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It all started in 1740. Inès Pradel de Lavaux is the 10th generation of one of the most influential Bordeaux wine families. Nowadays, the family owns 22 prestigious Chateaux in the finest appellations of Saint-Emilion, Pomerol, Fronsac and Bordeaux. Embracing her roots, she travels around the world to find true wine treasures. These wine treasures then became the "Inès Secret Wine Collection". All her wines are from wine regions with heritage, where the tradition of wine-making and growing grapes is passed from generation to generation.
There are more than just One great wine region in the world!
Imagine - One brand, Six countries, different types of wines for you to explore. More than just wine, Imagine wine label each tells a story of its country of origin – from the Gallus rooster to Australian kangaroo – Some wines are blended, others are derived from a single variety, but all wines are packed with great care and are produced with the biggest passion. Don't know where to start with your wine journey? Follow Imagine.
Because you deserve it! Inès Sweet Collection wants to bring out your inner goddess. Find her and embrace her. Know that you are special! The sweet collection has the ideal sweet wines for different occasions. It is not only for when you want to enjoy your time alone, but also when you want to share your happiness with your friends. J'adore is the joie de vivre, and it celebrates your independence.
Let Capolavoro take you down the memory lane - Capolavoro is the Italian word for "Masterpiece", it is also the wine that brings your wine journey to the fullest. A selection of superb wines from Italy's finest wine regions. All handcrafted to perfection and delicately bottled with Italy's most well-known sceneries that visualizes the taste of each wine.
Amsterdam, the city that hosts artistic heritage, elaborated canal system, and carries on with its 17th century Golden Age legacies. Brewed with the traditional recipe, Amsterdam beers is dedicated to this ever innovative, always open-minded city. Drink to the city that never sleeps. Proost!
WineBridge B.V.
Veemkade 528, 1019 HE Amsterdam,
The Netherlands
Phone: +31 20 2232 177
WineBridge Limited
3806 Central Plaza 18 Harbour Road,
Hong Kong
Phone: +85 281 977 225
Fuzhou WineBridge International Trading Co., Ltd.
Fuzhou Mawei Luoxing East Road No.2
Haixia Jingmao Plaza No.1, 4TH FLOOR A-7
Fujian, Peoples's Republic of China
Phone: +86 186 6794 2102


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Black Crow is Tel-Aviv based label and parties' line operating under the same name, bringing to Tel-Aviv a tasteful selection of the world's top class techno DJ's and lives acts.
Black Crow, the label, is set to counter-match this high standard with its musical output.
Behind the label stand production/DJ duo Deep'a & Biri together with Shay Peled metal artist from Tel Aviv. The concept of the labels is to combine fresh art & sound.
Black Crow's rooster of artists includes Deep'a & Biri, The Analog Roland Orchestra, 2030, and Hakimonu amongst others.
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subset: commoncrawl


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Platform Question
The platform question helps the client to see things as they currently exist. By focusing on what has already been achieved, people can often get a different, more positive perspective both on their current situation ("It is not all bad!") and on their possible future. Once they take a more optimistic view of their situation, they become more hopeful that they will be able to achieve their change goals.
Examples of Platform Questions are:
Examples of Platform Questions are:
- What have you already achieved?
- What is already there?
- What has helped to bring you to your current position?


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Northern Waters with Tom Kerwin
We have one more new painting by Tom Kerwin today! Very fitting to all the rain and water happening outside, but in a much more pleasant way. Come visit us to see this piece in person, and catch a preview of some of the new work we're receiving for our upcoming show!


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Hacking Productivity: How to Get More Done in the Time You Have
Lunch and Learn
28 February 2019
12:30 pm to 1:30 pm
Academy Xi, 48 Druitt Street, Sydney 2000
Event Details
With a finite quantity and the inability to create more of it, time is our most valuable resource. In an era where we're faced with plenty of distractions and noise, we're constantly pushing ourselves to do more.
How do you get stuff done — and done effectively, in the limited amount of time we have?
At our lunch and learn, Ben Fitzpatrick will take you through:
- How he got 12 hours of work in less than 8 hours as a new dad
- How to prioritise the most important tasks
- What you can do to minimise distractions
- How your team can embed practices to increase your efficiency of output
Ben Fitzpatrick
Head of Operations at Webprofits
Ben Fitzpatrick is Head of Operations at Web Profits, a fast-growing Australian Digital Growth Consultancy. Ben specialises in developing processes and systems across the organisation to drive continuous growth – with a focus on efficiency, productivity and quality of service.
Dates and fees
28 February 2019
12:30 pm ~ 1:30 pm
Academy Xi,
48 Druitt Street,
Sydney 2000


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merging documents in openoffice after the break…
merging documents in openoffice…
yeah, i know… "really hot topic." not as wonderful as, say, "megan fox caught nude! w00t!" or anything like that.
seriously, i was dabbling with openoffice (btw, openoffice is a free – yes, free office productivity suite for your computer – think "windows office" except for free & you're close enough) when i stumbled upon a disturbing reality: i had no way of merging two documents together.
let's go over symantics for a moment – "merging two documents together," in my mind, means "a + b = ab." that's my definition of merging. apparently, i found out that merging also means "version 1 of a document + version 2 of that same document = version 1.5 of the document." i know, it's as lame as i wrote it but that's how they use the word "merge."
so, after going on line & not finding the solution, performing unsuccessful trial by error crapola that didn't work (that's why it was unsuccessful, btw), i stumbled upon the solution. here is that solution. are you ready?
- go into either of the documents that you want merged with another document.
- place the cursor where you want the other document to begin (in front of the other document, behind, in between, wherever…).
- go to the "insert" menu, then drop down to "file" (last option in "insert" menu).
- go to the file you want inserted.
voila. you are done. two documents "merged."
yeah, not as exciting as an 8 x 10 glossy of one of megan fox's nipples, but pretty nice nonetheless. consider yourself educated.
Tags: openoffice


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- Mac
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We came home 3 weeks ago, our 4th visit to Skala, we drive all over this lovely island and we usually spend at least 2 days in Assos. And we walked up to the castle as usual - having written last year to Holiday Which about the dangerous, thoughtless and selfish advice to drive up! Invalidated car insurance too! We are 73. Snorkelling is great, venture into the cave opposite the village and then follow the rocks along...Assos was the most beautiful village we have ever seen, but what have they done to it? Several ugly apartments right on the skyline not to mention an ugly, large notice advertising the North Beach Cafe! My wife and feel that we have been so lucky to know Assos before this ugly new building. Please don't spoil the village completely.... a huge disappointment to us, we were both so sad. I feel that we may never go back.
We've stayed in Skala 4 times, Tara, Skala, Captains and Paspalis hotels. All very good in different ways but Paspalis probably best for kids and Poolside Meals. Bit noisy at times. Greek beaches usually shelve too quickly for small children. I snorkel (ex Diver) so prefer Sami to Agio Ephymia road for rocky coves. Don't miss the Old Skala walk. And Assos, bvefore they spoil it! At Sami have a look at Antisamos, Corelli Italian Camp site but a super bay. Ancient Sami which we found years ago but now being uncovered...Acropolis they call it. (Think of Ulysses). Try St.Thomas beach if you can park. St George's Castle too. Older folk should contemplate the Italian Memorial beyond Argostoli to 9,250 Italian dead - just like Corelli but worse in fact, a true and tragic story.


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You are currently browsing the tag archive for the 'Lake Geneva WI' tag.
I am again privileged to be part of the Haddon Robinson Study Retreat (Map of Participants — Don't miss Ireland!). This year we are studying the book of Isaiah and our guest lecturer is Tim Mackie of The Bible Project. Watch the Bible Project on Isaiah 1-39 and Isaiah 40-66!
Each year a group of pastors gather for a week of intense study Covenant Harbor Camp near Lake Geneva, WI . Our format is simple. We invite a world-class scholar to teach us on one or more books of the Bible. As we are taught on a technical level, we collaborate to envision how to preach the Scripture we are studying to our congregations.
We were inspired by Dr. Haddon Robinson to begin this retreat. Haddon is one of the most influential teachers of homiletics (preaching) in the English speaking world in the last 100 years. Most of us who are part of this retreat studied under Haddon. All share a commitment to the clear proclamation of God's Word.
For the rest of Chris Braun's blog…


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Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells
Tutak, W and Giri, Jyotsnendu and Bajcsy, P and Simon, C G (2016) Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells. Journal of Biomedical Materials Research Part B: Applied Biomaterials. ISSN 1552-4973 (In Press)Full text not available from this repository. (Request a copy)
Recent work demonstrates that osteoprogenitor cell culture on nanofiber scaffolds can promote differentiation. This response may be driven by changes in cell morphology caused by the three-dimensional (3D) structure of nanofibers. We hypothesized that nanofiber effects on cell behavior may be mediated by changes in organelle structure and function. To test this hypothesis, human bone marrow stromal cells (hBMSCs) were cultured on poly(ε-caprolactone) (PCL) nanofibers scaffolds and on PCL flat spuncoat films. After 1 day-culture, hBMSCs were stained for actin, nucleus, mitochondria, and peroxisomes, and then imaged using 3D confocal microscopy. Imaging revealed that the hBMSC cell body (actin) and peroxisomal volume were reduced during culture on nanofibers. In addition, the nucleus and peroxisomes occupied a larger fraction of cell volume during culture on nanofibers than on films, suggesting enhancement of the nuclear and peroxisomal functional capacity. Organelles adopted morphologies with greater 3D-character on nanofibers, where the Z-Depth (a measure of cell thickness) was increased. Comparisons of organelle positions indicated that the nucleus, mitochondria, and peroxisomes were closer to the cell center (actin) for nanofibers, suggesting that nanofiber culture induced active organelle positioning. The smaller cell volume and more centralized organelle positioning would reduce the energy cost of inter-organelle vesicular transport during culture on nanofibers. Finally, hBMSC bioassay measurements (DNA, peroxidase, bioreductive potential, lactate, and adenosine triphosphate (ATP)) indicated that peroxidase activity may be enhanced during nanofiber culture. These results demonstrate that culture of hBMSCs on nanofibers caused changes in organelle structure and positioning, which may affect organelle functional capacity and transport.
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|bone marrow stromal cell;cell morphology;nanofiber;polymer scaffold;stem cell;stem cell niche
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Biomedical Engineering
|Department of Biomedical Engineering
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Donald Trump proposes USD 54 billion increase in defense spending.
Washington: President Donald Trump's first budget proposal includes a substantial $54 billion dollar increase in defense spending, with corresponding cuts in foreign assistance and other non-military spending.
Highlighting what Trump has described as a "public safety and national security budget," the plan raises national defense spending — already the highest in the world — by almost 10 percent.


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Metastasis, the process by which cells spread from the primary tumor to a distant site to form secondary tumors, is still not fully understood. Although histological techniques have provided important information, they give only a static image and thus compromise interpretation of this dynamic process. New advances in intravital microscopy (IVM), such as two-photon microscopy, imaging chambers, and multicolor and fluorescent resonance energy transfer imaging, have recently been used to visualize the behavior of single metastasizing cells at subcellular resolution over several days, yielding new and unexpected insights into this process. For example, IVM studies showed that tumor cells can switch between multiple invasion strategies in response to various densities of extracellular matrix. Moreover, other IVM studies showed that tumor cell migration and blood entry take place not only at the invasive front, but also within the tumor mass at tumor-associated vessels that lack an intact basement membrane. In this Commentary, we will give an overview of the recent advances in high-resolution IVM techniques and discuss some of the latest insights in the metastasis field obtained with IVM.
Complete surgical resection of the primary tumor is still one of the most efficient therapies for cancer. Unfortunately, cancer can progress to a stage at which tumor cells leave the primary tumor and spread to a distant organ to form a secondary tumor, a process referred to as metastasis. Complications caused by metastases are the major cause of cancer-related death, but this process is not fully understood.
Initially, it was thought that spread of tumor cells and subsequent metastasis formation are late events in tumor progression; however, more recently it was realized that it might instead be an early event (Hüsemann et al., 2008; Klein, 2009). In either case, tumor cells have to acquire certain traits that allow them to escape from the primary tumor site and home in on and colonize a secondary site (Fig. 1). These gained properties, such as survival, invasiveness and motility, allow tumor cells to move into the surrounding tissue, where they enter blood or lymph vessels (Talmadge and Fidler, 2010; Wyckoff et al., 2000; Wyckoff et al., 2007). Once in circulation, tumor cells are transported to a secondary site, where they can grow out to form metastatic foci or become dormant (Chambers et al., 2002; Chambers et al., 1995; Nguyen et al., 2009; Talmadge and Fidler, 2010). For these colonization events to take place, tumor cells need to be able to respond to chemoattractants and growth factors, and survive in the new environment. The requirement for these traits might vary during tumor progression or among different tumor types, and the acquisition of these traits does not follow a particular order (Chiang and Massague, 2008; Nguyen et al., 2009). It is worth noting that only a small fraction of the cells present in the primary tumor have the necessary characteristics to escape from the primary site and colonize a secondary site, which renders metastasis an inefficient process (Chambers et al., 2002). To investigate these dynamic processes underlying metastasis, most studies rely on techniques that are only able to provide a static view, such as histochemistry, visual inspection of tumor size and end-stage measurements (e.g. the number of metastatic foci). In addition, these techniques analyze large numbers of cells, which obscures the signaling properties and activities of individual cells. This results in loss of crucial information concerning the adaptive properties of the few tumor cells that spread and form metastases.
Recent advances in optical methods have made it possible to visualize the metastatic process at a subcellular resolution in real time in vivo. By the 19th century, microscopes were being used to image tissue in living animals (e.g. Wagner, 1839), a technique referred to as intravital microscopy (IVM). In these early days, most IVM studies could only examine the vasculature and the microcirculation, because the optics available at that time and lack of contrast limited the visualization of other tissues. In the 1950s, the visualization of metastasis was pioneered in a rabbit ear chamber (Wood, 1958). Major breakthroughs in this field occurred in the 1990s, when intravital imaging techniques improved considerably and genetic tumor models of rodents that expressed fluorescent proteins (FPs) became available. Since then, IVM has evolved into an important tool for investigating the processes underlying cancer and metastasis (see IVM images in Fig. 1) (Chishima et al., 1997; Farina et al., 1998; MacDonald et al., 1992; Naumov et al., 1999).
Recently, a number of new IVM techniques have become available with different properties in relation to imaging depth, resolution, timescale and applications (Table 1) [for an extensive review on in vivo optical microscopy methods, see Ntziachristos (Ntziachristos, 2010)]. Techniques that are based on fluorescence microscopy provide (sub)cellular resolution and have successfully been used to image single cells in living organisms in their natural environment, such as in fruit flies, zebrafish, nematodes and small rodents (Fouquet et al., 2009; Ghosh and Hope, 2010; Le Dévédec et al., 2010; Stoletov et al., 2010). In this Commentary, we will focus on high-resolution IVM techniques used in small rodents (rats and mice), as they are the most widely used model organisms to study cancer. We will describe the advances in high-resolution IVM techniques that have made it possible to directly visualize the various steps of metastasis at cellular resolution (see Fig. 1). We will also discuss the new insights into the process of tumor cell invasion that have been obtained with such IVM studies.
Advances in IVM
High-resolution IVM techniques
There are several high-resolution IVM techniques, including optical frequency domain imaging (OFDI), spinning disk confocal microscopy and multiphoton microscopy (Table 1). In OFDI, an optical beam is focused into the tissue and reflected light is detected after interference with a reference beam. The amplitude and phase of the reflected signal as a function of time are then used to derive the optical scattering properties and thereby the tissue structure at different depths. OFDI has been used to study angiogenesis, lymphangiogenesis and tissue viability (Vakoc et al., 2009). The advantage of this technique is that it enables the imaging of substantial volumes of tissue over prolonged periods without the need for contrast agents.
At present, a number of mouse tumor models are available in which various FPs are expressed. For the imaging of tumors that express FPs, spinning disk confocal microscopy and multiphoton microscopy have been the IVM techniques of choice. In spinning disk confocal microscopy, visible light is used to excite FPs. Here, emitted out-of-focus light (outside the optical section) is eliminated by multiple pinholes, resulting in increased Z-resolution and contrast enhancement, which makes subcellular structures more easily visible. A spinning disk confocal microscope can acquire high-resolution images at high speed and low phototoxicity, therefore allowing the long-term intravital imaging of tumors (up to 30 hours) (Egeblad et al., 2008; Lohela and Werb, 2010). The downside of spinning disk microscopy is that the visible light used for excitation is scattered in the tissue, which limits its imaging depth to 100 μm. By contrast, the short pulses of infrared (IR) light used in multiphoton microscopy, with a typical wavelength range of 800–1000 nm, penetrate the tissue more than tenfold deeper compared with spinning disk microscopy (see Box 1) (Theer et al., 2003). An additional advantage of IR excitation is its ability to create a second harmonic generation (SHG) signal from non-centrosymmetric structures with highly ordered repeats, such as collagen type I fibers, an extracellular matrix component (Campagnola et al., 2002). In contrast to fluorescence microscopy, SHG does not involve the excitation of fluorophores and hence the signal does not suffer from photobleaching (for reviews, see Helmchen and Denk, 2005; Mohler et al., 2003; Zipfel et al., 2003b). SHG microscopy has been used to study the role of fibrillar collagen in tissues and organs, including skin, gut and breast (e.g. Wolf et al., 2009).
The combination of high-resolution acquisition of fluorescent tissues and deep tissue penetration has made multiphoton IVM the technique of choice for most research groups that exploit intravital imaging for tumor cell biology (for more details, see Box 1).
Advanced detection methods for simultaneous imaging of multiple cell types and molecules
Using multicolor IVM, different cell types and molecules can be visualized simultaneously, which has made it possible to study the interaction between different cell types and molecules in real time (Fig. 2A) (Bajénoff et al., 2006; Boissonnas et al., 2007; Castellino et al., 2006; Egeblad et al., 2008; Egen et al., 2008; Farina et al., 1998; Goswami et al., 2005; Lohela and Werb, 2010; Massberg et al., 2007; Wyckoff et al., 2007). Since the introduction of the green fluorescent protein (GFP) (Chalfie et al., 1994; Heim et al., 2005; Shimomura et al., 1962) and its color variants, cyan fluorescent protein (CFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP) (Giepmans et al., 2006), together with the more recently discovered FPs [e.g. photoswitchable proteins (Piatkevich and Verkhusha, 2009)], several cell types can now be genetically labeled and visualized in tumors using, for example, CFP and YFP (e.g. Sahai et al., 2005). In addition, tumor cells labeled with an FP can also be imaged at the same time as cells that are labeled with fluorescent dyes [e.g. dextran-labeled macrophages (Fig. 2)]. A specific way to label immune cells (often lymphocytes) is to isolate them and load them with fluorescent tracker dyes before transferring them back into the recipient animal. The disadvantage to this approach is, however, that the fluorescently labeled cells will constitute only a small fraction of the population compared with their endogenous, non-labeled counterparts. A simple but functional approach to accomplish contrast between different cell types and tissues is intravenous or subcutaneous injection of cell-type-specific fluorescent antibodies or other targeted tracers [e.g. for lymphatics,
Box 1. Multiphoton microscopy.
Multiphoton excitation is based on the (almost) simultaneous absorption of two low-energy IR photons, whose combined energies induce fluorescence in the visible range (see Figure, panel A). Simultaneous absorption of two photons by a fluorophore is an extremely rare event and multiphoton excitation therefore requires high excitation powers (i.e. a dense photon flux). IR lasers achieve this by condensing all photons over a time span of 12.5 ns into a 100 fs pulse (thus concentrating the photon density 1.25 × 105 times). Although the overall power is low (i.e. low number of photons per second), the photon density per pulse is sufficiently high to cause efficient fluorophore excitation in the focal plane (see Figure, panel B). Beyond the focal plane, however, the photon density is too low for simultaneous absorption by a fluorophore to occur (see Figure, panel B) (Dunn and Young, 2006; Helmchen and Denk, 2005; Zipfel et al., 2003b). Therefore, in contrast to confocal microscopy, excitation is strictly confined to the focal plane, thus avoiding the need for a pinhole to delete out-of-focus light (see Figure, panel B). This greatly enhances the detection efficiency, as the in-focus emitted light can be detected close to the objective, without the need to travel back through the scanner optical path (with loss of tissue-scattered emission at the pinhole and further losses at each filter) (Centonze and White, 1998). Furthermore, deep tissue penetration, reduced excitation of surrounding tissue, increased detection and low-energy IR light give the additional advantage of low phototoxicity and low photobleaching (Eggeling et al., 2005).
Box 1 Fig. Two-photon versus single-photon excitation.
In addition to discriminating spectral overlap between fluorophores, FLIM is also used to distinguish between the autofluorescence of various tissue types (see Box 2). Autofluorescence is the intrinsic property of cells to emit fluorescence upon excitation and is caused by its endogenous constituents, such as tryptophan, pyridinic coenzymes (i.e. NADPH), flavin coenzymes, melanin and elastin, which all have fluorescent properties [e.g. Monici and El-Gewely, 2005; Zipfel et al., 2003a]. Because most of the autofluorescent tissue components exhibit comparable excitation and emission spectra, the resulting fluorescence levels are similar and cannot be used to distinguish tissues. However, as the lifetime of autofluorescence differs between different cell types, autofluorescence lifetime measurements have successfully been used to distinguish healthy tissues from tumor tissues (Galletly et al., 2008; Provenzano et al., 2009).
Intravital imaging of proteins, proteases, signaling pathways and cellular processes in vivo
Imaging of molecular processes in living mice has been achieved by multiphoton IVM of FP fusion proteins and injectable dyes. High-resolution IVM of cells that express a fusion of FP with a receptor [e.g. ErbB2 (Dennis et al., 2007)], an adhesion molecule [e.g. E-cadherin (Serrel et al., 2009)], or an actin- or myosin-binding protein (Philippar et al., 2008; Wyckoff et al., 2006) has been used to study intracellular signaling in single tumor cells. In addition to FP fusion proteins, many biosensors that are based on measuring a change in fluorescent resonance energy transfer (FRET) (see Box 2) can be used to study intracellular signaling in vivo. The number of available biosensors is rapidly increasing, and specific FRET biosensors are already available to measure the levels of second messengers, protein–protein interactions or the activation status of proteins (e.g. Rho) [for a review on biosensors, see Morris (Morris, 2010) and VanEngelenburg and Palmer (VanEngelenburg and Palmer, 2008)]. However, as the field of FRET biosensors is still an emerging area, only a few research groups have thus far used this powerful technique in vivo. For example, FRET biosensors have been used to measure the proteolytic activity of calpain in muscles (Stockholm et al., 2005), changes in calcium concentration and myosin light-chain kinase activity in arteries (Zhang et al., 2010), and the induction of apoptosis in the skin (Bins et al., 2007), colorectal carcinomas and lymphomas (Breart et al., 2008; Keese et al., 2010).
In particular, the apoptosis sensor has been of interest for tumor biology, because tumor cells have to overcome many apoptotic signals during several steps of metastasis. The FRET apoptosis sensor consists of a CFP that is separated from a YFP by a caspase-3 recognition sequence (Fig. 2B). Caspase-3 activity cleaves CFP from YFP, leading to a loss of FRET efficiency. Indeed, when we induced apoptosis in single keratinocytes in the skin of living mice, a caspase-3-mediated loss of FRET efficiency is observed, which shows that caspase-3 activity is a good gauge of apoptosis induction in vivo (Fig. 2C,D) (Bins et al., 2007). Keese and colleagues used FRET IVM of this biosensor to study the induction of apoptosis resistance upon chemotherapy (Keese et al., 2007; Keese et al., 2010). Moreover, Breart and colleagues used this biosensor to study apoptosis of tumor cells by cytotoxic T lymphocytes (CTLs) and showed that a CTL kills, on average, one tumor cell every 6 hours (Breart et al., 2008).
Many synthetic fluorescent probes have been developed to study the intracellular and extracellular of activity of proteases such as matrix metalloproteinases (MMPs) and cathepsins in tumors. The injectable probes that have been used to measure protease activity in tumors include autoquenched protease substrates that fluoresce upon cleavage (Weissleder et al., 1999), proteolytic beacons that lose FRET upon cleavage (e.g. McIntyre et al., 2010; Scherer et al., 2008) and fluorescently labeled activatable cell-penetrating peptides (ACCPs) that translocate into cells upon cleavage (Olson et al., 2009; Olson et al., 2010). The disadvantage of these probes is that chemical knowledge is required to synthesize them, although recent commercialization of some of these probes has overcome this problem. It is also important to note that phagocytic cells, such as macrophages, might take up some of the probes despite not
Box 2. Fluorescence lifetime imaging microscopy to distinguish spectrally overlapping fluorophores.
FLIM produces images that are based on the fluorescence lifetime of a fluorophore, rather than on its intensity and color (Gerritsen et al., 2009; Wouters et al., 2001). A major advantage of the FLIM technology is that fluorophores that have overlapping emission spectra (see Figure, panel A) can be distinguished, a feature that is not possible with an intensity image. When fluorophores are excited with a femtosecond IR pulse, the fluorescence will decay exponentially with a rate that is determined by the fluorescence lifetime, which is close to zero for SHG (see Figure, panel B) and around 2 ns for Dendra2 (see Figure, panel C). In every pixel of a FLIM image, the fluorescence decay curve is measured and used to calculate the fluorescence lifetime. Many pixels of the image contain both fluorophores, and therefore will have an average lifetime that depends on the fraction of each fluorophore that is present in that pixel (see Figure, panel D). A FLIM image can be visualized by color coding the fluorescence lifetime (see color bar next to the FLIM image). In the FLIM image, fluorophores with distinct fluorescence lifetimes, such as SHG and Dendra2, will be color coded differently and can therefore be distinguished.
Box 2 Fig. Fluorescence lifetime imaging.
Long-term intravital imaging over several days
IVM has contributed to the visualization of the behavior of tumor cells in living mice. However, most IVM studies only monitor short-term movement of cancer cells because they use tumor dissection, which effectively limits experimental observations to a few hours after surgery [typically around 4 hours and after optimization up to 24 hours (Egeblad et al., 2008)]. Given the small number of tumor cells that display high motility and subsequently migrate and invade over periods that last up to days, long-term IVM techniques are needed to provide valuable information regarding these processes. By the 1990s, Lehr and co-workers had developed the dorsal skinfold chamber, which is surgically implanted on the back of a mouse, allowing multiple tumor-imaging sessions over several days (Alexander et al., 2008; Brown et al., 2001; Lehr et al., 1993). Since then, orthotopic chambers (i.e. in their natural environment) have been developed, including the so-called 'mammary imaging window' (MIW) for mammary tumors that we developed (Fig. 3A,B) (Gligorijevic et al., 2009; Kedrin et al., 2008; Shan et al., 2003) and the 'cranial imaging window' for brain tumors (Yuan et al., 1994). These imaging windows have made it possible to visualize a number of metastatic processes over several days, including the formation of new blood vessels (Abdul-Karim et al., 2003; Farhadi et al., 2005; Fukumura et al., 2010), tumor cell invasion and intravasation (Fig. 3C) (Kedrin et al., 2008), and tumor cell colonization at a distant site (Kienast et al., 2010).
Tracking of individual tumor cells over several imaging sessions
To enable long-term tracking of individual cells, reference points such as vascular and extracellular matrix (ECM) structures in healthy tissues have been used to enable retracing of the previously imaged area over several imaging sessions (Bins et al., 2007; Hayashi et al., 2007; Kimura et al., 2010; Perentes et al., 2009; Yamauchi et al., 2006; Yang et al., 2002). However, when tumorigenic tissues are observed in which the topology changes dynamically, these initial reference points might change and cannot be used for retracing. To be able to track the dynamics of areas of interest, tumor cells have been photomarked using photoconvertable fluorophores, such as Kaede (Ando et al., 2002) and Dendra2 (Gurskaya et al., 2006). These fluorophores have absorption and emission spectra that are significantly red shifted by violet light. For example, we have used this property of Dendra2 to mark regions of interest in a tumor over several days with single-cell precision (Fig. 3C) (Gligorijevic et al., 2009; Kedrin et al., 2008) and were thus able to track a large population of cells over multiple imaging sessions.
Taken together, we are now able to detect fluorescence at high resolution, deep inside living animals. By combining imaging chambers and photomarking of regions of interest in the tumor, single tumor cells can be tracked and visualized over several days. By using FRET IVM, dynamic changes in subcellular signaling events that are required for metastasis can be imaged over time, whereas multicolor IVM allows the visualization of cell–cell and cell–molecule interactions. These recent advances in intravital imaging open the field to new and exciting discoveries.
Important new insights into tumor cell biology
Recent IVM studies using the above-described techniques visualized how tumor cells exit the tumor and spread to a secondary site, generating new important insights into this process. For example, it is generally believed that cells at the tumor border have to invade the surrounding tissue, where they traverse physical barriers of the ECM and endothelial cells before they enter the circulation to be transported to a secondary site. Interestingly, IVM studies show a much more dynamic picture, in which cells switch between different invasion strategies to exit the primary tumor as a reaction to physical ECM barriers or intracellular signaling, as discussed below.
Hematogenous versus lymphatic tumor cell spread
Tumor cells can exit the primary site by entering either lymphatic vessels or blood vessels. Interestingly, IVM of lymphatic vessels, which drain labeled macromolecules, shows that lymphatic vessels inside hyperplastic lesions and the tumor mass are non-functional or even absent (Hagendoorn et al., 2006; Padera et al., 2002). By contrast, regions at the tumor margin contain functional lymphatic vessels and thus might be the starting point for lymphatic metastasis (Padera et al., 2002). Cells that metastasize through the lymphatic system will first be transported to the draining lymph node (Fig. 4A). From there, the tumor cells flow through efferent lymphatic vessels to the heart and into the blood system and, consequently, are transported to distant sites where they can form metastatic foci (Fig. 4A). Although the presence of tumor cells in regional lymph nodes is recognized as a prognostic factor for breast cancer and other cancers (Hartveit, 1979; Hartveit, 1984; Sleeman and Thiele, 2009; Tanis et al., 2001), it remains elusive whether tumor cells metastasize from the lymph nodes or whether their presence in lymph nodes merely reflects their intrinsic invasiveness (Das and Skobe, 2008). Therefore, it is unknown whether tumor cells use the lymphatic route, the hematogenous (blood) route, or indeed both, to metastasize to other organs.
To address this question, IVM has been used to visualize the signaling pathways that are required for the entry of tumor cells into lymphatic or blood vessels (Farina et al., 1998; Giampieri et al., 2009; Hagendoorn et al., 2006; Hoffman, 2009; Kedrin et al., 2008; Wyckoff et al., 2007). For this purpose, Gaimpieri and colleagues engineered MTLn3 mammary tumor cells to express Smad2 fused to GFP, which translocates to the nucleus in response to transforming growth factor β (TGFβ) (Giampieri et al., 2009), a well-studied inducer of tumor cell motility (Leivonen and Kähäri, 2007). Using high-resolution IVM of MTLn3 mammary tumors, they showed that TGFβ signaling is active in cells that move as single units, but not in those that move cohesively as cell chains. (Giampieri et al., 2009; Giampieri et al., 2010) (Fig. 4A). In addition, they showed that single moving cells enter the blood vessels, whereas collectively moving cells enter lymphatic vessels. As the motility of single cells entering the blood vessels is TGFβ dependent, blood entry, but not lymphatic entry, is blocked upon TGFβ inhibition (Giampieri et al., 2009). Interestingly, the number of tumor cells drained from the heart, which corresponds to the added effects of hematogenous and lymphatic spread (see merging point in Fig. 4A), also decreases upon inhibition of TGFβ signaling (Giampieri et al., 2009). This suggests that tumor cells in these tumor models use the hematogenous route to metastasize to other organs, as TGFβ signaling is only required for the entry of tumor cells into blood vessels.
These studies clearly illustrate that IVM of signaling pathways can provide crucial information regarding the spread of tumor cells, but future research is necessary to determine whether these observations are also true for other tumor models.
The role of proteolysis in invasion
The proteolytic activity of peptidases is important for several aspects of metastasis, including tumor growth, apoptosis, angiogenesis and invasion (Kessenbrock et al., 2010). The roles of peptidases, such as MMPs, include remodeling of the ECM and cleavage of numerous other substrates, such as growth-factor-binding proteins, cell adhesion molecules, receptor tyrosine kinases and other proteinases (Egeblad and Werb, 2002; Kessenbrock et al., 2010). Many of these roles are thought to be crucial for tumor cells to be able to overcome the physical barriers of dense ECM layers when they invade surrounding tissues and enter the blood (Christofori, 2006; Rowe and Weiss, 2008; Tanjore and Kalluri, 2006). These ECM layers include a three-dimensional fibrillar meshwork of interstitial tissues and the two-dimensional sheet of the basement membrane (BM), which underlies epithelial and endothelial tissues (Fig. 1) (Kalluri, 2003; LeBleu et al., 2007; Rowe and Weiss, 2009). The BM is thought to act as a particularly effective physical barrier owing to its small pore size (~50 nm). However, it is not clear whether the breakdown of these ECM layers is always required for tumor cell invasion and intravasation.
IVM experiments show that proteolytic activity supports the collective invasion of tumor cells (e.g. Friedl and Gilmour, 2009; Giampieri et al., 2009). For example, cancer-associated fibroblasts have been found to remodel the interstitial ECM meshwork, through both peptidase- and force-mediated matrix remodeling. This forms tracks through the matrix, which enable tumor cells to collectively move into the surrounding tissue (Gaggioli et al., 2007; Giampieri et al., 2009). Macrophages have been shown to assist in the entry of tumor cells into the circulation by producing chemokines and peptidases, leading to BM breakdown and tumor cell motility (Goswami et al., 2005; Wyckoff et al., 2007). Alternatively, tumor cells might degrade the endothelial BM themselves. In vitro, many tumor cells can form actin-rich structures, the so-called invadopodia, which locally degrade the ECM (Poincloux et al., 2009; Stylli et al., 2008). However, a corresponding function or even the very existence of such invadopodia in vivo has not yet been established. With the recent advances in high-resolution IVM techniques and protease probes, these questions might be answered soon.
Interestingly, using IVM, several laboratories have now established that ECM degradation is not always required for tumor cell invasion through dense ECM layers. For instance, Wolf et al. showed that, upon inhibition of MMPs, tumor cells can still invade connective collagen matrices by switching from a mysenchymal mode of migration to an amoeboid mode of migration (Wolf et al., 2003). Both in vitro and in vivo experiments have shown that mysenchymal migration is characterized by secretion of proteases and an elongated polarized cell morphology, whereas amoeboid movement is characterized by a non-polarized flexible round morphology of the cell and does not require any proteolytic activity (Wolf et al., 2003; Wolf et al., 2007; Wyckoff et al., 2007; Sahai and Marshall, 2003). The amoeboid mode of migration enables cells to squeeze through small gaps by forming bleb-like structures, thereby pushing these fibers out of the way without the requirement for proteolytic activity (Pinner and Sahai, 2008), allowing them to migrate within mammary tumors with high velocity (Wyckoff et al., 2006; Wyckoff et al., 2007). Although amoeboid movement does not depend on ECM layer breakdown, it might be facilitated by other peptidase-dependent processes, such as the activation of chemokines. Nevertheless, these data illustrate that a tumor cell can adapt its invasion strategy, that is, its mode of migration, to the surrounding microenvironment.
Invasion and intravasation within the tumor mass
In static histological samples, the tumor cells that are located in the surrounding tissue close to the invasive front of the tumor are identified as motile tumor cells. As the presence of these cells in the surrounding tissue correlates with poor prognosis, it is generally thought that tumor cells escape the tumor by invading the tumor stroma (Christofori, 2006; Rowe and Weiss, 2008; Tanjore and Kalluri, 2006). However, the contribution of motile cells within the tumor mass, which also enter the vessels, has either not been studied or simply been overlooked, as the motility and intravasation of this population of cells cannot be visualized in static histological images.
With the recent high-resolution IVM studies in mice, intratumoral motility and intravasation can now be directly visualized in real time (Fig. 5) (Farina et al., 1998; Giampieri et al., 2009; Kedrin et al., 2008; Wyckoff et al., 2000). In an elegant study using multicolor spinning disk microscopy, Egeblad and co-workers showed that stromal cells and leukocytes (including T cells, dendritic cells, myeloid cells and fibroblasts) exhibit higher motility at the tumor stroma of mammary tumors than within the tumor mass (Egeblad et al., 2008; Lohela and Werb, 2010). However, the motility of tumor cells has not been visualized in this study. The extent of intratumoral motility became clear when we tracked tumor cells through MIWs over long periods of time. By photomarking a subset of tumor cells, we observed intratumoral cell movement and entry into the vessels within the tumor mass (Fig. 3C) (Gligorijevic et al., 2009; Kedrin et al., 2008). Importantly, in contrast to tumor cells present in the tumor stroma, invading and intravasating tumor cells within the tumor mass no longer encounter physical barriers, such as the dense epithelial and endothelial BMs. Vessels that are embedded within the tumor mass, referred to as tumor-associated vessels, are less organized and less solid compared with normal vasculature (Fukumura et al., 2010; Jain, 1988). In fact, owing to the lack of an intact BM and irregularly organized endothelial cells and pericytes, these vessels have been shown to be leakier than vessels of healthy tissues (Baluk et al., 2003; Chang et al., 2000; Fukumura et al., 2010; Jain, 2003; Kessenbrock et al., 2010). Therefore, tumor-associated vessels within the tumor mass are more easily accessible to tumor cells, thereby alleviating the intravasation process (see cartoon in Fig. 5).
IVM also shows that, in addition to active entry of cells into tumor-associated blood vessels, tumor cells can also accidentally enter the blood stream when they are positioned close to a poorly structured tumor-associated vessel that collapses upon elevated interstitial fluid pressure caused by proliferating cancer cells. As a result of the collapse, big clumps of cells from the surrounding tissue shed into the lumen of the collapsed vessel and are transported through the vasculature (Baluk et al., 2003; Chang et al., 2000; Fukumura et al., 2010; Jain, 2003; Kessenbrock et al., 2010).
Taken together, IVM has shown that both active and inactive entry of tumor cells into the vasculature occurs within the tumor mass, implying that tumor cell migration and intravasation do not only take place at the invasive front. Therefore, intratumoral motility and intravasation should be considered an important escape route for tumor cells. This idea also explains the observation that many patients who have metastatic outgrowth show no BM breakdown and therefore no invasion at the invasive front at the primary tumor site; this was initially explained by a resynthesis of removed BM (Dingemans, 1988).
Conclusions and future perspectives
In this Commentary, we have focused on the advances in IVM and have given examples of its usefulness in studying the metastatic process. So far, most of the advances have resulted in the ability to image the earlier steps of metastasis, including migration, invasion and intravasation. Only a small number of studies have attempted to image cells in organs that are prone to metastasis, such as the lungs (Kimura et al., 2010), bone marrow (Sipkins et al., 2005), lymph nodes (Mempel et al., 2004) and spleen (Grayson et al., 2001). Unfortunately, these experiments rely on surgical dissection to expose the imaging site, which hampers long-term imaging and therefore the visualization of colonization and dormancy. The use of the cranial imaging window has overcome these problems for the brain (Kienast et al., 2010) and, to expand this advance to other organs, the next generation of imaging chambers should aim to visualize the spleen, liver and lymph nodes. IVM of colonization will help to answer many unresolved questions, such as why tumor cells colonize a specific organ without the formation of colonies in other organs, how long tumor cells stay dormant and what factors can reactivate them (e.g. angiogenesis, growth factors).
Although IVM has been successful in providing new insights into the early steps of metastasis, most studies are only observational. In the next few years, it will be important to move the field from observational IVM to experiments that can also characterize the underlying molecular processes. For example, new cancer models should be imaged that have been engineered to manipulate the behavior of cancer cells by inducible expression of oncogenes or signaling proteins (Le Dévédec et al., 2010). Developments in photo-manipulation will further help the transition to experimentally driven studies, such as laser-induced release and activation of caged compounds (NPE-caged cAMP) or of transcription. Moreover, high-resolution and FRET IVM of biosensors are important tools to study molecular processes, such as cell–cell communication, intracellular signaling events and the gain of traits that are required for cells to metastasize. For example, a FRET probe for the proto-oncogenic tyrosine kinase Src (Ting et al., 2001) can be used to study the regulation of integrin–cytoskeleton interactions during cell motility, and a reactive oxygen species (ROS)-sensitive FRET probe that responds to hypoxia (Guzy et al., 2005) might be important for studying the influence of the tumor microenvironment during escape and colonization. Unfortunately, most FRET biosensors have a low signal-to-noise ratio and are not sensitive enough to be used in IVM experiments. Biosensor development is an expanding field, and the next-generation probes should aim to increase the signal-to-noise ratio and to probe for proteolytic activity, proliferation, adhesion and senescence.
Given the increasing number of genetically engineered tumor cell lines and fluorescent reporter mice, the transcriptional and differentiation state of cells that metastasize can also be studied by IVM. For example, Pinner and colleagues engineered melanoma tumor cells to express GFP driven by the Brn-2 promoter, and showed that melanoma cells can switch from a less differentiated state to a differentiated state when these cells exit the primary tumor and enter the secondary site (Pinner and Sahai, 2008). These types of IVM experiments will be able to answer long-standing questions in the field, such as whether cells can transiently switch from an epithelial to a mesenchymal phenotype (EMT) during metastasis, or whether the metastasizing tumor cells have a stem cell phenotype.
As discussed in this Commentary, IVM is an important tool to gain knowledge of tumor cell dynamics during metastasis, which will enable the development of more powerful strategies for treatment of human cancer. Although xenograft cancer models in immune-deficient mice have been used extensively for IVM, these are end-stage tumors that do not recapitulate the natural development and morphology of common human cancers (e.g. Cardiff et al., 2000). More importantly, they lack particular interactions with the microenvironment, which contains key players, such as T cells, that influence tumor development and metastasis (Condeelis and Pollard, 2006; de Visser and Coussens, 2006; DeNardo et al., 2009; Orimo and Weinberg, 2006). Novel cancer models have recently been developed and result in mice that have a spectrum of tumors, which strongly resembles human tumor development and progression in the breast (Derksen et al., 2006; Jonkers et al., 2001; Olive et al., 2004; Xu et al., 2001), pancreas (Hingorani et al., 2003), lung (Jongsma et al., 2008; Meuwissen et al., 2003) and brain (Llaguno et al., 2008; Xiao et al., 2002). Even though the incorporation of fluorophores will be technically challenging, these models will undoubtedly advance the translational aspects of future intravital imaging experiments.
We thank Johan de Rooij, Onno Kranenburg and Stephan Huveneers for critically reading this manuscript, Tom Schonewille for technical assistance, and Anko de Graaff and the Hubrecht Imaging Center for imaging support. This work was supported by VIDI fellowships 91710330 (J.v.R. and E.B.) and 91796318 (P.W.B.D.) from the Dutch Organization of Scientific Research (NWO), a grant from the Dutch Cancer Society (KWF: HUBR 2009-4621) (L.R.) and an equipment grant (175.010.2007.00) from the Dutch Organization of Scientific Research (NWO). N.V. was supported by the intramural research program of NIAID, NIH. Deposited in PMC for release after 12 months.
↵* These authors contributed equally to this work
Supplementary material available online at http://jcs.biologists.org/lookup/suppl/doi:10.1242/jcs.072728/-/DC1
- © 2011.


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This project has been on my want-to-do list for some time. Organized by American Patchwork & Quilting, the 1 Million Pillowcase Challenge is a challenge for quilters, sewists, and crafters to reach a goal of donating one million pillowcases to charity. The counter is currently up to 522,000!
Annabel chose the main fabrics, and I selected the coordinating material for the bands. There are a variety of free patterns on the official web site, and I decided upon the one called, 'Roll it up!' It calls for 7/8 yard for the pillowcase body and 3/8 yard for the band. I liked it because it involves sewing the pillow with a 'burrito' method which leaves you with no exposed seams to unravel in the wash. If you've ever wanted to know how to sew a French seam, this is a great way to learn.
We made three pillowcases in one afternoon, one cotton and two flannel. It's a great project to do with kids and a way to familiarize young children with the how's and why's of sewing for charity.
Visit the web site here for additional information. Find the nearest participating shop that is collecting the pillowcases, the list of charities supported, and how to be sure your pillowcases will be added to the tally.
xo Karin
PS - If you've sewn for charity and would like to recommend a charity sewing project, please do so in the comments below!


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Museum of Contemporary Art Exhibit Opening: S, M, L, XL Go through four opening in this hands-on exhibit. As as visitor you can walk down a normal sized hallway that turns into an itty bitty … [Read more...]
New Exhibits Chicago Week of 5/25/15
Holiday Season 2012
Time to decorate the tree the with that shiny tinsel and holiday merry making! The Swedish American Museum's Brunk Children's Museum is one of the best decorated museums in Chicago. The Christmas … [Read more...]
Big Draw Chicago 2012
This year, this month, and all this week are events for Big Draw Chicago for some of Chicago's best museums! Here's the calendar listing! The listing is in alphabetical order. See if your favorite … [Read more...]
Museum Free Days and Discount Days 2012
Do you have a random day off from work or looking to take the kiddos somewhere? Look no further than your nearest museum free and discount days. Many of Chicago's museum are free to Illinois residents … [Read more...]
CMB's Museum Summer Drinking Special
Ever wonder where the closest watering hole is to your favorite museum? In this CMB special you will find out where the closest and best watering holes are including dive bars, jazz bars, and just … [Read more...]
Do you love astronaut ice cream, Bucky Balls, and Frank Lloyd Wright? Read Chicago's museums' favorite gift shop gear and stuff!
Are you are lover of the museum gift shop? Do you enjoy spending the last few minutes of a visit to a museum in the store? Well sometimes I find some of the best gear, jewelry, and stuff in the museum … [Read more...]
Holiday Wrap-up
This year the Chicago Museum Blog did so many Christmas and Holiday shows it is hard to begin and end the season of giving. Here are some highlights from the Shedd Aquarium's Holiday FantaSea Show, … [Read more...]
December's Holiday Museum Events!
O Christmas Tree, O Christmas Tree how bad I want to see thee! It seems that some homes in the Chicago area have already gotten the Christmas bug well so has the Chicago Museum Blog. Here you will … [Read more...]
Top 10 Chicago Museums [According to Facebook]
Can you find your favorite museum on this list? Most Chicagoans should be able too. One would probably think that the Field Museum is the favorite, but you would be wrong; it's the Art Institute of … [Read more...]
Museum Free Days for October 2011
Free is the place to be in October. With Autumn and cooler weather approaching kids and individuals will want some refuge from the chilly air.Head to Art Institute this month on Wednesday the 5th … [Read more...]


text: In order to create awareness on the Sexual Harassment of Women at Workplace (Prevention, Prohibition and Redressal) Act, 2013, an orientation programme was organized on 11.02.2019 at NESAC for all its employees including CISF, Research Scholars, Outsourced workers and Home guard volunteers in collaboration with North East Network (NEN). North East Network (NEN) is a women's rights organisation based at Shillong working on addressing gender issues through dialogue and dissemination. Shri P L N Raju, Director, NESAC graced the occasion as the Chief Guest. Around 128 participants attended the programme which was conducted in two sessions.


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How do you reduce your business travel expenses when you travel for work? Here at Davidson Village Inn, we have a few tips for saving money on business travel. Charlotte is home to many corporate headquarters including Bank of America and the east coast operations of Wells Fargo. And with Charlotte Douglas International being the 6th busiest airport in the United States, there are lots of business travelers coming and going through our area.
Here are 5 tips for minimizing your business travel expenses.
- You don't have to sacrifice to save
Get away from the hubbub of the Uptown area after a long day at work and escape to the small town of Davidson, North Carolina. Davidson is a quick 30-minute ride North from Charlotte. The location of our B&B, Davidson Village Inn, is not only convenient to the many nearby corporations, but is also in a safe, quiet part of town. Staying at a boutique inn such as ours can be a nice change from the chain hotels you might be accustomed to.
We offer affordable Executive Suites for business travelers complete with 4 poster Queen Bed, working desk and chair, flat-screen television, and a separate kitchenette area. We also offer specials throughout the year to help you save you money when you travel while also having a bit of fun. From our Pedal Around Davidson special to our Movie Night at Our Town Cinemas, you'll have a little something to do after a long day at work. Business travelers can also save money and keep their exercise routines on point when staying at Davidson Village Inn. When you stay with us while on business, you'll receive free passes to the Lake Norman YMCA. It is located 1 mile down the road from the Inn and offers lakefront activities, a pool, and state of the art equipment.
- Points aren't always the answer – explore your options
Many business people frequently stay at chain hotels when they travel simply because they get points for their stay. However, an addiction to earning points can prevent you from exploring other, better travel options. You may earn points staying at a chain hotel, but you could end up in a building near the busy interstate, without any personal service. Make sure to explore local lodging options when you travel: not only are these businesses just as affordable as chain hotels, they offer personalized service and are more familiar with the area. Not only that, they're frequently located away from busy highways so you can enjoy a quiet night's sleep.
- Eat healthily without breaking the bank
It can get pricey paying for every meal when you are away from home. Even breakfast can be a pain, and most hotel breakfasts leave much to be desired. Staying at an inn or bed and breakfast can offer better options. For example, when you stay with us at the Inn, we start each morning at 7:00 am with a complimentary breakfast buffet. The buffet is sure to start your morning off on the right foot. You'll enjoy a spread of fresh eggs, cereal, cheeses, yogurt, locally-made baked goods, local Summit coffee, fresh fruit, and juice. If you check in by 3:00 pm, we'll even treat you to an afternoon tea with cookies.
- Book direct to avoid extra fees
With online travel sites constantly promoting their amazing deals, it's easy to think that they're the best way to save when you travel. But don't forget – they're businesses, and they have to make money. They do this by tacking fees onto advertised rates. Also, because online travel agencies (OTAs) take a cut of the profits from any listing, many hotels and inns post higher rates on these sites, just to make up for the commission fees. So how can you avoid this? It's simple – book directly with the hotel or inn! By visiting the property's website or calling to book direct, you are guaranteed the lowest rate and will avoid any hidden fees. With smaller inns such as ours, you'll also be supporting a local business, not an online travel site!
- Getting around town.
When your travel brings you to the Charlotte area, there are quite a few inexpensive ways to get around. When you are in Uptown, look for the LYNX Blue Line Light Rail. The LYNX runs every 10 to 15 minutes depending on the time of day and is only $2.20 each way. There are 15 stations to catch the LYNX. There is also the Gold Rush Red Line Trolley cars that begin service as early as 6:40 am. The best thing about the Gold Rush Red Line is it's free!
Let us help you make your business travel a little less stressful. For information on our current specials or for assistance booking your room, please don't hesitate to give us a call at 704-892-8044, or book online. We welcome all travelers to the area on business or pleasure. We look forward to hosting you soon.


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- Crypto Research Report suggested Bitcoin will reach $19,000 in 2020 and $397,000 by 2030
- The researchers looked into Target Addressable Market as a key indicator for estimating a token's future price
- Cryptocurrencies are being traded more than they are being used for transactions or as store of value
New research suggested Bitcoin will spike to $397,000 by the year 2030 if it manages to penetrate at least 10% of its total addressable market.
Researchers behind the Crypto Research Report predicted a future valuation for Bitcoin, Ethereum, Litecoin, Bitcoin Cash and Stellar based on a number of variables and their penetration of addressable markets. Based on their estimates, Bitcoin will shoot to $19,044 in 2020, $341,000 in 2025, $397,727 in 2030, and $395,000 in 2033.
The researchers are expecting Bitcoin to increase its price by 107% before the year ends. By 2030, Bitcoin's price would have increased by 4,238%, based on the researchers' model.
The researchers predicted Ethereum to end 2020 with $331, a 45% increase from current prices. By 2025, they are expecting the world's second-largest cryptocurrency to increase by 1,463% to $3,549 and finally to $3,644 by 2030, a 1,600% increase taking into account the price today.
Stellar would boast the biggest price increase, from $0.06 today to $7.81 in 2030 and $8.26 in 2033, representing an almost 14,000% price increase in value.
The researchers looked into Target Addressable Market (TAM) as a key indicator for estimating a token's future price based on how they are able to penetrate those markets. TAMs include consumer loans, offshore accounts, reserve currency, store of value, online transactions, remittance, gaming, etc.
The research also found out cryptocurrencies' off-chain velocity has increased more than their on-chain velocity. On-chain velocity represents the movement of an asset in the blockchain while off-chain represents the movement of assets in the crypto exchanges. The research concluded cryptoassets are moving more inside exchanges versus them moving because of transactions (utility). Simply put, users are trading more than they are using cryptocurrency for their supposed intended use, such as a medium of transaction or as a store of value. This is true for Bitcoin as well as the other coins reported in the research.
The Crypto Research Report's Bitcoin price forecast coincided with Bloomberg's Crypto Market Report, which predicted Bitcoin will revisit $20,000 before the year ends. Bloomberg looked at Bitcoin's correlation with the stock market and the COVID-19 pandemic as signs that the world's first cryptocurrency is maturing into a proper asset class.


text: KILLERWAXX Pink Water Spot Remover 470ml KILLERWAXX Pink Water Spot Remover 470ml KILLERWAXX Pink Water Spot Remover 470ml KILLERWAXX Pink Water Spot Remover 470ml KILLERWAXX Pink Water Spot Remover 470ml £9.99 Quantity: at £9.99 each Add To Basket 16 in stock. KILLERWAXX Pink Water Spot Remover has been developed to remove hard water spots on painted surfaces and glass and has low concentrations of acids and speciality materials. Water spots may form on painted surfaces when hard tap water is used to rinse off after washing and is then left to dry off naturally. These water spots comprise of calcium carbonate, or limescale as it is more commonly known, and other evaporative salts. If such spots are left on painted surfaces for any significant length of time, they can harden to the point where they become very difficult to remove, even by machine! In extreme cases, such deposits can attack underlying surfaces, causing etching. Etching refers to chemical erosion of the uppermost layer of paint (more often than not a clearcoat), and is also caused by the corrosive effects of strong organic acids in bird droppings and bug remains. Such contaminants should always be removed as quickly as possible in order to lower the risk of etching occurring. DIRECTIONS: Follow directions and precaution completely. Rinse vehicle with water to ensure surface is cool and to remove any dirt. Dilute product with water to achieve desired strength. Using a sponge or cloth with raw metals apply product to area needing treatment. Working an area, no larger than 3' x 3', rub product for 15 to 60 seconds. Do not allow product to dry or run on surface. Inspect area thoroughly to ensure water spots have been removed. If necessary, repeat previous step. Upon complete of the water spot removal process, wash surface completely with KILLERWAXX FOAMZILLA car soap to ensure all product is free from the surface. *Be one of the first to use KILLERWAXX anywhere outside of the United States of America!!


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Find something for everyone on your list! Alpine is home to fabulous boutiques, unique finds, and great designer brands.
With a collection described as "country-boho vixen" Bella's Closet, offers trendy women's clothing and accessories. Hand made dream-catchers are a signature item at Chic Boutique alongside boots, belts, and jewelry. An assortment of custom made articles are available at KC's Closet. Find something fabulous for your four-legged family members at Ruff Life Dog Boutique with everything from leashes to toys to apparel. Knick-knacks, home decor, vinyl records, and unique treasures are all a staple at Back in Time Vintage Emporium. Adventure seekers and cycling enthusiasts can get outfitted at Alpine Ride Shop. For those looking to spruce up their garden, Alpine Garden & Gifts will delight with their selection of native plants, succulents, and yard art. For the sportsman in your life stop-by Right to Bear for all your hunting needs. To ensure all your gifts and cards arrive on time, swing by Postal Annex, who also has a great assortment of gifts.


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ManaSurge Presents: Wonderland Dreams Set Review
ManaSurge Presents: Shadowverse Wonderland Dreams Set Review! Come check out our podcast and hear what SunFireX, Bjorn, JaZe, iseestars and GlassFists have to say with regards to their first impression of the cards before the release! What card are you most excited for and is your favourite?


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Automotive Product Finder Magazine | We hope for more business in commercial vehicles post Feb 2020
We hope for more business in commercial vehicles post Feb 2020
Automotive slowdown to have short-term effect on metalworking
2W segment is ramping up production faster than 4W: Anurag Garg
Cover Story
...says Joy Haria, Head - Marketing and New Business, Patel Auto Engineering Works, while sharing his views on the effects of BS VI regulation, slowdown and export trends.
What is the current status of the auto component industry?
The overall production of automakers across categories have seen year-on-year decline, this fiscal. The vehicle production is down. For suppliers, the double digit decline is experienced primarily more in profits than the revenue. Given the slowdown in the domestic market, we have decided to rethink over our reduced future target. The exports have started to see decline, thus the focus inclines towards exports and we are trying to push 50 per cent of the volume through exports to developing nations, where market conditions are similar.
What are the recent trends in component manufacturing,?
We have received orders from many electric vehicle (EV) manufacturers in India. Steel is the major component used in developing the body while other parts are built by aluminium. E-rickshaw manufacturers are booming in India. Components used in the EVs like two-wheelers and three-wheelers are strong on demand. E-rickshaws are comparatively faster. Use of alloys will be more significant with reference to weight. Other trends in the segment are that of carbon fibre. Some components are manufactured using carbon fibre. Steel use will be very well in EVs.
With upcoming BS VI regulations, have you received new business?
We are working closely with many automakers in India. We are currently manufacturing emission control system for one of the established brand of trucks and busses. We have received the design and it was finalised some three months back. We also are in talks with others for similar systems. We hope for more business in commercial vehicles post February 2020. Till then there will be lot of trials and testing.
What is your R&D team currently working on?
We are working with one of the startup to develop tires for e-rickshaws first and then for other electric vehicles. It is a challenge as there are many established tire-makers. Our product will serve as a robust alternative to the tires available in the market. The usp of these tires will be light weight.
What are your exports plans?
One of the major issue here is do manufacturers have their plants built following global standards. If not, it is a high-time to do so. Being a manufacturer, your job dimensions are limitless. We will soon start manufacturing components for European and Bangadeshi automakers. They visited our state-of-the-art manufacturing facility to validate and to inspect our plant. We have recently a provisional go ahead. These manufacturing contracts will be on the new EV segment, car components and e-rickshaws.
Our plans are to build electronics portfolio. Use of electronics will see a growth by 2022. EVs will bring more demand and we will not miss this growth trend in the market. Automakers previously were not that open for taking into consideration a new supplier. With changing market and new technology, situation has changed.
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The best way to spy on someone is doing it without his/her knowledge. This is because people change their behavior when they know they are being followed or monitored. The same case happens with kids. In fact, they become very nervous and might even stop some activities altogether to keep their privacy. This brings us to the question; how can one monitor a phone without jailbreaking? The following is a review of several apps that allows you to monitor various phones without jailbreaking them.
#1: Highster Mobile Spy
This is the best cell phone spy software developed by ILF Mobile Corp to help people track targeted gadgets like mobile phones and tablets. Unlike others, this app is very advanced and uses the same technology that security authorities utilize to gather information from various sources. While other software requires you to have physical access to the phone being spied, Highster Mobile Spy will get all the information you want remotely. You only need to get the number of the target phone, and everything will be ready to go. Once you add the smartphone to the app, the software accesses all the details in including text messages, social media messages, and live chats. You can even get the messages that were deleted by your cautious teen. The software will also allow you to track the phone using GPS tracking technology, take pictures remotely, learn how to receive text messages from another phone, and even geo-fence the kid. With this software, you no longer have to worry about your teen boy or girl ever knowing you are monitoring, everything is hidden from them.
#2: mSpy
mSpy is another great option when you want to monitor your teens without jailbreaking their phones. Though mSpy is not as advanced as Highster Mobile Spy, you can monitor the kid's phone by entering its details in the mSpy dashboard on your smartphone or computer. It gives you access to all messages, online chats, call logs and web browser history. You also get full access to stored numbers and photos. However, to get full access and control of the target phone, jailbreaking the phone is necessary.
#3: Mobistealth
This cell phone tracker android monitoring app is very effective in tracking your kid and all that he/she does. To install it, you do not have to jailbreak the target phone. You add the details of the target phone to the Mobistealth online account activate to start monitoring. The app allows the parent to access SMS history, web history, line chats, contacts, saved images and call logs. It allows you to locate the kid using GPS tracking system. The only shortcoming of this software is the lack of in-person customer support.
#4: XnSpy
XnSpy is one of the fastest upcoming software that makes it easy to access your kid's phone without having physical access to it. You only need to know the model and number of the target phone to get all that is happening. You can access call records, text messages, pictures and video galleries, and real-time locations. Though the app is relatively cheap, what you can do to the target phone is very limited. For parents who would want to block some online addresses, calls, or text messages, more advanced software such as Highster Mobile Spy might be necessary.
If jailbreaking your child's phone to install spy applications is difficult or will arouse some suspicion, the best thing is using any of the above monitoring apps. They allow you to get all information you need from a child so you can take the right measures to stop harmful activities, keep the kid away from bullies, and protect them. Make sure to understand to what extents you want to go in order to pick the best software for installation without jailbreaking the target phone.


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Isrever is a game involving abstract strategy and played on a board with 8 rows and 8 columns and a set of distinct pieces for each side. The pieces are disks with a light and a dark face, each side belonging to one player. The player's goal is to have a majority of their colored pieces showing at the end of the game, turning over as many of their opponent's pieces as possible.
★ Both one and two player modes.
★ Fun AI with 4 levels (beginner, medium, hard and expert).
★ 8x8 or 10x10 game boards to choose from.
★ 3 great themes (Classic, Candy Cane Twist and Pop Top Twist) - each with 2 board styles.
★ Great background music and sounds.
★ Instructions for play are accessible from game screen - click on (?) button.
Thanks for buying Isrever Twist!


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Before registering – if you live in one of the following countries, and are registering for a course in the U.S., please call the number provided. Otherwise, please follow the online registration process in the table below.
Singapore/Malaysia, contact Jacqueline Kee at 65-6846-1518
China, contact Celia He at 86-028-62016816
France, contact Pascal Tournier at +33 6 27 48 01 68
Germany, contact Frank Marschalek at +49 6188 4486231
UK/Ireland, contact Wendy Rowland at 01733 232211
For questions about registering for courses in:
The United States, contact Barb Shrum at 231-933-8162
Mexico, contact Esmeralda Rivera at 52-614-4242281


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We had given you a primer about the changes in the process and the examination for CAT 2014 in this article. Apart from procedural changes like exclusively online purchase of vouchers, and more important ones like the reduction in the number of time slots, there are also several changes in the CAT 2014 paper pattern that have already been spelled out. Some of these include no sectional time limits, increase in the duration of the exam, and increase in the number of questions.
A lot of these changes would have created significant anxiety among all MBA aspirants. In our continuing quest to provide the very best guidance to our readers, we are organizing a Live Chat with ARKS Srinivas on Wednesday 27th August 2014 at 9:00 pm.
This Live Chat is aimed at clearing all your doubts pertaining to CAT 2014, in view of the new Paper Pattern. Ask ARKS about adjustments to test taking strategies, which sections should you focus on, and about the trade-off between accuracy and attempts - this is going to be a critical aspect in your test-taking strategy. You can also ask him from tips for verbal wizardry to questions on quantitative conundrums. This is the third in our series of Live Chats for CAT, and we advise you to make the best use of it. (Read the previous LiveChat with ARKS Srinivas here and the abridged transcripts here. Our earlier LiveChat with Hemang Panchmatia is available here and the abridged transcripts are available here).
What you need to participate in this LIVE Chat -
1- Queries on CAT 2014 (the new paper pattern, test taking strategies etc.)
2- General Queries on Verbal, Quant, for CAT, XAT, GMAT etc
3- An account on InsideIIM. Register here, if you don't have it already
4- A good net connection
5- and Patience, all your Queries will get answered for sure :).
The live chat will take place at InsideIIM LIVE.
About ARKS Srinivas
ARKS is a household name in test-prep circles. He is the CEO of VistaMind Education and he is a director of Vanguard Business School. In his avatar as test-prep coach with VistaMind and earlier with T.I.M.E, he has safely guided countless students into their dream B schools. He is an alumnus of IIM Calcutta.
You can read some of his earlier articles on InsideIIM.
The CAT Verbal Enigma – Part One (also read Part Two and Part Three)
All those preparing for top business school exams in India – CAT, XAT,IIFT and GMAT should follow our exclusive aspirant dedicated section – Final Admit here. It is your destination before reaching your ultimate destination- in a Top School in India. Most of our users score 75 percentile and above. Most of our users aim for the best schools in India and the world. Our users collaborate and solve questions together here. Use our test prep platform to get the extra edge.


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You are using a version of browser which will not be supported after 27 May 2018. To continue to transact with MOM securely, please follow these steps to enable the Transport Layer Security (TLS) of your web browser, or upgrade to the latest version of your browser.
You must have the same CEI certification as your EA's licence type, regardless of your area of specialisation within your EA.
Find out more about the different CEI certifications.


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We're back in town, leaving holidays behind to catch up again with our dear places. Every single day has a worth-telling story, but nothing compares to the Fall Rockers chronicle: join the crib and set upon Autumn with Graffitishop.
When summer is over, it's time to shine on the main stage in town: your square. That's why Fall Rockers designed their outfits to enhance every tone around the city, going much further than anyone else.
Matched sneakers, loose sweats, T-shirts, light jackets: simple surviving rules for breaking out from the pack and turning the city mode on. Fall is coming, start rockin'!


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Mouthpiece orifice
Discharge coefficient much closer to 1 can be obtained using a mouthpiece orifice (Figure 2.37) so as to obtain a more regular outflow; the construction is obviously more complex. The instrument is more expensive than the plate orifice and is therefore more suitable for a permanent installation. The corresponding discharge coefficients are between 0.96 and 0.99 for 0.3 < A1jA1 < 0.8 and for Reynolds numbers above 104.
22.214.171.124 Venturi tube
The outflow through the plate orifice (to a lesser extent through the mouthpiece) is accompanied by significant losses generated in the vortex produced by the sudden contraction regions, so it is more efficient to use a Venturi tube (Figure 2.38), which is made of a convergent, a constant section tube and a diffuser with a small angle of divergence; the losses of stagnation pressure slightly exceed those that are generated in a tube with a constant section of equal length. The device is rather long and expensive, it is used in permanent installations in power stations and chemical plants.
The static pressure is measured at the entrance of the convergent and in the throat, the discharge coefficient is approximately 0.995.
It must be emphasized here that the quoted values of C are valid only for a well-defined geometry and thus are only a guide for different geometries. In ISO regulations, from where the previous figures are taken,
Figure 2.37
detailed plans of the various types of flowmeters, the rules to be followed when installing and measuring, quality of workmanship and materials to be used are given.


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Scrabble & Words with Friends Word Unscrambler
Definition of Aquanaut
2 Definitions of Aquanaut
The definition of aquanaut, the meaning of word Aquanaut
Is aquanaut a scrabble word? Yes!
- n.- an underwater swimmer equipped with a face mask and foot fins and either a snorkel or an air cylinder
- n.- a skilled worker who can live in underwater installations and participate in scientific research
2 Synonyms of Aquanaut
Aquanaut is worth 17 points in Words with Friends and 20 points in Words with Friends
There are 8 letters in aquanaut: A A A N Q T U U


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They mostly all started out by just browsing our web site before deciding to make the decision to become a performer.
You can even do the same yourself - it takes just a minute to register and verify your identity and then you're ready to start broadcasting your naughtiest, most intimate moments with like minded individuals over the internet.
Our system have been connecting people right in their calling area for over 3 decades 24/7 There are 100`s of people, men & women on line on every given hour speaking English or Spanish and EAGER to chat with you .
To use the site's full features, you need to allow viewing Flash content in your browser.
They've all got their own various kinks too so whatever sort of fetishes you might have, we're bound to have a live cam right up your alley. We hate places pulling the old bait and switch move just as much as you do, so you'll never have to worry about us hassling you for a credit card number.
We couldn't be happier to offer all of this to your 100% free of charge.
However, please keep in mind that the HTML-5 site version has substantially limited features (non-optimal video quality possibility of delayed streaming).
We recommend that all our users opt for the Flash-version of the chat (currently in use). To become a premium (GOLD) member for life and unlock this feature, all you have to do is buy any amount of tokens one time!
If you are unhappy with the way Flash Player is functioning on your computer, please try switching to the HTML-5 site version by pressing on the "rocker".
We won't blast you with many emails, just a few when telling you about us is important.
Adult is the web's finest live web cam site, featuring nothing but the most beautiful ladies as well as couples sucking, fucking, and doing just about anything you can think of, live in front of their web cams.
It's a great way to make some easy money and have a good time while you're doing it.
While we have countless ladies and couples broadcasting live on our website, it's by no means all we have to offer.


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How much do you love AMC's The Walking Dead? Do you find yourself imagining what it would be like to be on the show, trying to survive the zombie apocalypse alongside the remaining survivors? Do you care so much about certain characters that you become physically ill when they are killed? Are you so obsessed that you would consider taking a vacation to a private Bahamian island with some of the cast and crew?
If you answered yes to that last oddly specific question, you're in luck, because that exact opportunity is available to you next weekend, right at PortMiami.
Multiple cast members from the popular TV series The Walking Dead will unite for the second-annual Walker Stalker fan cruise on the Norwegian Pearl February 3 through 6. Cast members scheduled to be onboard
According to Luke Blackburn, who describes himself as a "warrior" with Sixthman, the group assembling the Walker Stalker cruise, this year's version has a lot in store for those eager to rub elbows with the series' stars.
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"On the first
As with anything last-minute, you'll have to pay a pretty penny to lock down one of the last spots on the ship. Currently, the ship's limited vacancy starts at $815 per person (based on double occupancy). Fans dying to pay the price it takes to get onboard with The Walking Dead crew will find themselves in the middle of more than just a Comic-Con experience; they'll actually be on vacation with the series' stars.
"The celebrity guests are having just as much fun as our cruisers, and they want to experience all the event has to offer," Blackburn says. "Each celebrity guest is involved with a panel, autograph session, and photo ops, and a few of them are hosting special onboard experiences."
Walker Stalker Cruise
February 3 through 6, departing from PortMiami, 1015 N. America Way, Miami; miamidade.gov/portmiami. Visit walkerstalkercruise.com.


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The Division for Agricultural Policy Analysis, your knowledge provider within the Agriculture and Fisheries Policy Area. Activity Report 2009
Dirk Van Gijseghem & Tom Van Bogaert (eds.)
February 2010
With this activity report we want to provide an overview of the studies, reports and data gathering carried out by the Division for Agricultural Policy Analysis (AMS) in 2009. Besides giving a short summary of the content of the studies and reports, we will look at how the recommendations found their way into the policy. The Division for Agricultural Policy Analysis considers it its task to analyse agriculture within its changing context, inform the Agriculture and Fisheries Policy Area about these changes and proffer advice with a view to launching new initiatives.
The number one provider…
As a policy advisory service AMS works for the Minister of Agriculture and the divisions of the Agriculture and Fisheries Policy Area. For certain assignments it also works together with colleagues from other policy areas, or with external collaborators (consultancies, research institutes). The division also has a number of coordination tasks, such as the supervision of the strategic policy cycle. In 2009 the division coordinated the drafting of the contribution from the policy area to the coalition agreement for the new Flemish Government, which was sworn in in July.
... of knowledge ...
AMS carries out knowledge-intensive assignments following requests from other units within the Agriculture and Fisheries Policy Area. Each year, a survey is held in order to draw up an inventory of new assignments. According to the available competences and manpower the division carries out the assignments.
While it does so, AMS ensures that the knowledge it makes available is high-quality knowledge. By this we mean that the knowledge is valid, reliable, accurate and up-to-date. The knowledge is understandable and adapted to the conditions required by the client. Assignments are carried out in the form of projects, so that the client has a clear view of the use of manpower. Finally, the division takes care that the assignments are carried out in a coordinated way. Attention is given to collaboration between different units, both within and outside the policy area.
The division is also the knowledge pool of the policy area. When there is a need within the policy area for knowledge to solve a certain policy problem, the knowledge of the division is consulted.
The division, as chairperson, is responsible for the Platform for Agricultural Research. The Platform unites the Flemish Government, the faculties of agriculture of the various universities, the research institutes, the polytechnics and the test centres. This collaboration is to contribute to a good flow of knowledge and skills between research, practice and the agricultural sector. The White Paper on Agricultural Research, which was presented in March 2009, constitutes the knowledge agenda for Flemish agricultural research in the coming years.
... and information ...
Every year, AMS gathers quite a bit of information about the Flemish agricultural and horticultural businesses. This information is either gathered by the division itself, via the Farm Accountancy Data Network, or delivered by third parties, such as the Agency for Agriculture and Fisheries of the Federal Public Service Economy. The information is analysed, interpreted and explained in the different reports that are drawn up by the department. The Agriculture report 2008, presented at Agriflanders, and Pocket agricultural indicators, presented at Agribex, have become a reference when it comes to Flemish agriculture and horticulture.
... also in the future
In 2010 the division will celebrate its fifth anniversary. Therefore, it was good to look back in 2009 to what we have achieved and at what improvements we can make in the coming years. That is why a new division plan has been drawn up. This will serve as a guide for the further expansion and operation of the Division for Agricultural Policy Analysis in the period 2010-2014.
AMS provides knowledge and information to support the Agriculture and Fisheries Policy Area and to stimulate it to pursue a sustainable, future-oriented agricultural, fisheries and rural policy.
This mission is accomplished via 5 core tasks:
1. AMS carries out multidisciplinary study activities based on its own AMN data or secondary data collections in order to better understand and explain developments. AMS looks for relationships across areas, describes regional and (inter)national trends, uses models and explores possible future developments.
2. AMS guarantees a continuous flow of high-quality data, information and knowledge. To this end, AMS develops various instruments in the area of data production, publication and management.
3. AMS uses indicators which signal developments in agriculture to the AF Policy Area and the Flemish authorities and monitor the results and effects of the agricultural policy.
4. AMS, on its own initiative or on request, makes its expertise available to the departments of the Ministry of Agriculture and Fisheries and the Minister.
5. AMS translates the results of agricultural research into policy recommendations, and vice versa, and coordinates the Platform for Agricultural Research.
The main objectives in order to fulfil these core tasks are:
- a high-quality service
- efficient, high-quality data gathering
- optimal valorisation of information and knowledge
- efficient and productive internal processes
- competent and happy employees
- innovative thinking and working
- development of partnerships
In 2010 we will put all our efforts into realising these objectives.
Original version:
Van Gijseghem D. & Van Bogaert T. (reds.) (2010)
Afdeling Monitoring en Studie, uw kennisleverancier binnen het beleidsdomein Landbouw en Visserij, Activiteitenverslag 2009
Departement Landbouw en Visserij, Brussel.
Departement Landbouw en Visserij
Ellipsgebouw (6de verdieping) - Koning Albert II-Laan 35, bus 40 - 1030 Brussel
Tel. 02 552 78 20 - Fax 02 552 78 21
E-mail: firstname.lastname@example.com


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Description:Used Ketema Tolhurst 48" X 24" Batch-O-Matic T-316L S/S perforated basket centrifuge with hastelloy clad housing. Rated 73#/ cu. ft. material. Dual acting hydraulic unloader. Bottom discharge 12.8 cu. ft. maximum @ 7" cake thickness. Driven by 30 HP 1200 RPM high efficiency motor with oil gear hydraulic drive giving basket speed 0-1080 RPM. Built in 1990. S/N 56600-4 . FOB: Wilmington, NC
Location: Stock located in Wilmingon, NC
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Ariel Winter Carries a Fendi Monster Backpack
It's truly next-level.
Another day, another amazing back-to-school look. This time, Ariel Winter is giving us major first-day-of-class inspo in an orange, furry Fendi bag. While hers costs over $2,800, you can definitely find a similar backpack on a budget. And the best part? The monster decal on the front, and the furry charm at the top add tons of playful personality. This backpack will definitely make you stand out in the classroom in the best way possible. If you're ready to take your school style to an insane new level, click through our roundup of affordable backpacks with the coolest design details.


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This potato and sweet potato puree with vegan chipotle queso is completely different from the classic version. Still delicious though.
At this time of year, at Christmas and Thanksgiving, mashed potatoes are a must on our table. Well, I'll tell you that in my house it is very common throughout the year. The truth is that we love them and we eat potatoes and sweet potatoes pretty often. This puree is one of our favorites, and the best part of this potato and sweet potato puree is that I can use the leftovers to make tacos or "tostadas".
I made this recipe for Thanksgiving one year and it was delicious, so it's been in our table since then. It's a classic and super simple recipe of potato and sweet potato puree but with a twist. The potatoes get cooked in salty water. In the end, then mashed with crushed black pepper, salt, and butter (I use vegan butter).
This vegan chipotle queso is the best, and it's super easy to make, you only need three ingredients (four if you count salt as one). You can use it to make nachos, or roasted broccoli or anything else that comes to mind. Just drizzle it on top of anything for an amazing kick of flavor and creaminess.
So just before serving this potato and sweet potato puree, I add the vegan chipotle queso. And if you want a little bit more flavor, add some chopped cilantro…… it really adds an amazing finishing touch. This is a Mexicanized puree. ? Don't you think?????? Now let me share with you the recipe for this
potato and sweet potato puree with vegan chipotle queso,
my take on mashed potatoes. If you like it, please share. Also, don't forget to rate it with five stars down in the comment section, take a picture, on Instagram with the #piloncilloyvainilla or on Facebook and Pinterest. you have no idea how much joy seeing you recreate my recipes brings to me. I also learn a lot from all of the changes that you make to them.
Potato and sweet potato puree with vegan chipotle queso
- 2 large potatoes cut into pieces
- 2 large sweet potatoes cut into pieces
- 2 teaspoon of salt
- 1/4 teaspoon pepper
- 3 tablespoons of butter vegan butter
- 1/2 cup to 3/4 cup of water from which the potatoes were cooked or almond milk or rice milk
Chipotle vegan cheese
- 1 cup unsalted and unroasted cashews raw
- 1 pinch of salt
- 1 tablespoon of chipotle chile in adobo
- 1/2 teaspoon garlic powder
- Cover the pieces of potato and sweet potato with enough water and a teaspoon of salt. Leave covered until fully cooked.
- When fully cooked, drain them the water (saving about a cup of water), mash them and mix with the rest of the ingredients.
- Blend the cashews with the garlic powder, the chipotle chili pepper in adobo, the pinch of salt and half a cup of water. Blend until creamy.
- Serve the vegan chipotle queso on top of the puree.
- Use leftovers to make crispy tacos or tostadas.


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Serving Apache Junction and the East Valley
Trailer Repair & Manufacturing
When it's time to have your timing belt replaced, get in touch with E & C Mobile Mechanic for immediate and accurate replacement or repair services.
Water pumps driven by timing belts
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