation is also referred to as homoacetogenesis. The pathway of hexose consumption starts with their oxidation via the Embden-Meyerhof-Parnas pathway to pyruvate, which is then oxidized by pyruvate:ferredoxin oxidoreductase to acetyl-CoA, reduced ferredoxin, and CO2. The acetyl-CoA is then converted to acetate via acetyl phosphate. The oxidative branch of the pathway (equation 2) is coupled to the synthesis of 4 mol of ATP by SLP: $$mathtex$$\[C_{6}H_{12}O_{6}{+}\ 4\ ADP{+}\ 4\ P_{i}\ {\rightarrow}\ 2\ CH_{3}COOH{+}\ 2\ CO_{2}{+}\ 4\ ATP{+}\ 8\ [H]\]$$mathtex$$(2) Second, the reducing equivalents gained during glycolysis and pyruvate:ferredoxin-oxidoreductase are reoxidized by reducing the two mol of CO2 to another mol of acetate via the Wood-Ljungdahl pathway: $$mathtex$$\[2\ CO_{2}{+}\ 8\ [H]{+}\ n\mathrm{ADP}{+}\ nP_{i}\ {\rightarrow}\ CH_{3}COOH{+}\ n\mathrm{ATP}\]$$mathtex$$(3) During growth on sugars or other organic substrates, CO2 can be formally regarded as an electron sink and, per se, there is no need that the Wood-Ljungdahl pathway is coupled to energy conservation. Energy is gained during glycolysis, and the redox balance is maintained by operation of the Wood-Ljungdahl pathway. However, it is important to note that the Wood-Ljungdahl pathway also enables growth on H2-CO2 according to: $$mathtex$$\[2\ CO_{2}{+}\ 4\ H_{2}{+}\ n\mathrm{ADP}{+}\ nP_{i}\ {\rightarrow}\ CH_{3}COOH{+}2\ H_{2}O\ {+}\ n\mathrm{ATP}\]$$mathtex$$(4) and, therefore, it must be coupled to net ATP synthesis. The overall free energy change of the reaction (ΔGo′ = −95 kJ/mol) could allow for the synthesis of 1 to 2 mol of ATP. Indeed, it is now well established that CO2 reduction via the Wood-Ljungdahl pathway according to equations 3 and 4 is coupled to ion gradient-driven phosphorylation.
The Wood-Ljungdahl pathway is widespread in anaerobic bacteria and archaea and is used for both anabolism and catabolism (19, 39, 55, 77). First, CO2 is reduced to formate by action of formate dehydrogenase, and then formate is activated and bound to tetrahydrofolate (H4F), yielding formyl-H4F (Fig. 1). Water is split off, and the resulting methenyl group is reduced via methylene-H4F to methyl-H4F. The methyl group is then transferred to a protein containing a corrinoid cofactor and iron sulfur clusters, the corrinoid iron-sulfur protein (CFeSP). From there, the methyl group is transferred to the bifunctional CO dehydrogenase/acetyl-CoA synthase (CODH/ACS) that plays a central role in the pathway (11, 56). The methyl group is condensed on CODH/ACS with carbon monoxide, derived from another mol of CO2 oxidized by the CODH activity, to acetyl-CoA. Acetate is produced from acetyl-CoA by action of phosphotransacetylase and acetate kinase. The reducing equivalents required are gained by oxidation of molecular hydrogen (during autotrophic growth) or NADH and reduced ferredoxin (during heterotrophic growth).
The energetics of this pathway is rather interesting. One mole of ATP is produced by SLP in the acetate kinase reaction, but one mole of ATP is consumed in the formyl-H4F synthetase reaction (see Fig. 1). Therefore, the net ATP gain by SLP is zero, and ion gradient-driven phosphorylation must occur as well (for the organisms can grow chemolithoautotrophically). In recent years, experimental evidence was presented that ion gradient-driven phosphorylation indeed occurs in acetogens, but the sites of energy conservation and the mechanisms employed are different. From a bioenergetic point of view, acetogens can be divided into two groups: the Na+-dependent acetogens with Acetobacterium woodii (46) and the H+-dependent acetogens with Moorella thermoacetica (formerly Clostridium thermoaceticum) as model organisms (38). The latter group contains cytochromes and a membrane-bound, H+ motive electron transport chain. The Na+-dependent acetogens lack cytochromes but have membrane-bound corrinoids and couple the Wood-Ljungdahl pathway to primary and electrogenic translocation of Na+. The ion gradient established is taken advantage of by H+ and Na+ translocating ATP synthases, respectively.
In the pioneering work of the group of L. G. Ljungdahl, several membrane-integral electron carriers were identified in Moorella thermoacetica and the closely related Moorella thermoautotrophica (formerly Clostridium thermoautotrophicum). Menaquinone MK-7 (2-methyl-3-heptaprenyl-1.4-naphthoquinone; Eo′ = −74 mV) and two b-type cytochromes (cytochrome b559, Eo′ = −215 mV; cytochrome b554, Eo′ = −57 mV) were already identified in 1975, and later on it was shown that a flavoprotein copurified with cytochrome b559 (3, 22). These components are suggested to be involved in electron transport processes (6, 38). The nature of the electron donor and acceptor systems is less clear. It should be mentioned that all of the enyzmes of the Wood-Ljungdahl pathway were isolated so far from the cytosolic compartment after French press disrupture of cells and, therefore, their involvement in energy conservation seems questionable. However, this harsh procedure might have led to a desintegration of membrane-bound enzymes and liberation of the enzymes or catalytically active subcomplexes thereof into the cytosolic compartment. Therefore, it is a necessity to prepare inside-out vesicles under mild conditions that prevent or reduce the loss of membrane-bound enzymes. Using such vesicle preparations, it was indeed shown that certain enzymes may attach to the cytoplasmic membrane: the potential electron donors hydrogenase, CODH, and NADH dehydrogenase, as well as the potential electron acceptor methylene-H4F reductase (30).
Thauer et al. (73) suggested in 1977 that the methylene-H4F reductase may be the last limb of an electron transport chain that could lead from an electron donor such as hydrogenase, CODH, and NADH dehydrogenase via the membrane-bound electron carriers to methylene-H4F. This electron transport could result in the generation of a transmembrane proton potential that then drives ATP synthesis (Fig. 2A). Although this is a reasonable scenario, experimental evidence for either membrane-bound electron transport to methylene-H4F or the generation of a transmembrane electrochemical potential (ΔμH+) during this reaction is still missing. However, a ΔμH+ was established in membrane vesicles during oxidation of CO (by CODH) coupled to the reduction of ferrycyanide (28). The ΔμH+ was used to drive the uptake of amino acids (29). This clearly proved CO-dependent formation of ΔμH+ but did not reveal the components and mechanisms involved.
The ΔμH+ established is taken advantage of by a H+ F1F0 ATPase to synthesize ATP. The enzyme and subcomplexes thereof have been isolated from membranes of Moorella thermoacetica and Moorella thermoautotrophica (6, 32). The enzyme contained, like the H+ F1F0 ATPase from Escherichia coli, subunits α, β, γ, δ, ε, and c, but was devoid of the essential subunits a and b. Therefore, it was speculated that the architecture of the clostridial F1F0 ATPase is simpler than that from other bacteria (4). However, the genes encoding the F1F0 ATPase are organized in an operon that also contains the genes coding for subunits a and b (5). Therefore, it can be assumed that the enzyme from Moorella thermoacetica and Moorella thermoautotrophica, like that from other bacteria, has an α3β3γδεab2c9-14 architecture. The discrepancy between the biochemical and molecular data are most likely due to the loss of subunits during purification of the ATPase. Indeed, loss of subunits during purification of F1F0 ATPases is encountered very often and also observed in A. woodii in which the same subunits were missing in the purified enzyme but later on shown to be present in the in vivo complex (see below).
With the discovery that methanogenic archaea that also use in part the Wood-Ljungdahl pathway are strictly dependend on Na+ for CO2 reduction (53, 54) and that they couple the methyltetrahydromethanopterin:coenzyme M methyltransferase to vectorial Na+ transport across the cytoplasmic membrane (37, 45, 49), it was obvious to look for Na+ dependence of acetogenesis. These studies revealed a Na+ dependence for growth and acetate formation in A. woodii (26), Thermoanaerobacter kivui (formerly Acetogenium kivui) (78), and Ruminococcus productus (formerly Peptostreptococcus productus) (20). A. woodii was used as a model system to unravel the molecular basis of the Na+ dependence.
(i) Generation of a sodium motive force in A. woodii.From studies with cell suspensions of A. woodii, it was apparent that the Wood-Ljungdahl pathway is Na+ dependent and accompanied by the generation of a sodium motive force across the cytoplasmic membrane by a primary, electrogenic pump involved in acetate formation from CO2. Further studies with substrates that are fed into the Wood-Ljungdahl pathway at different levels identified the reaction sequence leading from methylene-H4F to a methylated intermediate as the Na+-dependent reaction sequence of the pathway (26). This reaction sequence contains the methylene-H4F reductase, and one (or two) methyltransferase reactions as presented below: $$mathtex$$\[methylene-H_{4}F{+}\ 2\ [H]\ {\rightarrow}\ methyl-H_{4}F\]$$mathtex$$(5)$$mathtex$$\[methyl-H_{4}F{+}\ CFeSP\ {\rightarrow}\ methyl-CFeSP{+}\ H_{4}F\]$$mathtex$$(6)$$mathtex$$\[methyl-CFeSP{+}\ CODH/ACS\ {\rightarrow}\ methyl-CODH/ACS{+}\ CFeSP\]$$mathtex$$(7) Equation 5 is catalyzed by the methylene-H4F reductase. Because the enzyme was found to be localized to 100% in the cytoplasm (R. Heise, V. Müller, and G. Gottschalk, unpublished data), we do not consider this reaction sequence to be sodium motive. Furthermore, studies with the Na+-dependent acetogen R. productus also made an involvement of the methylene-H4F reductase in Na+ transport unlikely (76). This leaves the reactions shown in equations 6 and 7 as the most likely candidates for Na+ transport. This reaction sequence has never been studied in Na+-dependent acetogens; all we know results from studies with proton-dependent organisms in which the entire sequence seems to be catalyzed by soluble, cytoplasmic enzymes (55, 69). However, in Na+-dependent acetogens, the situation might be different. The analogy of the pathways in acetogens and methanogens, as well as the finding of membrane-bound corrinoids in Na+-dependent acetogens (2), led to the proposal that the methyl transfer from methyl-H4F to CODH/ACS is the site of Na+ extrusion in Na+-dependent acetogens (46). In analogy to the membrane-bound, Na+-pumping methyltetrahydromethanopterin:coenzyme M methyltransferase of methanogens, the corrinoid could be a cofactor of a multisubunit, membrane-bound, Na+-translocating methyltransferase (Fig. 2B). However, this still has to be verified by experimental analyses.
(ii) A. woodii synthesizes ATP by means of an Na+ F1F0 ATP synthase.Upon addition of H2-CO2, A. woodii produced acetate and Na+ was extruded from the cells giving rise to a chemical Na+ potential (ΔpNa+) of −90 mV (26). Experiments with cell suspensions revealed that the Na+ gradient drives phosphorylation of ADP. ATP synthesis was not inhibited by protonophores but by sodium ionophores, indicating a direct coupling of ATP synthesis to the Wood-Ljungdahl pathway by ΔμNa+ (24). Studies with cell suspensions of A. woodii clearly demonstrated the presence of an Na+-translocating ATPase/ATP synthase (25, 27).
The ATPase was purified to apparent homogeneity after solubilization of membranes with Triton X-100 by (poly)ethyleneglycol precipitation and gel filtration. This preparation contained (only) six subunits (α, β, γ, δ, ε, and c2/3) as the clostridial enzyme (62). However, later studies using separation of membrane protein complexes by blue native-polyacrylamide gel electrophoresis and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension revealed the presence of three additional subunits (b, a, and c1) (1). Biochemical, immunological, and molecular studies identified the ATPase of A. woodii as a member of the family of Na+ F1F0 ATP synthases (17, 58, 61, 62). In bacterial F1F0 ATPases, three copies of each subunit α and β alternate around a central stalk built by subunit γ. Subunit ε is part of the central stalk and connects it with the membrane domain via subunit c, the proteolipid. A ring of subunit c (9 to 14 copies), subunit a (1 copy), and subunit b (2 copies) are localized in the membrane and build the motor of the rotary device (47). Ion flow through the membrane along the a-c interface is coupled to a rotation of the ring of proteolipids (66), and this rotation drives rotation of the central stalk (subunit γ) (79). Rotation of subunit γ within the a3b3 headpiece results in the liberation of ATP from the β subunits. Such a mechanism requires a stator, which is most likely build by subunits b and δ (Fig. 3). It is noteworthy that the same architecture and mechanisms apply to Na+ and H+ F1F0 ATPases. The ion specificity is altered by only a few amino acid changes in the motor component (see below).
(iii) Na+ F1F0 ATP synthase of A. woodii: the first enzyme with a heterooligomeric motor comprising "bacterial-like" and "eukaryal-like" proteolipids.The atp operon of A. woodii that encodes the Na+ F1F0 ATP synthase contains homologues of the nine genes present in the E. coli atp operon (17, 58) (Fig. 3). The order of the genes is atpIBE1E2E3FHAGDC. Northern blot analyses revealed that the genes form one polycistronic message. In contrast to any other known F1F0 ATPase operon, the atp operon from A. woodii contains three tandemly organized genes (atpE1, atpE2, and atpE3) encoding subunit c, the proteolipid. AtpE2 (subunit c2), and AtpE3 (subunit c3) are 100% identical at the amino acid level, and only 18 substitutions occurred on the DNA level (58). This is strong evidence for a duplication of an ancestral gene. The deduced molecular mass of the polypeptides is 8.18 kDa. As their bacterial homologues, they are supposed to be organized in the membrane like a hairpin with two transmembrane helices connected by a polar loop. Most interestingly, atpE1 with 546 bp is more than double the size of atpE2/3. The first and second halves are 66% identical on the DNA level, indicating a duplication of a precursor and subsequent fusion of the two gene copies. The deduced molecular mass of AtpE1 (subunit c1) is 18.37 kDa, with four predicted transmembrane helices arranged in two hairpins. However, the membrane-buried ion-binding residue (Glu62 in AtpE2/3; Glu79 in hairpin one of AtpE1), which is also conserved and involved in H+ transport in H+ F1F0 ATPases, is substituted by a glutamine residue in hairpin two (Fig. 4). It is important to note that homologues of AtpE1 were previously found only in eukaryal V1V0 ATPases (40, 50). These enzymes are not able to synthesize ATP in vivo, and their inability to synthesize ATP results from the fact that the ion-translocating group is missing in one of the two hairpins of subunit c. Therefore, it was important to demonstrate the presence of a eukaryal-like proteolipid in an ATP-synthesizing ATPase. Biochemical studies verified that the proteolipid oligomer of the F1F0 ATPase from A. woodii indeed comprises a mixture of "bacterial-like" 8- and "eukaryal-like" 16-kDa polypeptides, the first found in nature (1). The stoichiometry of the different polypeptides in the c-oligomer has not yet been determined, but it may vary with the substrate and may be used to change the enzymatic reaction from preferentially hydrolysis to synthesis of ATP and vice versa.
(iv) Sodium ion binding site in FO.The Na+ binding site resides in the motor component of the ATPase (34). The Na+ binding site in subunit c of F1F0 ATPases was elucidated by various techniques and groups. Glu62, Thr63, and Gln29 (numbering of A. woodii subunit c2/3) were identified as part of the binding site by site-directed mutagenesis in E. coli and Propionigenium modestum (35, 80). Furthermore, Pro25 (A. woodii numbering) of subunit c is only conserved in the two Na+ F1F0 ATPases (60). This might indicate the involvement of Pro25 in Na+ binding, but this has to be verified by site-directed mutagenesis. The sodium ion binding site is depicted in Fig. 4.
Apart from subunit c, a second subunit, subunit a, is required for ion transport; both are envisaged to make the ion channel of the ATPase (10, 16). Only one residue of subunit a from the H+ F1F0 ATPase, Arg210, was indicated by mutant studies to be directly involved in H+ transport (75). The residues involved in Na+ binding of subunit a from Na+ F1F0 ATPases are unknown, but a comparison of the sequences of subunit a from the Na+ F1F0 ATPase from P. modestum and A. woodii to those from H+ F1F0 ATPases revealed determinants likely to be important for Na+ binding (59).
(v) Why multiplication and duplication of the proteolipid encoding gene?One of the most striking and unique features of the atp operon of A. woodii is the presence of multiple copies of proteolipid encoding genes. Multiplication of proteolipid encoding genes has been found before only in V1V0 ATPases from eukarya and A1A0 ATPases from archaea (47, 48). What could be the selective pressure for multiplication of proteolipid-encoding genes? One has to keep in mind that the subunits of the ATPase are present in different stoichiometries (a1b2c9-14δα3γβ3ε), and the proteolipid has by far the highest copy number (9 to 14 copies of the bacterial 8-kDa proteolipid, depending on the species) in the complex. Most of our knowledge concerning the regulation of the synthesis of the proteolipid derived from the paradigm E. coli. There, the proteolipid encoding gene is part of a polycistronic message, and enhanced synthesis of the proteolipid is achieved by enhancement of translation (42). In addition, but to a lesser extent, regulation by differential mRNA stability contributes to differential gene expression (41). Apparently, multiplication of the atpE gene and embedding the copies into the operon is another way to increase the concentration of subunit c. This strategy is apparently realized by A. woodii, but this does not exclude additional mechanisms.
Another interesting finding is that the proteolipid of the A1A0 ATPase of the hyperthermophilic archaeon Methanopyrus kandleri is deduced from the genome sequence to have a proteolipid with 13 times the size of bacterial 8-kDa proteolipids that is encoded by only a single gene (70). The rotor is apparently monomeric and built by only one copy of a proteolipid with 13 hairpin domains. Methanopyrus kandleri grows optimally at 98°C, with a maximum at 110°C. Because the rotor is located in the cytoplasmic membrane, it "feels" the heat more intensely than heat-protected enzymes from the cytoplasm; this extremely high temperature might favor a monomeric rotor. However, the existence of a monomeric rotor is only deduced from the genomic sequence but not yet proven by biochemical data. Methanocaldococcus jannaschii and Methanothermobacter thermoautotrophicus grow optimally at 85 and 65°C, and they have proteolipid monomers with three- and two-hairpin domains (63, 64), whereas Methanosarcina mazei and other mesophiles have 8-kDa proteolipids with only one hairpin (65). Therefore, larger proteolipids could be of evolutionary origin favored by high temperature environments.
The duplication of the proteolipid encoding gene per se has no consequence for the function of the ATPase since it was shown before that genetically engineered duplicated proteolipids from E. coli are functional in H+ transport and ATP synthesis (33). The striking feature, however, of subunit c1 of A. woodii is not its size but rather the fact that the ion-translocating residue is not conserved in helix two. A loss of one ion-translocating residue is also encountered in the eukaryal "16-kDa proteolipids" from V1V0 ATPases, and this loss has the dramatic consequence that the V1V0 ATPases are not able to synthesize ATP in vivo (47). The ability to synthesize ATP is directly dependent on the number of ions translocated per ATP synthesized. According to the equation ΔGp = −nFΔp, a phosphorylation potential (ΔGp) of ∼50 to 70 kJ/mol is sustained by the use of n = 3 to 4 ions/ATP at a physiological electrochemical ion potential of −180 mV (Δp). However, if the number of ions is lower, then ATP can no longer be synthesized. Although it was demonstrated that the number of monomers in the proteolipid ring may vary from 9 to 14 (67, 71, 72), for the following calculation it is assumed that, as in the case of the bacterial and archaeal "8-kDa proteolipids" with two transmembrane helices, 12 monomers and 12 ion-translocating carboxyl groups are present per oligomer. Once we take into account three ATP-synthesizing or hydrolyzing centers, this gives a stoichiometry of four ions/ATP. In contrast, six copies of the "16-kDa proteolipid" with four transmembrane helices constitute the proteolipid oligomer of V1V0 ATPases. Since the ion-translocating group is lost in the first pair of transmembrane helices, the stoichiometry is only two ions/ATP, which is too low to allow ATP synthesis. On the other hand, if the number of ions is low, the same ΔGp can account for a much higher Δp, making the enzyme a better ion pump, a function required by the physiology of the eukaryotic cell. In general, the lower the number of carboxylates per ring, the worse the coupling efficiency. Taking this into account, it is now reasonable to assume that a cell could, depending on the cellular needs, alter the function of the ATPase between ATP synthesis and ATP hydrolysis by varying the number of ion translocating residues. This is a very attractive idea for A. woodii, which can grow by fermentation of hexoses or by anaerobic respiration or by using pathways which neither involve SLP nor respiration but most likely methyl transfer-driven Na+ extrusion during the operation of the Wood-Ljungdahl pathway. During fermentation, the F1F0 ATPase has to work as an ion pump to generate the membrane potential, whereas during autotrophic growth on H2-CO2 it has to work as a synthase. The switch from pump to synthase could be performed by changing the ratio of c1/c2/3 (Fig. 5). To test this hypothesis is a challenging task for future experiments.
As outlined above, the fermentation of hexoses (but also other substrates) yields reducing equivalents that are used to reduce 2 mol of CO2 by the action of the Wood-Ljungdahl pathway, thereby allowing the synthesis of 3 mol of acetate per mol of hexose fermented. In addition to CO2, alternative electron acceptors, such as aromatic acrylate groups, fumarate, dimethyl sulfoxide, nitrate, and nitrite can be used by some acetogens. Under these conditions, the Wood-Ljungdahl pathway may be turned off completely and only 2 mol of acetate are formed. Furthermore, acetate synthesis may be blocked completely (14). A typical substrate combination that does not yield acetate is the oxidation of a methyl group coupled to the reduction of a phenylacrylate (13). The use of alternative electron acceptors was, in some instances, shown to be coupled to energy conservation. A. woodii, for example, is known to reduce the carbon-carbon double bond of phenylacrylate ethers such as caffeate as shown in Fig. 6.
The electrons can be derived from various donors such as fructose, methanol, or hydrogen. Cell yield measurements with cells grown on fructose or methyl group-containing substrates showed not only that is caffeate used as an electron sink but also that caffeate reduction is coupled to energy conservation (74). Very clear evidence for ATP synthesis coupled with caffeate reduction was obtained with resting cells of A. woodii in which hydrogen-dependent caffeate reduction was accompanied by the synthesis of ATP (23). Recently, it was shown that ATP synthesis occurred by a chemiosmotic mechanism (31). Most interestingly, like CO2 reduction, both hydrogen-dependent caffeate reduction and ATP synthesis coupled to caffeate reduction were strictly Na+ dependent, and the latter was dependent on a transmembrane Na+ gradient. These studies were fully compatible with the following sequence of events: caffeate reduction → generation of a transmembrane Na+ gradient → generation of ATP by the Na+ F1F0 ATP synthase (31).
It is likely that the electrons are channeled from hydrogen to caffeate via a membrane-bound electron transport chain. Oxidation of hydrogen is catalyzed by a hydrogenase and, in earlier studies, a hydrogenase was purified from A. woodii (57). Because more than 99% of the activity was found in the cytoplasm, the enzyme was described as a soluble, cytoplasmic enzyme. This suggests that an additional electron carrier such as NAD+ or ferredoxin transports the electrons to the membrane and would require a membrane-bound NADH dehydrogenase or reduced ferredoxin dehydrogenase. On the other hand, it cannot be excluded that a membrane-bound hydrogenase might be present as well. After oxidation of the electron donors, the electrons are transferred to the acceptor, caffeate. Cytochromes or quinones were not detected in caffeate-grown cultures (74) and, therefore, the components involved in the electron transport from hydrogen to caffeate are still obscure.
The utilization of caffeate is induced by caffeate, and in the presence of both caffeate and CO2, acetate is no longer produced from CO2. The site of repression of the Wood-Ljungdahl pathway in A. woodii is unknown. On the other hand, it was demonstrated the H+ organism Moorella thermoacetica uses preferentially nitrate as an electron acceptor (68). In the presence of nitrate it does not contain cytochrome b and, therefore, acetate can no longer be produced by the Wood-Ljungdahl pathway (18).
Although the elucidation of the mechanisms of energy conservation in acetogens is still in its infancy, it turned out that A. woodii is one of the rare cases in which the entire energetics (substrate accumulation, flagellar rotation, and ATP synthesis) is based on an Na+ current across the cytoplasmic membrane. However, the ΔμNa+-generating enzyme is still unknown, and future experiments should address the nature of this Na+ pump. The Na+ F1F0 ATPase of A. woodii is of special interest since it represents the first ATPase that combines features of bacterial and eukaryal enzymes. Whether this might enable the organism to regulate its cellular energy metabolism depending on the growth conditions remains to be seen. The electron transport and the ΔμH+-generating mechanisms in H+-dependent acetogens still needs to be solved. Furthermore, elucidation of the bioenergetics, enzymology, and regulatory processes involved in the utilization of alternative electron acceptors presents challenging tasks for future studies. Genome analyses will certainly pave the way toward a better understanding of the energetics, biochemistry, and physiology of acetogens.
I am indebted to my coworkers for their excellent work.
I thank the Deutsche Forschungsgemeinschaft for continuous and generous support.
- Copyright © 2003 American Society for Microbiology
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Under construction 237.000€ - Apartament
For sale apartments in Cabo Roig
Apartments in Cabo Roig located in the Costa-Blanca coast, south-eastern part of Spain. Although this time providing new trends and the luxury that this are requires.
Aguamarina is one of the loveliest areas on the Costa Blanca, surrounded by all kinds of services and of coursethe best beaches, which we have only a few meters away.
These properties are designed exclusively with first class quality materials and studied in detail for those exquisite customers.
The complex also has a stunning and inviting swimming pool surrounded by spacious and lovely communal gardens, as well as an indoor heated swimming pool and sauna


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Modeling the Complexities of the Carbon Cycle Utilizing Excel
This set of activities is about carbon sources, sinks, and fluxes among them - both with and without anthropogenic components.
Go To:
Andrea Bixler
Lindsay Dubbs
Dave Finster
Harold Geller
Jeanne Troy
CLEAN Community Collection
Notes From Our Reviewers
The CLEAN collection is hand-picked and rigorously reviewed for scientific accuracy and classroom effectiveness. Read what our review team had to say about this resource below or learn more about how CLEAN reviews teaching materials
Teaching Tips
Literacy and Resources
Relation Link:


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Every day there is a little something we can do to brighten someone's day. Small things can change other people's lives in a way one cannot imagine. Equally, small things can make our day as well. Remember – what goes around comes around.
1. Smile. Whether you're waking up, heading for the office or running household errands, just smile. Simply flash a smile to someone dear to you or to a complete stranger. It can make their day. It can make your day, too.
2. Surprise. Surprise someone with a flower, a freshly brewed coffee or an invitation to dinner at your house. Be the person who spices up other people's lives.
3. Listen. Put everything else aside and lend an ear to someone who needs it. Sometimes we only need to share our thoughts with another to feel better.
4. Share. Share dessert with your new neighbours. Share good ideas with your colleagues. Share positive energy with a person you've just met for the first time. Sharing is caring.
Morning bird or not, there is no better feeling than to start off the day with a healthy breakfast and a cup of delicious coffee or a tea. Attractive morning duo of the Gorenje Infinity collection, which includes the kettle and toaster, will brighten your kitchen – and your days!


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The hiring of a law firm is an important decision that should not be based solely on advertisements. Before you decide, ask us to send you free written information about our qualifications and experience. Personal Injury Lawyers serving Daytona, Melbourne, Orlando, Florida and Nationwide.This website is not intended for viewing or usage by European Union citizens.
Posted: September 1, 2011
New regulations proposed by the Federal Motor Carrier Safety Administration (FMCSA) are designed to prevent a common cause of fatal trucking accidents, driver fatigue. The FMCSA proposes to reduce the number of hours a trucker can drive during every fourteen (14) hour shift from eleven (11) to ten (10) hours of driving. The new regulations would also require truckers to take a one hour break during long work shifts that include loading and unloading their cargo and to take more time off between seven (7) day stretches of road work.
Last year, the agency approved a rule that would require truckers who have a history of repeat violations of the driving hours limits to install electronic devices that would replace easily doctored paper log books. Another rule being proposed would require all truckers to have electronic recorders, whether or not they had a history of driving excessive hours or falsifying their log books.
If you have been involved in an accident with an interstate trucker, you need a law firm that is experience and knowledgeable about the applicable trucking regulations, trucking standards, and the trucking industry. CGWC has attorneys who are experienced in handling interstate trucking accident cases.


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Birthday: 10th April
Location: Nagoya, Japan
Yuria is a 20 yr old girl who had been trained in classical ballet so is elegant and has a calm demeanor. She now lives in Nagoya and works. She loves to go to clubs, traveling, loves spas and her favorite drink is sake.
She's into big muscular men like Takeshi Kaneshiro who can pick her up and make her their little girl. Her dream is to be a jewelry designer.
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Don't forget your membership includes these sites too!


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After Pikachu completely destroyed them in the first couple episodes, Team Rocket's view of Pikachu being a worthless Pokemon changed to one of 'this Pikachu is special'. Because of this Team Rocket just follows Ash around in order to try catch Pikachu and give it to their boss Giovanni.
Most of the time before they formulate some plan they start thinking of money, approval or PROMOTIONS :D
After their repeated failures they basically settled into getting any Pokemon - not neccesarily just strong ones. Well their end-goals are to get the strong ones, but they're fine with getting weak ones on the way. Also they kind of consider most of Ash's Pokemon at quite a high level :D
Well in the first few episodes, they think Pikachu is worthless and weak. But they find out that Ash's Pikachu is special and strong. They then make it their mission to capture Pikachu and impress the boss. But along the way, they'll pretty much take any Pokemon that they can get their hands on that they don't think is a weakling.
Source: I recently watched season 1. Hope I helped! :)


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Tina M. Thomas
Tina M. Thomas is an associate in the firm's Washington, DC office, where she practices in the White Collar Defense and International Arbitration practice groups. In her White Collar Defense practice, Ms. Thomas handles major internal investigations of sexual misconduct at schools and other institutions; the representation of individual clients charged with financial fraud; and the advisement of leading technology companies on privacy and data protection issues. In her International Arbitration practice, Ms. Thomas represents foreign nations and companies in international disputes, especially those relating to the Russia-Ukraine conflict.
Ms. Thomas also maintains an active pro bono practice, specializing in criminal defense and voting rights issues. Among her matters, she represents indigent criminal defendants in the Washington, DC metro area. She also advises the National Redistricting Foundation on litigation to challenge partisan and racial gerrymanders.


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Atagoyama Nashiji Kiita lv 4 (a706)
£684,56 (Inc. VAT)
£547,65 (Ex. VAT)
VERY Beautiful and Huge Yellow Atagoyama Kiita !
With Yellow colors. Very Rare to find such big stone with so nice and even colors!!
It will last for many many generations.
Super clean without any inclusions or lines that can be felt at all!
Definitely collector peace !!
Can be used for Knives and Fine tools but also razors as it is super fine.
Very smooth feel while sharpening !!
Recommend Nagura
Size: 220mm 85mm 70mm


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In early 2014, when Ken Levine decided to let go of the BioShock franchise with Irrational Games it was a sad day for all. Fortunately, Take-Two CEO, Strauss Zelnick, remains positive about the game's future.
We do believe [BioShock] is beloved. We think it's important [and] certainly something that we're focused on.
Though this promising news is now over a year old, Zelnick currently reaffirmed his stance over the weekend at MKM Partners Investor Day Conference. He's directly committed the franchise's future to 2k games.
BioShock is unquestionably a permanent franchise in our company and one that we do believe in. The franchise is in the hands of 2K.
Though the future of such a popular series still remains a mystery, we are hopeful to see the next installment of BioShock in the future.


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Coming soon!
Melt Into Bliss October Getaway
October is here and our much anticipated Melt Into Bliss Getaway has arrived! This extra special getaway has been crafted just for you. We know that a full spa day is much overdue, so be sure to take advantage of this amazing getaway package while it is here!


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It would be really great to be able to record to non-continuous timecodes. IE if I have 3 minutes of audio at 01:00:00:00, and then 5 minutes of audio at 01:15:00:00, I shouldn't have to record the 12 minutes of silence in between. I know that I can punch in/out to timecodes that have already been recorded, but I'd love a solution that allows the user to jump to new timecodes
One logical development avenue would be to allow the user to define the FFOA and LFOA when creating the master file - DAPS could then create a timeline with silence from beginning to end so that the user can then freely punch in and out in between
Hi James,
Interesting idea. But you can always record separate masters at the different timecodes and then stitch them together adding the silence that is needed in the Dolby Atmos Conversion Tool. For now this requires the use of .xml file but a UI version is in the works that will make this intuitive.
Thanks for your reply Adam - I haven't used the Conversion Tool before - I've just downloaded it to take a look.
In truth my usage scenario is probably not 'typical' in terms of how the software was designed - I'm doing sound design for features and do so in Atmos so that material is presented as best as possible to the mix stage, requiring as little effort as possible on the mixer's behalf. Sometimes the mixer is me, sometimes not - but the idea is to do as much spacial preparation/panning as possible in the edit room so that the mixer can focus on the 'big picture' stuff, rather than the small details.
To that end, I do not use DAPS as a final delivery tool, but primarily for monitoring. Of course, I regularly need to export material to pass on to other departments (sound supervisor, editorial, temp mixes, other sound editors, etc), and for this reason DAPS and its re-rendering is fantastic - each department invariably wants material in different formats/widths, and DAPS makes all this really easy to manage.
The reason for the initial feature request is because I'm only working on specific points in the film, often with large spaces in between - and so really what I'm asking for is a time effective way to record out material to pass on to others. Although I haven't tried it yet, my hesitation with the Conversion tool is that it will still require extra time to get to the end result
Hopefully you find this helpful ;)


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Ron and I were chatting last night about my recent blog about the importance of supporting local young entrepreneurs. Suddenly, Ron jumped up from the couch (quite painful after a hernia operation), disturbed Bella's reading (!) and said he had an idea (he usually has).
We know that there are quite a few young (and not so young) people on the island who are in the early stages of starting their own food business. It maybe that you are just thinking about starting your own food business and haven't got around to actually baking or cooking. You are, perhaps, cooking in your domestic kitchen…. or wondering where you can prepare your artisan goodies.
What Ron and I can offer you is the use of our artisan kitchen (when we aren't using it) to help you try out your recipes or prepare for your events. Of course, we use our kitchen quite a lot (!!!!) but there are times when we do not. In high season, I guess we would have one day per week to offer and weekday evenings. Off season, there would be more time available.
We can't do this for free – we will need to charge a fee ! But ….. you never know…. we might even be able to sell your products in our shop !!!!
These are initial thoughts based on our conversation last night in front of the TV. If you are interested, get in touch with our P.A. (seen here in the photo!) on 01248 410320 or email us at email@example.com
Margaret x
1 Comment
We have just moved to Angelsey, five days ago to be precise. Having owned a static caravan here for more than fifteen years. I have really enjoyed reading your blog and will definitely be visiting you in the New Year x


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The purpose of this organization is to provide a forum for community issues and concerns. The association brings together community members for social and community awareness. The SCA is the sponsoring organization for Cub Scout Pack 152, Sewanee Classifieds, and the Sewanee Community Chest. Any adult who resides in the area and shares concerns of the community is eligible for active membership.
Meetings are scheduled September 4, October 2, November 13, February 5, March 5 and April 16 at the EQB House. Social time with wine begins at 6 p.m., and dinner begins at 6:30 p.m. The business meeting begins promptly at 7 p.m, followed by a brief program. The program portion of the evening is free and open to the public.


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With your expert Blue Badge guide explore the British capital. See the iconic landmarks on this 3-hour walking tour. You have your own private guide making sure that you maximize your time without feeling rushed. Take a stroll around Covent Garden, Leicester Square and Piccadilly Circus, see Trafalgar Square, Westminster Abbey and of course, Big Ben! And never miss the Buckingham Palace
and some royal history and juicy insider secrets! This tour could be custom tailored and extended to include the Tower Bridge, Tower of London and St. Paul's Cathedral.


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|By Vince "Fitness Financier" Pe|
This month, I had the pleasure of sitting down with Metro Toronto Police Officer – Peter Rampat to discuss what it's like to protect our city and give back to our community. I've known Peter since high school and can tell you how serious of an individual he is when it comes to protecting Toronto's fellow citizens. What I didn't know about Pete was that he is also heavily involved with an organization called ProAction Cops & Kids (www.copsandkids.ca).
ProAction Cops & Kids has been active for 20 years and their mission is to build trust between our police officers and our youth by keeping the kids involved in fitness (and other activities) and away from trouble. With a proven track record of turning around the lives of hundreds, maybe thousands, of children, ProAction wins the war on crime before it even starts with their pro active approach.
Officer Peter Rampat is on the forefront of some of the most positive changes in the community by offering free martial arts to troubled youth. With Toronto being one of the fastest growing cities in the world, the potential for our youth to be involved in crime is a troubling topic. Thankfully, officers like Pete are there to make a difference and build a Strong Community.
Q: First of all, big thanks to you for protecting and serving on a daily basis. What are some of the most difficult things about policing?
Two of the most difficult things for me as a police officer is one, being stereotyped as a mean robotic officer who is always looking to arrest someone. I'm a very outgoing and personable person and love interacting with people; sometimes the uniform gets in the way of that.
Two, maintaining a workout schedule to stay healthy and fit. I work shifts that alternate between days, afternoons and midnights so my body clock is always trying to reset.
Q: It's not often highlighted in the media, but what are some of the best things about being a police officer?
One of the best things is affecting people's lives in a positive way. The movies always seem to portray cops as always making the big bank robbery arrest or saving the day with a big gun fight and car chase, but the small things often get minimized like being able to give positive advice and referrals to help make long term changes in peoples' lives or going the extra mile trying to find out who stole money from an elderly persons bank account….there are so many more examples like these that for me make me feel good about using my skills to help others.
One of the best things about being a police officer is affecting people's lives in a positive way.
Q: How important is it to integrate fitness into your line of work?
Integrating fitness into my job is paramount for so many reasons; 1. The physical toll shift work takes on my body can be balanced with proper diet and exercise. 2. Often times during an arrest I might have to struggle with someone before I actually get them under control so good cardio and muscular endurance is a must. 3. Common in policing are foot pursuits which start off at a full sprint and slowly tapers off to a run sometimes running up stairs or climbing fences until I make contact with my suspect. When contact is made the task of actually hand cuffing the suspect is still left to be done which requires your body to be in top condition.
Q: What does your training regimen in the gym look like?
When I go to the gym I try to replicate various situations I may encounter on the street. To prepare for a foot pursuit I will do 20 minutes of interval training on the treadmill to push my muscles and lungs to different levels of exertion. When I do weights I target every muscle and do higher reps (15-20) with 60 seconds rest in between to work on my muscular endurance.
When I go to the gym I try to replicate various situations I may encounter on the street.
Q: You are also a martial arts instructor. Has that been helpful on the field of duty?
I've been training in traditional Japanese Jiu Jitsu since 1991. I became an instructor in 1997 and began to teach at the university of Toronto for 6 years. Our training is practical and intense to meet the physical demands of a violent self-defense encounter. I have been a police officer for 8 years and I can say that my Martial Arts training has given me confidence in any scenario and has provided me with the skills that have allowed me to overcome resistance during an arrest. In my line of work this means the difference between staying safe or getting hurt.
Q: You have a martial arts foundation for kids. Can you tell us about it?
The foundation that I work under is called Pro Action Cops and Kids. This foundation encourages friendships and community ties between police officers and youths in the pursuit of creating a positive relationship between youths and police.
Q: Why did you start this foundation for kids?
I chose to get involved with them because as a youth myself Martial arts clubs were expensive and I wasn't able to attend although I had a love for it. I thought that with my skills and personality I could offer free Jiu Jitsu classes for kids 13 to 17 in the hopes of tearing down some of the walls the police uniform often times creates. I am grateful that Pro Action cops and kids have funded my efforts for the past 3 years now as I have seen the results.
Pro Action Cops & Kids encourages friendships between police officers and youths.
Q: How has it been received by the troubled youth you guys are targeting?
When I first started the program it was in a high school in Scarborough, in the east end of Toronto. There were some mixed feelings by some youths about joining because it was being run by a police officer. As time went by I had a full class of students who persisted and have been climbing the ranks. My motto has always been that I will never turn anyone away BUT expect to work hard for your belt because nothing in life is free. To date I have only had one or two students drop out of my classes simply because they had joined to learn how to hurt people and not how to work hard. The Martial Arts has always been surrounded by a warrior code or Bushido which teaches many facets required to master life…some of these are respect, discipline, humility, willingness to help others, self-control etc. If a student comes to me not willing to learn these things they will not last.
Q: Do you have any particular students that stand out? Kids that you've helped save with your foundation?
I can say with honesty that all my students stand out in some way of their own. I have watched all my students develop into different more positive kids whether it is in their physical conditioning, ability to create friendships, helping others during class, being respectful to the instructors and generally being a positive person.
Martial Arts has always been surrounded by a warrior code or Bushido which teaches many facets required to master life.
Q: I love that UFC has taken all forms of combat and turned it into a disciplined sport. Do you think mainstream MMA has helped with the interest in what you're doing?
I believe that MMA has done a good job of bringing martial arts to people who maybe didn't know it existed. It has definitely created a demand in the martial arts world for MMA clubs worldwide. I'm not sure what motivates the kids to come to my club but if it is MMA it's not a bad thing. I believe that kids should start learning martial arts in a discipline that has a recognized belt system and focuses on the tenants of budo (warrior's code). When they have become proficient at this they can cross train into the world of MMA. Some of the greatest fighters in the MMA have followed this route; Anderson Silva as disciple of Brazilian Jiu Jitsu and George St. Pierre a disciple of Karate and now many other styles of martial arts. I find both of these champions to exemplify the warrior's code.
Vince "Fitness Financier" Pe
Vince Pe, founder of the Fitness Financier, has spent the last ten years in the fitness and bodybuilding industry. He has worked for some of the most prominent sports nutrition companies in North America, dealt closely with the IFBB, NPC, WNBF and WBFF organizations, and managed some of the industry's top athletes, including Mr. Olympia champions. Vince will be a regular contributor with feature interviews from some of the most interesting personalities in bodybuilding, fitness and martial arts. For more info about Vince, check out his company website at www.fitnessfinancier.com.


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To the heart of a winter wonderland with E-Aviation
Winter fun beckons not only in the Arctic Circle, but also in the Alps.
A variety of fashionable ski resorts in Austria, France and Switzerland offer the magic of the slopes, après-ski, an authentic Alpine experience and cosmopolitan flair. A unique mix that delights royals, VIPs and winter sports enthusiasts from all around the world. With the E-Aviation fleet, dreams of the slopes can easily become a reality, as you are flown straight to the most popular ski resorts.


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Over £1.5 Billion Investment Represented at Europe's First Ever Daily Deal Event
01 Mar, 2012, 16:27 GMT
LONDON, March 1, 2012 /PRNewswire/ --
DD Summit Europe brings together top industry players for the first time
Europe's first ever daily deal event, the DD Summit Europe 2012, will bring together over a dozen of Europe's most important daily deal providers on one stage in London.
The event's key speakers have between them seen venture capital investment worth over £1.5 billion in the burgeoning daily deals industry since the sector took off in Europe just two years ago.
The summit, which will be held at in London on Monday 19 March, will bring together industry leaders from across Europe to discuss the rapid evolution of the daily deal industry.
With around 70 daily deal sites active in the UK, over 1,500 deal providers across Europe and more than 200 new sites launched into the European market over the last six months, the daily deal industry is undeniably on the rise.
The three giants of the European daily deal industry will share a stage for the first time at the summit; Tobias Tschoetsch, UK & Ireland MD for market leaders Groupon, Brian Harrison, global CEO of kgbdeals and Peter Briffett, MD of Living Social UK will all address the delegates.
"The DD Summit provides a forum for daily deal professionals to share their thoughts on recent acquisitions, to advise start-ups, and to discuss the economic influence of the daily deal industry," says Stavros Prodromou, Director at DD Summit Europe.
"We have a fantastic line up of speakers from across Europe who will be covering a huge variety of topics including the future of ecommerce, customer acquisition, flash sales and hyperlocal best practices," continues Stavros.
The summit will also hear from daily deal experts Lopo Champalimaud, co-founder and CEO of Wahanda; Fabian Heilemann, co-founder of Dailydeal.de (Germany); Gerard Doyle, CEO of Discountvoucher (UK); Joaquin Engel, co-founder and CEO of Groupalia (Spain) and Esra Sertoglu, CEO of Groupanya (Turkey).
Notes to Editors
Venture capital funding in DD Summit Europe speakers:
Company Speaker Position Investment Groupon Tobias Tschoetsch MD UK & Ireland GBP881 million Living Social Peter Briffett MD UK GBP508.5 million Director of Business Foursquare Omid Ashtari Development GBP45 million Dailydeal.de Fabian Heilemann Co-founder GBP5.9 million* Huddlebuy Saurav Chopra CEO & founder GBP350,000 Goodypass Kate Garraway Founder GBP100,000 Wahanda Lopo Champalimaud Co-founder GBP7 million Qypedeals Richard Dennys CMO GBP14.5 million Groupalia Joaquin Engel CEO GBP30.4 million Citypockets Cheryl Yeoh CEO & co-founder GBP484,600 Kgbdeals Brian Harrison Global CEO Undisclosed Local Response Nihal Mehta CEO & co-founder GBP4.1 million Grupanya Esra Sertoglu CEO Undisclosed GBP1.5 billion
*Dailydeal.de - plus additional undisclosed sum
About DD Summit Europe
DD Summit Europe is the first ever daily deal event for Europe. Established by daily deals expert Stavros Prodromou, the event brings together all of the key players from across Europe, for the first time. The daily deals market has grown into a multi-billion pound industry over the last two years and the DD Summit Europe is a forum to share best practice and discuss future trends in this burgeoning industry.
Confirmed speakers include Peter Briffett, MD of Living Social UK; Brian Harrison, global CEO of kgbdeals; Tobias Tschoetsch, MD of Groupon UK; Kate Garraway, founder of Goodypass, Lopo Champalimaud, co-founder and CEO of Wahanda and Omid Ashtari, director of business development (Europe), Foursquare.
Also speaking at the event are Parmy Olson, London bureau chief at Forbes Magazine; Cheryl Yeoh, co-founder of Citypockets; Ray Weaver, assistant professor at Harvard Business School and Giuseppe Zocco, co-founder of Index Ventures.
The inaugural event will be held in London on 19 March 2012 at the Grange Hotel, St Paul's.
To find out more and register for tickets go to ddsummiteurope.co.uk
For media information and press passes please contact:
Helen Batte / Hannah Stalder / Jasmin Othman
Rooster PR
Email: firstname.lastname@example.com
Phone: +44-(0)20-7953-8776
SOURCE DD Summit Europe


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SAN FRANCISCO (CBS.MW) - This is one for the business history books: A successful company that invariably attracts swarms of competitors reaches a point of vulnerability that ends in a decision to sell itself to one of its two major partners.
Overture Services
It was only a matter of time before Overture would have to stop playing Switzerland. Indeed, earlier this month, Overture agreed to sell to Yahoo for $1.6 billion. See full story.
The question now is: What happens to Microsoft's MSN business? In an interview with CBS.MarketWatch.com, Overture's 39-year-old CEO Ted Meisel said that Overture will aggressively try to keep Microsoft but Meisel suggested that Yahoo's not betting on having that relationship.
Q: What was Microsoft's response to the Yahoo/Overture deal?
Ted: We talk to Microsoft every day. We've communicated to them that we're completely focused on serving them well. And, that we're better positioned to serve them well.
Q: How are you better positioned being integrated into Yahoo?
Ted: To the extent that we can accelerate product development efforts and provide Microsoft higher quality products as well as content-match services, that ultimately helps Microsoft. By virtue of operating as a separate unit we are afforded the certainty of having the largest traffic source - Yahoo - as a guaranteed partner and can integrate additional services into Yahoo's various vertical markets both in the U.S. and internationally. In addition, we are able to operate as an independent unit and can continue to provide our current and future distribution partners with high-quality products and services.
Q: How are you going to maintain the MSN relationship? Is it worth giving MSN a higher revenue share of the traffic it provides?
Ted: We're going to be aggressive on all fronts. It's not appropriate [to discuss revenue-share strategy].
Q: To what extent does the loss of MSN business concern you?
When we announced the deal with Yahoo, Yahoo's CFO [Susan Decker] said that Yahoo could justify this acquisition based on this network and its traffic alone. The deal makes sense without the MSN partner network. To Overture, the partner network is critical. To Yahoo, it's a highly diversified company, and I would guess that Terry Semel [Yahoo CEO] would tell you that if any one thing didn't work, it wouldn't be the end of the world. But I'll be sweating the success of the partner network.
Q: Did your company seek other offers?
Ted: The only thing I'll say is we were not for sale and did not engage in an auction process.
Q: Has Microsoft approached the company with a counter offer?
Ted: I simply can't go there. No comment.
Q: Is Yahoo a better partner than Microsoft?
Ted: Yahoo is appealing because of its focus on search and importance of search. Yahoo had that focus from a consumer perspective to build a huge audience of search. The work they've done to create content in various verticals to which we think we can add value.
Q: If you lose the MSN business, what would happen to revenue per click?
Ted: We expect revenue per click to remain at its current growth rate. We think we've established our RPC and brought it to a certain level of scale. In addition, we are constantly improving our matching technologies - that is, how searches get matched to advertisers' listings - that will increase the number of leads to advertisers.
Q: Revenue per click rose to 40 cents in the second quarter, what drove that higher and where do you see that going by the end of the year?
Ted: It was driven higher by the same things in general: advertisers realizing value from clicks, from the leads, being able to measure that value. There was more competition [from adding more advertisers]. We said 40 to 42 cents previously on a worldwide basis. The war in the first quarter skewed the mix of searches to be more about information and less about commerce. On average, revenue per rises 1 to 2 cents per quarter. We don't believe that it will grow at the same rate in the second half of this year because we're accelerating our efforts to obtain additional and multi-word search listings that have typically been searched less frequently. As a result, we'll get
Q: How does integrating with Yahoo lift that revenue per click? Or, do you feel that volume will make up for steady revenue-per-click rates?
Ted: I don't know that it does. Our belief is that we saw opportunities in additional properties - Europe and Asia. Together we saw the upside opportunity in revenue per click regardless of whether we're working together or not. We saw opportunities in verticals. One of the ways we drive revenue per click is accelerating new product development to make it easier for advertisers to understand their product, have a captive source, make it easier for them to plan on sales per click.
Q: Traffic acquisition costs, or TAC, fell slightly to 63.7 percent in the second quarter from 64 percent in the first quarter. What's the trend in TAC?
Ted: The trend has been increasing because the rate at which we and other partners have been increasing it. I would expect that continue in the foreseeable future. That 64 percent is a blended rate (includes network traffic that doesn't carry TAC). I don't expect that we would be reducing revenue share to partners. I think it's generally at a fair level. Overture will be very focused on the revenue line. And [as part of Yahoo], we could afford to share more with partners.
What a week...
Meanwhile, EBay
The stock traded at as low as $109.11, before settling at $112.24, down 3 percent. See full story.
Late Thursday, EBay reported second-quarter results that topped analysts' expectations. EBay also posted its third sequential slowdown in U.S. revenue growth. But shares were off Friday after EBay said it expects to earn $1.47 per share, excluding certain charges, on sales of $2.08 billion in 2003. Analysts had projected, on average, $1.46 a share on sales of $2.09 billion.
Priceline.com PCLN jumped 26 percent to $31.85 on Friday after the online travel agent posted second-quarter net income that topped expectations. Priceline.com also gave an upbeat outlook for third-quarter profits. Priceline.com reported second-quarter net income of $7.7 million, or 20 cents per share, on sales of $239.6 million. This compares to earnings of $6.3 million on sales of $304 million a year earlier.
Following Priceline.com's solid results, investors turned to InterActiveCorp IACI. In anticipation of upbeat results from InterActiveCorp's Expedia, investors drove InterActiveCorp, which is expected to report quarterly results in early August, saw shares rise 3 percent higher to $41.13.
Ask Jeeves ASKJ gained 2 percent to $18.52. The stock gained 27 percent on the week. The search engine, which receives revenue from Google for being a distribution partner, saw quarterly sales jump 66 percent to $25 million. The high-margin business of being a distributor of advertising listings that are accessed through online searches enabled Ask Jeeves to turn a profit in the quarter.
Receive column via e-mail. Sign up for Bambi Francisco's Net Stocks at CBS.MarketWatch.com. You can also subscribe to Bambi Francisco's Net Sense, a weekly commentary. See the latest column: The Yahoo accelerator and Ten red flags in this bull mania.


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BRUNSWICK — One of the architects working on planned renovations at Coffin Elementary School and Brunswick Junior High School on Monday presented the work his firm has done.
This is the beginning of the second phase for the school facilities plan, which has received an appropriation of $174,500 from the Town Council. Estimates from the first phase found that planned renovations could cost the town up to $21 million.
"We've reached a point where we're beginning to make decisions and observe things that we felt that the full board would be best apprised of, and also the public at large," board Member Rich Ellis said before introducing PDT Architects principal Lyndon Keck in a special meeting Monday night.
Keck ran through proposed locations for the School Department's new bus garage, three different driveway and parking configurations between Coffin and Brunswick Junior High School, and the current blueprints for proposed changes at the two schools.
Keck said changes in the two schools' driveway and parking configuration were based on feedback from the board's Facilities and Maintenance Committee four weeks ago.
One of the the three options he presented was clearly preferred by people at the meeting. It would create a one-way road between the two schools from Barrows Street, with many parallel parking spots, and become a two-way road near the schools' main entrance on Columbia Avenue.
Buses would enter from Columbia Avenue, go into a clustered parking configuration in front of Coffin and exit onto Barrows Street.
"One of the things we were trying to do overall is we've been trying to separate as much as possible the buses from the automobiles from the truck service," Keck said, "and we've tried to make it possible for pedestrians to more easily navigate the site by introducing more sidewalks on the site."
For Coffin, Keck said, significant expansions are planned, including a new wing that includes a kitchen, cafeteria, library and several classrooms.
Because of Coffin's planned expansion and the current bus garage's adjacent location to the school, architects will have to find a location to build a new garage.
Four locations were assessed, Keck said, but one seems the most viable so far: the site of the old Times Record Building. He said it would be Brunswick's least expensive option, because the town already owns the lot, and it includes existing utilities and the deparment's current bus fueling station. The former newspaper building at 6 Industry Road is now being demolished.
For Brunswick Junior High, changes include widening the administrative offices and constructing new rooms for alternative education and art classes, along with common areas for each grade and a central courtyard.
A few board members suggested changes to the plans presented by Keck, and Ellis said the board will continue to solicit ideas and concerns on the School Depatment's website and at future meetings, including one on Dec. 19.
Ellis said the school facilities plan's second phase is expected to finish in Feburary. A construction bond referendum could follow.
"If we can get the project rolling in June, that's great," Ellis said, "so to do that, we need to bring it to a vote in either June or there's been some discussion of bringing it in April, but we're going to work through this process to afford the appropriate input."
Dylan Martin can be reached at 781-3661 ext. 100 or [email protected]. Follow him on Twitter: @DylanLJMartin.
Sidebar Elements
The former Times Record building at 6 Industry Way, owned by the town of Brunswick, is being demolished. The property is under consideration for use as a new School Department bus garage.
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Liver cancer for more than 20,000 deaths in the United States each year. It begins in the liver and spreads in the body over time. Unlike other cancers, liver cancer is not necessarily painful because the body does not have nerve endings. Pain occurs when the liver has gotten bigger and stretched his blanket. Some symptoms of liver cancer include loss of appetite, massive weight loss, itchy skin, swollen abdomen, yellowing of the skin and whites of the eyes, and feel constantly sick.
The main causes of liver cancer are excessive alcohol consumption and obesity. However, factors such as age and genetics may contribute to disease. If there is good news on the diagnosis of the disease is that most patients who are diagnosed with liver cancer often survive for five years, regardless of whether they receive treatment for the disease or not. The survival of a patient depends on several factors such as cancer stage, the functionality of the liver, the patient's general health, and levels of alpha-fetoprotein in the body. If you or someone you know has been diagnosed with liver cancer, then you may want to consider including in your diet Agaricus blazei.
Agaricus blazei is a medicinal mushroom in origin in the mountains of Brazil. Aboriginal people who consume this fungus on a regular basis are said to live longer and healthier lives. They also rarely suffer from serious illnesses and degenerative diseases. These claims eventually attracted the attention of the medical world, and samples of the fungus were sent to Tokyo University in Japan for further study.
Agaricus Blazei is also known as "Mushroom of God" because it contains vitamins, amino acids, and beta-glucans. According to studies, beta-glucans can activate body cells natural killer, which serve as the first line of immune defense against foreign bodies. In addition, Agaricus blazei is supposed to lower cholesterol, prevent peptic ulcers and osteoporosis, circulatory ailments and heal.
Agaricus blazei fungi have a convex cap having a diameter of 5 to 18 cm. The cap color can vary from white to grayish or reddish brown. Agaricus Blazei is pretty sweet and has the same scent as almonds. It can be used in soups, fondues, teas, or simply as a salad dressing.
If you do not like the idea of fungi in the mouth, then you can try taking a day drops of Agaricus blazei extract. Visit your doctor first for prescriptions and directions for proper use. However, do not rely on the healing prowess of the fungus alone, because it takes more than effort and medicine liver cancer battle.


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AICPA offers valuation guide for private equity and VC firms
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The American Institute of CPAs has released a valuation and accounting guide for private equity and venture capital firms to help them determine the fair market value of the companies in their investment portfolios.
The guide, Valuation of Portfolio Company Investments of Venture Capital and Private Equity Funds and Other Investment Companies, offers nonauthoritative guidance and examples for preparers of financial statements, independent auditors and valuation specialists about the accounting for and valuation of portfolio company investments held by PE and VC firms. The goal is to harmonize the views of industry participants, auditors and valuation specialists within the scope of the Financial Accounting Standards Board's ASC 946, Financial Services—Investment Companies (including private equity funds, venture capital funds, hedge funds and business development companies).
"This guide will provide these professionals with a common language understanding about how to value portfolio company investments," said AICPA senior manager Yelena Mishkevich in a statement.
This guide explains accounting and valuation issues such as unit of account, transaction costs, calibration, the impact of control and marketability, and backtesting to help investment companies deal with the challenges of estimating the fair value of their investments. It can also be used by non-investment companies, such as corporate venture capital groups or pension funds, although it doesn't deal with them specifically. The guide includes 16 case studies that can help guide readers through various types of scenarios encountered by investment fund managers, valuation specialists and auditors.
"Professional judgment plays such an important role in the valuation of private equity and venture capital investments, and, as a result, it is one of the core concepts addressed and illustrated throughout the guide," stated Mark O. Smith, AICPA senior manager of forensic and valuation services.
The guide was developed by the AICPA PE/VC Task Force, which includes members from the private equity and venture capital sector, along with auditors, valuation practitioners and AICPA staff.


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Where to find the best halal restaurants in Brisbane and the Gold Coast
With a rising number of Muslims calling Queensland home, as well as the state welcoming many others for their holiday, one thing is for certain, halal dining options are on the rise. If you're looking for the best in the business, we've done the fork work to bring you the best halal restaurants in Brisbane and the Gold Coast.
Best halal restaurants in Brisbane
Black Hide Steakhouse, Brisbane CBD
You'll get a genuine taste of old-world elegance at the upmarket Black Hide Steakhouse in one of Brisbane's most historic buildings right in the city centre. Designed in classic revival style, with soaring ceilings, The Treasury Hotel, built from locally quarried sandstone and tuff was completed in 1928. The menu is equally local-focussed, with 100% halal certified prime Queensland organic, wagyu and Angus steaks that come served with crispy wagyu-fat roasted potatoes and your sauce of choice.
Muslim friendly
Eat Street Markets, Northshore
Join the throngs of locals at sundown on Friday or Saturdays, or from lunchtime on Sunday at Eat Street Markets with its 'alleyways' of shipping container stalls. There are more than 70 vendors selling a smorgasbord of international food; from Japanese and Turkish to Asian street food. Choose your food and grab a chair in front of one of three stages for free entertainment, including rock and pop, jazz and family-friendly events. Eat Street's website will guide you to stall holders with halal options.
Muslim friendly
MADO, South Brisbane
At the quieter end of South Bank Parklands, Mado is perfectly situated for a break if you're enjoying swimming at Streets Beach or exploring South Brisbane's cultural precinct. Service is welcoming and the décor vibrant, with Anatolian carpets and wall hangings and a belly dancer, Friday, Saturday and Sunday as entertainment. Try the Turkish manti – small dumplings filled with spiced ground beef tossed in a caramelized tomato sauce served with homemade garlic and mint yoghurt, the best Turkish pide in town, or tender smoky, meats charred over charcoal.
Mu'ooz, West End
West End, with its markets, cafes, and bookshops is one of Brisbane's more colourful inner-city suburbs and is just a short walk from neighbouring South Brisbane's Queensland Art Gallery and Gallery of Modern Art (QAGOMA) the Queensland Museum and South Bank Parklands. Friendly and cozy, Mu'ooz, a not-for-profit social enterprise restaurant serves traditional northern African cuisine; slow cooked meats like lamb qulwa or derho zigni – a favourite spicy beef or chicken curry flavoured with the much loved berberé and designed to be sopped up with the soft, spongy injera pancakes. Finish with an African donut drizzled with orange syrup or semolina cake and a cup of traditional Eritrean coffee.
Best halal restaurants on the Gold Coast
Shiraz Authentic Persian Restaurant, Surfers Paradise
Go with a healthy appetite and a few friends or family– this Iranian favourite in the middle of Surfers Paradise is famous for its generous portions, as well as warm hospitality. The menu has something to please everyone – from a selection of dips served with warm flatbreads to share, to their signature lamb shank with 'green' rice and plates of smoky chicken, beef or lamb kebabs served with saffron rice. Finish a meal (if you have room!) with 'Chai O-Shirin' – Persian tea served with a selection of biscuits.
Charis Seafood, Southport
There are few more simple pleasures than having fish and chips overlooking the water (so much so we even have a blog post about it). Bordering Harley Park, marked by rows of stately Norfolk pines right on Southport's broadwater, Charis is a local institution. Put in your takeaway order and set up at one of the picnic tables or spread a blanket on the grass to enjoy an alfresco lunch or dinner. If you're here at 1.30pm, you'll be able to catch the unofficial wildlife show as squadrons of hungry pelicans gather to be fed by Charis staff.
Muslim friendly
Catch at The Hilton, Surfers Paradise
High above the vibrant centre of Surfers Paradise with uninterrupted ocean views is the aptly named Catch at The Hilton. Sink into a banquette and order from the modern Australian menu that includes entrees like tempura barramundi soft taco with onion pickle, chipotle mayo, avocado and corn salsa or soft pillows of potato gnocchi with asparagus, zucchini, corn, parmesan and truffle. Fish mains include favourites barramundi, salmon and kingfish. Put aside your diet for desserts like churros with white chocolate, raspberry crispies, Chantilly cream and coconut – you can always walk it off on the beach afterwards.
Muslim friendly
NightQuarter Markets, Helensvale
More than 120 speciality food purveyors have set up shop in shipping containers at the Gold Coast's vivacious creative playground NightQuarter, where locals and visitors come meet, eat and listen to music under twinkling lights. Start off your evening by wandering the high energy Hawker Street, with cuisine from all over the world. Then move on to Tapas Street, with a dozen different vendors offering plates that are perfect for sharing. Sweet tooths can take an organised 'dessert tour' of the markets, or book in for high tea.
Muslim friendly
Looking for more food ideas in Brisbane and the Gold Coast, we've got you covered with:
- Brisbane's most Instaworthy brunch spots
- The Gold Coast's best vegan eateries
- Best breakfast spots on the Gold Coast
- Brisbane's best burgers


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So you all should love it when I have absolutely nothing to blog about, as that usually results in me dredging up some embarrassing piece of nonsense that I wrote/drew/acted in the distant past in order to fill the day's blog post quota.
Example: Today's post, in which I scan the pages of a children's story I wrote in first grade. It was a coo story, because I was all about the coos back then. I wrote/illustrated the story in first grade, but in 1997 (fourth grade? Third grade? I dunno), I made a "fancy" copy of it where I typed out everything in Word and re-drew the illustrations to make it look better than the original first grade scrawl. I also made all the "official book info" by copying almost directly from the inside page of a Babysitter's Club book.
So enjoy. (Note: their names are pronounced "heeb," "OH-lay," and "peeb")
Plots are hard when you're a first grader.


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Dimmitt High School
Class Of 1969
Sharla Sanders Mays
Profile Updated: August 22, 2014
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|Customer Service for American General Life Insuran
| son: Shayne Mays, born 1973, is an officer with the Randall County Sheriffs Dept and has 2 children
daughter: More…
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Aug 23, 2014 at 5:19 PM
Linda, my e-mail is email@example.com; send me your e-mail address so I can tell you the latest events of my sorted life. I will try to do better about keeping in touch.
Sharla Sanders Mays updated her profile. View.
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Road Test: Jaguar XJ L 3.0d Autobiography
An evolutionary refresh for a revolutionary part of Jaguar's product history, explains Alex Grant.
SECTOR: Luxury PRICE: £79,920 FUEL: 49.6mpg CO2: 149g/kmAlthough the luxury sector is dominated by the ubiquitous S-Class and generation-after-generation brand loyalty to Stuttgart, Jaguar's XJ saloon has found some illustrious customers in its near 50-year history. Merc might transport high-flying businessmen, but for royals and Prime Ministers it's the big cat that's got the cream.
Jaguar has, of course, gone through a bit of a turnaround over the last decade. In the eight years since the latest XJ adopted the brand's curvaceous new family design, its siblings have established themselves in fleet. A family now spanning the compact executive XE, the F-Pace SUV and aspirational F-Type sports car, each making what was once new-age Jag design familiar. Yet, somehow, the XJ's plunging rear roofline and upright rear lights have lost none of that curbside presence in the years since.
There hasn't been any need to perform radical surgery here; the mid-life facelift introduced a reshaped nose with a more upright grille and LED headlights separated to look like twin lamps. Both are subtle nods to the original XJ of 1968. For a car measuring just short of 5.3 metres end to end in long-wheelbase form, as tested here, it looks impossibly athletic, somehow hiding its sheer size among the curves.
All of which is a hint at its USP. The XJ is largely made from aluminium, which means it does just as effective a job of hiding its bulk when it's on the road. It's not a car that wills you to drive quickly, but it covers ground effortlessly and corners like a much smaller car. Yet, despite the 20-inch wheels and slither of rubber wrapped around them, it doesn't feel like a four-seat sports car on poor surfaces.
Jaguar only offers one diesel engine in the XJ, a 297bhp twin-turbocharged V6, up 25bhp on the old one and now with a more accurate fuel injection system to improve fuel economy. The eight-speed automatic gearbox helps, but it's fairly easy to get over 40mpg driven gently, but there's plenty of straight-line performance available when needed too. But, at 149g/km, its closest rivals are way ahead on CO2 emissions.
Autobiography versions were also introduced with the refresh; it's a top-of-the-line trim level exclusive to the long-wheelbase model. Jaguar interiors can be a bit plasticky compared to rivals, but the XJ feels a cut above with its lashings of soft leather and stainless steel trim pieces. Rear occupants get 10.2-inch screens and tray tables, plus individually-adjustable seats, but the XJ falls down a little when it comes to leg or headroom. You get more space in the German luxury saloons.
This is a segment that moves fast, the part of the market where new technology breaks cover. New versions get the latest InControl infotainment system and semi-autonomous traffic jam assistance which can bring the car to a complete halt – good news for urban chauffeur fleets – and the Land Rover-esque low-speed cruise control for slippery surfaces is clever too. But technologically it's a generation behind the new 7 Series and even the soon-to-be updated S-Class. Jaguar also doesn't offer a plug-in hybrid – rapidly becoming a prerequisite part of a luxury car line-up.
However, this is a thoroughly likeable car; great to drive, striking to look at and something a little different to the scaled-up executive saloons. In a segment which moves quickly, Jaguar hasn't fallen behind the curve yet.What we think:
The XJ feels more tuned towards the driver than rear-seat passengers – whether that's a positive or negative depends on your point of view. There's more space elsewhere in this segment, but for sheer presence and driving dynamics the big Jaguar is hard to fault.
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