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20 Questions with Katie Shibale, Account Manager at MoxiWorks
Katie Shibale is on the front lines of MoxiWorks, always working hard to delight clients, making sure they are well taken care of from all angles: training, materials, integration… the list goes on! Being an Account Manager is a tough job, and we're thankful Katie and her awesome teammates are here to do it.
We thought it'd be fun to sit down with Katie and get to know her just a little bit better. If you're reading this and happen to be one of our clients, there's a good chance you've experienced Katie's brilliant work ethic, communication, and determination to deliver the best to your brokerage.
Without further ado, here's 20 questions with Katie:
1. How long have you been at MoxiWorks?
1 year this month!
2. Best and worst thing about living in the Seattle area?
The traffic, of course, is the worst.
The views are by far the best. Between the mountains, the water, and the trees, it is breathtaking here.
3. What has been your favorite achievement so far at MoxiWorks?
There are two in particular.
The first, is creating and implementing a training process for incoming Account Managers. I took experiences from my career and was able to create a manual that made the transition to being an Account Manager at MoxiWorks more seamless. In doing so, I was able to watch a fellow Account Manager go through that and become a kickass AM quickly.
The second, is completing my first official onboarding with a well-known brokerage in the industry. I created a successful launch and relationship with them, and that's a great feeling.
4. What's your philosophy in life?
Do what you love, love what you do.
5. What would you eat for your last meal?
Really good authentic Mexican food (and some whiskey).
6. What's your favorite thing about MoxiWorks?
The culture, honestly. Looking at how we all work together… and I love the fact that we are a name versus a number (like you tend to be in big companies). Each department actually works together versus everyone just working on their own path.
7. What's the best part about working with your clients?
There are so many, so I'll try to keep it short. First, building strong, trusting relationships that allow us to truly partner with one another. I love when they have that "ah-ha" moment once they truly find the value in what we do here. That's the moment they begin truly promoting the products, helping us (MoxiWorks) further innovate, and agents become more productive. Finally, when I get to meet the agents in person. Watching them get excited about the different tools and how easy they are to use is rewarding.
8. NFL or MLS?
That's a mean question, but NFL had my heart first!
9. Do you consider yourself an introvert or an extrovert?
Introvert – or maybe the one in-between (ambivert). I think I come off as an introvert, but when you get to know me you can't shut me up.
10. What keeps you at MoxiWorks?
My team for sure. I love the collaboration between us. I know this may sound corny, but we're like a family. We're constantly joking around, supporting one another, and really challenging each other to become better (all around people) each day.
11. Cats or dogs?
Definitely, dogs!
12. What's your favorite part of the day?
The evening because I get to spend time with my son.
13. What was your first job?
I was a Nike sales associate.
14. What interested you about MoxiWorks in the first place?
I was originally in the mobile apps industry, so getting into the software industry sounded interesting. After I talked to Kylah (Director of Integrations and Partnerships at Moxi), I realized the culture was exactly what I was looking for. Ultimately, I knew this was going to really stretch me, challenge me, and allow me to grow in all areas.
15. What do you do when you're not working?
I'm on the sidelines at my son's football, soccer, or basketball games cheering him on.
16. Did you choose this career path, or did it choose you?
It definitely chose me. I started in the education field, that's what my degree is in. I had a friend who worked at a mobile app company and had an opportunity to work for them too, but as a remote employee. It worked great since I could work from home as a mom. From there, I got into software. I never expected to be here, but grateful that others saw my potential and took a chance on me.
17. What does your ideal Saturday look like?
Start out with a cup of coffee for sure, and then either watch my son play sports (or play sports myself). And if my son is at his dad's, then hanging out with girlfriends with a nice bottle of wine and a good movie.
18. How would you describe your communication style?
I'm kind of an over communicator and can be assertive, especially if I'm confident in what I am saying. I prefer going up to someone and verbally talking things through, as it is really important in understanding exactly what's happening to help avoid any confusion.
19. What stands out to you about real estate technology since you've joined the industry?
Since I'm newer to the real estate industry, I'd say understanding how technology has increased at such a rapid pace. It's also fascinating how a lot of real estate agents embrace it, but there are way more who wish it never came to real estate. Being able to create something really simple and professional is essential to technology adoption.
20. What is your biggest challenge right now?
I would say that each brokerage/each client that I work with has different needs, especially based on the market they are in. Trying to create a product or tool that really satisfies each of their needs is challenging and then trying to find a workaround that they're pleased with has its own challenges as well.
Thanks, Katie for letting us ask you these questions! But more importantly, thank you for contributing to Moxi and our amazing culture! #ItTakesMoxi


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Will's Training Table to temporarily close for remodel
The new Sanford-owned, sports-themed restaurant at the Sanford Sports Complex includes bar favorites, healthier options and a range of drinks including a bubblegum martini.
Will's Training Table is temporarily closing in order to accommodate a major remodel.
The closure of the bar and restaurant adjacent to the Sanford Sports complex in northwest Sioux Falls will begin on Aug. 20, and will be effective for four to six weeks, according to sanfordpentagon.com.
As the project progresses, an exact reopening date will be determined and announced. The entire expansion is expected to be complete in February 2019.
The upgrades include an expanded kitchen, two-story addition to the northwest corner and walkout patios on both the upper and lower levels.
The expansion will nearly double the restaurant's size, growing the restaurant's seating capacity from 260 to 450.
The east-side patio will be dismantled and re-engineered into an enclosed event space with a garage door, an area dubbed the Tailgate Area.
Sanford Health took ownership of the building at 4021 N. Bobhalla Drive last year and transformed it into a sports-themed restaurant and bar.
To learn more, check out our previous coverage: Northwest Sioux Falls restaurant adding tailgate zone, fine dining, second-floor patios

subset: commoncrawl


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|Post Date
|Feb 21, 2015
|Expiry Date
|May 23, 2030
|Send Email Message
|West End Road, Negril,, Westmoreland, Jamaica View Map
|Office Phone
|(876) 957-4395
The Samsara Resort offers five room types: A Garden View Cottage, an Ocean View Cottage, Ocean View Hotel Room, King Cottage and the Pillar Houses.
Samsara Rates
At Samsara Cliffs Resort (and at Legends Beach Resort as well) you can book either an all-inclusive Negril vacation or a room-only package. The choice is yours!
Rates vary by season and by number of people in the room. We do this to give you the best price we possibly can, but if you find it confusing, please just contact us and we'll be happy to explain.
The All-Inclusive Vacation Package offers great value! It's an ideal way to guarantee an economical, worry-free holiday. This is a flexible option which can be booked for the entire stay or just a few days. At Samsara and Legends, we strive to create a comfortable home-away-from-home atmosphere that keeps visitors coming back to our Negril Resorts year after year.
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This morning I called the store in Berwick and they have the stainless steel bolts I am looking for, so I planned to go to Berwick today.
I told Sheila about my tentative departure date, she laughed and said, Assuming everything goes according to plan! Which it hasn't so far, why would now be any different?
After walking the dogs and cleaning up after breakfast and loading the truck, I left for Berwick just after noon. A very pleasant drive along Highway 221, "The Back Road." And I get to Berwick around 1.00pm to discover that the store is closed, they close at noon on Saturdays!
Now of course that means I won't be able to get the bolts until Monday.
Sheila may be right.
I tried to think of other things I might accomplish on this trip, I stopped in at the Berwick Frenchy's (chain of secondhand clothing shops here) but didn't see anything that appealed. I debated trying the Canadian Tire store on the off chance they had what I wanted, but couldn't face a Saturday afternoon there.
I ended up driving back to Canning and stopping at the building supply store there to enquire about urethane. I bought a litre of spar varnish and some Varsol. The lady there recommended some strips of pipe insulation to use on my tie-downs to keep them from rubbing on the kayak when I put it on the roof rack, so I bought a couple of them too.
Then I stopped at the butcher shop for a bone for Dobby. It's a real old fashioned butcher shop, I half expected to see sawdust on the floor. All the cuts of meat are wrapped in paper, and there was a pile of dogbones on the corner of the big chopping block.
I've thought of a couple of things I could do before Monday, I might be able to start putting urethane on the hull. I think I have to install the foot pedals before the urethane, and they already have stainless steel bolts.
I have stainless steel bolts for the back rest too, but I am not sure whether the washers for them are stainless steel, I may have to wait on that. But I can drill the holes for that and for all the deck rigging.
I think I can cut out all the strapping too, and put the buckles on the straps. Then on Monday I'll go back to Berwick for the bolts, and once they are installed I can urethane the deck.
In theory, that means I'll be done!
When we got home around 2.30pm, I gave Dobby his bone. He loves it!
He's still pretty scrawny, all my efforts to fatten him up are in vain. But, I got an idea to feed him root vegetables, I recall the pet food store lady telling me that potatoes are hypoallergenic for dogs, and that really it is protein that a dog is most likely to react to. So maybe if I fill him up on potatoes and carrots and beets...
I made a root vegetable stew for Dobby this afternoon, he loves it.
In the late afternoon I started drilling holes in the kayak, a half inch hole for the bowline at the bow of the boat, and quarter inch holes in the back rest and thigh braces to attach the back rest to the kayak.
In the evening I cut up the strapping into twelve pieces and attached the buckles to the pieces for six straps, three for each hatch. I used a cigarette lighter to melt the ends of the straps to prevent them from unravelling.
Scoring Practice
6 hours ago


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Statistics Overview
A Numbers Count statistics diagram
Listed in Teaching Materials | resource by group Numb3r5 Count!
Version 1.0 - published on 09 Sep 2019 doi:10.25334/XTE7-QC92 - cite this
Licensed under CC Attribution-ShareAlike 4.0 International according to these terms
This diagram is an overview of the relationships between statistical concepts and approaches.
This resource is a part of the Numbers Count Project.
- statistics overview.jpg(JPG | 1 MB)
- License terms
Cite this work
Researchers should cite this work as follows:
- Neuhauser, C. (2019). Statistics Overview. Numb3r5 Count!, QUBES Educational Resources. doi:10.25334/XTE7-QC92
Numb3r5 Count!
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Crop Insurance Implications of Planting Crops Early
Paul D. Mitchell, Agricultural and Applied Economics, UW-Madison
With the continuing record-breaking temperatures and sunshine warming soils this year, some farmers may be thinking of planting their crops earlier than usual. Climatologists and meteorologists, and many other people, will remind us all that we could still get severe frosts. Regardless of these warnings, some farmers have and/or may plant early. This short piece is just to remind famers of the crop insurance implications if they should choose to plant early.
Most Wisconsin farmers buy crop insurance for their major crops, such as corn and soybeans, with a sales closing date of March 15th. Among its many details, the crop insurance contract specifies an "earliest planting date". For corn, the official RMA earliest planting date is April 11 in most of the state and April 21 in the far northern counties (see the map below for details). For soybeans, the official RMA earliest planting date is April 26 for all Wisconsin counties. If soils warm up and this warm weather continues, some farmers may want to plant before them.
If a farmer chooses to plant earlier than the specified earliest planting date for the insured crop in their county, the crop is still insured and losses will be covered, as long as the farmer follows all the remaining contract specifications. However, the farmer gives up eligibility for replant payments that are part of the standard yield protection and revenue protection included in these combo policies. Specifically, if a crop stand is damaged early in the season so that the projected yield is less than 90% of the yield guarantee, a farmer can receive an indemnity for part of the actual cost of replanting. The affected area must exceed 20 acres or 20% of the unit's acreage. The maximum indemnity is the chosen price election multiplied by the 20% of the yield guarantee, up to 8 bu for corn, 3 bu for soybeans and 1 ton for corn silage. This year, based on crop insurance prices of $5.68/bu for corn and $12.55/bu for soybeans, these allowances imply a maximum replant payment of $45.44/ac for corn and $37.65/ac for soybeans. Farmers who plant before the earliest planting dates give up their eligibility for these payments if they were hit with a late killing frost or similar problems that would normally trigger replant provisions. Note that the yield guarantee is not reduced for late planting reduction if a farmer replants crops under policy replant provisions, so the crop has the same production guarantee as for the original plant date. Farmers with additional questions should contact their crop insurance agent.
If the warm weather continues, growers will have to weigh the risks and benefits of planting early. For farmers with crop insurance coverage, which is most Wisconsin farmers, one of the risks of planting early to consider is the loss of replant coverage.


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I can't think of a better way to begin the new year than by honoring Our Lady. A contradiction to the way the world rings in the new year, we Catholics welcome her in prayer and turn the page of the calendar imploring her blessing and intercession.
She is a role model for us all – male and female alike. I owe so much to her…honestly, without her in my life, I would have been lost.
When I was 27 years old, my husband and I began the struggle of infertility. It was during that time of great sadness and suffering that I started to pray the rosary. Unbeknownst to me, both of my sisters were dealing with their own trials, and at the same time we all began to pray the rosary. I'm not sure exactly how we became aware of this simultaneity, but we all were amazed and thought it was pretty cool. For all three of us, this was a turning point in our lives, the beginning of an amazing journey.
I will tell you what I've said many times when I teach: I don't know what it is or why it is, but something happens when you pray the rosary. If you don't already pray the rosary daily, I exhort you to make it a commitment for the new year. Get up a little earlier and pray it with your morning coffee. Pray it in the car on your way to work. Download it onto your iPod or phone or computer then play it an pray it while you're making dinner or doing the dishes or going for a walk. There are so many ways you can pray the rosary. All you have to do is decide to make it a priority. I can tell you from my own experience, you will not regret it.
For those of you who do pray the rosary daily, keep it up. Try to go deeper with it. Center yourself and pray away any distraction. Read (or re-read) St. Louis de Montfort's Secret of the Rosary. Invite your children or your husband to join you.
By the time she died, my 92-year-old grandmother prayed 27 rosaries a day. "Well, I can't do much, but I can pray!" And pray she did! I called her my prayer warrior. She once lamented to me that one day she was apologizing to Our Lady for the quality of her rosary – so full of distraction and inattention. My grandmother, however, felt reassured by what she believed was Our Lady's reply, "I use every crumb."
Won't you offer her your crumbs, too?
O Mary, conceived without sin, pray to us who have recourse to thee! Anne


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No matter what you want to do in your yard, any landscaping you want to do will require a little knowledge. If you do not understand the process, you are likely to cause yourself a big headache; this article is full of advice to help you get your landscaping project off on the right foot.
Use native plants when fixing your yard. Native plants are easier to care for because they are already comfortable in your climate and soil. This will make them easier to care for and make your landscaping maintenance easier. Go to your local gardening store to learn more about native plants in your specific area.
Buy your products online and in bulk to help save money. Lots of websites exist that offer great merchandise at appealing prices. Be sure to view customer's reviews to ensure the product you are ordering is one of good quality. Remember to compare prices of different websites.
Those of you interested in landscape design for your own home would be smart to use mulch for your flowerbeds. Mulch is useful for keeping things moist, particularly in areas of excessive heat and sun. Mulch helps your plants get as much moisture as they need.
Consult a couple of professionals prior to starting your projec, whether you are inexperienced or not, they can offer valuable advice or caution you away from a mistake.
Re-doing your landscape is like remodeling a room inside your home, and a small amount of knowledge can take you a very long way. Read a lot about landscaping whenever you can, and practice what you learn during your landscaping efforts. This can help you to create beautiful landscapes that you can be proud of for a long time.


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Clinical training
While PGY-1 and PGY-2 residents operate every other day like their senior resident colleagues, training during PGY-1 and PGY-2 is weighted to preoperative and postoperative patient care.
PGY-1 rotations include trauma, critical care and general surgery; vascular surgery; orthopedic surgery; plastic surgery; and rotations with general surgery staff and chief residents.
In PGY-2, you serve as the senior resident in the surgical intensive care unit and have rotations in general surgery, colon and rectal surgery, endoscopy, anesthesiology, gynecologic surgery, and pediatric surgery. Five weeks of elective time is often used to experience cardiac, general, or other subspecialty surgery areas.
During PGY-3 and PGY-4, your training emphasizes surgical technique and skill refinement with rotations that include transplant surgery, vascular surgery, thoracic surgery, colon and rectal surgery, endocrine surgery, and hepatobiliary surgery. PGY-4 residents lead our trauma, critical care, and general surgery team to care for critically injured patients coming to Mayo Clinic from throughout the Midwest.
As a chief resident in PGY-5, you lead your own surgical team and assume full responsibility for patient and resident team management.
Rotation schedule
|Trauma, critical care and general surgery
|3 months
|General surgery
|5 months
|Vascular surgery
|1.5 months
|Orthopedic surgery
|1 month
|Plastic surgery
|1.5 months
|General surgery
|4 months
|Trauma, critical care and general surgery
|3 months
|Colon and rectal surgery
|1.5 months
|Endoscopy, anesthesia and elective
|1.5 months
|Gynecologic surgery
|1 month
|Pediatric surgery
|1 month
|General surgery
|5 months
|Pediatric surgery
|3 months
|Transplant surgery
|1 month
|Thoracic surgery
|3 months
|General surgery
|3 months
|Colon and rectal surgery
|1.5 months
|Vascular surgery
|3 months
|Trauma, critical care and general surgery
|4.5 months
|General surgery
|7.5 months
|Vascular surgery
|3 months
|Colon and rectal surgery
|1.5 months
During PGY-3, you may elect to spend six weeks at one of two off-site rotations — managing pediatric surgical patients at the Children's St. Paul Pediatric Hospital in St. Paul, Minnesota, or caring for general surgery patients at Mayo Clinic Health System — Franciscan Healthcare in La Crosse, Wisconsin.
Both sites reside an hour from Rochester by car and have numerous busy surgeons (eight in St. Paul and four in La Crosse) in a friendly environment void of other residents or fellows. Mayo Clinic funds the authorized additional costs of housing and licensure fees for these rotations. Our residents consistently rate these rotations as highly educational.
Didactic training
Clinical conferences, seminars, small discussion groups, journal clubs, and one-on-one instruction are integral parts of the General Surgery Residency. All residents are required to attend our weekly morbidity and mortality conferences and Monday evening Grand Rounds. Friday morning sessions are held every other week in both simulation and surgical skills centers to enhance resident learning.
During each subspecialty rotation, you also attend that subspecialty's weekly schedule of journal clubs, didactic presentations and conferences dealing with patient management problems, mortality and morbidity.
During PGY-2 and PGY-3, you meet monthly throughout the academic year for journal club, where you learn how to critically analyze and interpret scientific articles.
You are encouraged to attend regional or national general surgery meetings sponsored by Mayo Clinic, and most residents attend the Minnesota Surgical Society meeting one or more times in the five-year period.
Other didactic training during the residency includes:
- Surgical basic science program. You receive basic science instruction in topics such as wound healing, immunology, infections, and organ system pathophysiology. All residents are expected to attend this weekly session held on Monday afternoons before the chief resident conference.
- Trauma, critical care, and general surgery. Your PGY-1 trauma, critical care, and general surgery rotation includes formal lectures as well as daily informal sessions on any number of topics in trauma and critical care. Ten Mayo trauma, critical care and general surgery staff members (board certified in both critical care and general surgery) actively participate in educating residents and students about all facets of trauma and critical care.
- Surgical critical care program. During PGY-1 and PGY-2, your surgical critical care rotations include daily learning about the fundamentals of critical care management. Mayo Clinic has a high volume of tertiary care patients, so you have broad exposure to nearly all aspects of critical care. Additional senior-level rotations in cardiothoracic, vascular, pediatric and general surgery allow residents to mature their intensive care unit skills and care over the ensuing three years.
- Advanced Trauma Life Support certification. As a PGY-1 resident, you have the opportunity to become certified in the American College of Surgeons' Advanced Trauma Life Support program. A recertification course is offered during PGY-3.
Research training
Research opportunities at Mayo Clinic are outstanding. Your particular project(s) depend on your interests and background. Research opportunities are divided into two broad categories — clinical research and basic science laboratory research.
Clinical research
You have access to Mayo Clinic's world-renowned medical records system for clinical research. During your residency, you conduct at least one clinical research project, publish the results, and make at least one regional or national presentation.
Basic science laboratory research
If you have an excellent clinical record and are interested in an academic surgical career, you are encouraged to pursue basic science laboratory research. You may begin a research project after PGY-2, or you may complete your residency training and then focus on research. Roughly one-third of our general surgery residents add one or more years of laboratory research to their five-year residency training. Credits can be applied toward an M.S. or a Ph.D. at Mayo Clinic Graduate School of Biomedical Sciences.
Mayo Clinic offers two basic science laboratory research opportunities: a one-year program and a two-year clinician-investigator program.
One-year research program. This program gives you the opportunity to assess your aptitude for bench research and develop your fundamental research skills. To enhance your productivity, you are encouraged to initially base your research on an existing research project. Current areas of basic science laboratory research include:
- Cardiac surgery
- Colorectal physiology
- Gastrointestinal physiology
- General thoracic surgery
- Laparoscopic surgery
- Oncology and immunology
- Pediatric surgery
- Plastic surgery
- Transplantation, including xenotransplantation, heart and lung, hepatic, and renal and pancreas
- Vascular surgery
Clinician-Investigator Training Program. Mayo Clinic's Clinician-Investigator Training Program is two years in length. When you complete this program, you will be academically prepared, competent in clinical surgery, technically skilled in research and capable of competing in today's research environment.
The Clinician-Investigator Training Program includes two years in basic science laboratory research and a core curriculum of research seminars, guest seminars and didactic courses in subjects such as:
- Advances in cell molecular biology
- Cellular and quantitative biology
- Physiology
- Statistics
If you are interested in the Clinician-Investigator Training Program, you should indicate your interest early in your residency training. You will then be assigned to a faculty member who can help you develop a competitive written research proposal.
Teaching opportunities
You have the opportunity to supervise and teach Mayo Clinic Alix School of Medicine students and visiting student clerks through bedside instruction, operative interaction, and formal didactic lectures.
In addition to clinical experience, didactic training and special courses, Mayo Clinic offers a wide variety of professional conferences and online learning. Whether as part of Joint Commission regulations or Mayo Clinic School of Graduate Medical Education recommendations, dozens of online modules teach current best practices for infectious diseases, professionalism, record keeping and more.
The main department teaching conference is weekly General Surgery Grand Rounds, which is a common meeting ground for all of the subspecialties within the Department of Surgery. Attending consultants openly critique case presentations by chief residents who are assigned to the general surgery, vascular surgery, and colon and rectal surgery services. Additionally, senior-level residents in pediatric, thoracic, transplant, and trauma, critical care and general surgery present their operative lists for scrutiny and education. After discussing the management issues involving patients on such services, staff members make a formal didactic presentation on an area of their expertise.
Monthly video teleconferences with staff and residents from Mayo Clinic's campuses in Phoenix/Scottsdale, Arizona, and Jacksonville, Florida, in general, endocrine and vascular surgery provide education for staff and residents about diverse and difficult surgical problems.
Additional conferences, which include staff pathologists and radiologists, are held monthly to educate surgical residents in managing all facets of patient care, with preoperative, intraoperative and postoperative lessons all being taught.
You are required to become certified in Basic Life Support (BLS) and Advanced Cardiac Life Support (ACLS). ACLS courses are held during the last week of June, just before the start of the academic year. An evening ACLS course also is offered every three months to facilitate recertification. You must be certified in BLS and ACLS before you begin your trauma, critical care and general surgery rotations.
Case studies
During your residency, you frequently prepare case study presentations. You present pertinent information from an interesting case and conduct an in-depth discussion of that case using evidence-based material.
In-training examinations
Each year, you take the written American Board of Surgery In-Training Examination (ABSITE). During PGY-4 and PGY-5, you participate in oral clinical examinations given by Mayo Clinic's surgical staff. These mock oral exams use the same format as the oral certification examination given by the American Board of Surgery.
Committee assignments
You are given an opportunity to gain experience in a number of administrative capacities during your training. Class representatives meet with the General Surgery Residency Committee each month to improve the surgical program. Additional committees led by residents focus on improving all facets of resident training, from camaraderie to laparoscopy and robotic experiences.
Moonlighting is permitted for licensed residents only when you do not have clinical responsibilities, such as during research time or while you are on vacation.
To ensure that you acquire adequate knowledge and develop your technical skills, your performance is monitored carefully during the course of the General Surgery Residency.
Your supervising faculty member formally evaluates you after each clinical rotation. Each evaluation is reviewed carefully by the program director. Electronic access allows you to develop a portfolio of evaluations, curriculum vitae, educational competence and academic effort that will be yours upon graduation.
Annual reviews of each resident at our General Surgery Residency Committee meetings occurs, and your performance in all aspects of surgery must be satisfactory at each level of training before you will be promoted to more-advanced levels. Mayo goes to great length to help struggling trainees improve their performance, and a track record of graduating chief residents successful in fellowships or private practice is testimony to a wonderful system of learning.
In addition, you regularly evaluate the faculty and our program to ensure that your educational needs are met.
Career development
You meet periodically with various faculty members, administrators and the program director to discuss your individual career goals. Mayo Clinic recruits many of its staff physicians from its own training programs, so when you successfully complete your general surgery training, job opportunities may be available at one of Mayo Clinic's group practices.
Operative experience
The operative experience of residents completing the General Surgery Residency Program is well within the guidelines of both the Residency Review Committee for Surgery of the Accreditation Council for Graduate Medical Education and the American Board of Surgery.
For example, the average total numbers for 10 residents who finished the General Surgery Residency were:
|Major operations
|As chief resident
|228 operations
|As junior surgeon
|793 operations
|As teaching assistant
|16 operations
|1,037 operations
Additional training
After you successfully complete the General Surgery Residency, you will be highly competitive for fellowship training programs at Mayo Clinic and throughout the United States.
Mayo Clinic offers fellowships in many surgical specialties that complement the General Surgery Residency.
These fellowships offer in-depth, daily, one-on-one training with a consultant and the opportunity to increase your surgical, supervisory and administrative skills. During your residency, you can talk with your faculty adviser about these opportunities.


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Certain Sermons or Homilies Appointed to be Read in Churches (1547–71) comprises two volumes of religious sermons or homilies published during the reign of Elizabeth I. The homilies are in English and were intended to ensure that lay people understood their religion better. Before the English Reformation, mass was conducted in Latin, which the majority of the congregation couldn't understand.
Sermon 6. 'Agaynst excesse of apparrell'
This homily preaches against overindulgence, vanity and display in one's clothing, which is presented as a threat to morality and social order. It refers to laws of the time trying to control what people could wear. The homily criticises lewdness in dress (particularly in women) and condemns those who cultivate worldly luxuries rather than a spiritual and moral character. It also expresses concerns about 'self-fashioning', criticising the way that people who dress above their station can use clothing to make themselves socially mobile.
Sermon 21. 'An homilee agaynst disobedience and wylfull rebellion'
This homily, explicitly commissioned by Elizabeth I, is a polemical text arguing for the Divine Right of Kings, which is the idea that a monarch has complete authority to rule because he or she is appointed by God. Those who rebel against the monarch, rebel against God. Rebellion is presented as breeding disorder, moral corruption and sin among the populace, and misfortunes (such as poverty, sickness and death) are seen as the result of disobedience. The homily suggests that rebellion against a monarch is as 'unnatural' as a son rising up against a father or a father disinheriting innocent children. Again there is a great fear of disruption to social order.
The homilies develop the reformist doctrine expressed in the Thirty-Nine Articles of Religion that were developed between 1536 and 1571 and which defined the Church of England. The first volume, published in 1547, was mostly written by Thomas Cranmer, Archbishop of Canterbury, with its 12 sermons focused on God and Justification by Faith. The second volume (from which these excerpts are taken) was published with 21 sermons in 1571, and was mostly written by John Jewel, Bishop of Salisbury. Its contents focused on living as a Christian. The homilies in both volumes are still mandated to be read in churches today.
- Full title:
- The Second Tome of Homilees, of such matters as were promised, and intituled in the former part of Homilees. Set out by the aucthoritie of the Queenes Maiestie: And to be read in every parishe Church agreeably.
- Published:
- 1571, London
- Format:
- Book / Quarto
- Language:
- English
- Creator:
- Church of England, John Jewel
- Usage terms
- Public Domain
- Held by
- British Library
- Shelfmark:
- 4455.b.12.
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L.A. Tech Deal Flow: June 26
A weekly roundup of must-read L.A. tech investments and acquisitions.
Part of Match Group's IPO
Type: Public stock sale
Location: West Hollywood
Investors: General public
Description: New York's IAC/InterActiveCorp plans to spin off its Match Group dating division, which includes Tinder, into another public company in the fourth quarter. IAC said it would sell a 20 percent stake in Match Group as part of the offering. Pricing and volume of shares have not been announced. Tinder's financials are not disclosed, but the dating app has added revenue streams lately, including paid subscription features and advertisements.
Related: IAC to IPO Tinder Parent Match Group
$75 million
Type: Equity
Location: Venice
Investors: Led by Technology Crossover Ventures, with participation from Dragoneer, Comcast Ventures and Forerunner Ventures.
Description: Less than nine months after it raised a $50 million investment round, Dollar Shave Club has just raised $75 million more in a Series D round. The round values the company at $615 million, the Wall Street Journal reported. Dollar Shave Club reported revenue of $64 million last year, up from $19 million a year earlier, and estimates it will have sales of $140 million this year. It is not profitable.
Related: Dollar Shave Club Reloads on Cash
$5 million
Type: Equity
Location: Los Angeles
Investors: Slow Ventures, March Capital and Pritzker Capital, with angel investors Paul Jacobs, James Lassiter, Stephen Rader, Jamie McCourt, Lionel Richie and Mike Wortsman.
Description: Heal is an app for ordering house visits by doctors. The app allows users to book appointments, track doctors en route and rate their service. Doctors show up in less than 60 minutes for about $100 a visit.
Related: App Has Line On House Calls
$75 million
Type: Venture fund raise
Location: Venice
Investors: The James Irvine Foundation, Top Tier Capital and others
Description: The new CrossCut 3 fund aims to make 25 to 30 early stage investments over the next two to three years, with 80 percent going to L.A. companies. The fund has already invested in MobCrush of Santa Monica, smart watch app development studio Little Labs of Venice and in-home tech support startup HelloTech of West Los Angeles and said its focus will include SaaS, e-commerce, ad tech, gaming, mobile app and online marketplace companies.
Related:CrossCut Raises $75 Million to Invest in Early Stage L.A.
Technology reporter Garrett Reim can be reached at firstname.lastname@example.com. Follow him on Twitter @garrettreim for the latest in L.A. tech news.
For reprint and licensing requests for this article, CLICK HERE.
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Whatever the season, the I.Code collection of parkas and jackets adds warmth and style to any look. Mademoiselle I.Code's coats are casual or smart to suit everyday needs and give her endless options for different looks.
Dare to opt for printed jackets to give your look a resolutely trendy and imaginative touch. We also love the feminine blazers for elegant outings, worn over a pretty black dress or a women's top and denim jeans for a confident sport-chic look. The more inspired can dare to wear layers, which give the look a bold, sophisticated feel.


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By Erica Dobecki
"People disappear all the time. Ask any policeman. Better yet, ask a journalist. Disappearances are bread-and butter to journalists… Many of the lost will be found, eventually, dead or alive. Disappearances, after all, have explanations. Usually."
In 1991, Diana Gabaldon began a beautifully penned series called Outlander. The series came to prominence this summer when it became a television series of the same name on Starz. The series is historical fiction and is based on the life of main character Claire Fraser and her way of dealing with the time crossover when she goes back in time from 1945 to 1743.
"Now I was alone, next to a sleeping stranger with whom my life was inextricably linked, adrift in a place filled with unseen threat."
Claire Randall. In one moment, her life was turned upside down and it all happened during what was supposed to be a happy honeymoon with her husband, Frank. One minute Claire was in the post wartime 1940s and the next minute, she had tumbled through time and landed in full on war action 1700s Scotland.
The young woman has many transformations from her original state of mind when she was in the 1940s and married to Frank Randall. A lot of Claire's actions are to save herself, such as marrying Jamie Fraser and becoming Castle Leoch's designated doctor.
The transformation of Claire to wife of Jamie is quick but it is not too quick for the audience. Claire and Jamie seem to have been meant to be together, simply by their exchanges. "'There's such a thing as justice, Claire. You've done wrong to them all, and you'll have to suffer for it.' He took a deep breath. 'I'm your husband; it's my duty to attend to it, and I mean to do it.'"
Claire has a difficult time juggling all of her roles as wife, doctor, Sassenach (outlander), and 1940's woman.
The novel often sees Claire trying to juggle her many roles, without getting too overwhelmed.
Historically, Gabaldon touches upon everything that Scotland is famous for during that time period. The biggest thing at that time was the bad blood between the Scots and the English, which are the biggest skirmishes Gabaldon talks about in the book. Gabaldon also touches upon the magical mythologies Scots told and the Gaelic language. The amount of research that was done for this novel alone must have been mind boggling.
Diana Gabaldon has three degrees in sciences, including Zoology, Marine Biology, and a Ph.D. in Quantitative Behavioral Ecology. She was a university professor for over twelve years before beginning her current career and writing fiction novels. She currently lives in Scottsdale, Arizona with her husband and three adult children.
Diana Gabaldon's personal website has information about her tours, various interviews, and her novels, including the Outlander series and many more. The website dedicated to the show is all about the series, including teasers for new episodes and information on the actors. Outlander can also be purchased here, along with the many other novels in the series.


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|PT Kepler 22b
|A regular Pollination Technician like any other, sometimes, while in the midst of probing yet another Sim, PT Kepler can't help but wonder if there's something else out there for her. Something quieter, peaceful, free of the screams of innocent sims but maybe full of little offspring screams instead...
|Type: Alien
|Gender: Female
|Age: Adult
|Aspiration: Popularity
|Zodiac Sign: Cancer
|Marital Status: Single
Unborn Babies Spurlock (Children)
Hair Colour: Blonde
Eye Colour: Black
Skintone: Alien
|Playability: Deceased
|Neighborhood: Sedona
PT Kepler 22b is one of the four deceased alien pollinators in Sedona. It's not known why she and the other aliens died but the flyover video suggests that their UFOs crashed and that could be the reason why they died. Her gravestone can be found in Green Stonehenge.
Shortly before PT Kepler died, she had impregnated Jared Spurlock who is currently pregnant with his unborn twins.
PT Kepler 22b is a full alien (100% alien DNA), so her children will most likely inherit her alien skintone/eyes and will be half-aliens (50% alien DNA).
Note: PT Kepler 22b is NOT a real pollinator. Meaning the game will always choose the real pollinator instead of her when someone gets abducted by aliens.
She's just a regular alien sim. Unlike EA's pollinator, she's safe to resurrect and play as. It will NOT corrupt the game; nor the neighborhood if she's resurrected and made playable.


text: Guest DuckCommanderSR Posted April 22, 2019 Report Share Posted April 22, 2019 Hey guys, I have two issues I'd like to ask for advice on. The first is anyone on here who's a country person having to deal with the stereotypes of country people interested romantically in their cousins because "they have no other options" which in my opinion is just complete ignorance. Which makes me feel I can't tell anyone I know about my feelings for her. And the second is that she's currently in a relationship. Which I'm supportive of her and just want to let her know I'm there for her. Anyone who read this, thank you and any advice would be greatly appreciated. Link to post Share on other sites


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We have a set of values which we use to support all aspects of our business:
Edge: We understand that sometimes tough conversations need to be had to help deliver results for the business. Our people use their people skills to tackle the difficult issues – they know how to win friends and influence people when they need to.
Execution: We see what needs to be done and make things happen. Our people go the extra mile to drive and deliver results to help us achieve our targets.
Expertise: We are an airport made up of many experts – people who really know their stuff. Our experts are an active and integral part of the team who share their knowledge and experience with others.
Energy: Our business thrives on energy – our people bring the buzz to the airport, they are full of enthusiasm and their positivity is infectious. They help create the culture we want to see around the airport.
Empower: Our people are passionate about developing their own skills as well as the skills of others. They lead by example and motivate and inspire people to do their best every day.
External focus: We pride ourselves on knowing what is going on around us and understanding how we fit into the wider business. We make sure we know how teams can work together to get the job done.


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Late of Taree and formerly of Darwin. Passed away peacefully on Tuesday 11th February 2020.
Aged 62 years
Loving Mum of Michael and Danya. Much loved Nanna of Callie, Ava, Jax and Chloe. Will be dearly missed by her fur babies Betty and Sally. A special lady in the lives of all her extended family and friends.
The relatives and friends of the late Toni Briscoe are respectfully invited to attend her Funeral Service in the Chapel at Manning Great Lakes Memorial Gardens, Pampoolah Road Taree, commencing at 10.00 am on Saturday 15th February. Donations in lieu of flowers to Cancer Research would be warmly appreciated.


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What does 'Bad workers always blame their tools' mean?
"A bad worker always blames their tools" - If somebody does a job badly or loses in a game and claims that they were let down by their equipment, you can use this to imply that this was not the case.
- Category: Profession, work or study
Buy our lists of British English, American English and Animal idioms in PDF format. They're perfect to use offline for reference or for use in class!
See also:
- View examples in Google: Bad workers always blame their tools
- Discussion Forum: English Idioms and Sayings
- Idiom Definition
- Idiom Quizzes


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In an update to the previously-reported standings from the 2015 SwimTheSwan event in Perth, Australia as part of the 2015 BHP Billiton Aquatic Super Series, Christine Jennings has been awarded 5th-place honors, and with it a $2,000 cash prize. Originally, official results had her American teammate Eva Fabian in 5th.
The initial red-flag on the result was that Jennings and another American, Haley Anderson, were nose-and-nose as they hit the finish pad, as was verified by several observers at the race, both neutral and biased. While original unofficial results had Jennings in 5th-place, when the official results were released, Jennings' finish time was around 25 seconds slower than that of Anderson, with whom she finished nearly identically to the naked eye.
The error initially went unnoticed because of those unofficial results, but after a protest and several days of investigation, race organizers awarded 5th-place to Jennings, saying that she finished four-tenths of a second behind Anderson and six-tenths in front of Fabian.
Fabian, who is an amateur athlete still competing at Yale, likely would have been unable to accept the prize money per NCAA rules.


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Cristina Vinhas
Cristina Vinhas, was born in Vila do Conde a small city near Oporto. Made jewelry in CINDOR, Centro de Formação Profissional da Indústria de Ouriversaria e Relojoaria, during three years. She started to make there own pieces, and create collections to the company Lucente Jóias, being those collections presented in Portojóia fair.
Development of nature forms, visualization of images or movements or even materials, is what inspires her. To find a harmony and the equilibrium of forms, is what motivate her to create jewelry.
OUR FAMILY – 16th Anniversary Collective Exhibition
28th August — 21st September, 2019
The Collective Exhibition will be held in Creative Macau starting from 28 August to 21 September, 2019. 29 members create a structure of the "family" as an artistic project; and compose a family portrait through the real and, or, within imaginary conception. From the abstraction to the substantial, we have been naming themes for our […]
Open Future – Collective Exhibition
28th August — 22nd September, 2018
"Contemporary life is one that undergoes constant transformation, in the context of spaces, time or memory; we live with a heave, being hemmed in by each other and heaped praise on one another." Creative Macau will be celebrating its 15th anniversary on 28 August 2018 with "OPEN FUTURE" – Collective Exhibition. Our creative members are […]
"Here & Now" 13th anniversary members exhibition
27th August — 30th September, 2016
Adalberto Tenreiro, Alexandre Marreiros, Armelle de L, Chan Un Man, Cristina Vinhas, Duarte Esmeriz, DWG, Elói Scarva, Fernando Simões, Francisco Ricarte, Ho Si Man, João Miguel Barros, Lai Sio Kit, Laura Che, Li Li, Lúcia Lemos, Madalena Fonseca, Marina Carvalho, Mavin Zin, Noah Ng, Ohi, Rodrigo de Matos, Rusty Fox, Sofia Bobone, Tang Kuok Hou […]
"RICH LIFE" – 12th anniversary group exhibition
28th August — 30th September, 2015
Creative Macau (CCI) will hold a grand opening ceremony of "Rich Life" Members Exhibition and bring stunning music performance to celebrate its 12th Anniversary on Friday, 28 August, 2015. The grand opening ceremony will start at 6pm with a set of live acoustic and electronic music played in a combination of different instruments by the […]
"Symbols In Cultures" 11th anniversary group exhibition
28th August — 13th September, 2014
SYMBOLS IN CULTURES Everywhere we look in societies are forms of symbolic representation, which identify and illustrate different culture, philosophy, and lifestyle. Thinking that symbols in society are unconscious forms of communication in the way of social, ideological, political concepts and many others, they are important and integral elements in understanding a culture and a […]
"Make A Wish" 10th Anniversary Group Exhibition
28th August, 2013
MAKE A WISH is the theme of our 10th anniversary exhibition proposed to all Creative Macau members. Twenty six members across the twelve different creative fields of advertising, architecture, craft, design, designer fashion, film and video, interactive leisure software, music, performing arts, publishing, software and computer development, and visual arts were interested and are displaying […]
"Nature" – Jewellery
11th — 31st January, 2013
"Nature" is Cristina Vinhas's first solo exhibition in Macau. Her biggest motivation is to create unique shapes. This collection was inspired from the texture and shapes of organic elements such as coral, ginseng root, pork organs, tree trunks, dried roots, seeds of the lotus flower, trees, birds; as well as existing objects with a different […]


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Lakefront Trail Improvements Underway
Chicago's beloved Lakefront Trail will get a little better this season as the city continues improvement projects through the summer. About 4 miles of trail with separate lanes for cyclists and pedestrians are already open, with plans to add 14 more. And construction on the Navy Pier Flyover, a bridge over Illinois and Grand Avenue, is ongoing.


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Brick House Antique Center
- 247 E Main Street
- Palmyra, NY 14522
- (315) 597-3883
- Visit Site | E-Mail
Brick House Antiques specializes in collectible antiques at affordable prices. We strive to offer the best customer service and prices available. We invite you to browse through our selection of unique antiques for some antique decor that fits your style. You won't find affordable antiques that are quite as elegant and aesthetically pleasing as our variety, so stop by today.
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With summer in full swing, there's no better time to take a leaf out of every New Yorker's book and jet off to a holiday home.
Australia is filled with dreamy summer holiday rentals, from houses that are literally across the road from the beach in Byron Bay to million dollar mansions with pools and tennis courts in Sydney. If you're looking for holiday home inspiration, these holiday homes to rent will ensure you finish summer off in style.
1. Q1 Presidential Penthouse with Private Pool, Gold CoastThe best Gold Coast holiday rentals are typically apartments by the beach, and there are few better places to spend a holiday than in the iconic Q1 building.
Located on level 74, this four bedroom presidential penthouse boasts floor-to-ceiling windows and glass walls, with sweeping views of the Pacific Ocean, the Gold Coast city, and the Hinterlands.
Designed with minimalist decor, Q1 Presidential Penthouse offers up an opulent holiday that epitomises Gold Coast luxury living.
In addition to the four bedrooms with ensuites, and living and dining areas, there are two open-roof balconies that are perfect for lazy days lounging indoors, or entertaining in the evening.
The apartment also features a Swedish sauna, private theatrette with surround sound, and a swimming pool 225 metres above ground level.
Plus, if that's not enough, guests can also use Q1's resort facilities, which include lagoon pools, gymnasiums, and a private cinema and games arcade. To top it all off, Surfer's Paradise is just a short walk away.
2. Feathertop Chateau, VictoriaVenture away from classic beachside holiday rentals with this luxurious villa in the heart of Victoria's vineyards.
Located in the heart of Victoria's magnificent snowy mountains, Feathertop Chateau features a sleek contemporary design made from natural materials including steel and stone.
The property has four bedrooms and houses up to 10 people, and includes open plan living spaces, a kitchen with a stone pizza oven, an outdoor pool overlooking the mountains, and a helipad - just in case you're thinking of flying in from Melbourne.
In addition to the sleek property decor, Feathertop Chateau offers guests a plethora of unique experiences to enjoy in summer, including wine tastings, wine-making experiences, and an in-room spa service.
3. Sentry Rock, Hawkesbury RiverWhat better way to spend summer than in the great outdoors? Perched on the edge of the Hawkesbury River, Sentry Rock is a six bedroom lodge retreat that exudes natural charm in one of New South Wales' favourite locations.
The lodge is located in the heart of an expansive landscape with forestry, lawns, and waterfalls, and offers guests an elegant rustic design with stone and timber walls, and antique furniture.
Spend afternoons strolling through the massive property grounds, or head out on the river for a fishing trip during your stay.
The balcony and outdoor pool provide sweeping views across the river, making it an ideal spot for lazy afternoons spent with good wine and good company. Sentry Rock also has a jacuzzi and steam room, and a wine cellar carved into rocks.
4. Bedarra Island Villa, QueenslandNothing says summer luxury quite like an exclusive island. Just off the Great Barrier Reef, Bedarra Island Villa is a two-acre estate on the water's edge, with a secluded beach just a few footsteps away.
The villa's interiors are designed by award-winning architect Robert Davidov, and includes open plan living with bamboo panelling and handmade bamboo furniture. The three spacious bedrooms all feature king beds with linen sheets, and the open plan living and dining spaces that flow on to the large balcony.
Bedarra Island Villa comes complete with kayaks, snorkelling gear, and fishing equipment for guests to take advantage of the beautiful natural surroundings. And a bonus: the property also features an outdoor hammock, perfect for snapping and sharing on Instagram.
5. Lavender Bay Waterfront, Sydney
Lavender Bay Waterfront boasts one of the best views of any rental property in Sydney, with striking panoramic views of the Sydney Harbour Bridge and Opera House.
Sprawled over two levels, this beachfront property offers six bedrooms, as well as a living room with shutter doors that open onto a balcony facing the Harbour Bridge.
Modern decor and artworks give Lavender Bay Waterfront a light and spacious feel, and the outdoor dining is well placed for evening barbecues or breakfast with a view.
Guests can choose to take a dip in the outdoor pool, or walk to the neighbouring Sirius Cove or Whiting Beach to go for an afternoon swim.
As a bonus, the home is ideally located near Sydney's iconic summer sights: Taronga Zoo, Wendy's Secret Garden, and Balmoral Beach are just a short drive away.
6. Mykonos House, Noosa Heads, New South Wales
With palatial European architecture, landscaped gardens, and an in-ground pool and spa, Mykonos House is a stunning choice for anyone looking for Noosa holiday rentals this summer.
Situated near Noosa's iconic Sunshine Beach and Noosa National Park, this holiday house has six bedrooms, a living room with a dome ceiling and opulent chandelier, sandstone tiles and bamboo flooring throughout.
The expansive Mediterranean terraces are perfect for relaxing with a good book or a glass of rose, and for those who truly want to feel like royalty, the Tower room has breathtaking sea views.
Outside, the sun-warmed pool is surrounded by palm trees and lush gardens, transporting guests to a tropical paradise in the Greek Islands. Truly la dolce vita.
7. Hercules Byron Bay, New South Wales
With the name Hercules and its enviable clifftop location on Byron's 'Golden Mile', this property is one of the best Byron Bay holiday rentals for the summer.
Hercules Byron Bay offers five luxury suites, complete with king-sized beds, walk-in wardrobes, and bathrooms finished with Italian marble.
The spacious open plan living areas flow into the gourmet chef's kitchen and outdoor entertaining verandah with views of the gardens and the Pacific Ocean.
Of course, summers are made to be spent outdoors. The infinity pool is the perfect spot for a dip, and if you're feeling active, the property even has a tennis court on the grounds.
And, if you're dreaming of transforming your own home into a summer oasis, take a look at our article here for tips to create your own Hamptons-style house.


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Actor Peter Fonda has died at 79 after respiratory failure from lung cancer, according to multiple reports.
Best known for his role in 1969's "Easy Rider," Fonda was part of a family of Hollywood heavyweights that included his dad, Henry Fonda, sister Jane Fonda and daughter Bridget Fonda.
He shared writing and producing credits on "Easy Rider," which he starred in with Jack Nicholson and Dennis Hopper. The film's 50th anniversary was on July 14.
He was nominated for an Academy Award for the screenplay, which he wrote with Hopper and Terry Southern.
"[Peter] passed away peacefully on Friday morning, August 16 at 11:05am at his home in Los Angeles surrounded by family," his family said in a statement provided to People. "The official cause of death was respiratory failure due to lung cancer."
"In one of the saddest moments of our lives, we are not able to find the appropriate words to express the pain in our hearts. As we grieve, we ask that you respect our privacy," the statement continued. "And, while we mourn the loss of this sweet and gracious man, we also wish for all to celebrate his indomitable spirit and love of life. In honor of Peter, please raise a glass to freedom."


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"I listen more and react less. I am more aware of when I project onto others, and am forgiving of myself when I do. "
Before I started Kundalini Yoga, my behaviour was, for the most part, unconscious. Although I'd been journeying inward for what feels like my entire life. I was craving a depth that I didn't experience in the Yoga classes I was previously taking and after my first Kundalini class, I felt a powerful shift unlike anything else I'd ever experienced.
However, I was resistant to a regular practice as I was attached to my current way of life, my group of friends and I felt as though the Kundalini community wasn't 'my tribe'. I felt an imaginary expectation from the community for me to conform. I simply wasn't ready.
What eventually grabbed me was the music, the power of mantra and the depth of emotion I would experience after a class. After I attended the Kundalini Yoga festival in Sydney and experienced the live music and the many different walks of life within the community, I felt a richness and a colour that, up until that point, had escaped me.
As I've gained a greater awareness of myself, I've had to make some decisions about relationships that, although I cherish them dearly, are not serving my expression anymore. I've taken a step away from friendships that I've nurtured for over a decade and, at first, that was challenging as I had to grieve the end of a life chapter. It flowed effortlessly once I connected with what feels right for me. I also stepped away from a four year relationship with my partner that I realized was no longer serving me. That was tough as I loved him dearly, and still do.
One of the surprising benefits from my Kundalini Yoga practice is that I've come to know the anger within me more. However, I've realise now that this isn't something to be suppressed. I have learned ways to observe, contain, and direct the energy in a healthy way. I am more accepting of myself during times when anger shows as I can determine the truth of its appearance as opposed to unconsciously projecting it onto others. Anger is now a part of me that needs awareness, like every other part of my psyche.
Other benefits of my practice at Centre for Life include having the opportunity to connect with a lot of people that live with integrity and awareness and, as such, I can engage on a deeper level. Witnessing others' conscious life choices continually inspires me to step up. I have gained tools to be able to develop a daily spiritual practice that works for me and am feeling a lot more balanced as a result. I have an amazing support network where a lot of wisdom is shared and so I feel as though whatever I need, I just have to reach out.
Now I feel much more present when communicating with others. This has allowed my relationships to expand greatly. I listen more and react less. I am more aware of when I project onto others, and am forgiving of myself when I do.
Oh, that's another thing Kundalini Yoga has taught me; to observe without labeling experiences, self and others as 'good' or 'bad'.
Sat Nam.
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Leaf DNS. Keyboard Shortcuts. Robots.txt Wildcards: How to Use Wildcards in Robots.txt. Julian Assange: Choosing Between Trump or Clinton is Like Picking Between Cholera or Gonorrhea. This is viewer supported news Donate Following the end of the Republican National Convention, Donald Trump has received a surge in his popularity.
He's now leading Hillary Clinton 44 to 39 percent in a four-way match-up, according to the most recent CNN poll. Libertarian presidential candidate Gary Johnson received 9 percent, and Green Party candidate Dr. Jill Stein received 3 percent. This is a rush transcript. Visual Composer "How To's" - Visual Composer - WPBakery Knowledge Base. Sell Downloads. Selling eBooks - Join Our ClickBank Affiliate Program. Affiliate Center Affiliate Program Affiliate Links Ads Email Promotion Ebooks Covers Sell our ebooks and earn 50% commission on each ebook!
Join our affiliate program, and start earning money by selling our ebooks through your website or newsletter. We have ebooks teaching creative visualization, affirmations, achieving success, self improvement and positive thinking, as well as peace of mind, emotional detachment, willpower and self discipline, and how to focus your mind. Zip FAQ - Frequently Asked Questions. Free Broken Link Checker - checks your website for bad links / dead links online. SEO SpyGlass – the most powerful backlink checker tool! Protect/Recover your site from Google link penalty with new Anti-Penalty Link Audit feature in SEO SpyGlass.
Low quality, spammy links put your rankings at risk of Google penalty. Whether they result from your wrong link building moves or your competitors' negative SEO, these harmful links need to be instantly detected and neutralized. XML Sitemap Generator online - Free Unlimited Pages. With our free online XML Sitemap generator you can very easy create your sitemap.
First type in your URL and then select the parameters you may wish to change. (change frequency, last modification date and page priority. You may also alter default settings for exclude extensions, do not parse extensions and session ids. Online Sitemap Generator - Free Unlimited Pages. Find SEO Answers. Untitled. Steps to Improve Page Rank and Domain Authority. SEO Analyzer - Web SEO Analysis. Free SERP checker - google ranking check. Serp Rank - SEO Tools, Internet Marketing & Social Media. How to compete on the new Google SERP. How to Feed a Hummingbird: Improve Your On-Page SEO with Related Topics in Moz Pro. Radioactive wild boars rampaging around Fukushima nuclear site. Radioactive boars are running wild and breeding uncontrollably in the northern region of Japan contaminated by the Fukushima nuclear disaster.
The animals have been devastating local agriculture and eating toxic, nuclear-contaminated food from around the accident site. Mass graves and incinerators have been unable to cope with the quantity of boar corpses, shot by local hunters. A quarantine zone near the Fukushima Daiichi nuclear plant where a 2011 meltdown leaked radioactive material into the surrounding countryside has been uninhabited by humans since the disaster. However, boars remained in the area, unchecked by humans. Their precise number is unknown, but since 2014, the number of boars hunted has increased from 3,000 to 13,000, The Times reported. The damage to local farms beyond the quarantine zone caused by the boars has correspondingly increased, amounting to ¥98 million (£620,000) since the accident.
The animals are now being killed faster than they can be buried. How to Use Responsive Images to Make Your Site Shine on Any Platform - The Shutterstock Blog. As internet speeds have improved, we've gotten used to exploring beautiful, high-definition photography on large screens with little to no parameters.
But as the mobile revolution changes the way we interact with the internet, we've had to adjust how these images are served for a host of smaller, differently oriented screens. While responsive design has done wonders for the ways we consume content, it's often been a struggle to correctly display these images across all platforms. Common Problems with Responsive Images Making images "responsive" is actually a relatively simple process — just set a variable percentage width. For example, take a look at the screen shots and code below: While most sites build out full responsive layouts with a few "fluid" rules like this, this simple approach doesn't work for all circumstances — especially when considering the slower download speeds and screen-size ratios of mobile devices.
Child Themes. Languages: বাংলা • English • Italiano • 日本語 • 한국어 • Español • Nederlands • Français • Português do Brasil • Русский • Slovenčina • ไทย • 中文(简体) • 中文(繁體) • Македонски • (Add your language) A child theme is a theme that inherits the functionality and styling of another theme, called the parent theme.
Child themes are the recommended way of modifying an existing theme. How to Create a WordPress Child Theme (Video) Are you looking to create a child theme in WordPress?
Once you learn the WordPress basics, you probably want to learn how to customize your WordPress site. We believe that child themes are a great starting point for anyone looking to customize WordPress themes. How To Create And Customize A WordPress Child Theme. The WordPress platform is a magnet for those who want to take matters into their own hands, who want complete control over their websites and want to be independent in running them.
WordPress does make it really easily to completely customize a website. If you have a bit of knowledge of HTMl, CSS and/or PHP, there is nothing you can't change. I mean, just compare the default themes, Twenty Fifteen81 and Twenty Fourteen2. How To Test Mobile Websites On Desktop: Best Emulators And Tools. Sunday, 06 September 2015 12:10 How to test mobile websites on your desktop with different mobile devices is a question we get quite often.
There's a huge amount of devices out there and you need to make sure your sites work fine for the majority of them. As Google is placing a huge emphasis on mobile user experience – their latest algorithm update causing nearly 50% of non-mobile friendly URLs to drop in ranking, making it bigger than the "Panda" update – you can be sure that if your site's mobile visitors will not like the loading speed and what they see, they will leave, and that will affect your site's ranking. We have collected in this post some of the best free and paid tools that you can use to test your mobile sites on your desktop on various devices without having to actually go and loan or purchase the devices. Just keep in mind that whenever possible, it's always best to do sanity checks on the actual devices as well, if you have access to them somehow. 4) add ? CSS: centering things. See also the index of all tips. Centering lines of text The most common and (therefore) easiest type of centering is that of lines of text in a paragraph or in a heading.
CSS has the property 'text-align' for that: How to Center Images Using CSS. Updated March 24, 2016. If you've ever had to build a webpage, you have likely needed to center something in that page. Using Cascading Style Sheets (CSS) is the proper way to center images, blocks of text, and even your entire layout for a Web page. Most of the properties for centering have been in CSS since version 1.0, and they work great with CSS3 and modern Web browsers. Unwanted Inline styling. Widgetizing Themes. Languages: English • Français • 日本語 • Português do Brasil • 中文(简体) • Македонски • (Add your language) What is Widgetizing? Widgetizing is a pseudo word that describes the process of implementing Widgets and Widget Areas into your Theme. Louis C.K. Goes Off on Trump, Calls Him an "Insane Bigot"
Louis C.K. is far from the first celebrity to openly condemn Donald Trump. Tutorial to create an Animated GIF in Photoshop CS5 CS6 & CC. Let's say you have a folder full of images that you want to sequence together as frames in an animated GIF. You can find special programs online to do this, but with some of the new features of Adobe Photoshop, it's quite fast and simple. Tutorials/Village mechanics. See also: Village and Villager. How I Keep My Villagers Alive... : Minecraft.
Stuff to investigate. Gmail junk. Generic Computer Stuff. WordPress Stuff. Create House Floor Plans Online with Free Floor Plan Software. CSS and Responsive design. Cat info. Pearltrees.


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Good morning everyone. Oh and what a simply beautiful morning it is!! And guess what? Today is Lobster Day!! And why is that? Because it's Uncle Moo's Birthday! For years and years, I would prepare a lobster feast for my Michaael and my brother because they share the same birthday. I have already sent my prayers to the lobster fairy to please bring a nice fat lobster for Moo. (I usually go out and get it myself, but this week I have been out of sorts.)
At the moment, Moo is out with his best friend Joe having a birthday breakfast together. My brother and Joe met back in the summer of 1953. Imagine!! That's FIFTY SEVEN years ago! How many of us are lucky enough to still have your best friend with you after fifty seven years?
And you want to know something else? Joe's birthday was yesterday!!! How is that for fate? You know, sometimes these two remind me of the odd couple. And they do love to bicker. But they are always there for each other. So Happy Birthday Moo, and to you too, Joe. May you have many more special days filled with sunshine and happiness. I love you guys!
I don't have much more news for the moment. Today is my last day to pamper myself and try to get over being sickly. Tomorrow I have to go back to work, ready or not. Oh yuk!! Before I leave you this morning, I would like to share a photo of the view outside my living room window.
My rose bush doesn't bloom very often any more, but my little birdie friends absolutely love it. In it, they find respite from the sun and the rain, a safe place to rest, and a place to meet with their friends and family. I hope you enjoy this photo. I think it's rather fitting for today, don't you? Till tomorrow, Y'all have a beautiful day.
Hugs, Edna B.
"The Meeting Place"


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The commercialisation of the countryside is a key economic history problem. Since we do not know better than that market exchange stimulates economic growth, we need to find out how and when markets penetrated the countryside to stimulate production and increase productivity. When doing that we should conceive markets as being more than institutions for economic exchange. The old, simplistic sequence of
That broader interpretation is still a fairly recent one; ten, twenty years old at most, but it has led to a very important reappraisal of peasant mentalities. Peasants used to be seen as blindly conservative, resisting clear and supposedly favourable economic incentives to change, whereas we now appreciate that those incentives were often outweighed by the importance of networks of social exchange on which their existence depended. As a rule the goods and services exchanged within those networks had a clear price, but they were bought and sold on credit while accounts were cleared without the use of money. As a consequence, transactions and settlements remained invisible to earlier generations of economists and historians, who, taking cash as the hallmark of commercialisation, interpreted such exchange systems as backward barter.
If we accept a broader concept of commercialisation, we face a new problem: what then shapes those socio-economic and socio-cultural networks? Wouter Ronsijn took that question as the lead for his PhD thesis defended in Ghent and it does not surprise that he finds a familiar answer: the social structure and more in particular the distribution of property ownership and access to property determined the way in which those networks operated. If the answer sounds familiar, it does not diminish the value of Ronsijn's meticulous research and of his results, which build up to a superb panorama of the social and economic dynamics on the Flemish countryside between about 1760 and 1910.
For his research Ronsijn focused on two east Flemish regional market towns, St. Niklaas and the somewhat smaller Oudenaarde, and their
Ronsijn split his 150-year timespan into two subperiods, 1750–1825 and 1825–1910. Throughout that period the population in both areas proved highly responsive to changing incentives and opportunities, but not in the same way, because the local social structure shaped their response and that structure differed markedly. The Oudenaarde region was characterized by a highly polarized property distribution, a smallish number of big farms dominating numerous smallholdings. The latter were too small to provide a living, so their holders worked as labourers on the big farms and in the proto-industry, notably the processing of flax into linen. Smallholders consumed all they produced and were usually paid in kind, i.e. produce from the big farms, so they sold nothing on the Oudenaarde market. Nor did the big farms, which tended to export their grain out of the region. As a result the Oudenaarde market had a distinct retail character, selling small quantities of produce directly to consumers.
By contrast, medium-sized farms dominated the St. Niklaas region. Those farms had no need for extra hands, so smallholders had to look elsewhere for work, with interesting consequences. The medium-sized farms sold their produce wholesale on the St. Niklaas market, smallholders worked for cash in the proto-industry or as seasonal labourers in the nearby Dutch part of Flanders, in Wallonia, or further away in France or Germany. Consequently the St. Niklaas region was far more cash-oriented and its market trade livelier and on a bigger scale.
Social structures also determined the response to periodic crises. Oudenaarde proved far more vulnerable than St. Niklaas to the collapse of the linen industry during the second quarter of the nineteenth century, which destroyed the precarious economic balance of the peasants and ruptured the bonds of mutual dependency between large farmers and smallholders so characteristic of the area. By contrast, the St. Niklaas region proved more resilient, people switching from the linen industry to work as seasonal migrants or in the fledgling industries that the more buoyant city attracted. It was only following the great farming crisis of the 1870s and 1880s that the marked polarisation of the Oudenaarde region began to level as big grain growing farms were broken up into smaller holdings and the rise of intensive stock raising opened up new opportunities to smallholders. By that time the rise of new wholesale channels and the food processing industry absorbed most farming output, supplanting the function of market towns like St. Niklaas and Oudenaarde.
Summing up, Ronsijn has produced a highly valuable study detailing the interaction between social structures and commercialisation in the Flemish countryside. I fervently hope his approach will serve as inspiration for similar studies in other Low Countries regions.


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The ancient god's skin was rough and was covered in cracks. However, the hand formed by the violet light was extremely smooth. Although it was huge, it seemed to be formed by a spell.
It made a gesture calling Wang Lin like an elder relative calling a child. The action was slow, but it repeated over and over.
There was no dangerous aura coming from the huge arm, it felt very ordinary. It only beckoned Wang Lin as if it was going to take him through this giant door.
However, Wang Lin was completely on guard and all the hair on his body was standing up. The scene before him was simply too strange. He never would have thought that such a strange thing would occur when the heaven defying bead was complete.
Wang Lin remained motionless as he stared at the hand beckoning him and a cold light flashed across his eyes.
The movement of the arm remained the same, still beckoning Wang Lin silently. However, as time went by, the door behind it gradually turned more illusionary as if it could dissipate at any time.
It was as if he would never have a chance to enter again if he didn't hurry over.
Wang Lin clenched his teeth and stepped forward. His right hand reached out to the only thing that still hadn't collapsed, the Moongazer bone.
Although the Moongazer bone had lost the metal element, it was still as hard as before. After all, this was something that belonged to the Moongazer Serpent!
After grabbing the giant Moongazer bone, Wang Lin let out a roar and celestial spiritual energy surged through his body as he mercilessly threw the Moongazer bone into the sky. The giant bone whistled through the air as it headed straight for the door.
Just as the Moongazer bone was about to hit the door, the hand arm beckoning Wang Lin easily caught the bone.
Wang Lin gasped and his eyes were filled with horror as the arm casually squeezed. A powerful aura immediately appeared and the entire Moongazer bone collapsed into dust!
This squeeze caused the entire world to tremble. Countless cracks immediately appeared on even on the planet Wang Lin was on. Loud rumbles came from the asteroid field as a large number of asteroids shattered.
It created a vacuum within the area, and even the asteroids outside the range of the purple light were affected and continued to collapse.
It was as if this squeeze contained unimaginable power. It was as if the entire world could be shattered by that squeeze.
That arm slowly loosened and beckoned Wang Lin once more.
Wang Lin's scalp went numb and his face was pale as he stared at the arm. He had no idea how strong that squeeze was, but he knew that it wasn't any weaker than the ancient god finger from the Moongazer Serpent!
This arm gave Wang Lin the feeling that nothing in the world could resist that squeeze.
The squeeze didn't use strength but something similar to the law Wang Lin displayed with the Heavenly Chop! However, compared to the Heavenly Chop, this was much more powerful.
After a long time, the giant arm gradually dissipated. However, it maintained its beckoning gesture until the end. The giant door behind it disappeared along with the arm…
The violet light surrounding the area condensed once more. In the blink of an eye, all of the violet light gathered and slowly disappeared, reverting back to the heaven defying bead.
As if it had released all its power, the bead fell from the air. Wang Lin hesitated for a bit before his right hand reached out. The bead immediately flew over and landed in his palm.
It was completely ordinary, as if it was a normal stone. However, at this moment, there was no trace of the five elemental energies from it. It was even more thoroughly depleted than when Wang Lin first found it.
Wang Lin's divine sense went inside the bead. Li Muwan's, Wang Ping's, and Qing Yi's souls were still inside.
Compared to before, they seemed even more lively, especially Li Muwan's soul, which seemed more stable now.
"What exactly is the power of the heaven defying bead… Where does that huge door lead to.. And that arm outside the door…" Wang Lin pondered.
It wasn't hard to guess that he wasn't the first person to obtain the heaven defying bead considering how long it had existed for. There must have been someone else who was able to complete the five elements for the heaven defying bead.
However, after seeing the gate, those people must have chosen to enter, whether it was the first or one of the subsequent times the door appeared.
"Were these seniors crushed by the arm or did they really enter the door…" Wang Lin frowned.
Before the heaven defying bead was complete, Wang Lin was really puzzled by it. However, after the five elements were completed, not only did nothing change, he was even more puzzled.
"Could this door lead to the ancient Celestial Realm… But if that is the case, why is that arm there? Although it looks like it is beckoning, in reality it is there to crush…"
"What exactly is the heaven defying bead…" Wang Lin frowned and stared at the heaven defying bead. After a long time, he placed it against his forehead and it disappeared into his origin soul.
Since he couldn't understand right now, Wang Lin decided to stop thinking about it for now. It was obvious that the key was the door, but he just didn't know what was behind the door!
"The heaven defying bead's five elemental energies are now gone and need to be gathered once more!" Wang Lin looked around. After what just happened, this planet was completely wasted. There was no longer any value in staying here.
"Unfortunately, this treasured place is now gone, but the place that makes my origin soul comfortable is deeper in the asteroid field. If there's more thunder, then I can cultivate the thunder origin spell."
After making up his mind, Wang Lin moved. He charged out of this place like a bolt of thunder. Most of the asteroids nearby were now gone. Wang Lin didn't pause as he took out the war chariot and the Thunder Beast appeared.
The moment the Thunder Beast came out, it let out a roar as if it had been suppressed for countless year and looked at Wang Lin. However, the Thunder Beast was surprised. It carefully looked at Wang Lin and its eyes revealed confusion.
Wang Lin sat down on the Thunder Beast, then he slapped its head and laughed. "Don't stare anymore. With your intelligence, you must have noticed what's special about the place ahead. Are you still not heading over?"
The Thunder Beast let out a roar. In its eyes, it was as if Wang Lin doesn't exist at all, and in his place was a bolt of thunder. However, this bolt of thunder gave him a very cordial feeling, a feeling he hadn't felt in a long time.
Wang Lin's slap on its head made it feel extremely comfortable. It couldn't help but let out a roar.
Thunder Beast was very fast as it traveled through the asteroid field. The deeper they went, the denser the asteroids became. There were even some asteroids giving off flashes of thunder.
This environment caused no discomfort for neither Wang Lin nor the Thunder Beast. The Thunder Beast was extremely happy, and it continued to roar happily.
While sitting on the Thunder Beast, Wang Lin was still thinking about the heaven defying bead. Deep in his heart he was still disappointed. After all, he had spent almost 1,000 years gathering the five elements, and the trip to the Moongazer Serpent almost cost him his life. He originally thought that he would gain some great power that would have been of great use to him.
However, right now the difference between reality and his hopes was too big.
"What exactly is this heaven defying bead…" Wang Lin touched his forehead and pondered.
The Thunder Beast was very sensitive to the power of thunder. It continued to travel deep into the asteroid field.
Muffled sounds of thunder interrupted Wang Lin's thoughts and his eyes lit up. The place ahead was where the comfortable feeling in his origin soul was coming from. The thunder here was extremely dense.
"This is the place!" Wang Lin's eyes narrowed.
Thunder Beast traveled through the asteroid field and then suddenly stopped. Its large eyes revealed shock.
When Wang Lin saw what was before him, he gasped and his eyes started shining brightly.
There was no planet before him, it was a vast lake of thunder. This place was filled with lightning and thunder. It was like a real thunder hell!
The thunder planet from before was really insignificant compared to this place.
In the boundless lake of thunder, lightning bolts moved around like dragons, and the rumbling released a powerful aura.
The Thunder Beast let out a roar and looked at the lake of thunder with hesitation. The power of thunder here was too strong. Even though it was a Thunder Beast, it still almost felt like it couldn't withstand this.
Wang Lin's body moved and he got off the Thunder Beast. His eyes narrowed and became cautious. He first let out the celestial guard to stand guard nearby and then arrived at the edge of the lake of thunder. He observed for a while before taking a step.
When his step landed, the thunder immediately entered his body through his feet and filled his being. Even Wang Lin's body felt numb. He took a deep breath and then took another step.
He took 20 steps in a row before stopping. His entire body was surrounded by thunder and his origin soul was giving off the might of thunder.
This place was his currently limit. Wang Lin slowly sat down and closed his eyes to cultivate.
The Thunder Beast fidgeted around the edge for a long time before finally taking a cautious step forward. It immediately felt the thunder and then lied down. It began absorbing and refining the thunder using a method only the Thunder Beasts knew.
There was no other sound beside the sound of thunder. The man and beast just began to cultivate like this.
Wang Lin gradually adapted to this place, and his heart was in shock. The thunder here exceed that of his body. If it wasn't for the fact that his body was made of thunder, it would have been very difficult for him to even remain at the edge.
"This is indeed the best place to cultivate the thunder origin spell!" Wang Lin's eyes were filled with thunder as he silently cultivated the thunder origin spell.
At this moment, in a distant space outside the asteroid field, a bolt of thunder whistled by. This was a Thunder Beast, and although it had no horn, there was a bulge forming on its head.
The Thunder Beast was wearing black armor, making it look extremely ferocious. A middle-aged man was sitting on the back of the Thunder Beast. His hair flowed behind him and he was expressionless as he charged toward the asteroid field.
"The Thunder Celestial Realm is about to open. I must absorb more origin energy from the thunder lake…"


text:
iStick Power by Eleaf
The iStick Power comes with an ultra-large battery capacity of 5000mAh in a compact design, which can last you for a remarkably long time at any power setting. Not only does it apply the new firmware with Smart mode and custom logo, the iStick Power also has a fashionable looking and comes with many attractive colors for your options.
Technical Specifications
- Thread type: 510 thread
- Output mode: VW/Bypass/Smart/TC(Ni,Ti,SS,TCR-M1,M2,M3) mode
- Output wattage: 1-80W
- Resistance range: 0.05-1.5ohm (TC modes) 0.1-3.5ohm (VW/Bypass/Smart mode)
- Temperature range: 100-315℃/200-600℉ (TC modes)
iStick Power kit includes
1 iStick Power
1 USB Cable
1 User Manual


text: BST-2/tetherin blocks the release of varied enveloped infections including HIV-1 using a physical tethering super model tiffany livingston. cells and transfected 293T cells by American blotting transiently. Endogenous BST-2 in HeLa cells made an appearance being a smear of multiple rings with molecular fat (MW) around 30 kDa, presumably because of N-linked glycosylation (Amount 1B, street 6). The transiently-expressed BST-2 in 293T cells exhibited quicker mobility compared to the endogenous proteins (Amount 1B, lanes 2C5). Stably-expressed wild-type (WT) BST-2 in 293T cells migrated much like the endogenous BST-2 in HeLa cells (Amount 1B, lanes 6 and 7), while glycosylation site mutations (N65A, N92A and N65/92A) decreased the MW of BST-2 (Amount 1B, lanes 3C5 and 8C10). Open up in another window Amount 1 N-linked glycosylation impacts subcellular distribution of BST-2. (A) Schematic representation of BST-2 variants. Glycosylation sites N65 and N92 are noticeable in reddish; (B) Assessment of post-translational modifications of transiently- and stably-expressed BST-2 variants. 293T cells were transfected with 200 ng of BST-2 variants. After 48 h, these cells and stably-transduced cells were analyzed by European blotting using an anti-BST-2 mAb; (C) 293T cells stably expressing BST-2 variants in 10-cm dishes were lysed and analyzed by sucrose gradient ultracentrifugation. Samples were analyzed by Western blotting with an anti-BST-2 mAb; (D) Percentages demonstrated in black and white columns, respectively, displayed the levels of higher-glycosylated or lower-glycosylated and un-glycosylated BST-2 in panel C from three experiments. Levels of glycosylated Ezetimibe pontent inhibitor patterns of BST-2 were quantified from the image J software Results were demonstrated as mean SD; (E) Levels of glycosylated patterns of BST-2 in each sample in panel C from three experiments were quantified and plotted. Results were demonstrated as mean SD. These experiments were repeated three times, and the most representative Western blot images are shown. To investigate the effect of glycosylation within the subcellular distribution of BST-2, we analyzed BST-2 variants having a subcellular fractionation assay. The 293T cells stably expressing BST-2 variants were lysed with moderate sonication to keep up subcellular constructions. The lysates were isolated on a sucrose layer with increased densities by ultracentrifugation. Eleven fractions were collected from the top of the gradient and analyzed for BST-2 by Western blotting. BST-2 N65/92A and BST-2 N92A were recognized as un-glycosylated and lower-glycosylated forms compared with WT BST-2 and were found primarily in fractions TLR1 with bigger densities (Amount 1C,E). In comparison, BST-2 N65A just exhibited a moderate alteration in localization in the thickness gradient. To be able to additional check whether WT variations and BST-2 differ within their subcellular localization, we quantified the percentages of higher-glycosylated or un-glycosylated and lower-glycosylated BST-2 for every BST-2 group. BST-2 N92A, specifically BST-2 N65/92A exhibited generally as un-glycosylated and lower-glycosylated forms Ezetimibe pontent inhibitor weighed against WT BST-2 (Amount 1D). These outcomes suggested which the BST-2 proteins with mutated glycosylation sites Ezetimibe pontent inhibitor would display as lower-glycosylated forms and translocate to subcellular fractions with bigger densities, which might be vesicular compartments apart from the plasma membrane. 3.2. BST-2 Protein with Mutated Glycosylation Sites Accumulate at Intracellular Compact disc63-Positive Vesicles HIV-1 viral contaminants assemble at different sites in various subtypes of web host cells [23]. Nearly all virus contaminants assemble on the cell surface area in T cells and many non-hematopoietic cell lines, while in macrophages these occasions occur almost completely in intracellular membranes which represent a subset of Compact disc63-positive vesicles [24]. Provided the above outcomes, glycosylation are able to be looked at to have an effect on the intracellular localization aswell as the subcellular distribution, like the ER, or Compact disc63-positive vesicles. To verify this hypothesis, 293T cells stably expressing BST-2 or its variants were utilized to detect the co-localization with Compact disc63 and ER-Trackter. As proven in Amount 2A, most BST-2 variations exhibited a puncta-like distribution. WT BST-2 partially appeared in the ER, and BST-2 glycosylation mutants showed similar profiles. In contrast, BST-2 Ezetimibe pontent inhibitor N65/92A was recognized as larger puncta, which co-localized with the CD63-positive compartments (Number 2B). The results indicated the BST-2 mutants lacking glycosylation sites were still able to traffic through the ER membrane but then accumulated in the intracellular CD63-positive vesicles. Open in a separate window Open in a separate window Number 2 Impairment of N-linked glycosylation Ezetimibe pontent inhibitor induces enhanced subcellular distribution of BST-2 in CD63-positive vesicles. (A) 293T cells stably expressing BST-2 or its variants were stained; blue,.


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The Mutual UFO Network (MUFON) today received a report from an eyewitness who shaped the "flying saucer" near the great wall at Simatai in the north of Miyun County, 72 miles northeast of Beijing.
US-based MUFON is the world's largest independent organisation dedicated to UFO, alien and other paranormal research.
It also maintains a database of sightings from across the globe, which it investigates.
The unnamed photographer said in a report to MUFON: "I Went for great wall hiking.