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Carol日前回巴西時接受了Terra獨家專訪Carol Trentini posa para Reinaldo
Lourenço e fala com exclusividade ao Terra,tfs的ckgirlbr幫大家翻成了英文,
She has captured the fashion world with dedication and without egocentrism. From the humble girl of Panambi, small town of Rio Grande do Sul, to prestigious top model, Carol Trentini, at 21, from Way Models agency, has already been on the cover of big important magazines and has posed to three big photographers: Mario Testino, Patrick Demarchelier and Steven Klein.
This month, for the second time consecutive, the model came to Brazil to fulfill her professional commitments. She posed to the Forum ad campaign with the king of porno fashion, the photographer Terry Richardson, and now she's in the campaign of the new clothes line that the designer Reinaldo Lourenço is developing for C&A.
Muse of the powerful Anna Wintour, US Vogue editor, the Brazilian top model is also on the cover of the last issue of the publication, with other important models and is pointed as one of the most promising models of the new generation. To have an idea, the model is in every issue of the magazine. She was the only model to seat at Anna's table in MET gala ball and has her name underlined alongside other models in the exposition about models that is on Metropolitan Museum, in NYC.
With a vintage Mickey t-shirt, Redley short jeans, Balmain jaguar sandals and Rolex watch, Carol Trentini took a break in her schedule to talk with exclusivity to Terra and give some details of the fashion world.
Terra: You're with a casual look. Is this the kind of clothes you like wearing?
Carol Trentini: I like comfort when I dress myself. I almost never buy clothes; I've earned everything I've got.
Terra: How was to work with Terry Richardson for the first time?
CT: It's funny, Terry and I are neighbors in New York. I always meet him at the supermarket or when he's walking with his dogs, and we never worked together. I think our first professional experience was very interesting. The pictures of this work are beautiful, a little sexy that is Terry's typical style and it is what everybody from the fashion world loves.
Terra: Is that true that one of your aims is to fight for more works in Brazil?
CT: Despite my busy schedule abroad, I want to work more in Brazil. I'm living a new phase of my career. I've already learned everything about the profession and I know very well how to manage it. In this case, I'm being able to choose better the works I'm going to do and Brazil is always in my plans.
Terra: From the humble girl of Panambi to top model in New York. Has that transition influenced in any way your personality?
CT: I've always been shy and very simple. Despite achieving what I've got until now, I haven't changed my personality. I think I've grown up in the middle of that transition.
Terra: Have you passed through any kind of financial difficulty?
CT: My mother has always worked very hard and despite all difficulties, food has never been lacking on our table. When I was invited to become a model, I embraced the cause and told myself: I'm going to win and help my family. And that's what happened. I always thank the opportunities I had and I got happy to be able to give something in return with my family.
Terra: You lost your father very early. In any moment you missed a paternal figure?
CT: My mother always knew how to execute both roles very well. Actually, I have a godfather that took care as his daughter and in this case I transferred my attentions to him.
Terra: You were discovered by a scouter. How as your reaction when you were approached to be a model?
CT: I was very frightened, because I didn't know very clearly how it was to be a model. I used to live in a small city, with just a little fashion information and I didn't understand very well what that was. I've always been very close to my mother and she motivated and supported me.
Terra: The first work we never forget. What was yours?
CT: I came to São Paulo in an excursion with a bunch of girls and I ended up staying in the agency apartment. My first work was a showroom of Carlota Joaquina. In that time, Dudu Bertolini used to work there and I got 130 reais (about 65 dollars) in exchange of clothes (she laughs). I took two tops and got very happy.
Terra: Have you been rejected to a work?
CT: I don't like that work, rejection. I think that getting a "no" is common everywhere. I've already received many "nos" and I still receive some. In this case, it's very important to work your self-stem.
Terra: Have thought of giving up of your career in any time?
CT: I've already thought that, but I was strong. In my first week in São Paulo, I cried in the laundry of the apartment, because I didn't know the city and anything about the fashion world. But I learned how to deal with the difficulties and said: either I return to Panambi and go back to my studies or I really get into this. I chose the latter.
Terra: An unforgettable work.
CT: I owe a lot to Steven Meisel (photographer). It was him who taught me everything I know today. Another important work was my first one for US Vogue that I'll never forget of.
Terra: Talking about that, what's the impression you have on US Vogue editor, Anna Wintour that has the fame of being tough?
CT: We both have always got along with each other. She's very professional, I even admire her for being the woman she is and mostly for the important role she plays in the fashion work.
Terra: Who are the most important photographer you have worked with?
CT: David Sims, Patrick Demarchelier that is so sweet, Steven Klein, Mario Testino, now with Terry Richardson and Irving Penn that is a legend of photography. He draws me before shooting me and say beautiful words like "you're my heroin of the photo".
Terra: What's your expenditure dream?
CT: I hate airports and I'd like to have a private jet. Who know someday (she laughs).
翻譯by tfs-ckgirlbr
- Jun 01 Mon 2009 21:04
Terra獨家專訪Caroline Trentini


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Washington, DC, elites might obsess over Russia conspiracies but Middle America doesn't care that much.
The media can't seem to move past their own hysteria over Russia conspiracies. But can the Democrats hope to use outrage and fear over Russia conspiracies to win victories next November? Increasingly, even progressives are answering that question with a "No." Average voters don't care enough. The media and political activists have not been able to get their own outrage over and belief in "collusion" to spread among normal people.
The Guardian published an editorial entitled, "Democrats beware: the Trump-Russia inquiry isn't the path to power."
At first sight, polls seem to indicate that the Democrats do have a potential winner in the Russia investigation. When White House spokeswomen Sarah Sanders said that "no one cares about this issue", the Washington Post claimed to prove her wrong by showing that "nearly half – 49% – of Americans believe Trump tried to interfere with the Russia investigation in a way that amounts to obstruction of justice". Indeed, other polls confirm that a plurality of Americans believes Trump "acted improperly when it comes to any alleged coordination between the Russian government and Donald Trump's 2016 presidential campaign." But that was not Sanders' point!
While Sanders definitely exaggerated when she said that no one cares, she did have a point when she elaborated "it's certainly not the thing that keeps people up at nigh.". Only 35% of Americans said that the investigation into whether the Trump campaign colluded with Russia should be a "top priority" for Congress. Another 18% says it should be "an important, but lower priority." While this amounts to 53% overall, even that number was lower than that for any of the other seven issues included, with the exception of Trump's much desired wall on the US-Mexico border.
In other words, the Russia-Trump collusion story might be the talk of the town in Washington, but this is not the case in much of the rest of the country.
The views expressed in this opinion article are solely those of their author and are not necessarily either shared or endorsed by EagleRising.com


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Efficient Diary Pro is an elegant, easy-to-use and powerful electronic diary software package. With its unique and powerful flash full-text search technique, you can simply enter a word in the diary to quickly find the corresponding entries! The product has a strong edit function similar to that of MS-Word. A happy life can't be without Efficient Diary!
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Christmas Illuminations at Mount Vernon
Where: George Washington's Mount Vernon
3200 Mount Vernon Memorial Highway, Mount Vernon, Virginia 22121
When: Saturday, December 17, 2016, 5:30 pm – 9:00 pm
Details: Join Mount Vernon for an evening of family-friendly fun and fireworks choreographed to holiday music, with fireworks beginning around 9 p.m. Take a stroll through the estate while being serenaded by local choirs, visit with re-enactors from the First Virginia Regiment in winter encampment, and learn 18th-century dance moves from costumed guides in the Greenhouse. "George and Martha Washington" will also be on site to greet Mount Vernon's holiday guests.
Watch as colonial artisans demonstrate the 18th-century process of creating chocolate. Keep toasty by warming up by a bonfire, visiting the blacksmith shop, or enjoying some hot chocolate or cider. Additional tasty food will be available for purchase. Still haven't found the perfect holiday gift? Take some time to complete any last minute gift needs at the Shops at Mount Vernon, open late during Christmas Illuminations.
Christmas Illuminations takes place rain or shine.
Cost: $34 adult/$24 youth with Mansion tour. $30 adult/$20 youth without Mansion tour.
Click here for more information.


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What is in The Mechanics of Art? In a work of art, can the audience's perceived meaning differ from the artist's intent? Does the process of collaborative art-making inspire liberation or impose restrictions? Art historian and ArtCRS co-curator Kathryn M Davis moderates a one-afternoon series of rapid-fire panels that highlight the process of repairing "stalled" artworks while unpacking larger ideas associated with cooperation and failure in the mechanics of art which is making of a work of art. Featuring artists Mathew Matthew Chase-Daniel, Jamie Hamiliton, Cheri Ibes, Christopher Johnson, Don Kennell, Eve Andree Laramee, Nina Mastrangelo, Stacey Neff, Willy Richardson, Michael Schippling, Sydney Cooper, Dylan McLaughlin, Judy Tuwaletstiwa, and Jerry Wellman, with an introduction by ArtCRS creator Susan Begy.
Saturday January 10, 2015
Center for Contemporary Art
1050 Old Pecos Trail
Santa Fe, New Mexico
2Pm ~ 4:30PM


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Sold Out
Whether you are furnishing a Country Estate, Cape Cod Cottage or Elegant Townhouse there is a Lambswool throw to suit each and every environment.
Color choices come in soft shades of Gray, Beige, Lilac and Sage.
Seen here in Beige.
Approx Size incl. fringe 55" x 72" (w x l)
This product is manufactured by Abraham Moon & Sons Ltd who have been producing quality wool products at their mill in Yorkshire, England since 1837. This is one of the last remaining vertical mills in England and it has been operating since the end of the Industrial Revolution.They are World Leaders in dyeing, spinning, weaving, blending and finishing, producing beautiful products that you can cherish for a lifetime.


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If your non-franchise dealership uses Autosoft's Sales and F&I (SFI) platform but employs QuickBooks rather than the full Autosoft DMS Accounting module, Autosoft's QuickBooks integration will allow you to easily transfer vehicle sales data from your Autosoft SFI platform to QuickBooks.
If your dealership uses the NIADA chart of accounts in QuickBooks, your Autosoft QuickBooks integration setup should be relatively simple. After you've purchased the QuickBooks integration from Autosoft, Autosoft will turn on the integration for your dealership and will populate your system using default general ledger accounts and vehicle sales formulas based on the NIADA chart of accounts.
These accounts and formulas are set up with the appropriate formulas and account names and numbers to comply with NIADA, so you will only have to make adjustments if you choose to edit, remove, or add accounts or vehicle sales templates tailored to your dealership's policies and usage.
All QuickBooks integration setups are performed within Autosoft DMS's Setup application under the Accounting and Sales tabs. It may be best to begin with the Sales setups affected by the QuickBooks integration and then move to the Accounting setups.
The Accounting tab's menu is comprised of two options: General Ledger and Vehicle Sales.
General Ledger
Clicking the General Ledger option in the Accounting tab's options menu opens the General Ledger Account Search Screen. For dealership's that employ the NIADA chart of accounts, upon activation of the QuickBooks integration, the General Ledger page of Accounting setup is automatically populated with all the general ledger accounts used by NIADA. Accounts are listed by Account Type, Account Name, Account Number, Account Description (this field will be blank for all pre-loaded general ledger accounts, as it's not required by QuickBooks), and account Status.
You can sort the list by column content by clicking the column heading by which you wish to sort. For example, clicking the Account Number column header once sorts the list by account number in ascending order. Clicking the Account Number column header a second time sorts the list by account number in descending order.
The General Ledger Account Search screen also allows you to search for an account using the Search bar. Simply type a search term into the Search bar and click Search.
The search results populate the screen. To clear a search, click the gray X in the Search bar.
Single-click an account from the list to open its quick-look card, where you can view the Account Type, Status, Account Number (if entered), and Account Description (if entered). The quick-look card also contains the Edit Account button, which opens the account's General Ledger Setup pop-up in edit mode, allowing you to edit the information entered for the account.
You can also open the general ledger account's General Ledger Setup pop-up in edit mode by double-clicking the account from the list.
The General Ledger Setup pop-up will open. Here, you can make any necessary adjustments to the Account Type, Account Name, Account Number, or Account Description (optional field).
You can also set an account as "Inactive" here by clicking Set Inactive. (Inactive general ledger accounts do not appear in the General Ledger Account Search screen unless you click the Show Inactive toggle to include inactive accounts in the list.) After clicking this button, the button label changes to Set Active; clicking this button reactivates the general ledger account.
If you make any changes in an account's General Ledger Setup pop-up, be sure to click Save and Close to save your changes.
You can add accounts to this list using the Add Account button in the upper-right corner of the screen. For full directions, see the Knowledge Base article, "Adding General Ledger Accounts for the QuickBooks Integration."
Vehicle Sales
Clicking the Vehicle Sales option in the Accounting tab's options menu opens the Vehicle Sale Search screen. For NIADA chart of accounts users, this screen will be preloaded with the used vehicle sales templates you'll use most frequently, but you can use the Add Setup button in the upper-right corner of the screen to add any additional templates as needed. (See the Knowledge Base article, "Adding a QuickBooks Vehicle Sales Template," for full step-by-step directions.)
Each vehicle sales template is listed on the screen by Deal Type, Inventory Type (always "Used"), Class, Sale Account, and Status. You can sort the list by column content by clicking the column heading by which you wish to sort.
The screen is searchable. Simply type a search term in the Search bar and click Search, and the list will be limited to the results that meet your search criteria. Clear a search by clicking the Clear button; the full Vehicle Sale list will reappear.
To view a vehicle sales template, double-click the template from the list. The template will open, listing all the associated general ledger accounts and the formulas used to calculate values posted to those accounts.
The formulas are created for you and use figures from sales deals and vehicle records. Most formulas do not require adjustment (Hard Pack and Other custom formulas may need to be added, as these items differ from dealership to dealership), but you can adjust them by clicking the Edit toggle in the upper-right corner of the screen and using the small blank fields to the right of each formula.
You can select +, -, x, or ÷ from the menu that opens. You can then add a preset formula from the data dictionary by clicking Table and selecting a field from the Deal or Inventory table lists; click Formula Builder to create a formula from scratch. You have the option of creating a simple formula with dollar figures and table items, or using the percentage formula type to create a forumla that takes a percentage of dollar figures and/or table items. Click Save to save any changes you've made. (See "Creating Formulas for Vehicle Sales Templates," for full instructions.)
If you make any erroneous adjustments to templates, you can click the Reset to Defaults button in the upper-right corner of the screen. The system will confirm that you wish to reset the current template to the account and formula defaults. Click Confirm to continue. Any changes you made to the template will be undone, and the original Autosoft-created template will appear.
|Warning: If you've made previous changes to a template and saved them prior to the erroneous formula adjustment, those changes will be lost when you use the Reset to Defaults button. Before making changes to any preset templates, be sure you understand the formula change and any previous changes that have been made so you can recreate those changes if a reset is necessary.
You can also mark a template as "Inactive" if it's no longer relevant. Simply click Set Inactive in the upper-right corner of the template screen. (Inactive vehicle sales templates do not appear in the Vehicle Sale Search screen unless you click the Show Inactive toggle to include inactive templates in the list.) You can easily reactivate a template by opening the inactive template and clicking the Set Active button in the upper-right corner of the template screen.


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The Research Accreditation Panel consists of independent members, representatives from government departments, and representatives from the devolved administrations.
Independent Members
The Research Accreditation Panel is chaired by independent member Professor Paul Boyle CBE. Professor Boyle is currently Vice-Chancellor of Swansea University and was previously President and Vice-Chancellor of the University of Leicester from 2014 to 2019. Prior to that, he was Chief Executive of the Economic and Social Research Council from 2010 to 2014 and President of Science Europe, representing over 50 European funding agencies. Paul is Chair of the Scottish Science Advisory Council, which is Scotland's highest level science advisory body; a Board Member of Universities UK, who provide leadership and support to executive heads of 133 UK University institutions as well as Chair of their Research Policy Network; Vice-President of the European University Association which represents over 800 universities in 48 countries; a Council Member and Trustee of the Association of Commonwealth Universities, which has over 500 member institutions in 37 Commonwealth countries and an 'impact champion' for the United Nations HeForShe global solidarity movement for gender equality.
Independent member Professor Chris Dibben is Chair in Geography at the University of Edinburgh, specialising in health and environment. He is also Director of the Longitudinal Studies Centre Scotland, Principal-Investigator on the Census & Administrative Data Longitudinal Hub and Director of the Administrative Data Liaison Service. Chris also leads the Scottish Centre for Administrative Data Research hosted by the University of Edinburgh.
Independent member Tricia Dodd is a freelance statistical consultant working mainly with national survey organisations, charities and government departments. She previously held a number of Senior Civil Service positions within the Office for National Statistics including: Chief Methodology Officer at ONS (2016-2018), Deputy Director responsible for Crime and Regional Statistics, ONS (2013-2016) and Deputy Director responsible for Health Statistics and Life Events, ONS (2011-2013).
Independent member Dr Andrew Garrett is Executive Vice President-Scientific Operations at ICON Plc. Between 1995 and 2016 he was Vice President-Clinical Analysis and Reporting Services at Quintiles, Inc. He has previously served on the Board of Directors at Cenduit LLC (2015) and the Administrative Data Research Network (2014-2017). Between 2017 and 2019 he was Chair of the Royal Statistical Society's Data Science Section and is a member of the UK Data Forum.
Independent member Sarah Mathieson is the Head of Research and Knowledge at the Institute and Faculty of Actuaries. Prior to this appointment she was the Head of Proposition Management (Trustee) at AEGON UK (2010 to 2011) and a Corporate Finance Manager and Business Development Actuary at the Pearl Group (2007 to 2010).
Representatives from Government Departments
Kevin Fletcher is the Chief Data Officer and Chief Economist at HM Revenue & Customs (HMRC). He has previously held a range of Senior Civil Service roles within HMRC including leading teams doing policy, strategy and compliance analysis to large operational teams with operational delivery responsibilities across the UK.
Emma Gordon is Director of the Strategic Hub for ADR UK. Emma joined ADR UK from HM Treasury, where she led the team supporting government economists and social researchers across government. Prior to this, Emma was Head of Health Analysis at the Office for National Statistics (ONS).
Sarah Henry represents the National Statistician on the Panel. Sarah is the Director of Methods, Data and Research at the Office for National Statistics. She was previously Head of Intelligence and Performance at Manchester City Council.
Paul Lodge is the Chief Data Officer for the Department for Work and Pensions. Paul was previously responsible for the identification and development of new capability to address the cyber threat to the UK in the National Cyber Crime Unit (NCCU) at the National Crime Agency.
Neil McIvor is the Chief Data Officer and Chief Statistician at the Department for Education. He was previously Deputy Head of Profession for Statistics, and temporary Chief Data Officer at the Department for Work and Pensions.
Representatives from the Devolved Administrations
Siobhan Carey is the Chief Executive and Registrar General at Northern Ireland Statistics and Research Agency. Prior to taking this role in 2016 she was Chief Statistician and Head of Profession at the Department for Business, Innovation and Skills (2011-2016).
Roger Halliday is the Chief Statistician and Data Officer at the Scottish Government. Prior to being appointed to this role he was an analyst at the Department for Health focusing on using evidence to influence decision making around health.
Glyn Jones is Chief Statistician at the Welsh Government. He has held this post since 2013. In this role he is responsible for the statistics produced by Welsh Government and is the senior statistical advisor to Welsh Ministers. He is also the co-Director of Administrative Data Research Wales, part of the wider ADR-UK partnership.


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Blondie Meets the Boss (1939)
Directed by Frank R. Strayer / Frank StrayerGenres - Comedy | Sub-Genres - Domestic Comedy, Screwball Comedy, Romantic Comedy | Run Time - 75 min. | Countries - United States | MPAA Rating - NR
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Synopsis by Hal Erickson
This second entry in Columbia's new "Blondie" series is every bit as delightful as the first. When Dagwood Bumstead (Arthur Lake) heads off for a long-awaited fishing trip, his loving wife Blondie (Penny Singleton) assumes Dag's duties at the offices of J. C. Dithers (Jonathan Hale). Unfortunately, our hero finds himself in a compromising position with pretty stranger Dottie (Dorothy Moore), endangering both his job and his marriage. The film's highlights include an energetic jitterbug contest and a terrific variation on the old "Any husband who's expected home should leave right now" gag. Blondie Meets the Boss was heralded by a specially filmed trailer in which the Bumstead's son Baby Dumpling (Larry Simms) thanked the audience for the excellent response to the first Blondie picture and inviting the viewers to come back for more (which they did-28 times!)
office, employment, jitterbug, trip, problems, fishing, husband


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With the continued development of the industry, technology leaps and bounds, the application of the vigorous promotion of LED light efficiency is also increasing, the price continues to lower. The emergence of a new modular tube core also allows the power of a single LED tube (module) to continue to improve. Through the continuous efforts of research and development, new optical design breakthroughs, the development of new lights, product single situation is also expected to be further reversed. Control software improvements, but also make the use of LED lighting more convenient. These gradual changes reflect the bright future of LED light-emitting diodes in lighting applications.
LED is called the fourth generation Light source, with energy-saving, environmental protection, safety, long life, low-power, low heat, high brightness, waterproof, micro, shockproof, easy dimming, light beam concentration, simple maintenance and other characteristics, can be widely used in various indicators, display, decoration, backlight, general lighting and other fields.
LED Advantages: Electro-optical conversion efficiency is high (close to 60%, green environmental protection, long life (up to 100,000 hours), low working voltage (3V or so), repeated switch lossless life, small size, less heat, high brightness, rugged and durable, easy to adjust light, color diversity, beam concentration stability, start without delay;


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Tag Archives: cocktail
Here's a look at the latest bar trend: Cocktails on tap
There was a time when drinking a cocktail to meant imbibing something sickly sweet and/or girly (like a Cosmo) or far too much alcohol than my 5-foot-one-inch frame can withstand. So I stuck with my fume blancs, malbecs and cabernets. Well … Continue reading
Posted in Baltimore food and drink, cocktails, Food & drink Tagged baltimore, baltimore sun, bookmakers cocktail club, canton, cocktail, craft cocktails, downtown, federal hill, fells point, fork and wrench, harbor east, mount vernon, of love and regret, warehouse 518, wit and wisdom Leave a comment


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When it's Outland.
Soon after Berkeley Breathed decided to end Bloom County in 1989, the Pulitzer Prize-winning cartoonist launched Outland. And while there were certain…penguin-ish similarities…in the Sunday-only comic strip, Breathed broke new ground with Outland that made it every bit as fresh and unique as Bloom County was before it.
We're collecting the entire run from 1989-1995 in a single book—more than 300 Sundays. The proofs just came in from the printer and we should be okaying it for press by tomorrow. From this point, it generally takes about two months until the book will be on sale in stores and online.
Here's a sneak peak. You might recognize a few of the characters…


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These Are the Buzziest Wellness Buys for Your Home, According to Interior Designers
Jenna Adrian-Diaz・March 20, 2018
It's safe to say that the hero products that graced your Zen den in 2017 aren't going anywhere. You know and love your Himalayan salt lamp for its *literal* good vibes, and that sherpa throw is a hygge essential. Plus, who could forget the urban jungle that is your collection of shower plants?
But with spring afoot, the new class of healthy-home essentials is making itself known through the emergence of a new design ethos. A profound emphasis on meaningful connection to the environment and the sense of self is taking over.
"We're all starting to get a little bit burnt out and trying to figure out how to say no and how to bring things into our lives that really make a difference and impact our space," says Hyphen Interior Designs founder Shelly Lynch-Sparks of the increasing demands of ever-present technology and stress.
"People are looking for ways to connect to each other and places they're in," explains Adam Rolston, partner architect and creative director at INC Architecture and Design, adding, "People are really looking for meaningful, data-based, facts-based experiences that actually induce feelings of well-being."
Here, Sparks and Rolston share their picks for 2018's new class of wellness pad must-haves.
Keep reading for the designers' picks.
Cozy "womb" chairs
Coined by Eero Saarinen, the iconic mid-century modern design (and its subsequent interpretations) is characterized by its enveloping form. "As human beings, think about how we used to shelter in caves; so objects like a chair that can actually encompass you might make you feel contained and safe," says Rolson. "It's a very basic human need to feel enveloped and safe."
Whether you splurge on a seat in Saarinen's fashion or opt for a rendition like the one above, that feeling of security will impact how you feel when you come home each day.
Home fragrance with sentimental appeal
You know how the whiff of a familiar scent can activate the memories and feelings of another time? Positive olfactory memories—and the scents that rouse them—are a new sensorial frontier of home wellness, according to Sparks. "That sort of personalized scent for the home, as soon as you walk in the door, it tells you how to tune out and turn down and also be present in your own home. And it's also a calming force. It helps your nerves."
It could be a candle that reminds you of cozy nights on the couch with your SO, or an essential oil blend reminiscent of the one your favorite yoga studio diffuses, but by integrating a meaningful scent into your home, you're sure to elevate your mood and your space.
Comforting weighted blankets
Blankets for days is the outlook for the year ahead, and those of the weighted variety bring another dimension of anxiety-busting sensory appeal. "As human beings, we're a pack animal," Rolston explains. "We love to huddle together, yet loneliness is at epidemic crisis level in our culture." The weighted blanket actually does provide a psychological and physiological effect on your body helping you to calm.
Layer under your comforter or mix and match one to go with your favorite throws, but consider the an investment towards your next-level hygge den.
A place to put your brain on 'do not disturb'
You won't exactly be running to the store for this one, but you'll likely be taking it into consideration as you rearrange your furniture next time around. No matter how much you love your besties' texts or your curated Instagram feed, technology can get a little bit overwhelming sometimes. That's why Sparks advocates for a relaxing space in the home that allows you to just be. "We really want to take over and for you to experience what's happening inside the space and let everything else go. We'd rather that be the overwhelming experience than for you to forget about your day," Sparks explains. "Think about things that are going to make a small impact but also feel good."
Whether it's a spot you keep clutter-free to roll out your yoga mat or a decked-out meditation space complete with pillows and blankets, taking a spot and making it your own will enable you to always come back to your center.
In the mood to give your apartment the total home makeover treatment? Here's how to do it, even with a super-strict landlord. While you're at it, flex your green thumb with these low-light-friendly plants.
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Hey there, ports-fans! Sometimes we like our games with a hefty dose of nostalgia and familiarity, and there is nothing wrong with that. Now I wouldn't call Yoshi's Woolly World a port exactly, but it sure feels a lot like Yoshi's Island on the SNES, just newer. And you know what? It felt great! Should you be in possession of a WiiU, this has got to be on your 'must have' list. Don't just take my word for it either; we're going to pick this apart so you know just what to expect.
First of all, you'll notice lately that Nintendo has a bit of a fetish for arts and craft supplies. Perhaps the success attached to Paper Mario spurred something that led to more Paper Mario, then Kirby's Epic Yarn, and more recently, Kirby and the Rainbow Curse. That being said, Yoshi's Wolly World is gorgeous. The tiny fuzzy fibers make Yoshi and friends look like real wool. Backgrounds and environments are made of fabric, sequins, buttons, and of course, yarn. The plushy path bends under your adorable feet while tiny fishing lines hold platforms aloft above. Nintendo is managing to achieve something weird wherein they make unrealistic creatures look hyper realistic, by building them out of real world materials. This is why Kirby got the claymation treatment in his recent game. This is an art choice that makes an insane amount of sense to me. In this way, we can avoid accidentally getting a game with a grotesque photo realistic, scaly yoshi-saurus by hopping right over the uncanny valley.
There has been an immense amount of work for the audio of Yoshi's Wolly World, the score is fantastic and cheery and nearly every single stage has it's own original song. Very little is reused, and to their credit it further breathes life into each stage. If I had a slight complaint, it's that some of Yoshi's vocals seem a bit more grating and obnoxious than before. Here's a taste of the audio in store; this certainly gave me flashbacks to Yoshi's Story on the N64.
Each stage does a great job at introducing or using a new element somewhat unique to it. One of my favorites was a curtain rail stage where you cling to a set of drapes like an unsupervised house cat and slide down the rail much like a mine-cart level. Each stage brings something different that affects the layout and puzzles hidden inside. The object of each level is to get from the beginning to the end. This is a fairly simple task that isn't too difficult overall. Most stages are simple enough in design to the point where you will rarely be halted by an obstacle. Slowed down, certainly, but not dead-stopped. This is where the game feels as though it may be designed for people new to platforming. The challenge for us veterans, however, is in discovering and uncovering collectibles.
In order to complete each stage 100% you must collect objects hidden within. There are five skeins of yarn, five flowers, twenty stamped gems, and you need to finish the level with full health. Objects are hidden well, but not so well as to be nearly impossible to find.
The flowers and health bonuses are just for completion bonus. Gems, however, are certainly worth picking up. You can use these gems as currency later, and disguised among them are stamps that can earn you new stamp images for the Miiverse. They are very reminiscent of the red coins from previous Mario-world platforming titles. Many of them do not seem special until you collect them. The skeins of yarn are the main objective to look for. Collect all five in any given level and you rescue a Yoshi. He basically serves as a new skin/color scheme to play as. It is entirely aesthetic, but I for one love hunting down these new character skins, as it stands to be a real, easily displayed reward for your efforts. Most of them, quite frankly, are freakin' adorable. There I said it. Everything about Yoshi's Woolly World is cute as hell and it's part of it's charm.
As you progress through the game, you will unlock access to power badges. These are temporary powers that feel like old school cheat codes. They cost gems and last until you die or finish the level, whichever comes first. The powers are of varying degree of useful, including unlimited watermelon seed spitting or invulnerability to fire and lava. These are here to help younger or more inexperienced platformers while being less frequently called upon by you hardcore vets. I did catch myself using one when I was stuck that made secrets more visible and therefore easier to see while I was scrounging for yarn. Overall, hardcore platformer players will likely build up a cache of gems and spend them infrequently.
Ever since the release of the first Yoshi's Island, the titular dinosaur has taken to transforming into inanimate objects to clear certain time sensitive obstacles. They were fun sequences then and they are fun now, but it makes about as much sense as a racoon tail enabling Mario to fly. Every few stages, you may find a door that may or may not be optional. There, Yoshi is transformed and transported to a bonus area that usually conceals a skein of yarn. Motorbike Yoshi zooms along at high speeds, mole Yoshi is back with a digging mechanic better than the old days. Umbrella Yoshi is weird but fun, and controls the way a jellyfish game might: opening and closing catches air and helps you flit about on currents of air. Airplane Yoshi plays like an auto scrolling shoot em' up (my favorite). Lastly is mermaid Yoshi. He is a thing that exists in this game. He is weird, but laughably so, and his sequences reminded me of playing Ecco the Dolphin when I was a kid, as you leap out of the water and perform flips as you collect gems. These stages were mostly just timed scavenger hunts but a quick change of beat as well as a load of fun.
Amiibo support is handled well here too, by the way. If you bought a handful of those neat little figures and don't play as much Smash Bros as you used to, scan them in here. You will unlock a character skin based on your amiibo. Every single one works. I swapped between my Bowser and Mega Man Yoshis quite a bit. Yoshi, and the new woolly Yoshi Amiibo don't unlock anything, but they do give you a little power up. It is a lot like the triple cherry from Super Mario 3D World. A second Yoshi appears and does everything you do, matching your every move. This was a cool but not terribly useful feature.
You can also play Yoshi's Woolly World as a co-op multiplayer experience. It shares many of the same benefits and challenges of previous Mario co-op platforming titles. 2D multiplayer platforming makes the game chaotic and more challenging rather than less so. If you have someone in mind, try it out. I wouldn't try too hard to love this feature if it isn't doing anything for you, though. I make a great team with some of my friends, but in a platformer like this, sessions can devolve into a really negative place quite fast.
If you own a WiiU, I'm willing to assume you have played and enjoyed a lot of previous Nintendo titles. Yoshi's Woolly World is not terribly innovative; rather, it takes what worked from a number of previous titles and reassembles them into something that works better than all of them. This game is paced incredibly well, gradually getting more difficult as you progress and with a bare minimum of forty-eight stages (There are optional bonus levels not counted here) you have plenty of content to explore without being overwhelmingly large. If you like Yoshi's Island/Story then you know what to expect and will enjoy this title. Woolly World is a relaxing but challenging experience meant to be accessible to all skill types. You do not need to psyche yourself up or mellow yourself out to be in the mood for this. You can very easily sit down, digest a few levels, then move on. Grand-pappy Nintendo did it again, guys; get your newbies and veterans alike to sit in for this whenever you get the chance.


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The LORD is with me; I will not be afraid. What can mere mortals do to me? Psalm 118:6
Fear is one of the most common feelings that people have. The range of what people fear is nearly endless. It is common to hear about how people fear things like: spiders, heights, closed spaces, bugs, and other things.
It is not so common to hear people talk about fear of other people. The only fear of people that is heard about, is fear of terrorists. In some countries, that may be a reality of actually happening. In America, the reality of getting hit by a terrorist is extremely slim, yet people fear them.
What is not talked about is the fear of what other people think or say. In that line of thought, many fear what others may think or say about them.
The Psalmist says that since God is with him, he has nothing to fear. The question he asks is what can mere mortals do to him.
Those that fear men, are fearing something that has no real basis. As a believer in the Lord Jesus, one, must take a reality look at what a person can really do.
Just like Satan, no person can do anything to you without God's approval. The worst that a person can really do to another person is kill him, if God allows it. For those who believe in Jesus, that is actually the best thing to happen. When you die, you get to go to heaven and be with Jesus for all eternity with no suffering or pain any more.
The other things that people can do to you while on this earth can make you feel pain, both physical and emotional. Although feeling pain is hard and painful, it is not the end of the world. The pain being felt is only temporary.
You are told that God uses suffering to build you up. It can cause you to turn to God and draw closer to Him. It also allows you to show His true love to those who are against you and hate you.
Do not fear what a person may do to you. Trust God to protect you or be with you through the situation. Man cannot really do anything bad to you, God will use it all for your good and His glory.
I pray today that you will know God's presence in your life; that you will know God's power in your life; that you will know God's love for you; and that God will remove all fear of men in your life.


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Find this book at the nearest libraries. (9.9 MB)
Faculty and employers who work with engineers have consistently identified four areas of deficiency in the engineers' technical writing: clarity, organization, precision and economy. This third edition of the guidebook grew out of efforts to teach engineering students at the University of New Haven to improve their communication skills. David Adams lays out fourteen guidelines and numerous examples that students and faculty can use as common ground when discussing writing.
Publication Date
University of New Haven
West Haven, Conn.
technical writing, clarity, organization, precision, COPE
Subject: LCSH
Technical writing--Handbooks, manuals, etc
Civil and Environmental Engineering | Electrical and Computer Engineering | Engineering | Engineering Education | Mechanical Engineering | Operations Research, Systems Engineering and Industrial Engineering | Rhetoric and Composition
Third edition. Previous editions of this booklet were published by Pearson Education, Inc. Alternate title: COPE: A Technical Writing Guide for Engineers.
Repository Citation
Adams, David J. and University of New Haven, "Clarity, Organization, Precision, Economy: A Technical Writing Guide for Engineers" (2014). Civil Engineering Faculty Book Series. 1.
Publisher Citation
Adams, David J. (2014). Clarity, Organization, Precision, Economy: A Technical Writing Guide for Engineers, Third Edition. West Haven, Conn.: University of New Haven.
Publication Status
Included in
Civil and Environmental Engineering Commons, Electrical and Computer Engineering Commons, Engineering Education Commons, Mechanical Engineering Commons, Operations Research, Systems Engineering and Industrial Engineering Commons, Rhetoric and Composition Commons


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Why Legendary Fashion Photographer Mario Testino Is Embracing Digital Media
Clio winner on memorable ads and building his brand
Current gig Fashion photographer, philanthropist and founder of creative agency MarioTestino+
Instagram @mariotestino
Age 61
What does winning a Clio lifetime achievement award mean to you?
It means a lot to win a lifetime achievement from anyone—but the Clio Awards are particularly interesting, as advertising is such a competitive industry. It's a business that is constantly searching for the new, so it's a big challenge to stay in the fight and to stay relevant. Photographers are changed at the drop of a hat. You have to be constantly questioning yourself and renewing yourself. This award makes it feel like the past 30 years of hard work, and insecurities, and redefining who I am, what I do, and how well I can relate to clients, has been recognized.
What makes a memorable fashion ad? And why is print still an important medium for fashion photography?
To me, a memorable ad is one from which you can remove the brand's name but still instinctively see it belongs to that brand. If I can do an ad for Burberry or Michael Kors, take the name off the image and have people recognize it as a Kors ad or a Burberry ad, that's what makes it successful. Consumers are the ones choosing the way we view fashion photography. I think print provides a richer and less disposable medium; it showcases the magical moments in time that a photographer captures.
Your "towel series" on Instagram, which features models and celebrities dressed in only a white towel, has been very successful. How did that project begin?
I've always worked under other people's editorial guise, and all of a sudden I have this opportunity to portray things my own way. I've always been obsessed with people's bodies, and this was a way of undressing people, but still being able to publish it! I thought it was exciting to do it on a platform like Instagram, which is all about feeding people with new ideas.
Why is a digital presence important to you and how are you continuing to build your brand on social media and online?
I'm always trying to grow and keep making my work exciting, for myself and my audience. When I started using social media, I thought it was good to treat it like a magazine, because I wanted to include my voice in social media, not just my imagery. I find it interesting because it challenges me to combine my creative and commercial sensibilities, having to think of how to not just build better pictures but to establish a wider presence in the digital world.
Your YouTube channel has a lot of behind-the-scenes videos from different projects you've worked on. Why let people into your creative process?
When I started as a photographer, it was hard to learn—there wasn't a school that actually taught you how to become a fashion photographer, largely because such a thing can't exist. You learn it as you go along. I think by showing the behind-the-scenes footage, you're allowing people to learn and see how things are done. Educating and passing on my experience is something very important to me, and it's something I enjoy.
You've shot for nearly every top fashion house. Are there any brands left that you've dreamed of working with but haven't had the chance?
One of my favorite things is having the opportunity to redefine something that has already been defined throughout the years. I'm excited about companies like Ralph Lauren and how it's going through a huge makeover. I like that idea. It's always exciting to relook at brands that are iconic, like Calvin Klein. I remember working with Eva Herzigová back in the '90s when everyone saw her as the Wonderbra girl. I wanted to use her for Calvin Klein, so I did a story of her as a butcher for Face Magazine—to help people see her through different eyes. I love doing that, and I feel that whether it's Ralph Lauren or any other company, having the chance to show people a different perspective is exciting.
How did you get involved in interior design? Is that something you're hoping to do more of?
I find that every photograph belongs within a particular space, and that space needs to be defined before the photograph itself can be taken; I've been defining spaces for the last 35 years. I think taste can be applied to any space by choosing colors, composition and art, so the mixture of all of these elements is so interesting for me. It would be exciting to define new spaces by recreating people's houses or their offices, I have done this for myself over the years and would love to do more commercially in the future.
Tell us about your museum in Lima and how that ties into your philanthropical work.
Growing up in Peru, we thought of ourselves as being so much less developed than the rest of the world. We came to think that we're not at the forefront of the creative fields, or shouldn't have anything to do with them. I thought that by bringing my work to Peru, I could show people that Peruvians can know success and provide a platform where local artists can be celebrated. I also wanted to attract international artists and culture, to allow those who don't have the means to go and discover the world themselves, to be inspired and to be a part of the developing cultural movement happening in Peru right now. It's the first time you've really been able to sense Peru joining the rest of the world in that way.
This story first appeared in the September 12, 2016 issue of Adweek magazine.


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Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes
Contributed by Gerald R. Crabtree
The SWI/SNF complex in yeast and Drosophila is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. The mechanism by which it is targeted to specific genes is poorly understood and may involve direct DNA binding and/or interactions with specific or general transcription factors. We have previously purified a mammalian complex by using antibodies against BRG1, a human homologue of SWI2/SNF2. This complex is likely functionally related to the yeast SWI/SNF complex because all five subunits identified so far (referred to as BAFs, for BRG1-associated factors) are homologues of the yeast SWI/SNF subunits. However, we now describe the cloning of the 57-kDa subunit (BAF57), which is present only in higher eukaryotes but not in yeast. BAF57 is shared by all mammalian complexes and contains a high-mobility-group (HMG) domain adjacent to a kinesin-like region. Both recombinant BAF57 and the whole complex bind four-way junction (4WJ) DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. Surprisingly, complexes with mutations in the HMG domain of BAF57 can still bind 4WJ DNA and mediate ATP-dependent nucleosome disruption. Our work describes the first DNA binding subunit for SWI/SNF-like complexes and suggest that the mechanism by which mammalian and Drosophila SWI/SNF-like complexes interact with chromatin may involve recognition of higher-order chromatin structure by two or more DNA binding domains.
The SWI/SNF complex was originally identified in yeast and is thought to facilitate transcriptional activation of several inducible genes by antagonizing chromatin-mediated transcriptional repression (1–5). The complex has been purified (6, 7) and shown to contain an ATP-dependent nucleosome disruption activity. This activity can lead to enhanced binding of transcription factors to nucleosomal templates (7–9). The complex is 2 megadaltons in size (10) and 8 of its 11 subunits have been characterized: SWI1, SWI2/SNF2, SWI3, SNF5, SNF6, SNF11, TFG3, and SWP73 (4, 11–15).
In mammals, several forms of SWI/SNF-like complexes have been partially purified (16) and purified (17) and shown to have similar ATP-dependent nucleosome disruption activities as the yeast SWI/SNF complex (16–19). They seem to be involved in transcriptional activation by the glucocorticoid and retinoid acid receptors (20, 21), in tumor suppression by interacting with retinoblastoma protein (22, 23), and in integration of HIV by interacting with its integrase (24). The mammalian complexes we purified consist of 9–12 subunits depending on the cell line used for purification (17). Five subunits have been cloned: BAF190 (BRG1, hbrm) (20, 21, 25), BAF170 (26), BAF155 (26), BAF60 (26), and BAF47 (17, 24, 27). They are homologues of yeast SWI/SNF subunits, SWI2/SNF2, SWI3, SWI3, SWP73 and SNF5, respectively.
Recently, a complex similar to SWI/SNF has been purified from yeast (28). This complex (called Rsc) is present at much higher level than SWI/SNF. Mutations of several Rsc subunits produce phenotypes that are distinct from SWI/SNF mutations, indicating that its biological functions are also different (28–30). Rsc is required for yeast mitotic growth whereas SWI/SNF is not. The Rsc subunits identified so far (Sth1p, Rsc8, sfh1p, and Rsc6p) are all homologous to the yeast SWI/SNF subunits (SWI2/SNF2, SWI3, SNF5, and SWP73, respectively). The same four Rsc subunits are also homologous to the mammalian BRG1-associated factors (BAFs) (190, 170/155, 47, and 60, respectively). Thus, in terms of the subunit composition, the mammalian complex is as much related Rsc as to SWI/SNF. In terms of abundance, the mammalian complex is more related to Rsc than to SWI/SNF because it is the most abundant form within the cell (17). Furthermore, BRG1 has recently been shown to be required for the viability of F9 murine embryonal carcinoma cells (31). Thus, in terms of function, the mammalian complex may be more similar to Rsc. The name SWI/SNF for the mammalian complex could be misleading, because the mammalian complex could be a functional homologue of the Rsc complex. Therefore, we will refer to the complex as BAF for Brg1 or brm-associated factors.
A fundamental question regarding the mechanism of action of the SWI/SNF complex is how it is targeted to a specific chromosomal locus. At least three models have been proposed from different studies. In one model, the complex is recruited to the promoter through interactions with sequence-specific transcription factors, such as glucocorticoid receptor (32). In the second model, the complex is recruited through stable association with RNA polymerase II holoenzyme (33). A third model was suggested by the observation that the yeast SWI/SNF complex contains intrinsic DNA binding activities similar to high-mobility-group (HMG) proteins (34). All three models may be correct under certain circumstances, and they may not be mutually exclusive. However, which subunit(s) in the complex interacts with sequence-specific transcription factors, associates with pol II holoenzyme or binds DNA is not clear.
We previously demonstrated that a 57-kDa subunit, BAF57, is present in all mammalian BAF complexes that have been purified (17). In contrast, several other subunits are selectively expressed in specific cell types, suggesting that BAF57 is a core component of the mammalian BAF complexes (17, 26). In this study, we report the molecular cloning of the genes encoding BAF57 from human and mouse. We find that the BAF57 sequence contains an HMG domain and a kinesin-like coiled-coil region. The recombinant BAF57 HMG domain as well as the entire mammalian complex can bind DNA with characteristics similar to other HMG proteins. In addition, BAF57 is a mammalian subunit that has no corresponding homologue in the yeast SWI/SNF complex underlining the possibility that the mechanism of the yeast complex may not be strictly applicable to the mammalian complex.
Purification of Mammalian BAF Complexes and Cloning of BAF57.
The fractionation of nuclear extract and immunopurification of BRG1, hbrm, and BAF60b complexes have been previously described (17, 26). The purified BAF complex from KB cells were separated on 8% SDS gel and transferred to polyvinylidene difluoride (PVDF) membrane. The band corresponding to BAF57 was excised and digested with trypsin. The peptides mixture were fractionated on a reverse-phase HPLC and subsequently sequenced by Edman degradation. The peptide sequences obtained were used to search the NR and dbEST database at the National Center for Biotechnology Information (NCBI) by using the blast program. The sequences were found to match multiple expressed sequence tag (EST) sequences from both human and mouse (access nos. T34541, T36278, D21493). PCR primers were designed based on the EST sequences and were used to amplify the EST DNA by using the cDNA of human Jurkat T cells as the template. The PCR product was then used as a probe to obtain the full-length cDNA as described (26). There is a in-frame stop codon upstream of the predicted initiator metheonine in both human and mouse cDNAs. The sequence alignments were performed by using bestfit, pileup, and pretty of the uwgcg program. Two rabbit polyclonal antibodies were raised against two different regions of BAF57: BAF57H (aa 63–153) and BAF57C (aa 316–411). These regions were fused to maltose-binding protein (New England Biolabs) and the recombinant fusion proteins were expressed and purified according the manufacturer's protocol and used as immunogen. BAF57 complex was immunopurified with BAF57C antibody by using conditions identical to those for BRG1 complex. The Western blotting conditions are also the same as BRG1 antibodies (17).
Establishment of Cell Lines That Stably Expresses Hemagglutinin (HA)-Tagged BAF57 and Purification of the Complex by Using Antibody Against HA.
The coding region of BAF57 was amplified by PCR to generate appropriate restriction ends and cloned into pBJ5-neo vector that was designed to have a HA epitope at 3′ end of the gene. The plasmid (10 μg) was transfected into Jurkat T cells (1 × 107 cells) by electroporation, and stable transformants were selected with G418 (1 mg/ml). The clones resistant to G418 were screened for expression of HA-tagged BAF57 by Western blot analysis with both 12CA5 mAb and BAF57 antibody. Four independent clones were obtained. Purification of HA-tagged BAF57 complex from three different clones yield identical set of polypeptides as visualized on silver-stained gels. The immunopurification with 12CA5 antibody was similar to that used for purification of HA-tagged TFIID (35). The antibody-protein A beads were prepared as described (17). The crude nuclear extract from the stable cell line (1.3 ml, 7 mg/ml protein concentration) was centrifuged at 100,000 × g to remove cell debris. The supernatant was applied directly to 100 μl of antibody beads and the mixture was rotated at 4°C for 5 h. The beads were then washed four times with 0.5 M buffer D (20 mM Hepes, pH 7.9/0.5 M KCl/0.25 mM EDTA/10% glycerol/1 mM DTT/0.1% Tween-20), once with buffer D lacking KCl, and once with 0.2 M buffer D. The complex was eluted off the beads by incubation at room temperature for 0.5 h with the HA epitope peptide (Anagen) at 1 mg/ml in 0.2 M buffer D.
Expression and Purification of Recombinant BAF57 in Escherichia coli.
BAF57 and its truncated version (containing N-terminal 163 amino acid residues) were expressed with a 6× histidine tag and purified with a Ni2+ column according to the manufacturer's instructions (Qiagen, Chatsworth, CA). Some contaminating E. coli proteins were removed by heat inactivation at 70°C for 5 min. The recombinant protein was over 95% pure as determined by SDS/PAGE (12%) and used in DNA binding studies. Further purification of the protein was performed by preparative SDS/PAGE. The protein was eluted from the gel and renatured. The renatured protein still retains binding activity to four-way junction (4WJ) DNA. The generation of K112I mutant was done by PCR-mediated mutagenesis. The mutant protein was similarly purified but without the step of preparative SDS/PAGE.
Gel-Shift and Mononucleosome-Disruption Assays.
The 4WJ DNA and its two duplex DNA "arms" were made according to Bianchi (36). The 20 μl reaction mixture for the gel-shift assay contains 0.1 ng p32-labeled probe, 10 mM Tris⋅HCl (pH 7.5), 100 mM KCl, 5 mM MgCl2, 5% glycerol, 0.1 mM DTT, 0.01% spermidine, 0.1 mg/ml BSA, 300 ng poly(dI-dC), and indicated amount of recombinant protein or complex in the figure legends. The reaction was performed at 4°C for 30 min, and the mixture was analyzed on a 4% native gel (0.5× TBE, the acrylamide to bisacrylamide ratio is 30:1 for recombinant proteins and 80:1 for the BAF57 complex). The electrophoresis was run at 4°C at 10 V/cm. The mononucleosome disruption assay with the 5S DNA as the template was done as described for the yeast complex (8).
RNase Protection Assay for BAF57 mRNA Levels.
The conditions for RNase protection assay is essentially the same as those previously described (37). The BAF57 probe was generated by first cutting pBluscript–mBAF57 cDNA plasmid with BamHI to remove the cDNA from 632 bp to 1,405 bp. The resulting construct was then linearized with BglII and then transcribed with T3 RNA polymerase. The protected fragment should be 220 nucleotides. The actin probe has been described (38).
Purification and Cloning of BAF57.
The BAF57 protein was purified by isolating the BAF complex from human KB cells by using conventional chromatography and immunoaffinity methods (17) (Fig. 1a, lane 2). Five peptide sequences were obtained from microsequencing (see Materials and Methods), which did not match any known genes in the NR databank maintained at NCBI. Based on the peptide sequences, a human cDNA clone was isolated and sequenced. A single ORF was identified containing all five peptide sequences obtained from the pure protein (Fig. 2a).
BAF57 Sequence Contains an HMG Domain and Kinesin-Like Coiled-Coil Region.
Examination of the predicted sequence of BAF57 reveals that it contains a single HMG domain between amino acids 66 and 133 (Fig. 2 b and c). Previous work has shown that the HMG domain serves as a DNA binding motif (39) for not only the abundant HMG chromosomal proteins, but also specific regulators of transcription and cell differentiation. Current evidence suggest that it plays an "architectural" role during formation of higher-order nucleoprotein complexes (40, 41). The HMG domain-containing proteins have been classified into two families: a sequence-nonspecific family that includes HMG chromosomal proteins, and a sequence-specific family that includes transcription factors LEF1 and SRY (reviewed in ref. 42). The HMG domain of BAF57 belongs to the nonspecific HMG family because amino acid residues corresponding to positions 66, 126, and 131 are all identical to members of this family and are distinct from those of the sequence-specific HMG family. However, proteins of the nonspecific HMG family usually have two or more HMG domains, whereas those of the specific family usually have a single HMG domain. In this later regard, BAF57 is more similar to the specific family. The BAF57 sequence also contains a region with two heptad repeats similar to the coiled-coil region of kinesin (Fig. 2d). The sequence similarity to kinesin extends beyond those residues necessary for the leucine zipper structure, suggesting a similar function. A region rich in acidic residues (net negative charge −28) was found near its C terminus (Fig. 2b), which is similar to the HMG1 protein that also contains a highly negatively charged C terminus.
BAF57 Homologues Are Present in Higher Eukaryotes but Not Yeast.
The mouse homologue of BAF57 was isolated by using the human cDNA as the probe. Comparison of the two sequences revealed that they are highly similar to each other (97% identical, 99% similar at amino acid level) and their HMG domains are identical. A partial Drosophila cDNA sequence has been found in dbEST databank which shows significant homology to human BAF57 (70% identity and 90% similarity within the HMG domain) (Fig. 2c). Preliminary data suggest that this cDNA encodes the homologous subunit of BAF57 within the Drosophila SWI/SNF-like complex (G. Daubresse, W.W., and M. Scott, unpublished data). We could not find any ORFs that have significant homology to BAF57 outside the HMG domain from the completed genome database for Sacchromyces cerevisiae. None of the yeast SWI/SNF subunits identified so far contains a discernible HMG domain. The remaining three uncharacterized subunits of the yeast SWI/SNF complex do not contain any HMG domains (B. Cairns, personal communication). Thus, BAF57 is present only in SWI/SNF-like complexes from higher eukaryotes but not yeast, underscoring the differences between these complexes.
BAF57 Is a Core Component Shared by Different Mammalian SWI/SNF-Like Complexes.
Two rabbit antibodies were made against BAF57 by using two different regions of the predicted ORF as immunogens (Materials and Methods). Both antibodies recognize the 57-kDa subunit in the BRG1 complex by Western blot analysis (Fig. 1b Left; data not shown), indicating that the isolated cDNA does encode BAF57. The antibodies also recognize the 57-kDa component of complexes purified with antibodies against hbrm (17) and BAF60b (26) (Fig. 1b Middle and Right; data not shown). Western blot analysis the polypeptides purified with the BAF57 antibody column revealed the presence of every known mammalian BAF subunit (Fig. 1b; data not shown). The pattern of these BAF57-associated polypeptides on a silver-stained SDS gel is remarkably similar to those associated with the BRG1 complex (Fig. 1a, compare lanes 1 and 2), indicating that BAF57 is a core subunit shared among different mammalian BAF complexes.
BAF57 mRNA was detected in all tissue examined at about equal levels (Fig. 3a). The BAF57 protein was also found in cell lines derived from several different tissues by Western blot analysis (Fig. 3b). Thus, BAF57 appears to be ubiquitously expressed, similar to other core subunits of the complex [BAF170, BAF155, and BAF47/hSNF5 (26)].
The Recombinant BAF57 HMG Domain and the Mammalian BAF Complex Bind 4WJ DNA.
A unique feature of the HMG domains is their high affinity for DNA with sharp bends such as 4WJ DNA and low affinity for duplex DNA (43) (Fig. 4a). We investigated the DNA binding properties of BAF57 by expressing it as a recombinant protein in E. coli. The full-length protein was expressed at very low levels and has many degradation and/or premature termination products. A truncated protein containing the entire HMG domain was found stably expressed at high levels and was purified to homogeneity (Fig. 4b). This protein shows strong binding activity to 4WJ DNA in gel-shift assays (Fig. 4c, and data not shown), which is believed to mimic the entry and exit points from the nucleosome (44). As a control, a point mutant was created by changing a conserved lysine residue within the HMG domain to isoleucine (K112I, Fig. 2c). The mutant protein was purified (Fig. 4b, lane 2), and showed at least 10-fold lower DNA binding activity compared with the wild type (Fig. 4c, lanes 6–9). An identical mutation has previously been described in the HMG domain of SRY that similarly decreased the DNA binding activity (45). When the duplex "arms" of the 4WJ DNA (depicted in Fig. 4a) were used as probes, the recombinant BAF57 proteins showed no detectable binding activity even at a concentration 10-fold higher than those necessary to bind 4WJ DNA (Fig. 4c, lanes 6–9). These DNA binding properties of BAF57 are similar to the HMG1 protein and the yeast SWI/SNF complex (34), but different from two other HMG domain-containing proteins, LEF1 and mTF1 (Fig. 4 c and d). In addition, BAF57 showed no detectable binding to 12 other duplex probes of different sequences and lengths (data not shown). Our attempts to define a consensus binding site for BAF57 by using several binding site selection methods were unsuccessful. Thus, BAF57 appears to be highly selective for 4WJ DNA as is the yeast SWI/SNF complex (34).
To develop a way of studying the DNA binding characteristics of complexes having mutant BAF57 proteins, we established a stable cell line expressing BAF57 tagged with the HA epitope and purified the BAF complex with a monoclonal antibody against HA (Fig. 5 a, lane 4, and b). The use of the mAb (12CA5) column allows the elution of the complex under near physiological salt conditions by using the HA peptide. The strategy has been used previously to purify the holo-TFIID complex (35). The HA-tagged exogenous BAF57 has a slower mobility on SDS gel compared with the endogenous BAF57, caused by the molecular weight increase by addition of the HA tag (Fig. 5b; also compare lanes 3 and 4 of Fig. 5a). This mobility difference provides a convenient way to distinguish the exogenous and endogenous proteins. The BAF complex purified with the anti-HA antibody contained only the HA-tagged BAF57, but not the endogenous BAF57. Because the level of the tagged protein in the cell is lower than that of the endogenous protein, the result suggests that there is only one BAF57 per complex (otherwise, the endogenous BAF57 will also be present in the complex purified with anti-HA antibody). This result contrasts to results with the SWI3-like subunits, BAF170 and BAF155, which are present in two copies per complex (26).
The BAF complex purified from the cells stably expressing the HA-tagged BAF57 was tested for its DNA binding properties by using the gel-shift assays and probes described above. This complex shows clear binding activity to 4WJ DNA (Fig. 6a), but no detectable activity to regular duplex DNA (Fig. 6b). The presence or absence of ATP in the reaction does not change the binding (Fig. 6a). Thus the mammalian BAF complex has similar DNA binding characteristics as the BAF57 HMG domain and the yeast SWI/SNF complex.
The BAF57 HMG Domain Is Not Essential for the DNA Binding Activity or the Nucleosome Disruption Activity of the BAF Complex.
We investigated the importance of the HMG domain in the context of the whole complex by using the above in vivo assembly method. We constructed two additional stable cell lines expressing HA-tagged BAF57 mutants: the same K112I mutation within the HMG domain that has been shown to decrease the binding affinity to 4WJ DNA by recombinant BAF57 by at least 10-fold (Fig. 4c), and a deletion mutant that removes the entire HMG domain (aa 62–131). Both mutant complexes were purified with antibody against HA and found to contain all other subunits (Fig. 5a, compare lanes 2 and 4; data not shown for the point mutant), indicating that the HMG domain is not involved in assembly of the complex (the N-terminal proline-rich region is important for assembly of the complex; unpublished data). Surprisingly, both mutant complexes have 4WJ DNA binding activity and ATP-dependent nucleosome disruption activity indistinguishable from the wild-type complex (Figs. 6a and Fig. 7; data not shown for the point mutant), suggesting the HMG domain is not essential or is redundant for these in vitro activities of the mammalian BAF complex.
We report the cloning of BAF57, a subunit of mammalian SWI/SNF-like complexes (referred to as BAF). At least three lines of evidence indicate that BAF57 is a component of all mammalian BAF complexes. First, BAF57 stably associates with all other BAF subunits even under highly stringent conditions, such as 1 M KCl or 0.2 M guanidine hydrochloride. Second, immunopurification with any existing BAF antibody copurifies BAF57. Third, complexes purified with the BAF57 antibody have similar subunit compositions as those purified by anti-BRG1, -hbrm, or -BAF60b antibodies (Fig. 1a) (17, 26). Because yeast do not have a homologue of BAF57, our data provide evidence of the structural differences between the mammalian BAF and yeast SWI/SNF-like complexes. Our preliminary data suggest that at least two other BAF subunits do not have yeast homologues and that several yeast SWI/SNF subunits do not have homologues in the BAF complex (unpublished data). Thus, although the BAF complex is similar to the yeast SWI/SNF and Rsc in several regards, it has significant differences in overall structure and possibly biological functions.
BAF57 Contains an HMG-Like DNA Binding Domain that Belongs to the Nonspecific HMG Family of Proteins.
HMG domains have been found in the abundant chromosomal proteins HMG1/2 as well as specific transcription factors such as UBF, LEF1, and SRY. They interact with minor grooves of DNA and can induce strong bending on association with their templates (40, 41, 46–48). Recent evidence suggests that they may not have biological activities on their own, but rather function as architectural factors during assembly of higher-order nucleoprotein complexes with other proteins (reviewed in ref. 42). The fact that the BAF complex contains an HMG domain-containing subunit implies that the complex may play an architectural role like other HMG domains. We demonstrated that the recombinant BAF57 HMG domain binds DNA similar to HMG proteins, with high affinity to 4WJ DNA and little affinity to linear DNA. To our knowledge, this is the first DNA binding subunit identified for the SWI/SNF-like complexes from any species. The yeast SWI/SNF complex has been shown to bind 4WJ DNA like HMG proteins (34), yet none of the subunits from the yeast complex has been shown to have DNA binding characteristics of HMG proteins.
The Mammalian BAF Complex Contains Additional DNA Binding Domains Other than BAF57.
We showed that the BAF complex has similar DNA binding characteristics as the recombinant HMG domain of BAF57 and the yeast SWI/SNF complex. They all have high affinity to 4WJ DNA and low affinity to linear DNA, indicating that the DNA binding properties of SWI/SNF-like complexes are conserved through evolution. However, the domain through which the BAF complex interacts with DNA or nucleosomes may not necessarily be BAF57, because the BAF complex containing HMG domain-deleted BAF57 still has intact DNA binding activity (Fig. 6) and the ATP-dependent nucleosome disruption activity (Fig. 7). Thus, the BAF complex must contain other DNA binding subunit(s). Without the HMG domain of BAF57, the complex is able to bind 4WJ DNA and disrupt nucleosomes through these other subunit(s).
What, then, is the role for the BAF57 HMG domain within the mammalian BAF complex? Because the yeast SWI/SNF complex has similar DNA binding properties as the BAF complex and it does not have a BAF57 homologue, the primary interactions of both complexes to DNA should be through subunit(s) other than BAF57. The interaction provided by BAF57 HMG domain to its target could be secondary or redundant for the mammalian complex. As a result, the mammalian complex may be able to interact with multiple nucleosomes and structured DNA through two or more DNA binding domains. The interaction through BAF57 may provide additional specificity during target recognition by the BAF complex. Because the intracellular concentrations of BAF in mammals or SWI/SNF in yeast relative to their DNA targets is not known, the in vitro binding studies reported here and for yeast (34) must be viewed with some skepticism.
Alternatively, BAF57 may not be involved in targeting at all but is used at some stage in the disassembly of chromatin from higher-order structure to a less compact structure. Because the DNA binding characteristics of the yeast and the mammalian SWI/SNF-like complexes have been studied on bare DNA, the role for the HMG domain of BAF57 may not be discernible with the available assays. Identification of the other DNA binding subunit(s) will help to elucidate the importance of BAF57 during DNA binding. Studies of the complex bearing mutant BAF57 proteins during development may be necessary to discern its biological functions.
We thank Dr. R. Tjian for his support and advice throughout this project. We also thank Drs. Hui Ge, R. Johnson, and S. Lippard for providing purified recombinant HMG1 protein; Dr. C. Pabo for LEF1; and Dr. D. Clayton for mTF1. We also want to thank Drs. Jacques Cote and Tom Hughes for advice and materials for nucleosome disruption assays. W.W. has been previously supported by fellowships from Damon Runyon–Walter Winchell Cancer Foundation (DRG-1182) and the Howard Hughes Medical Institute.
↵* Present address: Laboratory of Genetics, National Institute on Aging, National Institute of Health, Gerontology Research Center, 4940 Eastern Avenue, Baltimore, MD 21224.
↵‡ T.C., Y.X., S.Z., and A.K. made equal contributions to this work.
↵¶ To whom reprint requests should be addressed. e-mail: hf.grc{at}forsythe.stanford.edu.
This contribution is part of the special series of Inaugural Articles by members of the National Academy of Sciences elected on April 29, 1997.
Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. AF035262 and AF035263 for human and murine BAF57, respectively).
- BRG1-associated factor;
- high mobility group;
- four-way junction;
- expressed sequence tag;
- hemagglutinin;
- National Center for Biotechnology Information
- Accepted November 18, 1997.
- Copyright © 1998, The National Academy of Sciences
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text:
Mister MULLER Jean-Alain - Holiday accommodation 1
2 bedroom(s)
Phone CallEmailOfficial websiteBook
38Rue Charles de GaulleLocate
Résidence les Marronniers
Located in the center of Orbey. Apartment nice and fresh in summer, warm and cozy in winter. Large terrace. Two private car parks. possible to ski storage in the apartment. A cot can be made available. Starting point for hikes. 10 minutes from the Lac Blanc station. Near the most beautiful villages of Alsace.
Useful Information
- Possibility of Internet connection
- Internet Access
- Parking
- Yes
- Methods of payment
- ChequeCashTransfer
- Altitude (m)
- 500 m
- Distance from Lac Blanc 900
- 13,8 kms
- Distance from Lac Blanc 1200
- 11 kms
- SatNav Coordinates
- 7.1621956999999848.1267421


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How do I download my cookbook or recipes from MasterCook.com to MasterCook 14 on my computer?
If you are using version 15, you should never download. Use the Sync screen in the My MasterCook Manager program on the computer instead. Otherwise, you will corrupt your cookbook.
The default cookbook with the recipes you imported from the web will be in a cookbook called My Web Imports when you are on the page My Cookbooks.
Click on the Download link next to your cookbook. This downloads an .mz2 file to your computer. Open that MZ2 file with MasterCook 14 to import it into a cookbook.
Otherwise, to import selected recipes follow these instructions:
- Click on that cookbook
- After the page has loaded you can click on the list icon (second icon) next to VIEW: in the top right. This should switch the view mode to a list view.
- You can either click on each checkbox to the right of each recipe or select the top checkbox to select all recipes. Then click the download button.
- This will download the recipes to the default download location on your computer. (Name should include [RecipeExport.mz2]
- Once you located this cookbook double-click the downloaded file and MasterCook will open so you can import it.
Another option is to use the Download button located within the web import window as shown in this screenshot:


text:
Legislative panel unanimously backs call for special session on water rights
Thu., June 7, 2018
Lawmakers from both houses and both parties agreed Wednesday that Idaho needs a special legislative session this summer to deal with a long-simmering water rights fight in the Treasure Valley.
Only the governor can call lawmakers back to Boise for a special session; a joint interim committee voted unanimously to send Gov. Butch Otter a letter requesting that he do just that.
Otter, who was traveling in eastern Idaho, had no immediate comment on Wednesday.
At issue is the water in three sparkling reservoirs east of Boise — Anderson Ranch, Arrowrock and Lucky Peak — where Boise River water is held before being sent on downstream. That network "irrigates and provides the water for the fastest-growing area in the fastest-growing state in the nation," House Speaker Scott Bedke told the Legislature's interim natural resources committee. "That means competition for limited resources, including water."
One of the major functions of the reservoir system is flood control.
"This is a really significant issue for folks in the Treasure Valley," said Al Barker, attorney for the Boise Project Board of Control, which functions as the delivery entity for water headed down the river to 167,000 acres of subdivisions, farms, parks and more. "There are constraints over how much water the river channel can accommodate. So you can't just hold all the water back until you've got the reservoirs filled and then dump what you don't need, because you'd just flood out this entire valley. … You've got to release it as you go … to protect life and property in this valley."
The federal Bureau of Reclamation does that — it releases water from the reservoirs as the snow melts off the mountains, to make sure there's room for all the runoff.
But under Idaho law, holders of senior water rights in the reservoirs could see their water dumped early in the spring — long before they need it — because of flood-control needs. Those water users want guarantees that they'll get their water later in the season, when the reservoirs fill back up.
"How do you ensure both fill of the reservoir and flood control? Well, you provide a water right that allows and recognizes the fill of the reservoir," Barker said.
Water law in Idaho gives priority to those who have held their water rights the longest, under the prior appropriation, or "first in time, first in right" doctrine. So making any changes in the rules can cause huge ripple effects among various classes of water rights holders, from cities to farmers to the federal government.
Four years ago, when similar issues were raised in the Snake River Basin, a settlement was reached to allow for refill. That's been harder to achieve in the Treasure Valley, as multiple contested cases have wound their way through courts, administrative appeals, cross-appeals and more; a major set of appeals is currently set for oral arguments at the Idaho Supreme Court on June 20.
Bedke has been working with all sides to broker a settlement that would make further court fights unnecessary. After the committee's unanimous vote Wednesday, he said, "I would urge the parties to show good faith at this point, and make a motion to postpone the June 20 oral arguments."
Bedke said he determined that there were three concerns among the various parties in the Treasure Valley that were holding up a settlement of the refill issue. All were about future possibilities: Something that would move water from the Treasure Valley out of the basin; major aquifer recharge projects, which can use up large quantities of water; and new water storage projects, such as raising Arrowrock Dam or even building an additional dam. None of those are actually in the works, though the Arrowrock idea has been discussed.
Working with lawyers on all sides, Bedke said the parties determined that the first two concerns could be addressed under current law, through applications for decrees to the state's water court. The third, about new water storage projects, requires a change in state law.
So draft legislation was put together saying that applications for big new water-storage projects should have lower priority for water rights than existing rights for water in reservoirs.
"That makes it very clear that new storage projects and their filling is not going to come ahead of refilling of the old reservoir system," Bedke said. "It is that simple."
Sen. Michelle Stennett, D-Ketchum, the Senate minority leader, wondered how the change would impact other parts of the state, and questioned "what the urgency or the emergency is here" to justify the time and expense of calling a special session of the Legislature.
Barker said, "This piece of legislation is an essential part of the settlement. Without the legislation, without an indication we've got a chance of the legislation, the agreement could very well fracture, and we'd be back doing what we've been doing in the last decade."
Bedke said when he was asked to help mediate the dispute back in December, he went to all the lawyers on all sides. "I said, 'What are the probable outcomes if these contested cases proceed through the Supreme Court?' I got as many opinions as lawyers that I asked."
And when he asked about the time and costs involved, the estimates were that the issue would probably take another three years to flow through the court system, and cost the parties on all sides another $1.5 million — roughly equal to what they've spent so far.
House Majority Leader Mike Moyle, R-Star, said the issue affects roughly half the state's population, and impacts farmers, cities, fisheries and more.
"There's been a lot of blood, sweat and tears gone into this agreement, and for this agreement to go forward, this piece of legislation needs to pass," he said. "If you think this through, we probably need to have a special session to save the state money. By not having a special session, you keep this issue going out there."
Moyle proposed that the committee back the bill and request the governor to call a special session, but Sen. Maryanne Jordan, D-Boise, said she couldn't vote in favor of the bill "before I hear all the testimony."
Bedke said a special session — which could be as short as a single day, and would be limited to just this issue — would include full public hearings and debates in both houses.
When Moyle altered his motion to supporting the concept and recommending a special session, every member of the joint panel supported it.
Afterward, Stennett said, "In the interest of transparency, it's important that debate on this very complex issue is held in public view so that everyone has a chance to ask questions and voice any concerns they may have. This legislation could impact water rights around Idaho."
Idaho Water Resources Director Gary Spackman said late Wednesday that suggestions that his department has signed off on the plan "went a little beyond where I've agreed to be." While he's not opposing the legislation, he's also not supporting it at this point.
"I think there needs to be a full airing," he said.
The Idaho Attorney General's office had no comment.
Bedke said the legislation isn't the only way the dispute could be resolved — but it's the one that, after years of dispute, all sides are settling on. "The negotiations have led us here today," he said.
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What Is An FHA Loan? | 2019 Complete Guide | Bankrate.com – An FHA loan is a government-backed mortgage insured by the Federal Housing Administration, or FHA. Popular with first-time homebuyers, FHA home loans require lower minimum credit scores and down.