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Figure 1. OFD1 in the centrosome. Centrosomes were isolated from HEK293 cells and analyzed by immunofluorescence microscopy (A through I) and Western blotting (J). For immunofluorescence microscopy, centrosomes were stained with antibodies against OFD1 (green; A) and α-tubulin to label the centrioles (red; B). Merged images of centrosome pairs (C through F) show partial co-localization of OFD1 with α-tubulin at centrioles (arrows in C). OFD1 was also detected in an extended region of pericentriolar region (dotted line in C). Centrosomes from the same preparation were stained with mouse (G) and rabbit (H) antibodies against γ-tubulin followed by Alexa-594 goat anti-mouse (red) and Alexa-488 goat anti-rabbit (green) antibodies. Merged images (I) show perfect overlap indicating no microscopic chromatic aberration. Bar = 2 μm. For Western blotting (J), 10 μg of protein samples from total lysates of HEK293 cells (T) and isolated centrosomes (C) were separated by SDS-PAGE and then either Western blotted and probed with antibodies against OFD1, γ-tubulin, and β-actin as indicated or silver-stained. OFD1 and the known centrosomal marker γ-tubulin were detected in total lysates and isolated centrosome preparation, whereas β-actin was barely detected in the centrosome preparation. Molecular weight markers (kD) are indicated.
Figure 2. OFD1 in the cell cycle. Asynchronous HEK293 cells were processed for indirect immunofluorescence microscopy using anti-OFD1 antisera (green), anti–γ-tubulin antibodies (red) to detect the centrosome, and Hoechst 33258 to stain DNA. For each wavelength, images were captured and processed under identical conditions. OFD1 preimmune serum did not detect centrosomes (A through D), whereas the immune serum stained a large area reminiscent of the PCM in interphase cells (E through H). The field depicted in I through L contains interphase and mitotic cells, and the latter are indicated by an asterisk in their nuclei. In mitotic cells, the OFD1 signal was present but in a smaller, tighter region reminiscent of centrioles (arrows in I and L). Bar = 5 μm. (M) The relative abundance (arbitrary units) of γ-tubulin and OFD1 (measured using two independent antisera, OFD1[a] and OFD1[b]) at interphase centrosomes and metaphase spindle poles was compared. Interphase (I) intensities were quantitated on regions of interest that encompassed the complete centrosome area, whereas mitotic (M) values are given as the sum of the intensities at the two spindle poles. Centrosomes in at least 50 cells were imaged for each condition, and error bars represent SD (P < 0.0001 for OFD1).
Figure 3. Centrosomal targeting of OFD1. (A) Individual GFP-OFD1 fusion proteins and their centrosomal localization (+ [present at the centrosome], +/− [only a subset of transfected cells show centrosomal localization of OFD1], or − [absent from centrosomes]). The yellow ovals indicate LisH motifs; purple boxes indicate the CC domains. (B through D) An example of GFP-OFD1 fusion protein localized to the centrosome (arrows), in this case, a wild-type transfectant is shown, whereas E through G show an example in which the fusion protein fails to localize to the centrosome (arrows in E and G), in this case, a construct Δ5 transfectant. B and E are immunostained with γ-tubulin (red), C and F show GFP-OFD1 green fluorescence, and D and G are merged pictures. Bar = 2 μm.
Figure 4. Electron microscopy (EM) of N70 cells with immunogold labeling for OFD1. Human metanephric mesenchyme cells were fixed and processed for immuno-EM. Gold particles were closely associated with the centrioles themselves (asterisks, A and B). Some gold particle labeling was also detected in the vicinity of the centrioles (within 500 nm), presumably corresponding to pericentriolar material (PCM; C and D). Bar = 500 nm.
Figure 5. Treatment of N70 cells with nocodazole. Control (A) and treated cells (B) were probed with β-tubulin antibody in red, to visualize microtubules (MT), and OFD1 antiserum in green. Note the normal radial arrangement of the MT in A, whereas in B, no MT pattern can be discerned. A discrete OFD1 signal is seen in the microtubule organizing center region in control cells (arrows); after MT disassembly, a comparable OFD1 signal was seen. n, nuclei. Bar = 2 μm.
Figure 6. OFD1 in mitotic cells. Immunofluorescence microscopy was performed on HEK293 cells in interphase (A) and different stages of mitosis (B through F). Cells in A through F are stained with OFD1 antiserum in green and propidium iodide in red to show condensation of chromosomes. OFD1 signal at the spindle poles is indicated by arrows. Bar = 2 μm.
Figure 7. OFD1 in mesenchyme and epithelia. Sections from a metanephros of 7 wk gestational age were stained with propidium iodide in red (A) or OFD1 antiserum (B) or preimmune serum (C) in green. Metanephric structures such as ureteric bud (u), vesicles (v), mesenchyme (m), and stromal cells (s) are visible. (D through L) Greater detail of OFD1 immunostaining (D, G, and J), γ-tubulin immunostaining (E, H, and I), and merged pictures (F, I, and L) of those structures. In the mesenchyme, OFD1 signal co-localizes with γ-tubulin immunostaining (arrows in D, E, and F), as a single dot per cell. In vesicles (outlined by the dotted line in G, H, and I), OFD1 co-localizes with γ-tubulin immunostaining as a punctate pattern in the apical region of the forming epithelium. In a ureteric bud branch (outlined by the dotted line in J, K, and L), OFD1 co-localizes with γ-tubulin staining in a dot-like pattern and is also detected as a diffuse signal in the apical portion of the epithelium. The lumen of the epithelial structures is indicated by l (G through L). In J, crosses indicate individual cell bodies. Bar = 50 μm in A through C, and 10 μm in D through L.
Figure 8. Lectin labeling. A 12-wk metanephros was probed with Arachis hypogea (A and B), Dolichos biflorus, (C and D), and Lotus tetragonolobus fluorescently labeled lectins (E through H). Double labeling with lectins and OFD1 antiserum shows presence of OFD1 in the apical portion of epithelial cells in distal tubules (A) and the collecting duct lineage (C); E shows a tubule labeled with the proximal tubule lectin, and in F, it is apparent that the same tubule expresses OFD1 in a punctate pattern. G shows the merged images of E and F. When preimmune serum was used, no apical signal was detected (B, D, and H). Note that in A and B, the red and green colors have been reversed; therefore, all of the lectin staining appears in green and OFD1 signal appears in red. Bar = 10 μm.
Figure 9. OFD1 in primary cilia. Renal proximal tubule epithelial cells (RPTEC) were stained with OFD1 antiserum (A) and with acetylated α-tubulin to label the primary cilium (B). The merged picture (C) shows localization of OFD1 signal in the basal body of the cilium (arrow) and some dots in the stalk of the cilium as well (arrowheads). Sections from a metanephros of 12 wk gestational age immunolabeled with OFD1 antiserum (D), acetylated α-tubulin (E), and merged picture (F) show that OFD1 is concentrated in the basal bodies of cilia in renal tubules. No signal is detected in the basal bodies when preimmune serum is used instead of OFD1 antiserum (G through I). *Lumen of the tubules. Bar = 0.5 μm in A through C, and 7 μm in D through I.
Table 1. PCR primer for OFD1 constructs


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Originally from Atlanta, Meghan Clark relocated to Charlotte after the birth of her daughter to be closer to family. Meghan loves cooking, and had worked in the restaurant industry for most of her career, but when a friend referred her to the free job training programs at the Goodwill Industries of the Southern Piedmont Opportunity Campus (GOC), Meghan embraced the opportunity to make a change.
"I was really excited and I just decided to go for it, which is not typical of me," she said. "I was just like, I've got to try something different!" She decided to jump right in – on the same day she visited the GOC, she signed up for the Customer, Administrative and Business Services (CABS) training course. "If I was going to make this happen, I had to take some big risks, and I got some big rewards," she said. On the last day of her CABS course, Emily Scott, Sr. Development & Training Specialist, asked for Meghan's phone number, saying that she may have a temporary job opportunity with Goodwill University (Goodwill U). The next day, Emily called, and the next week, Meghan was working in Goodwill U.
On the Goodwill U team, Meghan gained a range of new skills and was ready to look for the next opportunity when this assignment ended. As luck would have it, a team member in Goodwill Industries of the Southern Piedmont's Basic Needs department welcomed her baby right as Meghan's assignment was set to end. Rachel Leach, Job Resource Center Supervisor, asked Meghan to cover this newly opened position while the woman was on maternity leave. In this temporary role, Meghan learned a lot about the services offered by Goodwill® and our partner organizations such as Loaves and Fishes and Crisis Assistance Ministry. To say Meghan was happy to work for on another assignment with Goodwill may be an understatement.
"I really love being here. I love what Goodwill stands for, I love what they do for people," she said. "I've always felt like it was something I could relate to from a personal perspective since I know what it's like to not be happy with your job, not feel like you're making the money you need to support yourself and your family, and need a new direction. So if I can be part of other people's journeys that I went on, I'm all for it."
It's clear she enjoyed her time with Goodwill, because Meghan just transitioned into her third role with Goodwill, this time as a full-time Client Navigator! Meghan now helps new clients register with Client Track and figure out what services would best help them achieve their goals. Meghan's favorite thing about working at Goodwill is how supportive everyone has been. "Coming from the restaurant industry – it's really competitive. Good restaurant jobs are hard to find and there are a lot of people who don't want to work in the restaurant industry, so you work with people who aren't as passionate," she said. "I still love cooking, but here, it's been completely different. I feel like anytime I do something well, everyone is super supportive and happy for me. I feel like I've found a new little family!"
Meghan just started coursework at Central Piedmont Community College (CPCC) toward an Associate's degree in nonprofit management. Her sights are set on completing that degree, earning a Bachelor's degree from UNC Pembroke, and then a Master's degree from UNC Charlotte. "I definitely feel like I've found my calling, and that this is where I need to stay, in nonprofit," she said. "I'm still amazed at how everything fell in line."
When you donate and shop at your local Goodwill, you help individuals like Meghan reach their full potential through the power of work. In fact, 87 cents of every dollar you spend in our retail stores and through shopgoodwill.com® and other e-commerce platforms is reinvested in your community to create employment placement, job training and career support services. Find the location closest to you or make a monetary donation to show your support
Note: As originally posted on Goodwill Industries of the Southern Piedmont.

subset: commoncrawl


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Mar 24, 2009
Sam, Keith, and Doc are joined by cousin Marcus for some Bad Beer, Insignificant Stats and Facts, and some Doc News. A new listener who went to high school with Doc sends in some questions that need answering. Also Earth Shattering News...Doc Tweets. Visit Schel Reaux's myspace page and tell him we sent you. Call the comment line at (206) 309-7308.Check out insignificast.com...Thanks for listening.


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This prestigious contemporary home was designed by prominent Brisbane architect, John Walsh with beautifully landscaped gardens conveniently positioned in one of Ascot's premier streets. Within walking distance to Ascot State School and Oriel and Ascot Parks which offers the ultimate exclusive, private and low maintenance lifestyle.
From the inviting entry foyer, dominated by high ceilings throughout, the expansive open floorplan includes considerable living and dining areas with adjoining kitchen each with easy access to the pool and outdoor entertaining area. An impressive study/home office, Guest bedroom wing , luxurious bathroom and laundry are also key features of the ground floor, together with quality fixtures & fittings, including plantation shutters and Tasmanian oak floors.
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The property has an incredible spacious feel, with it's high ceilings and many large glass windows and doors to allow plenty of natural light to flow in.
Readers also enjoyed our story on Beachfront Jewel.


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Deep Root Center hosts many volunteers throughout the academic year who share their interests with our student members by mentoring our youth, becoming reading buddies, assisting with hands-on projects, and helping with behind the scenes essentials. By donating their time and knowledge, volunteers greatly benefit our members and staff, helping us realize our principles of community-based education.
Volunteering at Deep Root Center in the Age of COVID-19
Deep Root Center will be open September 2020 with modifications and a safety plan in place. As part of that Safety Plan, all of our volunteers will be engaging with our students virtually via a video conferencing platform or app. We are suggesting that SLU - CBL students use the Discord App to connect with Deep Root Center students. Other volunteers from the community may use Google Duo or Zoom. * Deep Root Center staff will review your application and contact you via email to schedule a time to talk. During that conversation we will discuss what you are interested in sharing with our students and the amount of time you are available to spend each week. Based on that info, the staff will then pair you with the student(s) who is a good match. A DRC staff person will sit in on the first conversation when you meet the student to help facilitate a schedule that will work for both of you. After that, you will meet with the young person virtually on their own.
Thank you for considering sharing your talents with the DRC community.


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If you have information about this name, share it in the comments area below!
Numerology information Filise:
Name Number: 6 Meaning: Home, Family, Love, Balance, Harmony, Help, Warmth, Social justice
Songs about Filise:
Diamanto Pios Se Filise by Vasilios Saleas from the Album Vasilios Saleas
Diamanto Poios Se Filise,Beno Mes T'Abeli - Diamanto Who Kissed you,I'm Entering The Vineyards by Andreas Andriopoulos from the Album Greek Traditional Music Collection - 18 Kalamatiana Dances
Diamantwm poiow se filise by Giannis Konstantinou from the Album Dimotika horeftika tsamika sirta kalamatiana, No. 3
Eftaiksa simpathise me/ Diamanto poios se filise by Filio Pirgaki from the Album Mia vradia sto panigiri A
Eskoteiniasan ta vouna/ Ekso vrexei kai xionizei/ Eftaikse simpathise me/ Diamanto poios se filise by Kostas Skafidas from the Album 40 Xronia Kostas Skafidas
Filise Me by Margiola Savveria from the Album Proti Praxi
Filise Me by Tzeni Haritou from the Album 25 Female Voices (78 Rmp Recordings 1946-1959)
Books about Filise:
otan vgainoun ta liontaria, filise me by margaritis kyriakos / μαργαρίτης κυριάκος (2006)
enas kakos aggelos me filise sto stithos by graikou ioanna / γραικού ιωάννα (2006)
Wiki information Filise:
Taufa'ao Filise is a Tongan rugby union footballer. A powerful prop who has played his club rugby with Bay of Plenty alongside Tonga skipper Aleki Lutui. Filise played for the Ikale Tahi in their 2001 Epson Cup campaign and on the tour to Scotland...
Drums of the Desert is a 1940 adventure film written by Dorothy Davenport and George Waggner and directed by George Waggner.


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Swirling Flourish Rubber Stamp
- Brand: Stampabilities
Enrich your creations with decorative trim using Swirling Flourish Rubber Stamp! This rubber stamp is attached to a wood block and features a curly filigree accent. Simply press into ink and apply to your crafts for a unique touch of color and design!
Wood Block:
- Length: 1 1/2"
- Width: 2 1/8"
- Thickness: 1"
- Length: 1 3/16"
- Width: 1 13/16"
Item not available in Hobby Lobby Stores.
Check your local store for availability.
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Impact Wrestling announced Kylie Rae will be at Impact All Glory on October 19 in Merrionette Park, Illinois. An opponent is yet to be announced.
The event will stream on Impact's Twitch channel and feature wrestlers from promotions like Ohio Valley Wrestling, Black Label Pro, Warrior Wrestling, Pro Wrestling Revolver, and others.
In early September, AEW released Rae, who had been away from pro wrestling months prior. During that time her Twitter was also deleted. It was reported that she was out for a medical issue.
Later that month, Rae made a surprise return to the ring at Freelance Wrestling / Freelance Underground's Game of Genders, taking on and defeating Isaias Velasquez.
BREAKING: #KylieRae is coming to #ALLGlory on @Twitch!— IMPACT (@IMPACTWRESTLING) October 7, 2019
?? https://t.co/ghA9BUWrCU pic.twitter.com/rzy58Er5mx
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Colts' Curtis Painter to make 1st career start
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With Kerry Collins still recovering from concussion-like symptoms sustained Sunday against Pittsburgh, Curtis Painter will make his first NFL start at quarterback for the Colts at Tampa Bay on Monday night.
Collins, whose status is uncertain for Monday, started Indianapolis' first three games because Peyton Manning is out until at least December with a neck injury.
Painter has mostly struggled in three years with the team.
As a rookie, he famously stepped in for Manning to try to preserve Indy's unbeaten season. The 14-0 Colts pulled their starters in the third quarter against the Jets, who rallied to win as the Colts netted one first down and committed two turnovers in six possessions with Painter at quarterback.
The next week, Painter completed 4 of 17 passes for 39 yards in a 30-7 loss at Buffalo.
Finally on TV: The Buccaneers' streak of 14 TV blackouts of preseason and regular-season games will end when they host their first Monday night game in eight years.
The Bucs said Raymond James Stadium is sold out. NFL games are blacked out in the home team's television market unless they are declared a sellout at least 72 hours before kickoff.
Don't mimic: The NFL reminded teams of the rule prohibiting defensive players from mimicking the offense's signal-calling cadence after Dallas accused the Redskins of causing the Cowboys to bungle several snaps.
As part of a memo sent to clubs, the league reiterated that it was unsportsmanlike conduct for defenders to use "acts or words" that were "designed to disconcert an offensive team at the snap."
New Dallas center Phil Costa misfired on four snaps in Monday's win, and afterward the Cowboys accused Washington of mimicking Tony Romo's cadence, which the Redskins denied.
Briefly: Cowboys receiver Dez Bryant (thigh) is questionable against the Lions after missing a second straight day of practice. Quarterback Tony Romo (cracked rib) was limited in practice. He said he expects to play. ... The Packers say running back Ryan Grant and right tackle Bryan Bulaga won't play against the Broncos. ... Jets center Nick Mangold (ankle) practiced for the first time in two weeks.


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- Unisex sunglasses
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A youngster who suffered severe head injuries in an incident in Coventry has amazed doctors and is now on the mend.
The family of 17-year-old Stephen Thomson were told to expect the worst after he was hurt in an incident in Broadgate, Coventry city centre, on Saturday, July 17.
He spent several weeks in intensive care at Walsgrave Hospital and was so poorly his family had him Christened while he was on a life support machine.
They released a picture of the stricken youngster in a bid to prick people's consciences and prompt them to contact police with information about the incident.
But now the former pupil of Alderman Callow School in Mitchell Avenue, Canley, Coventry, is conscious and able to speak.
DC Sherrie Barrett, of Little Park Street police, said the youngster was in the rehabilitation unit at Walsgrave Hospital.
She said: "Doctors are saying it's a miracle and he's likely to make a substantial recovery."
She said Stephen's recovery had come as a huge relief to his family, including mum Jacqueline Thomson, 46, Stephen's dad, also called Stephen, 47, twin sister, Sarah, and brothers, Marc, 26, and Daniel, 20.
She said: "The family are over the moon."
DC Barrett said Stephen, who is known to his friends as Floyd, could soon move to a specialist centre in Leamington for intensive treatment to speed his recovery.
She added: "It's a long road, but at least he's on the start of it."
* Two men have been charged with affray and assault in connection with the incident.


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You may directly drag and drop video link from your browser to the drop box to start a download, you don't even need to copy and paste video link manually. The higher the quality the bigger the file, so the trick is to balance quality and size: unless you're watching on a really big screen, you can probably manage without p quality unless you've got oodles of spare storage although p video won't look good on an HD display.
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This practice is useful for ensuring quality in any Collaborative translation initiative, but it is particularly relevant where you are tapping into a large crowd of unknown contributors.
Problem description
Ensuring quality in Collaborative translation can be a challenge. One way to approach this issue is to vet contributors, instead of individual contributions (ex: Entry Exam).
One danger with this approach is that a given contributor could start producing low quality content once s/he has been vetted. For example, if the bar for the Entry Exam is low, a spammer could invest a bit of time to pass it, and get entry into the system.
Therefore, complement a-priori vetting of contributors, with occasional, random spot checks on the quality of their contributions.
Links to related patterns
- This practice complements Entry Exam.
Real-life examples
As of November 2011, the World-Wide Lexicon project is working on an approach that will include random spot-checking.


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Please help me with the answer and the solution
Dear Student, Sand does not dissolves in water. In a mixture containing sand and water, the separation method that can be used is decantation. The sand will settle at the bottom and water can be decanted. The wet sand can then be left for evaporation and hence, dry sand remains. Regards


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Sagittarius Daily Horoscope Today Saturday 15th February 2020
Sagittarius, don't mortify yourself by spending the day thinking about everything you've done wrong these days. Leave the past behind and focus on the day you are in, many times we let wonderful things happen by not knowing how to appreciate our present and focus on things that are not worth it, s early hours today you are likely to receive some important news about of a loved one or a relative, try to remain calm and think well what you will do, it is never good to be impulsive.
In another area, good news will likely arrive throughout the week, remember that calm always comes after the storm.
For today, if you are single, the stars have a big surprise for you, as many encounters, leagues and the possibility of going out with many people of the opposite sign await you. This does not mean that you are going to find a definitive partner, but you will have a good time.
The efforts you make daily to keep everything going well in your relationship pay off. The astral atmosphere, especially the presence of the Moon in your sky, allows you to take the necessary initiatives to make your love life healthy and peaceful. If you are single, you like your loneliness right now. You have found your rhythm and do not want to invest yourself 100% in a relationship that will not get you very far. "To know how to wait there is everything to gain".
You are close to finding that person that will complement you in everything if you still do not get it, and if it is already with you then you have to put everything on your part to make it work, do not fail that person because it is one of the best options for you
Give yourself today to rest from that stress that you carry with you throughout the week, you must take care of your state of inner peace since the stars today are not in your favor. But don't worry, this is only temporary. Better days are coming.
Money and Luck
Money has never been a problem for you and your family, remember that life is like a roulette wheel. For what you must learn to save, avoid any waste of unnecessary money. Try to help your neighbor, because as they need right now, you can find yourself in the same situation later. Sagittarius Luck Today
It is a good time to start a family business, will give good results and in what corresponds to your economic stability will not be affected by this expense you will make. Do not limit yourself to investing in this business since it will grow over time and leave you more than what you invested in it.
You are in a sad mood. Like Bartleby, "you would rather not work". Melancholy embraces you, you have all the trouble in the world to change things. You feel alone, excluded, unsuitable. Rest assured your professional entourage wants you good. You are deeply appreciated and the expressions of sympathy will not fail to arrive. Take hold of your outstretched hands (starting pot, lunches, integration weekend, etc.) and you will quickly get back on track.
Family and Friends
Your friends multiply attractive invitations. Naturally curious, nothing stops you: from sporting escapades to disguised dinners, to afternoon board games. Life is so good when you are so well supported! At home, doubt settles. When you try to get closer to your family members, they run away from you! Nothing serious, your loved ones just need a little space. Once the crisis is over, your complicity will return as before.
You need to rest more, it is evident that, due to your rhythm of life, your body does not rest enough to continue with your day to day, that is why you should take into account resting more. This will be great for your brain and will make you more awake and happy.
You display an impressive serenity. Your benefactress Venus brings you calm and makes you feel harmony. Ascendant Sagittarius, you redouble your enthusiasm to accomplish your projects. You have skillfully trained your body to love what makes it feel good. You take care of yourself, this is noticeable at first glance. You who can sometimes be sensitive to the bronchi have found a new breath. What is your secret?
- Sagittarius Daily Horoscope of February 14th, 2020
- Sagittarius Daily Horoscope of February 15th, 2020
- Sagittarius Daily Horoscope of February 16th, 2020
- Sagittarius Weekly Horoscope February 10th to 16th, 2020
- Sagittarius Monthly Horoscope of February 2020
- Sagittarius in 2020| Full Year Horoscope


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September 13, 2019
Trying out for a new ice hockey team can be an extremely nerve-racking experience! For many players, tryouts are more difficult than actual games. Most of the apprehension surrounding ice hockey tryouts is based on a lack of preparation, though. Whether you're trying o...
December 8, 2016
Great article from http://hockeyfamilyadvisor.com/2016/05/09/
8 Things Coaches Look for When Selecting their Team
By taking the time to prepare and maintaining a good attitude, you can show the coaches what an ideal fit you make to a successful team
For some players, tryo...
October 4, 2016
James Leath
By James Leath
I have coached youth sports since I was in high school. I am now in my mid-thirties. If there is one thing that has not changed, it is the child's dread of the car ride home.
One thing almost every athlete had in common was how much they looked forward to...
September 10, 2016
July 26, 2016
Self Confidence is Hard Work
One of the main messages from "The Kings Confidence" is that George VI became confident at public speaking through hard work.
In my post "Are affirmations useless?" I discussed one of the founders of personal development, Samuel Smiles....
July 15, 2016
07/15/2016, 2:15pm MDT
By USA Hockey
July 15, 2016 | It's NHL Development Camp season and all across North America, teams are utilizing cross-ice hockey, small-ice hockey and small-area games to teach their prospects.
"To me, the (small-ice) 3-on-3 is more important than...
April 25, 2016
Great article below from Follow this link to the article
Before my rookie year in the NHL, my dad made me a Wayne Gretzky highlight tape to study. I have no idea how he did it. This was 1985. We didn't have computers. When you wanted to m...
June 27, 2014
Jeremy Trevors - Daily Gleaner
The Daily Gleaner (Fredericton)
Fri Jun 27 2014
Page: B1
Section: Sports
Byline: Jeremy Trevors The Daily Gleaner
It's a big weekend for a pair of Frederictonians going into the 2014 National Hockey League Entry Draft in Philadelphia, Pa.
Defenceman Matt Murphy of the Ha...
May 8, 2014
Bruce Hallihan - Daily Gleaner
Dax MacLean and Stacy Smallman have a lot in common.
They both helped the University of New Brunswick Varsity Reds men's hockey team win national titles – sniper MacLean with a nose for the net for Mike Kelly's 1998 squad, scrappy Smallman with a nose for the corners fo...
April 25, 2014
Champions Hockey
Hockey Family Advisor is a great website with tips for hockey players of all skill levels and aspirations, as well as their families. They recently posted an article on building nutrition strategies. This particular article is focused on older players looking to add mu...
Tips for Ice Hockey Team Tryouts
The Art of the Post Game Conversation
Top 10 Signs You're a Great Teammate!
Hard Work = Self Confidence
Cross-Ice Hockey Prominent at NHL Development Camps
Adam Oates - Skills Development
CH Alumni projected to go in NHL draft
Champions Hockey: Award Winning Formula
Muscle building nutrition strategies for Hockey Players
September 2019
December 2016
October 2016
September 2016
July 2016
April 2016
June 2014
May 2014
April 2014
March 2014
Skills & Development
Prep Camps (Fred)
Prep Camps (Miramichi)
Novice AAA Winter


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As a resale marketplace, prices may be above face value.
It looks like there are currently no tickets available for this event.
Try the options below to find what you're looking for.
Find tickets for House Of Blues Gospel Brunch in Dallas, TX at House Of Blues - Dallas on November 22, 2020. House Of Blues - Dallas is located in Dallas, TX


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The Infinite Mantra is a yoga inspired family board, remarkably stable to free your mind from worry and give you courage to breathe in the fresh air of adventure.
Glide easily across any calm water on a spacious, unhindered deck pad, which is always perfect for furry friends or your shavasana.
Hand crafted with premium materials and stocked with the highest grade accessories, this complete package is ready for adventure when you are.
Enjoy peace of mind with SeaGods lifetime warranty.
Best rated Inflatable Paddle board for Family & Yoga
- 11' x 34" x 6"
- Yoga/ Multiple riders
- Flatwater Lakes/Ocean
- Strong and Light
- Easy to Ride
- Extra Handles
- Shoulder Carry Strap
- Complete Accessory Package
- Bonus action camera mount
Design Highlights - Also Includes:
- Latest PVC fusion technology and heat laminated seams
- Exclusive Apollo UV protective spray glazing
- Strongest PSI range for ultimate rigidity and additional weight support
- Highest caliber bungee cargo tie down area and bonus action camera mount
- Light to carry and quick to inflate
- Premium materials for surpassed military grade durability
iSUP/SUP Accessories - Included:
- Complete Accessory Package
- Carbon fiber paddle
- Wheeled hiking backpack
- Double action high pressure pump
- US fin box with no-tool fin
- FREE 11' coil SUP leash
Construction & Design Highlights
- Dimensions
- Length – 11 foot 0 inches
- Width – 34 inches
- Depth – 6 inches
- Weight – 28lbs
- Volume – 280L
- Rider Weight – 350lbs
The Infinite Mantra 11 Design Full Specs:
- Super reinforced 1000 Denier Nylon Drop Stitch Fabric
- Durable, Rigid, Rated above 20PSI
Reinforcement Layer
- DWF Fusion layer to seal drop stitch fabric
- Holds board shape
- Creates high strength air tight body
- Machine Laminated directly to drop stitch
Final layer
- Military grade 1000D PVC
- 7mm thickness rated for 25 PSI
- Durable, rigid, impact resistant
- Machine laminated to DWF layer
- FIVE layer Military PVC
- Heat Laminated seams throughout
Deck Pad
- Oversize Pad
- UV Printed high traction EVA Graphic pad
- 5mm thickness and 55A rigidity
iSUP Accessories
- Front 6-point tiedown with bungee
- Rear tie down d-ring
- Anchor tie down on front bottom
- Shoulder carry strap
- 29PSI rated Scoprega Inflation Valve
- Front/Center/Rear Integrated handle
- Offset center handle for smooth yoga surface
- Wheelie Hiking Backpack carry bag
- 3-piece Carbon fiber paddle
- Coil Leash
- No tool 9-inch plastic fin with Thumb screw
- Scoprega Dual Action Inflation/Deflation pump
If you have any questions at all, please feel free to contact us anytime as we would be happy to help!
Our Blogs
Learn with us
At Sea Gods, we want your board to inspire you but we also want to make sure you are prepared for all elements of your adventure!


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NWS: Tornado Watch for San Angelo and West Texas
SAN ANGELO, TX – The Storm Prediction Center in Norman, OK has issued a tornado watch for San Angelo and most of west central Texas until midnight Wednesday night.
A tornado watch means weather conditions could allow tornadoes to form within any severe thunderstorm.
Severe thunderstorms are possible this evening through early Thursday morning.
The main hazards will be large hail, damaging winds, and a few tornadoes.
The forecast calls for a chance of showers and thunderstorms, then showers and possibly a thunderstorm after 8 p.m. Some of the storms could be severe.
Low around 60. South wind 5 to 15 mph. Chance of precipitation is 80%.
New rainfall amounts between a tenth and quarter of an inch, except higher amounts possible in thunderstorms.
This is a developing situation.
Get more stories like this by signing up for our daily newsletter, The LIVE! Daily.
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Millions To Be Made, Billions To Be Sent-And At Wholesale!
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I have, in just the past year alone, won the British National Lottery twice, inherited enormous sums of money from relatives at least two forests removed from my own family tree, and, like many of you, been the fortunate recipient of the generosity of an African prince/government official who just needs a small downpayment in order to share with me the millions of dollars/Euros that await me.
That's the good news. The bad is that I'm apparently lacking as a lover, going to outlive my money and die lonely and cold, and don't know anything about what's going on with all the talking behind my back.
Twenty years ago I never would have known any of this. Today, I do, thanks to spam. Come to think of it, if I had shared with anyone 20 years ago that all my knowledge was due to SPAM there really would have been more people talking about me (and my mental capacity) behind my back.
If the Internet is the World of Information then spam is its seedy neighborhood. If the products and services offered by solutions providers to credit unions that are reported in Credit Union Journal and elsewhere are the cutting edge, spam remains the dull background.
Due to some e-mail related issues recently, I was forced to turn off my spam filter for a while. Management consultants sometimes refer to too much data as "drinking from the firehose." Turn off your spam filter sometime and you'll be drowning in a perpetual geyser of, well, consider some of these e-mail subject lines: "An idealist has died" (Then I'm thinking he didn't see that one coming); "Everyone will notice the difference in you" (no mention if that would be a positive or negative); "Lots to love-wholesale prices!" (Hey, what's better than lots of love? Not overpaying for it!); "Business Intuition-Learn How to Find Yours" (How, incidentally, does one "learn" intuition?); and "Top watches. Big sale. Hurry up" (How will I know if I'm hurrying if I don't have a watch?).
What you may not know is that the Internet did not "create" spam. It has been around for centuries. Before bulk snail mail, one account of the history of spam notes that in the late 19th century Western Union allowed telegraphic messages to be sent to multiple destinations. "The first recorded instance of mass unsolicited commercial telegram is from May 1864," the account reported.
Still, I'm willing to wager that Civil War-era telegraph messages never included any of these subject lines: "Make Every Meeting Count- with a Little Help from a Friend" (a message I nearly clicked on); "Do you know what chicks like?" (If I did, would I be editing Credit Union Journal?); "This is about the very fundamental survival of our species!" (I'm going to feel bad having deleted this if it turns out to be true), and "You can help consolidate Scotland's debt (That's odd, as I thought the Scots never spent any money).
And now for something completely different. That junk e-mail has come to be widely called "spam" has created an interesting dilemma for the folks at Hormel, the maker of the luncheon meat. It's pursued some litigation against companies manufacturing software with "Spam" in the name and seen the cases dismissed, winning only on its point that any references to its product be spelled "SPAM." It's generally believed that "SPAM," of all things, became "spam" as the result of a 1970 Monty Python Flying Circus sketch in which every item on the menu at a café includes SPAM in it, and a group of Viking patrons drown out all conversations with a song that repeats "SPAM, SPAM SPAM..." (If you want to see the sketch or the reasons why the British would find SPAM funny and relevant, you can find it, appropriately, online).
In the early days of the Internet when only geeks populated nascent bulletin boards, some would type in "SPAM" repeatedly to push others' postings off of the screen. Others would fill the screen with quotes from the Python sketch. Speaking of geeks, Wikipedia notes that rival fans of Star Trek and Star Wars would often try to spam the others' message boards (talk about two groups that could use love at wholesale prices).
I doubt even the genius of the Monty Python troupe could have envisioned some of the subject lines that appeared in spam e-mail I received. Consider: "Vegetable shoes" (for the true vegetarian); "We've got meds" (not unusual, but the sender was Lobsterdirect.com), and "Your pants are on fire and ice!" (I guess that's why they have two legs).
According to a one history of "spam," the earliest documented spam was sent in 1978 by Digital Equipment Corp. to 393 recipients. The first electronic chain letter made its dubious debut in 1988, and the first commercial spam was sent by two lawyers (and why not when you don't have to worry about lowering folks' perceptions of you) in 1994.
Thank goodness. Otherwise, I never would have received an offer that included just one long string of question marks, or a recent opportunity to buy a "Complete replica of the Philippines," or a favorite of mine, "An e-mail to you from C.S. Lewis." Mr. Lewis died in 1963, so the fact he's still sending e-mail is impressive. Fittingly, among Mr. Lewis' works was "The Screwtape Letters." He had no idea.
Frank J. Diekmann can be reached at fdiekmann


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Color and Cocktails Design Panel
Come to the Ferguson space at CAMP for a complimentary cocktail and an interactive panel discussion with representatives from Signature Hardware, Dunn-Edwards, Nicholas Lawrence Design and Palm Springs Life magazine which will explore the fascinating world of color trends.
Panel: Lisa-Marie Hart, Editor of HOME+DESIGN Palm Springs Life, Sean Fisher, Director of Brand Experience, Signature Hardware, Sara McLean, Color Expert and Stylist, Dunn Edwards, Nicholas Hertneck & Larry Lazzaro, Nicholas Lawrence Design.
Free, Non-ticketed
Things to Know
This event is for all ages.
The organizer of this event is Ferguson.
Event Check-in Location
Modernism Week CAMP, 575 N Palm Canyon Drive, Palm Springs, CA 92262
Photo Credits: Ferguson
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Reports and resources
The International Rescue Committee uses our learning and experience to assist people affected by crisis and shape humanitarian policy and practice. Browse our research and resources.
IRC needs assessment report: Mexico-northern border
A new IRC survey from the Mexico-U.S. border finds needs are overwhelming available services. Women and children are at risk.
David Miliband in The Guardian: The most vulnerable pay for Trump's 'manufactured crisis'
IRC chief says U.S. government 'failing in its most basic responsibilities' in its handling of border issues
Disorder by design: a manufactured U.S. emergency and the real crisis in Central America
This IRC report reveals the manufactured tensions created by the Trump administration at the U.S.-Mexico border and the real crisis in Central America, and recommends solutions.
The IRC's top research findings from 2018 and 2019
This document highlights the International Rescue Committee's top insights from 2018 and those to watch out for in 2019.
The IRC's research: looking back on 2018, looking forward in 2019
This document summarizes research findings and ongoing research across all IRC outcomes throughout 2018 and 2019.
Client Responsiveness Measurement Framework
The Client Responsiveness Measurement Framework aims to help organisations monitor, assess and verify how well they are "putting people at the centre" by using inputs and feedback from affected people in decision-making around aid programmes.
A Guide for Client Responsive Staff Management
The purpose of A Guide for Client Responsive Staff Management is to provide guidance and resources to staff of humanitarian agencies on the competencies required to develop a client responsive orientation.
Everything on her shoulders: rapid assessment on gender and violence against women and girls in the Ebola outbreak in Beni, DRC
In December 2018, the International Rescue Committee (IRC) completed an assessment in Beni, DRC, with the aim of examining the effects of the EVD outbreak and response on women and girls, and specifically how the outbreak has impacted women and girls' experience of violence, also considering the pre-existing context of insecurity in which they live.
IRC U.S. Refugee and Asylum Policy Newsletter – February 2019
Highlights, data, helpful resources and the IRC's response to current U.S. refugee and asylum issues from the IRC's Global Policy and Advocacy team.
Refugee resettlement by the numbers: Admissions plummet in FY18
Nationwide refugee admissions dropped precipitously in Fiscal Year 2018 (FY18) compared to the year prior and historic trends.


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Softmax is a commonly used function for turning an unnormalized log probability into a normalized probability (or categorical distribution).
Say is the output of a neural network before softmax, we call the unnormalized log probability.
After softmax, we usually sample from this categorical distribution, or taking an argmax function to select the index. However, one can notice that neither the sampling nor the argmax is differientiable.
Researchers have proposed several works to make this possible. I am going to discuss them here.
I will introduce Gumbel Softmax [1611.01144], which have made the sampling procedure differentiable.
First, we need to introduce Gumbel Max. In short, Gumbel Max is a trick to use gumbel distribution to sample a categorical distribution.
Say we want to sample from a categorical distribution . The usual way of doing this is using to separate into intervals, sampling from a uniform distribution , and see where it locates.
The Gumbel Max trick provides an alternative way of doing this. It use Reparameterization Trick to avoid the stochastic node during backpropagation.
where , which can be sampled by . We can prove that is distributed according to .
, where which can be sampled by is distributed with
Gumbel Distribution (param by location , and scale ) (wikipedia) CDF: PDF: Mean: is the Euler–Mascheroni constant. Quantile Function: (Quantile Function is used to sample random variables from a distribution given CDF, it is also called inverse CDF)
We actually want to prove that is distributed with .
We can find that has the following PDF and CDF
.Then, the probability that all other are less than is:
We know the marginal distribution over and we are able to integrate it out to find the overall probability: ()
which is exactly a softmax probablity. QED.
Reference: https://lips.cs.princeton.edu/the-gumbel-max-trick-for-discrete-distributions/
Notice that there is still an argmax in Gumbel Max, which still makes it indifferentiable. Therefore, we use a softmax function to approximate this argmax procedure.
where is a temparature hyperparameter.
We note that the output of Gumbel Softmax function here is a vector which sum to 1, which somewhat looks like a one-hot vector (but it's not). So by far, this does not actually replace the argmax function.
To actually get a pure one-hot vector, we need to use a Straight-Through (ST) Gumbel Trick. Let's directly see an implementation of Gumbel Softmax in PyTorch (We use the hard mode, soft mode does not get a pure one-hot vector).
def gumbel_softmax(logits, tau=1, hard=False, eps=1e-10, dim=-1):# type: (Tensor, float, bool, float, int) -> Tensorr"""Samples from the Gumbel-Softmax distribution (`Link 1`_ `Link 2`_) and optionally discretizes.Args:logits: `[..., num_features]` unnormalized log probabilitiestau: non-negative scalar temperaturehard: if ``True``, the returned samples will be discretized as one-hot vectors,but will be differentiated as if it is the soft sample in autograddim (int): A dimension along which softmax will be computed. Default: -1.Returns:Sampled tensor of same shape as `logits` from the Gumbel-Softmax distribution.If ``hard=True``, the returned samples will be one-hot, otherwise they willbe probability distributions that sum to 1 across `dim`... note::This function is here for legacy reasons, may be removed from nn.Functional in the future... note::The main trick for `hard` is to do `y_hard - y_soft.detach() + y_soft`It achieves two things:- makes the output value exactly one-hot(since we add then subtract y_soft value)- makes the gradient equal to y_soft gradient(since we strip all other gradients)Examples::>>> logits = torch.randn(20, 32)>>> # Sample soft categorical using reparametrization trick:>>> F.gumbel_softmax(logits, tau=1, hard=False)>>> # Sample hard categorical using "Straight-through" trick:>>> F.gumbel_softmax(logits, tau=1, hard=True).. _Link 1:https://arxiv.org/abs/1611.00712.. _Link 2:https://arxiv.org/abs/1611.01144"""if eps != 1e-10:warnings.warn("`eps` parameter is deprecated and has no effect.")gumbels = -torch.empty_like(logits).exponential_().log() # ~Gumbel(0,1)gumbels = (logits + gumbels) / tau # ~Gumbel(logits,tau)y_soft = gumbels.softmax(dim)if hard:# Straight through.index = y_soft.max(dim, keepdim=True)[1]y_hard = torch.zeros_like(logits).scatter_(dim, index, 1.0)ret = y_hard - y_soft.detach() + y_softelse:# Reparametrization trick.ret = y_softreturn ret
When fowarding, the code use an argmax to get an actual one-hot vector.
And it uses
ret = y_hard - y_soft.detach() + y_soft,
y_hard has no grad, and by minusing
y_soft.detach() and adding
y_soft, it achieves a grad from
y_soft without modifying the forwarding value.
So eventually, we are able to get a pure one-hot vector in forward pass, and a grad when back propagating, which makes the sampling procedure differientiable.
Finally, let's look at how affects the sampling procedure. The below image shows the sampling distribution (which is also called the Concrete Distribution [1611.00712]) and one random sample instance when using different hyperparameter .
when , the softmax becomes an argmax and the Gumbel-Softmax distribution becomes the categorical distribution. During training, we let to allow gradients past the sample, then gradually anneal the temperature (but not completely to 0, as the gradients would blow up).
from Eric Jang. https://blog.evjang.com/2016/11/tutorial-categorical-variational.html
How to make argmax differentiable?
Intuitively, the Straight-Through Trick is also applicable for softmax+argmax (or softargmax + argmax). I am still not sure, needs more digging in the literature.
Some have introduced the soft-argmax function. It doesn't actually makes it differentiable, but use a continuous function to approximate the softmax+argmax procedure.
where can be a large value to make very much "look like" a one-hot vector.
softmax + argmax is used for classification, we only want the index with the highest probability.
gumbel softmax + argmax is used for sampling, we may want to sample an index not with the highest probability.
softmax + argmax is deterministic. Get the index with the highest probablity.
gumbel softmax + argmax is stochastic. We need to sample from a gumbel distribution in the beginning.
Output vector
softmax and gumbel softmax aboth output a vector sum to 1.
softmax outputs a normalized probability distribution.
gumbel softmax outputs a sample somewhat more similar to a one-hot vector.(can be controlled by )
Straight-Through Trick can actually be applied to both softmax + argmax and gumbel softmax + argmax, which can make both of them differentiable. (?)
Gumbel Softmax [1611.01144]
Concrete Distribution (Gumbel Softmax Distribution) [1611.00712]
Eric Jang official blog: https://blog.evjang.com/2016/11/tutorial-categorical-variational.html
PyTorch Implementation of Gumbel Softmax: https://pytorch.org/docs/stable/nn.functional.html#torch.nn.functional.gumbel_softmax


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This function uses multiple metrics to collect data in the last 30 days, including the traffic and bandwidth, storage, and transcoded video length. This function also allows you to download detailed data.
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The BE-GOOD challenge of Hoogheemraadschap (the regional water board in the southwest Netherlands) focusses on gaining more insight into the water quality and quantity, as well as the potential impact of its sewer systems on the surface water. For this, Hoogheemraadschap Delfland contracted the SME HydroLogic to develop the SWWIM (Smart Wastewater Information Management) platform using HydroNet. The platform is currently in the development phase and includes two municipalities within the Delfland region.
How the idea behind HydroNet inspires VMM
HydroNet is a web-based decision support system which transfers weather and water data into sophisticated applications and dashboards. This system is already used by multiple water boards in the Netherland, such as Waterschap Brabantse Delta and Waterschap De Dommel. Representatives of those waterboards and HydroLogic met with Flanders Environment Agency (VMM) and Hoogheemraadschap Delfland to exchange their ideas and experiences using HydroNet in making well-informed and transparent decisions for the analysis and sustainable management of water resources.
These experiences help VMM to develop their own decision making tool, possibly based on HydroNet, for water management in the future. Once again, transnational knowledge transfer inspires multiple BE-GOOD partners.


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Retirement Plans + Compliance Woodland Hills, CA
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This retirement plan option allows employers to set up accounts for both their employees and themselves. SEP IRAs are popular because they are easy to set-up, and employers are only required to make contributions at a fixed percentage for every eligible employee. Additionally, employers gain a bit of flexibility with these plans since they can decide the maximum amount they want to contribute each year.
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These retirement plans are famous for allowing small businesses, as well as employees, to contribute a percentage of their income to retirement by investing pre-tax money. Sometimes, employers who administer 401(k) plans will decide to match the contributions made by their employees. Also, 401(k) plans tend to have higher contribution limits, which helps employees maximize their retirement savings and funds.
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This retirement plan was created for employers that experience fluctuations in profits each fiscal year, letting them choose their own contributions. Profit-sharing plans allows clients to have access to plan loans, vesting schedules, service requirements, as well as a level of flexibility not offered by SEP plans.
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At The Ryding Company, our mission is to ensure that every retirement plan and fund is successful for both employers and employees. The retirement planning industry is filled with jargon, rules, and regulations that can give any plan administrator a headache. That's where we come in. Given our experience, expertise, and resources, The Ryding Company handles paperwork and compliance so your retirement plan can function effortlessly. Here are just some of the resources you gain access to as our valued client:
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The bargaining committees for the City of Toronto and TCEU Local 416- CUPE have been in collective bargaining for more than three months with the aim of achieving a renewed collective agreement. To date, the parties have reached agreement on a number of items.
As is the norm in the course of bargaining, late last month the City requested that the Province appoint a conciliation officer to assist with negotiations. The City looks forward to working with the conciliation officer to achieve a negotiated collective agreement with Local 416.
Collective bargaining with CUPE Local 79 remains ongoing.
The City's objective is to negotiate a collective agreement that builds on previous rounds of bargaining, an agreement that is fair and reflects the important role City employees play in Toronto, and one that is affordable for the city's residents and businesses.
The collective agreements for both Local 416 and Local 79 expired on December 31, 2019.


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Dentistry for Children & Adults Too!
Bay Area Kids Dentist-General Dentistry for Adults-Dental Office
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Happy to volunteer for this year's annual trash and litter cleanup up in San Jose hosted by "Alex Saves the Planet", a very worthy cause. Dr. Cho and staff love helping clean up our neighborhoods and hope you do too. Doing our part to help make thi...
April 15, 2019
Dr. Benjamin Cho
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The most frequent question that parents ask is when their baby's first dental check-up should occur. Most parents are surprised to learn the first visit is much earlier than they thought. The American Academy of Pediatric Dentistry recommends the fir...
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Cancellation Announcement of 2020 WCA Elite Competition
WCA Elite Competition has been held in January, February each year. But this year Competition has been cancelled due to the latest situation of the novel coronavirus epidemic and school suspension. The safety and health of the participants and the staff always come first in our consideration.
Thank you for your attention and support to this Competition. We are sorry to the inconvenience caused as a result of its cancellation. We wish everyone good health and have the opportunity to see you in the future.
Frequently Asked Questions:
Q: Now 2020 WCA Elite Competition is cancelled, can 2019 Double Distinction students do the 2021 WCA Elite Competition instead?
A: By the competition rules, current year WCA Elite Competition only invites WCT Double Distinction students from last year. Due to this year special circumstances, 2019 Double Distinction students can apply for doing the 2021 WCA Elite Competition. We shall announce the details prior to 2021 Elite Competition application. By then, parents will be contacted via email/SMS.
Q: In 2019, the student took the Age 8-11 group WCT. By the end of 2020, the student will be 12 y.o. For 2021 WCA Elite Competition, which Age group he will be invited to join?
A: According to the competition rules, 1 Janaury 2021 is the cut-off date to decide whether a student will take Age 8-11 or 12-14 group in the 2021 WCA Elite Competition. In order words, a student will be invited to do the Age 8-11 Competition if he is still 11 y.o. at the end of 2020. If a student is already 12 y.o. on 1 January 2021, he will be invited for the Age 12-14 group at the Competition.
Enquiries: 2354 0890


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