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On Monday, September 16, 2013, Village of Palatine Mayor Jim Schwantz and Palatine Emergency Management Agency (EMA) Coordinator Tom Smith accepted the StormReady certification from Jim Allsopp, Warning Coordination Meteorologist for the National Weather Service (NWS).
StormReady is a voluntary program which helps communities better prepare for and mitigate effects of extreme weather-related events. StormReady also helps establish a commitment to creating an infrastructure and systems that will save lives and protect property. StormReady communities are better prepared to save lives from the onslaught of severe weather through advanced planning, education and awareness.
Left-to-right: Village of Palatine Mayor Jim Schwantz, Palatine Emergency Management Agency (EMA) Coordinator Tom Smith, and National Weather Service Warning Coordination Meteorologist Jim Allsopp.
The Palatine Emergency Management Agency (PEMA) working in conjunction with the NWS worked on meeting the standards required to achieve this certification. Requirements included advance warning to residents and staff, trained weather spotters capable of providing the NWS information during storms, providing weather spotter training to the public, creating specific plans for sheltering in place during severe weather and providing NOAA Weather Radios to schools and other locations where large number of people gather.
"Going through this process allowed us to look critically on how we prepare for and respond to extreme weather", said PEMA Coordinator Tom Smith. "Although no community can proclaim that they are 100% ready for any severe weather event, Palatine has taken steps over the years to be as prepared as reasonable should these incidents occur."
For more information about the StormReady Program or other emergency management topics, please go to www.palatine-ema.org.
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Taapsee Pannu's latest outing Naam Shabana has shown decent jump in its collection at the Indian box office on Saturday. Its 2-day gross total has crossed Rs 15 crore mark in the domestic market.
Having good hype and promotion, Naam Shabana was released in 2,100 screens across the domestic market on March 31 and it collected Rs 5.12 crore at the domestic box office on the first day. It broke the record of Pink (Rs 4.32 crore) and became the biggest opener for Taapsee Pannu. Taran Adarsh tweeted: "#NaamShabana gets a boost due to Akshay's star power... Evening shows fare better... Fri ₹ 5.12 cr. India biz."
The Shivam Nair-directed action spy-thriller film received mixed review from the viewers and critics, who said that it lacks the thrill. The trade experts from the Bollywood predicted that the negative word of mouth would take toll on its business on the following days. But to their surprise, the film showed good growth in its collection on Saturday.
Naam Shabana collected Rs 6.37 crore nett at the Indian box office on Saturday and its 2-day total collection has reached Rs 11.49 crore nett. Its gross total stands at Rs 15.96 crore. Taran Adarsh tweeted: "#NaamShabana witnessed healthy 24.41% growth on Sat... Sun biz is crucial... Fri 5.12 cr, Sat 6.37 cr. Total: ₹ 11.49 cr. India biz."
Naam Shabana, which is produced by Neeraj Pandey and Shital Bhatia, has been made with a medium budget of Rs 15 crore. Its music and satellite rights have fetched a decent sum for its makers. Its performance at the ticket counters is beyond the expectations of its producers and distributors, who are all thrilled over its superb collection. The film is said to be profitable venture in just two days.

subset: commoncrawl


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NISAmerica release an English trailer for its upcoming RPG, The Awakened Fate Ultimatum.
The trailer focuses on the games story element, which sees you having to make the choice of who is to live or die between two factions of angels & demons. The roguelike RPG is poised for a release in the west on the Playstation 3 during Spring 2015.


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We all aware of one fact about relationship i.e. Relationships aren't easy to make. It involves a lot of hard work and time to build trust. For a strong relationship, you need to believe in your partner fully. When you start having doubt on someone you love, your relationship starts breaking. If you don't trust your partner then you need to find out the reason for the same. Reason to make you believe that everything is good between you and your partner.
What is relationship counselling?
When the trust and love start fainting in a relationship then couples are advised to seek for help. To make a healthy relationship both require to built trust. A counsellor helps them to see the spark in their relationship and regain trust. Online counselling helps a couple to have deep conversations and show them their little mistakes that became so big that their relationship about to break. Relationship counselling help partners stay in strong relationships.
How does online relationship counselling work?
Online counselling or online therapy is now easy to access for those who need it to save their relationship. Online relationship counselling provide couple, the privacy of your home and gives the advantage to contact your online counsellor through video, call or chat with a good internet link, a working microphone and computer.
What are the advantages of online marriage counseling or online relationship counseling?
- Economically Fit for your Need.
As per the old saying, "time is money". Online therapy helps you save the time by providing you counselling services at your place and you don't need to travel far from your home or your place to counsellor Office. If you have children or elderly parent at home then there is no need to look for someone to take care of them because you gonna have counselling at your own place.
- More probability to you both make the appointment.
If we compare the in-person couples therapy and online couple counselling, then online therapy will win the session attendance.
Because online therapy still works if the babysitter cancels or the car breaks down. This is the big plus.
- No excuse to leave the sessions.
"I am not attending this session with you" This line often use as a wepon by a couple to one another in a conflict. Online counselling comes with the advantage that the sessions are in your own house, these kinds of excuse lose their influence. You are both at the house, you might as well do the session.
- No need To attend the session together.
You and your partner can choose a separate place or attend a session differently. Many counsellors are ok with counselling with one partner on a third line.
- Online medium provides a more open and communicative environment.
As we talk above that online counselling provide the advantage of choosing your own comfortable place and when you are in your comfort zone it's easier to be open up about your problems. There is no need to be stressed by having the thoughts to leave your place.
Hope now you get the clear idea about the Relationship Counseling and How Relationship Counseling Works? If you still looking for the more answers then you should visit BetterLYF.


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Where Has All the Good News Gone?
On New Year's Eve 2013 I was having an optimistic moment. I wrote then that I was sure that the "tide of love and compassion is turning" for the better. I sort of promised that in my daily reading of my local newspaper, the Dallas Morning News, I would find evidence to support my optimism, and I would share it with you. Well, that was almost a month ago and I have to say that so far I'm not as overwhelmed with the good news as I expected to be.
I am truly not disappointed in this lack of the uplifting, for I still think it is happening every day. What I am disappointed in is the apparent reluctance of the DMN to print any of it. But, true to my word, I will share the bits that have escaped the beady eyes of the news editors.
1. A bill both parties can live with
Congressional appropriators unveiled a $1.1 trillion spending bill negotiated by both parties that would fund the government until Sept. 30. The good news here is that congress actually agreed on something.
2. Here's something that made it into the Sunday part called "Talking Points".
"There are about 80 different species, and some of them look just like us. And they could walk down the street, and you wouldn't know if you walked past one." This is credited to former Canadian defense minister Paul Hellyer, on extraterrestrials who visit Earth. The good news is he is not made to seem like a nut job. I like Mr. Hellyer and I'm glad he made the paper.
3. Filipino rebels to end 40-year fight
Kuala Lumpur, Malaysia–The Philippine government and the country's largest Muslim rebel group completed talks Saturday on a deal to end four decades of fighting which has killed tens of thousands of people and helped foster Islamic extremism in Southeast Asia. The good news is that two groups negotiated after 40 years of war. It's a start.
4. Nations make strides on nuclear security
This article reports that the number of nations with appreciable fuel for atomic bombs has dropped from 32 to 25. The U.S. dropped a point to tie with Britain in 11th place. Also the U.S. has not ratified two nuclear accords despite making commitments to do so. Well, that part isn't very good news! Australia remains in first place and North Korea in last. The countries now off the list of those having bomb-making fuel were Austria, Czech Republic, Hungary, Mexico, Sweden, Ukraine and Vietnam. That's the good news.
5. Pope to visit Holy Land to boost relations
Vatican City . . .the three-day visit in May also underscores Francis' close ties to the Jewish community, his outreach to Muslim's and the Vatican's longstanding call for peace between Israel and the Palestinians. . . .Francis said he would visit Amman, Bethlehem and Jerusalem in May. The good news is this religious leader is taking personal action in the name of peace between ancient enemies. That seems hopeful.
6. Mars attracts, as hopefuls are culled . . .1,058 applicants picked for the next round of nonprofit's colony plan
Los Angeles Last summer, more than 200,000 applicants applied for a one-way ticket to Mars. This week 1,058 of them were selected to move on to the next round. . . . Anyone over the age of 18 was invited to send in a video in which they explained why they wanted to go to Mars, and how they felt about never returning to Earth. They were also asked to describe their sense of humor. . . . The pool of selected applicants includes 472 women and 586 men. . . .half are under age 35 but 26 are over the age of 56. The oldest to move on to the next round is 81. . . . contenders hail from 107 countries . . .United States is most heavily represented with 297, Canada is second with 75. Launch of the final team to Mars: 2025. It's hard for me to say why this feels like good news, but it's something to do with the optimism, courage, curiosity, and goldang big-dawg foolhardiness required. SOMEBODY needs to get out there in the universe and take a look around!
7. Korea reunions aim to ease tense time
Seoul, South Korea South Korea's president proposed Monday that the two Koreas improve their tense relations by resuming the reunions of families separated since the Korean War. . . North Korean leader Kim Jong Un urged that Seoul and Pyongyang create "a favorable climate for improved relations" in a New Year's Day speech. This is obviously good news. The Korean War was a part of my long-ago youth, and that is just way too darned long to keep families from crossing borders to visit each other.
8. The truth is out there . . .Like Carl Sagan before him, Neil deGrasse Tyson wants to "transform how we think about science"
An article by Lynne Shere in Parade Magazine announces a new version of the wonderful old Carl Sagan program, Cosmos. Surely you remember it fondly! This one, honoring Carl, is called Cosmos: A SpaceTime Odyssey. The new guy says that he wants to help you "understand your relationship to other humans, to the rest of the tree of life on Earth, to the rest of the planets in the universe, and to the rest of the universe itself. I want it to get inside your skin. I want you to be so affected that the worlds looks completely different. This starts in March. Watch for it, 'cause this is really good news.
So that wraps up Good News January. I am not discouraged and will continue to scour my morning read for proof that we are headed in the right direction, in the direction of love and compassion.


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A friend of mine posted a photo on Instagram a few weeks ago of this galette here. It was incredible. You can check it out here, so you know what I'm talking about and we're on the same page. I loved it so much that I left him a comment telling him that I was going to steal it. So steal I did, and took about a week to recreate it, with some minor changes here and there and now I'm sharing it with you all because how could I not? This Heirloom Tomato Caprese and Corn Galette is like summer wrapped around a savory pie crust and you need to make it today because you won't regret it, I promise you that. A switched it up with a black pepper and thyme crust, heirloom tomatoes, and a sun dried tomato pesto because nothing goes better with tomatoes than even more tomatoes, am I right?
I always say that galettes are the food of the summer because they can be either savory or sweet and most often than not they're made with summer berries or summer produce. It's that epitome of farmer's market meals because you can visit your local farmer's market (or just local grocery store) and grab whatever looks fresh and vibrant in the produce section at the time and place it on a pie crust and call it good. It doesn't get any easier than that. If you really want to make things easier for yourself, then by all means, use a store-bought pie crust and then just slice and arrange the tomatoes and cheese and corn on top. We won't tell anyone. But really, if you can, try the black pepper and thyme crust yourself because it really is incredible. Also, a big thanks to Alex for the inspiration!


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Police super-recognizer Idris Bada quoted:
"I've seen a blurry image in infrared and I knew who it was straight away," he says. "The size of his head, his posture, the way he walks. We all have our signature moves. That's when the memory banks click in."
Storr, Will Human image banks: meet the Met's 'Super recognisers'. Telegraph. March 26th 2013.
Super-recognition researcher Josh P. Davis quoted:
"Writing in an upcoming issue of Psychologist, the scientists say super recognizers may be extra efficient at extracting information about a face, especially if viewed in action. Davis says that previous studies have shown that people can extract more information about a face if it's moving, as opposed to looking at a still image. But he says the police officer super recognizers seem to have a disproportionate advantage over others for gleaning information about faces as they scan video images or watch people in motion."
Gaidos, Susan Familiar faces. Science News. Web edition August 23rd 2013, Print edition September 7th 2013. Volume 184 Number 5 p.16. http://www.sciencenews.org/view/feature/id/352687/description/Familiar_faces


text: Leather Look Magazine Rack - 5 Tray €119.95 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 The leather look magazine rack is a refined design. The black leather effect laminate with a stitch finish calls positive attention to the area. Plus, you'll add a touch of class to any office or reception area with the look of leather. This wood magazine rack has the look and feel of leather but is able to withstand heavy use without wear. the rack has 5 magazine pockets that can hold 2-3 magazines each. It can be wall mounted or free standing. Dimensions: H:610cm W:37cm D:13cm. Wall fittings supplied. You may also be interested in.... Leather Look Magazine Rack - 10 Tray€185.95 Mesh Magazine Rack - 5 Tray€79.95 Brushed Metal Wall Rack by Peter Boy - 5 Tray€325.95 Item added to your basket You may also be interested in: CONTINUE SHOPPING


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Accurate and timely staging of prostate cancer (PCa) is essential for matching patients with the appropriate treatment. Noninvasive staging techniques using anatomical imaging alone are inadequate and extensive research efforts have focused on molecular imaging agents that exploit the in-vivo mechanisms of PCa. There is considerable potential for SPECT for molecular imaging of the prostate. One SPECT agent now in use is ProstaScint, an In-111-radiolabeled monoclonal antibody for detecting soft-tissue PCa metastases in the lymph nodes of the pelvis and abdomen. ProstaScint has FDA approval for the evaluation of newly diagnosed cases as well as treated patients who are at risk of recurrence, but interpretation of ProstaScint scans is challenging enough that few institutions offer the imaging test. Inherent weaknesses with the clinical protocol for ProstaScint imaging contribute to this problem. The standard SPECT systems with rotating camera heads and medium-energy parallel-hole (MEPAR) collimators to control septal penetration of the In-111 gamma rays deliver relatively poor photon sensitivity and spatial resolution. We hypothesize that practical alternatives to MEPAR SPECT are available to significantly improve ProstaScint imaging. Novel multipinhole (MPH) systems dedicated to cardiac imaging have recently shown impressive results surpassing the spatial resolu- tion and detection efficiency of parallel-hole collimation. The goal of this proposal is to investigate the utility of MPH SPECT for imaging PCa. Working in simulation, we shall apply the paradigm of task-based technology assessment to evaluate diagnostic performances. Among our objectives is the design and optimization of a novel MPH system dedicated to prostate imaging.
The specific aims of this proposal are: 1) implemention of procedures for simulating sets of clinically realistic PCa images;2) optimization of MPH SPECT designs for imaging PCa;and 3) comparison of MPH and MEPAR SPECT for diagnostic prostate imaging. A unique element of our approach is the utilization of numerical observers intended to mimic human observers for clinically realistic tasks of interest, offering the potential for accurate predictions of human performance over a wide array of clinically relevant tasks. Our long-term objective is to improve SPECT imaging of PCa. It is expected that such improvements would help not only with ProstaScint but also with future prostate-specific SPECT agents that may be introduced into the clinic. Overall, completion of the project aims could translate to improved staging and more effective management of PCa, reducing patient risks and enhancing response to therapy.
Accurate staging of the progression of prostate cancer in the body is vital for proper management of the disease, but current staging methods are inadequate. This grant will investigate new single photon emission tomography (SPECT) camera designs for improved staging via noninvasive imaging. Among the potential benefits of this study are better identification of patients who would benefit from a particular therapy, improved response to therapy and cost-effective diagnosis and treatment of prostate cancer.
|Sen, Anando; Kalantari, Faraz; Gifford, Howard C (2016) Task Equivalence for Model and Human-Observer Comparisons in SPECT Localization Studies. IEEE Trans Nucl Sci 63:1426-1434
|Gifford, Howard C (2013) A visual-search model observer for multislice-multiview SPECT images. Med Phys 40:092505


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Deputies Stop Driver With 28 License SuspensionsLocal News June 12, 2018
When a Cortland County Police officer stopped a truck on Route 13 in Cortlandville Monday morning, an uncovered load was just the start of the driver's problems.
Todd LeRoux, 38, of Syracuse, was driving with 28 suspensions on his license, according to deputies.
The felony Unlicensed Operator charge sent him to the Cortland County Jail, awaiting $300 cash bail.
LeRoux is due to return to Cortlandville Court June 27.


text: CBR1000BK 7 Report post Posted April 27, 2017 Tire shopping. Sorry to beat a dead horse, been researching best I can. Any suggestions for a liter bike? See quite a few mix n match with s21s in front and T30s in the rear. Anyone with a similar setup I'm all ears. Thanks! Quote Share this post Link to post Share on other sites


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On the Move: A Life
Posted by nliakos on August 29, 2015
by Oliver Sacks (Knopf 2015, ISBN 978-0-385-35255. I read the electronic version)
Starting in the 1970s, I think I have read pretty much everything Oliver Sacks ever wrote, from Awakenings to Hallucinations (exception: I didn't finish Migraine, which seemed to be written more for medical professionals than for lay readers, and I got bogged down in technical jargon; also, I may have missed the Mind's Eye–must get it!). There was no way I would not read this, ostensibly Sacks' final book.
All those years spent reading all those books, and I never knew very much about the man himself–in spite of reading the personal narratives A Leg to Stand On and Uncle Tungsten, so this book filled in a lot of gaps for me. I was totally surprised to learn that Sacks was a body-builder and a motorcyclist and that he took a lot of illegal drugs in the sixties. I didn't know that he is gay, either; in fact, I never thought about his sexuality at all. In On the Move, he is totally open about all these aspects of his life and more.
He also describes the writing of all those books that I loved, and I found those accounts fascinating, although someone who has not read them would undoubtedly find them less so.


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Kenan-Flagler's serious consideration to implement a $2,000 fee for incoming business administration majors is both discouraging and unsurprising.
It is unsurprising in that the decision perfectly follows market rationality. Administrators roughly calculate how much extra money the school can demand from students and how many students can pay this amount.
This logic ignores the fact that such a fee increase will further prohibit less wealthy students from earning a degree in one the university's top ranked undergraduate departments.
Instead of living up to the university's mission to provide a rigorous and accessible public education, Kenan-Flagler has opted to continue their impulse toward self-segregation, disconnecting their programs and facilities from large swaths of the student population and perpetuating the economic advantage of wealthier undergraduates. UNC students not enrolled in business degrees cannot even access the McColl Building after certain hours.


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Chicago-based Grant Thornton LLP (FY13 net revenue of $1.3 billion) named Doreen Griffith OMP of its Greater Bay Area practice. She will oversee the growth and operations of the San Francisco and San Jose, Calif., offices.
Most recently, Griffith served as national MP of tax services for Grant Thornton. She succeeds Jeff Pera, who will continue to support the Greater Bay Area practice. Since joining Grant Thornton in 2003, Griffith has served in a number of roles with increasing responsibility, including tax practice leader for the Greater Bay Area and regional tax partner. She also served as the OMP of the firm's Honolulu practice.
"Doreen brings to this role proven leadership coupled with an extensive knowledge of tax issues and a deep familiarity with many of the regional resources available to best serve our clients," says Jacqueline Akerblom, MP of Grant Thornton's West region. "She will be integral in maintaining and building upon the momentum that Jeff established for the firm's Greater Bay Area practice during the last 11 years."


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Break Free From Your Monkey-Mind!
Get out of your own way...
Bye Bye Monkey Mind!
Shannon's meditation really helped me get grounded and get out of my "monkey mind!" I didn't even think I could meditate, but it's so easy to follow along with Shannon's peaceful tone and the calming background music, that I had no trouble quieting my mind and finding peace and clarity!
Soothing and Restorative!
A beautiful guided meditation that is soothing and restorative. Thank you!
Creativity Just Flows!
I've been using this meditation daily for almost two weeks and I just love it! It has helped me stop over-thinking my task and tap into my creativity in a way that makes it just flow!


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Cherokee Nation Housing Authority Taking Applications for Low-rent Housing
TAHLEQUAH, OKLAHOMA — The Housing Authority of the Cherokee Nation has low-rent housing openings in the Oklahoma towns of Hulbert, Westville, Porum and Pryor.
Applications are now being accepted at all housing authority offices, will be mailed upon request or can be downloaded at http://www.hacn.org/ under the low-income rental housing tab.
Proctor Heights in Hulbert and Bushyhead Heights in Westville have one-, two- and three-bedroom apartments available. Wheeler Estates in Porum has two-bedroom apartments, and Cherokee Heights in Pryor has three-bedroom apartments available for rent.
The apartment complexes are managed by the Housing Authority of the Cherokee Nation and provide affordable homes for low-income families. Rent is not to exceed 30 percent of the family's adjusted income. Security deposits will be waived.
Eligibility requirements are as follows:
A member of the family must be a citizen of a federally recognized tribe
Household income must be below 80 percent of the national median income
Must be able to pass a background check
Preference will be given to Cherokee citizens who are elderly, disabled or handicapped and to working families.
For more information, call the Housing Authority of the Cherokee Nation at 918-456-5482.


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They say that in the restaurant business the most important things are "location, location, location." Well that's bullshit. I'd know because all three of my restaurants failed despite excellent locations. Both "Let's Taco 'Bout Our Feelings" And "God's Flan For Me" were right next to a Mexican cemetery yet didn't last more than a month. But it was the failure of "Artichoke Me, Daddy" (located in the vicinity where all those grisly strangling murders happened) that really made me look back and ask myself: where did I go wrong?
Continue reading "Why My Restaurant, "Artichoke Me, Daddy," Failed"


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LNG is coming to town!
LNG is coming to town!
For the last few years, LNG has been causing quite a stir in the Rotterdam port. In September 2011, when the first Dutch LNG import terminal 'Gate Terminal' opened, in November 2011, when the Dutch "Argonon" became the first LNG-powered barge in operation in Europe, and with the new build of the LNG Breakbulk Terminal starting within the next six months, enabling the transshipment of LNG to trucks and inland vessels.
Why all this interest in LNG? Well, in light of the EU's ambitious climate and energy targets (by 2020 the emission of CO2 must be reduced by 20% compared to the emission in 1990) and the strict emission regulations for the shipping industry coming into effect in 2015, the transport sector cannot continue to solely rely on HFO/diesel/gasoil as its main fuel source. The fact that the air pollution in the Netherlands, with its port of Rotterdam, is one of the worst of Europe attests to that.
Despite the fact that CO2 reduction has become more of an issue the last years, also in the transport sector, we have got a long way to go if we want to meet the serious targets that we have set ourselves. LNG seems to be one of the key ingredients to make our race to the bottom successful. Not only does the 'liquid' LNG take in 600 times less space than gas, making is very useful to transport by truck or inland vessel, but it is also much cleaner than HFO/diesel/gasoil (a reduction of the emission of particulate matter and NOx by 90%).
In light of the recent LNG developments, one might wonder how the upcoming LNG market is going to affect everyday life. With the build of the aforementioned Break Bulk Terminal, an opportunity is created to distribute the LNG from the import "Gate Terminal' directly into trucks and inland vessels, and thereby transporting LNG further into the mainland. As such, a new supply chain is created, literally into our backyard. Of course this is only effective if more terminals and filling stations are created in the mainland and along the rivers, just like the currently existing bunker facilities. If such LNG facilities become more common, the number if LNG-powered trucks and (inland) vessels will increase, bringing us closer to meet our climate targets. In this regard, the ambitious view of the National LNG Platform (a periodically held meeting of LNG and transport interests) is to have at least 50 inland vessels, 50 seagoing vessels and 500 trucks operating on LNG by 2015. For day-to-day life this means that increasingly more LNG-powered trucks and vessels will travel our roads and rivers, and therefore also trucks and vessels carrying LNG as a cargo, in order to meet the demands for LNG as a fuel.
When a (relatively) new product like LNG is going to be used (either as a fuel or as a cargo), the existing national and international legislation must be reviewed to that end. When it comes to LNG as a cargo, it is currently still considered a class 2 (UN1058) 'dangerous good' under i.a. the European Agreement concerning the International Carriage of Dangerous Goods by Road (ADR) and by Inland Waterways (ADN). On a national level, the by-laws of the port of Rotterdam contain separate proceedings for vessels carrying LNG. However, under the current regulations (Rhine Vessel Inspection Regulation and the Inland Ship Rules) LNG is not allowed to be used as a fuel for inland vessels in the Netherlands, because it has a flashpoint below 55 degrees Celsius. This is a clear example of legislation having to catch up with reality. However, the authorities are willing to make exceptions in order to stimulate the use of LNG, as they have done with the "Argonon". And even the UN Economic Committee for Europe has introduced a Task Force LNG in order to make the necessary amendments to the international regulations. These developments show a hopeful willingness to move forward, not only by those few business pioneers, but also on a national and international level.
All in all, LNG is an innovative product that – due to its continuing development – creates exciting business opportunities for all port related players. And although the legal and practical issues surrounding LNG have not yet been completely figured out, the first steps have been taken. As a law firm we took our next step by taking part in the National LNG Platform. Who follows?


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Friends: It was nearly dark when we finally crested a nearly 9,000 foot ridge dappled with patches of snow all around. It was our final obstacle, we hoped, after a day of climbing from Lake Van, that would at last reveal Mount Ararat, a moment we had been anticipating for hours and days. But no, there was another high ridge hiding Ararat.
We were exhausted and momentarily debated whether to camp at the summit amidst a rugged field of lava clumps. With hardly a flat, smooth spot to be seen it was much preferable to keep riding, not only in search of flatter and smoother terrain but also to descend to a warmer, more protected, elevation. Nor were we quite ready to give up on spotting Ararat before our day was done.
We bundled up for the wickedly cold descent. Less than a minute after we began our plunge, the towering white crown of Ararat popped into view through a crook in a ridge, nearly taking our breath away. Even though it was more than 30 miles in the distance, it loomed so large, lookıng as regal as any mountain on the planet, we felt as if we could reach out and embrace it.
With only its upper snow-covered reaches visible, it appeared suspended in midair. A few minutes later, three miles after we began our descent, we rounded a bend and had a full, unobstructed view of its entire majesty. Its summit wasn't a dramatic pinnacle, but rather the flattened cone of a volcano. It has been more than 150 years since it has last erupted. It had the aura of any of the greats--Everest, Denali, the Matterhorn. It was clearly not just another mountain.
Just beyond us down the road we could see a village, while to our left a flat plateau with a small quarry over its lip offered just the campsite we were looking for. It was near dark, and cold enough, with patches of snow about, that what shepherds might have been out would surely be home by now, always a concern when we're searching for a campsite here in Turkey.
Ararat gradually faded in the dimming light, but it greeted us with supreme magnificence the next morning. It was such a thrilling site, we felt as triumphant as if we had reached its summit, not an altogether false premise after our strenuous climb of the night before.
We'd had a cold night at over 8,000 feet, but surprisingly not our coldest. Our water bottles didn't even freeze, as they have previously. We could have continued on for another thirty miles or more for an even closer look at this 17,000 foot deity that is said to have been the resting place for Noah's Ark, but we had no need for that, especially since it would take us through a city that had a bad reputation and would require a hard climb back.
We warmed up quickly climbıng the three miles back to the summit, then had a forty mile descent to Lake Van. We sped past the army post five miles from the summit where we'd stopped the evening before to fill our water bottles. It was our first military road check, even though we have passed quite a few military bases. Our first impulse was how much we would like to take a picture of the tank and the barb-wired encampment at this outpost just over a ridge from the Iranian border, but knew our cameras would be confiscated if we did. But when the commanding officer pulled out his huge Nikon and asked to take our picture and took a series of shots with clusters of soldiers, we pulled out ours and asked if we could have shots of them. They were honored.
Earlier that day the owner of an Internet cafe in Çaldıran wanted a photo with the both of us as well. The adults treat us with great hospitality and respect, in contrast to the adolescents. The non-adults are like pesky rodents circling about us hoping for a handout while waiting for us to let down our guard so they can quickly pounce and grab whatever they can, such as the cycling gloves David had swiped. They torment us in whatever way they can. Lonely Planet warned that kids in certain cities could make life a misery for travelers. We'd both encountered bothersome kids before, but nothing like these.
A shepherd wearing a mask for warmth and carrying a rod to control his sheep charged us screaming "money, money," as we climbed the 9,000 foot ridge, causing us a bit of a fright. He looked all the part of a bandito. With no traffic within sight up or down the road he might have been tempted to waylay us. We would have certainly been at his mercy.
We are continually vigilant and on edge. A teen-aged boy gave both David and I a hard swat with his fist as we passed he and his cronies later in the day as we pedaled along on the flats. Our first reaction to Lake Van was how beautiful it was and how nice it was going to be to spend several days cycling around it. David imagined returnıng with an inflatable kayak and paddling its circumference. But now after all the harassment we have endured, such a proposition would be unthinkable.
Even before we entered the simmering Kurdish country and were inflicted by the stone throwing and aggressive behavior, the frequent "friendly" horn toots and vociferous "Hallos" from people along the road were rubbing our nerves raw. David wished Turkey's president could join us for a day. He was certain that if he experienced all the abrasive "whoopıng and hollering" of everyone along the road, he would issue an instant national order to his citizens to let up and be more considerate to tourists, as tourism is so important to the country's economy. "These people need to get a grip," David griped.
After we returned to Lake Van, about an hour before sunset we saw two tourıng cyclists approaching us climbing a hill we were descending. It was the Polish couple we had chatted wıth for a few minutes as we biked into Göreme while they were on their way out. They had taken a more southerly route out of Göreme than we did into the more populous and dangerous regions of Kurdish country, even passing through Diyarbakir, city of two million, that we had been warned by many to steer clear of. It had been a horror for them, continually harassed by feral youth.
They were so shell-shocked and urban-weary, they feared venturing into Van after taking the ferry across the lake, so stayed at a campground on its outskirts. They were eager to get out of Turkey and into Iran, just a couple days more of riding for them. They were struggling to do 50 miles a day, as they were extremely overloaded. "Its our first tour, we didn't know better," they explained. Nor did they know enough to send home about half of what they had brought. They were impressed by our lighter loads, especially David's ultra-light load. David is the rare touring cyclist making do without front panniers. Just as us, they had not seen another touring cyclist in the past two weeks.
We have 75 miles to complete our circuit of the lake and then another 200 miles to Syria. It will be a relief to be out of this Kurdish sector of Turkey despite its exceptional rugged beauty.
Later, George
Saturday, November 13, 2010
Edremit, Turkey
Posted by george christensen at 1:41 AM
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A Superior Court judge made several errors in law when he ruled that provincial legislation to shrink Toronto council violated Charter rights, appeal documents filed by the Attorney General's office say.
On Monday, Justice Edward Belobaba released his decision on a constitutional challenge to Bill 5 reversing the Doug Ford government's plan to decrease council to 25 wards from 47, because he found the law violated Charter rights to free expression and arrived mid-campaign.
Belobaba had predicted, prior to hearing evidence from several complainants, including city council, that the unsuccessful side would appeal any decision he reached.
The PC government filed a notice of appeal Wednesday, the same day it introduced legislation that invokes the notwithstanding clause of the Canadian Charter of Rights and Freedoms to override the court decision.
Attorney General Caroline Mulroney said the government would appeal Belobaba's decision, but the looming Oct. 22 election date left it no option but to use the Charter tool to ensure council was reduced to 25 wards in time.
"There will be irreparable harm if the 25-ward election is not allowed to proceed as scheduled on Oct. 22," the appeal documents, which have not been tested in court, say. " In this case, the city clerk had already advised council that (as of Aug. 20, 2018) she was prepared to run a 25-ward election on Oct. 22 and that reverting to a 47-ward election would cause her concerns regarding the integrity of the election and the possibility that the results would be controverted. In the circumstances, it is in the public interest for the 25-ward election to be held on Oct. 22."
Belobaba found that the Charter right to freedom of expression, including political expression, was violated by Bill 5 and ordered that the 47-ward plan be reinstated for the 2018 election.
The notwithstanding clause allows the Ontario government to pass and implement a new version of Bill 5, now called the Efficient Local Government Act (Bill 31), regardless of the judge's findings.
Still, the provincial government is planning on making a case in court that the judge impugned on it's authority under the Constitution, to give and take away Toronto's power to set its own ward boundaries.
"(Belobaba) gave weight to irrelevant factors and erred in holding that Ontario had not put forward sufficient evidence of a pressing and substantial objective, despite the clearly stated evidence of the legislature's objectives reflected in the legislative debates and in the record overall," the notice of appeal says. "The application judge overstepped what was necessary to remedy the constitutional breach he found, as well as the proper role of the court, in mandating that the pre-existing 47-ward regime be revived."
Toronto council, which voted 27-15 in favour of challenging the province's 25-ward edict, was in a special meeting Thursday to discuss its use of the notwithstanding clause.
Ford has said that reducing the size of council will save money and ensure that major initiatives like transit projects move expeditiously through council meetings.


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2019 Jeep Cherokee
If you are looking for a stylish SUV that features the latest and greatest technology options, the 2019 Jeep Cherokee may be right for you!
The new 2019 Jeep Cherokee features improved cargo space and a new striking exterior and rear, so you can enjoy the Jeep life. Take a look at the benefits of the new and improved 2019 Jeep Cherokee, then visit Krause Family Ford to start living the "Jeep Life!"
Explore New Jeep Cherokee's for Sale near Kennesaw
The Jeep Cherokee has received a refreshing facelift for 2019. Not only does it look great on the exterior, it offers drivers and passengers a comfortable ride with exceptional off-roading capabilities.
Most notably need for 2019 is the forward-looking design which features a new front fascia, hood, LED headlights and new fog lamps. On the back, the 2019 Jeep Cherokee features a new lightweight hands-free power liftgate and updated taillights. We also love the option of five new wheel designs, which includes upgraded 19-inch wheels.
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Inside, the Jeep Cherokee consists of the same comfort features as its previous generation. You'll have the option to opt for the larger 8.4-inch Uconnect touchscreen with Navigation, which will allow you to hook up your phone and send text messages, make hands free calls and more.
The new 2019 Jeep Cherokee boasts plenty of cargo room, exactly 25.8 cubic feet of space to be exact. Fold down the 60/40 split rear seats and get access to 55 cubic feet. The available power liftgate allows you to open the trunk with your foot for easy access.
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For 2019, Jeep is offering a new engine for the Cherokee — a 2.0-liter turbocharged inline four-cylinder that delivers an impressive 270 horsepower and 295 pound-feet of torque.
The other engine options will remain the same, including the base 180 horsepower 2.4-liter and the 3.2-liter V6, which offers 271 horsepower and 239 pound-feet of torque.
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Explore our inventory of new 2019 Jeep Cherokee's online, then schedule your test drive by calling us at (877) 397-0257 or use our online contact form.


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|Little Lord Occy
Production Code:
Broadcast Number:
Written by:
Jim Pfanner
Directed by:
Berny Wolf
Broadcast Information
USA Premiere: October 8, 1988
Syndication Premiere: October 28, 1988
Paired With
Battle Of the Gadgets
"Little Lord Occy" is the 10th episode of Season 4. Occy is discovered as the long lost pet of the Snorkhamptoms, so Occy goes to the mansion where he will be trained to act sophisticated and proper.
Plot Summary
The snorks are playing basketball outside, and Occy helps Allstar win the game. A limo pulls up to them, and out ccomes Hobbs, the butler to Lord Snorkington, searching for an octopuppy. He then examines Occy until he finds a star on the back of his head. He then claims Occy's the long lost octopup of the Snorkhamptons, as the star is supposed to be his birthmark. He then claims him to be "Little Lord Occy." He offers them a local playground to be built on the property if they choose to give Occy away to Lord Snorkington. None of the snorks are too happy about this.
However, Junior and the others convince Allstar to give Occy away and have him live the life of luxury. Casey says Occy would go to an obedient school, and Junior thinks the playground would help boost the town. Allstar's not into the whole idea, so they make him pretend to dislike Occy. But Occy overhears Allstar doing this, and so he sadly leaves home. Hobbs stops the limo and offers him a ride. Occy is then introduced to Lord Snorkington, and they agree that his training as an obedient octopup begin immediately. Occy is then trained in how to eat correctly, he rides seahorses, learns how to dance, balances books on his head, and has to learn opera. But they sense Occy misses Allstar and the others, so they choose to invite them to the Snorkutante Ball.
Meanwhile, the snorks are watching the local playground be built, but all Allstar can think about is Occy. Daffney receives the big invitation to the Snorkutante Ball, which is exciting for them. They go there, and Occy has to act proper and sophistiated for them. He refuses to dance with any of the snorks, but Lord Snorkington can sense Occy longs to return to them. He then says nothing can replace a friednship between a snork and his octopup, so he lets Occy return to Allstar. Everyone happily dances, and Hobbs too with Occy's fleas.
Background Info
- First time snorkberries were mentioned (like Smurf's smurfberries?!)
- We learn that Occy is the long lost pet of the Snorkhamptons
- Continuity Error 1: The Snorkutante Ball isn't like the Debutante Ball in Me JoJo, You Daffney
- Continuity Error 2: It was revealed that Occy once belonged to the Snorkhamptons, although Junior helped him hatched out of an egg in Junior's Octopuppy
Memorable Quotes
Allstar: That's my Occy.
Junior: *Mocks* That's my Occy, that's my Occy! *they growl at each other*
Daffney: Allstar Seaworthy, how can you be so shellfish?
Snorks: Get her!
Daffney: *blushes* Well... you know what I mean.
Junior: I still think they should've named it after me - it was my idea!
Casey: Poor Occy. He doesn't get to have any fun.
Allstar: Boy, is he snooty? He's ignored us the whole time we've been here.
Junior: What's the problem? He's rich now, he's supposed to be that way.
- Allstar's room changes shape throughout
- Hobbs' eyelids are uncolored at one point
- How did Allstar know Occy was already at the Snorkhamptons if he didn't know for sure where Occy was and whether he was okay
- Title and storyline is based off of the first children's novel Little Lord Fauntleroy, and Hobbs was named after Cedric's friend in the book
- Visit page: Little Lord Occy/Transcript
Previous: Battle Of the Gadgets


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The Hollywood movies and stars seems to sport theaterical colored contacts to achieve the same mind-blowing wickidness or sci-fiction robotic looks. Follow the trend, follow your heart's voice and wear these crazy & bold red circle lenses to spiceup the Gothic look. Find more about these circle lenses in the review shared by Vanilla Syndrome. Check out her Gothic Look with Geo Color Nine Red circle lenses.


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Interview with Chinese Londoner Lucy Mitchell, SeeWoo oriental food specialists
London celebrates Chinese New Year in a big way, with an annual Chinatown parade and a cultural festival in Trafalgar Square. For an insight into London's Chinese side, we've interviewed the Managing Director of SeeWoo, which includes a longstanding oriental supermarket in the West End.
Chinatown is packed with restaurants, but where does all that food come from? Established in 1975, SeeWoo supermarket is a big cog in the Chinatown machine. We interviewed SeeWoo's MD to find out more.
'My father Stanley came over from Hong Kong in the early 1960s as a teenager' explains Lucy. 'It was hard to get authentic Chinese ingredients, so he opened a supermarket in Chinatown. The business grew and eventually they were supplying restaurants around the country.'
SeeWoo's operation is a great insight into Chinatown life: 'We send a lorry to Chinatown at 8am, but if a restaurant has a busy lunch we can barrow-boy rice sacks to them at 3pm.'
With an English mother from London and a Chinese father, Lucy personifies the city's cultural melting pot – so what does she like to eat? 'I love dim sum and all Chinese, Vietnamese and Korean food. There's a lady called Thuy in Angel who runs The Little Viet Kitchen and I love her food.'
Lucy is also the founder of the UK's Golden Chopsticks Awards. 'The Asian food scene is massively changing, especially in London' says Lucy. 'Filipino food is spreading and in the next few years that will become bigger. I'm excited for that!'
SeeWoo, 18-20 Lisle St, WC2H 7BE
020 7439 8325 | www.seewoouk.com
Read our list on how to celebrate Chinese New Year 2019 in London and discover more Chinese Londoners in our interview series below:
Ellen Chew, Rasa Sayang restaurant founder
Reverend Paul Lau and Eugene Ling, St Martin-in-the-Fields church
Ruby Wu, Dim Sum Master for Ping Pong
An-Ting Chang, CEO for Chinese Arts Now


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India Delhi Office
RZ-C-93, Block-C, Dabri Extn.
East, New Delhi -110045.
Phone:(0) 99100 77151
Phone:(0) 90153 20764
Email: email@example.com
Follow on:
Facebook, Twitter
I Need to Register
Nagaland People's Front, popularly called the Naga People's Front or NPF is a regional political party. Its mass base is primarily in the states of Nagaland and Manipur. The NPF operates along the political ideology of Regionalism, abiding by the principles of democracy and fraternity as practised in the state of Nagaland.
The Naga People's Front (NPF) is a regional political party in Nagaland and Manipur, India. It headed the Nagaland government with the Bharatiya Janata Party, as part of the Democratic Alliance of Nagaland since 2003. Dr. Shurhozelie Liezietsu is the president of the party.
The Nagas of South Nagalim have been struggling very hard to achieveNaga Integration under the banner of United Naga Council (UNC). The UNC has launched a series of movements in pursuance of its political demands: (1) Non-cooperation movement against the Manipur Government (2) Boycott of the Manipur District Council election (3) Severance of ties with the Manipur Government and (4) Demand for an Alternative Arrangement outside the purview of Manipur administration. In addition to these, the All Naga Students' Association, Manipur (ANSAM) had also initiated a drastic move seeking affiliation of the schools in the Naga areas of Manipur to Nagaland Board.
The Naga people boycotted the first and second general elections in India in 1952 and 1957 for the political problem in Nagaland. But when an Interim Government was installed with the Naga people as counselors, it set the stage ready under the system for the General Election to come. Under the circumstances, organizing a new political party was the only answer to set up the democratic system of allowing everyone to contest in the election and not to go by the resolutions of the Tribal Councils. It is also to be noted that the 16-point memorandum failed to stop bloodshed in Nagaland. Therefore, the political party had to declare a policy to work for a final political settlement of the Indo-Naga political problem.
From 2003 to 2008, the Nagaland People's Front ruled the state in alliance with the BJP in the Democratic Alliance of Nagaland (DAN), where it is the main constituent of the coalition. The state was ruled under the able leadership of the NPF's most significant leader Neiphiu Rio. In 2004, the NPF absorbed the Nagaland Democratic Party, one of the significant political outfits in the state. This made the NPF all the more influential in the state. Though the NPF supports the BJP-led NDA in the politics of the state, it has no coalition with either the NDA or the Congress-led UPA at the Centre.
The Election Symbol of the Nagaland People's Front, as approved by the Election Commission of India, is "Cock". This election symbol is drawn on a tri-coloured flag of the party, which has a blue top strip, followed by a white middle strip and a red bottom strip, all of which are of equal sizes. The cock is a small Indian bird, which is usually famous for being an early-riser, welcoming the morning sun. The party, representing its ideology through the cock symbol, welcomes light and happiness for the people of Nagaland. Its strong motto, by which the party wholly abides, is "fido non armis", which means "by faith, not by non-violence". This indicates the passionate commitment of the party towards the cause of the Nagas, at the expense of not killing or physically abusing the innocent lives of the Nagas.
Contact Details and Address
Head-Office Address of NPF - P.R. Hills Junction, Post Box No. 565, Kohima-797001, Nagaland
All Indians
Scope National
Scope State
Minister House C-1 Someshwar Tenament, Ranip,
Ahmedabad, Gujarat - 382 480
Minister House New Delhi, India
Minister House 3rd, Level, A-Wing, Delhi Secretariat, I.P. Estate, New Delhi
Minister House C-4, M.S. Flats, B.K.S. Marg, New Delhi - 110 001
Minister House C-1/36, Pandara Park,New Delhi - 110 011


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I'm one of the millions of people around the world who can not log into, post or share on their Instagram and Facebook pages right now. According to the usage tracker on my phone, one might say I've made my way on the "naughty" overuser list and this mandatory break is a gift.
Without meaning to, I slip my phone up and into my hand and begin scrolling out the people around me; allowing myself to move moments and people down the "gets the most attention" list. I don't ever think this is what I'm going to do, but it seems my time gets snatched up with obligations and things I must do and then when I have a moment to respond to that text, respond to that person's post or even check to see how many people responded to my blog post that I just posted four seconds beforehand, it bleeds over into quality time with my person.
I'm not giving up on myself in this area yet. I've already begun to improve but would like to get even better at detaching from the digital and connecting to the person/people before me.
Which is why I so appreciate intentional times on the calendar to date my spouse.
Mister's phone isn't as "smart" as mine which also means he isn't as attached to his device (nor is his bill as high). It benefits us. It helps us meet in the middle and forge new ground. And this is also is why I make sure I no longer post about our dates while they're happening.
I snap a shot and turn my attention quickly back to the moment I'm blessed to be experiencing and make time to write and share about it here, much later. Which is what I'm doing right now about our most recent "date your spouse" date!
Date #06: Watch a Movie Marathon and eat a huge ice cream sundae
It was my turn to plan. And if you've been around here for the "Date Your Spouse" segments, then you know the person who picks the date, plans the date.
One of the things I try to do when planning the date is to really think about my husband's preferences and gear the date more toward him. Since he's not in control, I want to choose things that I think he'll enjoy and appreciate; things that will let him know I was thinking of and making attempts to prefer him.
My husband's sweet tooth makes it almost impossible to disappoint him in the area of desserts. On our weekly run to Target, we threw caution to the wind and chose the kind of gelato we liked (Sea Salt Caramel + Coffee). I got the ingredients to make homemade whipped cream from Joanna Gaines' Cookbook, "The Magnolia Table" and made the brownie from scratch while halfway through our first of two movies.
Mister really likes Matthew McConaughey and his movies.
I wanted to be sure he got to watch one of those, so I went through our Amazon Prime account before we sat down and let him choose one of the few that popped up. So, Mud, it was. And that's all I'm going to say about that.
Well, I will say one more thing.
We did not finish the movie or the sundae that night. We saved both and enjoyed them over the weekend.
We had a date! And it was memorable and we learned about one another. I learned that my husband considered that sundae one of the best desserts he's ever had (he's 54yo y'all!) and I think he learned and is now certain of my preference for dates at home.
I'm already looking forward to our next date this coming weekend (#60) where we'll be staying home again and talking about our future. Good things come when couples set aside time together to dream and cast vision in their marriage.
What about you?
You may not be in the same season as us or choose the same things on a date like this, but what from here can you take and make work for you and your person – even if right now, that person is just you?


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Rising China? Responsible China?
The Chinese Communist Party's Third Plenum released its plan for reforms, including moving toward the free market in allocating resources, abolishing prison reeducation, easing the one-child restriction for some families and eliminating local control over the judiciary. Despite such guidance on reforms, though, the plan is also designed to strengthen the party's control, writes journalist Frank Ching.


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A chlamydial type III translocated protein is tyrosine-phosphorylated at the site of entry and associated with recruitment of actin
Edited by Stanley Falkow, Stanford University, Stanford, CA (received for review April 21, 2004)
The obligate intracellular bacterium Chlamydia trachomatis rapidly induces its own entry into host cells. Initial attachment is mediated by electrostatic interactions to heparan sulfate moieties on the host cell, followed by irreversible binding to an unknown secondary receptor. This secondary binding leads to the recruitment of actin to the site of attachment, formation of an actin-rich, pedestallike structure, and finally internalization of the bacteria. How chlamydiae induce this process is unknown. We have identified a high-molecular-mass tyrosine-phosphorylated protein that is rapidly phosphorylated on attachment to the host cell. Immunoelectron microscopy studies revealed that this tyrosine-phosphorylated protein is localized to the cytoplasmic face of the plasma membrane at the site of attachment of surface-associated chlamydiae. The phosphoprotein was isolated by immunoprecipitation with the antiphosphotyrosine antibody 4G10 and identified as the chlamydial protein CT456, a hypothetical protein with unknown function. The chlamydial protein (Tarp) appears to be translocated into the host cell by type III secretion because it is exported in a Yersinia heterologous expression assay. Phosphotyrosine signaling across the plasma membrane preceded the recruitment of actin to the site of chlamydial attachment and may represent the initial signal transduced from pathogen to the host cell. These results suggest that C. trachomatis internalization is mediated by a chlamydial type III-secreted effector protein.
Chlamydia trachomatis is a Gram-negative obligate intracellular bacterium that is a leading cause of sexually transmitted diseases and blindness worldwide (1). Chlamydiae have a biphasic developmental cycle characterized by an infectious but metabolically inactive extracellular form, called the elementary body (EB), that initiates infection by attaching to and inducing uptake by the host cell. Once internalized, chlamydiae remain within a membrane-bound vacuole termed an inclusion, where the organism differentiates into the larger, metabolically active reticulate body. Reticulate bodies replicate and differentiate back to EBs before release at the end of the developmental cycle.
EBs attach to and enter cultured eukaryotic cells so efficiently that the process has been termed parasite-specified phagocytosis (2). Despite the importance of this event to chlamydial pathogenesis, little consensus exists regarding the identity of the chlamydial ligands and respective host receptors (for a review, see ref. 3). Considerable evidence suggests that electrostatic interactions mediate attachment with heparan sulfate-like proteoglycans involved in an initial, reversible interaction with the eukaryotic host cell for many, but not all, strains and species of chlamydiae (4–9). Recent studies using chemically mutagenized cell lines distinguished a subsequent, irreversible secondary binding step in the entry process, although the receptor was not identified (8, 10). Entry of C. trachomatis requires participation of the actin cytoskeleton and involves the formation of actin-rich pedestals at the site of entry (11). It is likely that chlamydiae initiate signaling cascades that recruit actin to promote internalization.
Tyrosine phosphorylation of host and/or bacterial proteins have been implicated in signaling pathways triggering the entry of many intracellular pathogens (12). Studies of tyrosine phosphorylation in response to chlamydial infection have described several proteins; a triplet of 64, 66, and 68 kDa, and proteins migrating at 97 and 140 kDa (13). Similar studies reported tyrosine phosphorylated proteins of 75–85 kDa and 100 kDa (14). In both cases, tyrosine-phosphorylated proteins were observed in association with EBs by immunofluorescence. Although definitive identification of the proteins was not achieved, they were presumed to be of host origin.
We have confirmed tyrosine phosphorylation in response to C. trachomatis infection but find only a single unique protein that is phosphorylated in a multiplicity-dependent fashion. This protein was partially purified and identified by matrix-assisted laser desorption ionization-time of flight MS as a chlamydial protein of unknown function that appears to function in signaling of the cytoskeletal rearrangements that lead to the endocytosis of EBs.
Materials and Methods
Organisms and Cell Culture. C. trachomatis serovars L2 (LGV 434) or D (UW3-Cx) were grown in HeLa 229 cells as described and EBs were purified by Renografin (Squibb) density gradient centrifugation (15). Fluorescent CMTMR-labeled EBs were prepared as described (11, 16). HeLa cells were infected at a multiplicity of infection (MOI) of 100 (unless otherwise noted) for both serovar L2 and D in Hanks' balanced salt solution (HBSS; Invitrogen) at 4°C. The inoculum was removed (time = 0), cells were rinsed with HBSS, and warm media were added for the additional times indicated.
Cloning and Sequencing. Full-length CT456 from L2 genomic DNA was PCR-amplified and cloned in pCR-Blunt (Invitrogen). Sequencing was performed by the DNA-sequencing facility at Iowa State University (Ames) by using M13 universal and reverse primers and progressive step primers. Sequences were compiled and analyzed with macvector and assemblylign. The L2 CT456 DNA sequence was deposited in GenBank (accession no. AY623902).
Antibodies. The C-terminal 580 aa of CT456 was cloned into pRSET-B (Invitrogen) and expressed as a His-tagged fusion. The fusion protein was purified on a nickel column (Invitrogen) and used as an immunogen for the production of specific polyclonal antibodies in female New Zealand White rabbits as described (17). Other antibodies used were anti-phosphotyrosine mAb clone 4G10 and 4G10 coupled to agarose (Upstate USA, Charlottesville, VA), anti-Rickettsia rickettsii ompB mAb 13–3 (18), anti-C. trachomatis major outer-membrane protein (MOMP) mAbs L2-I-10 and L2-I-45 (19), anti-Flag M2-alkaline phosphatase (Sigma-Aldrich), Texas red-X phalloidin (Molecular Probes), and anti-C. trachomatis LcrH (K.A.F. and T.H., unpublished data). Secondary antibodies for microscopy were AlexaFluor 594-conjugated anti-rabbit IgG (Molecular Probes), FITC-conjugated anti-rabbit IgG (Zymed), and tetramethylrhodamine B isothiocyanate-conjugated anti-mouse IgG (Zymed).
Analysis of Type III Secretion. Type III secretion of CT456 by heterologous expression in Yersinia pseudotuberculosis was performed by using type III competent YPIII pIB102 WT or type III null YPIII pIB68 (yscS) strains expressing CT456-Flag-tag (FT) fusions as described (20, 21).
Transfections. Full-length CT229 was PCR-amplified from L2 genomic DNA and ligated into pcDNA3.1/CT-GFP-TOPO (Invitrogen). HeLa cells were seeded on 12-mm glass coverslips in 24-well plates to obtain a monolayer of ≈50% confluency. Transfection of CT456-enhanced GFP (EGFP)-C3 (BD Biosciences, Palo Alto, CA) or CT229-GFP constructs were performed by using Lipofectamine 2000 according to the manufacturer's instructions (Invitrogen). Expression from the transfection vectors proceeded for 24 h at 37°C before fixation.
SDS/PAGE and Immunoblotting. Proteins were separated on SDS-7.5% polyacrylamide gels (22) and transferred to 0.45-μm Transblot nitrocellulose membrane (Bio-Rad). Immunoblots were developed by using Super Signal West Pico chemiluminescence reagent (Pierce). For immunoprecipitations, cultures were lysed in cold immunoprecipitation buffer [50 mM Tris, pH 8/150 mM NaCl/1% Nonidet P-40/1% sodium deoxycholate/0.1% SDS/1 mM Na3VO4/1 mM NaF/protease inhibitor mixture (Complete mini, EDTA-free, Roche Diagnostics)]. Supernatants were incubated overnight at 4°C with antibody and precipitated with protein A-Sepharose (Sigma-Aldrich). For 2D gel electrophoresis, cultures were scraped into buffer and acetoneprecipitated; the pellet was solubilized in rehydration buffer {7 M urea/2 M thiourea/2% (wt/vol) 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate/5% (vol/vol) 2-mercaptoethanol/1 mM NaF/0.2 mM Na3VO4} and resolved in the first dimension by using the IPGphor isoelectric focusing system with pH 3–10 drystrips according to the manufacturer's instructions (Amersham Pharmacia Biosciences). The second dimension was separated and transferred to nitrocellulose as above.
MS. CT456 was enriched by immunoprecipitation from L2-infected HeLa cell lysates by using 4G10, separated by SDS/PAGE, and visualized by the SilverQuest silver stain kit (Invitrogen). Gel slices were processed as described in detail in the Supporting Materials and Methods, which is published as supporting information on the PNAS web site. Matrix-assisted laser desorption ionization-time of flight spectra were collected by using a Voyager-DE STR mass spectrometer (PerSeptive Biosystems, Framington, MA).
Microscopy. Infected cells on 12-mm glass coverslips were processed as described (23), except that all buffers contained 1 mM Na3VO4/1 mM NaF. Confocal images were acquired on a Perkin–Elmer UltraView spinning disk connected to a Nikon Eclipse TE2000-S microscope with a ×60, 1.4 numerical aperture oil-immersion objective. All images were processed with imagej (written by Wayne Rasband at the National Institutes of Health and available by anonymous FTP from http://zippy.nimh.nih.gov) and/or photoshop software (Adobe Systems, Mountain View, CA). Images are representative of at least three separate experiments.
Live-Cell Confocal Microscopy. Cos-7 cells were grown on 25-mm glass coverslips and electroporated with 500 μg of actin-EGFP-C1 (24) per well with the BTX 35-mm Petri Pulser (Genetronics, San Diego) connected to an ECM 630 generator (Genetronics) set at 150 V/975 μF in serum-free Optimem (Invitrogen). Fresh serum-containing medium was added, and cells were allowed to express overnight. 4G10 was labeled by using the Alexa Fluor 660 protein-labeling kit (Molecular Probes) according to the manufacturer's instructions and was microinjected into ≈50 cells per coverslip as described (25), except that Oregon-green dextran was not used. GFP-expressing and microinjected cells were identified by spinning-disk confocal microscopy, and, after an initial image was taken, cells were infected with CMTMR-labeled L2 EBs. Images were acquired every 2 s for 10 min per coverslip. Results are representative of more than four experiments.
Immunoelectron Microscopy. Confluent HeLa cells on Thermanox coverslips were infected with L2 (MOI ≈ 300). Coverslips were washed two times with HBSS and then processed as described (17), except that cells were stained with 4G10 overnight at 4°C, and PBS washes, permeabilization, and primary antibody solutions included 1 mM Na3VO4 and 1 mM NaF. Sections were observed at 60 kV on a Philips CM-10 transmission electron microscope (FEI Co., Hillsboro, OR). Images were acquired with an AMT digital camera system (Advanced Microscopy Techniques, Chazy, NY) and processed with photoshop software.
A Single Unique Tyrosine-Phosphorylated Protein Is Induced During Infection with C. trachomatis. Immunoblotting with the mAb 4G10 was used to identify tyrosine-phosphorylated proteins in response to C. trachomatis L2 infection. In contrast to previous studies that had identified two or more tyrosine-phosphorylated putative host proteins in C. trachomatis-infected cultures (13, 14), we observed only a single unique tyrosine-phosphorylated protein that increased in prominence in a multiplicity-dependent fashion during infection with C. trachomatis (Fig. 1 A and C ). This phosphoprotein was not detected in lysates of purified EBs (data not shown) and was observed during infection with either C. trachomatis serovars L2 or D, although the apparent M r of the D protein was slightly higher. Reactivity with 4G10 was abolished by treatment of the lysates with calf intestinal alkaline phosphatase (data not shown) and occurred even in the presence of the tyrosine kinase inhibitor, genistein, at 200 μM final concentration (Fig. 7, which is published as supporting information on the PNAS web site). EBs allowed to adhere at 4°C then shifted at 37°C to trigger entry resulted in rapid tyrosine phosphorylation of the protein (Fig. 1B ). Signal was detected by 5-min after temperature shift and was maximal at 30–60 min after infection. Tyrosine phosphorylation was detectable for at least 24 h although the intensity diminished at later time points. Chlamydial transcription and translation were not required for this phosphorylation as incubation in the presence of rifampicin or chloramphenicol did not inhibit its occurrence (Fig. 7). Two-dimensional gel electrophoresis demonstrated that the phosphoprotein is highly acidic (Fig. 1D ).
We confirmed tyrosine phosphorylation in association with internalized EBs by immunofluorescence microscopy with mAb 4G10. As shown (13, 14), immunoreactivity was observed soon after initiation of infection by warming L2-infected cultures to 37°C and appeared to stain the EBs in a polar fashion (Fig. 8, which is published as supporting information on the PNAS web site). By immunoelectron microscopy with a horseradish peroxidase-conjugated secondary antibody, reaction product could readily be visualized on the cytoplasmic face of the plasma membrane, even as EBs remained extracellular at the apex of the characteristic actin-rich pedestals formed during internalization (11). Immunoreactivity remained localized to one hemisphere of the nascent inclusion after endocytosis (Fig. 2).
Identification of CT456. Immunoprecipitation with 4G10 was used to enrich for the tyrosine-phosphorylated protein from C. trachomatis L2-infected cultures. A single comigrating band corresponding to 4G10 reactivity was visualized on silver-stained SDS-polyacrylamide gels. No comigrating bands were observed from uninfected cultures immunoprecipitated with 4G10 or infected cultures precipitated with an irrelevant, isotype-matched mAb. The unique band was excised and identified by matrix-assisted laser desorption ionization-time of flight MS as the hypothetical protein CT456 from the C. trachomatis serovar D genome sequence (26). The corresponding gene from C. trachomatis L2 was cloned, sequenced, and found to exhibit 94% identity to the serovar D protein sequence (by bestfit alignment, http://molbio.info.nih.gov/molbio/gcglite/compare.html). The predicted molecular mass of the protein product is 103 kDa with a pI of 3.92. CT456 thus appears to migrate aberrantly on SDS/PAGE. Fig. 3A shows the serovar L2 CT456 protein sequence with peptides identified by MS demarcated. An interesting structural feature of CT456 is the presence of six near-identical tandem repeats of ≈50 aa in length (Fig. 9, which is published as supporting information on the PNAS web site). The purified C-terminal domain (downstream of the repeats) of serovar L2 CT456 was used as an immunogen to inoculate rabbits for antibody production. The anti-CT456 antiserum reacts with the protein immunoprecipitated by 4G10, thereby confirming the identity of the tyrosine-phosphorylated protein in C. trachomatis-infected cell lysates as CT456 (Fig. 3B ). Conversely, immunoprecipitation of L2-infected cultures with anti-CT456 similarly precipitated CT456, which was reactive with 4G10, whereas immunoprecipitation of equivalent amounts of Tarp from L2-infected cultures maintained at 4°C confirmed that Tarp is present but not phosphorylated in EBs (Fig. 9). The anti-456 antiserum also reacted with a prominent band of the corresponding size from either L2 or D EBs (Fig. 3C and data not shown); thus, the nonphosphorylated precursor is preexisting in EBs.
CT456 Is Secreted by a Type III System. Many Gram-negative pathogens deliver effector proteins to the host cell by a type III secretion system (27). Because tyrosine phosphorylation is observed on the cytoplasmic face of the host plasma membrane at the site of intimate attachment of EBs and on the nascent inclusion membrane of internalized EBs, it is likely that CT456 is translocated across the plasma membrane. To test this, we used a heterologous type III system used previously for identifying suspected chlamydial type III effectors (20, 21). Type III competent (WT) or null (yscS) Y. pseudotuberculosis alone or expressing full-length CT456-Flag-tagged (FT) fusions were grown in media inductive (-Ca2+) or repressive (+Ca2+) for type III secretion, and cultures were fractionated into cell-free culture supernatants and yersiniae-containing whole-cell pellets. Immunoblot analysis (Fig. 4) of parent and yscS cultures revealed essentially equal CT456-FT-specific signals in +Ca2+ (lane 5) and -Ca2+ (lane 7) whole-cell fractions but not in vector-only controls (lanes 1–4). CT456-FT was detected in the -Ca2+ supernatant (lane 8) of WT but not yscS Yersinia cultures. This outcome was not due to bacterial lysis, because the Yersinia cytoplasmic protein LcrH was detected only in whole-cell pellet samples and not released into supernatant fractions. Additionally, expression of CT456-FT had no adverse effects on the ability of Yersinia to properly regulate and secrete YopD, an endogenous type III substrate. Therefore, these data indicate that CT456-FT is specifically recognized and secreted as a type III substrate.
CT456 Translocation and Actin Recruitment. To characterize exposure of CT456 on the EB surface, HeLa cells were infected at 4°C, then shifted to 37°C for 30 min to trigger entry. Cultures were stained with anti-CT456 and counterstained with anti-MOMP mAb L2-I-10 to localize EBs. Surface-associated EBs show little or no reactivity with anti-CT456 at 4°C, supporting the concept that the protein is not exposed on the EB surface (Fig. 5A ). After temperature shift, CT456 staining colocalized well with anti-MOMP staining. The staining pattern at 37°C with anti-CT456 also corresponded to 4G10 staining (Fig. 5A ). Thus, CT456 does not appear to be exposed on the EB surface but is translocated such that it becomes antibody-accessible during the entry process.
Because CT456 phosphorylation occurs rapidly and is observed at the site of pedestal formation, we investigated whether it might be involved in signaling for actin recruitment. A three-color live-cell experiment was performed on Cos7 cells expressing actin C-terminally fused to enhanced GFP (actin-EGFP) and microinjected with fluorescently labeled 4G10 antibody. Fluorescent CMTMR-labeled L2 EBs were added and spinning-disk confocal microscopy was used to acquire images at 2-s intervals. With time 0 defined as the first sighting of an EB in the image, 4G10 staining was first observed by 30 s, adjacent to the EB, with robust recruitment of actin observed by 90 s (Fig. 5B ). CT456 phosphorylation detected by the intracellular pool of 4G10 confirms that CT456 is translocated and exposed to the cytoplasm. Further, the sequence of events suggests that CT456 phosphorylation may play an important role in recruitment of actin and initiation of EB entry.
Transfected CT456 Is Tyrosine Phosphorylated and Recruits Actin. To further examine the role of CT456 in recruitment of actin, full-length CT456 from serovar L2 was expressed as a C-terminal EGFP-tagged fusion protein in HeLa cells. CT456-EGFP expressed in HeLa cells formed aggregates that stained with phalloidin to indicate recruitment or bundling of actin (Fig. 6). CT456-EGFP also colocalized with 4G10 staining to demon-strate phosphorylation of the transfected protein. HeLa cells expressing EGFP alone (data not shown) or a chlamydial inclusion membrane protein (CT229) fused to GFP showed no colocalization with phalloidin nor reactivity with mAb 4G10 (Fig. 6). Thus, it is likely that CT456 phosphorylation is important in the initiation of as-yet-unidentified signaling pathways that initiate the entry process by triggering recruitment of actin.
Although chlamydiae enter nonphagocytic cells very efficiently, the molecular mechanisms involved have remained elusive. An initial electrostatic interaction involving heparan sulfate-like glycosaminoglycans is believed to precede an irreversible engagement with unknown receptors that leads to internalization (5, 8, 10). The earliest known signaling of chlamydiae to the host cell is tyrosine phosphorylation of unknown proteins in immediate apposition to the internalized EBs (13, 14). We find that this signal is transduced to the cytoplasmic face of the plasma membrane when the chlamydiae are still extracellular and have identified a prominent tyrosine-phosphorylated protein as the C. trachomatis hypothetical protein CT456. CT456 is present in EBs but not exposed on the EB surface and appears to be translocated into the host cell by the chlamydial type III secretion system. Once exposed to the cytoplasm, CT456 is rapidly tyrosine-phosphorylated by unknown mechanisms. Tyrosine phosphorylation of CT456 is temporally and spatially associated with recruitment of actin to the site of chlamydial entry. Furthermore, CT456-EGFP expressed in HeLa cells is tyrosine-phosphorylated and recruits or bundles actin. CT456 thus appears to initiate or participate in signaling events that regulate the actin recruitment, which ultimately leads to internalization. We have therefore named CT456 "Tarp", for translocated actin-recruiting phosphoprotein.
A striking feature of L2 Tarp is the presence of six tandem repeats of ≈50 aa. C. trachomatis serovar D is highly homologous to that of L2 but contains only three repeat units (Fig. 9). The repeat region is absent in other chlamydial strains or species. C. trachomatis strain MoPn (28), Chlamydia psittaci strain GPIC (29), and Chlamydia pneumoniae (28) all possess orthologs of CT456, but the greatest similarity occurs within the C-terminal domain downstream of the repeat region. The phosphorylated residues have not been defined but the repeat region of L2 is rich in tyrosine, containing 26 of the 31 residues present in the protein. The large number of potential tyrosine phosphorylation sites may explain the strong reactivity of Tarp with the 4G10 antibody even though the protein appears to be of low abundance in EBs. Despite the absence of repeats, C. pneumoniae entry involves the formation of identical short pedestal structures indistinguishable from those observed with C. trachomatis serovars L2 and D (K. Wolf, E.R.F., and T.H., unpublished observations). The function, if any, of the distinct domains of Tarp may provide unexpected insights into the different pathologies of chlamydial species and serovariants.
Phosphorylated Tarp appears to remain cytoplasmically exposed on the inclusion membrane at one side of internalized EBs for several hours after entry (data not shown). However, Tarp does not have any predicted transmembrane domains, thus it is unclear how the association with EBs through the inclusion membrane is maintained. An unusual protein structure may yield a domain that could span the membrane; however, Tarp does not become associated with membranes in the Triton X-100-soluble fraction after secretion and remains associated either with the cytoskeleton in cell debris or with the EBs themselves. Neither high salt (500 mM NaCl) nor reducing agents increased the Triton solubility of cell-associated Tarp (D.R.C. and T.H., unpublished observations). Identification of the means of Tarp attachment to EBs may shed light on Tarp function(s).
Although chlamydial EBs have been considered metabolically dormant, these findings suggest that they are primed for the translocation of preexisting effectors, such as CT456, by type III secretion. CT456 is transcribed from mid to late cycle in the chlamydial developmental cycle (30), and unphosphorylated Tarp is detected in purified, infectious EBs. In addition, inhibitors of chlamydial transcription or translation have no effect on Tarp phosphorylation. Tarp does not appear to be exposed on the surface of EBs because it is resistant to trypsinolysis on purified EBs (Fig. 10, which is published as supporting information on the PNAS web site) and is not reactive with an anti-Tarp antibody but becomes exposed after association of EBs with host cells at 37°C. Specific type III secretion from a heterologous bacterial host, Y. pseudotuberculosis, further suggests that Tarp is a chlamydial type III-secreted effector molecule. Whereas an active process on the part of extracellular EBs challenges some paradigms of this developmental stage, it should be noted that EBs contain an exceptionally high concentration of ATP (≈40 mM) (31) that is presumably retained for activities required before the developing reticulate bodies become fully metabolically active.
Several examples exist of bacteria that secrete type III effectors as a means of altering cytoskeletal processes. For example, enteropathogenic Escherichia coli (EPEC) translocates its own receptor, Tir, into the host cell where it inserts into the plasma membrane and binds to the EPEC outer-membrane protein intimin, which is required for intimate attachment to the host cell surface (32). Tyrosine phosphorylation of Tir recruits Nck and leads to the formation of a stable actin pedestal (33–35). In contrast, chlamydiae are internalized rapidly after recruitment of actin, whereas EPECs typically remain extracellular at the apex of the pedestals. EPEC also requires Nck for actin recruitment and pedestal formation, whereas chlamydial internalization functions independently of Nck (R.A.C., S.S.G., and T.H., unpublished observations). Salmonella and Shigella inject several type III effectors that interact with multiple host signaling pathways, including activating Rho/Rac/Cdc42 GTPases (36–40). This process leads to distinctive transient membrane ruffling that results in entry of the organism. Chlamydial entry depends on Rac activation (41), thus it is tempting to speculate that Tarp may trigger Rac signaling pathways that culminate in actin recruitment. However, the sequence similarity between chlamydial Tarp and other known bacterial secreted effectors is minimal, suggesting that any functional similarities arose independently.
The initial stage in the interaction of chlamydiae with host cells is a critical time in the infectious process. Tarp translocation and its tyrosine phosphorylation appear to be one of the first signals to the host cell to actively subvert host processes for parasite purposes. We believe Tarp plays a role in the recruitment of actin to initiate entry, but other functions are possible. Although we have no evidence that Tarp is involved in attachment, per se, the fact that it is rapidly translocated but remains associated with internalized EBs suggests it may be important in forming the irreversible attachment thought to follow initial electrostatic interactions (8). Additional, unidentified signaling pathways may also be activated that together initiate entry of the organism or promote events that favor intracellular survival after entry. For example, the nascent inclusion appears to be nonfusogenic and shows no demonstrable interaction with the host cell until de novo synthesized chlamydial proteins initiate interactions with the cytoskeleton and exocytic lipid-trafficking pathways (23, 25). Because Tarp is exposed to the cytoplasm and remains associated with EBs after entry, a role in controlling interactions of the early inclusion is possible.
We thank Janet Sager for excellent technical assistance; Drs. Robert Heinzen, Olivia Steele-Mortimer, and James Carroll for helpful discussions; Dr. Greg Plano for sharing the anti-YopD antibody; and the members of the Hackstadt laboratory for critical reading of the manuscript.
↵ * To whom correspondence should be addressed at: Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT 59840. E-mail: thackstadt{at}niaid.nih.gov.
This paper was submitted directly (Track II) to the PNAS office.
Abbreviations: EB, elementary body; MOI, multiplicity of infection; Tarp, translocated actin-recruiting phosphoprotein; MOMP, major outer-membrane protein; EGFP, enhanced GFP; FT, Flag-tag.
Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. AY623902).
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text:
This story first appeared on CNBC:
The fatal attack on a pro-European British lawmaker just a week ahead of the key Brexit referendum vote has shaken politicians and voters across the U.K.
While this is the first time in nearly a quarter of a century that a U.K. politician has been murdered, the case bears startling similarities to the assassination 13 years ago in Sweden of pro-EU Anna Lindh.
Days before Sweden would vote on whether to join the euro in 2003, a 46-year-old lead campaigner for Sweden's to adopt the euro was stabbed while shopping in a Stockholm department store. She had been shopping for clothes for a euro zone debate scheduled that evening. The next day, on September 11, Social Democratic foreign minister Lindh died from her injuries.
Both sides of the Swedish political campaign suspended their activities following the news, with TV ads campaigns cancelled and billboard and print media ads withdrawn.
On the following Sunday, Swedes rejected proposals to adopt the common currency.


text:
by Ian Marshall, ITTF Publications Editor
Paralympic Games gold medallists, World champions and Asian champions are plentiful; leading the way for the men is Feng Pangfeng, in Class 3 the holder of all three titles, for the women it is Mao Jingdian, in Class 8, she is also the owner of the full set.
Likewise for the women, in Class 3 Li Qian and Xue Juan are both present, the respective gold and silver medallists in Rio de Janeiro; later, alongside Liu Jing, they were the Women's Team Class 1-3 gold medal winners. Additionally, Li Qian is the reigning World Champion, Xue Juan the winner early this year at the Asian Para Championships in Beijing.
Major contenders for honours; it is the same for their female colleagues Zhang Miao and Zhou Ying. Competing in Class 4, Zhang Miao is the current World and Asian champion, having been the silver medallist in Rio de Janeiro; alongside Zhang Bian and Gu Gai, Zhou Ying struck gold in Women's Team Class 4-5 in the Brazilian city.
Notable names and in the women's events there are more; Liu Meng who won gold in Class 9 in Rio de Janeiro competes, as does Lei Lina the reigning World champion, the player Liu Meng beat in Paralympic Games final. Similarly in Class 9, Xiong Guiyan, the Asian champion is on duty as in Class 10 is Yang Qian, the Rio 2016 silver medallist.
Current champions abundant in the women's events in Suphan Buri; it is very similar in the male competitions.
Additional to Fang Pangfeng, further Rio 2016 Paralympic Games Chinese national team medallists appear on the Thailand entry list. Gold medallists, Cao Ningning in Class 5 and Zhao Shuai in Class 9 are on duty, as is Zhai Xiang who partnered Fang Pangfeng to Men's Team Class 3 gold. Also, Guo Xingyuan, silver medallist in Class 4 and Yan Shuo, bronze medal winner in Class 7, competes.
Furthermore, recently, competing in the same Class, Yan Shuo was crowned Asian champion in Beijing an honour also bestowed on the shoulders of Zhan Dashun in Class 5 and Zhao Yi Qing in Class 9.
A strong entry from China to say the least with Paralympic Games gold medallists plentiful; from other shores there are also names who have gained that distinction.
Notably the names of Slovakia's Jan Riapos, Class 2; Germany's Jochen Wollmert, Class 7 and most importantly from a host nation perspective, Thailand's Rungroj Thainiyom, Class 6, all gold medallists in London in 2012 appear on the entry list.
2017 Thailand Para Open: Latest Results and Forthcoming Matches