text:
S ATP synthesis, which explained the increase of ADP content that was expected to be the result of the disturbance of synthesis of ATP. In addition, lower levels of glycogen and Na+ +-ATPase activity as well as the higher lactic acid content caused by the acidism and glycolysis in the lung tissue confirmed that LIRI would cause evident energy metabolic disturbance. RvD1 can protect the structure and function of mitochondria and can further maintain the function of oxidative phosphorylation, promote ATP synthesis and delay the depletion of ATP, and finally recover the ratio of ATP to ADP. Meanwhile, it can enhance the activity of Na+ +-ATPase to balance the homeostasis of the cells. Additionally, through up-regulating the glycogen content and down-regulating the lactic acid level, the energy metabolism was improved remarkably by RvD1, resulting in the less damage of the lung tissue when experiencing LIRI. Sukoyan et al. [49] agreed that the LIRI can be alleviated through improving cellular energy metabolism, such as protecting the mitochondrial oxidative respiratory activity, enhancing the ability of mitochondrial oxidative phosphorylation function and generation of high-energy phosphate compounds, as well as making the cells utilize the highenergy phosphate compounds. Ying et PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28893839 al. [50] thought that mitochondrial PD173074 site dysfunction was an important cause of IRI and the key factor to initiate apoptosis. This experiment is also observed RvD1 can reduce IR-induced apoptosis by improving energy metabolism and mitochondrial function, There is still no clear evidence on whether RvD1 functions in the part of the energy metabolism, in a direct or indirect way. The relationship among the energy metabolic disturbance, inflammatory response and the oxidative stress response should be mutual connected and influenced as well as reciprocal causation. LIRI, characterized by inflammatory reaction and oxidative stress, can destroy the balance of the oxidative/anti-oxidative and pro-/anti-inflammatory systems. Many studies confirmed that the secretion of the inflammatory cytokines TNF-, MIP-2, MIP-1, MCP-1 and CINC-1 would be increased during LIRI, which benefited the recruiting of neutrophils and further promote lung injury [51, 52]. In our study, we got the similar results. In particular, LIRI led to the large increase of the serum levels of IL-1, TNF, IL-10 and the concentrations of MCP-1, MIP-2 and CINC-1 in the lung tissue. In addition, more numbers of neutrophils and aggressive lung damage were observed in the lung tissue. Inflammation is associated with an oxidative stress reaction, which is produced in the development of inflammation, that which has positive feedback on inflammation itself [53]. Oxidative stress is consideredto be an important pathogenic event in IRI. The deleterious effects of LIRI are in part mediated by the formation of free radicals and super oxides [54]. When the body suffered from ischemia, the function of the oxygen free radical scavenging system would be reduced or even totally loss; however, the activity of oxygen free radical generation system in the other way is enhanced. Once the blood supply is recovered, the oxygen free radicals would accumulate rapidly and further cause the damage of the tissue [55]. The scavenging of the free radicals is mainly achieved by a series of antioxidant enzymes, such as SOD and GSH-PX. Our experimental results showed that LIRI cause the imbalance between the production and elimination of the fr.
Month: May 2018
Nd 5-GCC TTA CAC CCA GGT CTC TG-3 for c.460G>A), 5 l DNA and 1X
Nd 5-GCC TTA CAC CCA GGT CTC TG-3 for c.460G>A), 5 l DNA and 1X of PCR master mix (promega, USA). The cycling conditions consisted of 35 cycles of 94 for 1 min, 56 for 1 min, 72 for 1 min for c.719A>G; and 35 cycles of 94 for 1min, 59 for 1 min, 72 for 1 min for c.460G>A.Data collection and analysisThis is a descriptive study with Convenience sampling and definite time period. Fifty six children suffering from and being managed for acute lymphoblastic leukemia in European Gaza hospital, AL-Nasser hospital and Abd El Aziz Al Rantisi hospital were included in the period between July 2008 to august 2009. The study population included all patients attending these hospitals at time of the study. Other types of leukemia were excluded. Approximately 2.5 ml venous blood samples were collected, from the patients in EDTA tubes. The procedures of the study were approved by the Helsinki research ethics committee of the Palestinian authority ministry of health according to the World Medical Association Declaration of Helsinki [11], and a written consent was obtained from the parents of each patient.TPMT genotypingRelevant medical information was collected from the patient's records in the hospitals. Data included adverse effects such as leucopoenia (white blood cells count <3000/ cubic millimeter), thrombocytopenia (platelets < 100000/ cubic millimeter), abnormal liver function (elevation of ALT level of 2 or more times the upper limit of normal); and duration of 6-MP therapy at time of sampling and at the time of adverse effects appearance. Data were analyzed using the SPSS software (Version. 17) as needed. Chi square test and One-Way ANOVA were used and P-values of < 0.05 were considered statistically significant. Means were presented ?standard deviation.ResultsDescription of the study populationDNA was extracted from 300 l whole blood samples using a commercial kit (promega, USA), according to the manufacture recommendations, and based on nonorganic extraction procedure. The extracted DNA was resuspended in 100 l of the provided alkaline hydration solution at 65 . A total of 56 DNA samples were analyzed. Total genomic DNA extracted from peripheral leucocytes was processed by PCR either immediately or stored at -20 until being used. An allele-specific PCR [12] was used to analyze the mutation c.238G>C in exon 5 (TPMT *2 allele), using sequence specific primers (wild type specific: 5-GTA TGA TTT TAT GCA GGT TTG-3, mutant specific: 5- GTA TGA TTT TAT GCA GGT TTC-3 and common: 5-TAA ATA GGA ACC ATC GGA CAC-3) in two separate 20 l reactions containing 0.5 M of each primer, 5 l DNA 1X PCR master mix (promega, USA).In this study, the sample included 56 pediatric patients suffering from ALL; 32 (57.1 ) were males and 24 (42.9 ) were females. Their ages at the time of diagnosis varied between 6 months to 12 years (mean 4.4 ?2.6 years). The majority of patients were between 3.7 – 5.1 years with 95 Confidence interval. All patients were diagnosed as ALL patients by blood film, bone marrow aspiration and complete blood cell count (CBC). In the present study the calculated AZD0156 cost incidence of ALL among children in Gaza strip during the specified period of samples collection was 2 patients per 100000 children. The incidence of ALL is higher in males than in females.Management of patientsDuring the collection of the sample, most of PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27735993 the patients (75 ) were given 6-MP for different periods of time and 25 of them finished having the 6-MP treatment.Ayesh et al. B.
The final manuscript. Acknowledgements We thank the BC Children's Women's Hospital staff for
The final manuscript. Acknowledgements We thank the BC Children's Women's Hospital staff for their help with subject recruitment; Ruby Jiang, Mihoko Ladd, Paul Villeneuve, Drs. Julie MacIsaac and Maria Pe herrera for their work in sample processing, and Dr. Lisa Xu for flow cytometer operation. This research was funded by grants from the Canadian Institutes of Health Research (CIHR; MOP-123478 to PML and MOP-49520 to WPR). OMdG is T0901317MedChemExpress T0901317 supported by a CIHR Frederick Banting and Charles Best Graduate Scholarship–Master's Award. HRR is supported by a fellowship from the Mitacs national research organization. EMP is supported by a CIHR Frederick Banting and Charles Best Canada Graduate Scholarship–Doctoral Award. MJJ is supported by a Mining for Miracles fellowship from the Child and Family Research Institute (CFRI). MSK is the Canada Research Chair in Social Epigenetics. PML is supported by Clinician-Scientist Awards from the CFRI and the Michael Smith Foundation for Health Research (MSFHR). WPR is supported by an investigator award from the CFRI. Author details 1 Child Family Research Institute, 950 W 28th Avenue, Vancouver, BC V5Z 4H4, Canada. 2Department of Medical Genetics, University of British Columbia, Vancouver, BC V6T 1Z3, Canada. 3Department of Pediatrics, University of British Columbia, Vancouver, BC V6T 1Z3, Canada. 4Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada. 5Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, Vancouver, BC V5Z 4H4, Canada. Received: 30 June 2015 Accepted: 31 AugustAdditional fileAdditional file 1: Supplemental Methods and Data. This contains Supplemental Methods, Supplemental Tables 1 and 2 and Supplemental Figures 1-5.Abbreviations 450K array: Illumina Infinium HumanMethylation450 BeadChip; DM: differentially methylated; DNAm: DNA methylation; FACS: fluorescence-activated cell sorting; FDR: false detection rate; NK cells: natural killer cells; nRBC: nucleated red blood cell; RBC: red blood cell; SV: surrogate variable; WBC: white blood cell.References 1. Chen L, Kostadima M, Martens JH, et al. Transcriptional diversity during lineage commitment of human blood progenitors. Science. 2014;345(6204):1251033. 2. Ji H, Ehrlich LI, Seita J, et al. Comprehensive methylome map of lineage commitment from haematopoietic progenitors. Nature. 2010;467(7313):338?2. 3. Laslo P, Pongubala JM, Lancki DW, Singh H. Gene regulatory networks directing myeloid and lymphoid cell fates within the immune system. Semin Immunol. 2008;20(4):228?5. 4. Simon LM, Edelstein LC, Nagalla S, et al. Human platelet microRNA-mRNA networks associated with age and gender revealed by integrated plateletomics. Blood. 2014;123(16):e37?5. 5. Lam LL, Emberly E, Fraser HB, et al. Factors underlying variable DNA methylation in a human community cohort. Proc Natl Acad Sci U S A. 2012;109 Suppl 2:17253?0. 6. Reinius LE, Acevedo N, Joerink M, et al. Differential DNA methylation in purified human blood cells: Implications for cell lineage and studies on disease susceptibility. PLoS One. 2012;7(7):e41361. 7. Whitney AR, Diehn M, Popper SJ, et al. Individuality and variation in gene expression patterns in human blood. Proc Natl Acad Sci U S A. 2003;100(4):1896?01. 8. Houseman EA, Accomando WP, Koestler DC, et al. DNA methylation arrays as surrogate measures of cell mixture distribution. BMC Bioinformatics. 2012;13:86. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27864321 -2105-13-86. 9. Houseman EA, Molitor J, Marsit C.
The new Presidential initiative on precision medicine in the United States [1]. It is obvious
The new Presidential initiative on precision medicine in the United States [1]. It is obvious to the readers of BioData Mining that this will require careful analyses of large and often complex data sets to best translate information into increasingly individualized risk. Here we ask why improved and appropriate data mining is not only positive but a vast improvement on most current analyses of genomic data. The answer lies to some extent in elucidating the present practice of -omic analyses and how we will need to expand it. Many current -omic approaches rely on univariate and linear analyses that can often miss the underlying architecture of complex traits. For example, univariate analyses of single genetic markers for association with disease risk, prognosis, or drug response that are the analytical standards for genetic analyses of human disease, and have been promoted as a means to develop personalized or more recently precision medicine, make many assumptions about architecture. Given the interest in precision medicine, it is important to ask explicitly what is being assayed in these types of studies that have been argued, incorrectly we believe, as the precursors to precision medicine. Most human geneticists study the association of genetic variants, be they common or rare, assessed across moderate to large samples of cases and controls. The effect of each allelic substitution is then measured as it associates with a particular purchase ONO-4059 phenotype. These estimates can provide useful population level risks; however, they are simply the average effect of an allelic substitution across the population, not necessarily predictive of results in an individual or a subgroup. The concept of average allelic effect is one that is well developed in quantitative genetics, but by its very name is suggestive not of precision medicine but of average medicine. Hence, it is possible in a large outbreeding?2015 Williams and Moore. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Williams and Moore BioData Mining (2015) 8:Page 2 ofpopulation with multiple types and levels of environmental exposure that an average odds ratio can have large variances, which are often reported as 95 confidence intervals. However, some people with a "risk allele, as defined by the population average, will not have an increased risk. At the extreme it is even possible to have opposite effects. For example, it is well recognized that increasing PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28298493 levels of salt intake can on average result in higher blood pressure and subsequent sequelae, including end stage organ disease. But as much as 10 of some populations may have inverse salt sensitivity or an increase in blood pressure with decreasing salt intake. This illustrates that lowering salt is not a universal good [2]. On top of this there is some evidence that different genes associate with inverse salt sensitivity than those that associate with canonical salt sensitivity, making direct comparisons impossible [3]. Of course, this limitation is not unique to genetic analyses as environmental exposures are also usually dealt with.
Al chimeric receptor ErbBgp expressed around the cell surface and induceAl chimeric receptor ErbBgp expressed
Al chimeric receptor ErbBgp expressed around the cell surface and induce
Al chimeric receptor ErbBgp expressed on the cell surface and induce cell proliferation signaling in the dimerized chimeric receptor, were investigated. The results showed that the fusion protein together with the hinge linker was the top for activating ErbBgp chimerainduced cell proliferation . It has been demonstrated that the selective complex formation of Pcam with its redox companion proteins, PdX and PdR, might be accomplished by fusing each element for the Cterminus of a diverse subunit of theheterotrimer PCNA from Sulfolobus solfataricus to type a selfassembling scaffold . To enhance the ON 014185 supplier activity of this selfassembled multienzyme complex, the peptide linker connecting PdX with PCN was optimized making use of various peptide linkers, including versatile linkers (GS)n , helical and rigid Prorich linkers (GSP)nGS) as well as other linkers (GS VPRGS S). While the activity was affected by the lengths of each the rigid Prorich linkers along with the versatile linkers, the Prorich linkers offered the greatest activity enhancement. The optimized Prorich linker (GSP) S) enhanced the activity by .fold compared together with the GS VPRGS S linker, although the (GS)n linker did not yield activity higher than the maximum activity in the optimized Prorich linker. Both peptide linker rigidityflexibility and length had been located to become essential for enhancing overall multienzyme complicated activity (Fig.) .Fig. Optimization of your PCNAPdX fusion protein linker in PUPPET. a Pcam oxidation activities on the PUPPET linker variants, PUPPETPn . b Pcam oxidation activities of the PUPPET linker variants, PUPPETGn (n ). c A docking model of Pcam and PdX. d Spatial arrangement of Pcam as well as the PCNA ring when the PdXbinding site of Pcam faces in the identical path towards the PCNA ring. e Spatial arrangement of Pcam and the PCNA ring when the PdXbinding site of Pcam faces in a perpendicular direction to the PCNA ring (Figures reproduced from Ref.)Nagamune Nano Convergence :Web page ofThe tandem fusion proteins glucanase (Gluc) xylanase (Xyl) have been constructed applying peptide linkers, including versatile linkers (GS)n , helical linkers (EAK)n and other individuals (MGSSSN made using the software of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26296952 the net server LINKER , and TGSRKYMELGATQGMGEALTRGM derived from the two helix bundle of Humicola insolens endocellulase). The effects of your linkers around the thermal stability and catalytic efficiency of both enzymes were analyzed. The Gluc moieties of most fusion constructs showed higher stability at than did the parental Gluc and also the linkerfree fusion protein. All the Xyl moieties showed thermal stabilities simi
lar to that of your parental Xyl, at . It was also revealed that the catalytic efficiencies from the Gluc and Xyl moieties of all of the fusion proteins were . to .fold and . to .fold those of your parental moieties, respectively. The versatile linker (GS) resulted in the ideal fusion proteins, whose catalytic efficiencies have been increased by .fold for the Gluc moiety and by .fold for the Xyl moiety. The Gluc and Xyl moieties with the fusion protein with the rigid linker (EAK) also showed . and .fold increases in catalytic efficiency . Aiming to clarify the criteria for designing peptide linkers for the productive separation of your domains inside a bifunctional fusion protein, a systematic investigation was carried out. As a model, the fusion proteins of two Aequorea GFP variants, enhanced GFP (EGFP) and enhanced blue fluorescent protein (EBFP), were employed. The secondary structure of the linker plus the relative distance in between EBFP a.
Ined use and irrespective of whether a single supplement negated the impact of theIned use
Ined use and irrespective of whether a single supplement negated the impact of the
Ined use and whether or not one particular supplement negated the impact in the otherStudySubjects and designaBicarbonate and caffeineFelippe et al. Regional and national level judo players (n M)Crossover style to create bicarbonate, caffeine, combined, and placebo trialsChristensen e
t al. International level rowers (n M, F; lightweight and heavyweight)Crossover style to create bicarbonate, caffeine, combined, and placebo trialsKilding et al. Welltrained cyclists (n M)Crossover design to create bicarbonate, caffeine, combined, and placebo trialsL. M. BurkeTable continued Dose Functionality measure Overall performance MedChemExpress Docosahexaenoyl ethanolamide benefit BicarbonateNo CaffeineYes Interactionindependent mechanism with counteractive effect (indirect) SummaryStudySubjects and designaCarr et al. m ergometer TT Overnight fastedWelltrained rowers (n M, F)Reallife Utilizes of Efficiency SupplementsCrossover style to make bicarbonate, caffeine, combined, and placebo trials mgkg sodium bicarbonate min preexercise andor mgkg caffeine min preexerciseRowingPerformance was substantially enhanced by caffeine (:. min:s) compared with placebo (:.) but differences between bicarbonate (:. min:s), combined (:. min:s) and placebo have been unclear. GI symptoms connected with bicarbonate brought on failure of anticipated efficiency as a result of enhanced buffering, but in addition counteracted the added benefits of caffeine. Note that protocol didn't make use of techniques to decrease GI effects of bicarbonate.Pruscino et al. Swimming m min recovery Competition components built into research style (warmup, racing against others and so on.)Highly trained swimmers (n M)Crossover design and style to generate bicarbonate, caffeine, combined, and placebo trials mgkg sodium bicarbonate spread min preexercise andor mgkg caffeine min preexerciseBicarbonatePerhaps CaffeineNo, possibly harm Interactionindependent mechanism with unclear additive effect (indirect)Variations amongst trials for absolute times failed to attain statistical significance. However, magnitude based inference analysis showed usually trivial effects apart from caffeine exactly where effects ranged from trivial effect to large harm. bicarbonate showed a smaller reduction in swimming time from Swim to Swim (quicker) than caffeine or combined trials (p). Effect was significantly less evident for a single work. Majority of athletes recorded quickest TT for single and repeat overall performance from the combination of bicarbonate and caffeine. Authors recommended that caffeine could have advantage for 1st swim but this had adverse carryover impact on second swim that was overcome by bicarbonateSSTable continued Dose Overall performance measure Efficiency benefit SummaryCycling km ergometer TT Interactionindependent mechanism with no additive effect CaffeineYes . mmolday nitrate in BJ . h preexercise andor mgkg BM caffeine h preexercise NitrateNo Caffeine alone and combined resulted in improved energy output (p) compared with placebo on the other hand variations amongst these trials were not substantial. Time to complete the . km distance was lowered to a equivalent extent (p) for both the combined and caffeine trials. BJ had no considerable constructive or negative effect on time for you to comprehensive the TT. Employing an inference based statistical strategy. Caffeine would likely and extremely probably improve TT functionality for caffeine and combined, respectively. BJ was unlikely quite unlikely to possess any PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/22298589 positive impact on functionality for BJ and combined, respectively Cycling . ergometer TT simulating Olympic course Nutritional situations simulating race prac.
Faso have revealed that young folks (in between ages and)were much moreFaso have revealed that
Faso have revealed that young folks (in between ages and)were much more
Faso have revealed that young individuals (in between ages and)were extra willing to spend as compared to the older men and women. At the household level, older age of household head was positively related with enrolment in Ghana, Mali and Senegal . Some studies conducted in Burkina Faso and India showed that urban dwellers had been willing to spend much less as compared with rural dwellers although the opposite was recorded in an additional study performed in Burkina Faso . Education also played a important role in uptake of CBHI, as all studies carried out in Nigeria, Ghana, Mali, Senegal, Burkina Faso, India and Malaysia that reported this variable discovered that the less educated were prepared to pay significantly less compared to the much more educated , at each household and individual levels. The research measured willingness to pay as opposed to the capability to pay, although the former might be employed as proxy to measure the latter.Wealthier households and men and women (richest quintile or as defined by the study) had been additional prepared and capable to pay more for well being insurance coverage than the significantly less wealthy as seen in studies carried out in Cameroon, Burkina Faso, India, Nigeria and Malaysia However a single study carried out in Nigeria reported differently when it comes to wealth quintile and enrolment whereby these with high income have been much less most likely to spend than these with reduced earnings . Findings from qualitative studies also show that wealth quintile was stated as a sociodemographic aspect revolving about the uptake from the scheme, and as shown by quantitative studies, affordability can be a crucial element affecting enrolment. NonenrolledAdebayo et al. BMC Well being Solutions Research :Page ofindividuals collectively identified a lack of economic implies as the key purpose for not enrolling in Burkina Faso and Uganda (Further file Table S). Moreover, household PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26544124 size was a further important element that was discovered to affect uptake of CBHI schemes. Research conducted in India and Nigeria found that bigger households (six members and above) were prepared to pay greater amounts than somewhat smaller households . This differs from what was reported in some other research performed in Burkina Faso and India . Exactly where bigger households dropped out from the scheme, this was most likely as a result of the substantial economic burden faced by households when they seek well being care. Some research carried out in Nigeria and Malaysia associated marital status for the uptake of the scheme. Single folks had been more willing to spend than married couples Households that were members of an existing association in the community have been extra prepared to enrol in to the VEC-162 chemical information scheme as observed in Cameroon which reveals the function of solidarity and social cohesion on willingness to spend for the scheme.Wellness related variables influencing uptake of CBHISummary final results for overall health connected things influencing the uptake of CBHI are presented in Fig. and Added file Table S. The high quality of wellness care is yet another key issue that was located to influence the uptake from the scheme. Folks or households that perceived high-quality of care as good were identified to become a lot more willing to spend tha
n those who perceived the top quality with significantly less admiration as reported in Burkina Faso and Nigeria 1 study carried out in Nigeria linked the top quality of wellness care and distance together inside the sense that, households that perceive high-quality of overall health care centres close to them as poor are willing to enrol in to the scheme and are willing to spend higher . This would enable them have access to other facilities that.
Promise to identify novel targets for therapeutical intervention. In this paper we have discussed the
Promise to identify novel targets for therapeutical intervention. In this paper we have discussed the problem of comparing two labelled networks that are representative of two conditions (e.g. healthy and diseased tissues) and detecting statistically significant differences in their topology. Identifying disrupted interaction patterns in two labelled network comparisons is a challenging problem requiring novel statistical tools, and three contributions have been made here in this direction. Firstly, we have proposed the GHD, a distance between two labelled networks that detects more subtle differences compared to the traditional Hamming distance. Secondly, we have demonstrated that the GHD can be used as a non-parametric test to assess whether two purchase Nutlin-3a chiral paired networks are statistically independent, and have described how p-values can be computed in closed-form without requiring computationally expensive permutation procedures. The plausibility of the conditions underpinning our derivations has been discussed using scale-free random network models as an example. Thirdly, we have proposed a fast subnetwork detection procedure, the dGHD algorithm, PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/26780312 to detect localized topological differences between two paired networks. This methodology provides a usefulFig. 8 Visualization of the distribution of differential methylated probes (red) in differential subnetworks detected by dGHD in the DNA co-methylation networksMontana et al. BMC Bioinformatics (2015) 16:Page 12 ofTable 2 DNA co-methylation networks: a summary for different communitiesC# of probes qi Ri BP MF KEGG 418 4 .181 320 54C66 66 .013 25 4C109 54 .012 38 15C34 1 0 22 3C347 338 .002 236 43C200 97 23.4 54 27Subtotal 1174 560 .145 568 125Overall 1642 620 .156 762 154addition to standard two-sample tests that are commonly used for biomarker discovery. An initial evaluation has been carried out by comparing co-methylation networks inferred from healthy and cancer patients, and detecting differential subnetworks. Further experimental evaluation on independent datasets will be required to validate these results. In future work, the methodology could be extended to the case of more than two conditions.Competing interests The authors declare that they have no competing interests. Authors' contributions Algorithms and experiments were designed by Da Ruan (DR), Alastair Young (AY) and Giovanni Montana (GM). Algorithm code was implemented and tested by DR. The manuscript was written by DR, AY, and GM. All three authors read and approved the final manuscript. Authors' information Not Applicable. Acknowledgements This work was partially funded by the EPSRC. Received: 18 February 2015 Accepted: 18 AugustReferences 1. Tusher VG, Tibshirani R, Chu G. Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci. 2001;98(9): 5116. 2. Nacu S, Critchley-Thorne R, Lee P, Holmes S. Gene expression network ?analysis and applications to immunology. Bioinforma. 2007;23(7):850?. 3. Ideker T, Ozier O, Schwikowski B, Siegel AF. Discovering regulatory and signalling circuits in molecular interaction networks. Bioinforma. 2002;18(suppl 1):233?0. 4. Keller A, Backes C, Gerasch A, Kaufmann M, Kohlbacher O, Meese E, et al. A novel algorithm for detecting differentially regulated paths based on gene set enrichment analysis. Bioinforma. 2009;25(21):2787?94. 5. D'haeseleer P, Liang S, Somogyi R. Genetic network inference: from co-expression clustering to reverse engineering. Bioinforma.
Ght to originate from the interstitial cells of Cajal [1]. The management of advanced and
Ght to originate from the interstitial cells of Cajal [1]. The management of advanced and metastatic GIST has considerably improved with the use of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25636517 imatinib mesylate (IM). Approximately 50 to 70 of unselected patients with advanced or metastatic GIST respond to IM and the median progression-free survival (PFS) is 20 to 24 months [1,2] in a highly chemo-resistant disease [3]. IM, originally designed as a specific inhibitor of the Bcr-Abl kinase for the treatment of chronic myelogenous* Correspondence: [email protected] Contributed equally 2 Department of medicine, Centre L n B ard, 28 rue Laennec, 69008 Lyon, France Full list of author information is available at the end of the articleleukaemia, was also shown to be a potent inhibitor of the tyrosine kinase activities of KIT, PDGFR and CSF1R. KIT or PDGFRA mutations are considered an early event in the oncogenesis of GIST [4,5] and are found in roughly 90 of cases. IM is considered a standard of care for patients with advanced disease and as adjuvant therapy for completely resected localised GIST [6-8]; however, its role in the neoadjuvant setting is currently under investigation. The outcome of patients with locally advanced and unresectable GIST is generally considered to be similar to that of patients with metastatic disease. Although first-line treatment with IM produces high rates of disease control in patients with advanced disease, most patients experience disease progression due to the emergence of molecularly resistant clones within 2-3 years?2011 Blesius et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution order Metformin (hydrochloride) License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Blesius et al. BMC Cancer 2011, 11:72 http://www.biomedcentral.com/1471-2407/11/Page 2 ofafter treatment initiation. This observation led several authors to investigate the value of surgical excision of residual disease following response to IM, before the development of secondary resistance. Several publications have reported on the feasibility of surgery following primary treatment with IM [9-14], but little is known about the exact benefit in terms of progressionfree or overall survival. Furthermore, all these studies included patients with both locally advanced and metastatic disease. All were retrospective, except the recently reported RTOG-0132 study [11]. We report here the retrospective analysis of patients with locally advanced non metastatic GIST who received primary medical therapy with IM in the BFR14 prospective trial [15], with special attention to the patients who underwent secondary surgery of their primary tumor.participating site, and no prior surgery, thus excluding patients with recurrent GIST.Statistical analysisMethodsBFR14 populationThe BFR14 trial is a phase III trial randomizing interruption versus continuation of imatinib beyond one year of treatment for non progressive patients [15]. After the results of the randomisation at one year were known, the protocol was amended to allow randomisation after three years of treatment, and more recently after five years of treatment [16]. Inclusion criteria were: age at least 18 years, histological confirmation of locally advanced and/or metastatic GIST, immunohistochemical documentation of c-KIT (CD117) expression, and Eastern Cooperative Oncology Group.
E estrous cycle consists of three major phases: proestrus (high estrogen), estrus (low estrogen), and
E estrous cycle consists of three major phases: proestrus (high estrogen), estrus (low estrogen), and diestrus (low estrogen). The presence of specific cell types is indicative of each stage of the cycle. Specifically, proestrus is defined by having a predominance PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27486068 of round, nucleated epithelial cells, estrus by cornified squamous epithelial cells, and diestrus by leukocytes with few epithelial cells present [30]. Following euthanasia, the hippocampus (including CA1, CA2, CA3, and dentate gyrus) and cortex (somatomotor/ orbital cortices, i.e., frontal cortex) were rapidly dissected. Tissues were then frozen in liquid nitrogen and stored at -80 until analysis. Mice used for immunohistochemical analysis were processed as previously described [31].Mangold et al. Journal of Neuroinflammation (2017) 14:Page 3 ofAnimals were anesthesized with ketamine/xylazine and then transcardially perfused with 1?phosphate-buffered saline (PBS) followed by 4 paraformaldehyde buffered in 0.1 M sodium phosphate buffer (pH 7.4). Brains were then postfixed in 4 paraformaldehyde overnight at 4 , cryoprotected using 30 sucrose, PD173074 web embedded in Tissue-Tek optimal cutting temperature and then frozen in isopentane on dry ice.RNA isolationRNA preparation from hippocampus and cortex was performed according to standard methods [AllPrep DNA/ RNA Mini (Qiagen)] as described previously [32]. RNA quality was assessed by RNA 6000 Nano LabChip with an Agilent 2100 Expert Bioanalyzer (Agilent, Palo Alto, CA). Only samples with RNA integrity numbers greater than 7 were used in subsequent studies. RNA concentration was assessed by relative fluorescence using the RiboGreen assay (Invitrogen, Carlsbad, CA, USA).Microarray analysisTranscriptomic analyses were performed on hippocampal samples derived from male and female young, adult, and old mice (n = 4/group, N = 24) using Illumina Mouse Ref8 microarrays (Illumina, San Diego, CA) according to standard methods and as previously described [5, 33]. First-strand complementary DNA (cDNA) was synthesized from 500 ng input RNA by 2-h incubation at 42 with T7 Oligo(dT) primer, 10?first-strand buffer, dNTPs, RNase inhibitor, and ArrayScript. Second-strand cDNA was synthesized from first-strand cDNA by 2-h incubation at 16 with 10?second-strand buffer, dNTPs, DNA polymerase, and RNase H, purified using the Illumina TotalPrep kit (Ambion, Foster City, CA) according to the manufacturer's protocols and eluted in 19 L 55 nuclease-free water. cRNA was synthesized from second-strand cDNA using the MEGAscript kit (Ambion) and labeled by incubation for 14 h at 37 with T7 10?reaction buffer, T7 Enzyme mix, and Biotin-NTP mix. Following purification with the Illumina TotalPrep RNA Amplification kit (Ambion) according to manufacturer's instructions, cRNA yields were quantitated using a NanoDrop ND1000 spectrometer. Biotinylated cRNA (750 ng) was hybridized by incubating for 20 h at 58 at a rocker speed of 5. After incubation, BeadChips were washed and streptavidin-Cy3 stained, dried by centrifugation at 275 for 4 min, scanned and digitized using a Bead Station Bead Array Reader. Arrays were quality control checked, and initial data analysis using average normalization with background subtraction was performed in GenomeStudio (Illumina). The full microarray dataset has been deposited in the Gene Expression Omnibus, accession# GSE85084. Data was mean normalized and then scaled to make themedian of young males 1 in GeneSpring GX (Agilent).


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Workspace management solution to keep tabs on subscriptions, manage users of your workspace, check-in & exit times and complains lodging
Events broadcasting, posting, and invitation systems for co-working So you can track community impact and keep historical events records
Virtual identification systems to verify subscribers and subscription status
Financial Management tools to receive payments, track subscription rates, track workspace related expenditures, financial reports, and forecastings
Zero Upfront cost to use our solution, and it is always free for you to use
Internal and External Board rooms and Meeting rooms' bookings, payment and management
Internet Access Management, device management, and analytics
Guest management systems to invite, verify and manage guest visitations to subscribers in your workspace locations
Need a specific feature built for you? Let us know. It's at zero cost!

subset: commoncrawl


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Hi Deborah,
A little background on me: I am 25 year old female, athletic (I work out 1-3 times a week, but when training for an event 2-4 times a week), no health issues, im 5'7 and i currently weigh about 170 pounds. My goal and what i am looking for with nutrition coaching is to achieve a healthy weight loss of 20 pounds. My diet is on the go, no fast food but exactly cooked at home or fresh. I am looking forward to hearing about the services you offer and hope they can aide me in my weight loss journey.
Hi Annie,
It is great hearing from you. Would you be able to send me a 3-4 day food log? Time of day is very important...along with any name brands of foods you eat. Once I get the food log...I can access how much time and what kind of session I can offer you...I hope you feel okay with this. It is a great way for me to get to understand where you are at...and where we will go with your nutrition. I look forward to getting the food logs and going from there!!!


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Marvel's Luke Cage Season 2 Review: Bulletproof Crime Drama
Content Editor, Film Critic and Writer for ComiConverse.com, the Founder and co-host of the official Nerdcast Network Podcast
Warning: Season 1 spoilers abound below. If you haven't seen the first 13 episodes, proceed at your own risk.
After a sabotaged experiment leaves him with super strength and unbreakable skin, Luke Cage becomes a fugitive trying to rebuild his life in modern-day Harlem, New York City. But he is soon pulled out of the shadows and must fight a battle for the heart of his city - forcing him to confront a past he had tried to bury.
Luke Cage surprised many with the first season, for its firm tone bringing Marvel's first African-American superhero to streaming services across the country. It moved away from the ninjas, and an ancient evil is seen before, pushing a grounded story in modern-day Harlem. But what is next for the acclaimed Netflix original, after the groundbreaking Black Panther, audiences want more from POC representation.
Following clearing his name in the first season, Luke Cage has now become a celebrity on the streets of Harlem with bulletproof skin and positive outlook. But being famous comes with hurdles, as he needs to protect himself and the city from the evil that lay in shadows. A formidable foe has risen, and Luke is forced to risk it all for Harlem's safety. But will his unbreaking skin, work in his favour? Or does it cause problems for the black communities? All those questions get answered with the 13 episode season of Luke Cage.
Season 2 is the most robust Netflix offering to date, pushing messages about celebrity culture and black issues within modern-day America. It also delivers one of the most intriguing villains in Marvel's television universe, allowing the upbeat Luke Cage to reach another level. There are issues however with loose plot threads and overused extended slow-motion action scenes, but these moments do not affect the structure of the show. I loved the fast-paced approaching to storytelling this time around, making sure audiences aren't left to twiddle their thumbs with padded dialogue. It's also great to see Luke Cage not restricted to an origin story, instead gets to explore the criminal underground of Harlem.
Luke Cage (Mike Colter) the experienced and now famous crime-fighter has returned to Harlem, protecting the city bubbling rivals in the criminal underworld. But, new issues arise with the arrival of Bushmaster who wants to take over Harlem for good and bring in the Jamaican mob. Mike Colter does another solid performance, with the actor's charm filling out the scenery and delivering rich dialogue. I adored the fact the show doesn't feel like a glorified origin story, a problem which always lies in these Netflix shows, instead of further develops Luke Cage a hero for hire.
Misty Knight (Simone Missick) returns as the fiery and intriguing NYPD detective, who is looking to clean up Harlem from the upcoming crime families. The fan favourite's role is more substantial this time around in Season 2, helping out Luke Cage on his wild journey to rid the city from dangers. Simone Misssick is used to significant effect here, balancing the troubles of being disabled in a society filled with questions. These character moments, related to me on another level with her struggles all hitting closer to home.
The villainous John 'Bushmaster' McIver (Mustafa Shakir) is a surprising addition to Season Two, as his Jamaican roots and close quarter combat skills working together in conjunction with Luke Cage (Mike Colter). While his motivations do come across unearned his relentless battle against the hoodie-wearing Cage is impressive and brings tension to scenes, I enjoyed seeing him find ways in breaking the superhero down from the inside. Mustafa Shakir delivers a solid performance, which brings Jamaican culture to the forefront of the 13-episodes, succeeding in following up the great Cottonmouth.
Luke Cage Season Two (2018) is a bulletproof crime drama filled with intense looks at race relations and positive influences in the community, combined with mature themes. It still retains the comic book mentality, with solid musical arguments during intense fight scenes and over the top villainous speeches. It doesn't reinvent the formula but improves in every area when compared to the first season.
Netflix has another hit on their hands, giving Luke Cage a perfect successor to his usurpingly outstanding first season. It ends with a solid villain and engages the audiences with thought-provoking sequences about race. I look forward to more adventures from the bulletproof man and his entourage.
Luke Cage Season Two is on Netflix! Go check it out.


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C Sims Race Report DIRT Series Stop 7
Pala, CA- August 13, 2017- Pala Raceway is one of my favorite places to race. It's close to home, and I love the track! We headed out to Pala on Saturday morning for practice. Everything went perfect during my first practice run. The car was running great and I was able to figure out my lines. I was hitting the double jumps too which I knew would help me during the race. When it was time to start the second practice session, I went back out just to fine tune everything and make sure I had the track down. On my last lap, I decided I would try to clear another double jump that didn't have a smooth landing on the other side. After I hit the jump, my car stayed flat in the air and didn't nose in like it usually does. When I came down, I cased it pretty hard and it knocked the wind out of me. Luckily, my car was fine and nothing broke! I was glad I tried the jump in practice and not during the race! Going into the race on Sunday, I felt really confident in the course and my car.
We had a quick practice session on Sunday morning before racing started. I went out and ran the course again and was feeling great! My class was race number one in the lineup for the day, so after the driver's meeting, I headed back to my pit quickly to get ready. I lined up with the other 8 cars in my class. Pala has a narrow start, and with 9 of us charging it, I knew I had to get out in front fast. The green flag dropped and we took off! I was running neck in neck with one of my competitors to the first turn. We were both determined and neither one of us let off the gas as we headed up the hill and around the first turn. I had the inside line and as we made the turn, my competitor and I hit. I turned sharply to the left, but she flipped on her side. I stayed on it and settled in. I remembered my lines from the day before and was able to keep my lead through the entire race. I finished the first moto in 1st place!
When it was time, we lined up again for moto two. I picked a different spot on the line, but I felt confident I could get to the turn first. As we took off, one of my competitors and I were neck in neck again. Neither one of us let off going up the hill and into the first turn. This time, I had the outside line and I stayed on it. As we headed out of the turn, I was able to get out ahead with the rest of the pack behind me. My competitor stayed with me until we hit the first set of double jumps. I hit the gas and cleared the first set, then I cleared the next set that immediately followed. Clearing those jumps gave me a huge advantage and I gained three car lengths of space between us. I stayed on it throughout the race, and each time hitting those jumps helped me keep a cushion between myself and the others. I finished the second moto in 1st place and took home the 1st place spot overall for the day!
Our team has one weekend off, and then we will head to 333 MX Park in Victorville, CA on August 26th for round 3 of the Quad X & UTV Racing Series. 333 is a track we've never raced at, so we are looking forward to it!
Thanks so much to all of these amazing companies that support my program: Got Sand Performance, Addict Racing Engines, Red Rock, Method Race Wheels, Crow Enterprizes, Factory UTV, GBC Motorsports, RT Pro, Yoshimura, Demon Powersports, Baja Designs, Gates G-Force, Elka Suspension, Rugged Radios, Maxima Racing Oils, Crown Performance Products, Surface Sun Systems, Butter Supply Co, Pavement Recycling Systems, Perrault Motorsports, and my parents who make this possible for me! Thanks to Steve at the DIRT Series for another great day of racing, and thanks to Mad Moose Media for the awesome pictures! You can check out the Sims188 Racing team on Instagram @csims188 or on Facebook at www.facebook.com/csims188/.


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"Tenant" refers to a company or individual that leases space from an owner/landlord. Usually money is paid as rent however, sometimes the landlord will make a trade. For instance, a CPA might lease a single office from a manufacturing company in exchange for doing the landlord's taxes. The space could ... Read More
January 30, 2018Lynn Drake
Rent is defined as "consideration paid for the occupancy and use of real property." This is a general term covering any consideration, not just money.
January 19, 2018Lynn Drake
I often see mistakes businesses make when leasing commercial real estate. Throughout 2018 I'll share with you some things that are easily correctable, and others that have costly consequences. I learned a valuable lesson a few years ago, when I had my furniture vendor make the furniture for our offices. ... Read More
January 17, 2018Lynn Drake
Do You Speak Lease available online through TrueNorthPublishing.US
January 12, 2018Lynn Drake
Meet my dog Rocky. He loves people, but doesn't't like to be referred to as a sheep dog. Because he isn't one. Rocky wants people to know he is a Aussie-doodle. What does that mean? His mother was a standard Poodle and his father was an Australian Shepard. He believes ... Read More
January 11, 2018Lynn Drake
I was a terrible math student. How and why I ever decided to get a degree in accounting is a mystery to me! One of the first jobs I had after graduation was working for a plant that sold steel. My job, (I can't believe I ever did this!) was ... Read More
January 9, 2018Lynn Drake


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This was a party to celebrate a 70th birthday that had to combine understated elegance and a unique, fun filled evening that got all the guests involved.
The party took place in a large free standing marquee on the front lawn overlooking the wonderful view of our client's stunning home. As the birthday party was in the Spring it became dark early therefore we had the fantastic opportunity to light various trees and sculptures in and around the garden and driveway to add atmosphere and create a sense of fun right from the outset.
Guests were seated at intimate square tables dressed with elegant linens and tableware in subtle golds and natural shades. Each table also had a lamp with a bespoke shade detailing an array of fun family photos. A delicious 3 course dinner was served whilst the guests were entertained by some very fun acts including a mind reader, Tenors Unlimited and even the host himself who treated the guests to a choreographed dance!
The evening's finale was a Ceilidh band – it certainly was a fun packed evening for all.
"You should feel proud of the amazing event you pulled off for Crispin on his big day. Your creativity and attention to detail was incredible.
Plus – you never stopped and stayed focused from beginning to end, even there on event day directing cars and there at crack of dawn to oversee the clean-up.
You are quite unique."


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Joyous SamhainPosted 2012.10.31 11.51 in Spiritual by
Blessed be, everyone.
You are currently browsing the October, 2012 archives.
Blessed be, everyone.
I could be wrong, but I'm pretty sure that as of this morning, I've finished the electronics on the Integrated Sensors Electronic Bracer Mark III. (Wait haven't I said that before?)
I've mounted the last electronic component. It seems to be working fine. All that remains to be done from this point, is finishing the software and the new leather bracer. And an enclosure.
The last part was the micro TV-B-Gone that I assembled a while back.
I'm pretty happy with how things have turned out, even if it has taken a few months to get to this point. It's all self-contained and even though there are a couple wires, nothing is going to be flexing around – it's one solid unit.
One minor challenge is the name – ISEB-6 is not entirely applicable as the Mark III has more than 6 sensors… and ISEB-8 just doesn't roll off the tongue as well. #madscienceproblems
"Holy bleep, how did it get so late? Like blink and the last eleven hours just zipped past!"
The ISEB-6 Mark III revision D is alive! I put the finishing touches on the soldering this morning. All that's left now is finalizing the software. And making the actual leather bracer. And mounting the electronics. And building a slick enclosure. And…
Ok there's a lot more to go yet. But still – look!
Isn't it kewl?
The only real bother I ran into was the display's only got 3 bolts instead of 4. It's still sturdy, the data connections are all soldered solid. The problem was when I routed all the traces, I forgot to leave room on both sides of the board for the hardware. So the top-left corner under the screen has two SMD resistors that would short against the nut, and the bottom of the board has two traces that would be cut if I counter-sunk the hole to accomodate the flat-head bolt. Bummer.
Apart from the problem with that one bolt, everything else went together fairly smoothly. Mostly.
The image below shows it almost ready for the screen – the BMP085 is in place for sensing temperature and air pressure; the ADXL345 is in place for measuring accelleration. I've also added a white LED to serve as a flashlight, and with some creative snipping and soldering, I fit the Lillypad Vibration motor in underneath the screen too!
When the whole thing's done and finished, I'll be posting everything (code, board layout, BOM, assembly) so anyone can make their own ISEB-6.
Also noteworthy: there are still two available analog inputs (A0 and A1) and three available digital IO ports – B2, C2, and C3. Expansion possibilities!
I positioned C3 near the ICSP port so one could add a SPI peripheral by using C3 as a slave select (the display is already using the default SS pin). C2 is on the sub-board with the buttons, to allow expansion there. The other three available I/O pins, along with the I2C bus and power are available at the top edge of the board for easy expansion.
Last night I started the build of the latest version of the ISEB-6 circuit board. So far it's all been surface-mount parts, but I was able to get it to this point using a standard handheld soldering iron with a fine tip.
The smallest-pitch part is the FT232R chip (USB to serial) which has 28 leads that are only 3/10ths of a millimeter across, and about 3/10ths of a millimeter between each pin. That's basically soldering with your nose to the board, the soldering iron right next to your face, and while using a 5x jeweller's loupe to see what you're doing.
At least, that's my technique.
Apart from the microcontroller and the USB chip, I've installed & tested the GPS module, temperature sensor, humidity sensor, light sensor, a voltage-divider for monitoring the battery charge, the voltage regulator and the LiPo charger. And of course all the sundry supporting passive components, and a smattering of LEDs.
There's only about a half dozen surface-mount parts left to be mounted. The three remaining ICs though are tricky. They don't have leads – they have pads on the bottom, so you can't actually get to them with a soldering iron.
To get these soldered, I need to use a hot air tool and some luck. There's the air-pressure sensor, an accellerometer, and the magnetometer. I'll put them down in that order (from least-complex to most, and least-expensive to most.) and test between each one to ensure they're working. All three use I2C so it's pretty straightforward to test if they're working or not.
Then the last bit of work on the circuit-board is to mount the display screen. This will require a little bit of hardware work to securely mount it with the mounting holes. It has to be last since it covers some of the other circuitry.
And after that, the leather-work begins – making a new wristband, and mounting / enclosing the circuitboard.


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Privately held candy maker, Mars confirmed that it is buying Wrigley's for $23B in partnership with Warren Buffett. The combination would match up confection brands like M&Ms and Snickers with Orbit, Extra, Altoids and Lifesavers. The deal would rank as the third biggest announced deal of the year.
Here are the top deals of 2008 to date (Thomson Reuters):
Note: The Novartis / Alcon deal is a two-stage deal. Total value is over $38 billion.


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These media sources are slightly to moderately conservative in bias. They often publish factual information that utilizes loaded words (wording that attempts to influence an audience by using appeal to emotion or stereotypes) to favor conservative causes. These sources are generally trustworthy for information, but may require further investigation. See all Right-Center sources.
- Overall, we rate the Daily Mining Gazette slightly Right-Center biased based on ownership affiliation and editorial positions that slightly lean conservative. We also rate them High for factual reporting due to proper sourcing and a clean fact check record.


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John Gilbert "Jack" Pascuzzo, age 88, of York, died Wednesday, February 20, 2019 at his residence.
He is survived by his wife, Shirley Kellogg Pascuzzo , his son, Chris (Sylva Palmer) Pascuzzo of Retsof, his daughter, Cheryl (Joel) Shoemaker of Niagara Falls, grandchildren, Amie (Chad) Boggs, Kara (Ben) Nowak, William, Maxwell and Kathryn Shoemaker, great grandsons, Cody Boggs and Clive Nowak, many nieces and nephews.
John was born March 7, 1930 in York, NY the son of Thomas and Marion Gilbert Pascuzzo. He was a graduate of York Central School where he retired from in 1992 and was one of the first classes inducted into the schools Hall of Fame. Jack was a former member of the York Town Board, officer for the Retsof Hose Company and served the Ambulance Service for 15 years. He was also a Trustee at the Geneseo United Methodist Church. Jack loved to travel visiting many countries, National and State Parks including his favorite, Adirondack State Park. He also enjoyed sports, especially bowling and talking Politics.
New listing on Conesus Lake!
Calling hours will be held on Sunday, February 24, 2019 from 1-4 PM at the Rector-Hicks Funeral Home, 111 Main St. in Geneseo. Funeral Services will be held on Monday, February 25, 2019 at 11 AM at the United Methodist Church in Geneseo with Rev. Keith Griswold officiating. Burial will be in Pleasant Valley Cemetery in York.
Memorials may be made to the York Ambulance Fund, P.O. Box 95, York, NY 14592 or the Geneseo United Methodist Church, 4520 Genesee St. (Route 63), Geneseo, NY 14454.


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The clue "Thespian org." was last spotted by us at the New York Times Crossword on October 1 2020.
Featuring some of the most popular crossword puzzles, XWordSolver.com uses the knowledge of experts in history, anthropology, and science combined to provide you solutions when you cannot seem to guess the word.
Keep in mind that we have over 3 million clues with their own respective solutions so feel free to search on the search bar on the top of the page if you think that this is not what you are looking for.
We found 1 possible solution for the Thespian org. clue.
Do you think that the solution proposed for this clue is wrong? Head over to the comments section and tell us what do you think is right!
Want to know where AFTRA has appeared as a solution before? Click here for more information on that word.
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|Grand in scale
|Potentially offensive
|Let the air out of
|Prefix meaning one-billionth
|___ Lum, a.k.a. actress/comedian Awkwafina
|Go deep
|Stage a coup
|Meat scraps
|"The way"
|Titular "married lady" in a "Funny Girl" song
|Burns a bit
|Pick a spread, say
|Emma Stone's character in "La La Land"
|Word after Scotch or duck
|Sport in which rikishi wear mawashi
|Fearsome beasts
|Products since the 1960s ... on which you can see five "key" things depicted in this puzzle
|Wire holder
|"Law & Order: SVU" actor
|Spot for a napkin
|Voting no
|Coral island nation north of Fiji
|Oft-repeated slogan
|Cooped up like pigs
|Coupon clipper, e.g.
|Genre of "The Big Sleep" and "Kiss Me Deadly"
|Fifth grader, often
|Mineral in some geodes
|Waterfall sound
|Thick and clumpy
|First name of a famous Mongol ruler
|"Twittering Machine" artist
|Grade school subj.
|Course objective
|Playground "germ"
|"For ___ whomsoever much is given ...": Luke 12:48
|"Me neither"
|Athletes first allowed in the Olympics in 1988
|Band with the 1970 #1 hit "Mama Told Me (Not to Come)"
|French daily founded in 1826
|Oscar nominee Woodard
|Some paid spots
|Fish whose blood is toxic to humans
|"Uh-uh!," in Edinburgh
|"Never mind, it's fine"
|City of the Peloponnese
|Discovered, as a shady past
|"Throw it here!"
|The usual suspect
|Mopey and ultra-introspective
|Vodka mixer
|Substitutes for feet
|Zhou in Chinese history
|Co-founder of the Black Panthers
|Classic checker-dropping game
|Green prefix
|Cassette tape predecessor
|Aunt ___, despised relative of Harry Potter
|Like rails and stilts
|Thespian org.
|Covetousness, for one
|___ beetle
|Shout of victory
|Material in automobile batteries
|Dr. J's team
|Many a hoedown participant
|Scrape (by)
|Manhattan ingredient


text: (Beaver Dam) A Sun Prairie woman was sentenced to three years in prison Tuesday for selling drugs in Beaver Dam. Roxanne Sobrevilla pled guilty to one felony count of Manufacturing and Delivering Heroin in December. A confidential informant told a detective from the Wisconsin Department of Justice that he could purchase heroin from Sobrevilla, and did between 2013 and 2014. The informant contacted Sobrevilla who agreed to sell a gram of heroin to him. The 30-year-old said she would travel to Beaver Dam to meet him. The two met in a parking lot and Sobrevilla sold the informant four grams of heroin for $600. District Attorney Kurt Klomberg said during sentencing that Sobrevilla has been living a criminal lifestyle for nearly two decades and has participated in the wholesale delivery of heroin in Dodge County. In addition to prison, Sobrevilla will be placed on extended supervision for four years.


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How to Mirror Flip Your Screen
Need to mirror flip your screen? There's a very simple solution to that: we'll show you exactly how to do that in this in our step-by-step guide. You'll be able to flip your Windows PC, Mac, iOS or Android device.
In addition, we'll show you how we used a mirror flipped display in our studio to create fast videos and deliver speeches.
Keep reading to find out exactly what you'll need:
There's a secret service menu Samsung doesn't want you to know about. Once you access this menu, you can easily flip your screen.
How To Use Mirror Flipping
We use the mirror flip monitor in our own studio to create fast, pro level videos. Check out our teleprompter training lessons using this technique.
We use the mirror flipped display every day in our studio for video production. I'll take you through all my best tips and tricks for how to flip your screen easily and affordably.
More Mirror Flipping Techniques
1. Use a chrome plugin such as "flip a video for youtube" or "Mirror Page" https://chrome.google.com/webstore/detail/mirror-page/ncjklfgaapegkmjkkcgpognokjnkmdfa?hl=en
2. Use hardware such as an HDMI MirrorBox. It can automatically flip your HDMI signal.
3. UltraMon software supports mirror flipping. https://www.realtimesoft.com/ultramon/
4. Flip it by bouncing it off another mirror first. Specifically, a first surface mirror so you don't get a double reflection (also known as "ghosting"). Just angle it between your display and the beamsplitter glass.
5. Use teleprompter software that has the feature built-in
6. Insert a simple line of code into an HTML document.
7. Use Snagit to screen capture an entire document at once, then mirror flip it in photoshop or another photo editor.
8. When mirror flipping an iPad, simply use the Apple USB Type-C Digital AV Multiport Adapter so you can display your iPad on a TV.
Do you have any other mirror flipping techniques we may have missed? Let us know about it in the comments below or contact us!
Our Other Products
Presidential Model
Check out our Presidential Teleprompter, our best-selling model.
Teleprompter Mirror
Buy the optical beamsplitter by itself for your project.
Buy Sample
Order a sample of our optical quality glass without breaking the bank.


text:
~ Crochet Hat Pattern ~
This hat pattern is easy, yet stylish enough for anyone to wear. It is crocheted in a self striping yarn to give it a nice blend of colors without having to do any color changes.
You will need a worsted weight yarn, an I crochet hook and two buttons.
If you enjoyed this free crochet pattern you might also enjoy more designs by Beatrice Ryan Designs.


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Young Generations and Embracing the Digital World Will Dictate the Changes in Post-Covid Consumerism
May 2020
DIRECT MEDIA United Solutions
Conclusions of the webinar DIRECT TALKS From Home: Insights vs. Myths: What really awaits us after Covid19?
Whether they were created before or after the coronavirus pandemic, brands will settle their problems in a new communication with younger generations. Virtual events have become must in strategies. Not all influencers have the same prospects for survival. The gaming industry is big-time mainstream. The users for large screens are finally discovering their functionalities – these are the conclusions of the 7th edition of DIRECT TALKS From Home webinar, with the participation of:
Sanja Dimitrijević, Group Account Director, We Are Social, Germany
Katarina Todorović, Product Owner, United Cloud
Jelena Stošić, Strategy Director Kids Industries, The Family Agency, London, UK
Miloš Skokić, Managing Partner, Žiška
Discussions moderated by: Ljubica Vukčević, Head of Research & Insight, DIRECT MEDIA United Solutions
The desire of youth to belong
From the very first day of the lockdown in the UK, the Family Agency from London has followed the changes in the habits of families, which changes indicate that we are on the verge of more lasting changes of behavior with that target group of consumers. According to Jelena Stošić, mental health has gained in importance. Teenagers report anxiety at a 13% greater rate than before and parents and children attend to such issues together. "It's sad to see that a 10-year old child is worried because their father has lost his job and the majority of the population is concerned whether they will have enough to eat, which is quite a new and unusual concern for England". Children for the most part miss school as an opportunity to move around freely and socialize with others. Furthermore, one of the main trends is the acceptance of the digital world by the parents. As yet another proof that the world has moved towards the online realm is Travis Scott's concert in Fortnight, which was seen live by 12 million people. Until then, the most visited concert was that of Paul Mccartney for 3.5 million people", Jelena says. In her words, these changes open new opportunities – ones in the field of communications and others in area of product features. "The communication aspect must be carefully managed and as for product development, this generation will be marked by a desire to belong and seek out for their respective communities. All this has to be identified and properly used by the brands," Jelena indicates.
The virtual world as a must
According to Sanja Dimitrijević, the German-based agency We are Social stepped deeply into the world of virtual events during the pandemic, believing that such events will become an integral part of 360 strategies. "We had an experience with our clients from Audi, when the Geneva Motor Show was cancelled 36 hours before the launch of the new A3 model. We did a live stream for the first time and it all went brilliantly. The outcome is that even much more conservative clients have now realized that it's not such a big deal anymore, but perhaps the only way to organize events in 2020 in a completely legitimate way," Sanja says. She points out that virtual events can be incredibly complex and that it is important to include social networks for support, in order for interaction to happen and people to feel engaged. "Those clients that are not willing to risk with stream opt for microsites with pre-prepared content; they are also considering AR and VR and while nothing can replace human touch, I am sure that strategies will include at least one or two virtual events per year. First and foremost for the financial savings they generate, as well as the more accurate targeting, simplified follow-up, etc." Sandra says.
New habits for larger screens
The number of subscribers on streaming platforms skyrocketed during the pandemic, but it was already up nonetheless. According to Katarina Todorović from United Cloud, the coronavirus situation has proven to be a golden age for streaming platforms that gained in popularity. "The ratings of streaming platforms at weekly level increased by 400 years and by the end of 2020, these platforms are expected to gain an additional 45 million users. In Serbia, SBB unlocked its content at the very start of the pandemic; content ratings have increased, as has the number of competing streams, namely the number of users watching simultaneously a specific content, by almost 50%. As for the type of content, TV remained at the same level but VOD content, series and films are up 30%, just like radio on our EON platform, which is a good indicator of people becoming aware of all the functionalities of the platform. New channels have grown as expected, children's channels remained the same, sports channels have gone south, entertainment is up and adult content has tripled. The number of devices used at household level increased – three devices in average are used, which is good", Katarina says. In her words, the use of mobile phones during the pandemic is down, which indicates that the smart phone is a "fast device" in terms of consumption, as evidenced by the fact that only news content watched on mobile phones is up, while the use of larger displays has increased significantly. "While the ratings have fallen after the state of emergency was lifted, the good news is that the consumers have become aware of all the functionalities of the platforms and are expected to retain certain of these newly acquired habits going forward," Katarina says.
Influencers will survive
When it comes to influencers, according to Miloš Skokić from the Žiška agency, there will be no full return to the old situation and the recession will most definitely result in a decrease of consumption for this channel. "As for the influence and role of influencers during the pandemic, it was observed that content creators survived, as opposed to influencers in the narrow sense, who were focused on self-promotion. We also have cases of high-quality content creators who were marginalized due to the inability to use physical space in terms of content production. In Serbia, the perception is that the influencers are the icing on the cake and if the brand managers are put in the position to have to renounce something, I think it will be them. The value of that market will go south, but only temporarily. The end of 2020 and beginning of 2021 will be more marked by an ad-hoc, timid collaboration with influencers," Miloš says. He stresses that the global trend related to influencers and the bureaucratization of the process of cooperation via intermediaries or agents exists here too, which is, in his words, a good thing, since it opens up the possibility for better managing that cooperation.
"The value of content means a great deal. What we see as a trend with children is that the main influencers, which are from three main areas – gaming, toys unboxing and challenges – are relatively resilient categories. They switched to TikTok and that is super fun for children. Generally speaking, influencers here are more resilient to such changes, since it is officially a well-organized industry and they have become the standard for campaign planning", says Jelena Stošić. She indicates that the most desirable job for children 7-11 has until recently been the influencer. This changed in the last month, when the top of the list was overtaken by the doctor, while interest in medicine and science increased. "A very interesting example is the toy brand Matteo, which identified the new trend and quickly launched a line of toys representing postmen, doctors, personnel helping in the pandemic… In normal conditions, this process lasts in average 12-18 months from the emergence of the idea to the realization thereof.
In Germany, the influencers are on the rocks, since their ratings, according to Sanja Dimitrijević, have plummeted due to the declining trust of their audience. "With this new phase in people's lives, the perception of influencers is even more black and white. However, we have also noticed that last year, user-generated content suffered a serious setback, only to jump by 24% now that people stay at home. Creativity is valued and that is why people love TikTok and anything that challenges them, without requiring major investments of time and money," Sanja says.
Gaming is big-time mainstream
According to Miloš Skokić, in terms of gaming we will see a short-term growth, followed by a mild decline mid-term and renewed growth on the long run. "Let me first explain the distinction between gaming and Esports. Gaming includes any type of mobile games, from Candy Crush to serious console games, while Esports is professional and competitive gaming such as Counterstrike, League of Legends, Fortnight… While gaming is on the rise, streaming and the time spent gaming are up, the number of Esports events is down, since tournaments involving actual team competitions have been cancelled. What is also a good thing is that most Esports leagues continued to function," Miloš says. In his words, while gaming has been a niche for a long time now, it has become mainstream big time. "The biggest brands are now sponsoring Esports competitions, such as Pepsi Cola and Coca Cola, while BMW is the main sponsor of each of the best teams in places where Esports thrive, such as the US, Korea, Europe and China. This is a precedent in terms of sponsoring, because you also have Gillette, as well as brands from the sector of banking, insurance and savings that sponsor various segments in the live transmissions and create relevant content for the users on this platform," Miloš says.
New values
According to the participants of the webinar, values will be the most affected by the changes and accordingly the perception of certain products, services and brands.
"When we talk to 10 or 11-year olds, we can see that things are changing dramatically. Young people have different values, starting from the respect for life resources and changed perception about the actual possession of products. The entertainment industry, which profits from the sale of products, are having a hard time creating love for the brand in times when everything is short-lived and there is no long-term memory. What's lacking is something that will generate love for the brand and the content", Jelena Stošić says.
According to Sanja Dimitrijević, premium brands in the car industry will survive only because they have recognized on time the problems that emerged before the coronavirus pandemic – namely that the younger generations prefer car sharing to owning a car, which used to be the Holy Grail. "Turning to sustainability is the path many brands will take", Sanja says.
"The narrative is changing. Games are becoming an important social mechanism, as evidenced by the popular virtual concerts and cooperative games. We can expect more games in the most general sense, in the virtual space, where we can socialize, hang out and discover aspects of our personality that we don't see in the online world", Miloš Skoric concludes.
Watch the entire webinar:


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Former academic, traveler, Third Culture Kid.
Writing about academia, travel, culture, and everything in between.
10 June 2019
Book Review: The White Castle by Orhan Pamuk
Anyway, this novel is about a travelling sailor in the 17th century, coming from Venice, who has been captured by the Ottomans and taken as a slave in Istanbul. He becomes the property of a man named Hoja, who seems to bear an uncanny resemblance to him. They have a master-slave relationship, but sometimes in the course of the book, this relationship flips back and forth. At some time, the reader is now not sure who is the master and who is the slave, since both look very similar to each other, and both know a lot about the other, so each can inhabit the other's personality and pretend to be the other one. And in fact, at the end of the book, somehow the narrator flips, indicating a very unreliable narrator, since in the beginning, the book was narrated by the slave, and now it seems that the book is ending with a narration by the master. The reader therefore never knows if the two characters indeed have exchanged personalities, or not.
I must say however that this book was rather easy to follow, and I wasn't lost at all in the historical aspects of it. But there was still something that I felt was missing. It lacked a certain oomph perhaps, and in the end, I felt like it didn't have enough negative aspects for me to hate it, but at the same time it didn't have enough positive aspects for me to like it either. I felt very ambivalent about it in the end. And I was only glad to have finished it because at some point I would like to read all of the novels that Orhan Pamuk has published.
I am aware that there are several themes running in the book, and if you read the corresponding Wikipedia article for the book, you will learn what they are. I just felt like it wasn't made prominent enough in the book, or if it didn't it appeared as if it wasn't too noteworthy to focus on. My most charitable takeaway for the book was that it was historical fiction, and that I learned a little about 17th century Ottoman Empire. But beyond that, I am afraid that this isn't the most memorable book for me.
I give it 3 out of 5 stars.
See my other book reviews here.
Categories: Book Review
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Cleveland HiBore XLS Fairway Wood Review
Last Updated:
What we say...
The fairway woods are similarly forgiving and with heel and toe "stability foils" like the rest of the range it means they are also very stable and easy to align at address.
And talk about big.
The new Cleveland Hibore XLS fairway woods are an incredible 24% larger than the Hibore XL golf woods and for game improvers that can mean only one thing. They are much easier to use.
Better players may be put off by the traditional driver sized three wood but anyone looking for extra forgiveness will love the way they catapult the golf ball into the air, irrespective of where the golf ball connects with the oversized face.
Contact: 01252 761300
Web: www.clevelandgolf.com


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I have a Samsung Galaxy S7 with FRP-locked, USB Debug is off and I am trying to disable FRP and enable USB Debug. Is there any way to do this with Odin or Heimdall?
I also have the stock ROMs for the phone, and no, the previous owner does not remember the gmail account she used for the phone. The Model number is G930P, and the carrier is Boost Mobile (Sprint, USA).


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Question: Just wondering, do you have any spoilers for NBC's Las Vegas?
Answer: There's a really fun episode coming up in which Vanessa Marcil's Sam has to play host to a high roller afflicted with a bad case of sweaty palm syndrome (aka palmar hyperhidrosis). After the guests start complaining, Ed pressures Sam to either come up with a remedy or kick him the hell out. Meanwhile, Danny is dealing with a PR headache of his own when a hotel guest wakes up missing a kidney. Nocturnal kidney theft is apparently a classic Vegas urban legend. Did not know that.


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Lauderdale County is very blessed to have a wonderful and caring Superintendent, Assistant Superintendent, and Director of Special Programs. Our question is are our children with disabilities receiving the treatment they should be receiving in our schools? Sometiimes they create problems by making wrong choices, which they have to receive punishment, but should they be intimidated and be put in situations where they are pushed to make more bad choices? We are wondering if there are other parents/grandparents and guardians who might be asking these same questions?
Thank you,
Kenneth and Chestene Gressett


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Racer X Films: Shadowing Doc G at the Races, Part I
May 1, 2018 3:20pm | by: Kyle Scott
Doc G is a full-time chiropractor in addition to traveling to the races on the weekends to "tune-up" the riders. He's worked with just about every top rider in the past 20 years beginning with Tim Ferry and Ezra Lusk.
Doc G's current patients at the races include: Marvin Musquin, Cole Seely, Cooper Webb, Christian Craig, Justin Bogle, Broc Tickle, Aaron Plessinger, Austin Forkner, and more.
We shadowed him for a day at the races and it was as educational as it was entertaining.
You can follow him on Instagram at @teamdrg.


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Fri, 13 July 2018
With voice search becoming more and more prevalent these days, is it only a matter of time before it bleeds over into ecommerce and becomes the way people shop in the future? Joining me today to discuss this possibility are Miracle Wanzo of HipUndies.com and Bill D'Alessandro of ElementsBrands.com. We each share our predictions for how voice search will impact the future of ecommerce, as well as possibilities for how this technology could evolve moving forward.
You can find show notes and more information by clicking here: http://bit.ly/2MAVTXI
Direct download: EP230ECOMMERCEFUEL.mp3
Category:general -- posted at: 6:00am EDT


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Home Tags History
Tag: history
IHRC Bookshop Updates
IHRC Bookshop UpdatesIHRC Bookshop & Gallery have a few announcements to share.Book...
History Made June 20th in San Francisco–Dockworkers Refuse to Cross Picket...
Message from the Palestinian General Federation of Trade Unions (PGFTU)
The Universal Theology of Liberation: Views from Muslim history
Talk and Q&A with Dr. Mohamad Nasrin Nasir and Dr. Daud Abdullah.With a set by performance poet 'Truthseeker'
Event Alert: UK – The universal theology of liberation – Views...
IHRC event: "The universal theology of liberation – Views from Muslim history". Speakers include: Dr Mohammad Nasrin Nasir, Dr Dawud Abdullah.
Press Release: Torture by British troops in Iraq typical of...
IHRC condemns in the strongest terms the abuse and torture of Iraqi prisoners by British occupation troops.
A Brief History of Palestine
Shelina Janmohammed gives a short chronicle of Palestinian aspiration and nationhood, and the atrocities committed against it.


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Georgia Tech coach Paul Johnson's comments following the Yellow Jackets' 38-28 win over North Carolina.
Opening statement:
"It got a little crazy there. I thought maybe when we came out and opened the third quarter and put together a nice, long drive for a touchdown and then we got 'em pinned back inside the 20 on the kickoff that maybe we'd get away from them a little bit. But to their credit, they kept playing and they fought back and we did most everything we could to try to help them. Two fumbles when we were going down the field and the fourth-down play, we bobble the snap. I was watching when we were running the play, we had it sealed pretty good; I thought it was going to be a pretty big play and I look and we dropped the snap. I thought the defense did a great job there at the end getting a couple of turnovers when we had to have 'em and we did what we had to do to get out of there winning the game."
On three lost fumbles:
"The fumbles are frustrating. And we've got to do a better job coaching that. They're coming from the same position. We've got to do a better job coaching that."
On play of B-backs:
"We need to be able to finish those runs. Those are the ones that KirVonte (Benson) could finish a year ago. They're capable. My thing with our guys, too, is, and this comes back to coaching as well, we have to be more aware of the situations. When you come up this short of the first down on third down because you're trying to break one, you've got to get your pads turned and get your pads turned and get the first down. It came back to bite us in the first half."
On Brad Morgan playing right guard in place of Connor Hansen:
"It didn't matter. They beat the fool out of us in the tackle box. They beat the fool out of us in there. You can say what you want. We rushed for 460 yards. Everybody talk about what a great job they do. Keep telling them that. They got their (butt) whupped."
On playing TaQuon Marshall and Tobias Oliver:
"I went into the game, and I said this morning in the meeting, we'll play 'em until they don't score. And really, we weren't scoring, but we weren't punting either. We were driving the ball and then taking it down the field, and we'd turn it over. At the time, this game, I felt like that with what we were running, (Oliver) gave us the best chance. The next game might be the other guy. TaQuon went in and led us on a touchdown drive to start with. He didn't really do anything wrong. It was a gut thing, a feel thing. Probably the rest of the way, that's kind of what it'll be."
On cornerback play:
"We don't play the ball very well. The reverse pass they threw, we're right there. (Lamont Simmons) probably got pushed, but we were right there; we don't make a play. There were two or three balls where they throw it, we're right there. We just don't play the ball very well."
On winning:
"Anytime you can win. I told somebody before the game, this team has some talent. They're just kind of snakebit. I told Larry (Fedora) before the game, they're probably not any different than anybody in our division in fact, they may be as talented or more talented than some. but sometimes, we've been there where you find a way to lose. We tried to today where you find a way to lose the game and that's just kind of been what's happened to them. But they've got some talented kids, especially skill kids offensively."
On second-guessing going for it on fourth down in the second quarter:
"When I didn't make it, I was. We've been pretty good on fourth-and-ones and fourth-and-twos. My thought process was, it's 21-10. If we can get this in the end zone and go up 28-10, we get the ball first in the third quarter and go up 35-10. Then we don't have to worry about a whole lot; you've kind of got the game in order. And we didn't make it. All the other fourth downs, I think we did make except the one we dropped the snap. It's just like at the end. You can second guess yourself, but you could also have kicked the field goal on fourth-and-2 when we went on in and scored the touchdown to go ahead. I don't think anybody would have felt real comfortable over there up three."
More from Chapel Hill:
Backup Tobias Oliver gets in the game and goes the distance


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One would have thought that the Nigeria movie industry would help the masses see some professions in positive light. But it is disheartening how they have been poisoning people's mind and tarnishes nursing image in Nigeria.
(You want to know why?, keep reading).
Oh! my dear Nursing, see what Nollywood has done to you.
Am a Registered Nurse – not a Physician, not a police officer – so am going to be talking about Nursing and Nollywood through this article.
How Nollywood See Nursing
As a Registered Nurse and an avid observer, Nollywood and it's 'practitioners' present Nursing and Nurses in purely negative light.
Probably they do not have a clue of what we are and what we do.
Nollywood sees Nursing and Nurses as;
1). Below-average professionals
Nurses are not below-average in any way. We attend Universities, Schools of Nursing like other professionals.
2). They see Nurses as those folks in white uniforms claiming to be a nurse after spending some time in private hospitals, medicine shops to be trained as one.
Those people trained in private hospitals and medicines shops etc. and have not been to either University or School of Nursing to study Nursing as a science and an art and are duly registered by Nursing and Midwifery Council of Nigeria (NMCN), ARE NOT NURSES. If am not going to wreck psychological havoc, THEY ARE SIMPLY QUACKS.
But people with high level of ignorance refer to them as Auxiliary Nurses. Since they create a negative mental picture in their psyche about Nursing and Nurses, they employ the service and ways of the quacks to portray to the public what Registered Nurses are.
3). Nollywood portrays registered Nurses as prostitute that sleep with Doctors and patients while on duty. As a matter of fact, Registered Nurses are not prostitutes. The quacks may be.
YouTube is full of such movies where Nurses are portryaed in this way. What people see is what they believe.
Recently I stumbled on a blog post, TheInfong, but because the writer of that blog post is not a Nurse, he thinks that is what Nurses do. He said, See what Nurses do with their patients in the hospitals at night. So nauseating.
4). Nollywood portrays Nursing as a profession that answers YES! to other health professionals without questioning. No, we are not!
As our code of conducts have it, we only stand up to greet our senior colleagues while in uniform. We do not do that for other professionals since almost every household in this country has access to movies, series etc and because they tend to believe what they see, they believe what Nollywood shows and thinks about Nursing and Nurses in Nigeria is really what Nursing and Nurses are. No, we aren't the Nollywood stereotyped Nurse!
What Nurses Really Are
To simply put, a Nurse is an individual who went to an accredited University or School of Nursing to study the science and art of Nursing, registered with NMCN to be able to render timely medical help to the sick in collaboration with other health care professionals.
- Nurses respect patients' privacy.
- Nurses know patients' bill of right.
- Nurses have rationale for whatever they do.
- Nurses have code of conducts guiding their conducts.
- We have regulatory body.
Registered Nurses are not some folks in white uniforms, gossiping, sleeping with patients, doctors that Nollywood thinks we are.
What Nollywood Can Do
To me, it seems Nollywood do not know a thing when it comes to the roles Nurses take in the hospital, if you are going to make a movie about Nursing and Nurses, make it well. What is worth doing at all is what doing well.
Nollywood should take their time to understand how some professions perform their duties before going out to make movies that will dent their images and poison people's mind.
Someone once told me, those things Nollywood shows about you is what you do.
To learn more about how/what registered nurses do and how they do it, the likes of Nurses On Air, Nursing World Nigeria, Fellow Nurses Africa etc. are more than eager to help and give the proper orientation.
What Nurses Can Do
Are you a Registered Nurse that loves acting? Are you fed up with the way Nursing is being portrayed by Nollywood in movies?
I charge you to dig your way into the movie industry and infect them with the right images of what Nursing and Nurses really are!
Obembe S.D, RN
(AssistBlogger & Daily Health Nigeria Boss)
Do not leave this article without sharing with someone. Nursing is great already!👍


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