text:
Goldsmith Court, Nottingham
Out of the country?
Try a video call viewing
Goldsmith Court, Nottingham
Our Classic En-suite rooms are designed with comfort in mind, complete with single bed, en-suite bathroom, study desk, wardrobe and plenty of storage space. These rooms are located within flats varying from 3 to 8 bedrooms with a shared kitchen/dining area complete with a 4 ring ceramic hob, single oven, microwave, fridge-freezer and dining table.
Contact information
Tel: +44(0)1159474078
Goldsmith Court,
Chaucer Street,
Nottingham, NG1 5LG
Not found the information you were looking for about Goldsmith Court or our student accommodation in Nottingham? You might find the answer in our frequently asked questions.Visit our FAQs


text:
The Panthers of C.E. King high school are officially playing football on the same NFL home field as the 2017 Houston Texans. The ribbon cutting ceremony marked the one-year anniversary of Hurricane Harvey with the christening of their Hellas field at Crenshaw Stadium in North East Harris County.
Two-Time NFL Defensive Player of the Year J.J. Watt helped raise $37 Million for Harvey relief. The Texans played on Hellas Matrix® Turf with Helix Technology in 2017 at NRG Stadium. Now this same field has been moved to the Panther's home stadium as part of a donation program to Harris County schools in need. Hellas went to Harris County, owner of NRG Stadium, and recommended C.E. King to be the first recipient of the Texans' turf donation and the county agreed. For the next couple of years, Hellas has a contractual agreement to replace the Matrix Turf with Helix Technology at NRG Stadium following the Houston Livestock Show and Rodeo and donate it to a school in need in Harris County.
Days after Hurricane Harvey devastated Crenshaw Stadium with close to 50 inches of rain, Hellas crews repaired and sanitized the Panthers' field. C.E. King did not open until January of this year due to the damage from Harvey. A new C.E. King high school and stadium is set to open in 2020. "Sheldon Strong" is the motto of the school and the Panthers prove it daily.
C.E. King Athletic Director and Head Football Coach Derek Fitzhenry said, "Hellas really helped us. They moved us to the front of the list to sanitize our field and clean it." Fitzhenry added, "You just had sewer water on the field. That's going to be a big deal as far as cuts and abrasions. We had students and staff displaced from their homes but when it came time for the games, the parents and community responded and were there to support our players. Our kids had a successful year and due to Hellas, the seniors enjoyed their senior year playing all five home games at their home stadium."
Frank Petrini, COO of Hellas Construction said, "Those kids of C.E. King were just super excited today when they came off the field. The legacy of the Texans 2017 field out of NRG Stadium, just brings it to a whole new level." Hellas Construction, Inc. headquartered in Austin, TX, is one of the largest sports construction contractors in the United States. Specializing in the general construction of sports facilities and sports surfaces. Hellas champions innovative artificial turf manufacturing, base construction, field, track and tennis planning, installation and maintenance. hellasconstruction.com

subset: commoncrawl


text:
Many recent studies have shown that excitatory synapses can contain NMDA receptor responses in the absence of functional AMPA receptors and are therefore postsynaptically silent at resting membrane potentials. The activation of silent synapses via the rapid acquisition of AMPA receptor responses may be important in synaptic plasticity and neuronal development. Our recent immunocytochemical studies that used cultured hippocampal neurons have provided evidence for "morphological silent synapses" that physically contain NMDA receptors but no AMPA receptors. Here we show that the activation of NMDA receptors by spontaneous synaptic activity results in the rapid recruitment of AMPA receptors into these morphological silent synapses within minutes. In parallel, we find a significant increase in the frequency of AMPA receptor-mediated miniature EPSCs (mEPSCs). NMDA receptor activation also results in a mobilization of calcium/calmodulin (CaM) kinase II to synapses and an increase in the phosphorylation of surface AMPA receptors on the major CaM kinase II phosphorylation site. These results demonstrate that AMPA receptors can be modified and recruited rapidly to silent synapses via the activation of NMDA receptors by spontaneous synaptic activity.
- long-term potentiation
- long-term depression
- synaptic plasticity
- GluR1
- calcium/calmodulin-dependent protein kinase II
- mEPSC
In the CNS the majority of excitatory synaptic transmission occurs at glutamatergic synapses and is mediated via the AMPA-, NMDA-, and kainate-type glutamate receptors (Hollmann and Heinemann, 1994). Models of synaptic plasticity, such as long-term potentiation (LTP) and long-term depression (LTD) of glutamatergic synapses, are believed to underlie the cellular basis of learning and memory in the adult brain and the activity-dependent regulation of synapse formation in developing brain (Bliss and Collingridge, 1993; Malenka, 1994; Katz and Shatz, 1996). The molecular mechanism of such synaptic plasticity, however, is not known. Current evidence suggests that activity-dependent changes in synaptic strength, such as LTP and LTD, result at least in part from changes in AMPA receptor-mediated responses (Kauer et al., 1988; Muller et al., 1988;Davies et al., 1989; Liao et al., 1992). These changes may be attributable to altered levels of AMPA receptors in the postsynaptic membrane, altered receptor properties, or a combination of these two mechanisms.
It has been reported previously that AMPA receptors are phosphorylated and dephosphorylated during LTP and LTD, respectively, and that this may lead to changes in the ion channel conductance or open channel probability of AMPA receptors during these processes (Barria et al., 1997a; Benke et al., 1998; Lee et al., 1998, 2000; Derkach et al., 1999; Banke et al., 2000). Numerous recent studies suggest that the level of synaptic AMPA receptors also can be modulated by activity-dependent mechanisms (Kim and Huganir, 1999; Turrigiano, 2000). Both electrophysiological and morphological studies have indicated that a significant fraction of glutamatergic synapses contains NMDA receptors without detectable AMPA receptors (Isaac et al., 1995; Liao et al., 1995, 1999; Durand et al., 1996; Nusser et al., 1998; Petralia et al., 1999). Such synapses would be postsynaptically silent under normal resting potentials because of the voltage-dependent blockade of NMDA receptors by magnesium (Mayer et al., 1984; Nowak et al., 1984). Whole-cell recordings in brain slices demonstrate that such silent synapses can be activated via the acquisition of AMPA receptor-mediated responses after an LTP-inducing protocol that lasts only minutes (Isaac et al., 1995; Liao et al., 1995). The cellular mechanism underlying this rapid acquisition of AMPA receptor responses in silent synapses is not clear.
Several groups have reported that the chronic blockade of synaptic activity with CNQX or TTX for hours or days can cause a slow modulation in the amount of postsynaptic AMPA receptors in neuronal cultures (O'Brien et al., 1998; Turrigiano et al., 1998). It also has been reported that synaptic AMPA receptors in hippocampal cultures can be dispersed or internalized rapidly after a brief application of glutamate or an electrical LTD induction protocol (Lissin et al., 1998,1999; Carroll et al., 1999). Moreover, it has been reported recently that recombinant green fluorescent protein-tagged (GFP-tagged) GluR1 subunits can be mobilized rapidly to dendritic spines within minutes after a burst of high-frequency stimuli in cultured hippocampal slices (Shi et al., 1999; Hayashi et al., 2000). However, there is no direct morphological evidence that activity can regulate rapidly the recruitment of native AMPA receptors into silent synapses.
In this paper we have analyzed the rapid NMDA receptor- and activity-dependent modulation of synaptic AMPA receptors in both low-density hippocampal cultures and high-density cortical cultures. These cultured neurons have a large number of morphological silent synapses that contain clustered NMDA receptors but no detectable clustered AMPA receptors. Combining immunocytochemical and electrophysiological techniques, we demonstrate that these silent synapses rapidly become responsive to synaptically released glutamate via the recruitment of AMPA receptors after the activation of NMDA receptors.
Neuronal cultures. Low-density hippocampal cultures from 18-d-old embryonic rats were prepared as reported previously (Banker and Cowan, 1977). Neurons were plated onto 60 mm Petri dishes (Becton Dickinson, Bedford, MA) at a density of 120,000–200,000 cells per dish. Some dishes contained five coverslips per dish at a density of 1 × 106 cells per dish. In some cultures, d,l-aminophosphonovalerate (d,l-APV; 200 μm) was added to the culture medium to chronically block NMDA receptors at 5 d after plating.
High-density cortical cultures from 17- or 18-d-old embryonic rats were prepared as reported previously (Ghosh and Greenberg, 1995). Neurons were plated onto 60 mm Petri dishes (Becton Dickinson), with some dishes containing five coverslips per dish at a density of 6 × 106 cells per dish. Dishes that were used for biochemical experiments contained no coverslip. d,l-APV (200 μm) was added into the culture medium at 4 d after plating, and the same concentration of d,l-APV was maintained until usage. Thereafter, the cortical neurons were fed twice per week with feeding medium that had been incubated with glial cells overnight.
Induction protocol for synaptic targeting of AMPA receptors.High-density cortical neurons chronically treated with 200 μmd,l-APV were incubated in artificial CSF [aCSF; containing (in mm) 125 NaCl, 2.5 KCl, 26.2 NaHCO3, 1 NaH2PO4, 11 glucose, 2.5 CaCl2, and 1.25 MgCl2 at 37°C plus 5% CO2] in a tissue culture chamber for ∼15–20 min. Although sometimes an increase in the number of AMPA receptor clusters was observed after the withdrawal ofd,l-APV for 5 min (data not shown), a more consistent change often was induced by a treatment for ∼15 min. In control experiments, 200 μmd,l-APV was added to aCSF to block the NMDA receptors.
Withdrawal of d,l-APV alone in low-density hippocampal cultures was unable to induce a rapid mobilization of AMPA receptors. Most likely the low-density cultures lack the high levels of spontaneous activity that are necessary to provide both sufficient postsynaptic depolarization and released glutamate for the activation of NMDA receptors. In another protocol to induce synaptic targeting of AMPA receptors, we added glycine to aCSF to enhance the NMDA receptor channel opening and removed Mg2+ to allow for the activation of NMDA receptors at hyperpolarizing potentials. Cultured neurons were incubated in aCSF containing 2.5 mmCa2+, 100 μm glycine, and no added Mg2+ in a tissue culture chamber for ∼15–20 min. This protocol can induce a rapid mobilization of AMPA receptors in low-density hippocampal cultures growing in culture medium either with or without d,l-APV.
Immunocytochemistry. As described previously (Liao et al., 1999), cultured neurons were fixed successively with 4% paraformaldehyde, 4% sucrose in PBS (4°C, 20 min), and then with −20°C, 100% methanol (incubated at 4°C, ∼10–15 min); next the neurons were permeabilized with 0.2% Triton X-100 (4°C, ∼10–15 min). Coverslips with neurons were blocked in 10% donkey serum in PBS for ∼30–60 min and then incubated with primary antibodies dissolved in 10% donkey serum in PBS. All primary rabbit polyclonal antibodies were affinity-purified and conjugated either to Cy3 (red; Amersham Pharmacia Biotech, Arlington Heights, IL) or to Fluorescein-Ex (green; Molecular Probes, Eugene, OR). Mouse monoclonal primary antibodies were visualized with anti-mouse IgG antibodies linked to fluorescein isothiocyanate (FITC; green), rhodamine (red), or AMCA (blue; Jackson ImmunoResearch Laboratories, West Grove, PA).
Two rabbit polyclonal antibodies against the C terminus of the GluR1 subunit and the GluR 2/3 subunit, respectively, were used to detect AMPA receptor subunits. In most of the presented data the GluR1 and GluR2/3 antibodies were added together to detect AMPA receptors. A rabbit polyclonal antibody against the N terminus of the NMDA receptor 1 (NR1) subunit was used to detect this obligatory subunit of functional NMDA receptors. A mouse monoclonal antibody (MAB3119, Chemicon, Temecula, CA) was used to detect the α-isoform of calcium/calmodulin (CaM) kinase II (Erondu and Kennedy, 1985).
The visual field was moved blindly to a random site on a coverslip. In coverslips that were double-stained with antibodies against NR1 and AMPA receptor subunits, we first found and focused on a pyramidal neuron under the fluorescent channel that detected the NR1 staining. Thereafter, images of both NR1 subunits and AMPA receptor subunits were sampled by a digital camera (Princeton Instruments, Trenton, NJ) and analyzed with the MetaMorph Imaging System (Universal Imaging, West Chester, PA). In coverslips that were double-stained with synaptophysin and another antibody, the staining of synaptophysin was used for searching and focusing. Both NMDA and AMPA receptor clusters were significantly brighter than their surrounding areas. Almost all of them were marked as regions of interest by the computer when we set a threshold of fluorescent intensity that was slightly higher than the distant dendritic shafts but lower than cell body and proximal dendritic shafts. We included only those regions with an area >5 pixels and <500 pixels. The upper threshold limit for this selection was used to exclude the cell body and proximal dendritic shafts, which have much larger area than spines. We used a 100× objective to acquire our images (1 μm = 15 pixels; 1 μm2 = 225 pixels). Most of the clusters were <1 μm2 because the peripheral area of the selected cluster was not included. All regions of interest marked by the computer were compared with clusters that had been identified visually by the experimenter. Poor images without sharp NMDA receptor clusters and AMPA receptor clusters were discarded. Synaptophysin and CaM kinase II clusters are not sharp enough to be counted with this method. In the high-density neuronal cultures we were not able to trace dendrites and to normalize the number of receptor clusters to dendritic length. However, we have found that the density of synapses is similar between coverslips in sister cultures and is distributed randomly on the coverslip, allowing us to analyze the number of clusters per field and reliably compare the number of receptor clusters between culture dishes. In low-density hippocampal cultures the number of receptor clusters was normalized with the dendritic length after all of the dendritic branches in an image were traced and measured manually.
Immunoblotting and biotinylation of surface proteins.Cortical neurons were grown as described in the presence ofd,l-APV for 3–4 weeks and were subjected to the described induction protocol. After induction all of the dishes were cooled on ice and washed two times with ice-cold aCSF. Cultures then were incubated with aCSF containing 1 mg/ml sulfo-NHS-LC-biotin (Pierce Chemical, Rockford, IL) for 20 min on ice. Unreacted biotinylation reagent was quenched by three successive 5 min washes in ice-cold TBS (50 mm Tris pH 7.5, 150 mm NaCl). Cultures were lysed in modified RIPA buffer [1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid and (in mm) 10 NaPO4, 150 NaCl, 2 EDTA, 50 NaF, 10 sodium pyrophosphate, 10 iodoacetamide, 1 sodium orthovanadate, and 1 phenylmethylsulfonyl fluoride plus (in μg/ml) 2 aprotinin, 1 leupeptin, 2 antipain, and 10 benzamide] on ice and then harvested with a cell scraper. Crude lysates were cleared by centrifugation at 14,000 × g for 15 min at 4°C. A portion of the supernatant was added to SDS sample buffer for use as the total input for each sample. The remaining supernatant was diluted to a final volume of 1 ml in RIPA buffer. Then 100 μl of 50% NeutrAvidin agarose (Pierce Chemical) was added, and the samples were rotated for 3 hr at 4°C. Next the NeutrAvidin agarose was washed five times with RIPA buffer, and the bound proteins were eluted into SDS sample buffer by boiling for 15 min. Total protein and isolated biotinylated proteins were fractionated by SDS-PAGE and electroblotted onto Immobilon-P membrane (Millipore, Bedford, MA). Membranes were immunoblotted with affinity-purified antibodies against either GluR1 AMPA receptor subunits or the phosphorylation site-specific antibody against phosphorylated S831 of the GluR1 subunit (Roche et al., 1996; Mammen et al., 1997). All immunoblots were visualized by ECL development (Amersham Pharmacia Biotech) and quantified on a Storm Imaging System (Molecular Dynamics, Sunnyvale, CA). Surface expression was determined by calculating the ratio of the cell surface signal (signal from NeutrAvidin pull-down lane/percentage of total pull-down loaded on gel) and dividing this by the total input (signal in total input lane/percentage of total input loaded on gel) for each sample. For all experiments the input and pull-down samples were loaded on the same gels to minimize variability in different immunoblots. Loading controls were performed in all experiments to verify the linearity of quantified signals. Reported data represent the mean ± SE for the given number of dishes each for control and experimental groups harvested from four different dates of culture preparation.
Detection of phosphorylation of AMPA receptors. A phosphorylation site-specific antibody raised against a synthetic phosphopeptide (phosphorylated on serine 831) corresponding to the C terminus of GluR1 (GluR1–S831-P) has been well characterized previously (Roche et al., 1996; Mammen et al., 1997). This antibody was used for quantitative immunoblot analysis of total extract and cell surface proteins. Then the levels of GluR1–S831-P were compared with the respective levels of total GluR1 to determine the relative amount of the subunit population that was phosphorylated.
Electrophysiology. Whole-cell voltage-clamp recordings were established in high-density cortical neurons with a holding potential at −55 to −60 mV in aCSF (at 32°C, 95% O2/5% CO2) with 200 μmd,l-APV, 1 μm TTX, and 100 μm picrotoxin (a GABAA blocker). The internal solution in the patch electrode contained (in mm) 100 cesium gluconate, 40 HEPES, 2 Na-ATP, 0.3 GTP, 5 MgCl2, 5 glutathione, and 0.2 EGTA, pH-adjusted to 7.2 with CsOH. All recordings were filtered at 2 kHz. Series and input resistance was checked every 5 sec. Experiments with a series resistance >20 MΩ or with a change of series or input resistance >15% were discarded. There was no significant difference in the series resistances recorded at neurons with or without the withdrawal of d,l-APV (5.9 ± 0.8 vs 5.8 ± 0.6 MΩ; p > 0.05) and also no difference in input resistance (220.5 ± 75 vs 248.3 ± 25 MΩ;p > 0.05). The resting potential was slightly more negative than the holding potential (−55 to −60 mV) because the holding current was 0 to +20 pA at the moment when the plasma membrane was broken through. One recording sweep lasting 200 msec was sampled for every 1 sec. Detection criteria for miniature EPSCs (mEPSCs) included peak amplitudes >3 pA and a fast rise time and a slow decay time. A typical recording lasted ∼20–30 min. The starting point, the peak, and the ending point of a mEPSC were identified visually. The rise time was measured as the distance between the starting point and the peak. The decay time was measured as the distance between the ending point and the peak. To minimize variability in the density and maturation of recorded cortical neurons, we performed equal numbers of testing and control experiments in neurons from the same batch of cultures each day.
Data analysis. All data are reported as mean ± SE. Sample size n refers to the number of images processed in immunocytochemistry, the number of neurons in whole-cell recordings, and the number of dishes analyzed in biotinylation experiments. Groupt test was used to test the difference between the control and testing groups, and ANOVA was used to compare differences among several groups; *p < 0.05, **p < 0.01, and ***p < 0.001.
We have shown previously that low-density hippocampal cultures contain many "morphological silent synapses," synapses that contain NMDA receptors but no AMPA receptors, and that the proportion of these silent synapses can be increased by chronic treatment with the NMDA receptor antagonist d,l-APV (Liao et al., 1999). In these previous studies, however, we were unable to obtain rapid activity-dependent changes in AMPA receptor distribution, possibly because of a low level of spontaneous activity in these cultures. To circumvent this problem, we examined AMPA receptor targeting by using high-density cortical neuronal cultures that have much higher levels of spontaneous activity (Murphy et al., 1992). To maximize the number of silent synapses in the cortical cultures, we maintained them in NMDA receptor antagonists (Liao et al., 1999). Then the distribution of glutamate receptors in the cultured cortical neurons (23–26 DIV) was analyzed with the use of AMPA and NMDA receptor antibodies. The neurons were fixed, permeabilized, and double-labeled with a NMDA receptor NR1 subunit antibody directly coupled to FITC (green) and with GluR1 and GluR2/3 AMPA receptor subunit antibodies directly coupled to Cy3 (red). The cortical neurons contained a dense network of neurites with a high density of NMDA receptor clusters (Fig.1A,B). In contrast, the neurons had a diffuse distribution of AMPA receptors in the dendrites and contained few clusters of AMPA receptors (Fig.1A,B). The relatively few AMPA receptor clusters colocalized (>95%) with the NMDA receptor clusters (Fig.1A,B); however, most of the NMDA receptor clusters (55.7% ± 3%; n = 20) did not contain colocalized AMPA receptor clusters. The immunocytochemical labeling of both the diffuse and clustered receptors was blocked specifically by preincubation of the antibodies with the appropriate antigen (data not shown). To examine whether these receptor clusters were synaptic, we double-labeled the neurons with antibodies against NMDA or AMPA receptors and an antibody to the synaptic marker synaptophysin (Fig.1C–F). Similar to low-density hippocampal cultures (Liao et al., 1999), >90% of the receptor clusters colocalized with, or juxtaposed to, synaptophysin immunostaining, indicating that they are synaptic. These results demonstrate that, as with the low-density hippocampal cultures, many excitatory synapses in these high-density cortical cultures are "morphologically silent" and contain clustered NMDA receptors but no colocalized clustered AMPA receptors.
Despite the large number of "silent synapses" in these cultures, whole-cell recordings of the cortical neurons showed a robust network activity (data not shown) with bursts of high-frequency spontaneous synaptic activity (with frequency >100 Hz; duration ∼5–10 sec). We hypothesized that this activity might be able to trigger rapid NMDA receptor-dependent recruitment of functional AMPA receptors into silent synapses. To test this idea, we removed the d,l-APV from the chronically treated cortical neurons and then fixed the neurons 15–20 min later. Control neurons were maintained ind,l-APV. The neurons were fixed, permeabilized, and labeled with both GluR1 and GluR2/3 antibodies to analyze the AMPA receptor distribution. As discussed above, the cultures maintained in the presence of d,l-APV contained a large number of dendrites that had a diffuse distribution of AMPA receptors and contained few synaptic clusters of AMPA receptors (Fig.2A, top panels). This was in stark contrast to cultures that had thed,l-APV removed, in which the AMPA receptors had been redistributed rapidly within the dendrites, forming clusters (Fig.2A, bottom panels). In a quantitative analysis we found that the withdrawal of d,l-APV significantly increased the number of AMPA receptor clusters by 49% (from 622 ± 36 to 926 ± 26 per field; p < 0.001; n1 = 20, n2 = 20; Fig.2D). No significant change was observed in the number of NR1 receptor clusters (from 1340 ± 50 to 1261 ± 38 per field; p > 0.05; n1 = 20,n2 = 20; Fig. 2E). Similar to the control, we found that >95% of AMPA receptor clusters were colocalized with NR1 clusters after d,l-APV removal (Fig. 2A, bottom panels). We estimated the proportion of silent synapses by analyzing the percentage of NR1 clusters that were not colocalized with AMPA receptors. After the withdrawal of d,l-AP the proportion of silent synapses significantly decreased from 54.1 to 26.2% (p < 0.001; Fig. 2G). Withdrawal ofd,l-APV had no significant effect on the fluorescent intensity of either NMDA clusters or AMPA receptor clusters; however, we noticed a significant decrease in the apparent size of NR1 clusters after the withdrawal ofd,l-APV (from 68.9 to 57.5 pixels;p < 0.001; 1 μm = 15 pixels, 1 μm2 = 225 pixels; Fig. 2G) and a small increase in the apparent size of AMPA receptor clusters (from 71 to 75 pixels; p = 0.049; Fig.2F).
To test whether these new AMPA receptor clusters were truly synaptic, we double-labeled ∼26- to 30-d-old high-density cortical neurons with antibodies against synaptophysin and AMPA receptor subunits (GluR1 plus GluR2/3) after incubation with or without d,l-APV for ∼15–20 min. As shown in Figure 2B, almost all of the AMPA receptor clusters were colocalized or adjacent to a synaptophysin cluster after the withdrawal ofd,l-APV. In another set of experiments the cultures were double-stained with an anti-synaptophysin and an anti-NR1 antibody after incubation with or withoutd,l-APV. Similar to AMPA receptors, >95% of the NR1 clusters were colocalized or juxtaposed to synaptophysin staining after the withdrawal of d,l-APV (Fig.2C). To examine whether the synaptic recruitment of AMPA receptors is attributable to the activation of NMDA receptors by the observed high-frequency spontaneous synaptic responses, we used TTX to block sodium channel-mediated action potentials. In the presence of TTX the withdrawal of d,l-APV had no significant effect on the number of AMPA receptor clusters (from 607 ± 29 to 591 ± 32 per field; p > 0.05; n1= 20, n2 = 20; Fig. 2D) or NMDA receptor clusters (from 1310 ± 45 to 1340 ± 48 per field;p > 0.05; n1 = 20, n2= 20; Fig. 2E) and the proportion of silent synapses (from 53.6 to 55.8%; p > 0.05; Fig. 2G). To test further whether the mobilization of AMPA receptors was dependent on synaptic activity, we removed Ca2+ from the aCSF bath solution to block the release of presynaptic vesicles. In aCSF with 0 mm Ca2+ the withdrawal of d,l-APV did not affect the number of AMPA receptor clusters significantly (from 611 ± 31 to 565 ± 25 per field; p > 0.05; n1= 20, n2 = 20; Fig. 2D) or NMDA receptor clusters (from 1402 ± 44 to 1361 ± 36 per field;p > 0.05; n1 = 20, n2= 20; Fig. 2E) or the number of silent synapses (from 56.6 to 58.4%; p > 0.05; Fig. 2G). These results indicate that activation of NMDA receptors can induce a rapid mobilization of AMPA receptors into morphological silent synapses that previously contained only NMDA receptors but no AMPA receptors.
We next wanted to determine whether the new synaptic AMPA receptor clusters were present on the cell surface. Using the same induction protocol, we labeled the surface GluR1 receptors on live neurons with an antibody against the N terminus of GluR1. Withdrawal ofd,l-APV significantly increased the number of surface GluR1 clusters (from 672 ± 45 to 976 ± 37 per field;p < 0.001; n1 = 20, n2= 20; Fig. 3A,B) but had no significant effect on the number of NR1 clusters (from 1330 ± 43 to 1328 ± 38 per field; p > 0.05;n1 = 20, n2 = 20; Fig.3A,B). The proportion of morphological silent synapses significantly decreased from 50.2 to 26% (p < 0.001; Fig. 3B).
LTP induction results in the long-lasting increases in AMPA receptor responses. To examine whether the synaptic recruitment of AMPA receptors in our system is a long-term effect, we removedd,l-APV from the neurons for 15 min and then placed them back into culture medium containing d,l-APV for 2 hr. Under these conditions we observed a persistent increase in the number of AMPA receptor clusters (from 434 ± 18 to 685 ± 19 per field; p < 0.001; n1 = 20,n2 = 20; Fig.4A,B) and a persistent decrease in the amount of silent synapses (from 65 to 46%;p < 0.001; Fig. 4A,B).
To rule out that the mobilization of AMPA receptors was induced directly by action potentials via NMDA receptor-independent pathways, we added TTX to the aCSF bath solution to block action potentials and then removed d,l-APV and Mg2+in the presence of exogenous glycine to allow for the activation of NMDA receptors by the spontaneous release of glutamate. Although not as dramatic, under this condition the withdrawal of d,l-APV also significantly increased the number of AMPA receptor clusters (from 468 ± 26 to 592 ± 15 per field; p < 0.001;n1 = 20, n2 = 20; Fig.5A,B), had no effect on the number of NR1 clusters (from 1167 ± 36 to 1116 ± 29 per field; p > 0.05; n1 = 20,n2 = 20; Fig. 5A,B), and decreased the number of silent synapses significantly from 59.8 to 46.7% (p < 0.001; Fig. 5B).
Interestingly, a similar protocol also could be used to recruit rapidly the AMPA receptors in low-density hippocampal cultures despite the low intrinsic activity in these cultures. As with the TTX-treated cortical cultures, the activation of NMDA receptors in the low-density hippocampal cultures by d,l-APV and Mg2+ removal in the presence of exogenous glycine increased the number of AMPA receptor clusters (from 29.7 ± 3.3 to 88.8 ± 8.0/100 μm of dendrite; p < 0.001; n1 = 20, n2 = 20; Fig.6A,B) and reduced that number of silent synapses at hippocampal neurons from 73.8 to 23% (Fig. 6C). After this paradigm the silent synapses almost disappeared in one-half of the analyzed neurons, in which almost all of the AMPA clusters were colocalized with NR1 clusters (>90%; Fig.6A, bottom two panels). The recruitment of AMPA receptor to synapses also could be observed in the absence of chronic treatment of the neurons with d,l-APV. Although the initial proportion of silent synapses in cultures grown in normal medium was lower than the d,l-APV-treated cultures, the removal of Mg2+ in the presence of exogenous glycine increased the number of AMPA receptor clusters from 55.4 ± 4.8 to 98.6 ± 7.3/100 μm of dendrite (p < 0.001; n1 = 20,n2 = 20; Fig. 6E) and decreased the proportion of silent synapses (from 53.4 to 18.2%; Fig.6F).
If the AMPA receptors delivered into silent synapses were functional, we would expect to see a parallel increase in mEPSC responses after the withdrawal of d,l-APV. We induced synaptic targeting of AMPA receptors by incubating cortical neurons with aCSF in the absence of d,l-APV for 15–20 min. Thereafter, mEPSC responses mediated via AMPA receptors were recorded in aCSF [2.5 mmCa2+and (in μm) 200d,l-APV, 1 TTX, 100 picrotoxin at 32°C] with whole-cell voltage clamp. Withdrawal of d,l-APV significantly increased the frequency of mEPSCs (from 0.87 ± 0.15 to 2.1 ± 0.28 responses/200 msec; p < 0.01;n1 = 12, n2 = 12; Fig.7A,B), although it had a negligible effect on the mean peak amplitude (from 12.3 ± 0.6 to 12.1 ± 0.7 pA; p > 0.05; Fig. 7C), rise time (from 2.4 ± 0.1 to 2.5 ± 0.1 msec;p > 0.05), and decay time (from 8.9 ± 0.5 to 8.9 ± 0.4 msec; p > 0.05; Fig. 7C). In the presence of TTX the withdrawal of d,l-APV no longer had any obvious effect on the frequency of mEPSCs (from 1.1 ± 0.25 to 0.93 ± 0.11 responses/200 msec;p > 0.05; n1 = 6, n2 = 6; Fig. 7B). In aCSF with 0 mmCa2+ the withdrawal ofd,l-APV also had no significant effect on the frequency of mEPSCs (from 0.87 ± 0.14 to 0.84 ± 0.12 responses/200 msec; p > 0.05; n1 = 9,n2 = 9; Fig. 7B). These results suggest that silent synapses can acquire AMPA receptor-mediated responses after the activation of NMDA receptors by the spontaneous synaptic activity. Although we did not detect a significant change in the mean amplitude of mEPSCs after d,l-APV removal, the distribution of the mEPSC amplitudes shifted and contained higher percentages of large (>30 pA) and small (<10 pA) mEPSC responses (Fig.7D).
Many previous reports have indicated that CaM-dependent kinase II plays a critical role in the induction of LTP (Silva et al., 1992; Giese et al., 1998; Soderling and Derkach, 2000). Recent studies also have shown that CaM kinase II is recruited rapidly to synapses after LTP induction in hippocampal slices (Ouyang et al., 1997, 1999; Shen and Meyer, 1999), where it phosphorylates the AMPA receptor GluR1 subunit of the AMPA receptor (Barria et al., 1997b; Lee et al., 2000). To examine whether similar processes occurred in the high-density cortical cultures, we analyzed the distribution of CaM kinase II and the phosphorylation of GluR1 before and after d,l-APV removal. Using immunocytochemical techniques with CaM kinase II antibodies, we found that, similar to LTP in hippocampal slices, the activation of NMDA receptors in cortical cultures induced a rapid mobilization of CaM kinase II to synapses (Fig.8A,B). In contrast, no corresponding increase was observed in the control cultures maintained in d,l-APV (Fig. 8A,B). Moreover, using Western blot techniques with a phosphorylation site-specific antibody against the major CaM kinase II site on GluR1 (serine 831), we found that phosphorylation of serine 831 was significantly increased in total cell extracts (0.227 ± 0.010 to 0.418 ± 0.034; p < 0.005; n1 = 6, n2 = 6; Fig. 8C,D). Interestingly, this increased phosphorylation occurred preferentially on surface receptors isolated with the use of surface biotinylation techniques (from 0.132 ± 0.008 to 0.317 ± 0.028; p < 0.001;n1 = 6, n2 = 6; Fig.8E,F). Subtraction of the GluR1–S831-phospho surface signal from the GluR1 total reveals that, although phosphorylated at a basal level, there is only a small, but nonsignificant, increase in GluR1–S831 phosphorylation of intracellular AMPA receptors (from 0.336 ± 0.137 to 0.421 ± 0.174; p > 0.05; n1 = 6,n2 = 6). Phosphorylation of GluR1 serine 845 was not detectable in these experiments (data not shown). These results demonstrate that the activation of NMDA receptors by spontaneous activity in cultured cortical neurons can induce coincident and rapid translocation of AMPA receptors and CaM kinase II to synapses and increased CaM kinase II phosphorylation of the GluR1 AMPA receptor subunit.
Changes in AMPA receptor-mediated responses have been proposed to be important to various forms of synaptic plasticity, including LTP and LTD (Raymond et al., 1993; Kim and Huganir, 1999; Turrigiano, 2000). Electrophysiological studies have reported that a significant proportion of synapses in the CNS contains NMDA receptor responses, but no AMPA receptor responses (Isaac et al., 1995; Liao et al., 1995;Durand et al., 1996). Such synapses would be functionally silent at normal resting membrane potentials because of the voltage-dependent blockade of NMDA receptors by Mg2+ (Mayer et al., 1984; Nowak et al., 1984). These silent synapses progressively become active via the acquisition of AMPA receptor-mediated responses during neuronal development and can be activated rapidly during LTP (Isaac et al., 1995; Liao et al., 1995; Durand et al., 1996; Wu et al., 1996). Recent evidence has suggested that the activation of AMPA receptor responses is attributable to rapid changes in the level of synaptic AMPA receptors (Shi et al., 1999; Hayashi et al., 2000). In these previous studies it was shown that recombinant GFP-tagged GluR1 subunits expressed in organotypic hippocampal slices can be recruited to synapses by LTP-inducing stimuli (Shi et al., 1999; Hayashi et al., 2000). In this current study we combined immunocytochemical, biochemical, and electrophysiological techniques to study the NMDA receptor-dependent synaptic delivery of native AMPA receptor subunits in both high-density cortical and low-density hippocampal neuronal cultures. We predicted that high levels of spontaneous synaptic activity in cortical neuronal cultures might be able to activate NMDA receptors in a way similar to tetanus stimulation, a method often used to induce LTP. To increase the number of silent synapses in our cultures, we chronically treated the cultures with d,l-APV, starting at a very early stage of in vitro development. Similar to our previous studies in low-density hippocampal cultures,d,l-APV treatment produced a large proportion of silent synapses in these high-density cortical cultures (Rao and Craig, 1997; Liao et al., 1999). After the withdrawal ofd,l-APV the vast majority of dendrites that previously contained diffusely distributed AMPA receptors rapidly acquired clustered AMPA receptors. Withdrawal ofd,l-APV also induced a parallel increase in the frequency of mEPSC responses. A blockade of spontaneous activity by TTX or 0 mm Ca2+prevented this synaptic delivery of AMPA receptors. These results indicate that the high levels of spontaneous synaptic activity in high-density cortical cultures can induce a rapid NMDA receptor-dependent mobilization of AMPA receptors into silent synapses. In addition, we found that we could circumvent the low level of spontaneous activity in low-density hippocampal cultures and rapidly recruit AMPA receptors to synapses by activating NMDA receptors via the removal of d,l-APV and Mg2+ in the presence of glycine. Our results are similar to a recent study published after the completion of this work, showing NMDA receptor-dependent synaptic insertion of AMPA receptors in cultured dissociated hippocampal neurons (Lu et al., 2001).
Interestingly, the redistribution of AMPA receptors in these cultures is remarkably similar to the recently reported change of GFP-tagged GluR1 subunits induced by a tetanus stimulation in organotypic hippocampal slices (Shi et al., 1999). This similarity suggests that the redistribution of AMPA receptors in these two systems may be induced by similar cellular processes. In this study we further demonstrated that these newly acquired AMPA receptor clusters were located in synapses that were labeled by antibodies against NMDA receptor NR1 subunits and synaptophysin. This indicates that the previously silent synapses in these cultures were activated by the recruitment of native AMPA receptors. These data provide additional direct evidence that the activation of silent synapses may mediate, in part, the long-term potential of synaptic transmission.
Previous studies have demonstrated that the GluR1 subunit is a substrate for CaM kinase II, PKC, and PKA (Roche et al., 1996; Barria et al., 1997b; Mammen et al., 1997) and that phosphorylation of the GluR1 subunit can increase AMPA receptor ion channel function (McGlade-McCulloh et al., 1993; Roche et al., 1996; Barria et al., 1997a,b; Benke et al., 1998; Derkach et al., 1999). Recent studies in hippocampal slices have suggested that the modulation of AMPA receptor ion channel properties by protein phosphorylation may be important in LTP and LTD (Soderling and Derkach, 2000). It has been reported that LTP induction in slice preparations or glutamate treatment of neuronal cultures induces the rapid recruitment of CaM kinase II to excitatory synapses (Ouyang et al., 1997, 1999). Moreover, LTP induction increases CaM kinase II phosphorylation of serine 831 on the GluR1 subunit (Barria et al., 1997a; Banke et al., 2000; Lee et al., 2000). As seen in these LTP studies in hippocampal slices, we also observed a rapid synaptic targeting of CaM kinase II and an increase in GluR1–S831 phosphorylation. This phosphorylation and its effect on channel kinetics may account in part for the observed changes in the distribution of mEPSC amplitudes, but it is not clear whether the synaptic delivery of AMPA receptors and the changes in mEPSC frequency require the phosphorylation of serine 831. It is certainly possible that phosphorylation of this site regulates synaptic trafficking of the AMPA receptor in addition to regulating channel function. However, the regulation of synaptic recruitment of AMPA receptors may be via a distinct mechanism, which occurs in concert with and complements the modification of channel properties. The molecular and cellular mechanisms that regulate the mobilization of AMPA receptors into postsynaptic silent synapses are still not clear. Studies in several laboratories have suggested that proteins that interact with AMPA receptor subunits, such as the PDZ-containing proteins GRIP1/2/ABP, PICK1, and SAP97 as well as membrane fusion factor NSF, may regulate the function of AMPA receptors (Dong et al., 1997, 1999; Nishimune et al., 1998; Osten et al., 1998; Song et al., 1998; Li et al., 1999; Noel et al., 1999; Hayashi et al., 2000). Future studies will be needed to elucidate the role of these proteins in LTP and the mechanism for the coupling of CaM kinase II activation to the regulation of AMPA receptor distribution.
This work was supported by the Howard Hughes Medical Institute and the National Institutes of Health. We thank Doreen Bury for assistance in preparing this manuscript.
Correspondence should be addressed to Dr. Richard L. Huganir, Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205. E-mail: rhuganir{at}jhmi.edu.


text:
Will Entourage get a Hollywood ending?
TV SHOWS, like overindulged movie stars, must eventually grow up and that is what Entourage has grappled with in recent years. In the early seasons, as Vincent Chase (Adrian Grenier) moved effortlessly up the Hollywood ladder, taking childhood friends E, Turtle and Drama with him, it played out as a boy's own fantasy about the Hollywood high life and the sanctity of male friendship.
There were some gentle digs at the venality of the film industry but it was predominantly a world of soft drugs, easy sex and few consequences.
Then came the growing pains, as Vince's entourage began to develop their own careers and the show took a darker turn. This culminated last season when Vince hooked up with porn star Sasha Grey, developed a coke habit and self-destructed.
Our last look at Vince at the end of last season was of a fading superstar at his lowest ebb: bruised, bloody and busted with a bag of drugs.
This recognition of Hollywood's darker side might have given the show a veneer of realism but it detracted from the cartoonish sense of fun.
Read more at brisbanetimes.com.au


text:
Boutique escape on the Cornish coast
Just on the edge of the busy little town of Marazion, this boutique hotel offers contemporary indulgence and an exciting dining experience which is inspired by nature.
Modern this hotel may be, there's no doubt that you're deep in the heart of Cornwall. From its hillside setting the views out across St Mount's Bay are outstanding, while the menus created by head chef Ross Sloan showcase some of west Cornwall's best produce alongside lesser known native ingredients like foraged seaweeds, alexanders and sea asters.
Bag a table on the outdoor terrace at lunch and feast on freshly crafted sandwiches, mussels mariniere and an amazing view of St Michael's Mount. It's also a fine spot for a pre-dinner cocktail or glass of wine, perhaps with a tempting bar snack like the Asian-style salt and pepper squid.
If the weather takes a turn you can always move inside to the sleek restaurant with its cosy banquette seating, polished wooden floor and modern wood burner. Ross' inspired a la carte menu includes dishes such as hake, mussel fritters, celeriac, samphire and dulse, or mallard with beets, jerusalem artichokes, smoked potato and redcurrant.
This is a peaceful spot at any time of the year and, in true Cornish style, it has a well-kept, landscaped garden. There are also nineteen gorgeous bedrooms, along with a treatment room for the ultimate in coastal relaxation.
Insider's tip
Take a short walk into the market town of Marazion to explore its art galleries and indie shops before heading across the ancient causeway to St Michael's Mount.
Turnpike Road, Marazion, Cornwall, TR17 0DQ
01736 719937
Chef: Ross Sloan
Owner: James and Mary St Levan
Bedrooms: 19
Room rate from: £100
Restaurant opening hours
Monday to Saturday
Lunch: 12pm – 2pm
Dinner: 6pm – 9pm
Lunch: 12pm – 2.30pm
Dinner: 6pm – 9pm
- Kids welcome
- Dogs welcome
- Wifi
- Parking available
- Private dining available
- Gluten free options
- Exclusive use
- Online booking
Sample menu
Salt and pepper squid, radish, chilli, coriander, lemongrass, kaffir lime, £7.50
Pheasant, slow roasted heritage carrots, mushrooms, Polgoon cider, £17.50
Citrus baked cheese, curd, jelly, raspberry, sorbet, pistachios, caramelised milk powder, £8


text:
Brandeis is working hard to take what we're learning this fall semester and apply it to Spring 2021. The semester will begin on Feb. 1 and will employ a similar mix of remote and in-person learning, with enormous flexibility for students to decide what works for them. Read our announcement about the spring academic calendar.
Brandeis will provide an on-campus residential experience for undergraduate students this fall, with classes held in person, remotely or through a combination of both methods. Our graduate schools will offer their classes in a hybrid format. Learn more about our plans for the fall 2020 semester.
Brandeis students will have an experience that incorporates quality teaching and scholarship, comprehensive academic support services and community building.
Adapting to COVID-19 requires that we be deliberative, creative and agile. Faculty are participating in the Hybrid Teaching Institute. Student Affairs staff are developing new ways to engage students outside the classroom. And Brandeis alumni are planning to support students and graduates with mentoring, networking and even jobs.
The safety and well-being of our students, faculty, staff and the community we interact with is foremost in our minds. We are closely following guidance and protocols from the Massachusetts Department of Public Health and the federal government.
As a university founded on principles of inclusion and openness, we are committed to providing an equitable and accessible experience this fall for all of our students, faculty and staff.
Campus Updates
Read all coronavirus/COVID-19-related messages to the community.


text:
How to Navigate Harassment Claims in the "MeToo" Environment
April 2018
Workplace harassment claims have been on the rise as more employees come forward with allegations of inappropriate and potentially unlawful behavior at various levels of their organizations. Managing a company's response to such claims often requires navigating complex business and legal issues. In this seminar, we will cover:
- Examples of challenging harassment incidents that may arise in the workplace;
- Best practices for investigating and resolving such incidents; and
- Steps for handling employee complaints while effectively managing business and legal risks.
In addition, our "Quick Takes" will cover recent employment and benefits developments, including:
- Federal tax law restrictions on the deductibility of settlement payments in sexual harassment cases;
- New York City laws that expand the use of sick time and permit employee requests for temporary schedule changes; and
- U.S. Department of Labor's guidance on internship programs.
Gregg A. Gilman, Partner/Co-Chair, Labor & Employment
Shira Franco, Partner, Labor & Employment
Jessica Golden Cortes, Partner, Labor & Employment
David Fisher, Counsel, Labor & Employment
Sharon Cohen, Associate, Labor & Employment
Judith Kong, Associate, Labor & Employment
Gabrielle White, Associate, Benefits & Compensation
Registration and Breakfast: 8:30 - 9:00 a.m.
Seminar: 9:00 - 10:30 a.m.
Davis & Gilbert LLP
1740 Broadway, 19th Floor
(between 55th and 56th Streets)
New York City
Cost: Free Legal and NY HR CLE Credits: 1.5
Target Audience: Senior Management, In-House Counsel and Human Resource Professionals
RSVP: April 11, 2018 to Amanda Paredes
email@example.com / 646.673.8318


text:
I think we can all universally agree that times have been strange lately. I don't know how you have been passing the quarantine, but less time out and exploring the world meant more time at home for my sister and I. Due to this, we decided to finally tackle a goal of ours, and that … Continue reading Sourdough Starter Guide & Video
Tag: ladolcelisarecipe
Pavlova | Strawberries & Cream Meringue
This dessert reminds me of something my mother would make us when we were young. Her meringue style dessert had the addition of ground almonds I believe. It was one of my favourite desserts she ever made, the crunch of that meringue paired with the creamy cold whipped cream, and burst of sweet berries; it … Continue reading Pavlova | Strawberries & Cream Meringue
Carrot Cake Loaf | Cream Cheese Frosting
With a long quarantine upon us (at least in Canada), and much more time on our hands to cook and bake, I have been experimenting in the kitchen lately. I think we can all agree that leaving our house to grocery shop has actually become quite scary. With that being said, I wanted to bake … Continue reading Carrot Cake Loaf | Cream Cheese Frosting
Easy No-Yeast Bread Recipe
Hi everyone, I hope we are all staying home and staying safe during this difficult time. I for one have been cooking and baking a lot more than usual, which is the silver lining in this. Since there is difficulty in accessing certain ingredients, I remembered this great yeast-free bread recipe I would make all … Continue reading Easy No-Yeast Bread Recipe
Panna Cotta with Berry Sauce
One of the easiest and most delicious desserts you can make any time of the year, but especially now with Valentine's Day right around the corner is Panna Cotta. I have always loved this dessert, and once I made it, I could not believe how simple it was. The best part is, it is a no bake … Continue reading Panna Cotta with Berry Sauce
The Best Banana Bread Recipe
I have been perfecting my simple, soft and fluffy, chocolate chip Banana Bread recipe, with an easy and addictive sugary topping for quite some time. Since I was little, I would look forward to the bananas going brown in my house as an excuse to bake this banana bread. I really tried to develop a … Continue reading The Best Banana Bread Recipe
Key Lime Pie | Delicious & Easy Dessert
I have been making this dessert for years, and every time I bring it over to an event I always get asked for the recipe. People are always so impressed by this pie, that they don't believe me when I tell them how easy it is to make! I finally found Key Lime's at my … Continue reading Key Lime Pie | Delicious & Easy Dessert
Spinach Dip | Cold Spinach Dip Recipe
Hi and welcome back to another savoury recipe! This one is a bit different from the usual recipes I post because it's essentially an appetizer. This is also not really my recipe, but a recipe given to me by my sister's boyfriend's mother LUCY (thanks so much for letting me share this recipe Lucyna)! I … Continue reading Spinach Dip | Cold Spinach Dip Recipe
Italian Easter Pie | Ricotta Crostata
I am so excited to share this recipe with you all, and just on time for Easter too! This is a recipe I love to make (and eat) around this time of year, because it is perfect for Easter. Similar to an Easter Pastiera, this pie (or really tart) tastes like a cannoli pie - … Continue reading Italian Easter Pie | Ricotta Crostata
Zeppole Recipe | Baked AND Fried Zeppole + Pastry Cream
I don't know at what point this blog turned into a recipe blog, but I'm here for it! Today's post is no different because here is another recipe that I am sharing with you all, and it is DELICIOUS! I am very excited to show you how to make Zeppole di San Giuseppe (or just … Continue reading Zeppole Recipe | Baked AND Fried Zeppole + Pastry Cream


text:
The Best Credit Card for YOU: Part 1
There are many types of credit cards, and finding the best one for you depends completely on your lifestyle. So to give the best options for everyone's lifestyle, we're starting a series of posts to give advice on choosing the best credit card that fits you. Now credit cards can certainly be ranked by a vague list of all the offers presented, but the relative … Continue reading The Best Credit Card for YOU: Part 1


text:
RESIDENTS living in a Graiguecullen housing estate are allegedly being terrorised by 'neighbours from hell', with reports of children and parents being assaulted, adults fighting in public with machetes and stones being fired at motorists by slingshots.
The people of Heather Hill Square say they "are sick of it".
"Our children don't play on the street any more because they're afraid of getting hurt. Before this, the kids always played outside, but this whole summer, the children wouldn't play outside," one resident told reporters. She did not want to be named.
five-year-old girl "body-slammed into a car"
There were allegations of a five-year-old girl being "body-slammed into a car" by an older boy aged 12.
Another resident got hit with a stone in the face from a slingshot because he refused to buy alcohol for some teenagers, and in another incident residents were horrified to see two men fighting each other with machetes on the public green area.


text:
The new Purple Class children have settled well into the routine of the Turney School day. The children are now learning to work independently when we have our daily table work challenges.
The weekly trips to Brockwell Park have been continuing. We have been exploring the many different activities that take place in the park.
The pupils are also visiting the local shops each Friday. We take a prepared shopping list ahead of our food tech lessons.
We also look forward each week to our visit to the Turney School music studio with Miss G. Each class member is given a free choice of which instruments they would like to play. This then allows the staff to target any help the pupils might need in playing their instruments.
Different targets are set for each class member. Some of the children are working on how to manipulate the keyboards to create a sound. Co-ordination on the drums is another target that has been set. The ukulele players have been given help in how to hold the instrument correctly.


text:
It is a funeral, of sorts. Unworthy of landmark status and in the way of a development that will create twin seven-story buildings with more than 200 units of market rate housing along with more than 16,000 square feet of new commercial space on Broadway, Capitol Hill's Bonney Watson funeral home is being torn down this week.
The demolition of the 1961-constructed modern-style building the landmarks board called underwhelming, boxy, and, depressing is underway after the mortuary business active in Seattle since 1868 departed Capitol Hill for good last year.
In 2013, CHS reported on Bonney Watson's place on Broadway and in Capitol Hill history. 30 years ago, the busy mortuary handled around 600 deaths annually. In recent years, business had dropped off to around 300 per year.
It might not have mattered. With the March 2016 opening of Capitol Hill Station, transforming the blocks surrounding the busy transit center kicked into higher gear. The twin buildings set to replace Bonney Watson will join the four more under construction set to spring up around Capitol Hill Station.
UPDATE: Nora Menkin, executive director of 12th Ave's People's Memorial reminds us that there is still a local option:
The Co-op Funeral Home of People's Memorial located just across the park at 12th and Howell remains to serve Capitol Hill and greater Seattle/ King County residents. The Co-op Funeral Home has been here since 2007 and is the only cooperative funeral home in Washington State and the largest cooperative funeral home in the country. It is a one-time, lifetime member fee of $50 per person. While members receive a discounted rate on cremation and burial services, the funeral home serves all. In 2018, 869 families used our services making us one of the highest volume funeral homes in the city and state. Please let your readers know that they have us as a resource and option for their funeral plans.
The demolition involves one of the more significant recent efforts on the Hill. The latest wave of buildings to be torn down has mostly involved smaller structures. As with every time a building comes down in Seattle, the permitting process comes along with requirements related to materials and disposal. It also included a pest abatement program for the rodents living "underneath stumps, sidewalks and rocks" around the building.
The arrival of chain link fencing had cut down on people camping out around the building but didn't do much to deter taggers who seemed to increase their efforts in the former mortuary's final days.
The new Modera Broadway project from Mill Creek Residential will include around 200 apartment units plus a set of live/work spaces on the Cal Anderson side of the Weber Thompson designed project that are hoped to eventually house a few small businesses on the edge of the park. Maybe one can be a sales office for Capitol Hill human composting startup Recompose.
The Modera Broadway project will rise on both sides of E Howell with one building on the site of the surface parking lot to the north and the southern twin rising where Bonney Watson is being torn down today. While the nearby light rail station is part of the draw, the project will also include underground parking for around 100 vehicles. Modera Broadway — sorry, Modera Cap Hill was taken for a Denver project — will also harken back to this neighborhood's auto row history — not its mortuary past — for its design finishes and materials with a mix of brick and sheet metal.
$5/MONTH? SUBSCRIBE AND SUPPORT LOCAL NEWS: Support local journalism dedicated to your neighborhood. SUBSCRIBE HERE. Join to become a subscriber at $1/$5/$10 a month to help CHS provide community news with NO PAYWALL. You can also sign up for a one-time annual payment.


text:
Raffles Supplements Merdeka Bundle
The Merdeka Bundle is specially put together for people in their silver years and beyond. As your nutritional requirements change as you age, the bundle contains important supplements that cover important nutritional gaps to keep you healthy and active.
This bundle includes:
- 1 x Adult Multis 60s Expiry date: July 2021
- 1 x Bone Calcium 60s Expiry date: June 2022
- 1 x Glucosamine 1500 40s Expiry date: October 2020
Raffles Bone Calcium:
Calcium is the most abundant mineral in the body. Its primary function in cooperation with phosphorus is to help build and maintain bones and teeth. Calcium aids the blood-clotting process and helps control the passage of fluids through cells. It is also related to proper functioning of the heart and neuromuscular system. Magnesium is involved in muscle contraction and nerve function. Zinc is an essential trace mineral which supports the immune system.
Raffles Glucosamine 1500:
Glucosamine is a precursor for substances that provide the foundation for many of the body's tissues, including tendons, ligaments, cartilage and collagen. It stimulates cartilage cells to produce two proteins - proteoglycans and collagen - that help to hold joint tissues together. Glucosamine may also prevent the breakdown of collagen.
Glucosamine is produced in the body. However, as people age, their bodies become less able to produce glucosamine which result in their cartilage being less flexible. Glucosamine is not found in food and may need to be obtained through supplement.
Raffles Adult Multis:
Raffles Adult Multis is a broad-spectrum multivitamin that ensures you get the nutrients needed for optimal health. It contains essential vitamins, antioxidants, and minerals such as vitamins A, B, C, as well as zinc and iron. These support your immunity system, muscle function, and cardiovascular health. In addition, the multivitamin's time-released formulation allows the nutrients to be released into your body over an extended period for sustained nutritional intake.
|Bone, Joints, & Muscle, General Well Being


text:
Let SIMBA ADVENTURES Organize Your Dream Holiday
After breakfast meet your safari driver guide at the lobby and leave for Tarangire National Park with picnic lunch boxes for game drive. Tarangire is the fifth largest National park in Tanzania well known for its beautiful landscape and larger elephant population found nowhere on earth. Tarangire River and the majestic baobab trees dwarfing some animal species which comes underneath are among the highlights of this park. Tarangire – Lake Manyara ecosystem forms a cycle of annual migration of over 3000 elephant, wildebeest and zebras. Apart from elephant population you may expect to spot giraffe, buffalo, zebra, hippos, warthog & the three rare species of Lesser & Greater Kudu, Oryx and the Ash staling. Later drive to the campsite for overnight.
MEALS: Lunch & Dinner
ACCOMMODATION: At Campsite
After early breakfast, leave the camp and drive few minutes to the Ngorongoro main gate. Drive and descend into the crater floor for about 6 to 7 hours of game drive. This massive collapsed volcano is home to over 25,000 mammals ranging from the "Big Five" – lion, elephant, buffalo, rhino and leopard. The area also supports a vast variety of animals, which include herds of wildebeest, zebra, eland, warthog and giant hippos to some of the more than 500 bird species can be spotted in this area. Another big draw card to this picturesque place is its dense population of predators, which include lions, hyenas, jackals, cheetahs and the ever-elusive leopard, which sometimes requires a trained eye to spot. After an extensive game drive, you will leave the crater and drive back to your campsite for dinner and overnight.
MEALS: Breakfast, Lunch & Dinner
ACCOMMODATION: : At Campsite
After breakfast at the campsite, you will be picked up and drive few minutes to the Lake Manyara main gate. After completing all the payment formalities check in and begin your game drive to this wonderful park known for its tree climbing lions and over 400 of bird species, primate-filled forests and grassy plains. A large area of the park is covered by the alkaline Lake Manyara, the seasonal breeding grounds for large colonies of flamingos and many more waterfowls. The park is also home to giraffes, hippos, wildebeests, impalas and groups of noisy monkeys and baboons. After an extensive game drive leave the park and drive to back to Arusha town.
MEALS: Breakfast & Lunch
ACCOMMODATION: : At your own


text:
United Kingdom
United States
World English Go to Usborne.com
Usborne Quicklinks
Quicklinks for internet-linked books
Click on the links to visit the recommended websites.
Websites to visit
See butterflies up close
Watch butterflies visit flowers and see a caterpillar grow up into a butterfly.
Inside an ants' nest
See worker ants, baby ants and a queen ant in their nest.
Insect or stick?
Meet an insect that looks like a stick.
Listen to a cicada
What kind of sound does a cicada make?
Moth mask
Print out and colour moth and beetle masks.
Spider to colour
What colours will you use for the spider and its web?
Children, make sure you follow these three simple rules when using the internet:
For more tips, see Internet safety for children.
Adults - we recommend that children are supervised while on the internet. The content of a website may change at any time and Usborne Publishing is not responsible for content on sites other than its own.
For more on internet safety, see Internet advice for adults.
Websites with interactive content may not work on your tablet or smartphone, but you can view them on a computer. Find out more…
Creepy Crawlies
Which creepy-crawlies look like dead leaves? Can bugs live underwater? What happens to them in the winter? In this book, you'll find the answers and lots more about the fascinating world of insects. With simple text and stunning photographs and illustrations, plus links to websites with video clips and activities.
Websites do occasionally experience problems. If a link isn't working we recommend leaving it a while and trying again. If the site is still down the following day please report the problem using our contact form. We will fix the problem as soon as possible, or find an alternative link.
If this is the first time you have used Usborne Quicklinks and you can't see ANY links, you may need to adjust your web browser settings. To find out how to do this, see Help & Advice.
The links in Usborne Quicklinks may vary slightly from those described in your book because when a website closes down, or we find a better site, we update the links in Quicklinks. (If we remove any of your favourite sites let us know!)
To view and print out files in .pdf format, you need the free Adobe Reader software. Download Adobe Reader.
Sound files should play on a computer, tablet or smartphone. If you have difficulty, make sure you have the most up-to-date version of your web browser, or on a desktop computer, download the latest version of Adobe Flash Player (see Technical help).
To hear midi files, you need a free program such as Windows Media Player, Real Player or Quicktime. Make sure your speakers are switched on! For more about these programs, see Technical help.
© Copyright 2020 Usborne Publishing. Web design & Development by Semantic


text:
Report Ad
Expired! This ad has expired. TaDd98bH
$2700 One bedroom Townhouse for rent
- Price$0.00
Denver, Adams
Colorado, USA
Sublet.com Listing ID 2944979 Beautiful Fully Furnished Classy Condo includes, granite counter tops, cable, internet, upgraded front load washerdryer, professional monthly cleaning service and all utilities. It has also a secured entry way and ramp. This condo located on the first floor. Conveniently located in DTC at Inverness near Park Meadows Mall, Colorado Athletic Club, Inverness Golf Course, and Light Rail, Skyridge Medical Hospital. Near Corporate Verizon Data Center, Comcast, and Arrow Electronics.
- Posted on: 2017-02-28 07:02:59
- Classified ad views:
- Item ID: 7904095
Map location: 80221, Denver, Adams, Colorado, USA
Contact Seller


text:
- Guaranteed for 2 full months
- Pay by PayPal or Credit Card
- Instant Digital Download
Note: Today's train is running a little late, so you have less than 24 hours to grab these deals!
You know you took a great photo, but it's just not popping when you get it on your screen. You'd also rather be out taking more great photos than messing around on the computer any longer.
Today's delivery from The Photography Express is not one, but two amazing Lightroom presets deals to help you bring out the potential in your photographs in just one click.
Today you can pick up a bundle of three of our 101 Lightroom Presets packs for just $49 – or individually for $19 each.
Each pack contains 101 (or more) Lightroom Presets, carefully crafted by professional photographers to help you save time and get the look you want when post-processing photos in Adobe Lightroom.
Try out some sample presets with our sliders – one of our favourite transformations is "Big Colour Love".
Save $100 when you purchase the bundle for the next 24 hours only!
Already have all 303 of the dPS presets? Then check out this beautiful premium bundle . . .
Even more presets to help you create beautiful photos in Lightroom. This set of Andrew's Faded Glory, SuperBlack and Vintage Portrait Presets work in Lightroom 4, 5 and 6, Lightroom CC (2015), Lightroom Classic CC and the new Lightroom CC for mobile, with both JPEG and Raw files.
If you're wanting to experiment with:
then this premium bundle of presets will give you results in Lightroom with just a few clicks.
Save 58% for the next 24 hours only!
Have fun getting creative with your new presets – 2019 will be a fun year!
Both dPS and our trusted partner Andrew Gibson at The Creative Photographer offer 60-day money back guarantees, so you can easily buy now to secure the deal and try out the presets, and if you don't think they're for you, we'll refund you.
Don't miss the next two deals – sign up here for The Photography Express!
Disclosure: We receive a commission from our partners if you buy via our promotion, but it is at no cost to you. In fact, you're getting an even better price than usual!


text:
IHG Rewards Club has launched a new bonus point promotions for members residing in the Americas (excluding Quebec).
Members that stay two nights during the promotional period are eligible to earn 3,000 bonus points. This bonus promotions is open for all.
You can access and register for this offer on IHG's website here.
READ MORE: IHG Rewards Club Rate & Bonus Points And Miles Promotions
The current Accelerate promotion ends on April 15. This seem to indicate that there won't be global promotion before sometime in May. Perhaps there is separate offer for members residing in elsewhere?
Here are the terms and conditions of this offer:
Open to legal residents in North, South and Central America, who are eighteen (18) years old or older at the time of registration. Members from Quebec are not eligible for this promotion. Must be an IHG®Rewards Club member and must register member number in advance to participate in this promotion. 3,000 Bonus points will be awarded after member stays 2 qualifying nights during promotion period which is between 16 April 2017 and 15 May 2017 (both dates inclusive). Nights at the following hotels are eligible: InterContinental®, Crowne Plaza®, Hotel Indigo®, Holiday Inn®, Holiday Inn Express®, Staybridge Suites®, Candlewood Suites®, EVEN® or Hualuxe™ hotel worldwide. A night is qualified when paying qualifying rates, which include most business and leisure rates. Only one room per member per stay will be awarded the applicable night stay credits. No retroactive points will be awarded for stays prior to registration. Member will receive the 3,000 bonus points only once during this promotion. Allow 4-6 weeks from stay for bonus points to be posted to your account. Subject to standard IHG® Rewards Club terms and conditions and earning structure, which can be obtained by visiting www.ihgrewardsclub.com.


text:
How do I test the speed of my Internet connection?
There are many utilities and web sites that can be used to test the speed of your Internet connection. We recommend using a variety of speed tests in order to give you the most accurate information on your system performance. Our recommended speed tests are listed below:
Your speed results may vary slightly with the time of day due to the loading of the speed test servers. However, the average speeds reported using multiple tests should be within 5% of the advertised speed for your Internet connection. The tests should be run several times from different servers, observing the average results.
High Speed Internet For Rural Communities
Sugarloaf Network Systems © All Rights Reserved.


text:
For the first time in Poteau's history, the football team has won a state championship.
December 17, 2019
The state final game was held in Edmond at the University of Central Oklahoma stadium. It was only the fourth time Poteau has even competed in the finals.
Last year, the Pirates advanced to the semifinals before being defeated by Tuttle in Jenks. That was not enough for the team, though. They wanted to go all the way.
The pressure was on for the Pirates to win. Mayor White declared December 6th 'Beat the Eagles Day' and schools across the county sent cards to the football coaches. Despite the pressure, many players on the team felt excitement more than fear.
Many of the players were incredibly confident leading up to the big game, like seniors Easton Francis and BJ Kniesley.
"I wasn't really nervous. I took it as just another game," said quarterback Easton Francis. "just a bigger stage."
"It was more excitement than anything," BJ Kniesley, #7, said. "We had it in the bag."
However, some players, like Jaydin Mankin, did not feel similarly.
"I was 100% nervous," Mankin said. Mankin scored Poteau's only touchdown during the game. "The feeling was great, but [winning] it with my brothers was the best feeling," he said, regarding the moment he entered the endzone. To see more of the Pirate Log's interview with Mankin, click here.
Playing in a state championship can be intimidating, but Francis likes to get in the right headspace by listening to music. He also recommends this to other people.
"I just listen to music [in the moments leading up to kickoff]," Francis said. "Music should change people; it did me."
"The words '4A State Championship' will hype anyone up," said Kniesley of mental preparation.
When the team arrived back in Poteau, they were greeted by crowds of people lining the streets cheering for them. Community members held signs and banners expressing their pride. Police cars led the buses from Spiro to Poteau.
"It's like an army entering Rome," said Coach Mike Odom of the football team's final return to Poteau.
After arriving at the field house, Head Coach Werner personally presented each football player with a medal. Werner called out each of the 67 players' names by memory.


text:
Recent Posts
Monthly Archives: May 2014
I write to peel away the layers and find the words within I write and hope someone else out there will connect. I'm thinking of starting a new (another one!) blog without any "theme" except whatever. It would be called … Continue reading
Posted in Uncategorized Leave a comment
Low Day
Today I feel low. I try so hard. I judge myself. Never enough. What a waste of precious energy. My life is divine. Beautiful. A gift. I have so much. I am alive. I love the rain, the quacking duck that … Continue reading
Posted in Uncategorized Leave a comment
Positive Speech Day
The power of positive energy is pretty much a proven thing. I remember reading, in one of Robert Anton Wilson's books (Cosmic Trigger maybe?), "Positive energy is as real as gravity" and thinking yes, of course. However much we know … Continue reading
Posted in Positivity, Speech Tagged positive energy, Positive Speech Day, Robert Anton Wilson Leave a comment


text:
259 members17 Comments 22 Likes
788 members225 Comments 146 Likes
91 members9 Comments 10 Likes
252 members19 Comments 18 Likes
3188 members1704 Comments 708 Likes
Started this discussion. Last reply by soph Mar 24, 2015. 7 Replies 1 Like
Is there anyone here that I could talk to one on one… yeah this does have to do with weight and eating and restricting and binging and everything else that makes you go insane… and i feel like I'm…Continue
Started this discussion. Last reply by Jackie Sauless Mar 18, 2015. 3 Replies 0 Likes
Does anyone know what D is talking about here? "Come and share your story at the our 3 week training camp in Chang Mai. Learn how to train right and get the body you want and deserve." - he posted…Continue
Started this discussion. Last reply by Sam Swinea Feb 11, 2015. 3 Replies 0 Likes
Hey 30BAD family!I REALLY need your help. My boyfriend is the biggest meat and cheese lover in the world. he comes from a very authentic Italian family (his father even works at a meat packaging…Continue
Started this discussion. Last reply by Eric Farris Jan 5, 2015. 2 Replies 0 Likes
Hello everyone from the MIDWEST!!Sooo... I don't really know anyone from my area that is 801010 raw/rawtil4 or hcrv… so I was just thinking..Maybe some people from the Midwest would like to get to…Continue
Pleasure to be virtually acquainted, Jackie! Am glad that my post was some use to you. Haven't been too much around here lately, so apologies I haven't responded so promptly. (-: How is everything for you now? Would be glad to chat about starting to eat this way, it's certainly very difficult at first.
please e. mail or phone me about visiting the Quintessence farm in Costa Rica...
Hi! I used to live in Elmhurst =) but now i'm in the city! If you ever come down this way we can totally chill =)
© 2020 Created by TheBananaGirl. Powered by


text:
Want to know when Bordeaux Begles are next playing live rugby union on TV and on which channel?
This Bordeaux Begles Rugby Union TV Fixture Schedule is updated daily to make sure you know when and who they are playing on TV during the 2019/20 across all satellite and freeview channels in the UK such as Sky Sports, BT Sport, BBC, ITV and on official live streams.
So, If you are trying to find out if Bordeaux Begles are on TV today, tonight, tomorrow or this weekend make sure you view our Rugby on TV Listings for all of their upcoming live televised fixtures.
This Bordeaux Begles TV schedule was updated on 26 October 2020.


text: SMS Faculty Member Peter Asaro at Recent Panel for American Association for the Advancement of Science (AAAS) Feb 19, 2019 by mediastudiesfaculty in Faculty >>> SMS faculty member Peter Asaro was featured at a recent panel for AAAS. Go through these for coverage on the panel as well as direct quotes by faculty member Asaro himself. 1 2 3 4 Tagged with: faculty


text:
Christmas Celebration (via NewsLook)
Christmas is a happy, festive time filled with great spiritual significance, especially for the world's Christians. Festivities are held in many countries to celebrate the joyous occasion. Let's show you how different cities around the globe have marked the holiday — starting with caring for the poor…
great spiritual significance, festive time, joyous occasion, different cities, Festivities, L.A, Christians, caring, globe, Christmas, world, countries


text:
Sectional couches have the tendency to be big. If you have ever aimed to view a motion picture with the kids stretched out on the floor, or had company over and not had adequate seats for everyone, then you understand exactly what it is like to not have adequate space for individuals to sit. Granted, many kids choose to sit on the floor, however sometimes, if they had the option, they would rather be up on a seat Sectional couches merely provide more seating space. This means more individuals can be up off the floor, where they would probably rather be anyway. Which's how they fix the first ailment, which is not enough seating. Inflatable couches – these things are very underrated however they certainly work! They fit and are very low-cost. They are also ultra portable and can make for a great space saver. If you want to make use of the space occupied by the sofa, merely deflate it and you've got instantaneous space freed up. Use a matching inflatable bed and you're set! Inflatable stuff isn't for the pool only due to the fact that you can implement them inside your house, too!
You might find that your ferret likes to climb up into furnishings. Recliners are a favorite hiding locations of lots of ferrets so usage care when implementing your preferred chair while your ferret has to do with. They can be crushed by recliners and rocking chairs. A few ferrets will likewise try to climb up curtains and chew on cushions so it is best to watch on them while they are outside of their cage. As mentioned previously, lift recliner chair contemporary design chairs can be available in a variety of sizes. For utmost comfort, make it a point to understand just what size you require prior to buying one. Little sized chairs are made for individuals with a height of 5′ 4″ or less; medium sized chairs for individuals with a height of 5′ 4″ to 5′ 10″; and large sized chairs for individuals with a height of 5′ 10″ to 6′ 2″. If you are more than 6′ 2″ tall, there are unique recliner chairs that can accommodate your height too. There are likewise the additional large chairs that are made specifically for much heavier body sizes. These chair designs are few inches wider to accommodate up to 500 pounds of weight.
5 Hints About Recliners You Can't Afford To Overlook
– Dual Reclining Sofa, Dual Reclining Loveseat & Rocker Recliner at a really low-cost cost especially for this day. – Dual Reclining Sofa, Dual Recliners On Sale Edwardsville KS Loveseat & Rocker Recliner listed below Nowadays sofas leather recliner can be changed to match your favored seating positions. In some cases, these sofas also come with optional "pockets" for you to put your remote controls so you do not get up or reach too simply alter your TELEVISION or read another CD.
Recliners start at a few hundred dollars for entry level designs but pricier ones are a major financial investment not simply since they may cost as much as many countless dollars. A well made recliner may last you more than a few years and even as much as a decade. That is a long period in our modern fast paced lives and that kind of commitment takes some idea. Prior to you reach into your wallet and turn over your tough made money for an excellent recliner, it is best to understand precisely what the alternatives readily available to you are and be sure of what you want the recliner for. Figure out what kind of functions you want from your recliner, what kind of upholstery you wish to cover it with and in what colour and also whether you are going to put it in your house or office. Buying a recliner would be a welcome addition to your house. Among all designs, little recliners are pretty much popular as it occupied less area hence offer the same comfort and relaxation to your back. Having little house or a location where you wish to position these chairs, little recliner is the only alternatives left for you. In typical mode, these chairs work as couch and also gets thought about for interior decoration of the house. Let's quickly review a few designs of little recliner chairs.
Do Recliners On Sale Edwardsville KS Better Than Those Self Described Experts
Couches typically specify a 3 seated soft object commonly put in the living-room. The couch remains in general envisioned to be the made of leather or patterned upholstery. There are certain L" formed couches that complete the living-room to include convenience as lounge and recliners. Such a couch is referred to as Sectional couch which is consisted of a minimum of 2 and an optimum of sectional pieces. A sectional couch might have a curved as well as a circular arrangement. Available now at cheap cost, unique discounts and easy shipping. I am really satisfied with its qualities and recommend it to everyone desiring for a top-notch item with the most recent requirements at a cheap. You can read review from buyers to find out more through their experience. Catnapper Soother Power Lift Full Lay-out Oversized Chaise Recliner Chair – Autumn has actually worked advantageous for me and I think it would do wonders on you too. So why invest any more time? Enjoy it, you understand where you can buy the best ones.
Raise chairs are basically recliners, however with a few significant distinctions. Electric lane recliner with ottoman chairs are considered to be one of the most comfy types of recliner chairs out in the market today. Of all recliners are in the main relatively sturdy pieces of furniture with bases of wood or metal, and with leather, fabric or artificial upholstery.
Why Having High-quality Recliners Is Not Enough
Do they slump on the sofa; paper spread all over, getting ink on your valuable leather? A leather-covered sofa will with no doubt look lovely with whatever interior theme your house may have. For tropical climates, leather can be very hot and make you sweat more-it is non porous and gets sticky in hot climates.
Things To Instantly Do About Substandard Recliners
Now, before we enter why wood sofa tables are so pleasing to the eye, it is time to enter into a little detail about what they exactly are. You see, unlike a coffee table that is incredibly long and sits low to the ground or an end table that is smaller sized in size and a bit greater than a coffee table, sofa tables usually are taller and rectangle. Like, the will generally sit in between three to three and a half feet off the ground, sometimes they'll be taller, however that's not the standard, then will be longer in length, around two to three feet. The sizes of the couches can accommodate a little space or a large space. The size of the bed is dependent on the size of the sofa. A little sofa will become a twin size bed, while a large sofa will become a queen or economy size bed. Sometimes sectional sofa have mechanisms that latch together to produce a bed from the seating location. These beds have integrate in mattresses that likewise work as seating for the sofa.
Some of them are fortunate enough to be taken in and cared for by one of the handful of Lurcher Rescue organisations in the UK, run by kind, devoted people who move heaven and earth to discover undesirable Lurchers an excellent home (or as the soppy among us state, their "Forever Sofa"!). Not all stray or undesirable Lurchers are so lucky and for far too many of these sensational, stylish, affectionate and wonderful pet dogs, this implies a death sentence:-LRB- If the rest of your living room is lacking wood, however you like the concept of wood sofa tables, you need to come up with items to match your wood option up with. You can use the colors in any of these products to make sure that the wood sofa tables you are thinking about will be complementary to the general appearance of the room.
A Little Background Before I go any further, I believe a broad summary of Flexsteel would be helpful. Flexsteel Industries has actually been making and importing furniture considering that 1893-the exact same year the zipper was created. They are currently one of the largest producers in the market, and their products are sold the world over. Their furniture offers extremely well, which is why you see it in so many stores. Flexsteel has three furniture divisions: Hospitality, Vehicle Seating and Residential. The Residential division is more commonly referred to as Flexsteel Home Furniture and what I will talk about in this post. Flexsteel Home Furniture offers a wide variety of upholstered and wood furniture such as couches, loveseats, chairs, recliners, rocker-reclining chairs, swivel rockers, couch beds, occasional tables and desks. Leathher Recliners Reclimer Chairs Lazygoy Recliners Jedical Recliners Lazy Hoy Recliners Berkine Recliner Recliner Patrs Lane Rceliner Chairs Refliner Laptop Discountt Recliners Home Theate Rrecliner Reclienrs Recliner Office Chairr Rvv Recliners Massage Recilner Massage Recilner Small Reclinee Stressless Recliiner Recliner Contemporary Microfober Mocha Lane Reecliners Best Recliers Laane Recliners Online Lazy Bo Recliners Doulas Furniture, Taos Recliner Laether Recliner Sofas Ower Recliner Zero Gravity 4ecliners Lazy Hoy Recliners Lane Reecliners Lazy Boy Recilner Lazy Hoy Recliners Lazy Hoy Recliners Power Reciner Medica Lrecliners Lane Rceliners Recliner Cove4s Leather Recoiner Lazy Hoy Recliners
Excellent Producers Of Recliners In Your City
Pros: We don't simply sit in chairs or lounge in recliners. When we remain in our beds, we are not lying down all the time. We sit in many different places, and for those who suffer from persistent pain in the back, sitting easily is generally the biggest issue. They can stand, stroll, or lay relatively easily, however finding a comfortable way to sit is a difficulty. The Bedlounge Classic is created making any sitting situation more comfy, whether you are sitting in a chair or propped up on your bed viewing TELEVISION or checking out the most recent "whodunit." Riser Recliners are fantastic due to the fact that they are basically reclining relaxer chairs, simply with an added perk. Unless you own one yourself, it's challenging to tell a riser how to sleep in a recliner from a regular chair. This implies you get the overall comfort anticipated of a relaxer chair; the kind of comfort that makes you never wish to stand up. There is no loss of quality on riser recliners, infact most comfort chair makers make riser variations of their chairs, for example Lazy Boy.
Fix The Way You Think About Recliners On Sale Edwardsville KS
If you remain in the market for a new living-room or living room set, there are a couple of options on the market for you to think about. The most evident is a couch, a love seat and a chair set. Other options include a hide-a-bed or clearance sale on recliners instead of a conventional couch or chair. Another terrific choice are sectional couches. A sectional is made up of several sections (thus, "sectional") of furniture, that fit together to form one big piece of furniture. Let's take a look at the different pieces readily available, the function they serve and why a sectional may suit your life. The sectional sofa is one that will be linked, linking lots of seating locations in the house into one long location. The sectional sofa is one that can complete the house, giving all the household a place to sit, and that will be comfortable at the very same time. Sectional couches are now readily available for purchase online, and you can have them delivered straight to your house without delay. No need to go shopping and waste your gas, you can take a look at, examine the pictures, and find the products that are going to fulfill your every expectation online.
Finding the best furniture for the den will likely be the trickiest part. Factors to consider of the room's usage will be essential. Will there be a couch for sitting and enjoying the television? Should it be a sofa couch so overnight guests have a room all their own? Does the room require a desk? By identifying all the furniture pieces that everyone will require in the room and after that acquiring coordinating or matching pieces will help to bring all the room's functions together into an ideal design. That is obviously, if you mind the size of the room and keep the variety of furniture pieces and size of the furniture in the proper proportion while keeping a great room flow. Production a white leather sofa is no big deal. When the leather is processed for making a white sofa it is colored white. The value of proper dyeing is enormous due to the fact that you do not want the white colour to leave the sofa and make marks on individuals's clothes. This is the factor you need to always buy this modern leather sofa just from well recognized producers and sellers.
Prior to you purchase and place your leather furniture in your house, aim to envision the future. Leather furniture is universal and timeless in lots of regards, keep in mind that it will require special care and placement. Keeping that leather sofa far from the window and the heating system and rather finding the ideal accent lighting and a toss blanket might be the very best bet. Find an excellent source for designer lights and décor and strategy your space long before you make you very first purchase. You'll thank me in twenty years. Get now 3pc Traditional Modern Fabric Sleeper Sofa Set, CO-CIN-S2 on highest and the great price today. I am extremely pleased with the qualities and highly advise it to all individuals searching for a quality product with the beneficial functions at an low. You can check out testament from individuals to discover more from their experience. 3pc Traditional Modern Fabric Sleeper Sofa Set, CO-CIN-S2 has actually worked useful for me and I want it would do marvels on you too. So why waste your time? Enjoy yourself, you understand where to obtain the very best ones.
Trouble-free Recliners That You Will Love
Place a dog crate on the floor by the recliner and fill it with books so a Recliners On Sale Edwardsville KS read is constantly within arm's length. For last so numerous years, different types of couch sets are implemented as a popular furnishings product for house or workplace. A recliner couch is specifically made to support your lower back at a perfect angle for optimum comfort and unwinding posture while sitting.
Why do you think a leather sofa has such appeal to so lots of people? What intrigues me most about a leather sofa is the truth that it does have such a strong pull on lots of people. It's almost a psychological reaction that people get when they experience a leather sofa.
Recliner couches have ended up being an invited piece of furniture in any family's living space. These types of couch recliners are also called riser recliners. An example is when youngsters jump in consistently on the recliner, or when a huge man sits on a small recliner or when a lot of unneeded things is positioned on the recliner negligently.
People Love New Recliners
There are essential factors to consider to make before selecting the ideal reclining seats in movie theater. Let's look at some of the terrific factors to acquire these recliner items. The legs of sofas, recliners and beds can easily wear away or tear weak products (such as balloons) as certainly they weigh a reasonable bit, and the edges of the legs have the prospective to dig in.


text:
- Home
- Inspiration
- About us
- Products
- Ontario Locations
Due to COVID-19 the Ensuite will not be meeting with customers in person. We do offer phone, email or Facetime consultations to help you with your selections. Thank you for your understanding at this time.
Please note that many showrooms have reduced hours or are open by appointment only, please contact each showroom directly for more information regarding operating hours.
Let us help you choose the ingredients that make for a successful new kitchen or kitchen renovation project. Classic and traditional to contemporary and cutting edge, The Ensuite has the products that can truly make your kitchen the heart of your home. Book a one-on-one consultation at your local Ensuite location today.


text:
McAfee study: 1-in-4 organisations using Public Cloud has had data stolen
McAfee's third cloud security report finds lack of adequate visibility and control as the greatest challenge to cloud adoption in an organisation.
Top 10 tips on keeping your cloud options open: The importance of choice
Businesses in Asia Pacific are rapidly embracing cloud infrastructure (IaaS), with nearly half agreeing that IaaS will have a role to play in their business within three years.
The journey to the cloud is only just beginning
Cloud computing continues to drive incredible transformation. It has changed the way we run businesses; creating whole new sectors and disrupting entire industries.
Oracle to set up its Digital Sales Hub in Malaysia
Plans to hire 200 employees for the Oracle Digital Sales Hub The hub, located in Kuala Lumpur, will serve customers from 23 countries ORACLE Corp, one of the top software companies globally in terms of revenue, has decided to build one of its five state-of-the-art Digital Sales Hub in Malaysia.
Mediatrac wants to create a big data wave in Indonesia
Jakarta-based BDA company Mediatrac wants to bring the big data wave to Indonesia with its inhouse technology – but in the proper manner, say senior executives.
Asia's public cloud spend to double, outpacing overall IT spend by 6x
Spending on public cloud services in the Asia Pacific region excluding Japan (APeJ) will grow at a 22.9% CAGR from nearly US$7 billion in 2015 to US$15 billion in 2019, according to IDC.
Tunjuk bos baru, Oracle fokus pada bisnis cloud
Sejumlah perusahaan kian sadar pentingnya berinvestasi di bidang IT Komputasi awan bisa menekan biaya operasional perusahaan
Oracle gets aggressive about its cloud business
Software giant Oracle Corp has aggressive plans to grow its cloud business in Asia Pacific, including South-East Asia, reports Goh Thean Eu.
IBM announces new enterprise IoT unit
IBM has announced that it will invest US$3 billion over the next four years to establish a new Internet of Things (IoT) unit and build a cloud-based open platform to help clients and ecosystem partners across industries better integrate real-time data and insights from dispersed sources directly into business operations.
Singapore startup Skoolbo out to boost education for millions worldwide
Singapore startup Skoolbo said its game-based education platform, which runs on Microsoft Corp's Azure PaaS (Platform-as-a-Service) offering, has reached more than five million students in 22,000 schools globally over the past 12 months.


text:
This is a prophetic word that I have not been able to escape, the Lord has continued to confirm this word with multiple uncanny and unusual ways (even again this morning, as I awoke to the Lord saying "I want you to release this word to the body with the purpose to give strength and encouragement").
In "1 Kings 18:16-45" we read of the story of Elijah and how he challenged the 450 prophets of Baal to a showdown of power and demonstration. As we know there was no answer from Baal, though the God of Elijah answered with fire falling on the alter that Elijah had rebuilt (consuming the sacrifice, the wood, the stones, the soil, and even the water that lay in the trench. The water which Elijah had ordered three times to be poured over the alter). Then in verse 45 of 1 Kings 18, we read that the three year drought is broken with a heavy rain.
As the Lord began to speak to me about Elijah, I was reminded about how "Gabriel" the angel of the Lord (in Luke 1) came to Zechariah and spoke to him concerning the birth of his son John, and how he will be a man with the spirit and power of Elijah, preparing the people for the first coming of Jesus.
So heres' the word:
Just as Elijah baptised the alter of the Lord with water and the fire fell, John (who carried the spirit and power of Elijah) came baptising in water with the message of Mat 3:11 "...one is coming who is greater than I am...He will baptize you with the Holy Spirit and with fire".
Though this time the fire in not falling on an alter made by human hands (it's a new covenant fire), it's a spiritual fire falling on you and I (living stones). 1 Pet 2:5 "You also are like living stones, so let yourselves be used to build a spiritual temple—to be holy priests who offer spiritual sacrifices to God".
I heard the Lord say He is raising up an Elijah generation, a company of forerunners who live in the spirit and don't bow to the enemies intimidation, a generation of no compromise who disrupt the status-quo, a generation full of faith and without fear who are bold in the demonstration of the Lord, a generation who like John will be a prophetic voice declaring the second coming of Jesus (which is already here).
As the fire falls the drought will break:
The Lord is desiring right now to baptise his bride with the Holy Spirit and with fire, setting a blaze sons and daughters. As we position our hearts and spirits for a fresh baptism of fire, there will come a proceeding rain that will bring life to any drought. As the fire falls on you and I, so comes an outpouring over regions, cities, ministries, families, churches, nations, etc. We will begin to speak into circumstance and situation, releasing a refreshing as we ourselves have been refreshed. You are the drought breakers....this is the reason you are set a light!
Jason Harrison
Bendigo - Australia