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Can anti-GFP detect YFP?
Q: What GFP variants will this antibody recognize? A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.
Does GFP antibody recognize EGFP?
GFP antibody (ab6556) is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.
What is the difference between YFP and GFP?
GFP stands for Green Fluorescent Protein, which was originally derived from the jellyfish Aequorea Victoria. YFP stands for Yellow Fluorescent Protein. GFP exhibits an excitation peak at 395nm wavelengths,s and its emission peak is 509nm wavelengths. YFP emission peak is 527nm and excitation peak is 515 nm.
Can YFP and GFP be used together?
Concurrent use of GFP and YFP could be highly advantageous in many cellular assays, particularly when using cells of transgenic mice whose cells express fluorescent proteins, because generating mice with alternative fluorescent proteins can take a lot of time and effort.
What is anti GFP?
Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.
Does GFP antibody recognize mCherry?
In most cases GFP and mCherry antibodies do not cross react, but you will need to check for yourself especially in the manufacturer does not mention it. These two antibodies do not cross react with the other fluorescent protein.
What wavelength does YFP absorb?
In the Nikon YFP HYQ block, excitation is limited to a 20-nanometer band of wavelengths in the blue-green spectral region (490 to 510 nanometers), and is coupled to an emission filter transmitting yellowish-green fluorescence in the range of 520 to 550 nanometers.
Can I use CFP and GFP?
"" Using CFP and GFP together can be challenging, but it is feasible. The CFP emission curve overlaps significantly with the GFP curve. However there is no significant excitation of CFP by the blue laser, so you should be OK in this regard.
Why is GFP fluorescent?
GFP is a barrel shape with the fluorescent portion (the chromophore) made up of just three amino acids. When this chromophore absorbs blue light, it emits green fluorescence.
How big is GFP?
GFP is big GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence and therefore GFP cannot be downsized by deleting residues.
What is GFP and RFP?
Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP) are ~29 kDa fluorescent proteins used as versatile markers for visualization of protein localization, monitoring physiological processes, and for detecting transgenic or transient protein expression.
Are there any antibodies that are reactive against GFP?
This antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP. Recombinant full length protein corresponding to GFP. Pure GFP protein, or cells known to overexpress GFP can be used as a positive control in WB.
Is there an anti GFP antibody for Aequorea victoria?
Anti-GFP Antibodies Green fluorescent protein (GFP) is a 27 kDa monomeric protein, isolated from the jellyfish Aequorea Victoria that auto-catalytically forms a fluorescent pigment. GFP is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression.
What's the difference between a GFP and a YFP?
The main difference between GFP and YFP is that GFP exhibits green color upon exposure to light ranges from blue to ultraviolet whereas YFP exhibits yellow color upon exposure to the same light. Furthermore, GFP is originally derived from the jellyfish, Aequorea Victoria while YFP is…
What's the difference between GFP and YFP in a jellyfish?
GFP refers to a protein that glows green under fluorescent light and found naturally in the jellyfish, Aequorea Victoria, while YFP refers to a genetic mutant of green fluorescent protein (GFP).


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Welcome new interns and residents across the globe! Now is a perfect time to join our contributor community and help make acute medical knowledge open and readily available to all.
When the shoulder is anteriorly dislocated, two additional nerves, the axillary (supplying sensation to the lateral aspect of the shoulder) and the musculocutaneous (supplying sensation to the extensor aspect of the forearm), also should be checked.

subset: commoncrawl


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tv News RT December 9, 2021 2:00am-2:31am EST
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ah ah, no longer of beacon of democracy and perhaps never was a major international poll reveals startling thoughts on america's image abroad, especially if most countries surveyed are about to take part in a democracy summit posted by the us. the us treasury threatens american journalists with heavy fines if they work for certain publications. one author daniela's a shares, his story with us us treasury his powers. this is a north of that of an individual freelance journalist. it was microscopic that some of them are so frightened, they are unwilling even to give interviews like this one. and the number of countries registering the new oma kron strain of coverage more than doubles to 57 since last week. the w h. i talked to
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r t about the threats it poses i'm really hopeful that 1st of all, the acts vaccines will work reasonably well. and secondly, that this doesn't prove to be a much nasty virus than the earliest trained. ah, hello there with global news and international analysis, this is our t live from moscow bomb calling break. with this i was updated to happy with this. first, then over a 100 nations are going to take part in a democracy summit over the next 2 days, hosted by us president joe biden. and america is setting the bar, dividing the world into who it thinks of the good and the bad. something that china says it's got no moral right to do napalioni the theodore gotcha. the u. s. claims to be a champion of democracy and human rights, but has hidden its dark past and hypocrisy to day. the u. s. has no moral authority
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to judge whether the rest of the world is democratic or not, no matter how the u. s. glosses over itself, it's true, face of seeking her gemini, under the guise democracy has long been exposed to the world. the so called summit for democracy will only go down in history as a manipulator and saboteur of democracy with a head of the gathering over 16000 people from around 16 advanced economies to power in major research on america's image abroad. and the results don't make good reading for washington. more than half of respondents don't think the united states is a good example to follow with almost a quarter saying it never has been of a more about what else is apparently incompatible with democratic values. his art is kennimore, ben. the u. s. government sponsored summit for democracy has already begun. joe biden is presenting it as his glorious call to spread freedom and human rights around the world. in the lead up to it, a national anti corruption program was announced. our real estate markets are at
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risk of becoming a safe haven for criminals, clipped to crafts and others, seeking to pock corrupt profits by the planners say that money laundering the the sale of paintings is very hard to regulate. this is something the white house may be very familiar with. joe biden, son, hunter biden, has just discovered some artistic talent. his paintings have sold for half a $1000000.00, not bad for someone who just started painting, although not everyone is impressed. the 3rd painting you may recognize this name is a 100 biden. now you may think that such an exclusive, i'm that, that 100 binders and such exclusive company that he would have a background, you know, artistic training for example. but you would be wrong if you thought that. and you might think that he had some sort of apprenticeship with a world renowned artist, but you would be wrong again if you thought that, or perhaps that he has been selling his works for years. and again, unfortunately you would be wrong, this is not just $100.00 buying story. it is
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a bite and family story and it's corrupt. and they're basically thumbing their noses at at, at everybody because nobody seems to want to hold them into account. hunter biden is a jack of all trades. it would seem. he got paid $50000.00 a month by bruce ma holdings, a company in ukraine, even though he had never worked before in the gas and oil sector. and some people looked into this and thought maybe his father got him the gig, and that does not exactly re give squeaky clean politics. however, papa biden has always denied any foul play. we have great confidence in our son. i am not concerned about any accusations been made against him. it's used to get to me. under biden, also owns 10 percent of the chinese investment company, known as b h r. though he now announces he intends to sell his stake. when asked to clarify the relationship gen saki, the white house spokesperson didn't have much to say. the president, son is not an employee at the federal government. so at pointing to his
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representatives, joe biden just recently gave quite a bit of good publicity to protera electric bus company. now it just so happens that jennifer granholm, who he appointed as secretary of energy, is a major shareholder. given that status the up to $5000000.00 in value of investment, the secretary grunt home continues to hold and prosper. ink is position to increase in lights for personal on substantial involvement in an aggressive, wide ranging on tireless public relations campaign. to promote electric vehicles, batteries on charging infrastructure with jo biden's, democracy extravaganza kicking off. it's worth noting that recent polling data shows very few things. you asked democracy sets a good example. we decided to ask americans how they view their national leaders. do you think the american political lead is corrupt? sometimes not always. it depends on who the candidate is in which party you support
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. yeah. well, they're all serving their own self interest. and then on the american interest, the american people's interest at heart. do you believe the corruption allegations against hunter biden? yes. if you read the news and you listened to see in an you'll see how biden just did everything to cover up for a son. and they were more open about it and actually investigating it and found that there was nothing, then i'd okay, i'd be fine with that. but the fact that they're hiding everything makes me more suspicious. lots of big talk from the biden administration about fighting corruption around the world. but perhaps they should take a look a little closer to home caleb bobbin r t new york. george sallie from a london based thing tank on an international the says says the sudden buzz around corruption is just another tool to control other countries. this sudden preoccupation with corruption is a way for. busy the united states to control other countries in particular control . busy that financial transactions,
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and this is the piece with this democracy summit, the united states. a government is now under the control of joseph biden. and he's the department of justice. is treasury department all take the the from the president, and then i'm going to investigate from the by many will then they investigate the bike. all of this time is financial transaction and his son extreme success as an office. i remember by the announced what was the sometime in the fall . busy of 2020. i think that well, he's been told that he's under investigation by the federal government. well, time is the last and this investigation is obviously going nowhere. american journalists are being threatened with massive fines if they work for what a perceived as the wrong publications to contribute to a site called the strategic culture foundation have received stern warnings from
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the us treasury. this letter was sent to daniel as our own, to michael echo both new york based journalist. it says the s c f is on a list of band organizations accused of election meddling, and that us citizens who engage in transactions with the foundation liable to find that at least $300000.00. but then you know that it's check his personal experience with us. it was a warning regarding future articles. so essentially, if i write for sci fi and gauge and any kind of transaction, which i assume means any, an exchange of money i could be had with massive fines that were completely destroy my finances. so i was taken aback. i was, you know, concerned certainly, but actually i stopped writing for s c f since the previous, you know, 4 or 5 months earlier. so i didn't feel personally threatened. but others who, who want to continue writing for the f works dreamily frightened fair. they have all stopped writing from the site because really there's just, it's, you know,
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it's impossible to, to battle the us treasury in this kind of case. the us treasury is power is, is, is enormous. and that of a, of an individual freelance journalist is, you know, microscopic that some of them are so frightened, they're unwilling even to give interviews like this one. so you know, so this is what it's showing effect means they're, they're unwilling to write and they're unwilling even to talk about why they, why they're too afraid to write the threats makeup. part of a targeted sanctions campaign announced by the treasury secretary in april. treasury will target russian leaders, officials intelligence services and their proxies that attempt to interfere in the us electro process or subvert. he has democracy. this is the start of a new us campaign against russian line behavior. the u. s. government claims the strategic culture foundation is controlled by russians, a foreign intelligence agency, the s t v r, and that it's also linked to the russian foreign ministry. the foundation denies
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this and calls itself a research platform on your ration global affairs where we've contacted the see to get a further response to the claims. we also waiting to hear back from the us treasury to, well let you know if we get an answer daniela's that says that a dangerous precedent has already been set. i think that the, that the at the s e s. the foundation hosts honest journalism. even though out i don't, i don't agree with much of it. this is, this is serious work by serious journalists. and that as a supporter of a free speech in a free press, i believe that readers should have access to this. ready kind of stuff and, and yet the, the u. s. government is trying to close it down. and therefore trying to stop information, which is usually at variance with official us policy. from reaching from reaching audiences, including audiences in the us, to stop journalists from writing for these kinds of websites as a new step. and i think that,
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and i was surprised because because the united states, in fact has a very good track record on when it comes to a free press. i mean, in america we can say anything we want, you know, i can write that with the most outrageous things about george w bush or joe biden, and not have to worry for a moment about the search secret police crashing through my door. but now. 2 things may be changing slightly, and it doesn't the order. well. the number of countries where the due covey, the variance omicron is registered, has more than doubled in just over a week. the world health organization says the strain has been found in 57 countries so far and new policies are being introduced. the thing is that the rate of hospitalizations could now rise. we also w h o special ed boy on cobra for his perspective on the very end. people do not understand enough about this paris. nobody knows for sure how nasty
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omicron is, but there's information coming through that it's, it's not too awful and this is good. but it takes time to sort these things out and we're in a kind of odds on stage at the moment where we're breaking for the results of the research. what i want to say is everybody keep doing the stuff that you know what to do, even if you're bored a bit. and sooner or later we'll find out how bad only kron is. some companies saying they're vaccines will work other saying they can a need a booster for them to be effective. all this will come clear, it usually takes about 4 weeks and i anticipate that will happen, but privately inside my. so just because you asked me, i'm really hopeful that 1st of all, the acts vaccines will work reasonably well. and secondly, that this doesn't prove to be a much nasty virus than the earliest trains. i've been involved in controlling disease outbreaks and pandemic since 2005. that's quite
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a few years. the one thing i've learned try never to force people to do things through various incentives like fines or even nastier things. mandates are a last resort and the use of vaccines in children is because that is thought that vaccination will help to reduce circulation of the barrison children. but right now, the world health organization sticks with its recommendation that vaccines should be used for people who are at risk of severe illness and death. there are still an awful lot of susceptible people around even when you've got 70 st vaccination coverage than this. hi, right of, of cases is being driven by the susceptible. secondly, there does seem to be
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a capacity for the borrowers to re infect people, especially if it is some months since they either had their previous infection or had their immunization. the protection doesn't last for life, it wears off, so that's i think, the reason why we're staying the spikes, even though it's high vaccination. mounting outrage in the u. k. over a supposed downing street party last christmas during tightened. pandemic restrictions has claimed its 1st scalp. the prime minister, former press secretaries resigned after a video, emerged showing her joking about the event suggesting that it did happen a burst. johnson has ordered an inquiry and was grilled in parliament on wednesday . they knew there was a party. they knew it was against the rules. they knew they couldn't admit it and they thought it was funny. prime minister has been caught right handed. why definitely an investigation right now. but just admitting it. i been repeatedly
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assured, oh, i probably can big news. bad news. but there is a risk of doing a great, a grave injustice to people who, frankly, obeying the rules with the prime minister. the government spent the week telling the british public there was no party. all guidance was followed completely. millions of people now think the prime minister was taking them for fools. well, many say this could be the final nail in the coffin for forest johnson as he's now being accused of lying or even trying to cover up an alleged christmas party that happened last year. ever meant the kobe to restrictions. now if you had that from the kiss tom, at the leader of the opposition, he says the tory party have been caught red handed as multiple sources. confirm that this party did indeed happen, boris johnson. he insist that he's been assured that it didn't take place,
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but if you look into the wedding of forest johnson, he didn't flat out, deny it either. now all of this comes off to number 10. officials were court joking about this, so called potty or as they phrased it, this wine and cheese business meeting has the i t v exclusive video and she recognizes i'm at home is fictional policy was a business meeting. i mean, it was not so she just now if a party did happen, well, certainly the kind of it rules were broken because at that time london was in tier 3 of the private restrictions, meaning no indoor mixing. of other households was allowed at the time. not to mention the day off to this party, christmas was cancelled, and types of restrictions were then announced. now things have really escalated throughout the day to day. in fact, the police are even considering launching an investigation into this matter,
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boris johnson says he will assist with any probe that might potentially happen. but of course, this isn't the 1st time the tory party have been embroiled in such rule breaking soccer. if we look back to the heights of the pandemic dominant cummings, the prime minister's former chief 8. well, he travelled 270 miles from london to durham to visit bernard castle. then of course, mad hancock, the former, how secretary he was seen, intimately embracing a colleague, both of which have been rule breaking. and that's really the problem. that's why, as so many people are angry that those people that are making the rules think it's ok to simply break them. while the political opposition, the house of commons, they say they simply can't go on. and the prime minister's time is up downing street willfully broke the rules at the moment. the sacrifices we have all made, the prime minister has our duty, the all my right and moral choice left to him and his for his resignation.
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countless children now growing up without parents while parties were held at number 10. it is an insult for everyone. he wasn't allowed to say that final. good bye. my question is very simple. ah, how does the prime minister sleep at night? well, the reality, as many people were in absolutely heart wrenching position last year, people that couldn't go and visit their dying parents simply because they wanted to stick to the rules. in the meantime, the tories were allegedly out partying of former british home office minister norman baker says the scandal will spur further distrust in the government. it was, it was a party by definition, the rules were broken. so i think he's lost all credibility. i think frankly has become a liability not just to this country but to the conservative party as well. but i
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think his own party after defending him, being given aligned to parents to the media o defined they've been live the gotten passed by the prime minister. so the primary doesn't happen over over bogging uncomfortable troops. he wants to be loved. he wants to be like to provo, promises, and delivers. and there is a smell of arrogance about this government. they can do what they want to abandon the laws of the people. i think we ought to have a plan b company over this winter, and that's like increasingly likely, even today, then people are around the country going to say, why should i bother her full in the roles with the private doesn't? so the consequences for public health are potentially serious as well. without he for moscow still ahead for you this, our president biden's migration policies in the spotlight after a slumping deportations and the re introduction of a trump air of policy that led to a surge of the border in arizona. it's our next story after the break.
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ah ah, is your media a reflection of reality? ah, in the world transformed what will make you feel safer? high selection for community. are you going the right way or are you being led somewhere? direct? what is true? what is faith? in the world corrupted, you need to descend ah, to join us in the depths will remain in the shallows.
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join me every thursday on the alex salmon show. when i was speaking the yes of the world politics, sport, business and show business. i'll see you then. i see the governor of the u. s. state of arizona's calling out the biden administration over its migration policies. he says, border patrols are overwhelmed. national guards have been called up because of what he calls biden's piecemeal policies. the biden administration's miss direction is encouraging migrant families to take a long and dangerous journey as thousands arrive at the border. local law enforcement is overwhelmed with processing. this search creates a safety and humanitarian crisis for both arizonans and migrants. deportations have
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dropped by around 80 percent during the biden presidency. according to a migration control thinktank. the center for immigration studies has been analyzing data from the federal agency that handle the legal entry known as ice. a notice that the deportation of criminals also fell. a lead director of policy studies at the group behind the report explain to us what's happening with us migration were at a time of historically high, illegal immigration, and historically low enforcement when ice agents are not allowed to make arrests, as they were in the past, under the new policies that means that the serious criminals that are typically the priority for enforcement are allowed to stay in american communities instead of being sent back to their homes. and when criminals are allowed to stay here, typically they re offend and create new victims and continue to cause problems in
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this country. so this is an unnecessary public safety risk and is odd damaging communities that have to absorb criminals who otherwise would be sent home. that said, the biden administration has bent on the pressure and reinstated a trumpet program where migrants to return to mexico to await their asylum decisions are the announcements of the re loans sent masses of desperate people to the us border to try and get in before it started working again and the scramble we just heard about in arizona or jessica vaughn says, more to be done to prevent an eagle crossings. this is a matter of policy. it's not a matter of resources or immigration system is badly in need of reform. we need to have measures in place that result in more enforcement, more tools for immigration enforcement, more on programs that allow state and local law enforcement agencies to help ice do
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its job. and i think we need more money for enforcement directly at the border as well. more barriers to prevent people to keep, to cross illegally on a desperate attempt to save itself from a p r disaster over struggling economic policies. the biden administration has reported, they tried to reshape its media coverage. a senior c n n correspondent dropped a bombshell report claiming that senior white house officials a been briefing major american newsrooms on how they should cover the economy. the white house, not happy with the news media's coverage of the supply chain and economy has been working behind the scenes trying to reshape coverage in its favor. senior white house and admin officials have been briefing major newsrooms over the past week. as ballooning prices and supply chain problems had american consumers, the biden seems been slammed for its economic policies. and apparently, instead of fixing the economy,
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the white house is resulted to mitigating the damage by trying to influence reporting. cnn itself rolled at a few optimistic headlines, openly praising the president's agenda. so i'm going as far as stating that inflation can actually be good for every day americans onto the public though it's finding that optimism a little suspicious. this sounds a lot like the start of a state propaganda rollout and the media is just browed lead meeting. they are part of it. cnn is actually bragging about this, which means c, n, n, n y, c m s, and b, c, y po will, all in attendance, ready to take orders. american journalist and commentator chadwick mole claims and see and then just pretend to give bite in a tough time. what does make it strange that it is so proudly reporting on this? it brings them trying to reaffirm their commitment to neutrality and to pretend like they've been getting the destruction that time when it's nothing compared to what they did. the last president,
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cnn was one of the very powerful forces out of many that worked tirelessly to get this president elected and to trash and slander the former president into oblivion or so they caught me. don't forget that. during the 2016 election, we had all those e mail leaks that showed that the hillary clinton campaign was colluding with reporters and presenters on cnn data about what questions asked during debates and how to frame certain things. and that could be what we're sitting here with the bite administration. they show up to see and as offices, and they explain, you know, they're unhappy with the bad reporting. the for boarding president isn't that bad? i think the real situation is probably much worse. the real economic situation in this country than even seeing that is reporting you should invest more in its military development to avoid the risk of lagging behind other major powers. thus, the voting from the blocks from policy chief who says a significant gap already exists. we could have been union and
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a member of state. we need to do much more to that. and we need to do much more together. right now. we cannot wait because the choice for as is simple. either we invest a lot on defense innovation, or we will become defense irrelevant. according to the european defense agency, the body responsible for military cooperation and development within the block. the a you spent a record 198000000000 euros on defense last year. even despite pandemic, hardship. however, only one percent of that was allocated to the research and development of new technologies when the goal was to spend double that amount. what's more, it 7 times less than what america spent. now, among other proposals to strengthen the ego military, there's also a document known as strategic compass, which revives the idea of
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a creation of an independent european army that could be rapidly deployed to tackle any crisis on the paper. also on the lines, the necessity for a common approach to military issues and increase spending on it. now a german and pay the we spoke to says the supposed military threat is far from being the use main challenge right now from the beginning of the developing of the european treaty, there was always included the idea to have a own strong military arm that they are most of the european union now of the military thinking very much developing and the european union though they are pushing for have, having higher expenditure on military issues and to have more who are in military you spell that for a policy. the question is protected against tool. i don't think that the european union is at the moment under current stretch from,
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from military terms from anybody we. ready have problems with it, but i don't see that anybody is now trying to attack the military european union. those are the main stories were across here at r t. you can get more on our website is what is more video and award winning shows on our youtube channel for now though, from calling bro moscow. thanks for watching. ah ah. back geysers financial survival, guy, housing, bravo. oh, you mean as a downside, artificial mortgage?
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ruth, dr. carried away was calling to report ah well come to the alec salmon. sure. where we examine the consequences of cop 26 in glasgow. was that much too late and much, harold did summit, a success or failure and as our planet saved or to undo these times of crisis, as stimulates great art, we talked to 2 young are to be a cut and stared class whose work has been inspired by the climate emerging on the 8th of the glasgow summit, we asked you at hazel dean, professor carbon capture on storage at the university of edinburgh for his hopes
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Due to personal circumstances the situation has changed and we are closing the project down at the end of this month until April next year.
We are bringing a famous ocean racing catamaran back to life. I rescued this famous boat from being chopped up by the previous owners and am committed to preserving this piece of sailing history. We are using recycled materials wherever possible and our aim is to create a comfortable fast cruising catamaran which we will then sail to exotic locations around the world.
Our schedule is laid back but we do like a sense of achievement at the end of the day. The most important part of a big project like this is that we have fun whilst doing it. So we are always playing good music, eating well and experimenting ideas and so far it's been pretty effective. There are a lot of individual jobs to be done, however, it's a team effort and there should be no stress whatsoever on the worksite!
The sun is out nearly everyday and winter is mild here.
We are planning on sailing this beauty around the Mediterranean, Caribbean, Asia and South Pacific. So if you'd ever like to come on the boat when it's rocking and rolling you will be most welcome.
Projets artistiques
Bricolage et projets de construction
S'occuper des animaux
Entretien général
The travellers will learn lots of new skills in boat repair and maintenance utilizing recycleable materials. There will also be opportunities to learn how to work with Fibreglass, welding stainless steel and sail repair.
Nazare has a rich fishing history and you will be able to get a taste of the local fishing community with the boatyard being located inside the fishing port.
The worlds largest waves are soon in season and there are also surf, jet-ski, beach football and other competitions being held throughout the year.
We are looking for people to help with a large recycling project to renovate a large ocean going catamaran to get it sailing again. The jobs range from scrubbing the decks, painting and general cleaning all the way to redesigning and creating our own outboard engines, mast and also constructing a new deck.
There is work for all skill levels and any prior boat knowledge is always much appreciated as we are figuring this thing out as we go!
We are also looking for artists to bring the boat to life!
The shifts can vary depending on the workload but overall there is a maximum of 5 hours work a day.
You can start in the morning at 9am and work until 12:30 then break for lunch and do a bit more in the afternoon or start later in the morning and finish no later than 5:30.
The work can be exciting or quite slow and laborious. Everyday is different and the jobs are always time consuming as we are figuring everything out as we go. However, if we are enjoying ourselves then this doesn't matter so much. Initiative is very much appreciated!
There is a local man working on the boat everyday from 9 and he can help with orders and expertise, those with extra skill would be more likely to run mini teams of 2 or 3. Just so everyone can learn to do things the right way.
Langues parlées
Anglais: Courant
Espagnol: Intermédiaire
Portugais: Intermédiaire
Cet hôte propose un échange linguistique
Cet hôte a indiqué qu'il souhaitait faire partager sa propre langue ou en apprendre une nouvelle.
Contactez-le pour en savoir plus.
A shared bedroom in a Nice Apartment in Nazare.
We have 2 single beds in a shared bedroom in our apartment.
There is also a double bed available this coming week.
We can offer only 1 bed for the couples. But if you love birds have got a van then no problems!
Great ocean view - we can watch the "port do sul" every night and we get a view of the big waves (when they roll in) from our doorstep.
10-15 minutes walk to main beach, swimming pool, gym and supermarkets. Close enough to hear the ocean at night :)
Altogether: 2 singles beds at the apartment
1 double bed " " "
Wifi is available in the apartment, however you might want to have your own data as 60gb can run out sometimes.
All utilities and transport to and from the worksite.
Plenty of space to park your vehicle if you have one.
Breakfast and lunch is prepared by yourself (you can help yourself to the pantry)
Dinner we cook altogether.
If you have a musical instrument or surfing gear, bring it along! There are numerous surf schools in the area that will take you surfing to the best surf spots each day.
Accès Internet
Accès Internet limité
Nous avons des animaux
Nous sommes fumeurs
Familles bienvenues
We have mobile internet with 60GB data per month available from a mobile hotspot.
Any size of van can be accomodated
Plus de 2
5 hours a day, 5 days a week
Workaway s'est engagé à verser plus de 20 000 USD, à travers sa fondation, pour aider les réfugiés ukrainiens.
Sur demande, tous les workawayers basés en Ukraine pourront obtenir gratuitement la prolongation de leur adhésion, jusqu'à nouvel ordre.>
Les hôtes en Ukraine ont été temporairement désactivés pour des raisons de sécurité.>
Si vous êtes hôte et si vous pouvez accueillir des réfugiés, précisez-le dans votre profil et nous vous ajouterons à notre liste d'hôtes last minute.
Si vous voulez faire un don et aider, vous pouvez cliquer ici.


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If you are in the market for the toilet bowl cleaner for hard stains, you may be wondering where to start your research. Moreover, how do you know which one is the best fit for you?
To help you find the best toilet bowl cleaner for hard stains we analyzed & researched all the features that are right for you and came up with a list of 10 different options to purchase.
Analyzing the total market, we have listed the following best toilet bowl cleaner for hard stains regarding the user experience, materials, durability, strength, top-notch performance, budget, preferences, and so on facts based on any decent user's taste.
- [5X CLEANING IMPROVEMENT] - Efficiency, easy and effective toilet bowl cleaning. This new pumice cleaning stone brings a 5X cleaning improvement to make it easier for you to remove the stubborn stains, hard water rings and other dirt, an efficiency cleaning tool for toilet bowl, grilling rack, pool tile, meeting your various needs.
- [HIGH QUALITY MATERIAL] - This toiler bowl cleaning tool is built with natural pumice stone which features non-toxic, odorless and BPA free, no more pungent chemical cleanser. The pumice cleaning stone is totally safe and friendly for human beings, pets, plants and water, and it never leave scratches on the surface of the ceramic tile and metal.
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- [WHAT YOU GET] - Package includes 2 pack toilet bowl pumice cleaning stone in dimension of 9.25"x1.38". We're devoted to provide high quality and practical household accessories to ease your housework, 30 days money back, 1 year warranty, feel free to contact us if there is any issue with our pumice cleaning stone.
- Best on Rust: #1 Heavy-Duty Rust Stain Remover Brand
- Specifically formulated to repel rust and hard water with every flush of your toilet
- Convenient rust stain solution; Long-lasting formula prevents rust stains from forming in toilet bowl for up to 45 days
- Safe for use with all toilets, internal toilet components, plumbing and septic systems
- Simply drop tablet in toilet tank and let Iron OUT do the work for you
- Use Iron OUT powder or liquid spray rust stain remover to clean the bowl before inserting tablet in tank
- ZEP Acidic Toilet Bowl Cleaner, 32 Ounce (2-Pack)
- ZEP Commercial Acidic Toilet Bowl Cleaner quickly removes rust stains, organic and hard water stains and residue. Its clinging gel formula works longer and harder on vertical surfaces, leaving a clean shine and fresh fragrance. It is safe for septic tanks, drain lines and water treatment systems.
- Clinging gel based acid formula. Specially formulated to require less scrubbing
- Built-in squirt top. Removes soil build-up, lime-scale and other stains
- Minty pine scent. Deodorizing formula
- INCLUDES STORAGE CASE – NEW!! Now Powerstone Powerhouse Pumice comes in a sturdy storage case! When finished with cleaning toilet bowls, sinks, tubs, tile or other surfaces, simply rinse and store in the convenient case. Includes air vents to allow drying.
- REMOVES STUBBORN STAINS QUICKLY AND EASILY – Power through calcium deposits, limescale, hard water rings, iron deposits, rust stains, and more on toilet bowls, tubs, sinks, tile and other porcelain and ceramic surfaces. Plus, it's more than 20% more dense than similar products to last longer and clean better.
- ECO-FRIENDLY CLEANER – Tough enough to remove stubborn stains yet is chemical-free, odorless, non-toxic and eco-friendly ... it is made from recycled materials.
- PROTECTS YOUR HANDS – Includes a heavy-duty plastic handle to easily grip while cleaning…no gloves needed! Just use gentle back and forth motions to remove stains and deposits. The fine-grit, white pumice stone leaves behind no residue on surfaces or on your hands.
- CLEANS MULTIPLE SURFACES – Use on toilet bowls, tile, sinks, tubs, and other porcelain or ceramic surfaces. Also cleans baked-on food from BBQ grills, dirt and grime from gardening tools, rust from workshop tools, as well as other surfaces. NOTE: test on small area to determine scratch resistance.
- 𝗣𝗢𝗪𝗘𝗥𝗙𝗨𝗟𝗟 𝗦𝗧𝗔𝗜𝗡 𝗥𝗘𝗠𝗢𝗩𝗘𝗥: Our patent pending liquid pumice stone toilet bowl formula breaks down and removes hard water stains, rust, lime scale, oxidation, and mineral deposits from your porcelain sinks, tubs, and bowls.
- 𝗘𝗔𝗦𝗬 𝗧𝗢 𝗨𝗦𝗘: Just apply Megabrite Toilet Bowl formula and scrub gently, rinse, and watch the pumice stone gel leave porcelain surfaces sparkling clean again!
- 𝗦𝗔𝗙𝗘: While other toilet products contain damaging harsh chemical, Megabrite toilet cleaner contains no chlorine bleach or harmful chemicals. Our cleaner is biodegradable and formulated with 100% essential oils.
- 𝗣𝗨𝗠𝗜𝗖𝗘 𝗦𝗧𝗢𝗡𝗘 𝗚𝗘𝗟: Pumice stone gel is simple to use - just apply, scrub away the mineral deposits with included scouring sponge, rinse, and enjoy your clean mint scented bathroom.
- 𝗣𝗟𝗨MB𝗜𝗡𝗚 & 𝗦𝗘𝗣𝗧𝗜𝗖 𝗦𝗔𝗙𝗘: Our product is made in the USA and performance tested and safe for septic tank systems.
- Iron Out Automatic Toilet Bowl Cleaner is designed to repel rust and hard water stains with every flush of your toilet bowl.
- The concentrated formula prevents and repels new stains from forming for at least 45-days per tablet.
- Laboratory tested safe for septic systems and all toilet parts
- Repels rust with every flush
- 💚【Easy to remove stubborn stains】Natural pumice stone scrubber has a unique abrasive power that easily cleans hard water rings, limescale, rust and iron to give your toilet a new look without any scratches.
- 💚【Extra-long handled cleaning brush】8.2 Inch longer handle keeps your hands free of stains and avoid injuries, and get your place cleaner with less effort.
- 💚【Multifunctional toilet brush】Unlike other cleaners, pumice is non-toxic, odorless, chemical-free, and can change its shape for use on grills/tile/bathtubs/kitchen surfaces, etc.
- 💚【Premium material】Upgraded pumice and handle will be stronger and clean better. A new green package includes 2 pumice stones for cleaning the toilet and 2 hooks for hanging. What a great deal, 4 items for one price!
- 💚【Easy to use】There will be a little dust after opening the package, but it will not affect the use, so please wet the pumice stone before use, then wipe off the stains, please clean it, hang it on the hook to dry for next use.
- 💚【A 365-days-quality guarantee】Due to the special material of pumice, it may be damaged in transit, if you encounter product dissatisfaction, please feel free to contact us, we will be the first time to serve you!
- 🚽 EASY STAIN REMOVAL: Harness the abrasive power of pumice stone cleaner to remove hard-water stains, limescale, calcium deposits and porcelain fixtures. Our pumice toilet brush not only great for toilet bowls but also works on tiles, bbq grill, pools, bath tubs and ceramic surfaces. Make your toilet bowel brand new without any scratches on the surface.
- 🚽 CLEANER PUMICE: The toilet pumice stone is made of pumice which is tough enough to remove stubborn stains.
- 🚽 POWERFUL PUMICE POWER: Our pumice cleaning stone with cleaning power you won't believe. This pumice stone cleaner will tackle stains and water deposits that that traditional brushes and other cleaners cannot wash.
- 🚽 QUICKY & EASY : Each pumice stone can be sharpened into different shapes to clean the narrow edges and corners that other cleaning tools aren't able to reach. Just dampen the pumice before use, then apply gentle pressure in straight motions. It's that simple !
- 🚽 PUMICE STONE WITH HANDLE : Pumice stone for toilet provides ergonomic grip to maneuver during cleaning. Keep your hands clean while using the pumice stick. Toilet pumice with plastic handle and no gloves needed!
- Natural, Chemical-free Cleaning - Remove stubborn stains like magic with our Pumice Stone Toilet Scrubber. This toilet cleaner brush is made from real volcanic pumice stone for cleaning tubs, sinks, and toilets without using harsh chemicals.
- Removes Stain with Ease - Trust our toilet bowl cleaner for hassle-free stain removal. The scratch-free toilet pumice stone with handle and cleaner eliminates blemishes while protecting your toilet from damage.
- For All Types of Stains - This shower cleaner brush gets rid of markings from hard water, calcium, lime, and rust. Get that better-than-new bathroom look with our tub scrubber with long handle.
- Not Just for Toilets - A multipurpose water spot remover, this toilet bowl wand can also be used to take away rust from hard-to-reach corners and porcelain sinks and tubs.
- Functional Bathroom Scrubber with Long Handle - With a 12in extended handle, our marble stain remover keeps your hands clean as you scrub.
- 🚽【EXTRA-LONG HANDLE】Upgrade design for toilet pumice stone handle, longer handle saves effort, easy to clean, keeps hands away from stains, extremely clean.
- 🚽【MULTIFUNCTIONAL CLEAN BRUSH】You can change the pumice into other shapes by yourself, it can be better adapted to different scenarios, such as a bathroom, swimming pool, kitchen, household cleaning, etc.
- 🚽【NO STAINS, PRETTY CLEAN】Effective cleaning with a pumice toilet brush, its unique abrasive ability can remove stains quickly and easily, can clean hard water ring/scale/rust/iron.
- 🚽【NATURAL PUMICE】Pumice is a porous volcanic rock, non-toxic, odorless, acid and alkali resistant, corrosion-resistant, will not scratch your toilet, tile, bathtub, and other surfaces. An eco-friendly cleaner for toilet cleaning.
- 🚽 【2 PACK 2 HOOK】One package has 2 pumice stones for toilet cleaning and 2 hooks for hanging. TIPS: Due to the special material of pumice stone toilet brush, there will be slight chipping after opening the package, but it will not affect the use.
- NOTE: The pumice stone may be broken or loosened during transportation, if this happens, please contact us without hesitation, we'd happy to serve you, hope you have a wonderful shopping experience.
Know Your Need
Before making a decision for buying any product, you need to know about what your actual need is. If you don't know your need, you can't purchase the right product.
Firstly, you thoroughly check whether you really have any need of buying any torch light for dabs. If so, take a copy and write down your all necessities.
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Budget Friendly
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The durability of a product is a measure of how long it will last under normal use. Durability is often a function of the materials used in the product, as well as the design and manufacturing processes.
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Finally, check the warranty to see how long the manufacturer expects the product to last. So, checking durability is a must issue for purchasing the best toilet bowl cleaner for hard stains.
Assess the Features
When you are considering purchasing the toilet bowl cleaner for hard stains, it is important to assess the features of that product to ensure that it is the right fit for you. By taking the time to do this, you can avoid wasting your money on a product that does not meet your needs or expectations.
At the end of the day, we all purchase a product of any category which would be a good investment, right? We hope that our in-depth review for the best toilet bowl cleaner for hard stains will assist you to quickly finding the right item for your necessities.
Last update on 2022-08-10 / Affiliate links / Images from Amazon Product Advertising API


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By Iain Withers and Sinchita Mitra
LONDON (Reuters) - The Bank of England is satisfied lenders have taken steps to ensure they are no longer "too big to fail" in any future crisis, it said on Friday, though it did find shortcomings at three leading banks.
The BoE is aiming to stop banks from requiring taxpayers to bail them out, as happened in the 2007-09 global financial crisis.
The central bank said it was satisfied overall that banks could be wound down safely while keeping vital services open, with shareholders and investors in line to bear the costs rather than taxpayers.
In its first public assessment of how failing lenders could be dismantled in a crisis, the BoE said it had also identified "areas of further enhancement" for six companies.
The three banks found to have shortcomings were Lloyds (LON:LLOY), Standard Chartered (OTC:SCBFF) and HSBC.
All three were found not to have produced sufficient analysis of their liquidity needs were they to be wound down.
Globe-spanning banks HSBC and Standard Chartered were also found to have failed to produced up-to-scratch restructuring plans.
The central bank said the shortcomings identified would complicate its ability to undertake a resolution but it could still do so safely.
In separate statements on Friday the three banks said they were making enhancements to address the issues identified and were improving their so-called resolution plans.
"Safely resolving a large bank will always be a complex challenge so it's important that both we and the major banks continue to prioritise work on this issue," said Dave Ramsden, the Bank of England's deputy governor for markets and banking.
The other lenders included in the review were Barclays (LON:BARC), NatWest, Nationwide, Santander (BME:SAN) UK and Virgin Money (LON:VM) UK.
Analysts cautioned that it is unclear how well the plans would work if they ever had to be put into action.
"Some will be sceptical as to whether the resolution framework would work exactly as intended in practice in the event of a failure of a high street lender, given the enormous losses it could result in for shareholders and debt investors," said Goodbody banking analyst John Cronin.
The BoE said it would repeat its assessment in 2024 and review progress made by the lenders every two years after that.
The central bank has powers to force lenders to make structural changes if it feels there are barriers to fast and orderly closure.
Publication of the review was delayed by a year to free up lenders to deal with the COVID-19 pandemic.
In 2018 the U.S. Federal Reserve said that the U.S. arm of Barclays had shortcomings in its resolution plan, but not deficiencies that required a bigger capital buffer.
By William Schomberg LONDON (Reuters) -Britain borrowed more than expected in July, official data showed, underscoring the challenges facing the country's next prime minister to...
By Martin Quin Pollard and Eduardo Baptista BEIJING (Reuters) -China's cyberspace watchdog wants to build an "affectionate" relationship between internet enterprises and the...
By David Milliken LONDON (Reuters) -British shoppers spent more than expected last month as many were enticed by online shopping promotions, despite a weaker picture recently as...
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- Активен с: 20.06.2022
- https://writeablog.net/planegun9/deep-tissue-massage-is-it-right-for-you
The benefits of deep tissue Massage
Before going for the massage, make sure you have a large amount of water and a light snack. Following a massage that is relaxing, your body will rush to absorb and eliminate the excess water. In order to flush out the toxins leftover following a massage It is essential to consume plenty of fluids. For hydration then you should take a snack. Massages can increase the flow of your circulation and digestive systems. 출장마사지 Therefore, you should try eating a balanced meal after having a relaxing massage.
Massages for the back can benefit those with a medical condition. Deep tissue massage may cause constant soreness which makes it difficult to manage. For this reason, it is recommended to take a complete medical history before beginning a massage. Likewise, your therapist should be aware of this before commencing. This is to the advantage of both the person receiving the massage as well as the massage therapist. When you are considering an massage, ensure to speak with your physician if you have any heart problems.
Massages aren't just beneficial for maintaining a healthy circulation system, but they can also assist in preventing and easing persistent pain. By stimulating circulation, massages bring more oxygen and nutrients to your organs. Massage therapists can help activate your nervous system and improve your lymphatic system during the massage. Your body will be able to combat harmful bacteria and viruses. Whether you're looking for the benefits of a massage deep into your body for relief from stress or relaxation the therapeutic massage can enhance your life and make you feel happier.
Massage for deep tissue can be misinterpreted as the release of the toxins. Though this could be true for a small number of people, the truth is not universal. Deep tissue massage can be beneficial for your body, decreasing pain and soreness. But it may also cause injury for the person who is performing it. It requires a lot of handwork and isn't good for the hands. Massage that is deep tissue requires you stay clear of any conditions that may cause your hands to become painful or painful.
Massages are a great way to ease chronic pain and improve circulation throughout the body. It is possible to use your hands to get deep-tissue massages as well as lower blood pressure. You will also feel better general. Massages can be great for anxiety relief and can boost your immune system. In addition, massages helps you sleep better and get better sleep. It will help you to feel more relaxed! It helps you in everyday activities.
Deep-tissue massages must be avoided by people with any health history or medical issues. They may cause an edema called venous thromboembolism. A blood clot can form on the arm, leg or the groin. The clot can eventually spread to your lungs. Prior to receiving massage therapy, it's recommended to talk with your doctor if suffering from embolism of the venous system. If you're concerned regarding the health of your body, it's advised to seek advice from an expert.
Massages can improve your overall wellbeing. Massages can help relieve anxiety and boost circulation. Massages are a great way to transfer blood away out of congested or damaged areas and allow new blood to flow. This is an important part of massage therapy for many patients. This can be a fantastic choice for those suffering from chronic pain or who need to undergo more intense treatment. The type of therapy is often covered by health insurance coverage. This is especially beneficial to patients with chronic illness So if you're experiencing issues, talk to your doctor or healthcare provider.
Massage has many other advantages for health, in addition to increasing circulation. Utilizing pressure using your hands helps to eliminate lactic acid in muscles, which enhances the functioning of the body. Massages can also improve lymphatic circulation, which means less blood pressure and a better body. Also, it will reduce your chances of developing embolisms in the veins. This is a very serious condition which can cause strokes, even though it's not uncommon.
Massage can help ease pain and also increase psychological and physical well-being. Massage can ease the symptoms of chronic constipation, arthritis and fibromyalgia. A massage can help to feel better. But the benefits of a massage don't stop there. Massage may improve your mood. It can also help with the fatigue that is chronic. Deep tissue massages can help people feel more relaxed.


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All the plants are fully rooted, full and mature, except Cuttings.
Plant's height includes pot/Soil.
Plants in 4in or 6in pot are 3-12 months old
Plants in 1 gallon pot are 2-4 years old (1-2.5ft tall for Tree )(3-6" for ground covering(Sh4 ft tall. Tree are about 3-6 ft tall). We may trim the plants to 3-4 ft prior shipping
Most of Plants are shipped within 1-3 days. Some plants may take 10 days to get the Permit. No plastic Pot is Included . We remove 1/3 to 1/2 soil before shipping.
Warranty: We offer 3 days warranty upon arrivial
Some small damage such as minor broken branches, less than 30% dry flowers etc may not be reimbursed. .
The Tulbaghia Silver Lace Plant 1Gallon Tulbaghia Violacea Silver Lace Plant 1Gallon Society Garlic Lavender Live Plant Outdoor FM7 is evocative, to say the least, but that's why you're drawn to it in the first place.
Inventory Last Updated: Aug 19, 2022
Inventory Last Updated: Aug 19, 2022


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Car Park Space / Covered / Secure / Uncovered
Convenient CBD Parking on Stout Street
Wellington Central City Parking. $470 per month
Listing informationListing information
9 Stout Street
Wellington Central - Wellington City
15 m2
Total Area (m2)
2769 Views
Listing informationListing information
9 Stout Street
Wellington Central - Wellington City
15 m2
Total Area (m2)
2769 Views
Covered car parking available now at 9 Stout Street. Using our easy and convenient License plate recognition technology for entry and exit.
$470 + GST per month on a monthly term.
Monthly parking offers the convenience of unlimited entry and exit to your car park and one easy
monthly payment.
Primeproperty has a number of car parking options available in fantastic central city locations
including casual parking. We pride ourselves on offering secure and convenient car parking at very
competitive rates, along with exceptional customer service.
Contact Us today!
Listing Number: 8704
(Mapped location may not be exact)
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