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25+ Best Ideas about Wine Fridge on Pinterest
Project Info
Category: Wine Fridge Bar Cabinet
Date: June 19, 2019
Site URL: www.pinterest.com
Project Description
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subset: commoncrawl


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Global Market Insights, Inc. adds new Ionic Liquid Market report which provides in-depth information about Industry overview, top vendors, Key market highlights, product types, drivers, challenges, trends, industry landscape, size and forecast.
Germany has been pioneering for clean energy for sustainability coupled with growing energy storage industry, this should positively impact regional IL industry growth.
U.S. ionic liquid Market is estimated to reach USD 750 million by 2022, owing to stringent regulations set forth by prevailing agencies safeguarding interest against toxicity and growing health concerns should drive regional product demand.
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Ionic liquid (IL) is a form of salt in liquid state and is largely made of ions or short-lived ion pairs. It is also known as liquid salts, liquid electrolytes, ionic melts and fused salts and is mostly used in electric battery and sealant applications owing to its low vapor pressure.
Rising trend of adopting green solvent due to increasing toxic concerns from their organic substitutes should drive ionic liquid market during the forecasted timeframe. Stringent environmental regulations, policies and pressure set by agencies should drive global market.
Ionic liquids are extensively used in bio catalysis and bio transformations owing to its negligible vapor pressure, thermal stability and controllable viscosity with water or organic solvents. IL's are used as solvents, co-solvents and reagents propelling product demand.
Ionic liquids have been subject of interest recently due to its low volatility and ability to dissolve a wide variety of compounds, making it useful as green solvents for energy storage application and industrial process. IL's perform as electrolytes in products such as dye-sensitized solar cells, actuators, thermos-cells and double layer capacitors conducting electricity with elongated reaction times, pivoting global ionic liquid market.
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Ionic liquid is also used to solve critical pharmaceutical problems, performing as carriers of pharmaceutical active compounds and salts through the combination of anions and cations. Pharmaceutical industry is expected to account for generating over USD 1.2 trillion by 2022 along with which IL Market is expected to grow.
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Global ionic liquid market is fragmented as large number of companies cater ionic liquid to different end-use industry. Some key players include TCI, BASF, IOLITEC GmbH and Solvionic. Other major manufacturers include Wuhu Huaren, Coorstek Specialty Chemicals and Santa Cruz Biotechnology.
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a r t i c l e i n f o
a b s t r a c t
Article history:
Received 5 August 2016
Received in revised form
4 November 2016
Accepted 9 November 2016
Available online 10 November 2016
This experiment was conducted to study the effect of various levels of ACV and Propionic acid (PA) on
expression of immune related genes and growth performance in white shrimp (Litopenaeus vannamei).
Three hundred and seventy-ve shrimps with an average initial weight of 10.2 0.04 g were collected
and acclimatized for two weeks. Five experimental diets including control diet, 0.5% PA diet and 1%, 2%
and 4% ACV diets were applied to feed the shrimps. They were fed 4 times a day with 2.5% of body
weight. After 60 days of culture, shrimps fed with ACV and PA diets showed no signicant difference in
growth performance. Expression of prophenoloxidase (proPo), lysozyme (Lys), penaeidin-3a (Pen-3a)
and Crustin (Cru) genes were determined from hepatopancreas, using the real-time PCR after 15, 30 and
60 days. Expression of Lys and proPo genes was signicantly up regulated in shrimps fed with ACV and
PA diets compared to the control group after 30 and 60 days of treatment. After 15 days, Pen-3a gene
expression was signicantly higher in PA group compared to the control group. Also, shrimps fed with 1%
and 4% ACV and PA diets showed signicantly increased expression of Pen-3a after 30 days. In contrast,
expression of Cru was signicantly down regulated in response to ACV diets, but, Cru expression in
treated shrimps with PA diet was greater than the control group after 30 and 60 days. Overall, the results
provided evidence that ACV could be used as a natural immunostimulant for shrimps in order to adjust
and enhance expression of the immune related genes.
2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND
license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Litopenaeus vanname
Apple cider vinegar
Propionic acid
Growth performance
Immune genes
1. Introduction
The occurrence and spread of diseases in aquaculture industry
can signicantly restrict the productivity and as a result, farmers
extremely use antibiotics in order to prevent diseases. The wide use
of antibiotics can increase concerns due to their negative effects on
growth performance, resistant bacterial strains in host animals,
potential harms to human and the environment. So their usage has
been limited or banned in many countries. Also, A protective way
such as using vaccines has been investigated to reduce the outbreak
of diseases and mortality in aquaculture systems; however, it
cannot be utilized for all crustaceans because they don't have
specic immune systems [1]. That is why, identifying appropriate
* Corresponding author. Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, 49138-15739, Iran. Fax: 98
E-mail address: firstname.lastname@example.com (S. Pourmozaffar).
1050-4648/ 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
and reduces harmful bacteria in the host [5,11,12]. Oral consumption is the most common way to add organic acids. The Shrimps
(L. vannamei) fed with butyrate showed lower number of Vibrio sp.
in the intestine [9]. Also, total viable bacteria and Vibrio numbers in
the hepatopancreas tiger shrimps (Penaeus monodon) fed with
organic acids signicantly decreased [2]. Similarly, the benzoic acid
in piglets could reduce, the number of gram negative bacteria in
piglets [10]. Recently, in vitro assays have used organic acids to
evaluate the inhibitory effects on growth of Vibrio harveyi and the
results indicated that the minimum inhibitory concentrations of
acetic and propionic acids were 0.041% and 0.03%, respectively [13].
Because of these promising results, assessment of immune related
genes activity caused by products of organic acids has been deemed
At the present time, feed additives of natural origin, used in
natural medicine with a fractional afnity. ACV (Apple Cider Vinegar) is one of these natural substances known for hundred years
from renaissance. In fact, it was rstly used some 5000 years ago.
Hippocrates, prescribed the mixture of honey and apple cider
vinegar as a therapy for various diseases [14]. ACV is an acidic solution produced by fermenting apples. ACV counteracts injurious
bacteria that might be present in some foods and could aid digestion process [15]. In addition, it was recently shown that this substance hase a variety of pharmacological functions, (e.g.
antioxidant, antidiabetic, etc.), and also is able to decrease the
cholesterol level [16].
Despite the benecial effects of dietary organic acids supplementation in animal species, to the best of our knowledge, no
previous studies exists regarding the application of dietary ACV in
aquaculture. Therefore, the objectives of the present study performed to investigate dietary ACV and PA effects on growth parameters and expression of immune related genes in Litopenaeus
vannamei with initial body weight of 10 g.
diet, 1%, 2%, 4% ACV and 0.5% PA diets to ensure equal amounts of
gelatin in all experimental diets. The proximate composition of
diets have been described in the methodology as determined
following standard AOAC methods [18] and previously described in
da silva et al. [9]. The method of Kjeldahl based on nitrogen levels
was used to determine the crude protein. The crude ber was
analyzed by the sulfuric acid detergent method. The dry matter was
estimated by gravimetric analysis after drying in an oven at 100 C
for 24 h. The crude ash was calculated gravimetrically by burning,
in a mufe furnace, at 550 C for 6 h. Finally, the crude lipid was
analyzed based on ether extractison.
2.2. Experimental design
Three hundred and seventy-ve healthy individuals of
L. vannamei (10.2 0.04 g) obtained from the local commercial
shrimp farm in Gomishan, Golestan province, Iran. They were
transported to the aquaculture lab at Gorgan university of Agricultural Sciences and Natural Resources then acclimatized to the
experimental conditions for two weeks. After adaptation, the
shrimps were randomly distributed to fteen 400 L berglass tanks
at 20.27 1.21 ppt and 25.84 1.64 C. Water quality parameters
were measured daily. Shrimps were fed daily with the experimental diets. Also, they were fed at 2.5% of body weight with its
experimental diets at 07:00, 11:30, 15:30 and 22:00 during the
experimental period (60 days). Food Ingredients and composition
are presented in Table 1. At the end of acclimatization, the shrimps
were randomly distributed to ve groups and fed with the commercial diet (control group) while the other groups were fed with
different doses of ACV (containing 5% acetic acid) including 1%, 2%
and 4% and 0.5% liquid Propionic acid (Sigma Aldrich St. Louis, Mo,
USA, pure 99.5%).
2.3. Biometry and growth parameters assay
Fish meal
Soybean meal
Wheat our
Wheat gluten
Shrimp shell meal
Fish oil
Vitamin complex
Mineral complex
Proximate composition (g kg1 dry weight)
Dry matter
Crude protein
Crude lipid
Crude ber
Crude ash
based on the genes identied in the cDNA library (Table 2). Realtime PCR was carried out in IQ5 system (Bio Rad, USA) using 1x
SYBR Green PCR Master Mix (SYBR Biopars, GUASNR, Iran), 100 nM
of each forward and reverse specic primers, 10 ng of cDNA template and nuclease free water to nal volum of 20 ml were used and
all thermal prole of PCR reaction mixtures was done after Miandare et al. [3]. Table 2 reveals the primers used for amplication of
Lys, Cru, Pen-3a, proPo and b-actin. To validate the real-time PCR
primers, each genes specic primer pair was run in duplicate, along
a temperature gradiant (55e65 C) in the same plate. The PCR efciency and change in relative mRNA expression were calculated
based on the standard melting curve [21] analysis with dilution
series cDNA (including ve dilutions from 1/5 to 1/200). The PCR
efciency was to the following equation [22].
Table 2
Primers used for the real-time PCR assay of four immune related genes and one internal control b-actin gene of shrimp.
Accession number
Efciency (%)
Table 3
Growth performance of shrimp fed with control and ACV (Apple Cider Vinegar) and PA (Propionic acid) diets.
IBW (g)
FBW (g)
SGR (% day
10.21 0.045a
14.09 0.59a
37.964 5.9a
0.64 0.08a
3.01 0.5a
10.26 0.045a
14.10 0.55a
37.451 5.8a
0.63 0.08a
3.06 0.52a
10.15 0.05a
13.87 0.59a
36.667 5.53a
0.62 0.08a
3.12 0.51a
10.20 0.025a
13.52 1.03a
32.867 5.21a
0.56 0.15a
3.67 0.99a
10.19 0.02a
13.69 0.43a
34.33 4.53a
0.59 0.06a
3.30 0.4a
IBW, Initial body weight; FBW, Initial body weight; WG, weight gain; SGR, specic growth rate; FCR, feed conversion ratio. Values (mean SE) containing different superscripts
in same row denote signicant difference among the treatments (p < 0.05).
enzymes in stomach, pancreatic and intestinal [7]. But, the mechanism of this effect in enhancing growth performance is not known
yet. In contrast, our results demonstrated that the additives used in
this study do not affect the performance in the stage of rearing
evaluated (10e14 g). In the present study, the shrimps fed with
ACV supplemented diets were obviously more active when feed
was supplied compared to those fed with the control diet after
about 15 days onwards. However, there is not sufcient data on
using ACV in aquaculture industry. Similarly, previous studies
have illustrated that utilizing different organic acids blends did not
signicantly enhance growth of tilapia (Oreochromis sp) and
rainbow trout (Oncorhynchus mykiss) [11,17].
Shrimp diseases globally are one of the main limitations for
making sustainable progress in shrimp aquaculture industry; so
studies of shrimp immune related genes expressions has received
accumulative attentions. The results of the present study were
exclusively based on the expressions of selected genes from
hepatopancreas. Furthermore, previous studies, have indicated that
the hepatopancreas is a crucial organ in the immune system of
penaeid shrimp [24]. In this study, it has been clearly shown that
the different immune related genes were expressed at different
The crustaceans lack a highly developed specic immune system unlike the vertebrates and rely highly on non-specic cellular
and humoral factors. Humoral responses include the proPo activating system, phosphatase, Lys and antimicrobial peptides (AMPs)
such as Pen, Cru, anti-lipopolysaccharide factors (ALFs), etc. [25].
Lys was named by Fleming in 1922 for the rst time [26]. Lysozyme,
also recognized as muramidases is a well-known AMP and this
protein is involved in a broad range of defense mechanisms
including bacteriolysis, opsonization, immune response, and antimicrobial and antiviral activity [27]. The rst penaeidae Lys cDNA
was introduced in Litopenaeus vannamei [28]. The results revealed
that, shrimps fed with of ACV and PA showed an increased
expression level of Lys gene after 30 and 60 days after of treatments. Therefore, studies of enhancing of immune system of
shrimps arising by the organic acid compounds such as PA is scarce.
However, increase of Lys gene expression was as a result of
enhancement of innate immune protection against infectious
pathogens, environmental toxicants and stressors. In agreement
with our results, Miandare et al. [3] reported positive effect of dietary probiotic supplementation on Lys expression gene in white
shrimp. The expression of Lys was up-regulated in hepatopancreas
6 h post-injection lipopolysaccharide [29]. Park and Choi [30]
demonstrated that lysozyme activity was signicantly higher for
Nile tilapia (Oreochromis niloticus) fed the mistletoe (Viscum album
coloratum) supplemented diets as well signicant decrease in
mortality was observed after challenge with Aeromonas hydrophila
infection. However, the Lys also had wide range of antibacterial
activities against Gram positive and Gram negative shrimp pathogens such as Micrococcus lysodeikticus, V. harveyi, Edwardsiella
tarda, V. alginolyticus and V. parahemolyticus [26,31e33]. Viral
infection can result in 100% mortality of aquatic organisms within
supplemented with PrimaLac showed signicantly higher expression level of Pen-3a in comparison with the control group. Cell wall
component from multi-strain probiotic (PrimaLac) such as lipopolysaccharides and peptidoglycans stimulate expression level of
Pen-3a in shrimp. Conversely, injection of lipopolysaccharide
inhibited the expression of Pen level in white shrimp [4]. Also,
white shrimp fed with b-1,3-glucan diets down-regulated Pen-3
gene after 6 and 12 h feeding [44]. Pen-3a might be a powerful
antibacterial protein which plays a role in innate immunity in
penaeid shrimp. An et al. [40] reported that MjPen-II demonstrated
antibacterial activity against both Gram positive and Gram negative
bacteria in kuruma shrimp (Marsupenaeus japonicus). On other
study suggested that, Pen 3 mRNA expression was up regulated
after microbial challenge with Vibrio parahemolyticus [41]. The upregulation of penaeidin mRNA expression increases young haemocytes which are actively expressing penaeidin genes in the
circulating haemocyte population, resulting in an enhancing
phagocytosis and agglutination [4].
The proPo system is a strong regulated defense mechanism that
is involved in biosynthesis of melanin and production of toxic intermediates for pathogenic [45]. Also, proPO was rst introduced in
the freshwater craysh Pacifastacus leniusculus in 1995 [46]. The
prophenoloxidase activating system (proPOAS) is an efcient non
self-recognition system which consists of several important proteins including proPo, pattern-recognition proteins (PRPs), serine
protease (SP), and serine protease inhibitors (serpins). The proPO, is
inactive proenzyme of phenoloxidase (PO) [47]. According to the
other investigations two types proPo genes was cloned and
described from the species L. vannamei and Penaeus monodon
[48,49]. Relative expression of proPo, signicantly increased in
ACV and PA treatments during the treatment period. The proPO
activity response to immune stimulants such as, probiotic (PrimaLac and Lactobacillus plantarum), lipopolysacharide and Sargassum
wightii has previously been reported in L. vannamei and Penaeus
monodon [3,4,46,50]. These results demonstrated that dietary ACV
and PA administration could enhance PO activity resulting from
advancement the gene transcription and translation of proPOs in
L. vannamei. In agreement with our results, some researchers has
shown that, shrimps fed with the diet containing organic acids
blend showed enhanced PO activity and consequently led to higher
survival against Vibrio harveyi [2,5]. Amparyup et al. [48] and Chiu
et al. [46] reported up regulation of proPo expression increased
resistance to Vibrio alginolyticus. Furthermore, another study reported up regulation of proPo in shrimp fed with Bacillus subtilis
diets compared to the control group after challenge with V. harveyi
[20]. In contrast, based on some investigations proPo expression
was down regulated in shrimps challenged with white spot syndrome virus (WSSV) [51,52] and necrotizing hepatopancreatitis
bacterium after 24 days [53]. Similarly, ProPo expression was down
regulated and caused raised Penaeus monodon mortality following
V. harveyi infection [54]. So, the expression and regulation of proPo
of shrimps were signicantly related to the diet administration,
environmental changes and pathogens challenge. Previous studies
demonstrated that organic acids led to improved resistance of
shrimp to pathogens [2,5,11]. This led to the hypothesis that they
contribute to regulate the AMPs secration of shrimp and maintained or increased the immune response of shrimp. However, little
information is available about the molecular mechanisms of
organic acid on immune related genes of shrimp.
5. Conclusion
The present study revealed that ACV and PA diets had no
benecial effects on growth performance in the stage of rearing
evaluated in this study (10e14 g). Furthermore, transcriptomic
zbir, Apple
[16] M. Nazirolu, M. Gler, C. Ozgl,
G. Saydam, M. Kkayaz, E. So
cider vinegar modulates serum lipid prole, erythrocyte, kidney, and liver
membrane oxidative stress in ovariectomized mice fed high cholesterol,
J. Membr. Biol. 247 (2014) 667e673, http://dx.doi.org/10.1007/s00232-0149685-5.
[17] Z. Xie, F. Wang, H. Liu, S. Guo, A. Zhu, H. Niu, Gelatin-walled microencapsulated diet for larval shrimp (Penaeus japonicus Bate) manufactured
using the uidized bed coating process, Aquac. Res. 42 (2010) 65e73, http://
[18] AOAC, AOAC Ofcial Methods of Analysis, Association of Ofcial Agricultural
Chemists, Washington, D.C., 1992, pp. 136e138, 15th.
[20] H. Zokaeifar, J.L. Balcazar, C.R. Saad, M.S. Kamarudin, K. Sijam, A. Arshad,
N. Nejat, Effects of Bacillus subtilis on the growth performance, digestive
enzymes, immune gene expression and disease resistance of white shrimp,
Litopenaeus vannamei, Fish Shellsh Immunol. 33 (2012) 683e689, http://
[21] A. Larionov, A. Krause, W. Miller, A standard curve based method for relative
real time PCR data processing, BMC Bioinforma. 6 (2005) 62, http://dx.doi.org/
[22] A. Radonic, S. Thulke, I.M. Mackay, O. Landt, W. Siegert, A. Nitsche, Guideline
to reference gene selection for quantitative real-time PCR, Biochem. Biophysical Res. Commun. 313 (2004) 856e862, http://dx.doi.org/10.1016/
[24] D. Pan, N. He, Z. Yang, H. Liu, X. Xu, Differential gene expression prole in
hepatopancreas of WSSV-resistant shrimp (Penaeus japonicus) by suppression
subtractive hybridization, Dev. Comp. Immunol. 29 (2005) 103e112, http://
[25] D. Banerjee, B. Maiti, S.K. Girisha, M.N. Venugopal, I. Karunasagar, A crustin
isoform from black tiger shrimp, Penaeus monodon exhibits broad spectrum
anti-bacterial activity, Aquac. Rep. 2 (2015) 106e111, http://dx.doi.org/
[26] Y. Tian, X. wang Liang, Y. qing Chang, J. Song, Expression of c-type lysozyme
gene in sea cucumber (Apostichopus japonicus) is highly regulated and time
dependent after salt stress, Comp. Biochem. Physiology Part - B Biochem. Mol.
Biol. 180 (2015) 68e78, http://dx.doi.org/10.1016/j.cbpb.2014.10.004.
[27] W. jun Mai, W. na Wang, Protection of blue shrimp (Litopenaeus stylirostris)
against the against WSSV challenge (WSSV) when injected with shrimp
lysozyme, Fish Shellsh Immunol. 28 (2010) 727e733, http://dx.doi.org/
[28] T. Chen, C. Ren, Y. Wang, P. Luo, X. Jiang, W. Huang, C. Chen, C. Hu, Molecular
cloning, inducible expression and antibacterial analysis of a novel i-type
lysozyme (lyz-i2) in Pacic white shrimp, Litopenaeus vannamei, Fish Shellsh
Immunol. 54 (2016) 197e203, http://dx.doi.org/10.1016/j.fsi.2016.04.008.
[29] A.B. Peregrino-Uriarte, A.T. Muhlia-Almazan, A.A. Arvizu-Flores, G. GomezAnduro, T. Gollas-Galvan, G. Yepiz-Plascencia, R.R. Sotelo-Mundo, Shrimp
invertebrate lysozyme i-lyz: gene structure, molecular model and response of
c and i lysozymes to lipopolysaccharide (LPS), Fish Shellsh Immunol. 32
(2012) 230e236, http://dx.doi.org/10.1016/j.fsi.2011.10.026.
[30] K.H. Park, S.H. Choi, The effect of mistletoe, Viscum album coloratum, extract
on innate immune response of Nile tilapia (Oreochromis niloticus), Fish
Shellsh Immunol. 32 (2012) 1016e1021, http://dx.doi.org/10.1016/
[31] W. Mai, C. Hu, cDNA cloning, expression and antibacterial activity of lysozyme
C in the blue shrimp (Litopenaeus stylirostris), Prog. Nat. Sci. 19 (2009)
837e844, http://dx.doi.org/10.1016/j.pnsc.2008.10.008.
[32] E. De-La-Re-Vega, A. Garca-Galaz, M.E. Daz-Cinco, R.R. Sotelo-Mundo, White
shrimp (Litopenaeus vannamei) recombinant lysozyme has antibacterial activity against Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae, Fish Shellsh Immunol. 20 (2006) 405e408,
[33] H. Li, M.G. Parisi, M. Toubiana, M. Cammarata, P. Roch, Lysozyme gene
expression and hemocyte behaviour in the Mediterranean mussel, Mytilus
galloprovincialis, after injection of various bacteria or temperature stresses,
Fish Shellsh Immunol. 25 (2008) 143e152, http://dx.doi.org/10.1016/
[34] C. Sun, X.J. Du, W.T. Xu, H.W. Zhang, X.F. Zhao, J.X. Wang, Molecular cloning
and characterization of three crustins from the Chinese white shrimp, Fenneropenaeus chinensis, Fish Shellsh Immunol. 28 (2010) 517e524, http://
[35] P. Supungul, S. Tang, C. Maneeruttanarungroj, V. Rimphanitchayakit, I. Hirono,
T. Aoki, A. Tassanakajon, Cloning, expression and antimicrobial activity of
crustinPm1, a major isoform of crustin, from the black tiger shrimp Penaeus
monodon, Dev. Comp. Immunol. 32 (2008) 61e70, http://dx.doi.org/10.1016/
[36] W. Rattanavichai, Y.N. Chen, C.C. Chang, W. Cheng, The effect of banana (Musa
acuminata) peels hot-water extract on the immunity and resistance of giant
freshwater prawn, Macrobrachium rosenbergii via dietary administration for a
long term: activity and gene transcription, Fish Shellsh Immunol. 46 (2015)
378e386, http://dx.doi.org/10.1016/j.fsi.2015.06.031.
[37] A. Rattanachai, I. Hirono, T. Ohira, Y. Takahashi, T. Aoki, Cloning of kuruma
prawn Marsupenaeus japonicus crustin-like peptide cDNA and analysis of its
expression, Fish. Sci. 70 (2004) 765e771, http://dx.doi.org/10.1111/j.14442906.2004.00869.x.
[38] S.G. Hipolito, A. Shitara, H. Kondo, I. Hirono, Role of Marsupenaeus japonicus
crustin-like peptide against Vibrio penaeicida and white spot syndrome virus
infection, Dev. Comp. Immunol. 46 (2014) 461e469, http://dx.doi.org/
[39] J.E. Shockey, N.A. O'Leary, E. de la Vega, C.L. Browdy, J.E. Baatz, P.S. Gross, The
role of crustins in Litopenaeus vannamei in response to infection with shrimp
pathogens: an in vivo approach, Dev. Comp. Immunol. 33 (2009) 668e673,
and its role in shrimp immune responses against major pathogens, Fish
Shellsh Immunol. 34 (2013) 990e1001, http://dx.doi.org/10.1016/
M.S. Yeh, C.Y. Lai, C.H. Liu, C.M. Kuo, W. Cheng, A second proPO present in
white shrimp Litopenaeus vannamei and expression of the proPOs during a
Vibrio alginolyticus injection, molt stage, and oral sodium alginate ingestion,
Fish Shellsh Immunol. 26 (2009) 49e55, http://dx.doi.org/10.1016/
M. Sivagnanavelmurugan, B.J. Thaddaeus, A. Palavesam, G. Immanuel, Dietary
effect of Sargassum wightii fucoidan to enhance growth, prophenoloxidase
gene expression of Penaeus monodon and immune resistance to Vibrio parahaemolyticus, Fish Shellsh Immunol. 39 (2014) 439e449, http://dx.doi.org/
H.S. Ai, J.X. Liao, X. De Huang, Z.X. Yin, S.P. Weng, Z.Y. Zhao, S.D. Li, X.Q. Yu,
J.G. He, A novel prophenoloxidase 2 exists in shrimp hemocytes, Dev. Comp.
Immunol. 33 (2009) 59e68, http://dx.doi.org/10.1016/j.dci.2008.07.017.
H.S. Ai, Y.C. Huang, S.D. Li, S.P. Weng, X.Q. Yu, J.G. He, Characterization of a
prophenoloxidase from hemocytes of the shrimp Litopenaeus vannamei that is
down-regulated by white spot syndrome virus, Fish Shellsh Immunol. 25
(2008) 28e39, http://dx.doi.org/10.1016/j.fsi.2007.12.002.
M.D. Figueroa-Pizano, A.B. Peregrino-Uriarte, G. Yepiz-Plascencia,
n, L.R. Martnez-Co
rdova, Gene expresM. Martnez-Porchas, T. Gollas-Galva
sion responses of white shrimp (Litopenaeus vannamei) infected with necrotizing hepatopancreatitis bacterium, Aquaculture 420e421 (2014) 165e170,
W. Charoensapsri, P. Amparyup, I. Hirono, T. Aoki, A. Tassanakajon, Gene
silencing of a prophenoloxidase activating enzyme in the shrimp, Penaeus
monodon, increases susceptibility to Vibrio harveyi infection, Dev. Comp.
Immunol. 33 (2009) 811e820, http://dx.doi.org/10.1016/j.dci.2009.01.006.


text:
"Narcissa...?" Lucius says his words going to deaf ears only adding to his worry.
Every breath left Narcissa when Zale told her that she would not see Topaz anymore and that she will forget her completely. Can that even happen? Memory can be erased but feelings too? Narcissa though.
Lucius put an arm around Narcissa waist to get her to walk because she currently was blank like a dementor gave her a kiss which no doubt was like she felt right now.
"Sirius, take Corvus back the villa," Lucius says
"I don't need to be told twice," Sirius grumbled
"And Sev-"
"Don't waste your breath Lucius. I'll find everything I can but I make no promises," Severus says before he apparates to who knows where.
"Let me," Tom says coming over side of Narcissa and helping Lucius who opens his mouth to protest about him being able to take care of Narcissa which Tom beats him too it.
"Before you say anything I do want to remind you that might be the dark lord but I still have heart," Tom says his words drawing a little blush to Lucius cheeks, which he shook off as they made their way back to the villa.
Bellatrix waited for them at the entrance her hand flying to her mouth to cover her shook at the sight of her sister who looked more like a corpse.
"What happened to her?" Bellatrix asks after leading them to one of the bedrooms so they can lay Narcissa on it.
Lucius told her what happened; Bellatrix shook her head in disbelief.
"How could she do this to Cissy!" Bellatrix says magic cracking around her that one glare from Tom had it disappear.
"Did you even listen to Lucius? Neither, Topaz or Narcissa wanted to that but for Corvus sake Topaz did it and Narcissa even agreed however, none of us expected this," Tom says
-With Draco and Corvus-
Corvus wakes up his body relaxed and without that pressure of something inside of him trying to boil over. Corvus sits up slowly not wanting to overdo it. His movement catching Draco's attention, "I'm glad you're okay," Draco says a tired smile on his face, eyes slightly red as if he had been crying but Corvus knew best than to ask if he did. So instead he asks, "Did something happen?"
Draco nods and tell him what happened to his mother and that Topaz was not going to return.
Corvus shook his head, "That's not true!" Corvus says
Draco furrows his brows in question, "What you mean?" he asks
"Right after Topaz and some guy helped me she embraced me and whispered 'I'll be back soon so be safe still then.'" Corvus says
-With Topaz-
Topaz slapped Zale hand away, "I don't need help swimming," Topaz said a bite to her words
Zale shrugs and says, "Just wanted to help,"
Topaz blue eyes got darker in anger, "You helped enough!" Topaz says increasing her speed in which they soon reached a border of a cliff and right below it was a city. It was breath-taking view from its unique architecture of stone, shells and other materials. Nevertheless, Topaz was not in a mood to enjoy the view of her home but only swam straight to the biggest building at the north belonging to the royal family. Also the place where her father was.
Topaz went straight to the throne room where her father could be found often at, everyone around her stayed out of her way from the furious expression on her face.
Her father looked similar to Zale only older, bigger and a sad look on his face, which seemed to turn permanent since her mother was killed.
His gaze lit up when he saw Topaz making her way to him and he shooed away the fish that was giving him a report. To give his full attention to his oldest daughter.
"Topaz, how I missed you," he says getting up from his throne and moving to Topaz who was having a hard time being angry and it didn't help when she was engulfed in his big arms and strong chest.
That she could not stop her tears from dropping down as she clung to him.
They stayed like that for a few minutes and he pulled back using one of his big fingers to wipe away his daughter tear.
"Please tell me why you left was something I did, your siblings or someone else?" he asks
Topaz just moved away from him not looking at him when she answers, "I went to look for my mate..."
He stopped listening to her when she said the first line his face turning into anger.
"WHAT! You-" he started
However, Topaz easy cut him off, "I heard it multiple times dad and I understand your feelings but that's no reason for you to let it cloud your judgement! I understand that you want to keep us safe but at what cost dad?"
His anger lessened slightly most of it shook from being talked to like that. Topaz did not even let him talk and she continued saying, "I never did anything to give you trouble so why not allow me to be happy. You were happy with mom and"
The sound echoed around them, Topaz raised her hand to her red cheek that burned from the intensity of his slap.
"Yyyou never hit me before..." Topaz trailed off
Her face twitching from the pain. He lowered his hand trembling hand he could not believe that he just did that. He never hit none of his children but what Topaz said had his body acting on its own accord.
"Topaz, I'm sorry," he says moving closer to her but she just shook her head and moved away from him.
"No dad you're not sorry. You wanted to show that you're in charge and that I should listen but I will not because you make it seem as if you are hurting the most from mom's death. Which you are wrong I hurt more because I was there when it happened and it was my fault that she was killed." Topaz says
"Sweetheart it was not-"
Topaz shakes her head and says, "It was my fault dad you might not see it that way but it was because I wanted to see my mate instead of listening to mom when she said to wait and think about it. However, my desire to have something like you and her had got the best of me so if you want to blame someone for her death blame me. Because if I did not went back she would still be alive and it was not their fault either because they did not know what I was not going to harm their child who was my mate. They only acted like a parent would and protected their child like mom did to me,"
Topaz turns to leave only to be stopped by his voice, which sounded pained and sad, "Your room is still the same,"
"Yeah," Topaz says.
Underwater Friend (Harry Potter FanFiction)Fanfiction
Harry meets a siren while hiding from his cousin and his goons. The siren makes him question things about himself and the people around him. Which gets him to rethink on who he should really trust along with helping the siren remove the hate from h...


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Apr 16 2019
Heavy rain from the thunderstorms may lead to isolated instances of flooding and a "short-lived tornado" is also possible.
The weather service issued numerous thunderstorm and tornado warnings between 1 and 2 a.m. today for the Southern Tier and Catskills. Mobile homes will be damaged or destroyed.
More than 20,000 customers lost power in New Jersey when the storms hit, though crews were able to quickly restore service to most.
Two children, ages 3 and 8, were killed in eastern Texas when a tree fell on the vehicle in which they were traveling.
As the storm moved east, one person was killed by a tornado in the northern MS, according to Monroe County Coroner Alan Gurley.
If a tornado warning is issued in your area, the National Weather Service says to immediately take shelter in a basement or lower-level room away from windows.
A local group is heading to Alto and Franklin to help those impacted by a tornado and severe storms that swept through the state Saturday, killing two children. At least 11 tornadoes have touched down in three different states as a result of a stronger-than-usual storm system.
The roof of a hotel in New Albany, Mississippi, was damaged, although the cause was unclear.
Robertson County Sheriff Gerald Yezak told The Associated Press a tornado hit the small Central Texas city of Franklin, overturning mobile homes and damaging other residences.
In East Texas, authorities say two children were killed when high winds toppled a tree onto the back of the family auto while it was in motion. Franklin is about 125 miles south of Dallas. The parents in the front seats were not hurt.
Winds reached 60 miles per hour in Binghamton, the weather service said, as lines of severe thunderstorms rolled into the Northeast Sunday night.
"It looks like a storm came up through Louisiana and into MS and exploded in Monroe County", Monroe County Sheriff Cecil Cantrell told CNN.
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News on April 2017
First Bookgroup meetings of the term
28 April, 2017
On Monday 24 April the Sixth Form Book Group met to discuss The Sellout by Paul Beatty. Here are some of the members' thoughts about this book… This book started [...]
The Future is looking very bright for our Upper Sixth!
27 April, 2017
When boys leave a school like Merchiston, we wish them to be in the best possible position for the next stage of their life. If they decide to go to [...]
Headmaster's Headlines
25 April, 2017
This morning at Headmaster's Assembly we sang Happy Birthday to Robbie and Oscar. Many happy returns! Thoughts I always like to present to the School a different speaker epitomising different [...]
D of E: Gold Award practice
25 April, 2017
The Gold Duke of Edinburgh group consists of seven boys, six of whom have already completed their Bronze and Silver awards in a variety of different countries. This experience showed [...]
Sri Lanka – Foundation of Goodness donation
24 April, 2017
15 very fortunate boys have recently returned from Sri Lanka for a 12 day tour of the country. They visited Kandy, Hikkaduwa, Galle and Colombo playing five matches against local [...]
Commemoration Sunday, 23 April 2017
24 April, 2017
Following the new pattern established last year, the annual Commemoration service took place on the first Sunday of the Summer Term, and it was a real pleasure to welcome back [...]
Golf Academy news
20 April, 2017
On Wednesday 19 April, Merchiston Golf Academy pupils played for The Scottish Wayfarers' team against the Royal and Ancient Golf Club of St Andrews. The match was played over the [...]
Headmaster's Headlines
19 April, 2017
Thoughts First, I spoke to the School about the joy of the Summer Term. Of course, so many readers of Headmaster's Headlines are from Scotland, but to this day, I [...]
D of E Silver Award Biking Practice
19 April, 2017
With one participant fewer than expected, it was a quick reshuffle of tents and a speedy visit to the supermarket for some alternative provisions. We made good time to Aberfoyle, [...]
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RT @UKboarding: Great session on 'What does good look like?' and the importance of brand last week with @MerchiNews - talking of brand, her…
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The Games! Good luck to all boys competing in this wonderful day of Athletics today. All information and races onli… https://t.co/czifA0Ldi3
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🏏 💥 2019 Clan Cricket Winners - Dreghorn 🏏 💥 https://t.co/62wu9cKDLf
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Reuben F hits the winning boundary off the penultimate ball! Congratulations to Dreghorn on the victory. What a gam… https://t.co/MZyvPhud5j
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A blustery morning for #hillrunning #@Merchi6th https://t.co/pDfiYbz4WP
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Cricket Teams returning from Ardvreck ETA delayed 19:00 🏏
@Merchi_CE09 Nov
Social at St George's tonight followed by Scotland v Fiji on Saturday - busy weekend for the boys from East.
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Clan cricket finals and BBQ @Merchi_CE @MerchiNews @BSAboarding #iloveboarding #nationalboardingweek https://t.co/kM5ybaf7wL
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...and in the middle of his GCSEs! Great work Tom! https://t.co/IlGc2yL0jh


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If you are new to diagnostic scanners, this handy tool connects to your vehicle, scans various parts of your car, truck or suv, and retrieves a list of diagnostic trouble codes dtcs and code definitions from your vehicles computer. Multilingual menu and options — english,. Depending on the Drive Cycle Monitors one of these 2 screens will be present. So far, I highly recommend this unit. Upgraded features include the ability to read enhanced codes, graph engine data, record and playback stored vehicle information, and the ability to print information through a pc. Toggle the Field Service mode on and off.
I happen to be an automotive technician and I have to say that some of these are great, but when it comes down to it I'll take a real piece of diagnostic equipment any day. The proportion of air and fuel delivered to the cylinder for combustion. Select desired vehicle to diagnose. Ultimately, it is our pleasure to help you work less and live more. All makes and models with detachable rubber hose. Based on the vehicle information entered at the Vehicle Setup menu, the Scan Tool recognizes the computer system installed. Perform the following: 1 Set parking brake.
Tester works on: All makes and models with schrader valve test port on fuel rail. Therefore, the maximum number of frames that can be recorded varies. Easy-to-view digital display shows numeric trouble codes - eliminates the need to count flashes. It has the ability to produce graph data, tests, live sensor data, and state emission recordings. Obviously the guy who wrote this useless article doesn't have a clue.
A stored code points in the direction the problem appears to be. Based on the vehicle information entered at the Vehicle Selection menu, the Scan Tool automatically recognizes the computer system installed. . A relatively simple act such as jump starting a car, changing a fuse or disconnecting the battery for charging, is enough to generate potentially crippling spikes. Any system where a computer controls fuel delivery to the engine by using fuel injectors.
Return to Diagnostic Codes Menu. Intuitive interfaces and numerous support services remove complexity and ambiguity from vehicle repairs. This function is mainly used for diag- nosing intermittent driveability problems that cannot be isolated by any other method. It arrived intact as promised with all the attachments, Win 7 dvd,printed instructions, and a case. That helps to save money by not throwing parts at a vehicle. Look for items sold by Walmart. Perform the following: 1 Turn ignition key off.
The major reason why it is so prominent is because of its features and the manner in which it functions. This scanner makes sure the light is gone and removed. Had a problem with the driver but, called Actron support and the girl help me through it. Close all programs on the computer. Plugged in the Actron and it found it. Cp9190 kit includes cp9185 90 base toolobd ii cablegm obd I aldl cableford obd I mcu eec iv cablechrysler obd I sci cableusb cable8 foot extension cablecigarette lighter adapterblow molded hard casemanual cdregistration card. Going to try to see if Amazon or Actron will step up to the table to tell me what's happening to this unit or replace it.
This makes sure the individual that is using the tool is able to get an immediate diagnostic. The snap on of course does much more. In the meantime, though, I returned it as it doesn't address my current need. All actron code readers and scan tools are can compliant. Durability- the scan machine has a casing where it is kept. Regardless of how new the individual is to these scan tools, it is easy to use for auto repair purposes. The port is there so anyone can hook up to it and do diagnostics.
Instead of having to recreate a scenario over and over again, you can now playback the timing to scrutinize all the data and make a clear diagnosis. I answered them and thanked him as well. We are still learning how to use this scanner with all of its features. How many minutes in a few?!?!?! This device not only scans your vehicle, it also allows you to freeze frame live timing and pinpoint your vehicles problem. I go into it, I see View Freeze Data, enter into that and see the P0446 code and reported fix code connect worked. Can I recommend its use? What else does the menu have to offer? Sees everything and reports all.


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He Kept a Stump On Their Lawn. 14 Days Later His Wife Removes The Bag and Reveals THIS
sun-gazing.com - 03-12
It's always sad when an old tree dies on my property, that needs to be cut down…and the left-over stump can be an eyesore. Mimi and her husband faced this unsightly stump right in their front yard, after her husband cut down the dead tree.


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Kimberly Nelson
CGD218: Visual Literacy in Business
Rob Shah
November 26, 2013
There are many different reason Indi vials abuse animals. Animal cruelty covers a wide range of actions. Each type of abuse has displayed certain patterns of behavior that we can use to help understand more about why people commit crimes we encounter today. Animals are often broken down into two main categories': active and passive, also referred to as commission and omimission. Passive cruelty is typified by causes of neglect, where the crime is a lack of action rather than the action itself-however does not let the terminology fool you. Severe animal neglect can cause incredible pain and suffering to an animal. Examples of this neglect are starvation, dehydration, parasite infestation, allowing a collar to grow into an animal's skin, inadequate shelter in extreme weather conditions, and failure to seek veterinary care when animal needs medical attention. In many cases of neglect where and investigator feels that cruelty occurred as a result of ignorance, they may come in and reeducate the owner giving them a second chance to prove they can take care of the animal. If failure to do so this may leave them no choice to take the neglected animal away and give them the shelter they are needed, and you may or whomever to face crimal charges and possible prison term with the laws that are in effect for animals today. Active cruelty implies malicious intent, where a person deliberately and intentionally causes harm to an animal, and is sometimes referred to as NAI (Non-accidental Injury). Acts of intentional cruelty are often some of the most disterburing and should be considered signs of physiological problems. This type of behavior is often associated with sociopathic behavior and should be taken seriously. In one of the pictures it shows a kitty with one eye caused by animal abuse and I want to share the story of it with you. This is a true story that happened right here in the state of Wisconsin. The story is called rescued Kitten trapped in pickup truck engine. (Found on ASPCA). Bob is a manager at a big, busy, modern hardware store in Southwest Wisconsin; one warm day not long ago a customer raced into the store and excitedly told him that there was a sound of a kitten yowling loudly inside a pickup truck. Bob did not hesitate, he ran out to invest age. He heard the frantic kitten too- sounds coming from inside of a wheel well, but Bob could not see the kitten. Bob hurried back to the store. He told a staffer to call the humane society and the police. He asked another to try to find the owner of the vehicle. Then he grabbed his tools and hurried back to the truck. He crawled under the truck and got to work he found the kitten. Now to get it out safely. Bob freed the kitten and handed the little wiggly critter up to animal control officer. There was a TV crew to catch Bob in his courageous act to save the kitten. The kitten went to the humane society for exanimation and care and later was adopted. The moral of this true story is that this kitten could have died because of someone's stupidity and it would have been considered animal abuse.
The first step of animal abuse is to evaluate that an animal is being neglected is a collar may be too tight which can cause injury, strangulation, and can cause death. Lack of grooming without regular grooming, a pet, especially a long haired can get massive matting and sores. Mange which causes by tiny parasites, which lead to…


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John Salako: I thought England call-up was a Richard Shaw prank1 June 2019
In mid-1991, John Salako was out mowing the lawn when a phone call came that every Englishman dreams of.
The long read: How Hodgson saved Swiss football31 May 2019
Roy Hodgson managed the Switzerland national side between 1992-95, and in that time, he pulled off one of the most remarkable achievements seen in international football. 24 years later, with Crystal...
Neil Shipperley - The West Ham play-off final was my proudest moment29 May 2019
15 years ago to the day, Crystal Palace launched themselves into the Premier League and memories of one match from 2004 - a distant past given Palace's packed history since - will stand out strongly...
Mark Butcher previews England's huge summer of cricket, and talks Palace29 May 2019
Mark Butcher, fondly known as Butch, hung up his gloves a decade ago at his home ground The Oval, bringing a spirited cricket career to a close. On the eve of England's opening game of the ICC Cricket...
Catching up with Kieron Cadogan: Edgar Davids, Jules, AWB and plenty more20 May 2019
In two months' time it will have been seven years since Kieron Cadogan departed Crystal Palace, another south London and proud local lad who made a name for himself in the beautiful game.
Sticky business: Strangest Palace stickers revealed...19 May 2019
Last season, the Crystal Palace programme delved into the history (sticker)books to pluck out some of the strangest and most eye-catching of football stickers with a Palace star featured. Below, we've...
Ray Lewington offers explanation for Palace's difference in home and away form16 May 2019
At the end to a successful season, Crystal Palace entertained fans with a remarkable 5-3 home dismantling of AFC Bournemouth.
Smashed it: Personal bests posted by several Eagles for the 2018/19 campaign16 May 2019
What an entertaining finish Roy Hodgson's side put on for the final game of the 2018/19 campaign for the Eagles.
Read Julian Speroni's farewell message in full15 May 2019
Julian Speroni offered an emotional goodbye to Selhurst Park after watching Crystal Palace defeat AFC Bournemouth 5-3 in his last ever visit as a Palace player. You can read what he managed to say in...
Hodgson: This is Selhurst Park15 May 2019
As season endings go, Crystal Palace's close to the 2018/19 campaign was up there with the most entertaining and emotional.
Scott Guyett reveals roommate connection helped Palace sign Joel Ward9 May 2019
"He's got a lot to thank me for, put it that way!", jokes Scott Guyett in Crystal Palace's media centre, just across the car park from his usual office as Head of Sport Science and Strength &...
Speroni's iconic Palace career in numbers9 May 2019
Julian Speroni is now coming to the end of an iconic career representing Crystal Palace over nearly 15 years. Here, the official Palace programme reflects on the shot-stopper's remarkable time in...
Zaha: 'Speroni is a Palace hero for sure'9 May 2019
Having spent over nine years playing alongside one another professionally, Wilfried Zaha and Julian Speroni have written their name into Palace history side by side, each becoming a south London...
Parish: 'Our supporters are just unbelievable'4 May 2019
Speaking at Crystal Palace's End of Season Awards on Tuesday evening, Palace chairman Steve Parish expressed his pride and admiration for the red and blue faithful, describing fans as 'unbelievable'.
Darren Powell reveals the U15s to watch before cup final v Wigan tonight3 May 2019
Crystal Palace have sent an Under-15 squad to Walsall where they will face the biggest clash of their season as they vie to win the National Floodlight Cup final against a strong Wigan Athletic outfit...
Ciara Watling: Palace fans are 'best in the league'28 April 2019
"Their support, their singing, their songs for us - most women's teams don't actually get that," argues Ciara Watling.
James McArthur full of praise for 'brilliant man' Hodgson26 April 2019
James McArthur sang the praises of Crystal Palace manager Roy Hodgson when speaking with Premier League Productions earlier today, describing him as a 'brilliant man'.
Guaita reveals inspiration behind eye-catching hair and why he 'thanks' the posts18 April 2019
Vicente Guaita is hard to miss in south London.
PC Leon McLeod: On the frontline of London Bridge terror attack15 April 2019
How many days does it take you to return to work after finding yourself at the epicentre of a terror attack?
Zaha on his strengths and weaknesses and the one question he always gets asked!13 April 2019
After the final training session ahead of an exciting game today against Manchester City, Wilfried Zaha spoke with Premier League Productions in a wonderfully open interview.
Hodgson explains how Palace overcame Manchester City13 April 2019
Roy Hodgson spoke in his weekly press conference earlier in the week to preview Crystal Palace's home clash with Manchester City, seeming like a positive figure in the face of one of the league's most...
Hodgson: Luka reminds me of one former player of mine12 April 2019
Roy Hodgson was in confident mood ahead of this weekend's clash with Man City, a game in which Luka Milivojević could equal Andrew Johnson's Premier League record of 11 penalties scored in one season,...
Martin Kelly reveals all about the Crystal Palace Fantasy Football dressing room chat12 April 2019
Just like millions around the world, Martin Kelly revealed that the Palace dressing room and group chats are full of Fantasy Football chat. But who is rivaling Kelly for highest-ranked first-teamer?
Behind the creation of Palace fans' mental health awareness walk10 April 2019
"I don't want anyone to feel like I ever did," Paul Price says with a remarkably positive tone.
Penalty King Milivojevic reveals his spot kick secrets10 April 2019
Luka Milivojevic's penalty-taking heroics are no secret to anyone. However, what hasn't been common knowledge before were the steps Palace's captain takes to make himself one of the finest craftsman...


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And as always, if you like what you see, please help others find it by sharing it. The game is autosaving, so you can continue to play whenever you want. Then tap the plants on the left a few times to move them. You fell asleep and you find yourself locked inside a house which you have never seen before but looks lively. But no worries you can always visit us and find all the Can You Escape The 50 Rooms 2 walkthrough you need. Now you can open the door.
Sniffmouse PictureIt is another picture puzzle game developed by for. Shake, swipe, flip your phone, and solve puzzles. No wifi required When you are stuck, three kinds of hints will help you 240+ levels without repeat, be a master of training brain! One day, the boy became stuck in a house by mistake. Guess the door codes and word solutions. This page will let you access all of the most recent new developments and announcement. Or as new levels get released. I t will help you to find the hidden clues in the form of colours and numbers and to locate and use the items in the environment.
A game created by Worzzle Team a developer of many popular games like Wordscapes and Words Story Games. Let us know in the comments if you like it or hate it. Door 2: Tilt your phone left to roll the ball and the door will open. For technical support about any game, you can contact the developer via Play Store. Escape Room Mystery Word Levels 51-60 Answers.
Every time you interact with our cat, you are raising money for animal charities. When you are confused,Humanized hints,Help you to escape. Door 3: Tilt your phone left. There was a very large and beautiful city. So feel free to share this post with your those friends that you know are playing the game. Slip it in the hole and then turn it to open the door.
There are thousands of addicting in our archive. You Will find in this topic the answers of Escape Room Level 1 to 100, the game is developed by Worrzle Team and its aim is to fulfill the blank tiles with words available at the tray and using the available hint. Pull the handles from right to left and back. There is no time limit nor an internet connectivity is required, so you can even play it offline. Use items on specific spots to complete puzzles. It took us quite a while to solve, in fact I got stuck a few times. Your duty is to rescue the girl from there.
Good luck and have fun! Good luck and have fun! To find the solution of the game and to get out from the rooms you must solve different riddles and puzzles. There was a huge fort in the middle of a beautiful magnificent forest. Use any possible way to open door like drag, shake, touch the floor, swipe, tap ,tilt ,push buttons and so on. Pull the handles from right to left and back. If you like what you see on AppUnwrapper. Select it from your inventory and use it to slide the door left note the arrow above the door. For technical support about any game, you can contact the developer via Play Store.
I am updating all levels as some of you requested me to do it. You can also select and play weekly and monthly from our right sidebar. Good Luck and have fun! You need to time it right so the circular cutouts in each door hit the green area at the same time. Escape Room Level 1 to 100 Answers : Features: Simple, easy rules, type the correct word to win a game! Although Jon had been ill for a long time, it was still a shock. Find them all and solve all puzzles to free the cunning monster. Your duty is to rescue this monster from there. Assume that your golden crown was hidden by your friend.
Now you are going to find the golden greeting card by using objects, hints and solving puzzles. We hope you will find that golden greeting card. One day, the boy became stuck in a house by mistake. Have you ever thought you might be a chicken? Our educational games are easy to use and classroom friendly, with a focus on the alphabet, numbers, shapes, storybooks, keyboarding, money, patterns, art, matching, holidays and much more! Have you ever thought you might be a chicken locked in a room with 4 colored doors leading to some other very strange rooms with tricky puzzles? Escape Game: 50 Rooms 2 is a game from BusColdApp and is available from the Google Play Store. I personally liked the graphics, nicely made illustrations.


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Pray the Devil Back to Hell tells the remarkable story of Nobel Peace Prize winner Leymah Gbowee and the courageous Liberian women who came together to pray, protest, and bring about a peaceful end to a bloody civil war. This inspiring film from Academy Award-nominated director Gini Reticker and acclaimed producer Abigail E. Disney shows how grassroots activism can alter the history of nations.
When you buy a video, your viewing rights don't expire, except as detailed in the Terms of Use. You can Watch Now or Download the video to a compatible device, except for Pay-Per-View videos. Pay-Per-View videos will become available to watch once the event starts, will be available for replay for 24 hours following the event, and are not available for download.
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Listing Information
I'm an average player looking to join a clan to play a variety of games with.
We're interested. Recruiting skilled Fortnite players - Come and chat on our Discord.
We provide Sponsorships, Tournaments and Management.
Discord: https://discordapp.com/invite/Eh9VgXB
Website: https://www.g4me.gg
Realm of Myths is multi region multi platform multi game community. We have a staff team who has managed groups of 2000 plus and a competative team for both frotnite and CoD 4
If interested message me on discord at RedMystikFox#5515
Ghidorah Gamming
Hey Ghidorah Gaming would be so excited to bring you as part of the family here! We have 40 members at the moment and a rapidly expanding! Let's get you in and have ya meet the family! https://discord.gg/wGkpPTb
KNIGHTSOFTERROR is interested our clan plays bot fortnite and call of duty ._.·🎀𝓕𝓑𝓘𝓛𝓸𝓵𝓲𝓖𝓲𝓻𝓵🎀·._.#2170 is my discord message if your interested in us
cant wait to here back!!!
Reignofterror98 (leader of the clan)
Clan name_Recon
Hi i am the co founder of Team ElipTic and we are a very small clan as of the moment. We play in Na East and we are slowly getting more and more sponsers to grow. We do not plan on having any more than 6 players in the org. As of the moment we only have 3. Please contact me on Instagram @team_eliptic


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text: ARTISAN (UK), the Huntingdon-based developer, is set to acquire a four acre site in Peterborough subject to planning consent. The company says the new site - Cygnet Park on the Hampton Estate - is already attracting interest from potential clients. Ultimately, Artisan expects to develop approximately 60,000 square feet of office space on Cygnet Park. Chief executive Chris Musselle said:


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Beyond Borders
Sadio Berahino could ditch England to represent Burundi
Stoke City striker Sadio Berahino has been granted international clearance to play for Burundi, according to the Burundi FA.
Berahino played regularly with England through the youth ranks, winning the 2010 under-17s European Championship.
He was called up for the senior squad in November 2014, but didn't play, meaning he can still represent the country of his birth.
The Burundi FA announced it has gained clearance to call up the 24-year-old striker after they investigated whether he would be able to play for them.
Burundi had to provide FIFA with Berahino's passport, birth certificate and evidence that he had not played at senior level for England.
Berahino fled Burundi at the age of 10 to escape civil war which had claimed the life of his father and currently he is in line for his first cap for Burundi.
He could make his debut for the African nation when they face Gabon next month in the 2019 African Cup of Nations qualifiers.
Jérémie Manirakiza, general secretary of the Burundi Football Federation, is satisfied with FIFA's decision, saying: "Saido Berahino is an experienced player. Burundi will benefit enormously from its experience."
Berahino has also previously discussed his Burundian descent in 2013, saying: "Burundi is motherland to me.
"I will always be a Burundian regardless of what happens, even if I become a successful Premier League player. I will still have the Burundi culture in me," he added.
Burundi are currently 148th in the FIFA rankings, and have never qualified for an Africa Cup of Nations or a World Cup before.
However, they have a chance to qualify for the 2019 AFCON after winning their first game 3-0 against South Sudan. Gabon and Mali are the other two teams in their group.
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† "As much as" equivalent value savings percentages are based upon Preliminary assortment choices; supplies are minimal and our inventory is usually changing.
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