text:
TV-14 | 43 min | Drama, Thriller
After a series of events, the lives of a security operative and an assassin become inextricably linked.
Stars: Sandra Oh, Jodie Comer, Fiona Shaw, Kim Bodnia
Votes: 44,687
R | 98 min | Drama, Thriller
Inspired by classic film noir, Carson Phillips, an ex-football star turned PI, has a soft spot for a lady in distress.
Directors: Francesco Cinquemani, George Gallo | Stars: Famke Janssen, Brendan Fraser, John Travolta, Morgan Freeman
Votes: 1,431
R | 152 min | Fantasy, Horror, Mystery
A darkness swirls at the center of a world-renowned dance company, one that will engulf the artistic director, an ambitious young dancer, and a grieving psychotherapist. Some will succumb to the nightmare. Others will finally wake up.
Director: Luca Guadagnino | Stars: Chloë Grace Moretz, Tilda Swinton, Doris Hick, Malgorzata Bela
Votes: 37,876 | Gross: $2.47M
R | 132 min | Drama, Romance
In 1980s Italy, a romance blossoms between a seventeen-year-old student and the older man hired as his father's research assistant.
Director: Luca Guadagnino | Stars: Armie Hammer, Timothée Chalamet, Michael Stuhlbarg, Amira Casar
Votes: 154,506 | Gross: $18.10M
R | 89 min | Crime, Thriller
A Copenhagen police officer seeks justice for his partner's murder by a mysterious man.
Director: Brian De Palma | Stars: Carice van Houten, Nikolaj Coster-Waldau, Guy Pearce, Søren Malling
Votes: 930
TV-MA | 55 min | Crime, Drama, Thriller
Ciro disregards tradition in his attempt to become the next boss of his crime syndicate. The internal power struggle puts him and his entire family's life at risk.
Stars: Marco D'Amore, Salvatore Esposito, Fortunato Cerlino, Cristina Donadio
Votes: 22,555
60 min | Drama, Mystery, Thriller
In 1327, an enlightened friar and his young apprentice investigate a series of mysterious deaths at an abbey risking the wrath of a powerful Inquisitor. Television adaptation of Umberto Eco's novel 'The Name of the Rose'.
Stars: John Turturro, Rupert Everett, Damian Hardung, Fabrizio Bentivoglio
Votes: 1,048
R | 189 min | Drama, History, Romance
German artist Kurt Barnert has escaped East Germany and now lives in West Germany, but is tormented by his childhood under the Nazis and the GDR-regime.
Director: Florian Henckel von Donnersmarck | Stars: Tom Schilling, Sebastian Koch, Paula Beer, Saskia Rosendahl
Votes: 4,994 | Gross: $1.30M
R | 133 min | Crime, Drama, Mystery
Laura, a Spanish woman living in Buenos Aires, returns to her hometown outside Madrid with her two children to attend her sister's wedding. However, the trip is upset by unexpected events that bring secrets into the open.
Director: Asghar Farhadi | Stars: Penélope Cruz, Javier Bardem, Ricardo Darín, Eduard Fernández
Votes: 18,453 | Gross: $2.66M
TV-14 | 60 min | Biography, Drama, History
A political family drama set in Florence in the early fifteenth century. Cosimo de Medici finds himself at the helm of his banking dynasty when his father, Giovanni, dies suddenly.
Stars: Daniel Sharman, Annabel Scholey, Jacopo Olmo Antinori, Sarah Parish
Votes: 11,706
R | 178 min | Western
A bounty hunting scam joins two men in an uneasy alliance against a third in a race to find a fortune in gold buried in a remote cemetery.
Director: Sergio Leone | Stars: Clint Eastwood, Eli Wallach, Lee Van Cleef, Aldo Giuffrè
Votes: 625,017 | Gross: $6.10M
R | 229 min | Crime, Drama
A former Prohibition-era Jewish gangster returns to the Lower East Side of Manhattan over thirty years later, where he once again must confront the ghosts and regrets of his old life.
Director: Sergio Leone | Stars: Robert De Niro, James Woods, Elizabeth McGovern, Treat Williams
Votes: 278,674 | Gross: $5.32M
NC-17 | 115 min | Drama, Romance
A young American studying in Paris in 1968 strikes up a friendship with a French brother and sister. Set against the background of the '68 Paris student riots.
Director: Bernardo Bertolucci | Stars: Michael Pitt, Louis Garrel, Eva Green, Anna Chancellor
Votes: 100,884 | Gross: $2.53M
Not Rated | 117 min | Drama, Horror, War
In World War II Italy, four fascist libertines round up nine adolescent boys and girls and subject them to one hundred and twenty days of physical, mental and sexual torture.
Director: Pier Paolo Pasolini | Stars: Paolo Bonacelli, Laura Betti, Giorgio Cataldi, Umberto Paolo Quintavalle
Votes: 48,262
PG-13 | 165 min | Western
A mysterious stranger with a harmonica joins forces with a notorious desperado to protect a beautiful widow from a ruthless assassin working for the railroad.
Director: Sergio Leone | Stars: Henry Fonda, Charles Bronson, Claudia Cardinale, Jason Robards
Votes: 272,632 | Gross: $5.32M
R | 108 min | Comedy, Drama, Romance
Amidst the war climate, a teenage boy discovering himself becomes love-stricken by Malèna, a sensual woman living in a small, narrow-minded Italian town.
Director: Giuseppe Tornatore | Stars: Monica Bellucci, Giuseppe Sulfaro, Luciano Federico, Matilde Piana
Votes: 80,169 | Gross: $3.43M
Unrated | 156 min | Drama, History
Details the graphic and shocking, yet undeniably tragic story of Rome's most infamous Caesar, Gaius Germanicus Caligula.
Director: Tinto Brass | Stars: Malcolm McDowell, Peter O'Toole, Helen Mirren, Teresa Ann Savoy
Votes: 31,303 | Gross: $23.44M
Not Rated | 93 min | Comedy, Drama
A happily married 24-year-old woman who experiences an inexplicable, rather restless craving to finally live her life intensely, retells her extra-marital escapades to her husband intending to spice up their marriage.
Director: Tinto Brass | Stars: Claudia Koll, Paolo Lanza, Franco Branciaroli, Isabella Deiana
Votes: 3,219
PG-13 | 116 min | Comedy, Drama, Romance
When an open-minded Jewish librarian and his son become victims of the Holocaust, he uses a perfect mixture of will, humor, and imagination to protect his son from the dangers around their camp.
Director: Roberto Benigni | Stars: Roberto Benigni, Nicoletta Braschi, Giorgio Cantarini, Giustino Durano
Votes: 554,669 | Gross: $57.60M
95 min | Crime, Drama, Mystery
Detective Montalbano solves crimes in Sicily.
Stars: Luca Zingaretti, Peppino Mazzotta, Angelo Russo, Cesare Bocci
Votes: 2,393
PG-13 | 114 min | Action, Crime, Sport
A young FBI agent infiltrates an extraordinary team of extreme sports athletes he suspects of masterminding a string of unprecedented, sophisticated corporate heists.
Director: Ericson Core | Stars: Edgar Ramírez, Luke Bracey, Ray Winstone, Teresa Palmer
Votes: 53,854 | Gross: $28.78M
R | 167 min | Crime, Drama
In 1862, Amsterdam Vallon returns to the Five Points area of New York City seeking revenge against Bill the Butcher, his father's killer.
Director: Martin Scorsese | Stars: Leonardo DiCaprio, Cameron Diaz, Daniel Day-Lewis, Jim Broadbent
Votes: 371,896 | Gross: $77.81M
PG-13 | 111 min | Action, Crime, Thriller
After being betrayed and left for dead in Italy, Charlie Croker and his team plan an elaborate gold heist against their former ally.
Director: F. Gary Gray | Stars: Donald Sutherland, Mark Wahlberg, Edward Norton, Charlize Theron
Votes: 322,657 | Gross: $106.13M
R | 131 min | Crime, Drama, Thriller
Living in exile, Dr. Hannibal Lecter (Sir Anthony Hopkins) tries to reconnect with now disgraced F.B.I. Agent Clarice Starling (Julianne Moore), and finds himself a target for revenge from a powerful victim (Gary Oldman).
Director: Ridley Scott | Stars: Anthony Hopkins, Julianne Moore, Gary Oldman, Ray Liotta
Votes: 237,191 | Gross: $165.09M
TV-MA | 111 min | Crime, Drama
A rebellious teen grows up to become one of Milan's most ambitious criminals during the golden years of the 'ndrangheta Mafia in the 1980s.
Director: Renato De Maria | Stars: Riccardo Scamarcio, Sara Serraiocco, Alessio Praticò, Alessandro Tedeschi
Votes: 2,702
PG-13 | 126 min | Action, Adventure, Thriller
IMF Agent Ethan Hunt comes into conflict with a dangerous and sadistic arms dealer who threatens his life and his fiancee in response.
Director: J.J. Abrams | Stars: Tom Cruise, Michelle Monaghan, Ving Rhames, Philip Seymour Hoffman
Votes: 306,624 | Gross: $134.03M
PG-13 | 115 min | Action, Adventure, Sci-Fi
In 2018, a mysterious new weapon in the war against the machines, half-human and half-machine, comes to John Connor on the eve of a resistance attack on Skynet. But whose side is he on, and can he be trusted?
Director: McG | Stars: Christian Bale, Sam Worthington, Anton Yelchin, Moon Bloodgood
Votes: 321,122 | Gross: $125.32M
R | 161 min | Adventure, Drama, History
In the 17th century, two Portuguese Jesuit priests travel to Japan in an attempt to locate their mentor, who is rumored to have committed apostasy, and to propagate Catholicism.
Director: Martin Scorsese | Stars: Andrew Garfield, Adam Driver, Liam Neeson, Tadanobu Asano
Votes: 85,353 | Gross: $7.08M
R | 175 min | Action, Adventure, Biography
Alexander (Colin Farrell), the King of Macedonia and one of the greatest army leaders in the history of warfare, conquers much of the known world.
Director: Oliver Stone | Stars: Colin Farrell, Anthony Hopkins, Rosario Dawson, Angelina Jolie
Votes: 154,647 | Gross: $34.30M
PG-13 | 138 min | Mystery, Thriller
Harvard symbologist Robert Langdon works with a nuclear physicist to solve a murder and prevent a terrorist act against the Vatican during one of the significant events within the church.
Director: Ron Howard | Stars: Tom Hanks, Ewan McGregor, Ayelet Zurer, Stellan Skarsgård
Votes: 253,060 | Gross: $133.38M
R | 134 min | Drama, Fantasy, Horror
From the bitter quest of the Queen of Longtrellis, to two mysterious sisters who provoke the passion of a king, to the King of Highhills obsessed with a giant Flea, these tales are inspired by the fairytales by Giambattista Basile.
Director: Matteo Garrone | Stars: Salma Hayek, Vincent Cassel, Toby Jones, John C. Reilly
Votes: 24,107 | Gross: $0.08M
PG-13 | 103 min | Action, Adventure, Thriller
Revolves around Frank, an American tourist visiting Italy to mend a broken heart. Elise is an extraordinary woman who deliberately crosses his path.
Director: Florian Henckel von Donnersmarck | Stars: Johnny Depp, Angelina Jolie, Paul Bettany, Timothy Dalton
Votes: 206,868 | Gross: $67.63M
R | 132 min | Biography, Crime, Drama
The story of the kidnapping of 16-year-old John Paul Getty III and the desperate attempt by his devoted mother to convince his billionaire grandfather Jean Paul Getty to pay the ransom.
Director: Ridley Scott | Stars: Michelle Williams, Christopher Plummer, Mark Wahlberg, Romain Duris
Votes: 60,448 | Gross: $25.11M
R | 156 min | Biography, Drama, History
Traudl Junge, the final secretary for Adolf Hitler, tells of the Nazi dictator's final days in his Berlin bunker at the end of WWII.
Director: Oliver Hirschbiegel | Stars: Bruno Ganz, Alexandra Maria Lara, Ulrich Matthes, Juliane Köhler
Votes: 306,455 | Gross: $5.51M
Not Rated | 108 min | Drama, Horror
A grieving couple retreat to their cabin in the woods, hoping to repair their broken hearts and troubled marriage, but nature takes its course and things go from bad to worse.
Director: Lars von Trier | Stars: Willem Dafoe, Charlotte Gainsbourg, Storm Acheche Sahlstrøm
Votes: 106,757 | Gross: $0.40M
PG-13 | 104 min | Horror, Mystery, Thriller
A woman who lives in her darkened old family house with her two photosensitive children becomes convinced that the home is haunted.
Director: Alejandro Amenábar | Stars: Nicole Kidman, Christopher Eccleston, Fionnula Flanagan, Alakina Mann
Votes: 313,830 | Gross: $96.52M
R | 92 min | Horror
An American newcomer to a prestigious German ballet academy comes to realize that the school is a front for something sinister amid a series of grisly murders.
Director: Dario Argento | Stars: Jessica Harper, Stefania Casini, Flavio Bucci, Miguel Bosé
Votes: 68,366
R | 94 min | Horror
Three American college students studying abroad are lured to a Slovakian hostel, and discover the grim reality behind it.
Director: Eli Roth | Stars: Lauren German, Heather Matarazzo, Bijou Phillips, Roger Bart
Votes: 80,492 | Gross: $17.61M
Unrated | 95 min | Adventure, Horror
During a rescue mission into the Amazon rainforest, a professor stumbles across lost film shot by a missing documentary crew.
Director: Ruggero Deodato | Stars: Robert Kerman, Francesca Ciardi, Perry Pirkanen, Luca Barbareschi
Votes: 46,298
R | 113 min | Action, Adventure, Thriller
A botched mid-air heist results in suitcases full of cash being searched for by various groups throughout the Rocky Mountains.
Director: Renny Harlin | Stars: Sylvester Stallone, John Lithgow, Michael Rooker, Janine Turner
Votes: 111,492 | Gross: $84.05M
PG-13 | 118 min | Crime, Drama, Thriller
Tom Ripley is a talented mimic, moocher, forger and all-around criminal improviser; but there's more to Tom Ripley than even he can guess.
Director: René Clément | Stars: Alain Delon, Maurice Ronet, Marie Laforêt, Erno Crisa
Votes: 11,926 | Gross: $0.62M
PG | 98 min | Adventure, Comedy, Fantasy
Barbarella, an astronaut from the 41st century, sets out to find and stop the evil scientist Durand Durand, whose Positronic Ray threatens to bring evil back into the galaxy.
Director: Roger Vadim | Stars: Jane Fonda, John Phillip Law, Anita Pallenberg, Milo O'Shea
Votes: 29,265
R | 130 min | Crime, Drama, Mystery
An intellectually nonconformist friar investigates a series of mysterious deaths in an isolated abbey.
Director: Jean-Jacques Annaud | Stars: Sean Connery, Christian Slater, Helmut Qualtinger, Elya Baskin
Votes: 94,454 | Gross: $7.15M
Not Rated | 161 min | Drama, Fantasy
A French drug dealer living in Tokyo is betrayed by his best friend and killed in a drug deal. His soul, observing the repercussions of his death, seeks resurrection.
Director: Gaspar Noé | Stars: Nathaniel Brown, Paz de la Huerta, Cyril Roy, Olly Alexander
Votes: 61,317 | Gross: $0.34M
R | 129 min | Drama, Fantasy, History
A series of disjointed mythical tales set in first century Rome.
Director: Federico Fellini | Stars: Martin Potter, Hiram Keller, Max Born, Salvo Randone
Votes: 13,553
R | 178 min | Crime, Drama
A woman on the run from the mob is reluctantly accepted in a small Colorado town. In exchange, she agrees to work for them. As a search visits the town, she finds out that their support has a price. Yet her dangerous secret is never far away.
Director: Lars von Trier | Stars: Nicole Kidman, Paul Bettany, Lauren Bacall, Harriet Andersson
Votes: 126,545 | Gross: $1.53M
60 min | Drama | Post-production
Upcoming follow-up series to Paolo Sorrentino's "The Young Pope."
Stars: Jude Law, Stefano Accorsi, Javier Cámara, Cécile de France
R | 87 min | Action, Drama, Sport
Veteran race car driver Sam Munroe and his son, a fellow driver from a small town overcome family and professional conflicts, balancing competition, ego, resentment and a racing nemesis to come out stronger on the other side.
Director: Karzan Kader | Stars: John Travolta, Rosabell Laurenti Sellers, Michael Madsen, Barry Corbin
Votes: 753
R | 111 min | Crime, Drama, Horror
Two psychopathic young men take a family hostage in their cabin.
Director: Michael Haneke | Stars: Naomi Watts, Tim Roth, Michael Pitt, Brady Corbet
Votes: 83,546 | Gross: $1.29M
TV-MA | 50 min | Drama, Family, Romance
A woman recounts the lifelong friendship and conflicts with a girl she met at primary school in Naples during the early 1950s.
Stars: Valentina Acca, Antonio Buonanno, Gennaro Canonico, Pina Di Gennaro
Votes: 4,425


text:
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Check Out the new Remax App
Ask About This Home
Cute home in a great Eastside location, close to Lake Superior, bike paths, parks, skate park, and so much more. Move-in ready this home has an open kitchen and living area with TONS of natural light and gas fireplace as well as a composite deck that's perfect for enjoying those hot summer nights. Deck is complete with hot tub and plenty of room for entertaining and opens up to fenced backyard. Basement is finished with a large family room with gas fireplace, laundry room, storage area, and full bath complete with sauna for those cold winter nights. Upstairs is a HUGE master bedroom with sitting area and built-ins, gas fireplace, walk-in closet and additional storage.
*This option is for a conforming mortgage. For amounts exceeding $484,350, you may need a jumbo mortgage.

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Cobalt Technologies, a leading developer of next generation bio-based chemicals, and specialty chemical company Rhodia, member of the Solvay Group, are to begin joint development and operation of a biobutanol demonstration facility in Brazil. The pilot plant moves the companies a step closer to the ...Availability: OnlineRecord type: News Item
Qatar Airways, Qatar Science and Technology Park and Qatar Petroleum are to jointly carry out engineering and economic analysis for the development of sustainable aviation biofuel, with support from Airbus. Seven months ago, together with US-based Verno Systems, the companies embarked on a feasibili...Availability: OnlineRecord type: News Item
Almost all of the technologies for the production of second generation biofuels are in the final stage of commercialisation and their launch is expected within the next two years. These are biofuels that are derived from non-food biomass such as agricultural and forest waste, or energy crops like mi...Availability: OnlineRecord type: News Item
Although improved technology and lower carbon fuels can make a substantial contribution to meeting carbon dioxide (CO2) reduction targets, a significant change in consumer behaviour will be required for the UK to make the transition to a low carbon economy and meet the UK government's 2050 CO2 reduc...Availability: OnlineRecord type: News Item
Three waste-to-energy plants employing different technologies to generate power and/or heat are at various stages of development in Wales and Derbyshire. First, global waste-to-energy company Covanta Energy is to build a £400mn energy-from-waste plant near Merthyr Tydfil in Wales, following detailed...Availability: OnlineRecord type: News Item
A recently published report from the Technology and Policy Assessment function of the UK Energy Research Centre (UKERC) suggests that up to one fifth of global energy could be provided by biomass without damaging food production. The report reviews more than 90 global studies. A debate has been ragi...Availability: OnlineRecord type: News Item
This book contains a comprehensive review of current industrial cleaning techniques including those using low, medium and high pressure water, solvents, chemical compounds and foam, wet and soluble abrasives and the latest computerised in-line automatic techniques. Emphasis is placed on the practica...Availability: In the libraryRecord type: Book
The overall objective of the GREENFOODS project is to lead the European food and beverage industry to high energy efficiency and reduction of fossil carbon emissions in order to ensure and foster the world wide competitiveness, improve the security of energy supply and guarantee the sustainable prod...Availability: OnlineRecord type: Online Resource
In US, the Boeing-backed second-generation algae biofuels are to be tested by Air New Zealand, Continental Airlines and Virgin Atlantic Airways. Algae's attractions include non freezing at high altitudes (cf. alcohols) and are a non-food, low-water energy source.Availability: OnlineRecord type: Abstract
Student paper: CHRP technology for the food industry. Nyoman Sugiartha. Energy World May 2008, P. 15
Nyoman Sugiartha, of the London School of Engineering and Design, Brunel University, was this year's winner of the Energy Institute's London and Home Counties Branch student paper competition. Here follows a summary of the winning paper.Availability: OnlineRecord type: Abstract


text:
N26 Press Archive
N26 Press Archive
N26 crosses the mark of 3.5 million customers
The Mobile Bank see tremendous demand for its product across Europe
CASH26, N26's cash withdrawal and deposit service, now available in Italy
CASH26 lets N26 customers deposit and withdraw money while they shop, providing a unique and practical solution when it comes to managing cash.
Presse Statement: Current media coverage on BaFin audit
When Maximilian and I founded N26, we had a clear vision: to build the bank the world loves to use. It became our mission to turn a complicated banking experience into a simple and enjoyable one.
Andrea Isola is General Manager N26 Italy
N26 appoints Andrea Isola as General Manager Italy. His goal: drive further growth in the Italian market.
We've reached over 300.000 customers in Italy
Another important milestone for N26 in 2019. Find out more about our 300.000 Italian customers.
N26 announces $300m funding round
N26 has announced a $300 million Series D funding round led by New York-based venture capital and private equity firm Insight Venture Partners, valuing N26 at $2.7bn..
Google Pay per i clienti N26 in Italia
N26 rende disponibile ai suoi clienti italiani la possibilità di aggiungere la propria carta di debito a Google Pay, l'opzione di pagamento mobile veloce, semplice e sicura resa disponibile da Google.
N26 Launches N26 Business Black to Simplify Banking for Freelancers and Self-Employed
N26 today introduces N26 Business Black, a premium business account for freelancers and self-employed individuals who want a bank that's designed to travel wherever their business trips take them.
N26 introduces Spaces, a new feature that gives users maximum flexibility to organize their money
N26 today announced the launch of Spaces, a new feature that provides a fast and easy way to set aside money based on users' personal preferences and needs.
N26 now reached more than €1 billion in monthly transaction volume and more than 1 million customers
N26, one of the fastest growing mobile banks in Europe, now serves more than one million customers across the continent. It has doubled its customer base over the last nine months.


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Most importantly, it is cheaper than most other types of water purifiers available in the market today. You can get pure water every day at an affordable rate. If you do not have a good home water purifier, it might be a good idea to get one as soon as possible.
Location: Naupada Thane West, Thane, Maharashtra 400602
441 Total post views
OGG's Handpick Unique Aesthetics Online Mag.
Oh Good Goods is an Independent Designer Brand Shop.
Here curated by Designer Niki Yu @cubey_studio .
Available Collab/Work/Partnership.
Oh Good Goods: @ohgoodgoods
Another Design Curating: @p-e-a-c
【Goods Collection】:
#fashion #style #outfits #streetwear #apparel #art #decor #shop #ohgoodgoods #streetfashion #be
WeTransfer is the simplest way to send your files around the world - allowing you to stay in your creative flow.
Defringe is a curated online gallery that filters creative content on the web. With the idea of a gallery in our minds, we want the site to act as platform for innovative and creative ideas.
Digital Decade is looking for Ello-based artists to submit artworks and imagine how we may reinvent and edit our identities in the future - Post Identity - Digital Decade 6
1814 is a limited edition, bi-annual publication that focuses on photography, art, design and culture.
Editor in Chief at Sgustok Magazine, Art Director at Sgustok Studio, Record Producer at Sgustok Music, Product Designer at Revolut.
Film Director & Producer
Lead @ellofilm
Consultant for artist and designers / Founder of Meraki Agency (http://merakiagency.nl)
Contact: email@example.com
The Floating Crew promotes the work of young international contemporary artists.
Our goal is to kindle collaborations between artists from different cultural backgrounds and artistic schools to encourage varied and
multi-faceted approaches to art forms and themes.
Multifaceted arts community in Denver, CO. Opened in 2013, Helikon has earned a reputation as a high level exhibition space for contemporary Illustrative Arts, ranging from portraiture, illustration, and figure work to imaginative and narrative art from around the world.
publisher | sfgirlbybay. hardwired to share. extroverted introvert. cultural oddity.
the Kiosk of Democracy is an on-line platform & collection featuring international artists: art, documentation, photo, text, drawings, film, .. with intense content and with an ethical as well as aesthetical position.
the kiosk of democracy is understood as cosmo cultural and cosmopolitan - a cosmo culture collective collection.
the kiosk of democracy organized in 2012 and 2013 exhibitions in Hannover / Germany.
Established was the kiosk of democracy in january 2012 by Sascha Windolph in Hannover / Germany.
More informationen: http://kioskderdemokratie.blogspot.de/2013/03/kiosk-of-democracy-kiosk-der-demokratie.html
contat: Sascha Windolph - chanarie69@gma
Fashion curator, master eye roller, reserved extrovert.
10% more banana.
Italian woman with a nordic soul.
Knit designer and stationary maker from Milan, Italy.
Snail mail, plants and books please.
Design | Art Direction | Objects
Artist- Chris Swasta of Rolling Hills Pottery
📍 Little Rock, Arkansas
Everything Handmade Ceramics
#maker #ellosculpture #ellohandcrafted #ellohandmade #artofello #ellooftheday #ellomaker #artistsofello #artistsonello #ellomaker #ellopottery #elloclay #elloceramics #elloceramicist #ellofeature #earth #stone #stoneware #glaze #handmadepottery #pottersofello #follow #painting #elloartist #artistlife #ellofeature #elloart #ello #ceramics #feature
@ellomakers @ellotextures @ellominimal @ellonew @ellousa @ellodesign @ello @elloart @ello3d @elloabstract @elloblog @ellopatterns
Artist Haris Panousopoulos saved for himself the finest materials for making art.
Berlin-based architect / weaver.
Slow fashion handwoven with natural fibers.
Instagram www.instagram.com/aniagrzeszek
Etsy www.etsy.com/shop/aniagrzeszek
I make fashion and textile art with a household bleach
Artist, Quilter, Designer, Printmaker
a sculptor creating pottery for your everyday // earth + pigment


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Stock Market Prediction 8 February 2019
Stock Market Prediction 18th January 2019
Stock Market Prediction 14th January 2019
Stock Market Prediction 21th November 2018
Stock Market Prediction 21th May 2018
Published on 29/04/2019 12:04:06 PM | Source: Ganeshaspeaks.com
Now Get InvestmentGuruIndia.com news on WhatsApp. Click Here To Know More
* resently two planets are retrograde, so there will be volatility in the market. Due to this, you may get into problems as per the stars,
* However, Neechbhang Rajyog of Mercury and Venus and exalted Sun will give support to the market.
* From 9:15 to 9:45 Nifty will be down trend from surface.
* From 9:45 to 10:30 there will be technical bounce in Nifty.
* From 10:30 to 13:45, we will see a very strange pattern. Here you will have to frequently reshuffle your trade. In this time slot, during the early part and the later part Nifty will be down. There will be some jump in between.
* From 13:45 to 15:00 Nifty will be up.
* In the last 30 minutes, there will be buying pressure.
Views express by all participants are for information & acadamic purpose only. Kindly read disclaimer before refering below views. Click Here For Disclaimer
Hyundai Venue: What`s Good & What Could`ve Been Better
Boeing says sorry for MAX 737 crashes, promises to learn lessons
Total ban on liquor, non-vegetarian food around Varanasi temples
Hyundai Venue CarDekho Round-up: Buyer`s Guide
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I have been at a plateau for 4-6 months now. I've been Keto for over 2 years and have decrease weight and bodyfat, but I can't seem to shake the remainder of the weight. I test my ketone levels several times per week and am usually at .4 to .9 mml. I'm probably eating too many calories from fat (cheese and fatty meats) but I can't seem to get satiated if I eat less. I'd like to get to around 15% bodyfat. I started at 32% as measured by a dexa scan a couple of years ago and dropped to 26% a few months ago.
"It's easy to become impatient and frustrated when you're trying to lose weight and haven't seen the results yet. But be realistic – you won't see the affect overnight. Your brain's wiring plays a huge part in resisting changes in lifestyle, and it takes time to establish new habits – up to 12 weeks. Stick with it for at least eight weeks and you should notice a change."
The healthiest way to lose weight quickly combines a healthy and nutritious diet, proper hydration, and a moderate and varied exercise routine. If you use a fad diet or other rapid weight loss technique to drop a few pounds very quickly, switch after a few days or weeks to a long-term weight loss plan that promotes healthier weight loss to protect your health and promote sustainable weight loss.
A 2012 study also showed that people on a low-carb diet burned 300 more calories a day – while resting! According to one of the Harvard professors behind the study this advantage "would equal the number of calories typically burned in an hour of moderate-intensity physical activity". Imagine that: an entire bonus hour of exercise every day, without actually exercising. A later, even larger and more carefully conducted study confirmed the effect, with different groups of people on low-carb diets burning an average of between 200 and almost 500 extra calories per day.
Eating too little can be extremely dangerous for your body. According to Medical News Today, having a Body Mass Index (BMI) of under 18.5 can lead to malnutrition, osteoporosis, developmental problems, a weakened immune system, anemia, and chronic fatigue. Healthline reports that the average woman needs about 2000 calories per day to maintain her weight and about 1500 calories to lose one pound of weight per week, though you should consult with your healthcare provider to see what's best for you.
If all you have is two weeks to lose weight, you're going to have to be very strict with this rule. Luckily you can still drink any water-based drink such as coffee and tea, so it's not all bad. I usually have a huge glass of ice cold water plus one or two large cups of coffee in the morning to kick start my day. I often use cinnamon to flavour my coffee – it's a great tip for those of you that really enjoyed adding milk.
I'm 14 and I recently lost 7 pounds. I was first 133 and now I'm 126 lb.. I go to gym regularly after school Mon. – Thurs. and have an active after school activity on Fridays. I usually take the weekend off, and I'm planning Sunday is my cheat day. I want to loose 20 more pounds, or first, 6-10 pounds for the first two weeks. I don't have any special food here in my country or have someone who knows how to make it. I'm fine with exercise, but I also have these cravings A LOT. I want to know what I should eat because I'm chubby and people make fun of me and I want to show them what I can do. I also want to be healthy. 🙂
Wow! I truly believe that I read this post today for a reason! I have been taking anxiety medication for the past 5 years and have gained almost 70 lbs in these 5 years. I've literally gone to doctor after doctor about my weight gain and no one could determine why I've gained so much weight. I've literally gone back and forth about quitting cold turkey and was so fearful about the withdrawl side effects. I feel like my weight gain is causing more anxiety than why I'm on the medicine to begin with. I can't thank you enough for sharing your story and motivating me. Thank you!!
Dear Stefani, I am 65 years old and I would like to ask, what is your advice for post-menopausal women in regard to carbo-cycling? For the past 8 months I have practiced IF (fasting nearly daily, between 16 and 24 hours) combined with carbo-cycling (extremely low carbs for 3 or 4 days in a row followed by 1 day of carb reloading) to dramatically reduce my calorie intake while eating nutritious foods only. As a result, I have lost 70 pounds. Now that I am at a good weight and feeling well (I exercise daily), I would like to think I can use IF and carbo-cycling when necessary to avoid regaining weight. But I am concerned by the possibility that I may actually be undermining my body's insulin sensitivity.
Or skip your favorite early-morning show—whatever it takes to grab a few more minutes of sleep each day. When researchers at the University of Chicago studied men who were sleep-deprived, they found that after just a few days, their bodies had a much harder time processing glucose in the blood—a problem common in overweight diabetics. When the individuals returned to a more normal seven to eight hours of sleep a night, however, their metabolisms returned to normal.
About: Ruzele's had weight issues her whole life, but it wasn't until after her mother passed away in 2011 that the pounds really started to pile on. She ballooned up to 335 pounds and had a rock-bottom moment that led her to where she is today: 50 pounds lighter and on a mission to lose 130 more. Ruzele's blog posts are short and sweet, but always delightful and full of musings, emotions, progress reports and the occasional vlog (which work oh-so-perfectly). Ruzele's one to follow if you're looking for someone who's accountable and gets to the point, but still leaves you rooting her on day-in and day-out.
About: Annamarie's weight loss story is nothing to sneeze at. She lost a staggering 180 pounds, and she did it all naturally, by eating right and exercising. These days, Annamarie's in two modes: maintain her healthy weight and lifestyle...and share her personal tips and victories along the way. She's the kind of blog you'll want to follow if you want to tap into the day-by-day challenges (and empowerment) of a young woman who made it happen...and wants you to as well.
For some, as little as one or two drinks can impact weight loss. Beyond just its contribution of calories, alcohol interferes with the digestion and utilization of other nutrients, including fat. If you're hitting a weight plateau and drink alcohol, evaluate your alcohol intake. Consider the type and the volume of alcohol you're consuming. The carbohydrates and calories can add up quickly, and not to mention, we sometimes tend to reach for food when drinking – whether hungry or not!
#3 – Eat breakfast! As Mom always said it's the most important meal of the day and it is. Your body needs fuel to speed up your metabolism and to get you going. I like to eat yogurt with fruit and granola, or oatmeal with Greek Yogurt, and sometimes eggs and pancakes. I like to eat about 300 calories for breakfast. Having full hearty meals, with protein, a pinch of fat and carbs will keep me filled for 3 or 4 hours.
About: Blogs full of heart-felt writing and deep emotions are great, but it never hurts to toss in one with a laugh-out-loud humor to it as well. Enter Running off the Reese's blogger Cely. If her blog mantra "Because no one should have to choose between their pants and chocolate" doesn't pull you in, her creative use of gifs and humorous style of writing will. Cely was diagnosed with rheumatoid arthritis at the age of 18 — a diagnosis she certainly didn't let hold her back. She runs races (usually half-marathons or less), shares her experiences, has a long list of book reviews...and talks a bit about life in general in between. And it's awesome.
Monica Olivas is a holistic health coach, certified running coach, and writer for the blog Run Eat Repeat. She has been a passionate runner for almost 10 years and has completed over 50 half marathons and 30 full marathons. Running has helped her lose 20 pounds and uses her blog as a way to share healthy recipes, running tips, and motivation to help you do the same!
I'm 5′ 2″ or so, and about 102 pounds. I'm 19. Not that long ago, I was only 94 or so, and I eat very healthy (lean protein, fruits, vegetables). My one "no-no" is Starbucks in the morning, but I've always done that, even when I was thin. I want to drop the 8 pounds I gained ASAP. I used to be very tone, and I feel like all that has gone away. What do I need to do. Please help.
A common misconception amongst many female fitness enthusiasts is that steady-state cardio in the "fat burning zone" is ideal for weight loss. Spending two hours on the treadmill may seem like it'll get you bikini-worthy bod—more minutes, more calories, right? However, while steady-state cardio has many benefits, it isn't the most effective way to reduce body fat.[1]
Chronic migraines were what first inspired Amanda Tagge to start exercising. "I was hoping to find some relief from my headaches and working out did help but I realized that if I really wanted to feel better I needed to revamp my health habits overall and lose weight," she says. The more she changed, the better her headaches got and she lost 70 pounds in the process which helped her feel even better. Focusing on all the ways her health was improving kept her going even when the scale wasn't moving.
When I was younger, I'd managed to maintain my weight of 130 pounds by being active. I belonged to the dance team and played intramural sports, so it wasn't difficult for me to motivate myself to exercise. After college, I started a desk job, and with that came long hours, pizza lunches, and plenty of happy hours. I continued to exercise almost every day, but it wasn't enough and my weight climbed to 153 pounds, which was too much for my 5-foot-4-inch frame.
Thank you for sharing your story and your advice! Very good advice! I don't think you look gross above…I think you look happy. Never call yourself gross…its not a healthy thought. YOU are beautiful…your size doesn't define you. I am soo proud of you though…taking such an interest in your health and habits and helping others, AND loosing weight and feeling better all around. What a beautiful thing!
"Feeling stressed can wreak havoc on our bodies. It can cause our body to produce the steroid hormone cortisol, which can make you crave sugary foods that provide instant energy and pleasure. Short-term bursts of cortisol are necessary to help us cope with immediate danger, but our body will also release this hormone if we're feeling stressed or anxious. When our cortisol levels are high for a long amount of time, it can increase the amount of fat you hold in your belly."
I've been keto for 2 months now and lost 14lbs the first two weeks. I haven't lost anything in over a month so I feel a bit frustrated. I've been very disciplined and I just worry that I won't lose anymore. Is this stall normal? I have a little over 40lbs that I'd like to lose and since I'm quite short and suffering from endometriosis, my weight is primarily in my thighs and hips and it seems to take the longest to lose. My disorder also affects my energy levels to due to the hormonal issues. I just really would like to see success eventually because I feel like I'm at my wits end.
Getting healthier and losing weight is among the most popular new year's resolutions for good reason, but sticking with it is easier said than done. If one of your goals in 2019 is weight loss, you're in luck. There are many ways to get motivated to start losing weight and build success that can last a lifetime. Let's make this year the best yet, by sticking to our weight loss resolutions with help from these tips:
Thanks for sharing:-). I find myself in a difficult situation. I had a slip and fall accident back in 2009. My injury was misdiagnosed and the physical therapy made things worse. To the point were I cannot even do aqua therapy. I suffer with chronic pain and was always an energetic, healthy, exercise driven person. I used to weigh in at 102-105 lbs. Now due to inactivity I'm about 137lbs. I am so happy to have found your website. I need help, I am home all the time due to the fact that I cannot sit for long because of a coccyx injury. I am so unhappy with my weight gain. Do you have an encouraging word for me today? I've tried everything I know to do. My dr told me " I'm stuck". But I don't want to give up. Looking forward to your reply.
I'm newer to your blog, and all of your pictures screamed to me "effortlessly thin chick", so imagine my open-mouthed surprise with your post today. At risk of sounding weird, i love your blog and you even more for having been so transparent! Weight is something i've struggled with my whole life, and in the last 8 years, i've also been dealing with an underactive thyroid. Some of the blogs i read have such beautifully thin bloggers pitching things like s'mores, and i think to myself, yeah right. So thanks. Thanks for being so real and relatable! Honesty is the best policy, and you will have helped so many for just laying it all out there truthfully. For many, if not most, it's a struggle. God bless you!
Do you mindlessly sprinkle salt on your food without even tasting it first? Stop it! Seriously. Extra sodium can lead to water weight gain and bloating, which will make your pants fit tighter (and the number on the scale tick up). Be sure to read your labels, too; sodium is typically lurking in in processed foods, even "healthy" ones like frozen dinners and canned soups.
Your weight loss story is soo interesting. I also lost some wieght 4 years ago with UN diet , but as u said it was much harder to maintain this weight then loosing it. Now I need to start again some diet and I found this Keto diet. I think its interesting , but anyway wish me luck.. 🙂 And if someone is interested in KETO diet I found this amazing guide that can be helpful to u too. U can check it out on this link :http://bit.ly/2sekQmx
I acknowledge that you may want to lose weight for any number of reasons. It's not my job to tell you what your motivation for weight loss should be. It is my job to help guide you in that journey to greater health, energy, and love. My goal is to teach women how to love themselves above all else, and then let weight loss follow gently in its path.
Research shows this intensity combo also makes you faster. In a 2013 study published in the Journal of Applied Physiology, researchers found that when cyclists performed six weeks of 80/20-style training, they more than doubled their power and performance gains, such as lactate threshold, compared to when they spent more time in moderate training zones.
About: Laura is a health coach whose passion to help others overcome addiction and gain a satisfying life comes from her own history: For years she battled addiction (to food, cigarettes and many other things) and was taught to believe that as a woman, she was less valuable than a man. Trapped in her own mind and truly miserable, Laura one day realized that the key to overcoming her addictions was to make deliberate choices for every single thing she did — a mindset that taught her just how valuable she is. Now, she's an extraordinarily strong-willed champion of women's health who shares her knowledge and expertise with others. A true inspiration on so many levels.
Want a flatter stomach in two seconds? Stand up straight! Slouching emphasizes belly rolls but straightening your spine elongates your whole body, making you look taller and sleeker. Want to go even flatter for a picture? Use the old modeling trick and arch your back slightly—this will pull your skin tighter across your stomach while moving it farther away from the camera, making it look slightly smaller. Yeah, it's a temporary fix but good posture offers many health benefits beyond looking good.
Ultimately, you need to pick a healthy eating plan you can stick to, Stewart says. The benefit of a low-carb approach is that it simply involves learning better food choices—no calorie-counting is necessary. In general, a low-carb way of eating shifts your intake away from problem foods—those high in carbs and sugar and without much fiber, like bread, bagels and sodas—and toward high-fiber or high-protein choices, like vegetables, beans and healthy meats.
Another frontrunner on the U.S. News and World Report 2016 list (it came in at number two in the weight loss category), the HMR Weight Management program is used in over 200 medical facilities around the U.S. Dieters embark on two phases, the first centered around HMR's products (meals, shakes, snacks) and the second transitioning towards a sustainable plan emphasizing fruits, vegetables, lean proteins, and whole grains.
Sure, there's your one friend who swears by the Taco Cleanse. And that other friend who ate nothing but broccoli soup for a month and dropped 20 pounds, found the love of her life, and got promoted at work. But before you start blending 80 stalks of broccoli into a cup or crunching your way through a crate of tacos, check out which diets are backed by science. Because don't you want to try one that will do the trick for you?
About: Rachel, Valeri and Dori are three lifelong, childhood friends who share a very strong common bond: They all were fat, and they're all now fit. But they're also very unique — each has her own approach to weight loss and fitness, approaches that come together for one heck of a diverse blog. No matter what your level of fitness is or how much weight you need to lose, you'll find a style from one of these ladies that resonates. Inspirational, logical and practical, Rachel, Valeri and Dori truly put together a masterpiece.
Everyone is different. How quickly you burn calories when you are not physically active can be very different from other people based on your specific genes, biology, and past. While scientists know that there are 3,500 calories in one pound, simply eating 500 fewer calories every day for a week (or 3,500 fewer calories in a week) does not always end in losing exactly one pound.
It is important to understand that weight is entirely a function of input and output. The input is the food you eat and the calories contained therein. The output is your energy output. To lose weight the output needs to be greater than the input. It is that simple. Do not believe any of the diet fads. If you are currently not gaining or losing weight then just burning 300 extra calories per week or eating/drinking 300 calories less per week (2 sodas for example or a small burger) WILL make you lose weight - in this case around 5 pounds of fat per year.
Ross Enamait is a boxing coach and trainer. He has a passion for high-performance conditioning, strength, and athletic development. His philosophy is that successful training requires figuring out what works for the individual. On Ross Training, he provides the research and real-world advice his experiences have backed up, but never a "my way or the highway" approach. Visit the blog.


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KOLKATA: With the Mamata Banerjee government yet to initiate action against Trinamool Congress MP Tapas Pal for his rape remarks, a PIL was filed Wednesday seeking penal action.
The public interest litigation (PIL) was filed before the court of Justice Dipankar Dutta of the Calcutta High Court seeking a direction to the police for initiating criminal proceedings and arrest of Krishnanagar MP Pal.
"He has publicly exhorted his partymen to kill CPI-M activists and rape women. But, still this government has not initiated any action. So, we have pleaded before the court to take cognisance and direct the police to initiate criminal proceedings and arrest Pal," counsel for petitioner Subrata Mukherjee said.
Addressing his party workers in his constituency, Pal was caught on subsequent tapes threatening to gun down CPI-M men and let loose his boys to rape their women. He also asked people to slit the rivals' throats to prove themselves.
"If a CPI-M man dares to touch anybody, I will take out my gun and shoot him...I will destroy his entire clan... I will ask my boys to go there. They will rape them and leave," Pal is seen saying in the first video while addressing partymen in Choumaha village.
Proceeding to Gopinathpur, 10 km from Choumaha, Pal is seen in the second video asking women to slit CPI-M throats and exhorts people to kill their rivals and show him.
"Till I am here, don't spare any CPI-M worker. Our men will kill them. I am also asking the women to slit open CPI-M workers' throats. Use your bontis (sharp vegetable-cutting instrument) to slash their throats. Why haven't you done it till now? Don't you know how to use a knife?
"I will shoot them in front of everyone.... Kill two to four people and show me.... Kill them with an axe and prove yourself. Then I will see...." says Pal in the second video which became public Tuesday when he had tendered an apology for his earlier comments.
There has been countrywide outrage over Pal's remarks with all and sundry seeking his arrest and expulsion from parliament.
Editorial: Electoral Win No Licence for Parties to Run Amok
Also Read:
Mamata Trying to Shield Tapas Pal: Siddharth Nath Singh
Pal Buckles, Tenders Apology for Remarks


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AWIN is probably best for experienced affiliates who can hit the ground running without a lot of guidance or feedback from the network. There is a $5 fee charged to apply to become an affiliate, but if you're approved, the $5 will be added to your account. If your application is denied, however, you will lose the $5 fee. AWIN operates globally, but it is most heavily concentrated on British and EU merchants.
Despite its older origins, email marketing is still a viable source of affiliate marketing income. Some affiliates have email lists they can use to promote the seller's products. Others may leverage email newsletters that include hyperlinks to products, earning a commission after the consumer purchases the product. Another method is for the affiliate to cultivate email lists over time. They use their various campaigns to collect emails en masse, then send out emails regarding the products they are promoting.
Unfortunately, the 2Checkout dashboard is a bit limited in scope, making it difficult to get any metrics on conversion rates or even sorting by commission payouts. The workaround is to go to the Avangate store, which does list their best-selling products, and then search for these on the affiliate dashboard. That being said, 2Checkout does offer products from more than 4,000 different vendors, making it the leading affiliate network for software and digital products.
Do you have any information on how to set up and link Landing Page, Thank you page, etc. in Thrive (For an Affiliate funnel) like you do with Clickfunnels? I have read and watched several times (above) but am not able to convert Clickfunnel understanding to Thrive. I have watched several tutorials in Thrive University and haven't yet seen how to do that. Any assistance is greatly appreciated; Thank you!
In 2006, the most active sectors for affiliate marketing were the adult gambling, retail industries and file-sharing services.[21]:149–150 The three sectors expected to experience the greatest growth are the mobile phone, finance, and travel sectors.[21] Soon after these sectors came the entertainment (particularly gaming) and Internet-related services (particularly broadband) sectors. Also several of the affiliate solution providers expect to see increased interest from business-to-business marketers and advertisers in using affiliate marketing as part of their mix.[21]:149–150
The problem with affiliate marketing, like many other home business options, are the so-called gurus and get-rich-quick programs that suggest affiliate marketing can be done fast and with little effort. Odds are you've read claims of affiliate marketing programs that say you can make hundreds of thousands of dollars a month doing almost nothing ("Three clicks to rich!"). Or, they suggest you can set up your affiliate site, and then forget it, except to check your bank deposits.


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NCBI: db=pubmed; Term=metabolomics
Updated: 13 min 27 sec ago
Associations between usual food intake and fecal sterols and bile acids: results from the KORA FF4 study.
Related Articles Associations between usual food intake and fecal sterols and bile acids: results from the KORA FF4 study. Br J Nutr. 2019 Jun 11;:1-26 Authors: Mitry P, Wawro N, Sharma S, Kriebel J, Artati A, Adamski J, Heier M, Meisinger C, Thorand B, Grallert H, Peters A, Linseisen J Abstract Animal sterols, plant sterols and bile acids in stool samples have been suggested as biomarkers of dietary intake. It is still unknown whether they also reflect long-term habitual dietary intake and can be used in aetiological research. In a subgroup of the KORA FF4 study, habitual dietary intake was estimated based on repeated 24HFL and a FFQ. Stool samples were collected according to a SOP and those meeting the quality criteria were extracted and analysed by means of a metabolomics technique. The present study is based on data from 513 men and 495 women with a mean age of 60 and 58 years, respectively, for which fecal animal and plant sterols and bile acids concentrations and dietary intake data were available. In adjusted regression models, the associations between food intake and log-normalised metabolite concentrations were analysed. Bonferroni correction was used to account for multiple testing. In this population-based sample, associations between habitual dietary intake and fecal concentrations of animal sterols were identified, while the impact of usual diet on bile acids was limited. A habitual diet high in 'fruits' and 'nuts and seeds' is associated with lower animal fecal sterols concentrations, whereas a diet high in 'meat and meat products' is positively related to fecal concentrations of animal sterols. A positive association between glycocholate and fruit consumption was found. Further studies are necessary for evaluation of fecal animal sterols as biomarkers of diet. The findings need to be confirmed in other populations with diverse dietary habits. PMID: 31182174 [PubMed - as supplied by publisher]
Taxol-Loaded MSC-Derived Exosomes Provide a Therapeutic Vehicle to Target Metastatic Breast Cancer and Other Carcinoma Cells.
Related Articles Taxol-Loaded MSC-Derived Exosomes Provide a Therapeutic Vehicle to Target Metastatic Breast Cancer and Other Carcinoma Cells. Cancers (Basel). 2019 Jun 09;11(6): Authors: Melzer C, Rehn V, Yang Y, Bähre H, von der Ohe J, Hass R Abstract MSC-derived exosomes display, among others, an efficient biocompatibility and a reduced intrinsic immunogenicity, representing a valuable vehicle for drug delivery in a tumor-therapeutic approach. Following treatment of several human mesenchymal stroma/stem-like cell (MSC) populations with sub-lethal concentrations of taxol for 24 h, exosomes were isolated and applied to different human cancer populations including A549 lung cancer, SK-OV-3 ovarian cancer, and MDA-hyb1 breast cancer cells. While MSC control exosomes revealed little if any growth inhibition on the tumor cells, exposure to taxol-loaded MSC-derived exosomes was associated with 80-90% cytotoxicity. A similar application of taxol-loaded exosomes from HuVEC displayed much fewer effects. Quantification by LC-MS/MS analysis demonstrated a 7.6-fold reduced taxol concentration in MSC exosomes when compared to equivalent cytotoxic in vitro effects achieved with taxol substances, indicating a specific and more efficient tumor-targeting property. Consequently, MSC-derived taxol exosomes were tested in vivo. Highly metastatic MDA-hyb1 breast tumors were induced in NODscid mice, and systemic intravenous application of MSC-derived taxol exosomes revealed a more than 60% reduction of subcutaneous primary tumors. Moreover, the amount of distant organ metastases observed at least in lung, liver, spleen, and kidney was reduced by 50% with MSC taxol exosomes, similar to the effects observed with taxol, although the concentration of taxol in exosomes was about 1000-fold reduced. Together, these findings in different cancer cell populations and in vivo provide promising future perspectives for drug-loaded MSC-derived exosomes in efficiently targeting primary tumors and metastases by reducing side effects. PMID: 31181850 [PubMed]
Snail-Overexpression Induces Epithelial-mesenchymal Transition and Metabolic Reprogramming in Human Pancreatic Ductal Adenocarcinoma and Non-tumorigenic Ductal Cells.
Related Articles Snail-Overexpression Induces Epithelial-mesenchymal Transition and Metabolic Reprogramming in Human Pancreatic Ductal Adenocarcinoma and Non-tumorigenic Ductal Cells. J Clin Med. 2019 Jun 08;8(6): Authors: Liu M, Hancock SE, Sultani G, Wilkins BP, Ding E, Osborne B, Quek LE, Turner N Abstract The zinc finger transcription factor Snail is a known effector of epithelial-to-mesenchymal transition (EMT), a process that underlies the enhanced invasiveness and chemoresistance of common to cancerous cells. Induction of Snail-driven EMT has also been shown to drive a range of pro-survival metabolic adaptations in different cancers. In the present study, we sought to determine the specific role that Snail has in driving EMT and adaptive metabolic programming in pancreatic ductal adenocarcinoma (PDAC) by overexpressing Snail in a PDAC cell line, Panc1, and in immortalized, non-tumorigenic human pancreatic ductal epithelial (HPDE) cells. Snail overexpression was able to induce EMT in both pancreatic cell lines through suppression of epithelial markers and upregulation of mesenchymal markers alongside changes in cell morphology and enhanced migratory capacity. Snail-overexpressed pancreatic cells additionally displayed increased glucose uptake and lactate production with concomitant reduction in oxidative metabolism measurements. Snail overexpression reduced maximal respiration in both Panc1 and HPDE cells, with further reductions seen in ATP production, spare respiratory capacity and non-mitochondrial respiration in Snail overexpressing Panc1 cells. Accordingly, lower expression of mitochondrial electron transport chain proteins was observed with Snail overexpression, particularly within Panc1 cells. Modelling of 13C metabolite flux within both cell lines revealed decreased carbon flux from glucose in the TCA cycle in snai1-overexpressing Panc1 cells only. This work further highlights the role that Snail plays in EMT and demonstrates its specific effects on metabolic reprogramming of glucose metabolism in PDAC. PMID: 31181802 [PubMed]
Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics.
Related Articles Characterization of Bulk Phosphatidylcholine Compositions in Human Plasma Using Side-Chain Resolving Lipidomics. Metabolites. 2019 Jun 08;9(6): Authors: Quell JD, Römisch-Margl W, Haid M, Krumsiek J, Skurk T, Halama A, Stephan N, Adamski J, Hauner H, Mook-Kanamori D, Mohney RP, Daniel H, Suhre K, Kastenmüller G Abstract Kit-based assays, such as AbsoluteIDQTM p150, are widely used in large cohort studies and provide a standardized method to quantify blood concentrations of phosphatidylcholines (PCs). Many disease-relevant associations of PCs were reported using this method. However, their interpretation is hampered by lack of functionally-relevant information on the detailed fatty acid side-chain compositions as only the total number of carbon atoms and double bonds is identified by the kit. To enable more substantiated interpretations, we characterized these PC sums using the side-chain resolving LipidyzerTM platform, analyzing 223 samples in parallel to the AbsoluteIDQTM. Combining these datasets, we estimated the quantitative composition of PC sums and subsequently tested their replication in an independent cohort. We identified major constituents of 28 PC sums, revealing also various unexpected compositions. As an example, PC 16:0_22:5 accounted for more than 50% of the PC sum with in total 38 carbon atoms and 5 double bonds (PC aa 38:5). For 13 PC sums, we found relatively high abundances of odd-chain fatty acids. In conclusion, our study provides insights in PC compositions in human plasma, facilitating interpretation of existing epidemiological data sets and potentially enabling imputation of PC compositions for future meta-analyses of lipidomics data. PMID: 31181753 [PubMed]
metabolomics; +18 new citations
18 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: metabolomics These pubmed results were generated on 2019/06/11PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
metabolomics; +18 new citations
18 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: metabolomics These pubmed results were generated on 2019/06/11PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Metabolomics of the alimurgic plants Taraxacum officinale, Papaver rhoeas and Urtica dioica by combined NMR and GC-MS analysis.
Metabolomics of the alimurgic plants Taraxacum officinale, Papaver rhoeas and Urtica dioica by combined NMR and GC-MS analysis. Phytochem Anal. 2019 Jun 09;: Authors: Grauso L, Emrick S, Bonanomi G, Lanzotti V Abstract INTRODUCTION: The phytoalimurgic plants, common dandelion (Taraxacum officinale), corn poppy (Papaver rhoeas) and stinging nettle (Urtica dioica) are a source of nutraceuticals. OBJECTIVES: To apply a combined metabolomic fingerprinting approach by nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS) to common dandelion, corn poppy and stinging nettles to obtain simultaneous identification and quantitation of the major classes of organic compounds. METHODOLOGY: The whole plants collected in the Cilento National Park were dried and then extracted to obtain non-polar and polar organic extracts. GC-MS was used for non-polar extracts while 1 H-NMR spectroscopy was used for polar extracts. In both cases, simultaneous identification and quantification of the bioactive metabolites was obtained. RESULTS: Non-polar organic extracts of all plants were mainly composed of palmitic, stearic and oleic acids. The two pentacyclic triterpenols α- and β-amyrin were detected in nettle extract. The analysis of polar organic extracts allowed to detect and quantify organic acids and sugars as main metabolites along with amino acids, caffeoyl derivatives, flavonoids, and nucleotides. In particular, corn poppy leaves contained a huge amount of glyceric acid (55.7% of the total extract). Stinging nettles, instead, exhibited a large amount of choline (19.5%). CONCLUSION: Metabolomic approach coupling GC-MS with NMR spectroscopy allowed to provide a detailed metabolite profile of three alimurgic plants, common dandelion, corn poppy and stinging nettle, from both a qualitative and quantitative point of view. PMID: 31177603 [PubMed - as supplied by publisher]
UPLC-QTOF/MS-based metabolomics reveals the mechanism of chronic unpredictable mild stress-induced hypertension in rats.
UPLC-QTOF/MS-based metabolomics reveals the mechanism of chronic unpredictable mild stress-induced hypertension in rats. Biomed Chromatogr. 2019 Jun 08;:e4619 Authors: Wu Q, Xia DM, Lan F, Wang YK, Tan X, Sun JC, Wang WZ, Wang R, Peng XD, Liu M Abstract Hypertension is a common chronic disease, and it is the strongest risk factor for cardiovascular diseases. Recently, the number of patients with hypertension-related complications has increased significantly, adding a heavy burden to the public health system. It is known that chronic stress plays an important role in the pathogenesis of cardiovascular diseases such as hypertension and stroke. However, the impact of hypertension on the dysfunctions induced by chronic stress remains poorly understood. In this study, using LC-MS-based metabolomics, we established a chronic stress model to demonstrate the mechanisms of stress-induced hypertension. We found that 30 metabolites in chronically stressed rats were changed; of these metabolites, 7 had been upregulated, and 23 had been downregulated, including amino acids, phospholipids, carnitines and fatty acids, many of which are involved in amino acid metabolism, cell membrane injury, ATP supply and inflammation. These metabolites are engaged in dysregulated pathways and will provide a targeted approach to study the mechanism of stress-induced hypertension. PMID: 31177559 [PubMed - as supplied by publisher]
Two-Week Aflibercept or Erlotinib Administration Does Not Induce Changes in Intestinal Morphology in Male Sprague-Dawley Rats But Aflibercept Affects Serum and Urine Metabolic Profiles.
Two-Week Aflibercept or Erlotinib Administration Does Not Induce Changes in Intestinal Morphology in Male Sprague-Dawley Rats But Aflibercept Affects Serum and Urine Metabolic Profiles. Transl Oncol. 2019 Jun 06;12(8):1122-1130 Authors: Forsgård RA, Marrachelli VG, Lindén J, Frias R, Collado MC, Korpela R, Monleon D, Spillmann T, Österlund P Abstract Gastrointestinal toxicity is a frequently observed adverse event during cancer treatment with traditional chemotherapeutics. Currently, traditional chemotherapeutics are often combined with targeted biologic agents. These biologics, however, possess a distinct toxicity profile, and they may also exacerbate the adverse effects of traditional chemotherapeutics. In this study, we aimed to characterize the gastrointestinal and metabolic changes after a 2-week treatment period with aflibercept, an antiangiogenic VEGFR decoy, and with erlotinib, a tyrosine-kinase inhibitor. Male rats were treated either with aflibercept or erlotinib for 2 weeks. During the 2-week treatment period, the animals in the aflibercept group received two subcutaneous doses of 25 mg/kg aflibercept. The erlotinib group got 10 mg/kg of erlotinib by oral gavage every other day. The control groups were treated similarly but received either saline injections or oral gavage of water. Intestinal toxicity was assessed by measuring intestinal permeability and by histological analyses of intestinal tissues. Metabolic changes were measured with 1H nuclear magnetic resonance in serum and urine. Neither aflibercept nor erlotinib induced changes in intestinal permeability or intestinal tissue morphology. However, aflibercept treatment resulted in stunted body weight gain and altered choline, amino acid, and lipid metabolism. Two-week treatment with aflibercept or erlotinib alone does not induce observable changes in gastrointestinal morphology and function. However, observed aflibercept-treatment related metabolic changes suggest alterations in intestinal microbiota, nutrient intake, and adipose tissue function. The metabolic changes are also interesting in respect to the systemic effects of aflibercept and their possible associations with adverse events caused by aflibercept administration. PMID: 31176994 [PubMed - as supplied by publisher]
Metabolomics, stunting and neurodevelopment.
Metabolomics, stunting and neurodevelopment. EBioMedicine. 2019 Jun 05;: Authors: van den Heuvel M PMID: 31176679 [PubMed - as supplied by publisher]
Mortality, growth and metabolic responses by 1H-NMR-based metabolomics of earthworms to sodium selenite exposure in soils.
Mortality, growth and metabolic responses by 1H-NMR-based metabolomics of earthworms to sodium selenite exposure in soils. Ecotoxicol Environ Saf. 2019 Jun 04;181:69-77 Authors: Shao X, He J, Liang R, Lu Y, Shi Y, Wang Y, Zheng X, Zhang S, Wang T Abstract The rapid development of selenium-enriched agriculture leads to the accumulation of selenium in the soil, which has an adverse impact on terrestrial ecosystems. In the present study, the mortality, growth inhibition rate and metabolism of earthworms were examined to investigate the toxicological effects of sodium selenite (Na2SeO3) on earthworms (Eisenia fetida) after exposuring for 14 days (d). We used 1H-NMR-based metabolomics to identify sensitive biomarkers and explored the metabolic responses of earthworms exposed to Na2SeO3. The mortality and growth inhibition rate of earthworms exposed to 70 and 90 mg/kg Na2SeO3 were significantly higher than the rate of control group. The LC50 (the median lethal concentration) of Na2SeO3 was 57.4 mg/kg in this artificial soil test of E. fetida exposed to Na2SeO3 for 14 d. However, there was no significant differences when earthworms were exposed to different concentrations of Na2SeO3. The selected metabolic markers were ATP, lactic acid, leucine, alanine, valine, glycine, glutamic acid, lysine, α-glucose and betaine. Na2SeO3 affected the metabolic level of earthworms, as the percentage of metabolic markers in the earthworm changes when exposed to different concentrations of Na2SeO3. The metabolic disturbances were greater with increasing concentrations of Na2SeO3. The differential metabolic markers were significantly changed when exposed to Na2SeO3 comparing to those in the control group, affecting the tricarboxylic acid cycle process and breaking the metabolic balance. This study showed that Na2SeO3 had toxic effect on the growth and development of earthworms. In addition, this study provided a biochemical insights for the development of selenium-enriched agriculture. PMID: 31176249 [PubMed - as supplied by publisher]
Extreme, but not moderate climate scenarios, impart sublethal effects on polyps of the Irukandji jellyfish, Carukia barnesi.
Extreme, but not moderate climate scenarios, impart sublethal effects on polyps of the Irukandji jellyfish, Carukia barnesi. Sci Total Environ. 2019 May 30;685:471-479 Authors: Boco SR, Pitt KA, Melvin SD Abstract Ocean acidification and warming, fueled by excess atmospheric carbon dioxide, can impose stress on marine organisms. Most studies testing the effects of climate change on marine organisms, however, use extreme climate projection scenarios, despite moderate projections scenarios being most likely to occur. Here, we examined the interactive effects of warming and acidification on reproduction, respiration, mobility and metabolic composition of polyps of the Irukandji jellyfish, Carukia barnesi, to determine the responses of a cubozoan jellyfish to moderate and extreme climate scenarios in Queensland, Australia. The experiment consisted two orthogonal factors: temperature (current 25 °C and future 28 °C) and pH (current (8.0) moderate (7.9) and extreme (7.7)). All polyps survived in the experiment but fewer polyps were produced in the pH 7.7 treatment compared to pH 7.9 and pH 8.0. Respiration rates were elevated in the lowest pH treatment throughout most of the experiment and polyps were approximately half as mobile in this treatment compared to pH 7.9 and pH 8.0, regardless of temperature. We identified metabolites occurring at significantly lower relative abundance in the lowest pH (i.e. glutamate, acetate, betaine, methylguanidine, lysine, sarcosine, glycine) and elevated temperature (i.e. proline, trigonelline, creatinine, mannose, acetate, betaine, methylguanidine, lysine, sarcosine) treatments. Glycine was the only metabolite exhibiting an interactive effect between pH and temperature. Our results suggest that C. barnesi polyps are unaffected by the most optimistic climate scenario and may tolerate even extreme climate conditions to some extent. PMID: 31176232 [PubMed - as supplied by publisher]
Comparing the disrupting effects of short-, medium- and long-chain chlorinated Paraffins on cell viability and metabolism.
Comparing the disrupting effects of short-, medium- and long-chain chlorinated Paraffins on cell viability and metabolism. Sci Total Environ. 2019 May 30;685:297-307 Authors: Ren X, Geng N, Zhang H, Wang F, Gong Y, Song X, Luo Y, Zhang B, Chen J Abstract With the phasing out of short-chain chlorinated paraffins (SCCPs), the production and emissions of medium- and long-chain chlorinated paraffins (MCCPs and LCCPs) are expected to increase. In this study, cell viability assay and pseudotargeted metabolomics approach were adopted to define and compare the toxic effects induced by SCCPs, MCCPs and LCCPs. The dose response curves indicated that three CP mixtures with comparable chlorine contents produced similar inhibitory effects on cell viability. At exposure concentration of 100 μg/L, three CP mixtures all induced significant increases in levels of reactive oxygen species (ROS) and malondialdehyde (MDA) and a significant reduction in level of adenosine triphosphate production (ATP), and produced similar impact intensities on overall metabolism. A stronger perturbation in phospholipid and fatty acid metabolism was observed in all CP exposure groups. In comparison with SCCPs and MCCPs, LCCPs produced a stronger suppressive effect on amino acid transport across cell membrane and induced an opposite effect on purine metabolism. Furthermore, the toxicity mechanism and possible health risks of the three types of CPs were discussed. MCCPs shared the most similar cytotoxicity and metabolic perturbation with SCCPs, suggesting that there should be concern about using MCCPs as alternatives to SCCPs. PMID: 31176216 [PubMed - as supplied by publisher]
Lactulose: patient- and dose-dependent prebiotic properties in humans.
Lactulose: patient- and dose-dependent prebiotic properties in humans. Anaerobe. 2019 Jun 05;: Authors: Jakub R, Jacek M W Abstract Lactulose is a disaccharide used in clinical practice since 1957 and has since been tested in the treatment of many human disorders, including chronic constipation, hepatic encephalopathy, and chronic kidney disease. Its mode of action is based on the lactulose fermentation by intestinal microbiota. Based on in silico, in vitro and in vivo studies we comprehensively review here the impact of lactulose on human gut/fecal and vaginal microbiota composition and both fecal and blood metabolomes. However, both in vitro and in vivo studies summarized in this review have revealed that the effects of lactulose on human microbiota composition are both patient- and dose-dependent. This highlights the need of heterogeneity indication in clinical trials. PMID: 31176002 [PubMed - as supplied by publisher]
Whole-genome sequence of Arthrinium phaeospermum, a globally distributed pathogenic fungus.
Whole-genome sequence of Arthrinium phaeospermum, a globally distributed pathogenic fungus. Genomics. 2019 Jun 05;: Authors: Li S, Tang Y, Fang X, Qiao T, Han S, Zhu T Abstract Arthrinium phaeospermum (Corda) M.B. Ellis is a globally distributed pathogenic fungus with a wide host range; its hosts include not only plants, but also humans and animals. This study aimed to develop genomic resources for A. phaeospermum to provide solid data and a theoretical basis for further studies of its pathogenesis, transcriptomics, proteomics, metabolomics and RNA genomics. The genome was obtained from the mycelia of the strain AP-Z13 using a combination of analyses with the high-throughput Illumina HiSeq 4000 system and PacBio RSII LongRead sequencing platform. Functional annotation was performed by BLASTing protein sequences against those in different publicly available databases to obtain their corresponding annotations. The genome is 48.45 Mb in size, with an N90 scaffold size of 1,931,147 bp, and encodes 19,836 putative predicted genes. This is the first report of the genome-scale assembly and annotation for A. phaeospermum, the first species in the genus Arthrinium to be subjected to whole genome sequencing. PMID: 31175977 [PubMed - as supplied by publisher]
Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses.
Related Articles Metabolomics of Thrips Resistance in Pepper (Capsicum spp.) Reveals Monomer and Dimer Acyclic Diterpene Glycosides as Potential Chemical Defenses. J Chem Ecol. 2019 Jun 08;: Authors: Macel M, Visschers IGS, Peters JL, Kappers IF, de Vos RCH, van Dam NM Abstract The development of pesticide resistance in insects and recent bans on pesticides call for the identification of natural sources of resistance in crops. Here, we used natural variation in pepper (Capsicum spp.) resistance combined with an untargeted metabolomics approach to detect secondary metabolites related to thrips (Frankliniella occidentalis) resistance. Using leaf disc choice assays, we tested 11 Capsicum accessions of C. annuum and C. chinense in both vegetative and flowering stages for thrips resistance. Metabolites in the leaves of these 11 accessions were analyzed using LC-MS based untargeted metabolomics. The choice assays showed significant differences among the accessions in thrips feeding damage. The level of resistance depended on plant developmental stage. Metabolomics analyses showed differences in metabolomes among the Capsicum species and plant developmental stages. Moreover, metabolomic profiles of resistant and susceptible accessions differed. Monomer and dimer acyclic diterpene glycosides (capsianosides) were pinpointed as metabolites that were related to thrips resistance. Sucrose and malonylated flavone glycosides were related to susceptibility. To our knowledge, this is the first time that dimer capsianosides of pepper have been linked to insect resistance. Our results show the potential of untargeted metabolomics as a tool for discovering metabolites that are important in plant - insect interactions. PMID: 31175497 [PubMed - as supplied by publisher]
Metabolomic study of the soybean pastes fermented by the single species Penicillium glabrum GQ1-3 and Aspergillus oryzae HGPA20.
Related Articles Metabolomic study of the soybean pastes fermented by the single species Penicillium glabrum GQ1-3 and Aspergillus oryzae HGPA20. Food Chem. 2019 Oct 15;295:622-629 Authors: Sun X, Lyu G, Luan Y, Yang H, Zhao Z Abstract Penicillium glabrum GQ1-3 and Aspergillus oryzae HGPA20 isolated from home-made soybean pastes were separately inoculated into soybean paste subjected to brine fermentation for 90 days. The amino acid nitrogen contents of the two fermentation systems were detected every 10 days, and both reached the maximum level at 40 days. The samples fermented for 40 days were analyzed via gas chromatography-time of flight mass spectrometry. Using univariate, multivariate and KEGG analyses, 72 differential metabolites were obtained, and 7 metabolic pathways closely related to fermentation were screened. The relative contents of 2-oxoglutarate, ornithine, glutamine, and citrulline were higher in GQ1-3, whereas those of l-homoserine, aspartic acid, and asparagine were higher in HGPA20. These findings indicate that α-ketoglutaric acid-derived amino acid synthesis is preponderant in GQ1-3, whereas oxaloacetate-derived type is predominant in HGPA20. The different pathways of amino acid synthesis lead to the distinct nutrients and umami substances in the fermented soybean pastes. PMID: 31174804 [PubMed - in process]
In silico annotation of discriminative markers of three Zanthoxylum species using molecular network derived annotation propagation.
Related Articles In silico annotation of discriminative markers of three Zanthoxylum species using molecular network derived annotation propagation. Food Chem. 2019 Oct 15;295:368-376 Authors: Lee J, da Silva RR, Jang HS, Kim HW, Kwon YS, Kim JH, Yang H Abstract In liquid chromatography-mass spectrometry (LC-MS) metabolomics, data matrices with up to thousands of variables for each ion peak are subjected to multivariate analysis (MVA) to assess the homogeneity between samples. The large dimensions of LC/MS datasets hinder the identification of the discriminant or the metabolic markers. In the present study, the molecular network (MN) approach and two in silico annotation tools, network annotation propagation (NAP) and the hierarchical chemical classification method, ClassyFire, were used to annotate the metabolites of three Zanthoxylum species, Z. bungeanum, Z. schinifolium and Z. piperitum. The in silico annotation results of the MN nodes and the MVA variables were combined and visualized in loading plots. This approach helped intuitive detection of the variables that greatly contributed to the separation of the samples in the score plot as discriminant or metabolic markers, thereby allowing rapid annotation of two flavanone derivatives. PMID: 31174771 [PubMed - in process]
Metabolomic studies as a tool for determining the post-mortem interval (PMI) in stillborn calves.
Related Articles Metabolomic studies as a tool for determining the post-mortem interval (PMI) in stillborn calves. BMC Vet Res. 2019 Jun 07;15(1):189 Authors: Jawor P, Ząbek A, Wojtowicz W, Król D, Stefaniak T, Młynarz P Abstract BACKGROUND: Perinatal mortality may vary between herds, but the cost of deaths are always higher than value of the calf. When diagnosing the cause of a calf's death it is important to determine when it occurred, before or after calving. Metabolomics is widely used to identify many human diseases, but quite rarely applied in veterinary science. The aim of this study was to compare the metabolic profiles of calves with different times of death and those of calves born alive. Into the study, twenty one healthy controls (singleton, normal assisted calving, born alive) and 75 stillborn (SB) calves (with a gestation length of ≥260 days, SB, or dead within 6 h of birth) were enrolled. Plasma and urine from SB and control calves were investigated by proton nuclear magnetic resonance based metabolomic methods. SB calves were divided into four PMI groups. One PMI group included calves that died after calving and the other groups - three comprised in utero deaths, based on pathophysiological changes (lung inflation, autolysis in internal organs, hemoglobin imbibition in the pleura and aortic arch). Partial Least Squares - Discriminant Analysis models based on plasma metabolites were calculated, reflecting assumed data clustering. RESULTS: Twenty six metabolites in plasma and 29 in urine changed significantly with PMI according to one way analysis of variance. Half the metabolites in plasma and the majority in urine increased with PMI. Six metabolites increased simultaneously in plasma and urine: acetate, sn-glycero-3-phosphocholine (GPC), leucine, valine, creatine, and alanine. CONCLUSIONS: Post-mortem changes in calves were associated with molecular variations in blood plasma and urine, showing the greatest differences for the group in which the post-mortem pathological changes were the most advanced. The results of the study show that evaluation of calf plasma or urine may be used as a diagnostic method for the determination of the PMI. Moreover, the metabolites, which unambiguously increased or decreased, can be used as potential biomarkers of PMI. PMID: 31174528 [PubMed - in process]
Translational Metabolomics: Current Challenges and Future Opportunities.
Related Articles Translational Metabolomics: Current Challenges and Future Opportunities. Metabolites. 2019 Jun 06;9(6): Authors: Pinu FR, Goldansaz SA, Jaine J Abstract Metabolomics is one of the latest omics technologies that has been applied successfully in many areas of life sciences. Despite being relatively new, a plethora of publications over the years have exploited the opportunities provided through this data and question driven approach. Most importantly, metabolomics studies have produced great breakthroughs in biomarker discovery, identification of novel metabolites and more detailed characterisation of biological pathways in many organisms. However, translation of the research outcomes into clinical tests and user-friendly interfaces has been hindered due to many factors, some of which have been outlined hereafter. This position paper is the summary of discussion on translational metabolomics undertaken during a peer session of the Australian and New Zealand Metabolomics Conference (ANZMET 2018) held in Auckland, New Zealand. Here, we discuss some of the key areas in translational metabolomics including existing challenges and suggested solutions, as well as how to expand the clinical and industrial application of metabolomics. In addition, we share our perspective on how full translational capability of metabolomics research can be explored. PMID: 31174372 [PubMed]


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Steps flared skirt
Navy wool Steps flared skirt from Études featuring a fitted waist, a short length and a flared style.
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Established in 2012 and based between Paris and New York, Études operates as a collective, working across platforms ranging from menswear to publishing. Guiding this hybrid practice is a strong emphasis on experimentation and collaboration, the group's vision at once reflecting and engaging the contemporary landscape. Expect relaxed silhouettes, graphic prints and saturated colors from this boundary-pushing brand.
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Copyright Assignment Agreement
Budget £250-750 GBP
I have recently developed a social media platform and is keen to transfer the IP rights into one vehicle ( my limited company). This is best done by way of a formal assignment agreement as a Deed. I also need a moral rights waiver agreement drawn up so that my engineers can sign it.
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I have a question, how it is the sent? You sent to General Belgrano and Can i go to look for it there?
We proudly ship worldwide. We have manufacturers, suppliers, and vendors available from all over the world. We only have an online shop, we do not have any physical store located for the walk-in customers. You can place your order online through credit or debit cards.
How do we know which style is which
Simply select the style and it will automatically change the picture on the left of your screen. You can view the image and buy accordingly.