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We have been working on potty training with all four of the Sugar Snaps, but so far, Capri is the one who has really taken off. She is at 95% success during the day not including naps. We have even started taking her on outings with her underwear and so far, so good. She had been using a small potty seat which is great for home, but she was scared of the 'big potty' whenever I encouraged her to try it. So we purchased a child size potty seat which fits onto the adult toilet. I made several attempts to have her sit on the big potty and was met with bursts of tears and terrified cries of "down!"
One day last week, she said she needed to go potty. I asked her if she wanted to use the big potty or the little potty, fully expecting her to say "little potty." Much to my surprise, she said, "Big potty." She proudly sat on the big potty and giggled at her own success. I was ecstatic too at her accomplishment and called Greg who was on his way home from work while she was still on the potty. As soon as he said "hello" I told him enthusiastically that his daughter was sitting on the big potty for the very first time. I then put the phone up to Capri's ear and she announced, "big potty" into the phone. I was expecting to hear Greg's enthusiasm as she told him the news, but he only said "hello" several times. I took the phone and asked him why he did not congratulate his daughter on using the big potty. A male voice said, "Hello? Who is this?." I said, "I'm very sorry, wrong number" and hung up the phone, a little mortified that I had shared Capri's big potty success with an unsuspecting stranger.
Do you have any potty training stories or embarrassing moments to share? I'd love to hear from you. As always, comments are welcome.


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Facebook announced Portal and Portal+, two new video specialized gadgets for the home that significantly change the manner in which we stay in contact. With a 10-inch 1280 x 800 display, Portal from Facebook enhances video calling and gives you a chance to appreciate quality time with family and companions — hands and diversion free.
Credit: Facebook

subset: commoncrawl


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StreetFX VR38 Toyota 86 Motive DVD "In The Build" Video
Syndicated content from MotiveDVD
Posted on October 5, 2016 by Andrew Hawkins
Incase you hadn't noticed,our friends at StreetFX Motorsports and Graphics have been blowing up the internet with their VR38 powered Toyota 86 built by GT Auto Garage. It's one of the craziest builds out there and has been happening alongside our VR38 powered R34 GT-R, Erubisu, build. The Albins 6-Speed sequential and Direct Clutch Services clutch was designed for both cars together. The 86 is rear wheel drive though, but will be shooting for over 1000hp as well. We caught up with Mark Trueno to have a chat and a closer look.


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General purpose seating foam (N27-160)
Medium to Firm density seating Foam
High end luxury cushion/bedding Foam
Firm seating foam ideal for commercial applications or high load requirements.
Soft foam ideal for use as a backrest foam or similar application
High impact foam ideal for any application which requires a grunty foam
This is a very dense foam perfect for any application which requires rigidity.
Please contact us about our foam cutting service


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ECON 214 Christian Ethics Project Four
ECON 214 Christian Ethics Project One
ECON 214 Christian Ethics Project Three
ECON 214 Christian Ethics Project Two
1. From a Christian perspective, why did Marxist communism fail?
1. Which is a more Christian form of government, democratic capitalism or democratic socialism?
ECON 214 Module 1 Problem Set 1
CPI of 2010 = 218.1
Equivalent value for 2010
= Original value * CPI of 2010 / CPI of original year.
ECON 214 Module 2 Exam 1
Other things the same, a decrease in the price level makes the dollars people hold worth
During periods of high and variable inflation, which of the following is unlikely to occur?
Which of the following groups...
ECON 214 Module 2 Problem Set 2
ECON 214 Module 3 Problem Set 3
ECON 214 Module 5 Problem Set 4
What determines whether a financial asset is included in the M1 money supply? Why are interest-earning checkable deposits included in M1, whereas interest-earning savings accounts and Treasury bills are not?
ECON 214 Module 6 Problem Set 5
What impact will an unanticipated increase in the money supply have on the real interest rate, real output, and employment in the short run? How will expansionary monetary policy affect these factors in the long run?...
ECON 214 Module 7 Problem Set 6
What impact do natural resources have on economic growth? Will it be possible for a country with few natural resources to grow rapidly? Why or why not.
Suppose you have just been appointed to a high level position in the...


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"My Road" with Ryan and Sarah Pettit
From TR Films A year ago
Ryan and Sarah Pettit share the amazing story of how God laid upon their hearts to adopt children...but the journey would not be easy. Facing numerous hurdles and hardships, God was faithful and led them through each struggle one step at a time.
- Tags


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The Christchurch Youth Service office has moved!
We are excited to say that we have left our space at 601 Gloucester Street and are now in a new office space at the following address along with a new office phone number.
Unit 1, 433 St. Asaph Street
Philipstown 8013
New office ph: 03 365 0341
If you would like to read more about what Christchurch Youth Services provides, click here!


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Wednesday, March 24, 2010
If you live in Hyderabad...
When two former (I am assuming Roy is all sobered up and boring now) alcoholics come together and both are bald, fit and party animals...things are bound to get a little crazy right? Hersch was the Charger's entertainer last year in Saffaland. But that doesn't mean he isn't their entertainer now. He might not know all the go to places but that doesn't mean that once he is there, he isn't unleashing his inner bad boy.
And it helps that Roy is there. He is probably 'luring' him into all sorts of drinking games:
Drink when the officials have asked you not to and get away with it.
Drink and race to the nearest bar.
Drink, call your ex-wife and try to win her back.
Drink till VB and Castle Lager send you free alcohol.
Drink your careers down the toilet...first one to the sewage gets a free beer!
Why would Deccan put two ex-boozers in the same team? Methinks, they are also sharing a room. Just so they can drink some more.
Roy's international career is done and dusted. Hersch, he may yet play in some ODI's.
Actually, if you live in Hyderabad do me this favour. Smack Hersch on the head and give Roy a monkey to drink with. After all, drunks deserve to be happy too.
6 comments:
- Mock Wah said...
wow, incidently lot of media-walas in Ind hv been behind Gibbsy with his "rehab" and sober image. Gibbs has been straight to the point in all cases, he is not reformed, transformed, ... ummm.. crap! I bet Roy is behind this. Ur right will keep an eye on the two when they are in my zone.
- March 24, 2010 at 7:57 AM
- Rayden said...
Unfortunately for Deccan, they dont have a single game in Hyderabad due to security issues. They have no home support this year. All their home games are to be played in Navi Mumbai and Cuttuck. So Roy and Gibbs need to find places to go to in these two cities.
- March 24, 2010 at 8:56 AM
- Purna said...
Thanks Mock! To be honest though, Gibbsy has been doing very well. But that Roy is not a very good influence I've heard.
Ray, oh! Well they are doing really well in that case! And I am pretty sure it's not very hard to find places to party in Mumabi!
- March 24, 2010 at 2:01 PM
- Aditya said...
To add to what Rayden said: they have 3 matches in Nagpur as well and none in Hyderabad! ( 2 in Navi Mumbai and 2 in Cuttack). 7 "Home" games!
Have you seen the post match IPL party pics Purna? Modi is making sure they have some!
- March 27, 2010 at 4:44 AM
- Purna said...
No!! Where can I find them???
- March 27, 2010 at 5:14 PM
- Aditya said...
They have these parties after the matches, they are sponsored thing, they have fashion shows and what not!
Modi is making sure they are people like Symo and Gibbsy don't miss out on the Fun!
Here is a link to the pics of the Party after RR vs DC
You can find party pics of all of them in that website!
- March 28, 2010 at 4:12 AM


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The French grant ANR SINGSTAR ("C^*-algebras and Analysis on Singular Manifolds"), is organizing a conference named
Index Theory and Singular Structures
to be held in the Mathematics institute of Toulouse, France, from Monday 29 may 2017 to Friday 2 june 2017.
The plan of the conference is to gather specialists from different horizons related to index theory understood in a broad sense (cohomological and analytical methods, secondary invariants, K-theory, C^*-algebras, groupoids, applications in geometry, analysis and topology).
We believe that this conference will be a great opportunity to realize a large state of the art in index theory and its main related fields, to bring out the next trends, to learn from each other and to create new interactions.
The conference will take place in Toulouse, also known as "La ville Rose", which is besides a beautiful french south-west city placed at the center of an extremely nice and interesting region (Midi-Pyrénées and Languedoc).
Talks will take place at the AMPHI SCHWARTZ Bat 1R3
Institut de Mathématiques de Toulouse
Campus plan available at the "practical information" in this webpage or when you get out from the Métro at the entrance of the campus.


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"A new command I give you: Love one another. As I have loved you, so you must love one another. 35 By this everyone will know that you are my disciples, if you love one another." John 13:34-35
This is a command. Jesus knows He's leaving. The standard of how we are to love each other is how Jesus loves us.
"Therefore, I urge you, brothers and sisters, in view of God's mercy, to offer your bodies as a living sacrifice, holy and pleasing to God—this is your true and proper worship. 2 Do not conform to the pattern of this world, but be transformed by the renewing of your mind. Then you will be able to test and approve what God's will is—his good, pleasing and perfect will." Romans 12:1-2
This is what we're here for.
Live with the intent of glorifying God.
Do not conform to the pattern of this world (world thought).
Very rarely do you see good news in the news.
It's very easy to slide into a world view.
Satan loves to stifle our witness.
Transformation is the same word used for a caterpillar becoming a butterfly. Two distinct creatures.
"For by the grace given me I say to every one of you: Do not think of yourself more highly than you ought, but rather think of yourself with sober judgment, in accordance with the faith God has distributed to each of you." Romans 12:3
When we judge people we're missing the point. Stay humble.
We don't deserve to be here either. My testimony should be look where I was and look where God has brought me.
Each of us has a role to play.
"We have different gifts, according to the grace given to each of us. If your gift is prophesying, then prophesy in accordance with your[a] faith; 7 if it is serving, then serve; if it is teaching, then teach; 8 if it is to encourage, then give encouragement; if it is giving, then give generously; if it is to lead,[b] do it diligently; if it is to show mercy, do it cheerfully." Romans 12:6-8
Have you figured out your role?
All are efforts of love.
Be a living sacrifice.
It takes sacrifice to serve, to teach, to lead, to give.
Teachable moments -- openings.
Making most of the gift by making it who you are.
I'm a Jesus Follower!
Examine ourselves to know who we really are. Where we are.
What is lacking? Self evaluate.
It is easy to coast in our faith.
God wants us to participate.
"Love must be sincere. Hate what is evil; cling to what is good. 10 Be devoted to one another in love. Honor one another above yourselves." Romans 12:9-10
Our faith can't be just like "how ya doing?"
Church meant to be a growing community. If it's not growing it's dead.
Be devoted to one another.
Look for opportunities.
Fail forward. Learn from you failings.
Self evaluation isn't comfortable but it's necessary.
"Never be lacking in zeal, but keep your spiritual fervor, serving the Lord." Romans 12:11
What's it going to take to get us excited about Jesus?
"Be joyful in hope, patient in affliction, faithful in prayer. 13 Share with the Lord's people who are in need. Practice hospitality." Romans 12:12-13
These are not suggestions.
"Bless those who persecute you; bless and do not curse. 15 Rejoice with those who rejoice; mourn with those who mourn. 16 Live in harmony with one another.Do not be proud, but be willing to associate with people of low position.[a] Do not be conceited." Romans 12:14-16
People need you to be with them. They need a Halleluia from you. They need you to be with them emotionally.
"Do not repay anyone evil for evil. Be careful to do what is right in the eyes of everyone. 18 If it is possible, as far as it depends on you, live at peace with everyone." Romans 12:17-18
Where are you on the scale of love?
4 "Love is patient, love is kind. It does not envy, it does not boast, it is not proud.5 It does not dishonor others, it is not self-seeking, it is not easily angered, it keeps no record of wrongs. 6 Love does not delight in evil but rejoices with the truth. 7 It always protects, always trusts, always hopes, always perseveres.
8 Love never fails. But where there are prophecies, they will cease; where there are tongues, they will be stilled; where there is knowledge, it will pass away." 1 Corinthians 13:4-8


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Is it time for your annual MOT test? Want to make sure you're getting MOT testing at a government approved facility in Oldham? Let Hollinwood Tyre, Exhaust, MOT & Service Station help you accomplish that at our garage. You can get your MOT test carried out here with just a quick phone call. We'll book you in and the MOT testing will be carried out as you wait, saving you time and covering any concerns you have with your car.
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Make sure your car is legally safe to drive around in by booking your MOT test on:


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Liberty Legal PLLC: (603) 583-4444
In our blog we will try and give updates on the law and cases throughout New Hampshire, as well as interesting events that occur throughout the country.
The statute reads:
I. Any person held in official custody who commits an assault under RSA 631 is guilty of an offense under this section
II. Aninmate is guilty of aggravated assault on a corrections staff member when, with intent to harass, threaten, or alarm a person whom the inmate knows or reasoanbly should know to be an employee of such facility, or an employee of any law enforcement agency, the inmate causes or attempts to cause such employee to come in contact with blood, seminal fluid, urine, or feces by throwing or expelling such fluid or material.
The statute then goes on to define terms in the body of the paragraphs I and II. Make no mistake, Assault by Prisoners is a serious offense and when a police officer and other employee is involved this is a felony charge.
Manchester Office
212 Coolidge Ave.
Manchester, NH 03102
(603) 583-4444
Disclaimer: This website may be considered advertising. Use of this website does not form an attorney/client relationship. An Attorney/client relationship is formed only after an agreement is entered into between an Attorney of Liberty Legal and a client is reached. Past results do not guarentee future results. Each case and each client have unique circumstances.
I will allow images of myself on this website to be used for other publications including those involving commercial uses. I reserve the right to revoke granting permission and may claim copyright to the images at my own discretion.


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Kara and I have declared today "Knitting and a Saga" day. This means we will knit and watch a saga together. Could that sound any more fun?
I define a saga as any movie with more than one DVD. And I love them. Today we are watching Jewel in the Crown which is a pretty old mini-series that neither of us have seen. But it is set in India and there are British people in it so I don't know how it can go wrong. The saga is 13 episodes at 50 minutes each and so we may not get through it all today as we aren't getting started until around 4 or so but we sure will try.
As for what I am knitting, I have started a new project. I was looking for a new sweater to knit and couldn't settle on anything. I just wasn't feeling a project enough to drop money on yarn and commit to it. Then, in a moment of clarity, I realized that last Spring I bought the yarn and pattern for a project that I never started. I loved the sweater but as soon as I saw that it required tiny needles I held off. Of course, I have knit several sweaters since with really small needles and learned it is no big thing. I like the tiny needled sweaters. It is my thing.
So I cast on for this beauty:
Oh I like this sweater. It is from Phildar Tendances, Spring 2005. Phildar is really bad at naming their projects so this one is just Zip Cardigan. I am actually using the same yarn (color and all) for mine and am LOVING the yarn. This is how it is knitting up so far…
And yes, that is the handle of my camera I brilliantly got in the shot.
It might seem like I am taking a picture of the WS but the sweater is knit in reverse stockingette. It throws me off a little as it seems counter-intuitive but I think in the end I will like it. I am finished knitting the back and am casting on for both fronts today (I will knit them at same time.) The only hesitation I have about this sweater is the zipper–specifically the fact that it needs to be sewn in. I guess this is why I have friends…right Ann?
Next up is a finished Glee. I promise. Happy President's Day everybody!
The following 14 comments were originally left at blogspirit:
Knitting & a saga sounds like a great way to spend the day! 🙂 I've done knitting & scrapbooking days like that – Alias & Wonder Woman were good for that. 🙂 Have fun! The new sweater project looks like it will be great.
i actually started this cardigan just over 12 months ago and got the back and most of the bands finished. since then it has stalled big time so i am actually considering restarting it but in debbie bliss baby cashmerino from stash. i can't wait to see how yours turns out.
That is a cute sweater! I'm all for knitting and a saga. I don't have any sagas to watch, but a few videos and as M is gone for a few hours, uninterrupted knitting time is here for me! Yay! Enjoy your day.
Margene can help you–I've seen her do it!
Knitting and a Saga sounds like a wonderful tradition.
I love that mossy green color! Knitting and a Saga sounds great too, around here we have weekends of Knitting and a Video Game. Not quite as great.
I can't wait to help you put in the zipper ~and start my next knit ~this week?
Love the new sweater; I love the really long arm ribbing. I, too, am a small needle sweater kinda girl. And Knitting and a Saga sounds like such fun (as long as there's no Titanic involved!)
That sweater is going to be beautiful! Sounds like a perfect day!
What a wonderful way to spend a winter day off! So cozy.
i'm all for a good saga and a good bit of knitting time!
i like the zip cardi, what a great pattern. phildar have such cool designs and i've been lusting over some of the tendances booklets for a while now.
I LOVE Phildar designs. The cardigan you picked out is lovely. Love the lines! Reverse stockinette is fun for a change too!
knitting + saga = best idea ever
i've been watching the BBC pride and prejudice every weekend. if any of those sagas you watch are really good, give us a review on the blog! not that there's anything wrong with P&P every weekend. 🙂
that sweater is going to be SO CUTE! i can't wait to see the finished product!
Oooh, pretty! Can't wait to see how it turns out! I so wish I had time for knitting and a saga! One of these days I'm going to rent the first season of 24 and lock myself in my house all day. It's not really a saga, I guess, but it's long, and would be a good excuse not to move my butt off the couch! I'm all for not moving my butt off the couch, you know.
I'm so glad you're working on this! I think this will be the next one that I pull out of the closet of in-progress pieces. Yours is looking really fabulous 🙂


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. . . that the Maria Kaupas Center is bursting at the seams with
Anticipation, celebration, and appreciaton?
According to Pope Francis,
"Advent begins a new journey"
At the MKC, this "new journey" involves learning about the
history and traditions of Advent during Daily Reflection
. . . it also means looking outside of oneself to help others less fortunate by restocking the
empty shelves of local food pantries,
The Maria Kaupas Center was proud to partner with the Junior and Senior seminars of Catalyst-Maria High School and with the Women's Basketball team from Geneva High School in our annual Advent food drive.
And, most importantly, it means ". . .draw[ing] from the crib
the joy and deep peace that Jesus comes to bring to the world."
Tweet by Pope Francis (you just have to love a tweeting Pontiff!)
MKC volunteer and Maria High School alumna Suzy Etsch ('75)
helped middle school students create rustic nativity scenes that
eloquently capture the simple beauty of the Christ's birth in the stable.
Anticipation of another kind is an important part of each cooking class sponsored by the MKC.
Scholars can't wait until they get to eat the delicacies they create in the Kaupas Kitchen under the direction of talented adult cooks. Recently, Dr. Mary Roy and her sister, MKC Board member Nadine Roy, Holy Cross Hospital VP Dennis Ryan, and the MKC's own Matt Whalen took turns in the kitchen.
In addition to learning to make (and then eat) delicious recipes,
young cooks are learning to appreciate different foods, spices, and fresh ingredients as well as learning
kitchen etiquette and proper cooking techniques.
Celebration has also been a theme at the MKC lately!
The fall dance class celebrated with an-end-of session recital where girls demonstrated their
fancy footwork and stage presence:
Here's a Did You Know in a Did You Know!
Did you know that Pope Francis loves the tango and used to dance when he was young?
A celebration of another sort took place in
Sister Elizabeth Ann's Sacred Sewing classes. Our adult seamstresses finished and modeled their most recent project, fashionable bags (though some might say that the little guy with the pillow really steals the show) and our junior designers created Christmas runners.
Yet another kind of celebration is Praise Dance, a mini session offered to 7th grade girls by Catalyst-Maria teacher's aide Erin Bell. The girls are learning to communicate their joy in life and love of God through music and dance.
And at the MKC, a sense of appreciation always accompanies anticipation and celebration.
We are so grateful
for the gift of our talented volunteers
for the adults who come and give purpose to the MKC during the day
for the joie de vivre the scholars bring to the Center every afternoon
for our many, many benefactors.
The MKC is indebted to the following Foundations for rewarding our work with recent grants:
The Edmund and Alice Opler Foundation
Porticus North America Foundation
We are also thankful to all those individuals who supported our #GivingTuesday efforts;
thanks to your generosity (and the generous match of Nadine Roy and Peg Weiser),
we more than doubled our goal and raised
We are grateful for the opportunity to spend quality time with the people in Marquette Park
the children, the adults, and coming soon,
the senior citizens!
But mostly, we are grateful to God
for the opportunity to share the message of His
unconditional love
in an environment that strives daily to bring Gospel values to life.
To quote another of Pope Francis's tweets:
"May we always say thank you to God"


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BioCell® Collagen A natural mix: hydrolyzed collagen II, GAG, and hyaluronic acid
- 70% collagen
- 20% chondroitin sulfate (GAG)
- 10% hyaluronic acid
- Reduce joint discomfort, reduce pain, and increase collagen supply
- Reduce wrinkles and fine lines, reduce face and body skin dryness
A clinically tested naturally occuring nutritional matrix. The highest quality skin and joint health ingredient for over twenty years.
A patented dietary supplement ingredient; hydrolyzed collagen type II (70%), chondroitin sulfate (20%), and low weight hyaluronic acid (10%). Repeatedly tested in double blind, placebo-controlled trials, BioCell Collagen has proven to promote muscle recovery, reduce joint pain and discomfort, improve wrinkle depth and appearance, help dry skin, revivify dermal collagen content, dermal hemoglobin content, and general skin health and appearance in people.
BioCell Collagen is extracted from the sternal cartilage of hormone free and antibiotic free chickens. Cartilage that is very similar to human's articular cartilage, and provides an ample source of hydrolyzed collagen type II, hyaluronic acid, and chondroitin sulfate. Chicken sternal cartilage is a clean and pure source since it does not contain blood or have any blood supply, capillaries, and lymphatic vessels in the cartilage. Type II collagen naturally co-exists with chondroitin sulfate and hyaluronic acid in the blood-free sternal cartilage of chickens, a safer, cleaner, and rarer derivative than all the other collagen types and supplement ingredients. BioCell Collagen is the highest quality collagen available, and in a dynamic formula it has been clinically tested five times in human trials and has proven its considerable capacity as a functional, bioavailable, and safe supplement ingredient.
BioCell Collagen is made in a patented procedure; filtration, purification, concentration, hydrolysis, and sterilization (Bio-Optimization™). Show more...


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Who We Are
Our History
Our Culture
Our Core Values
Our Team
Real Estate Cleaning
Residential / Domestic
Office & Commercial
Strata Title & Body Corporate
71-Point Checklist
High Pressure Cleaning
Deck Coating
Rates & TCs
Products & Tips
Gift Certificates
08 8928 0872
0439 303 678
High Pressure Cleaning
End of Lease & Spring clean
Strata Title & Common Area
Property Services
Window Cleaning
Deck & Timber Coating
About Us
We Specialise in Cleaning and Decking Coating Services And Preparing Homes For Sale And For Rent.
Attention To Detail Darwin Cleaning is a locally owned and operated family business that has been providing a high quality cleaning service to Darwin, Palmerston and surrounding areas since 2004. We cater to households, business and corporate clients who require a clean home or workplace for themselves and their employees or a maintenance program for timber decks, railings and steps.
Attention to Detail Darwin Cleaning & Deck Coating Darwin are the premium deck coating and cleaning service, offering an efficient, extremely high standard, honest and reliable service. Our clients include a range of business, Real Estate Agents, Property Managers, Body Corporate and Home Owners. We specialise in commercial and residential deck coating and preparing homes for sale or rent. Every year, we perform more than 2000 services for clients who take advantage of our expert commercial and residential
deck coating
cleaning services
. We organise and provide regular deck coating and cleaning for more than 200 properties, including offices, recreation centres, holiday stays and residential properties.
Attention to Detail Darwin Cleaning has developed a deck coating and cleaning system using eco-friendly products to avoid any potential side effects from exposure to harmful detergent and chemical residues.
We look after professionals, working families, retirees and individuals looking for a trusted brand to provide relief from deck maintenance and household work.
Attention to Detail Darwin Cleaning is a company built on the following four pillars:
Who We Are
Our History
Our Culture
Our Core Values
Our Team
Our History
From humble beginnings, Attention to Detail Darwin Cleaning has grown to become a very successful deck coating and cleaning business in Darwin, Palmerston and beyond.
In 2000, local resident Brenda Bagshaw left her position with a civil engineering company in Darwin to start a family. By 2003, Brenda was at home with two young children and no income. Always a diligent cleaner, friends often commented on the quality of Brenda's cleaning work - with many joking they'd love her to clean their home.
After attending an
demonstration about using environmentally friendly micro fibre cleaning products that are a healthier way to clean, Brenda became a consultant. Often during ENJO demonstrations people were so impressed with her cleaning they asked if she was available to clean their homes.
House cleaning was the entry point into the cleaning industry and Attention to Detail Darwin Cleaning quickly became an in-demand Darwin cleaning and deck coating service.
Brenda continues to be the visionary, driving force behind the continued success of Attention to Detail Darwin Cleaning & Deck Coating Darwin. Her ultimate goal is to provide a leading-edge deck coating and cleaning business with a dedicated team that make the extra effort to provide 100% customer satisfaction.
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Our culture here at Attention to Detail Darwin Cleaning is to provide an efficient consistent service to ensure you are 100% satisfied every time. We offer a set and forget deck coating and cleaning maintenance service to businesses and homes alike.
Our team of specialists love their jobs and take great pride in ensuring your business, property or home receives the ultimate service. The team have all been selected for their honest, conscientious and hardworking strengths. We have a strong team culture and participate in and enjoy team bonding activities. This creates a caring and considerate ethos for our team and clients alike, resulting in the employment of long term team members which is added security for your home or business.
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We provide premium quality service. Our brand is high quality only.
A prerequisite for joining Attention to Detail Darwin Cleaning team is honesty and personal principles. Integrity is essential and a core quality of all members of our team.
A strong team environment ensures a reliable service for all of our clients.
Testament to being able to maintain our high standards over a long period would be that we still service our original clients.
Our Team
Owner operator Brenda Bagshaw is the visionary and driving force behind providing leading edge deck coating and cleaning services that are synonymous with quality. The specialist team are trained to meet high level standards and go the extra mile to provide 100% customer satisfaction. Client feedback is valued and assists us to incorporate improvements into daily operations.
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As well as offering an excellent service to clients, Attention to Detail Darwin Cleaning provides a great opportunity for enthusiastic people who are dedicated to working hard, well presented and reliable with a professional attitude. We are always on the lookout for people who take pride in their work and show up on time, every time. Becoming a cleaning and coating specialist is ideal for people looking for extra income together with a built-in exercise program!
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"Fast. Friendly. Fabulous!"


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This course offers an introduction to the issue of anxiety among teenagers and young people. It is aimed at health professionals and teachers without specialist training in this area, and does not require any prior experience or knowledge. The course will help them to identify anxiety disorders experienced by young people, understand 'stigma' related to mental health and develop their ability to help a teenager experiencing debilitating anxiety.
It is taught over 3 hours a week for a length of 3 weeks, each covering a different aspect of addressing anxiety and mental health among young people. It will be lead by an experienced adult and youth mental health nurse clinician and lecturer.


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tag:blogger.com,1999:blog-16879994.post2033602307862223820..comments2020-07-01T08:51:39.211-04:00Comments on Local Ecologist: Then & Now: Showy Callery firstname.lastname@example.com:blogger.com,1999:blog-16879994.post-58661544942125482602012-02-03T18:19:44.058-05:002012-02-03T18:19:44.058-05:00Lauren: thank you for informative comment. Would ...Lauren: thank you for informative comment. Would you mind providing a list of alternatives to the Calley pear? I would like publish a follow-up to this email@example.com:blogger.com,1999:blog-16879994.post-25609173719049999822012-02-01T23:59:06.432-05:002012-02-01T23:59:06.432-05:00"Another problem with the "improved"..."Another problem with the "improved" pear cultivars, is that they cross pollinate with 'Bradford' pear. 'Bradford' pear was originally introduced as a fruitless tree. Suddenly 'Bradford' and other pears began to produce large quantities of marble size fruit. This fruit was carried, probably by birds, so that now hybrid Callery pears are showing up along roadsides and in forested areas where they will out compete and displace native species."

http://web.extension.illinois.edu/cfiv/homeowners/050223.html

Callery Pears and its cultivars are planted everywhere. There are a lot of great trees, especially native trees, out there that can replace these trees. The birds are spreading the fruits to other areas. At my university located in PA, callery pears are present in a meadow along a roadside near a woodland edge. They are creeping their way into the woodland. In the fall all you see is the mass of fall color of the Callery Pear. It is not something to be praised.

Also, Koelreuteria paniculata has been found to have invasive tendencies in the Northeast as well.

BE INFORMED, KNOW WHAT YOU'RE PLANTING.laurenhttps://firstname.lastname@example.com:blogger.com,1999:blog-16879994.post-60293132318289230902011-12-06T20:34:05.197-05:002011-12-06T20:34:05.197-05:00Timely topic. I got an email today from a custome...Timely topic. I got an email today from a customer who told me her community assc. said she had to plant a Bradford to replace one that died. I encouraged her to contact the assc. to get this nasty tree off of the approved list. We are going to plant a Chanticleer in its place, without letting anyone know.Leshttps://firstname.lastname@example.com


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CoE professor wins $4.79 million nanotechnology contract
In an unusual vote of confidence, the U.S. Army has awarded CoE Professor Shubhra Gangopadhyay a $4.79 million contract to devise miniaturized devices aimed at improving American military capabilities.
Gangopadhyay, an electrical and computer engineering professor who heads the CoE's International Center for Nano/Micro Systems and Nanotechnology, will develop several new ways to combine microchip technology with nanotechnology under the contract. The three–year contract—which runs through May 2009—significantly increases the Army's financial commitment to MU's nanotechnology research, Gangopadhyay said.
"This is an extension of work I've already been doing with them for the last three years," Gangopadhyay said. "But it's broadening it a lot."
While federal funding for the new field of nanotechnology has increased dramatically over the last five years, the U.S. Department of Defense has been wary of financing multiphase nanotechnology research. Experts believe nanotechnology, which works with particles the size of just a few atoms, offers breakthrough potential for the military as well as for medical and commercial purposes.
Gangopadhyay's first project under the new Army contract calls for the development of a nanomaterial that can propel a warhead or rocket via a microchip. Other projects included in the contract would create news ways to detect explosives and new, more efficient sources of power.


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Конспект урока "Pet's hotel and Zoos"
Animal World
Тема урока: Pet's hotel and Zoos.
Ход урока.
Орг. момент: Проверить готовность уч-ся к уроку.
Приветствие: У: Good morning I'm glad to see you
Уч-ся: Good morning We are glad to see you too
У: Thank you. Please, sit down. The theme of our lesson today is "Pet's
hotels and Zoos."
Please open you books by Derevyanko at page 74 and Let's do ex№1
Основная часть: Ex№1. (Look at the pets. Lesten and their names)
аудирование текста
Ex№2. 1. Where does Tom work?
He works at a pet's hotels
2. What is a pet hotel?
It's a hotel where pets live
3. Where do you take your pet, when you go to holiday?
We take it at the pet hotel
4. How many rooms are there in this hotel?
There are 30 rooms there.
Is there a kitchen and a bathroom?
Yes, there is. There is also a play ground.
Who lives in this hotel?
Dogs, cats, fish, hamsters, budgies, guinea [gini], pigs, mice,
tortoise live there. What's the name of the hotel?
The name of the hotel is "Bow- Miaow"
У: Ok. And now lets read ex№3 a at p.74 and say what animal is it ?
Уч-ся: (читают текст)
У: What's in English for whiskers (усы). What animal is it?
Уч-ся: It's a mouse
У: Excellent. Now I want you to do Ex№14 at p 74. Fill in the gaps with
these words
(They do the ex)
My name's Brownie I am five years old. I am long and my legs are
short. But I can run very fast I don't like cats. I have got many dog
friends at the hotel. We play all day I don't miss home
У: Ex №5 p75. What does the fish say. Make up the story.
Уч-ся: Hello, my name's Bubble I'm five months old. I'm small and red. I
have a long tail and I can swim. I eat worms. I have no friends at the
hotel and I miss home.
У: What's in Russian for worms (черви).
У: Ex№6. Listen and sing.
У: Let's do ex№7. Read the words with the letter G/g. Make two
[d ] page, hedgehog, giraffe, budgie
[ g ] go, guinea pig, garden, good, gorilla
У: That's OK. Thank you. Please write down your homework in to your
diary books.
H.W. Ex 8, p.75. You must write an ad for the dog who needs a
loving friend. OK?
У: And now open your books by Vereshchagina at p.31 and let's control
the text you have read at home. Please answer my questions.
1. Where is Wendsor Safari Park?
2. When does it work?
3. What must visitors to remember in this park?
4. What sections are there in the park?
5. What can you see in a Sea world section?
6. What about the Bird world section?
Уч-ся: 1. Wendsor Safari Park is near London, in the South of England
2. It is open from 10 o'clock to 7 o'clock
3. Visitors must remember three important things: they must drive
slowly, never open the car window and never get out of the car in the
places where wild animals live free
4. There are three sections in this park a Sea world section, a Bird
world and of course an Animal world section.
5. In a Sea world section we can see dolphins, sea-lions, fish and other
sea animals.
6. In the Bird world section there are hundreds of different birds from
lots of different countries.
У: OK. Today you work brilliant . Your marks are good and excellent.
Let's play our favorite game "The stairs". You must write only
У: OK. We have 5 minutes and I want we have fun Open Cambridge
English at p.106 and lets divide the animal into groups
Flies, cats, whales, ostriches, horses, mosquitoes, salmon, dolphins,
tiger, bats, crocodiles, lions, goldfish, sharks, flying fish, parrots,
monkeys, cows, bees, penguins, rhinoceroses.
Заключение: Оценить учащихся
Домашнее задание
Английский язык - еще материалы к урокам:
- Презентация "Present Simple" 3 класс
- Конспект урока "Необитаемый остров" 8 класс
- Контрольная работа по немецкому 9 класс (к учебнику И. Л.Бим" Немецкий язык")
- Методика и технологии подготовки обучающихся к сдаче ОГЭ и ЕГЭ к разделу аудирование по английскому языку
- Презентация "My beautiful Homeland - the South Ural" 9 класс
- План-конспект урока "Моя прекрасная Родина - Южный Урал. Путешествие во времени" 9 класс


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Brian Cregan © 19 September 2013. Posted in the Magazine ( · Historical Fiction · Interviews ). This month Brian Cregans Parnell A Novel was launched by The History Press Ireland. Described by RTEs Myles Dungan as a monumental task tackled with style and aplomb, Parnell A Novel is a page turner which keeps the reader gripped, as Diarmaid Ferriter says in the Irish Independent, Cregan has produced a rollicking read that is well researched and faithful to many of the details and happenings of Parnells life and public rhetoric. Parnell A Novel makes history accessible for the many who perhaps struggled with the subject at school, adding the ingredients of great fiction to research that took almost ten years. But how do you tackle a subject like this, and make it accessible? Brian Cregan explained to writing.ie:There are various forms of historical fiction. The most common is one in which fictional characters play out their story in a particular time and place and the great historical figures of that era have only walk-on parts. Another form becoming more common in recent years is biographical fiction in which the novel centres on the events or imagined life of a real character. An interesting example of this was The Master by Colm Tobin and Author Author by David Lodge both novels about Henry James. A third form of historical fiction which Gabriel Garcia Marquez called novelised history is one in which a novel tells the story of a great historical figure, as Gore Vidal did in Lincoln A Novel.But whatever the form of fiction, the first decision one has to make in writing a novel is to decide what is the narrative device who is telling the story.One option, (used in I Claudius by Robert Graves) is to tell the story in the first person in that case a memoir of an emperor. A second device is that of shifting perspectives. Thus in Lincoln, the story is told through the eyes of numerous different characters, e.g. the Secretary of State, the Secretary of War, his personal secretary. In this way, Lincoln is viewed through the eyes of many protagonists and a rounded picture of the man emerges. This is however, unusual, difficult to achieve and somewhat disconcerting for the reader.A third option is to use the omniscient author as was done in Napoleon by Max Gallo. Here the author can see and hear what every character is doing and even know what they are thinking. This is a common way to write historical fiction because it allows the author great freedom to tell the story.A fourth and unusual device brilliantly used by John Williams in his novel Augustus is to use letters, journals and other documents written by various participants to tell the story of Octavians rise to become Emperor of Rome. There is no single narrator. The story is started by one person, carried on by another, reflected on by a third and a complicated mosaic of differing views emerge.However the choice I made in Parnell A Novel was to tell the story of Parnell through the perspective of a fictional character his secretary James Harrison. In my view, there is always something compelling about one person telling their story. Although Harrison is a reliable narrator, he is, of necessity, partisan, even prejudiced. He is not and does not claim to be objective. He has his likes and dislikes and reacts to other characters in the novel as he meets them along the way.Another advantage of telling a story in this manner is that readers quickly become familiar with the voice and point of view and can settle into the novel. The disadvantage is that Harrison is not there for the private scenes between Parnell and Katharine OShea and is unable to have access to their thoughts, or emotions unless they are expressed to him. At an early stage, I sought to write the novel in an omniscient voice. But for me, with this story, it lacked the immediacy, the colour and the personal reaction to characters which I wanted to evoke.Parnell A Novel is structured as Harrisons memoir of Parnell. Parnell was born in 1846 the second year of the Great Famine. But as a wealthy landowning family, the famine would have had little or no effect on the Parnell family. Harrison, by contrast, was also born during the famine: his mother died in that famine and he was found, as a new-born child, beside his dead mother, by a Church of Ireland clergyman and placed for adoption with a wealthy Dublin doctor and his wife. Thus Harrison is brought up and educated as a young Protestant boy. After his university education Harrison takes a position as secretary to Parnell, a position which, by virtue of his upbringing and education, he is now able to fulfil.It is only in the second part of the novel when famine threatens the country again and Harrison sees scenes of a starving and destitute people that he realises that he is of these people. With an awakening awareness of his background and the tragedy of his mothers fate, Harrison now becomes personally involved in the struggle for land reform and home rule.Parnell asks Harrison to become involved in the Land League and also to stand as a Member of Parliament. Once Harrison is elected an MP, he is now present in the House of Commons sitting beside Parnell and participating in the debates with him.Moreover Harrison, as befitted men of his upbringing and social class is also in search of a profession and eventually, after encouragement from Justin McCarthy, Harrison is called to the Bar and thus can act for Parnell in the numerous court trials in which Parnell is involved.Each narrative device has its advantages and drawbacks. Some stories lend themselves more obviously to one form of narration than another: thus a story with multiple strands or in different periods of time may require an omniscient author whereas a story of one man can be told by an eyewitness. In the end, it is a matter of choice and of instinct. How do you want to tell the story and perhaps as important how do you want readers to receive the story. In telling the story of Parnell, I wanted to consider the question: what would it have been like to have walked with Parnell, to have talked with him and to have lived through those times. The best answer to that question was, in my view, to have an eyewitness account to have Harrisons account of how Parnell achieved greatness despite the overwhelming obstacles.(c) Brian CreganAbout Parnell A NovelDublin, March 1874. Charles Stewart Parnell, only twenty-six years old, speaks in public for the first time as a candidate for Irelands Home Rule Party. Hesitant and nervous, he stumbles through his speech to the sound of booing and leaves the platform humiliated. He vows that in future he will find his voice and make it heard.Within three years of this speech, Parnell made the House of Commons unworkable; within six years he had destroyed the landlords in Ireland; and within a decade he controlled the House of Commons and put English Prime Ministers in and out of government at will.Parnell: A Novel charts the life of this most enigmatic and remarkable of men, as seen through the eyes of his loyal secretary James Harrison. From the Houses of Parliament to the blighted villages of the West of Ireland, from the courtrooms of the Royal Courts of Justice to the cells of Kilmainham Gaol, this is the story of how the character of one man could alter the fate of two nations.
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First things first, apologies for missing a couple of games. I was gone for the weekend and missed the revenge beatdown of the Celtics and a close loss to the Raptors. Ohhh well, too much preseason probably bad for your health anyway. Getting into last night's game:
We Won!!!
Now this was against the godawful, league worst 76ers playing without their best player in Michael Carter-Williams in the Syracuse arena where people cheered pretty much anytime Melo touched the ball, but still. Win's a win. A little scary seeing as a 22 point lead evaporated into about 7 in the fourth quarter against a team that essentially could not shoot 3 pointers and missed at least 4 point-blank lay-ups, but still. Win's a win. Really compensating here.
Of Note:
The Knicks started the game with a Calderon-Shumpert-Anthony-Acy-Dalembert lineup that is seriously lacking in shooting. I know Derek Fisher is trying to preach defense, but the way Acy's defenders sagged off of him when he was behind the arc was seriously upsetting. If he can become a league average shooter behind the arc, it'll go a long way to giving this lineup the necessary punch. Plus, I like Acy. He's got some great hustle when rebounding and is only 24 years old. Youth Movement!
The ball movement also started a little awkward with players just whipping the ball around the arc. If they don't enter it into the post or set a high pick and roll, their defenders are just right on them and the Knicks possess very few players who can take their man off the dribble. That being said, the ball movement became excellent as the game went on and opened up a ton of 3 pointers. People seemed reluctant taking them towards the beginning of the game, but then just started putting them up, which I like. Shump had a couple nice ones.
ENTRY PASSES!!! HOLY SHIT!! Time to work on the entry passes. We had something like 4 turnovers just lobbing it into a guy in post position and a couple more close ones. This should not be as difficult as it is.
Things I liked:
Hand-off passes from a big to a guard on a curl cut. Yes. When the cutter is someone fast like Shumpert or "Holy Shit" Shane Larkin, these guys are fast enough to get to the basket for a lay-in before anyone can foul. And when the screener can shoot, like Jason Smith, it's an easy pick-and-pop opportunity if the defense reacts. Larkin and Smith in particular showed some nice chemistry.
Dalembert continues to impress me. He had something like 3 blocks right after the half and hit his first three jumpers when everyone else on the Knicks was bricking EVERYTHING. Seriously, we were on track for a 12 point first quarter.
SHUMP!! Shumpert looked great in the second half when he started letting the 3 ball fly and started cutting more aggressively. He almost had one DAMN! play but was fouled going to the hoop. The way he flashes his athleticism makes me pissed these guys tried to trade him. Also they did a little interview segment where he claimed to be mad for a lot of last year and is now just having more fun being a 24 year old playing ball in New York. Clyde picked up on this too and broke out the rhyme "Winning and Grinning". Keep the smiles up.
I guess we do have to put everything into context, though, and say this was against a team that featured precisely one NBA caliber player in Nerlens Noel (and maaaybe a bench guy in Tony Wroten, though he had a terrible game). I remember really feeling enthusiastic about how the ball was moving and the Knicks seemed to be playing together, but I can't imagine this same performance netting a Win against a playoff team. Still, these guys have a nice long break until their next game on Monday, so rest up and keep running that triangle.


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Moldavite Drop | Czech Meteorite | Stock B
Feeling like there's a lot to deal with, here on Earth? Transcend the bs with some gorgeous moldavite
2.7 grams; just under 24mm
From Crystal Vaults:
"Moldavite is good for counteracting cynicism and connects even the most world-weary adult with the wonders of the universe. It eases away doubts, even when the cause is unknown, and calms worries about money by providing solutions not previously considered. [Eason, 284]
Carrying or wearing Moldavite in jewelry allows its energies to remain in one's vibrational field throughout the day strengthening its effects and increasing the incidents of beneficial synchronicities in daily life. Because of Moldavite's intense vibration, some may experience light-headedness or a lack of grounding and may need to acclimate themselves gradually to wearing it. [Simmons, 262-263]
Moldavite is a useful stone for star children and sensitive souls who find it difficult being in incarnation on the earth, and who cannot adjust to suffering and deep emotions. Placed on the heart, Moldavite uncovers the reasons and purpose for why one is here, and eases the "homesickness" for those whose origin is not Earth. [Hall, 188-189]
. . .
Moldavite is a powerful aid for meditation and dream-work, as well as increasing one's sensitivity to guidance, intuition and telepathy, and the ability to understand messages sent from higher realms. [Simmons, 262-263][Megemont, 126]"
Please note sizing in the description. These are small and photographed up close and enlarged, so you can see their detail. They were photographed on a 1" diameter display case (for size reference).


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Ahead of their UTS debut, Hermitude's Angus 'Elgusto' Stuart and Luke 'Dubs' Dubber chat to Greta Balog.
Angus 'Elgusto' Stuart and Luke 'Dubs' Dubber of Australian hip hop duo Hermitude didn't expect sell out shows when they announced their national tour, The Villain. And now, with only six weeks until the tour kicks off, the pair have announced even more shows around the country.
"There's been such a great response to this tour," Stuart tells Vertigo. "We've just been kind of overwhelmed with the reception of these tour dates."
"Originally it was one show in each city, but Sydney and Melbourne, the ticket sales were so amazing. The first show sold out about six weeks from the actual show so we thought we're just going to have to add more shows."
As well as playing three shows at Oxford Art Factory, Hermitude is performing at UTS' O' Week event, O' Fest. But according to Stuart, this is just the beginning of another year that will hopefully be as chaotic and successful as 2012.
Last year, indie radio station FBi named Hermitude their most played artist of 2012, following the release of critically acclaimed record, Hyperparadise. They even managed to land the 18th spot in Triple J's Hottest 100. Now unless you've been living under a soundproof rock for the past few months, you would know that electro artist, Flume, who was dubbed one of the breakout stars of 2012, remixed the title track.
"His version is awesome and it just took off and became a massive song," says Stuart.
Over the past year, Dubs and Elgusto have also received a range of nominations and awards. Hyperparadise won Best Independent Dance/Electronic album at the Australian Independent Records Association Awards (AIR) and the duo won Best Live Music Act at FBi Radio's Sydney Music, Arts & Culture Awards (SMAC). More recently, the duo has been shortlisted for the Australian Music Prize along with Flume, Tame Impala, The Presets and others.
Hermitude joined the local hip hop scene in 2000 with the launch of their vinyl-only EP, Imaginary Friends. The esoteric vibe of the record had the band putting their most interesting foot forward and is now a sought-after collector's item.
"We grew up in the Blue Mountains and when we started writing this type of music we basically locked ourselves away in a studio," Stuart explains. "It was in the bush and isolated and we were kind of just in a state of Hermitude."
Stuart and Dubber enjoy a broad range of musical styles and love traveling the world within their music. Early on in their career, the boys stretched their horizons, dabbling in Cuban beats and oriental undertones.
"When I was about fifteen, I went to Cuba with my father and we spent about a month in Havana studying percussion over there," Stuart tells Vertigo.
"'Nightfall's Messenger' came from a Japanese record and yeah, we just like to keep our ears open and we're into a lot of different styles and this just comes through in the music when you make it."
Over the years, Hermitude has graced the stages of music festivals like Groovin' the Moo, Woodford Festival, Homebake and Rhythm and Alps (NZ).
"One of our favourites was Woodford Festival up in Queensland. It's just such a great vibe up there. Also Groovin' the Moo Festival was so fun 'cause we got to go to all the regional places we don't normally get to go to… [and] they really look after you as well. Also there's a festival in Berlin called Fusion Festival that we played at last year, which was just insane. It was really cool. In Perth we played over New Year's Eve and New Year's Eve is my birthday so I got sung 'Happy Birthday' by about 5000 people."
In the studio, the pair work relentlessly on their material. Being multi-instrumentalists, they have the advantage of being able to share the music-making process.
"It's pretty equal. We generally write together all the time. We have a studio in Leichhardt and we just come in every day and we sit down and look at each other and go 'what do we want to write today?' and maybe I'll kick out a beat and Dubs will start twinklin' on some synthesisers and we'll just go from there. We do a lot of everything."
Along with creating their own innovative material, Hermitude have collaborated with the likes of electronic duo, The Presets, and hip hop MC, Urthboy.
"Doing The Presets remix was really fun. 'Ghost Track' is an awesome song in itself. Having someone's music in the palm of your hands is a privilege. It's kind of like being given a gem of a song and being able to sort of put your own tastes and little styles into it. That was a really fun experience doing that and we really like their stuff. So it was really cool for us to be able to take one of their songs and rearrange it."
"Working with Urthboy is always really great. We've worked with him for a long time and doing the Smokey's Haunt album with him was really fun. We went down to Melbourne and we all sacked up in Bounce's Studio and we just had a whole bunch of sessions where we just wrote heaps of ideas and then we took all those ideas and pushed them out over a number of months. We just love working with Tim [Urthboy]. It's really nice to work with a rapper and stuff. Hermitude's all instrumental so it was fun for us to work with a vocalist."
According to Stuart, having good influences is one thing, but creating a groove of your own is another.
"I think a really good thing to do is to try develop your own sound. So obviously you take influence from different artists and everything but one of the best steps you can take, I think, is to develop your own thing because in the end that's what is going to set you apart from everyone else and make you unique."
Hermitude played at O' Fest on February 22.


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Keeping up with friends is faster and easier than ever with the Facebook Lite app! Use Facebook Lite as a friend app to sign in and follow your social network. The Facebook Lite app is small, saving you space on your phone and using Facebook in 2G conditions. Many of the classic Facebook features are available on the app, such as sharing on a timeline, tasting photos, finding people, and changing your profile and groups. Specific features include:
Supported Android
{4.0 and UP}
Supported Android Version:-
Jelly Bean(4.1–4.3.1)- KitKat (4.4–4.4.4)- Lollipop (5.0–5.0.2) - Marshmallow (6.0 - 6.0.1) - Nougat (7.0 – 7.1.1) - Oreo (8.0-8.1) - Pie (9.0)


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This property is currently owned on a PART SHARE basis. Any interested parties can purchase further part shares up to full 100% ownership at which time the property will revert to FREEHOLD tenure.
Having composite entrance door, central heating radiator, fitted cloaks cupboard and door leading to:-4.14m x 2.64m max
Having a comprehensive range of fitted units to include a single drainer sink unit, base units with worktops over, matching wall units, plumbing for automatic washing machine, further appliance space, fitted oven and hob with stainless steel cooker hood over, ceramic tiling central heating radiator, wall unit housing the gas central heating boiler and leaded double glazed window overlooking front elevation.3.96m x 2.90m
Having double glazed leaded french doors leading to the lovely rear garden and two central heating radiators,3.96m x 3.66m
Having dual aspect double glazed leaded windows to both rear and side elevation, central heating radiator and a range of fitted wardrobes.4.01m x 3.68m
Having leaded double glazed window overlooking the front elevation, central heating radiator and large walk-in storage cupboard fitted with shelving and a central heating radiator.3.96m x 2.87m max
Having panelled bath, low level WC, wash hand basin, fully tiled shower cubicle, central heating radiator, ceramic tiling and obscured double glazed window overlooking rear elevation.2.84m x 2.36m
The bungalow benefits from a large blocked paved driveway to the side, with gated access to the rear garden.
The front p
athway leads to the mainenatnce door, with shrubs borders. The lovely private rear garden comprises patio area with shaped lawn, being fenced to all boundaries.


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« Back to Menu
Corporate Box #5
Feeds 5-10 people
1/2 Dozen top sellers bread pieces
1/2 Dozen Tamales: Pork & Chicken
Individually wrapped tamales filled with either pork or chicken (both will be included in your order).
Tamales are a handmade corn based Mexican Masa filled with pork or chicken cooked in a red or green family recipe salsa then wrapped in corn husks and pot steamed to perfection.
Please contact us for the option below at (832) 986-9022
- Company Personalized Cookies (Your company's logo / Your Customers logo).


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KFC Global Day of Service (GDS) is a one day pre-IKV (International Kids' Village) event where CFC Kids for Christ members together with their friends and families, are able to put their faith into action and make a positive impact in the world. This will be joined by different countries doing acts of service on September 4, 2010.
The GDS is a response to the challenge of the 13th IKV theme, "World Changers." Through the kids' service, we will be able to inspire many people to do something about changing the world and bringing it back to the plan of God. With the many issues and problems that the kids and their families are facing today, we desire to address these concerns by building a generation of Christ-like leaders that will bring Christ's transforming love and making them World Changers.
Our main message during the GDS is "Kids Can." This is derived from Philippians 4:13, "I can do all things through Christ who strengthens me."
We hope to express three main points:
• Kids Can Lead
• Kids Can Serve
• Kids Can Make a Difference.
It is our hope that CFC's vision of "Families in the Holy Spirit Renewing the Face of the Earth" will become a reality through the ministry's efforts. The mission-vision of CFC Kids for Christ of "Bringing Kids to the Lord and Building a Bright New World" is an alignment to the CFC's vision. The GDS will help us in pursuing the dreams of our community for the society and the rest of the world.
FOR MORE INFORMATION, EMAIL US AT [email protected] or view the following:
-GDS Memo 1


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EVENT DATE: Jul 16, 2016
DEADLINE TO GIVE: Aug 31, 2016
'One Mile in Style' The Power Walk for Dress for Success Southern Nevada® .
Beat the heat and walk with us at this fun, family fitness indoor event celebrating women becoming their best selves! There will be family entertainment, local vendors and lifestyle personality, Elle Swan, to kick off the walk, as well as a pre and post party hosted by Drybar!
We encourage individual walkers and teams to show off their summer style – from sneakers to stilettos, hoodies to haute couture – and be part of a health conscious, fashionista family!
The Power Walk for Dress for Success is dedicated to empowering women on their quest for economic independence, through an active and healthy lifestyle. It serves as a "walking testament" to the need for success-driven women to become healthy in all aspects of their lives. Studies prove that employees who exercise for at least 30 minutes, three times a week, are 15% more likely to have higher job performance, and 25% more likely when eating healthy.
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October 16th, 2018 by WCBC Radio
CSX Corporation (NASDAQ: CSX) today announced third quarter 2018 net earnings of $894 million, or $1.05 per share, versus $459 million, or $0.51 per share in the same period last year (a 106 percent increase). CSX's operating ratio set a company third quarter record of 58.7 compared with 68.4 percent in the prior year (a 970 basis point improvement).
"This quarter highlights the progress towards our transformation as we aim to deliver industry leading service to our customers," said James M. Foote, president and chief executive officer. "I am proud of our team of hard-working employees who were able to produce these results."
Revenue for the third quarter increased 14 percent over the prior year to $3.13 billion, supported by broad-based volume growth, increases in fuel recovery, favorable mix, higher supplemental revenue and pricing gains. Expenses declined 2 percent year over year to $1.84 billion, as expenses associated with increased volume and higher fuel prices were more than offset by efficiency gains as CSX continues to implement its scheduled railroading business model. This combination yielded operating income growth of 49 percent for the quarter to $1.29 billion compared to $868 million in the same period last year.
CSX executives will conduct a conference call with the investment community this afternoon, October 16, at 4:30 p.m. Eastern Time. Investors, media and the public may listen to the conference call by dialing 1-888-327-6279 (1-888-EARN-CSX). For callers outside the U.S., dial 1-773-756-0199. Participants should dial in 10 minutes prior to the call and enter in 3276279 as the passcode.
In conjunction with the call, a live webcast will be accessible and presentation materials will be posted on the company's website at http://investors.csx.com. Following the earnings call, an internet replay of the presentation will be archived on the company website.
This earnings announcement, as well as additional detailed financial information, is contained in the CSX Quarterly Financial Report available through the company's website at http://investors.csx.com and on Form 8-K with the Securities and Exchange Commission.


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Today has been, so far, maybe the best day of this year. Maybe I'm overcompensating because I was so recently broken up with and, of course, I had plenty of great times with him this year before that happened. But I have always felt that the happiness experienced in solitude has a magic to it … Continue reading ✨ The Magic Of Solitude ✨
Category: self love
Thoughts On… A Week Of Self Love
I knew when I was starting out with this blog that I'd fall into a slump at some point. I knew it the most when the writing was flowing out of my pen and everything was new. I thought: it won't always be like this. For a creative process to be perpetually smooth sailing - … Continue reading Thoughts On… A Week Of Self Love
Self Love – Setting Intentions
In the run-up to this post, I came across a well-timed Facebook post from Blurt Foundation - a charitable organisation dedicated to helping people with depression. The post was about self love and it was accompanied by a lovely diagram (see below). The self love "hedgehog" displays 7 conclusive methods to treat ourselves with more compassion and kindness. … Continue reading Self Love – Setting Intentions


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Persistent high-risk HPV infection is considered as a major cause of cervical cancer. Nevertheless, only some infected individuals actually develop cervical cancer. The RIG-I pathway in innate immunity plays an important role in antivirus response. Here, we hypothesized that altered function of mitochondrial antiviral signaling protein (MAVS) and mitochondrial TNF receptor–associated factor 3(TRAF3), key molecules downstream of the viral sensors RIG-I, may impair their ability of clearing HPV and thereby influence the risk for cervical precancerous lesions. To investigate the effects of MAVS and TRAF3 polymorphisms on susceptibility to cervical precancerous lesions, 8 SNPs were analyzed in 164 cervical precancerous lesion cases and 428 controls. Gene–environment interactions were also calculated. We found that CA genotype of rs6052130 in MAVS gene were at 1.48 times higher risk of developing cervical precancerous lesion than individuals with CC genotype (CA vs. CC: ORadjusted = 1.48, 95% CI, 1.02–2.16). In addition, a significant synergetic interaction between high-risk HPV infection and rs6052130 was found on an additive scale. A significantly decreased risk of cervical precancerous lesions for the TC genotype of rs12435483 in the TRAF3 gene (ORadjusted = 0.67, 95% CI, 0.45–0.98) was also found. Moreover, MDR analysis identified a significant three-locus interaction model, involving high-risk HPV infection, TRAF3 rs12435483 and number of full-term pregnancies. Our results indicate that the MAVS rs6052130 and TRAF3 rs12435483 confer genetic susceptibility to cervical precancerous lesions. Moreover, MAVS rs6052130–mutant individuals have an increased vulnerability to high-risk HPV-induced cervical precancerous lesions.
With about 530,000 new cases annually, cervical cancer is a major malignant disease among women and accounts for 8% of the total cancer-related mortality (1, 2). Virtually all cervical cancers result from a persistent infection with certain high-risk types of the human papillomavirus (hrHPV) family (3). Cervical cancer develops through a multistep process, with three cervical intraepithelial neoplasia grades, 1 to 3 (CIN1–3; ref. 4). The transition from CIN to invasive cancer will take several years, even decades, which offers us many opportunities for intervention and only a minority of the infected individuals go on to develop invasive cervical cancer (5). Available data showed that an approximate 30% to 60% of sexually active women infected with genital HPV yet do not develop cancer, and the large majority of infections are cleared by the host immune system (2, 6). Therefore, HPV itself is not sufficient to cause cervical cancer and a variety of environmental and host factors are involved in the development of cervical cancer (7, 8).
Type I IFNs are critical components of the innate defense against viral infections (9), which can interfere with the viral transcription of HPV16 and HPV18 (10). However, the pathways by which HPV DNA virus triggers innate defenses are not well-established (11). Detection of viral nucleic acids within the cytoplasm via either the RIG-I–MAVS signaling axis or the cGAS–STING DNA–sensing pathway leads to the production of antiviral IFNs (12, 13), in which retinoic acid-inducible gene I (RIG-I) and mitochondrial antiviral signaling protein (MAVS) mediate IFN production in response to cytosolic double-stranded RNA or single-stranded RNA containing 5′-triphosphate (5′-ppp; ref. 14). Recently, there are indications that RNA-sensing pathways may modulate host responses against DNA infections (15). Chiu and colleagues (14) demonstrated RNA polymerase III (RNA Pol III) detects cytosolic DNA and converts cytosolic AT–rich DNA into RNA, then is sensed by RIG-I, and recruits the adaptor protein MAVS (16). MAVS further interacts with TNF receptor–associated factor 3 (TRAF3) to recruit downstream IRF3 and NFκB-activating kinases, resulting in IFNβ immune response (17), which can inhibit replication of HPV (18).
MAVS, also known as IPS-1, VISA, and Cardif (19, 20), plays a central role in regulating the complex events that lead to either antiviral or inflammatory responses (17). Melchjorsen and colleagues (21) found that the early IFN response was highly dependent on MAVS, because knockdown of MAVS in the macrophages resulted in a strong reduction in the levels of IFNI. Although, how MAVS functions or is regulated remains enigmatic. A recent study reported that the C-terminal TIM region of MAVS (455-PEENEY-460) was shown to bind TRAF3 and exclusively mediate the induction of IFN expression (22). Interestingly, all of the major viral recognition pathways characterized so far, require TRAF3 to initiate IFN production (23). Oganesyan and colleagues demonstrate that TRAF3 is a major regulator of type I IFN production and the innate antiviral response. Furthermore, gene deletion studies have identified TRAF3 as a critical mediator involved in the induction of the IFNs by the RIG-I pathway (24). Nevertheless, the true function of TRAF3 remained a mystery.
Genetic variability of the host has great impact on the outcome of an HPV infection, especially genetic factors that control the innate immunity (25). Moreover, the innate immune system plays an important role in cervical cancer progression (26). Thus, we hypothesized that individuals' genetic differences in the MAVS and TRAF3 gene could influence the expression of IFNI and the host antiviral response, and thus affect the individuals' susceptibility of developing cervical cancer.
In this study, eight polymorphisms in two candidate genes, including the MAVS gene (rs3746660, rs6052130, rs6116065, rs8116776, and rs914294), the TRAF3 gene (rs7156191, rs8022180, and rs12435483) were genotyped, and the possible associations of these SNPs with cervical precancerous lesions were investigated. Furthermore, we sought to study the potential interactions between these SNPs and environmental factors in the etiology of cervical precancerous lesions in the Chinese population.
Materials and Methods
In this study, all of the cervical specimens were collected with a broom-like device (Qiagen) and placed into a ThinPrep Pap test vial containing PreservCyt Solution. Referral Pap specimens were processed locally using the ThinPrep 2000 System (Hologic) and evaluated for routine screening cytology (8). A Pap smear was positive for squamous intraepithelial lesion (SIL) if low- (LSIL) and high-grade SIL (HSIL), as classified according to the Bethesda Classification System, was detected (27). This study included 164 SIL patients, 120 cases with LSIL (73.2%), and 44 cases with HSIL (26.8%). Four hundred and twenty eight control subjects were randomly selected from those without intraepithelial lesion or malignancy during the same period from the area of residence of the cases. All participants resided in southern China and were unrelated ethic Han Chinese. Participant written consent and ethical approval from the Ethics Committee of Medicine of Jinan University (Guangzhou, Guangdong, China) were obtained.
HPV testing
According to the manufacturer's instructions, total DNA from cervical cells was extracted using a commercial magnetic beads kit (Chemagen; PerkinElmer). Then, the MassARRAY (Sequenom) technique based on matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to detect 16 HPV types including 2 low-risk (6, 11) and 14 high-risk (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68; ref. 28). We performed all of the procedures in the clinical standard laboratory of Beijing Genomics Institute (Shenzhen, China).
Genotype analysis
Genomic DNA from peripheral blood was extracted with phenol–chloroform DNA extraction methods. The samples were stored at −20°C until they were used. The DNA concentration was determined using a spectrophotometer (NanoDrop ND-1000, PerkinElmer). Samples with a mean OD260 nm/OD280 nm of 1.8 to 2.0 and DNA concentration > 20 ng/μL were considered to be free of contamination.
Tag SNPs (tagSNP) across the MAVS and TRAF3 genes were determined through a search of the HapMap database. Furthermore, the tagSNPs were selected using the following criteria: (i). the population of the HapMap selected Chinese Han Beijing (CHB); (ii). using Haploview by Tagger function; (iii). SNPs with a minor allele frequency (MAF) greater than 5%; (iv). the pairwise tagging with r2 ≥ 0.8 (29, 30). Eventually, 8 tagSNPs were selected, including rs3746660, rs6052130, rs6116065, rs8116776, rs914294 in MAVS and rs7156191, rs8022180, rs12435483 in TRAF3. Supplementary Table S3 shows these SNP sites and locations.
MALDI-TOF MS method was used to genotype these 8 SNPs. Detailed primer and amplified length for the determination of the 8 SNP genotypes is provided in Supplementary Table S2. Sequenom MassARRAY RS1000 was used for genotyping and the related data were performed using Typer software version 4.0. The method of this part was described in our previous study (8).
qRT-PCR analysis
With Ficoll–Hypaque density-gradient centrifugation, the peripheral blood mononuclear cells (PBMC) were isolated from EDTA-anticoagulated blood. TRIzol (Invitrogen) was used to extract total RNA from PBMCs. Then, we used a transcriptase cDNA kit (Takara-PrimeScript RT Master Mix Kit) to finish reverse transcription. And qRT-PCR analysis was further performed to quantify the mRNA expression of MAVS and TRAF3 genes with the SYBR PrimerScript RT-PCR Kit (Takara). On a Bio-Rad's CFX96 RT System (Bio-Rad Laboratories), the assays were performed. Cycle conditions were 95°C for 30 seconds followed by 45 cycles at 95°C for 5 seconds and 60°C for 30 seconds. The expression of individual MAVS and TRAF3 measurements was calculated relative to expression of β-actin using 2−ΔΔCt method (8). Mann–Whitney test was used to test the differences of relative mRNA levels between the cases and controls.
Statistical analysis
The dependence of the allele frequencies between the cases and controls was calculated by the χ2 test. Moreover, we used a t test or χ2 to evaluate differences in the demographic characteristics and high-risk HPV infection status between the cases and controls. Logistic regression was used to calculate ORs and their relative 95% confidence intervals (CI) for risk estimation. The haplotypes of MAVS and TRAF3 gene were analyzed using SNPStats online program (https://www.snpstats.net). Haplotypes with a frequency less than 0.03 would be excluded from analysis. All MAVS and TRAF3 gene polymorphisms identified in the best models of gene–environment interactions were calculated using MDR software (version 1.0.0) and MDR permutation testing software (version 1.0 beta 2; refs. 30, 31). The best model for each order of interaction was selected by cross-validation consistency (CVC) and testing-balanced accuracy (TBA). Interaction models showing highest TBA and CVC were further tested by 1,000 folds permutation tests and a sign test P value of 0.05 (32). All other statistical analyses were performed using SPSS software v.16.0 (SPSS, Inc.).
The interaction effects were determined on the multiplicative and additive scale. Interaction on the multiplicative scale was calculated by logistic regression model. On the additive scale, three measures to examine biological interaction were as follows: (i) attributable proportion due to interaction (AP), (ii) relative excess risk due to interaction (RERI), and (iii) synergy index (S; ref. 33). RERI > 0, AP > 0, or S > 1 indicates biological interaction (34, 35). Furthermore, S > 1 for synergetic effects and S < 1 for antagonistic effects (36, 37).
Among cases and controls, a binary classification was used both for high-risk HPV infection (high-risk infection vs. low-risk HPV infection or non-HPV infection) and for genotypes (homozygous for the major allele vs. one or two copies of the minor allele). The risk for cervical precancerous lesions for a given SNP and high-risk HPV infection status was expressed by ORi,j where the first index (i) indicated the high-risk HPV infection status coded as 0 for low-risk HPV-infected or non-HPV–infected and 1 for high-risk HPV-infected subjects, and the second index (j) indicated the SNP genotype, coded as 0 for subjects homozygous for the major allele and 1 for subjects bearing one or two copies of the minor allele. Subjects who were low-risk HPV-infected and homozygous for the major allele were considered the reference group, thus coding their cervical precancerous lesions risk as OR00=1. The relative ORs were obtained by logistic regression. The CIs were calculated by the regression coefficients and the corresponding covariance matrix (34). Excel spreadsheet (www.epinet.se) was used to calculate additive interaction: S, RERI, and AP and their corresponding CIs (34).
Hardy–Weinberg equilibrium was reassessed for the 8 SNPs comparing the observed genotype frequencies with the expected frequencies using χ2 test with significance level at P < 0.05 (38). The Akaike Information Criterion (AIC) was calculated for each genetic model and the genetic model with the smallest AIC was selected (39).
Statistical significance was set at P < 0.05 for each analysis.
The association of SNPs in MAVS and TRAF3 genes with cervical precancerous lesions
The demographic characteristics are shown in Supplementary Table S1. All observed genotype frequencies in both SIL cases and controls conform to Hardy–Weinberg equilibrium (P > 0.05). As shown in Table 1, no SNP was found to be associated with the risk of cervical precancerous lesions under the allelic model.
The genotype association of 8 SNPs in the MAVS and TRAF3 genes with cervical precancerous lesion risk was shown in Table 2, in which rs6052130 in MAVS gene and rs12435483 in TRAF3 gene were significantly associated with cervical precancerous lesion. After adjusting for age, passive smoking, BMI and initial pregnancy of age, individuals who carried CA and CA+AA genotype in MAVS rs6052130 were at 1.48 and 1.47 times higher risk of developing cervical precancerous lesion than individuals with CC genotype (CA vs. CC: ORadjusted = 1.48, 95% CI, 1.02–2.16, P = 0.041; CA+AA vs. CC: ORadjusted = 1.47, 95% CI, 1.02–2.12, P = 0.038). In addition, TC and TC+TT genotypes in TRAF3 rs12435483 were found to have a significant protective effect (ORadjusted = 0.67, 95% CI, 0.45–0.98, P = 0.041 and ORadjusted = 0.69, 95% CI, 0.48–0.99, P = 0.044, respectively) compared with CC genotype. Moreover, the AIC values in the dominant model were the smallest, 698.1 and 698.3 for rs6052130 and rs12465483, respectively (Supplementary Fig. S1) while the other 6 SNPs were not observed to be relevant to the risk of cervical precancerous lesions.
Haplotype analysis
No haplotype in MAVS and TRAF3 genes was found to be associated with cervical precancerous lesions (Supplementary Table S4).
Additive and multiplicative interaction
The interactive effects of high-risk HPV infection and each SNP were calculated on the basis of an additive scale and multiplicative scale. On the additive scale, we examined the RERI, AP, and S between MAVS rs6052130 (wild or mutant) and high-risk HPV infection (positive or negative) in their association with cervical precancerous lesion. The RERIadjusted, APadjusted, and Sadjusted were 3.10 (95% CI, 0.02–6.18), 0.42 (95% CI, 0.13–0.71), and 1.93 (95% CI, 1.03–3.61), respectively, suggesting that there was statistically significant synergistic interaction (Table 3). And the OR of MAVS rs6052130 mutant and high-risk HPV infection on cervical precancerous lesions was the highest (ORadjusted, 7.46; 95% CI, 4.19–13.26; Fig. 3). The multiplicative interaction analysis revealed no interaction between high-risk HPV infection and each SNP (Table 3).
The results of MDR analysis for one- to four-factor models were shown (Table 4). The best interaction model of predicting cervical precancerous lesions risk was the three-factor model, including high-risk HPV infection, TRAF3 rs12435483, and number of full-term pregnancies, which yield the highest TBA (69.67%) and the maximum CVC (10/10) and P for permutation test = 0.0000–0.0010.
Three-way interaction between high-risk HPV infection, TRAF3 rs12435483, and number of full-term pregnancies on the risk of cervical precancerous lesions
We further performed a risk group analysis of the combinations of three factors, hrHPV infection, mutant-type of TRAF3 rs12435483, and higher number of full-term pregnancies (Fig. 4). Individuals with negative high-risk HPV infection, wild-type of TRAF3 rs12435483, and term births ≤2 times were used as reference group. Independent of the status of rs12435483, those with high-risk HPV exposure and low number of full-term pregnancies was decreased about 2 times risk of cervical precancerous lesions compared with those with more than 2 times full-term pregnancies.
Functional studies
To explore the effects of different genotypes on gene expression, the MAVS and TRAF3 mRNA expression levels in healthy individuals were detected and no significant difference was observed between the CA+AA genotype and CC homozygotes in MAVS mRNA expression (P = 0.438; Fig. 1A). The TRAF3 mRNA level in TC/TT individuals of SNP rs12435483 was significantly higher than in CC individuals (P = 0.028; Fig. 2A).
To further study the functional role of MAVS/rs6052130 and TRAF3/rs12435483 in the expression of IFN, we carried out a RT-PCR assay to evaluate the mRNA levels of IFNβ expression from healthy individuals.
As shown in Fig.1B, the IFNβ mRNA level in CA/AA individuals of SNP MAVS/rs6052130 was significantly lower compared with CC individuals (P = 0.048).
We also investigated whether the expression of IFNβ was affected by different TRAF3/rs12435483 genotypes. The IFNβ mRNA level in CT/TT individuals of SNP rs12435483 was significantly higher than in CC individuals (P = 0.039; Fig. 2B).
Cervical precancerous lesions is a complex disease with a multifactorial etiology (40). The role of RIG-I pathway in host defense to multiple RNA viruses is well established; however, no association among the population about polymorphisms in the RIG-I pathway involved in the host defense to HPV DNA viruses in cervical precancerous lesions has been reported. And the roles of the key gene in RIG-I pathway in the pathogenesis of cervical precancerous lesions are still not well known. Here, we found that MAVS and TRAF3 genes influence the risk of cervical precancerous lesions, which might provide new insights into the modulation of innate immune response against HPV.
A recent study indicated that the RIG-I–MAVS signaling pathway plays an important role in the innate immune response to HPV infection (41). rs6052130 resides in the intron 5–6 region of the MAVS gene. We found that the heterozygous genotype (CA) in MAVS rs6052130 was significantly associated with an increased risk of cervical precancerous lesions. Previous studies have confirmed that SNPs in MAVS exerted inhibitory effects on antiviral signaling in response to dsRNA (42). In addition, Lad and colleagues (43) found the splicing variants of MAVS, MAVS 1a, inhibits RIG-I and MAVS activity. Interestingly, our study also observed a synergetic interaction between high-risk HPV infection and MAVS rs6052130, which indicates that the rs6052130 variant in the MAVS may inhibit antiviral signaling in response to HPV. Our functional analysis in PBMCs showed that the MAVS rs6052130 CA or AA genotype might downregulate the production of IFNβ, although a significant difference of MAVS mRNA expression in different rs6052130 genotypes was not observed. Therefore, we speculated that the rs6052130 variant in the MAVS gene might contribute to dislocation of MAVS from the outer mitochondria membrane (42), which may lead to loss of RLR antiviral signaling in response to HPV, thereby promoting evasion of HPV, leading to a more robust infection and ultimately contributing to an increased risk of cervical precancerous lesions. More studies are warranted to elucidate these speculations.
Furthermore, MAVS was shown to interact with TRAF3 through TRAF-binding consensus sequences (13). TRAF3 bridged the upstream MAVS and downstream kinase TBK1 and assembles the active MAVS–TRAF3–TBK1 signaling complex (44). The expression of type I IFN elicited by the infection of RNA viruses was severely reduced in TRAF3-deficient mouse embryonic fibroblast cells, indicating a crucial role for TRAF3 in RIG-I signaling. Although, the molecular mechanisms by which TRAF3 is involved in DNA viral response pathways remain unclear. rs12435483 is located in intron 2–3 region of TRAF3 gene. This polymorphism was suggested to increase susceptibility to type II diabetes (45). In our study, the TC heterozygote in rs12435483 was observed to decrease the risk of cervical precancerous lesions by a 0.67-fold. Moreover, we found that the minor allele of rs12435483 was associated with an increased TRAF3 expression phenotype at mRNA levels, and a significant upregulation of IFNβ mRNA expression by PBMCs in TC or TT genotype of rs12435483 compared with CC carriers was also observed. As our previous study showed, an intronic polymorphism also played an important role in mRNA or protein expression (8). We, therefore, propose that carriers of the rs12435483 variant might enhance the TRAF3 transcriptional activity, resulting in a higher level of TRAF3 expression and thereby enhancing IFNβ induction, then contributing to clearance of the HPV virus (8, 46) and ultimately contributing to a decreased risk of cervical precancerous lesions. Modulating TRAF3 activity may be a productive approach to host-directed cervical precancerous lesions therapy. These findings need to be further confirmed in a larger population. We have also found an interaction between high-risk HPV infection and TRAF3 rs12435483, which was especially strong among women with more than 2 times full-term pregnancies (Fig. 4). It is therefore likely that a better understanding of the interplay between different risk factors may shed light on the pathogenesis of cervical precancerous lesions (47).
This study has important clinical implications. Because of hereditary nature of the rs6052130 genotype, effective intervention and prevention measures can be implemented by monitoring high-risk HPV infection status. Moreover, the interactions between rs6052130-mutant and high-risk HPV exposure are important at the population level because among rs6052130-positive women, once changing infection status from high-risk HPV to low-risk HPV would reduce the incidence rate of cervical precancerous lesions by about 40.9% (Fig. 3). Consequently, we suggest that high-risk HPV infection should be prevented in healthy individuals, especially in those women with rs6052130 variant. It is a priority for healthy rs6052130-mutant individuals receiving the HPV vaccine and regular screening to measure status of high-risk HPV infection. Moreover, our data provide novel insight into how MAVS and TRAF3 functions in antiviral innate immune signaling. A further investigation of the RIG-I signaling pathway and a better understanding of how innate immune system works would be a novel target for anti-HPV strategies in the prevention and treatment of cervical precancerous lesions (48).
Several limitations of our study merit further discussion. First, the limited sample size reduced the study power to detect the association of SNP with cervical precancerous lesions. Thus, larger studies and an independent cohort should be taken to validate the association between MAVS rs6052130, TRAF3 rs12435483 polymorphisms, and cervical precancerous lesions. And we would like to mention that we did not include the lesion stratification results because Pap smear might not be reliable enough in grading the severity of SIL (49). Therefore, SIL was only divided as present or absent. Although the methods of calculating interactions based on the MDR could apply to multiple factors at multiple levels, the mechanism how the three factors, including high-risk HPV infection, TRAF3 rs12435483, and number of full-term pregnancies, contribute to cervical precancerous lesions is not well understood. Taking into account that no report has been studied that the expression level of MAVS/TRAF3 in cervical cells, it remains to be elucidated whether an increase of MAVS/TRAF3 expression in cervix correlates with an increase of their expression in PBMCs. It should be noted that the mRNA levels were only tested in healthy control individuals, because the expression of various genes related to the inflammatory response may change during the clinical course of cervical precancerous lesions (50).
In conclusion, our study provides evidence that the MAVS rs6052130 and TRAF3 rs12435483 polymorphism influence the risk of cervical precancerous lesions. In addition, this study also observes that two-way and three-way gene–environment interactions exist in the etiology of cervical precancerous lesions. Our data support the mechanism research that RIG-I signaling pathway plays a crucial role in the antiviral host response to HPV in Chinese. This study provides new insights into the mechanism of HPV infection and pathogenesis, and suggests that MAVS and TRAF3 are new potential antiviral targets in the prevention and treatment of HPV-associated cervical precancerous lesions.
Disclosure of Potential Conflicts of Interest
No potential conflicts of interest were disclosed.
Authors' Contributions
Conception and design: G. Yang, C. Jing
Development of methodology: D. Liu, Y. Han, J. Wu, S. Huang
Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc.): D. Liu, Z. Wen, X. Huang, X. Ye, C. Huang
Analysis and interpretation of data (e.g., statistical analysis, biostatistics, computational analysis): D.Xiao, C. Zeng, Z. Zhou, C. Guo, G. Yang, C. Jing
Writing, review, and/or revision of the manuscript: D.Xiao, C. Jing
Administrative, technical, or material support (i.e., reporting or organizing data, constructing databases): D.Xiao, Y. Wang, M. Ou, G. Yang, C. Jing
Study supervision: X. Wei, G. Yang, C. Jing
This work was supported in part by the Major Research Plan of the National Natural Science Foundation of China (grant no. 91543132); National Natural Science Foundation of China (grant no. 81541070, 30901249, and 81101267); the Guangdong Natural Science Foundation (grant no. 2018A030313601, 10151063201000036, S2011010002526, and 2016A030313089); Guangdong Province Medical Research Foundation (grant no. A2014374, A2015310) and Project from Jinan University (grant no. 21612426, 21615426, JNUPHPM2016001, and JNUPHPM2016002).
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Note: Supplementary data for this article are available at Cancer Prevention Research Online (http://cancerprevres.aacrjournals.org/).
- Received May 11, 2018.
- Revision received August 28, 2018.
- Accepted November 5, 2018.
- ©2018 American Association for Cancer Research.


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