text:
New improved design - Today's low price!
NEW! We've added a unique gel pad to our Bio-Lock heel cup for advanced cushioning and shock absorption. So, you get cushioning where you need it and firmness where you need it!
NEW! Reflexology massage insert!
Realigns your spine and pelvic area to reduce knee, hip and lower back pain.
Independent clinical study showed WalkFit® technology relieved foot pain in 90% of users.
NEW WalkFit Platinum Orthotics are guaranteed to get you back on your feet - pain free!
Over 25 million men and women, throughout the world, have used WalkFit® orthotics to walk, play and work without pain. WalkFit® Platinum takes comfort and stability to the next level with extra cushioning and odor protection. See how the world's #1 custom-fitted orthotic can improve your life. Try WalkFit® Platinum today -RISK FREE!
Realign your feet, realign your life.
The benefits of WalkFit® orthotics:
- 1Aligns back and shoulders
- 2Relieves lower back pain
- 3Balances hips for easier movement
- 4Reduces stress on knee joints
- 5Cradles feet for improved stability
Cups foot with durable support to relieve discomfort! Distributes your weight evenly across your foot to help prevent collapsing, pressure points, stress and rubbing. This reduces painful burning, bunions, corns and calluses.
Eases impact by evenly distributing the force of each step! Flexes and cushions to help absorb destructive shock waves from traveling up through your entire body every time your foot hits the ground!
NEW! Cushions your heel for ultimate comfort! A cushioning gel pad in the heel provides extra shock absorption and comfort.
No other orthotic offers you this custom comfort feature! 3 sets of customizable arch inserts allow you to decide which feels perfect for you.
Clinically Tested
Over 25 Million People have Enjoyed Relief
with WalkFit Orthotics
New research reveals hidden cause of joint pain
Researchers have now discovered that years of wear and tear from body misalignment causes damage to the delicate bones and tissues in your joints. But by addressing the cause from the inside, you can reduce inflammation and pain, and even support the growth of healthy cartilage in worn-away joints to give you more flexibility and mobility.
Joint Support corrects hidden causes of pain so your body can repair itself from the inside and out
Customer reviews
Stephen S. — "I walk with my wife a lot, but I have trouble keeping up with her and I try and play catch-up the whole time. It doesn't always work. Now it does. The pain is gone in my knee. I'm losing weight. I can't see myself walking without these things. One day, I had pain in my feet, pain in my knees. The next day, I had no pain at all! And the only difference was putting them in my shoes. I found the solution to my pain problems and I'm going to keep using them."
Mike K. — "The best thing, no doubt about it, is that I'm able to be a grandpa again. The WalkFits® really are a blessing. I was laid up for six months. My ankles would give out on me. But I have not fallen, tripped or had a problem since I've had these. 100% gratifying. I'm a very happy guy and a very lucky guy."
Jim M. — "I've been wearing these for about three years now. I got a pair in my work boots and a pair in the shoes I wear in the house all day. Every pair of shoes I have has them. If I don't wear them then my feet ache again."
Shem W. — "I tore the ACL in my left knee and then I tore the one in my right knee. And then after that I said, That's it. I'm not going to play anymore. WalkFit® has changed my life. I never thought they could do what they do for me. It's just amazing. I do not leave the house without my WalkFits®."
Cathy M. — "I've done everything to get out of pain. I've had steroid injections at the site and I've had custom-made orthotics, and I still could NOT walk!! The WalkFits® made a huge difference. Not to have the pain was like the clouds parted and the sun came out. To be able to do those things that I thought I'd never be able to do again, that gives me joy means a lot to me."
Paul R. — "Years of firefighting did take a toll on my feet, and nothing helped till I found the WalkFits®. They feel great. There's no pain. I kick myself for spending over $200 on those other orthotics when I could have bought 10 WalkFits® for the price of one. I've been wearing them for five years and haven't had a lick of pain ever since. They feel great! There's no pain!"
Vetalyn S. — "Since I purchased these in October 2009, it's the best my feet ever felt for as long as I can remember. Now I can walk for miles without any burning, pain or having to take off my shoes after wearing them for a short period."
Ralph G. — "My cushions thus far are comfortable and seem to be quite helpful."
Janet — I've had back problems for years. WalkFit® Platinum has greatly reduced my pain and now I can walk wherever I want to!
Douglas — Being a police officer, and being on my feet all day, I can't tell you what a relief it is to have these WalkFit® Platinum insoles in my shoes. I just wish I had gotten them sooner.
Frances — I am a registered nurse who has been working since 1969. I do 12-hour shifts. Thank you! My feet are HAPPY! These are great!
Heather — I believe that your orthotics are the best fitting and the best price!
Rosemarie — God bless your shoe inserts. I saw an infomercial about a year or two ago and figured I'd try it out. Here I am all this time later and I won't wear shoes without it. It's affordable, which is great b/c I don't have hundreds to pay for custom orthotics, and it's a great help and the cheap store-bought inserts can't even compare. I don't know how I ever lived without them. Please never stop making these. I just bought 4 more pairs just in case you do though! (but please don't lol)
Watch the TV Commercial
Money-Back Guarantee!
Try WalkFit® for a full 30 days. If you're not completely satisfied, simply return the product for a full refund of the purchase price (less shipping & processing).
Here's what some of the over
25 million users say about
WalkFit Orthotics:
"I felt relief almost immediately. My feet and back feel better. I wear them in my shoes all the time."
— James D.
Philadelphia, PA
"I put them in my shoes. They worked right away. I love 'em. They made the pain go away."
— Janice M.
Midland, TX
Doctor recommended
Dr. Allen Selner,
Doctor of Podiatric Medicine and expert in foot biomechanics
"Your feet are the foundation of your entire body. WalkFit® orthotics help to realign the heel and ankle. You're putting your entire body in better balance with stability and comfort."


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For years, government agencies have been reporting on the growing girth of Americans. For years, the media has been focusing on these numbers and how they are affecting longevity, medical costs and productivity in the work force. For years, our industry has tried to get the word out that exercise and good nutrition are necessary to combat this epidemic.
So why was I surprised to read recently that the obesity rate has stalled? The rates for women and children have held steady for 10 years, and for men, the rate has held steady for five years. Does that mean that the government, the media and our industry have finally been heard and that people are responding?
I'm not quite ready to jump up and down for joy just yet, especially since one-third of adults still are classified as obese. I'll be much happier when I see an actual decrease in the obesity numbers, but I guess you have to start with a plateau.
What do we need to reverse it? Dr. William H. Dietz, director of the division of nutrition, physical activity and obesity at the Centers for Disease Control and Prevention, told The New York Times that the data are promising, but to reverse the trend, we need policy changes or environmental changes. Some experts suggest that, like the push to decrease smoking, the only way to lower the obesity rate is to implement new policies that penalize those who engage in unhealthy behaviors or offer incentives related to exercise and food, possibly through taxation or bans on advertising of certain foods.
For some people, the idea of the government getting involved in taxing goods or banning items related to people's personal choices is deplorable. If someone wants to risk getting cancer by smoking or risk dying of obesity-related diseases, then that is their choice. The government should stay out of it.
The problem is, the government already is involved in this — as are you and I. We end up paying for the increased medical problems of these individuals. It costs us in higher insurance premiums or higher taxes as the government picks up the tab. Why not penalize people who engage in risky behaviors rather than penalize those who choose to engage in healthy behaviors?
If it takes higher taxes on junk food, then I'm all for it. However, that doesn't mean we should tax healthy choices, such as health club memberships or personal training sessions. That only goes toward making these healthy choices less affordable.
This obesity plateau means that our population is sitting at the top of a mountain from which they could tip forward to a healthier weight or backwards to further increase the obesity rate. The health club industry needs to do whatever it can to make sure it pushes forward.
We need to fight taxes on fitness memberships and look for ways to make memberships more affordable and desirable for the 84 percent of the population that does not belong to a club. And when people finally get to the point where they are ready to join a fitness facility, we need to make sure that we welcome them — truly welcome them — without judging them for their weight and offer programs that work for them, not just for the very fit. They need specifics on what to do, and they need a hand to hold.
In the end, people want to be healthy. No one wants to die of lung cancer or a heart attack at age 40 because they engaged in unhealthy behavior. Americans are hearing us, so let's step up our battle plan.

subset: commoncrawl


text:
White Sox enjoying early uprising with bats
KANSAS CITY -- The White Sox sat at No. 1 in the American League in OPS (.941) and slugging percentage (.549), and tied for the lead in runs per game (7.0) and home runs (six) through their first three games.
Last year's offense ranked last in the AL in runs scored (598), 14th in on-base percentage (.302) and 13th in slugging percentage (.378). Of course, playing 1/54th of a 162-game season does not a remotely significant sample size make. But there's no denying greater energy coming from the this year's group with the bats.
According to White Sox designated hitter Adam Dunn, hits equals energy, and that becomes the simple equation for success.
"It looks more exciting when you are getting hits. When you are not getting hits, it looks like you are flat and dead," Dunn said. "But the short story is, we are getting hits so everything is looking better.
"Hitting is contagious. If you can get everybody rolling early, that's what good offenses do. It's hard for an offense to struggle early and then everyone is looking up at numbers, and people saying this and that. A lot can block it out. A lot can't. That's how it snowballs and you get seasons you don't want."
Leadoff hitter Adam Eaton added that getting your pitch and doing damage with it, while swinging at strikes -- a focus of new hitting coach Todd Steverson -- represents half the battle.


text:
Introduction to Scanning Tunneling Microscopy
Additional materials available (3)
Licensed according to this deed.
Published on
This is a 60 second movie clip with an introduction to Scanning Tunneling Microscopy(STM).
Design goals are:
- Give an idea of what STM looks like
- Provide an overview of what STM does and how it works
Produced by Imran Sobh and Hyung-Seok Hahm
As part of instructional materials by National Center for Learning and Teaching in Nanoscale Science and Engineering
Sponsored by
National Science Foundation
Special thanks to Professor Joseph Lyding at UIUC for STM images
Cite this work
Researchers should cite this work as follows:


text:
Afilias provides a complete and flexible registry solution, combining Afilias' industry experience with best-of-breed technology to deliver a complete, integrated registry service.
83% of the world's population is estimated to be non-English speaking, yet for years they have been unable to communicate in their own languages via Web and e-mail addresses.
Afilias IDN E-mail is a software package that is standards-based and allows people to use almost any language in their e-mail address. The service allows full customization for the username of an e-mail address to be in any language/script represented in the Unicode standard, and can work with any second level IDN domain name or IDN top-level domain working on the Internet today.
Afilias' IDN E-mail package provides the following tools to e-mail providers interesting in integrating this technology into their e-mail service:
Key components of the package are based on open source software used in many production e-mail systems.
Our IDN E-Mail solution also works on a Mobile Phone.
Custom apps are available for a range of operating systems, and our Web environment is compatible with mobile Web viewing.It provides:


text:
Beyond the Census: New Opportunities at Commerce
May 12, 2020
Don't Let Cyber Insurance Distract You from Your IT Strategy
Mar 27, 2020
What the Consumption-Based Cloud Model Means to You
Mar 9, 2020
Arrow Electronics, immixGroup Offer IT Products in SLED Market via Cooperative Purchasing Program
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Four Keys to a More Painless FedRAMP Authorization
Dec 19, 2019
Activity Around DOD's New Cyber Certification to Heat Up in Early 2020
Nov 25, 2019
Census Action Far from Over Post-2020 Collection
Nov 22, 2019
DOD Putting IT Focus on 'Great Power' Competition
JEDI's Fundamental Message: Change is Coming
Nov 21, 2019
You Need FedRAMP, But How Do You afford It?
Nov 20, 2019
DHS CISO Puts Focus on Network Optimization, Monetization
Sep 11, 2019
What to Do When Cyber Hygiene Isn't Enough
Aug 9, 2019
JEDI: Patience you must have
Jul 3, 2019
To Win the Cyber Arms Race, Give Hackers Fewer Incentives
Jun 6, 2019
How to Talk Modernization to Unlock Sales Opportunities
Apr 16, 2019
Shutdown IT Planning: A Strategy for Continuity of Operations
Mar 7, 2019
Halt, Who Goes There?
Mar 4, 2019
Pentagon's 2019 IT Budget Appropriations target Cyber, AI and New Organizations
Feb 13, 2019
An Inside Look at DOD's AI strategy
Feb 6, 2019
30 Must-Read Federal IT Blogs 2018
Nov 30, 2018
Spending Insights on Display at Government IT Sales Summit
Nov 23, 2018
Where Opportunity Lies in the Massive Cerner-VA EHR Program
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DOD Reorgs to Fuel Cyber, AI and Space Opportunities
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It's not too late to grab some 2020 census business
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Oct 29, 2018
Inside the Burgeoning Market for IoT
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Stop playing "whack-a-mole" with your security
Aug 28, 2018
AI Wars: relax, it's not the end of the world
Aug 20, 2018
With new leadership, the JEDI saga enters new chapter
Jul 24, 2018
What you need to know as AI grows at DOD
Jun 29, 2018
How agencies learned to stop worrying and love the blockchain
Jun 21, 2018
Time to think of cybersecurity as a customer service issue
The GDPR initiative and how it affects US government and industry
Jun 5, 2018
How soon will AI be more than the next big thing?
Apr 24, 2018
The Top 3 cyber priorities for DOD's incoming CIO
Apr 23, 2018
Who will win as DOD cloud choices grow?
Apr 4, 2018
Regulation is the best hope for IoT security-imagine that!
Mar 12, 2018
Are shared services the answer to data protection?
Feb 13, 2018
What Trump's budget says about the administration's IT priorities
Feds to ramp up online purchasing presence
Jan 22, 2018
Why the cybersecurity industry is failing government
Jan 10, 2018
Federal IT analyst: Federal government shutdown would throw wrench in VA deal with Cerner
Dec 5, 2017
The top 3 hot spots for federal IoT security
Nov 29, 2017
Customer relationships drive success in uncertain civilian market
Nov 17, 2017
Sizing up the defense IT market for fiscal 2018
Nov 16, 2017
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Nov 13, 2017
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This is how government is getting hyper-converged
Oct 26, 2017
Too many acting CIOs put a damper on federal market
Oct 25, 2017
The need for better proactive cyber defense
Oct 12, 2017
State and Local Transportation IT Advances in the Face of Budget Austerity
Oct 7, 2017
Is State about to become the next big cloud service provider?
Sep 22, 2017
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Sep 5, 2017
Here are the top 5 public sector tech trends for FY18
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Is a Space Corps in our future?
Aug 21, 2017
Expect a more chaotic end to federal fiscal year 2017
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The biggest threat to cybersecurity is not enough info sharing
Jul 26, 2017
Government cloud adoption may make security easier
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Can technology help stop the opioid crisis?
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Internet of things requires agency-tech industry collaboration
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BMC sees growth accelerator in new partner agreement with immixGroup
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Most of GSA's $300M budget bump going to IT modernization
How 'smart cities' push IoT cybersecurity for state and local IT
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Army budget points toward multi-domain operations
May 15, 2017
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May 7, 2017
Technical concerns continue to muddle IoT's long-term promise
May 5, 2017
The intel community's love-hate relationship with the internet of things
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immixGroup's Tim Larkins talks security and innovation
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Inside VA's island of budget stability
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3 reasons to pay more attention to IAM in public sector
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Inside NASA's top 4 IT priorities
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Network security demands better procurement processes
Mar 2, 2017
HHS outlines lofty cloud plans despite IT budget squeeze
Feb 24, 2017
When disaster strikes, feds know what to do
Feb 22, 2017
O'Keefe: Federal experts agree IoT demands simple, baked-in security
Feb 14, 2017
5 government technologies poised for outsourcing in 2017
Jan 23, 2017
6 ways to transition to the Trump administration
Jan 3, 2017
3 Trends Driving DOD IT Budgets in FY 2017
Nov 24, 2016
Cloud Adoption Slowed by Culture, Even With FedRAMP
Nov 17, 2016
Automation and the Cloud to Drive IT Opportunities in 2017
The IT Ambitions of USDA
Oct 28, 2016
Year-End Deals on Track for Federal IT Providers
Oct 19, 2016
New federal data center policy to benefit IT
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FBI IT Budget Contracts, But Still Plenty of Opportunities
Oct 3, 2016
Reality Check: Philly's Cloud Ambitions Grind to a Halt After Transition
Sep 26, 2016
5 Hot Trends to Watch in Government IT
Sep 22, 2016
Ready for this Year's Government IT Sales Summit?
Sep 21, 2016
MobileGuard to Offer Messaging Security Tools Via immixGroup's Schedule 70, SEWP Contracts
Sep 14, 2016
Don't Miss These 2017 DOD Opportunities
Sep 9, 2016
Top Trends in SLED Technology
Sep 8, 2016
Public Sector's New View of Endpoint Security
Defense Department Drifts Toward Commercial Cloud
Aug 26, 2016
Three Ways Intelligence Will Be Using Big Data and Analytics
Aug 17, 2016
Bringing Clarity to the Clutter in Government Marketing (Episode 2)
Aug 16, 2016
8 Things You Must Do to Prepare for September
Aug 1, 2016
Three Ways Government is Using Drone Technology
Jul 21, 2016
Government Security News - June 2016 Edition
Jul 15, 2016
In Federal Cybersecurity, People Remain the Greatest Vulnerability
Jul 11, 2016
3 Ways the Air Force Future Operating Concept Will Affect IT
Jun 23, 2016
Questions Linger about GSA's Plans for New Cyber Offerings
Jun 20, 2016
Four Cyber Priorities for Government Leaders
Jun 17, 2016
4 Ways the Federal Workplace is Changing
Jun 16, 2016
Q&A with Steve Charles of immixGroup: The Changing Federal IT Landscape
May 31, 2016
New Data Center Policy Could Be Industry Boon
May 20, 2016
Government's Biggest Cyber Headache
May 19, 2016
Can You Clear Government's Cloud Hurdles?
May 18, 2016
Secure Cloud Computing in Government "Progress & Best Practices" 2016
May 17, 2016
immixGroup: DOD, DHS, Commerce Dept Land Top Spots in FY 2017 Govt Cloud Budget Rankings
May 16, 2016
Executive Interview: Steve Charles and Art Richer, immixGroup
May 6, 2016
DHS CISO Reveals Plan To Hire, Retain Cyber Professionals at immixGroup/FedInsider/GWU Hosted Event
May 4, 2016
DHS Sweetens Cyber Workforce Recruiting with New Bonuses
May 3, 2016
Channel Partnerships: CNSG, Arrow Bring on New Services
Apr 28, 2016
Public Sector Cloud Opportunities on the Rise
Apr 27, 2016
3 Things to Remember as Category Management Gains Ground
Apr 21, 2016
immixGroup Leaders to Receive Lifetime Achievement Award for Sales Excellence
Apr 20, 2016
Old Laws Are Keeping the US Navy from New IT Gear
Apr 13, 2016
Moving to the Cloud? Change Your Culture First
Federal Cloud Dollars Aren't Flowing to Platform-as-a-Service Providers
Navy IT Official: Laws Aren't Flexible Enough for Emerging Technology
Apr 12, 2016
Commands Increase Influence on IT Buying Decisions
Apr 11, 2016
Cybersecurity Spending Set to Soar in FY17
Mar 30, 2016
What's Driving Cyber Spending in the Federal Market?
Mar 29, 2016
The Art of Content Curation
Mar 25, 2016
2016 Brings New Tech Era For Washington
Mar 16, 2016
White House Proposes $3B IT Update Fund
Mar 10, 2016
Law Enforcement Modernization Offers Millions in Opportunities
Mar 4, 2016
Examining the State, Local IT Connection to National Infrastructure
Feb 17, 2016
Cyber, Cloud Lead DOD Budget Priorities
Feb 10, 2016
Finance, Health Care, Agriculture Play Key Roles in Critical Infrastructure Protection
Jan 20, 2016
Defense Department Assuming Growing Cyber Security Role
Dec 23, 2015
Feds Give Commercial Off-the-Shelf IT a Closer Look
Dec 17, 2015
Government Leaders See Partnership and Culture as Keys to Reducing Cyber Risks
Dec 15, 2015
Federal IT Opportunities: Steady Funding, Constant Challenges
Dec 9, 2015
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Dec 3, 2015
Defense Department Plays Key Role in Industrial Base Oversight
Dec 2, 2015
Centralization, Consolidation Reshapes Government IT Landscape
Nov 24, 2015
Integrators See Their Role Grow As Convergence Hits IT Management
Nov 20, 2015
Fiscal 2016 Filled with Defense Opportunities
Innovation Achieved Through Teamwork and Is Crucial to Future of Security
Nov 19, 2015
Military Healths Top 3 IT Priorities
Nov 11, 2015
Federal Investment in Big Data Applications Heads for Liftoff
Three Homeland Security Agencies to Watch
Nov 1, 2015
Opportunities Rising from Subtle Shift in Security Priorities
Oct 30, 2015
What the Gov't Cyberbreach Teaches Us
Oct 16, 2015
How to Target the Government's Big Data Sweet Spot
Federal Agencies Warming Up to Big Data
Oct 15, 2015
immixGroup Foundation's Eleventh Annual Charity Golf Tournament Raises $125,000 for Cancer Research
Oct 14, 2015
Three Keys to Better Channel Partnerships
Oct 6, 2015
U.S. Government Slow to React to Cyber Attacks
Sep 17, 2015
How The OPM Breach Galvanized Cybersecurity, And How You Can Help
Sep 3, 2015
States and Localities Partner Up on Procurement (Industry Perspective)
Aug 25, 2015
HHS Across the Country: A Regional Sales Overview
Aug 24, 2015
Top 4 Opportunities at FAA
Aug 18, 2015
A Channel Primer On Public Sector Procurement Co-Ops
Aug 17, 2015
In Public Sector Deal Registration, Lack of Transparency Challenges Trust
Aug 11, 2015
System Security: A Priority for All
Jul 8, 2015
DOD Combat Commands Need Situational Awareness
Jul 7, 2015
Big Data's $1.6B Role in DOD's IT Strategy
Want to Implement Emerging Tech Successfully? Start Small
Jun 19, 2015
Pentagon To Silicon Valley: Teach Us Big Data
Jun 18, 2015
Cybersecurity Needs Tech, Social and Policy Solutions
Jun 12, 2015
Here's How to Transform White House Policy into a Roadmap for IT Sales Opportunities
Jun 9, 2015
Navy IT Priorities Stress Security, Efficiency
May 18, 2015
The Government Doubles Down on Cybersecurity Leadership
May 13, 2015
Commerce Dept.'s Bottom-Line: Growing IT Opportunities
May 6, 2015
Software Vendors Be Warned: The Old Rules May No Longer Apply To Government
Apr 30, 2015
Five Cybersecurity Hotspots in a Reorganized DISA
Apr 23, 2015
Getting To 'Yes' In Government, Education IT Sales
Mar 31, 2015
3 Big Data Opportunities That Can Help Your Customers
Mar 30, 2015
Radio Interview with Tomas O'Keefe, Market Intelligence Senior Analyst
Mar 10, 2015
The Government is Doubling Down on Cybersecurity — Really This Time
Mar 9, 2015
Inside DHS' Big Data Opportunity
Mar 3, 2015
Inside the Private Sector's Role in the Health IT Opportunity
Feb 17, 2015
6 Ways FITARA Will Change Federal Sales
Jan 29, 2015
DHS Tech Spending — Where the Money and the Challenges Will be in 2015
Jan 26, 2015
The Key to Understanding the Gap Between the Vision and Reality of Cloud Computing
Jan 21, 2015
Here's How the White House Might Actually Improve Government Buying — For Real This Time
Jan 14, 2015
Flat Federal IT Market Means Vendors Must Work Harder Smarter
Dec 19, 2014
Happy Holidays: A Federal Cybersecurity Strategy Might Actually Be Forming
Dec 17, 2014
Federal IT Contract Forecast: Mostly Foggy
Dec 9, 2014
CDM Confusion Offers Sales Opportunities
Nov 14, 2014
Q&A: Guran Green, immixGroup VP of Corporate Development, Discusses How Contractors Can Tap into the State & Local Market
Oct 30, 2014
Add These Federal IT Influencers to Your Call List
Oct 27, 2014
Army Seeks to Simplify, Create More Interoperability for Information Systems
Oct 14, 2014
Not Up To Speed on Continuous Diagnostics and Mitigation? Then Wave Goodbye to Billions of Cybersecurity Dollars
Oct 9, 2014
7 Keys to DOD's Huge Health Opportunity
Sep 30, 2014
Summer of 2014 Highlights Need for Better Intrusion Prevention and Platform Approach
Sep 29, 2014
How to keep orders flowing until - and past - Sept. 30
Aug 27, 2014
At VA, all 2015 IT budget roads lead to VistA
Aug 25, 2014
How to stay afloat during cloud cutbacks
Aug 19, 2014
Federal Health IT Spending Trends, Today and Tomorrow
Aug 1, 2014
Where HHS 2015 IT budget is headed: Data
Jul 29, 2014
HealthCare.gov a debacle? It has also spurred some opportunity.
Jul 21, 2014
How to spot opportunities in VA's health record quest
Jul 16, 2014
Effect of the Federal Budget Cycle on Sales
Jun 13, 2014
Digging sales out of the mundane
Jun 11, 2014
Life As A Federal Small Business Or Subcontractor, And Growing Pains
May 20, 2014
Defense IT Spending on Downslope
May 12, 2014
Making the case for the $95B state and local market
May 6, 2014
A memo to the incoming federal CIOs
Cyber briefing on continuous diagnostics and mitigation (CDM) hosted by immixGroup
May 5, 2014
DOD says it needs a trusted supplier plan, but one already exists
Apr 15, 2014
How to target NASA $2B opportunity
Apr 7, 2014
GSA Schedules And The Trade Agreements Act – An Overview
How to find cybersecurity opportunities in the fiscal 2015 budget
Apr 2, 2014
Where to find growth in a tight DOD market
Mar 27, 2014
Op-Ed: Agency IT Budgets Aren't Keeping Pace with Malware Threat
Mar 20, 2014
Lost a Federal Contract Bid? How the Protest Process Works
Mar 11, 2014
Regulatory council gets in sync with SBA on small business program
Mar 3, 2014
Lessons from DOD's bumpy adoption of cloud computing
Feb 27, 2014
Some good news for small contractors in 2014
Feb 12, 2014
Military Services Snub Defense Information Systems Agency as Cloud Broker
Jan 16, 2014
BYOD In Defense Department? Not In This Lifetime
Jan 14, 2014
A Call for a Reasonable Path to Reasonable GSA Pricing
Jan 6, 2014
Federal Interactions: Employee and Hiring Requirements
Dec 20, 2013
Selling To The Government: Explaining The Procurement Integrity Act
Dec 13, 2013
Cloud may be slow, but it's going to be big
Dec 4, 2013
Federal cybersecurity policy (finally) begins to gel
Dec 2, 2013
Know The Rules: Restrictions Surround The Hiring Of Former Federal Employees
Nov 14, 2013
Do DoD changes signify last nail in iEHR's coffin?
Nov 11, 2013
Leaked intell docs offer insights on new opportunities
Oct 29, 2013
Unfair To Contractors
Oct 15, 2013
DHS Defends Federal and Critical Systems
Sep 27, 2013
Selling To The Government: Strict Rules To Follow On Gratuities, Gifts
Sep 24, 2013
Sequestration II: Expect Budget Rollercoaster In 2014
Sep 17, 2013
How Program Performance Evidence Can Provide Clues To Sales
Sep 10, 2013
Why The Year-End Spending Spree, And What To Do About It
Sep 9, 2013
SBA Achieves Benefits Trifecta With Latest Rules Changes
Aug 27, 2013
Government Contracts: Your Rights, Protective Clauses
Aug 12, 2013
6 Steps To Solving The Counterfeit IT Problem
Aug 5, 2013
Why Strategic Sourcing Hurts, And What To Do About It
Jul 10, 2013
Sharpen Your Sales Efforts to Match Today's Evolving IT Policies
Policies and Opportunities That Will Shape Cybersecurity Spending
Jul 8, 2013
Selling To The Government: Contract Types In Federal Procurement
Jul 3, 2013
Selling To The Government: What 'Fair And Reasonable Pricing' Means
Jun 28, 2013
Small Business Set-Asides Under GSA Multiple Award Schedule Contracts
Jun 27, 2013
Selling To The Government: Contracting Methods
Jun 21, 2013
Cybersecurity: The Octopus Embracing Government
Jun 3, 2013
The Sequester and IT Spending: Slow Growth, Not Cuts
May 31, 2013
Cyber Getting Baked Into More Procurements
May 24, 2013
The Cybersecurity Landscape in the FY2014 Federal Budget
May 23, 2013
Hoops And Hurdles: Standards, Requirements For Selling To The Government
May 21, 2013
Threats and Opportunities Growing in Cybersecurity
May 17, 2013
2014 Budget Request Points To New Opportunities
May 1, 2013
Ignore The Scary Sequester Predictions -- The Sky Isn't Falling
Apr 29, 2013
Selling To The Government: The Rules And Players To Know
Apr 15, 2013
Compromise Still Alive in Washington, At Least in Procurement Reform
Apr 2, 2013
Selling To The Government: The Art Of The Approach
Mar 7, 2013
DOD Gets Serious About Supply Chain Security
Feb 27, 2013
Defense Department to Wring Savings From Its Info-Tech Buys
Feb 18, 2013
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The other night we sat down to a dinner that was so simple it went from in my head to on the table in under 15 minutes. So I couldn't help but laugh when my husband commented, "this is my favorite dinner in a long time." – or something to that effect. He fumbled his words and tried to choose them as cautiously as he could knowing that his pregnant wife is in a constant fragile emotional state. I knew what he was trying to say and that it went beyond the fact that the number of warm, home-cooked meals I've produced in the last few months has dramatically decreased so he was just happy to see something other than grilled cheese.
It was the homemade baguette which accompanied the simple meal that really made his heart melt like the butter on his warm bread.
The next morning he happily sat down with his coffee and half a baguette, sliced and slathered with butter and jam. A breakfast that I too enjoyed nearly every morning living in a convent while studying art in Italy.
Bread is about as simple as it gets; flour, salt, water and yeast, yet I find there is nothing more satisfying and pleasurable than a slice of warm homemade bread.
I enjoy every aspect of it. Transforming sleeping ingredients into something that is alive and growing. The smell of the awakened yeast having a feast of flour wafting through the kitchen and flooding my head with memories of all the times I've smelled it before.
The anticipation of pulling the warm loaf from the oven and having to let it cool just enough so that you can cut through it without causing burns. And then finally the oohhh and ahhh inducing first bite that makes the hours and even days of wait all worth it and immediately sets your mind on making the next loaf.
Making baguettes at home can seem to be an intimidating task but I have been so happy with the results from this recipe. I think 12 loaves in about a week should be enough to prove how happy I've we've been with the final product.
Forming the loaves takes a bit of practice but I'm sure you will quickly get the hang of it after the second or third loaf. And even if they aren't picture perfect you will not be disappointed with the taste.
Thus I give my recipe for Homemade Baguettes or How to Turn a Simple Meal into a Memorable One.
Homemade French Baguettes
yields four baguettes
adapted from Artisan Breads Every Day, Peter Reinhart
5 1/2 cups unbleached bread flour (I've used all-purpose with great results)
1 tbsp coarse kosher salt
2 1/4 tsp instant yeast
2 cups warm water
Prep Day: Combine all ingredients in bowl of mixer, set with paddle attachment, and mix on lowest speed for 1 minute until well blended and smooth. Dough should form a coarse, shaggy ball. Let rest, uncovered for 5 minutes. Switch to dough hook and mix on medium-low speed for 2 minutes. Dough should be smooth, supple, and tacky but not sticky. Knead dough by hand on lightly floured work surface for 1 minute, then transfer to a large clean, lightly oiled bowl. Cover with plastic wrap and immediately refrigerate overnight or up to 4 days.
Dough can also be made without the use of a stand mixer. Combine all the ingredients – start with a wooden spoon then switch to your hands when the flour is incorporated. Lightly knead until all the ingredients are well blended. Let rest for 5 minutes then knead for about 5 more minutes until dough is smooth and slightly tacky.
Baking Day: Remove dough from refrigerator 2 hours prior to baking. Gently transfer to lightly floured work surface, taking care to release as little gas as possible. Divide dough into four equal portions. You can also do as I have by removing just enough dough to make one baguette and have a fresh baguette everyday for the next four days from one single batch of dough.
Form Baguettes: Pat each piece of divided dough into a thick rectangle.
Fold the bottom half to the center and seal the seam. Fold the top half to the center and once again seal the seam.
Roll the top half of the dough over the seam to create a new seam on the bottom of the loaf.
With seam side underneath, gently rock loaf back and forth, with hands moving out toward and increasing pressure at the ends, to slightly taper the loaf until baguette is the length of baguette pan (or baking sheet). Place the formed baguettes on a baguette pan or a baking sheet that has been oiled or covered with parchment. Mist top of dough with oil, loosely cover with plastic wrap, and proof at room temperature for about 1 1/2 hours, or until increased to 1 1/2 times its original size.
Baking: About 45 minutes before baking, preheat oven to 450 degrees. Place a sheet pan, which will serve as steam pan, with a 1-inch rim on shelf under which baguettes will be baked. Remove plastic wrap from the dough 15 minutes prior to baking. Just prior to baking, score the dough 1/2 inch deep with a serrated knife or razor. Transfer dough to the oven, pour 1 cup hot water into the steam pan.
Bake for 15 minutes, then rotate pan and bake for another 15-20 minutes, until the crust is rich golden brown, the loaves sound hollow when thumped, and the internal temperature is about 200 degrees in the center. Cool on wire rack before slicing or serving. Best eaten the same day, or heated briefly in the oven the next day if crust loses its crispness.
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Three historic lighthouses up for grabs, Penfield included
Scourge of neglect remains a threat
FAIRFIELD -- House for sale. Four bedrooms. Historic. Expansive views of Long Island Sound. Secluded location and solid granite construction.
You would expect to pay a few million bucks for a place like that, so you might be happy to learn that it'll likely sell for less than $50,000. But before you call your real estate agent for a drive-by you might want to know that the house, in this case, is more than a mile offshore, there's no electricity or plumbing, and it'll need about $500,000 in repairs to make it livable.
The house is the historic Penfield Reef Lighthouse, one of three in the state that the federal government wants to unload. If you still think you'd like to put in an offer, be forewarned: Many who have attempted to buy lighthouses only have bills from their attorneys left as souvenirs.
Treacherous sailing
The waters off Penfield Beach are regarded as the most dangerous of the Connecticut shoreline, which is why in 1870 Congress allocated $30,000 for the lighthouse. On Jan. 16, 1874, the light was lit and the foghorn sounded.
The first floor is granite block, and the second floor has wood-frame construction. Originally, there were four bedrooms on the second floor and a kitchen and living room were on the first level. Also on the first floor was the "oil room," where fuel oil was stored for the lamp. Heat for cooking and warmth came from a combination of coal and wood.
During much of its active life, Penfield had three or four men living there, one "keeper'" and two or three assistants. These men -- there were at least two husband-wife teams -- were civilian federal employees of the United States Lighthouse Service, which merged with the Coast Guard in 1939, according to Terry Pepper, executive director of the Great Lakes Lighthouse Keepers Association.
"Since the Coast Guard was a paramilitary organization, a lot of the keepers left the service in 1939," he said.
Turnover at the Penfield light was high, with most keepers discovering that manning a lighthouse was not quite as idyllic as imagined. Boredom was a constant adversary.
The Coast Guard manned the light until it was automated in 1971, at which point it considered tearing it down and replacing it with a light on a steel support, but locals objected. With help from then-Congressman Lowell Weicker and State Representative Stewart McKinney, the light was saved.
Saved from demolition, but not from slow degradation. In 2000, fears that the light would collapse into the lower level forced emergency repairs. While the lighthouse is secure to the elements, its interior is in shambles.
Hard sell
"If that light comes down, people in Fairfield can subtract 10 percent from the value of their homes," said Kaye Williams, who operates the nearby Captain's Cove Seaport in Bridgeport's Black Rock Harbor. But in spite of all of the love that people have for lighthouses, finding someone -- anyone -- willing to put up the money to restore and maintain historic lights is difficult, particularly for those lights that can only be reached by water.
The Penfield Light has been up for sale by the federal government's General Services Administration since 2007. It's about 1.3 miles offshore and there were even squabbles over the pile of rocks on which it sits. Some say it's in Bridgeport waters, others say Fairfield.
State oyster ground charts, which also determine criminal jurisdictional lines, have it firmly in Bridgeport.
Now these so-called "bottomlands" belong to the state. The federal government awarded control of the lighthouse to Beacon Preservation, which owns another historic light in Maine, several years ago, but that deal fell through when Beacon couldn't get ownership of the rock pile.
"I'd love to see somebody acquire it, renovate it to historical standards and maintain it as a great landmark for the region," said Fairfield First Selectman Michael Tetreau. "But the federal government wants way too much money for it, and the requirements on the purchaser are onerous. The amount of money that you'd have to put into it and the restrictions on renovating it make it difficult to make the financing work."
Too costly even for the town of Fairfield, whose elected officials have to justify to their taxpayers the logic of spending many thousands of dollars on a piece of property that most people would only see through a pair of binoculars.
"We've tried to get it, but even if they gave it to the town, with their restrictions, we'd have to spend $450,000 to fix it up they way they want," Tetreau said. "It's dangerous to get out there so even school groups wouldn't have access to it. And the next storm could take it down, so you'd be on the hook for all of the money you poured into it."
As for a potential owner, Tetreau said he's heard rumors, "but not anything credible."
Officials note that any potential owner would have to comply with the The National Historic Lighthouse Preservation Act of 2000, which states that in addition to maintaining the light, it "must make the station available for education, park, recreation, cultural or historic preservation purposes for the general public at reasonable times and under reasonable conditions."
So, if you want to buy a lighthouse and turn it into your own private hide-away, forget it.
Eyes on the Sound
To Chris German, CEO of the nonprofit boating advocacy group Connecticut Community Boating, federal and state governments are letting down the nation by unloading lighthouses.
"These are national historic treasures," German said. "Sure, there will always be a light there, but a light is not enough. You need eyes to go along with the light, and that's what's so important to protecting boaters -- and the rest of us, too."
German says lighthouses manned by human beings could go a long way in preventing the next terrorist attack.
"In this post-9/11 world, Long Island Sound could be thought of as the I-95 to New York City," German said.
German said the state, in partnership with the CCB, needs to create a Connecticut Light House Service, a modern-day rejoinder to the old U.S. Lighthouse Service.
"We will balance private interest with public benefit and save these structures for the public for years to come," he said. "We have the know-how, the vessels and the organization to do it, but we need the will and the support of the public to do it."
Lights for sale
Connecticut is home to 20 historic lighthouses, most built between 1800 and 1920. Many are only familiar to boaters because they're in out-of-the-way locations. The Stratford Shoal Lighthouse, for example, is so far out in the Sound there's debate on whether it belongs to New York or Connecticut.
Some, like New Haven's Five Mile Point Lighthouse, are owned by municipalities. Two are in private hands -- the Morgan Point Lighthouse in Groton's Noank section and the Stamford Harbor Ledge Light. Still others, although owned by the federal government, are kept up by private foundations, like the New London Ledge Light and the Falkner's Island Light off the coast of Groton.
Some are owned outright by historical groups; the Stonington Harbor Light is owned and operated by the Stonington Historical Society.
The GSA is seeking a nonprofit organization to take over the New Haven Ledge Light, one of the more impressive lights in the Northeast. One of the groups in the running is the New London Ledge Lighthouse Foundation, which is already striving to maintain the 104-year-old icon, according to Todd Gipstein, president of the foundation.
And like the Penfield Reef Light, the Saybrook Breakwater Light at the mouth of the Connecticut River -- and a stone's throw from Katharine Hepburn's former home -- is for sale.
German says the Saybrook Light, of the so-called "spark plug" design, holds a special place among anyone interested in Connecticut lore and history. It is, after all, depicted on the state's "Preserve the Sound" marker plate.
Still interested?
Even with deep pockets, buying a lighthouse from the GSA is a challenge.
There are two steps in the GSA's lighthouse unloading process. First, the GSA attempts offer the lighthouse to the state or a non-profit historic preservation group. This is what's happening with the New London Ledge Light. If there are no takers that meet GSA requirements, the property is auctioned to the highest bidder, as is the case with the Saybrook and Penfield lights.
Buyers typically have to comply with a raft of terms and conditions; the Penfield light, for example, comes with 25 pages of requirements that a new owner has to comply with, including allowing the Coast Guard "unrestricted access" to repair and maintain the light.
Rustic living
There's also a host of other challenges facing the owner if he or she wants to make the place livable.
"The place has some asbestos and lead issues," German said.
He also pointed out that living on a lighthouse out on the Sound isn't much different than living on an old boat. There's no indoor plumbing. Sea water is used for washing dishes and the like, and drinking water has to be brought from the mainland. Bathing would be a challenge. As for the toilet, a bucket would have to suffice, just like the keepers used in the old days.
"But you don't have that option now," German said. "You can't dump anything into the Sound."
Reporter Genevieve Reilly contributed to this report. email@example.com; 203-330-6403; http://twitter.com/johnburgeson


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BI 141/Fall 2013: Series 1 Lab 1 Boot Camp
This is the lab site for BI 141: Introductory Cell & Molecular Biology at Worcester State University. It is currently under development.
- 1 Recent updates to the course
- 2 Objectives: In this lab you will learn:
- 3 Series 1 Lab 1 Scientific Investigation Boot Camp
- 4 PART I: Metric Measurements
- 5 PART II: Basic Laboratory Equipment
- 6 B. Practice Exercises with Pipettes
- 7 PART III: Solutions
- 8 B. Solution Preparation
- 9 PART IV: Dilutions
- 10 PART V: Practice Quiz
- 11 Assignments
- 12 Links To Other Labs in this Series
Recent updates to the course
- This edit created a new page (also see list of new pages)
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2 June 2020
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Objectives: In this lab you will learn:
- Metric measurements and conversions
- Use of basic laboratory equipment
- Preparation of solutions
- Dilution from a stock
- Calculating effective concentration
Series 1 Lab 1 Scientific Investigation Boot Camp
In this laboratory, we will review the metric system as it applies to laboratory science, and learn how to use some basic laboratory measuring equipment by preparing a cupric chloride or cobalt chloride solution. You will also become familiar with the compound microscope by using it to examine the ciliated protozoan Tetrahymena pyriformis.
PART I: Metric Measurements
Measurements in pounds, miles and gallons are still commonly used in the United States, but the metric system, which was developed in France in the 1790's, has several advantages over the English system and is more convenient for scientific use. The metric system uses decimals and a system of prefixes to define the measurements of a variety of parameters. Since it is based on powers of 10, calculations using the metric system are simpler than the English system.
The metric units you will commonly use in the lab are:
To describe objects that are larger or smaller than the base units, a system of prefixes is used. The prefixes and corresponding exponential values you will commonly use to change the size of the base units are:
The power of 10 in the exponent column in the table above indicates the placement of the decimal point for that measurement. For example, 103 is equivalent to 1.0 x 103 and can be converted to 1000 by moving the decimal point 3 places to the right corresponding to the exponent value equal to 3. Similarly, 2.5 x 103 is converted to 2500 by once again moving the decimal point 3 places to the right. If the exponent is negative, the decimal point must be moved to the left the correct number of places. For example, we can convert 2.5 x 10-2 to 0.025 by moving the decimal point 2 places to the left. The use of exponents of 10 to place the decimal point is called scientific notation. Scientific notation is used to represent very large or very small numbers in calculations and scientific writing.
It is important to become familiar with measurements expressed in metric units and to be able to convert between these units (from grams to milligrams, for example). Conversions are straightforward since the power of ten between each unit is known. Since there are 1000 milligrams in each gram, a sample that weighs 1 gram also weighs 1000 milligrams. To keep track of the decimal point, 1000 milligrams is best written in scientific notation as 1.0 x 103 mg.
PART II: Basic Laboratory Equipment
In the first section of today's lab, you reviewed the metric system as it is used to describe lengths, weights, volumes and temperatures. This section contains descriptions of some of the laboratory equipment available to make these measurements. With these tools, it is possible to make solutions of known concentrations and to accurately measure portions of these solutions. In Part III of this lab, you will use some of the equipment to prepare a solution needed for Parts IV and V of the lab. You will be using several types of containers and measurement devices in the laboratory this semester. The following pages describe some of the basic tools needed in the first series of laboratories. As the semester progresses, you will be introduced to more complex equipment. Please review the following descriptions before you use the equipment today, and direct any questions to your instructor.
A. Descriptions of Laboratory Equipment
Beakers are used to prepare solutions and can range in size from 10mL to 4L. Any volume markings present on a beaker are only approximate and are not an accurate indication of volume. Beakers are not used to store solutions.
Erlenmeyer Flasks are used to prepare solutions and microbiological media. They range in size from 25mL to 6L. Volume markings on an Erlenmeyer flask are only approximate and are not an accurate measure of volume.
Tubes will be provided to you throughout the semester for experimentation. Round bottom glass test tubes will be used for making dilutions and biochemical assays. These tubes are identified by the diameter of the tube, so a 13mm test tube has a diameter of 13mm. A 16mm tube has a diameter of 16mm and can hold a greater volume of liquid than the 13mm. Centrifuge tubes can be glass or plastic, are often pointed at the bottom, and identified by the maximum volume they can contain. They are used in a centrifuge to separate biologic materials.
Graduated Cylinders are used to measure volumes from 10mL to 2L. The gradations on the cylinder are an accurate measure of the volume of liquid contained in the cylinder. The liquid in the cylinder will form a meniscus as shown in the diagram below. To measure the proper volume, the bottom of the meniscus must rest on the desired volume mark on the cylinder.
Serological Pipettes accurately measure volumes from about 1.0mL to 10mL using the gradations on the pipettes. They may be made of glass or plastic. Glass pipettes are washed and reused, while plastic pipettes are disposable. The markings on these pipettes are often in descending order, so to deliver 6mL using a 10mL pipette, the liquid must be drawn to the 4 mark on the pipette. To use the serological pipettes a green or blue pipette pump is attached to the top of the pipette. The blue one is used for smaller pipets (1ml or 2ml) and the green one is used for 5ml and 10 ml serologic pipettes. Never pipette by mouth. The pointed end of the pipette is then submerged in the liquid, and the liquid drawn into the pipette by turning the wheel on the pump until the correct volume is measured. To dispense the liquid from the pipette, simply depress the plunger of the pump. Some serological pipettes will dispense all liquid from the pipette tip; others are designed to retain a small amount of liquid in the tip. Remember the glass pipettes are not disposable. They should be placed tips down in the plastic pipette canisters at each bench after use.
Micropipettes are used to measure volumes less than 1.0mL. They may be fixed volume or adjustable volume pipettes. You are provided with 3 adjustable volume pipettes: one for 200-1000 microliters (μL), one for 20-200 μL, and one for 1-20 μL. To use a micropipette, attach a plastic tip to the end, depress the plunger on the micropipette to the first stop, and insert the tip into the liquid you wish to measure. Slowly release the plunger taking care not to trap any air bubbles in the tip or splatter any liquid onto the pipette base. Withdraw the tip from the liquid and dispense the volume into the desired receptacle by depressing the plunger as far as you can. Be sure to keep the plunger depressed until it is removed from the liquid, or you will remove some of the material you have just measured. Used tips are discarded in the trash or in the orange biohazard bag on your bench depending on what you have been pipetting.
Pasteur Pipettes are named after Louis Pasteur. They are glass dropper pipettes that require the addition of a separate bulb at the top to create the suction. Pasteur pipettes are not used to measure volume but are used to transfer liquid from one place to another. For example, Pasteur pipettes are often used to remove all the liquid from a tube after centrifugation.
Top Load Balances are used to weigh solid materials greater than 1.0 gram accurately. Either a plastic weigh boat or weighing paper is placed on the balance, and the balance is set to zero (tared). The solid material is then placed in the weigh boat or on the weighing paper for measurement. When the proper amount of solid material has been added, it is transferred to a beaker or Erlenmeyer flask, solvent added, and mixed until the solid material is dissolved.
Magnetic Mixers are used in the laboratory to facilitate the mixing of materials. They are particularly useful when making solutions. The mixer has a magnetic core that can rotate at various speeds. If a beaker filled with materials to be mixed is placed on the mixer and a magnetic stir bar added into the beaker, the mixer can be adjusted to mix the material at the proper rate. The mixing will continue until the mixer is turned off. A magnetic wand is then used to remove the stir bar from the solution before it is brought to final volume.
Vortex Mixers are used in the laboratory to mix materials contained in test tubes. The force of the mixing is adjustable, and the mixer can be set to run only when a tube is inserted into the mixer receptacle. The force of the mixer should be set so that the liquid in the tube forms a vortex as it mixes, and the tube containing the liquid should always be pointed away from you or your lab partner. If a tube is more than 2/3 full, a vortex mixer should not be used because the liquid will splatter out of the tube.
Centrifuges are instruments that use centrifugal force to separate biological materials. You will be using several types of centrifuges this semester. Clinical centrifuges are bench top models that accommodate various sizes of centrifuge tubes depending upon the type of holder installed. Refrigerated centrifuges are used when it is necessary to maintain the biological material at a constant cool temperature and can also accommodate several sizes of tubes. Microcentrifuges are used for very small plastic centrifuge tubes called microcentrifuge tubes. All centrifuges require that the centrifuge tube containing the biological material be placed across from a tube of the same weight. We call this "balancing the centrifuge." Tubes cannot be balanced by filling them to equal volumes, as these may not have the same weight. They must be balanced by weight, using a scale.
B. Practice Exercises with Pipettes
- Practice using the 10, 5, 2 or 1 milliliter serological pipettes by transferring the following volumes of deionized water into 13mm test tubes. Observe the volume differences. To insure the best accuracy, it is important that the pipette chosen to measure a specific volume has a total volume close to the volume to be measured. For example, the best pipette available to measure a volume of 1.7mL is a 2.0mL pipette. Remember that many serological pipettes have the volume markings in descending order, so be sure to draw the liquid to the appropriate mark on the pipette so the correct volume is dispensed.
- Adjustable micropipettes are very expensive, so please take great care in using them. To use a micropipette, attach a plastic tip to the end, depress the plunger on the micropipette to the first stop, and insert the tip into the liquid you wish to measure. Slowly release the plunger taking care not to trap any air bubbles in the tip or splatter any liquid onto the pipette base. Withdraw the tip from the liquid and dispense the volume into the desired receptacle by depressing the plunger as far as you can. Be sure to keep the plunger depressed until it is removed from the liquid, or you will remove some of the material you have just measured. Be sure never to hold a pipette upside down or sideways when liquid is in the tip. Also, if you feel resistance when changing the volume setting, stop immediately and ask your instructor for help. The following website Using a Micropipette has more detailed information about micropipette use.
Practice using the micropipettes by completing the following exercise. Keep in mind that this is an exercise designed to allow you to practice and test your pipetting technique. When carrying out experiments, it is always important to choose the best pipette for the volume desired.
- To test your micropipetting prowess and/or to calibrate your P1000, P200, and P20 micropipettes, first label 6 microfuge tubes (1-6) and weigh them on the top loading balance. Remember to zero the balance before weighing the tubes. Record the weights in the table below.
- Following the table below, pipet the specified volumes into the pre-weighed microfuge tubes prepared above using either your P20, P200 or P1000, as specified, then reweigh them. Record all weights.
- Calculate the weight of the water in each tube in grams. (1000 microliters of water should weigh 1 gram at room temperature.)
- If the water in any tube weighs more or less than 1 gram, your pipetting technique may need revision. Repeat steps 1-3 for the tube that is off the expected weight. If your water weight is significantly off after several repeated attempts, your pipette (or your technique) may need adjustment. Ask your instructor to watch your technique and/or to recalibrate your pipette.
PART III: Solutions
When doing biological experiments, it is essential that the exact concentration of every solution be known. It is also important to know the pH of a solution since pH is often critical in biological reactions. Buffered solutions, which can absorb minor challenges to pH, are often used to maintain the pH of a solution. Different ways of describing the concentration of a solution as well as pH and buffers are reviewed below.
A. Classifications of Solutions
1. Percentage by weight (w/v) Solutions The number of grams in 100mL of solution is indicated by the percentage. For example, a 1% solution has one gram of solid in a final volume of 100mL solution. To make this type of solution properly, you should weigh 1.0g of the solid material and dissolve it in slightly less than 100mL of solvent. Once the solids have dissolved, you can bring the volume up to 100mL. If one liter of a 1% solution is needed, then 10g of solid would be dissolved first in 975 mL of solution and then topped off to a final volume of 1000mL of solution to maintain the 1% ratio of solid weight to solution volume.
2. Percentage by volume (v/v) Solutions In this case the percentage indicates the volume of the full strength solution in 100mL of dilute solution. For example, a 60% ethanol solution is made by mixing 60mL of 100% ethanol with 40mL of water. If only 10mL of a 60% ethanol solution is needed, then 6mL of 100% ethanol should be mixed with 4mL of water.
3. Molar Solutions A 1 molar (1M) solution is a solution in which 1 mole of a compound is dissolved in a final volume of 1 liter (1L). For example, the molecular weight of sodium chloride (NaCl) is 58.44, so one gram molecular weight (1 mole) is 58.44g. If you dissolve 58.44g of NaCl in a final volume of 1 liter, you have made a 1M NaCl solution. To make a 0.1M NaCl solution, you could weigh 5.844g of NaCl and dissolve it in a final volume of 1L water.
Sample Problem: Convert a 0.2M solution of NaCl into %(w/v) units. 0.2M = 0.2mol/L = (58.44g/mol)(0.2mol)/L = 11.7g/1000mL = 1.17g/100mL = 1.17%(w/v)
4. Buffered Solutions A buffered solution resists changes in pH. The pH of a solution is a measure of its acidity, and is defined as the negative log of the hydrogen ion concentration. The pH scale ranges from 0 to 14 where 0 is the most acidic, 14 is the most basic, and 7 is neutral. Since pH is a log scale, the difference between pH 5 and pH 6, for example, is a factor of 10. Buffers are used when biological samples need to be kept within a narrow range of pH to maintain activity. The enzymes involved in biochemical reactions often require a narrow pH range. This range is usually 7.2–7.4 for human and animal tissues. A phosphate buffer is commonly used in the biology laboratory, because it exhibits excellent buffering capacity in the neutral pH range. It is made up of a mixture of sodium monobasic phosphate (NaH2PO4) and sodium dibasic phosphate (Na2HPO4) dissolved in water.
B. Solution Preparation
To prepare the solutions needed in later exercises, the 4 students at one lab table will prepare 2 different solutions. One pair of students will prepare 50mL of a 0.11M solution of copper (II) chloride dihydrate (MW=170.5) and the other pair of students will prepare 50mL of a 0.26M solution of cobalt chloride hexahydrate (MW=237.9).
- Calculate the correct weight of the material to yield 50mL of the proper molarity for the cupric or cobalt chloride solutions and record all calculations in your lab notebook. What would be the millimolar concentration of your solution? Have your lab instructor check your calculations before you weigh the material.
- Put on gloves before handling chemicals in the solid state. Nitrile gloves are available on the bench at the front of the laboratory. Please advise your lab instructor if you have a nitrile allergy.
- Using one of the top loading balances, weigh the correct amount of solid material and transfer it to a 150mL beaker.
- Using a graduated cylinder, add 35mL of deionized water to the beaker. Add a magnetic stir bar and mix on a magnetic mixer until all the solid material has dissolved. Remove the magnetic stir bar.
- Transfer the solution into an empty 50mL graduated cylinder and bring the volume to exactly 50mL with deionized water.
- Transfer the solution to an empty storage bottle. Label the bottle with the name and concentration of the solution, your initials, your lab section and date.
PART IV: Dilutions
A helpful animation of dilution making.
Many solutions will be provided to you this semester as stock solutions. A stock solution is a solution that has been prepared with a greater concentration of solute than is needed for a procedure, so it must be diluted to the proper concentration(s) before use. Consequently, it is very important that you master the concepts and techniques of preparing dilutions early in the semester. A dilution is made by taking an aliquot (a measured portion) of a stock solution, and adding to it an aliquot of a solvent (water or a buffer). This creates a new solution with a lower concentration of solute than was contained in the stock solution. Since the solute concentration of the stock solution is known as well as the volumes of stock and solvent, it is possible to calculate the concentration of solute in the dilute solution. The dilute solution is always less concentrated than the stock or starting solution. There are several ways to describe and prepare dilutions. Explanations are provided in this section as well as a laboratory exercise to introduce you to dilution preparation.
1. Ratio Method
This type of dilution describes the ratio of the solute volume to the total volume of the dilute solution. For example, to make a 1:10 dilution of a 1M NaCl solution, you would mix one part of the 1M solution with 9 parts of solvent (probably water), for a total of ten parts. If you needed 10mL of the 1:10 dilution, then you would mix 1mL of the 1M NaCl with 9mL of water yielding a total volume of 10mL. If you needed 100mL of the 1:10 dilution, then you would mix 10mL of the 1M NaCl with 90mL of water yielding a total volume of 100mL. The new concentration of NaCl in both solutions is determined by multiplying the stock concentration by the dilution ratio:
1M NaCl x 1/10 = 0.1M NaCl
2. Direct Calculation Method
Sometimes it is necessary to use an aliquot of one solution to make a specific volume of a more dilute solution. To calculate the volume of the more concentrated solution that will be needed to prepare the new solution, you can use the formula:
V1 x C1 = V2 x C2
V1= volume of more concentrated solution needed to make the new solution (usually the unknown)
C1= concentration of starting solution
V2= final volume of new solution
C2= final concentration of new solution (must be less than C1)
For example, if you have 2.5 ml of a 1M solution of NaCl and you want to make 3 ml of a 0.33M solution of NaCl, you can use the formula to calculate how much of the 1M solution you will need.
V1 x C1 = V2 x C2
(V1)(1M) = (3mL)(0.33M)
V1 = (3mL) (0.33M)/1M
V1 = 1mL
The calculations show that you will need to use 1mL of the 1M solution. Since the diluted solution is to have a final volume of 3mL, you can calculate the volume of solvent by subtracting V1 from V2 : 3mL – 1mL = 2mL. Therefore, adding 2mL of solvent to 1 ml of a 1M solution of NaCl will yield 3mL of a 0.33M NaCl solution.
3. Serial Dilutions
Serial dilutions involve the preparation of successive dilutions that vary the concentration of the solute by a constant factor, so that each successive dilution is a multiple of the previous dilution. For example, in ten-fold serial dilutions each successive dilution is one tenth of the previous dilution yielding dilution ratios of 1/10, 1/100, 1/1000, and so on. Serial dilutions may be used to prepare a series of standard concentrations for a biochemical assay, or to dilute a very dense culture of microorganisms to a concentration suitable for counting. It is very important to prepare serial dilutions carefully since each successive dilution will magnify the effect of any previous error.
How would you prepare a dilution series? As shown in Figure 1, you could begin with a stock solution of 1 mg/ml and set up a series of dilutions in which the dilution ratio relative to the stock solution progressively changes by one-half. In this instance, the concentrations of the successively diluted solutions would be 1/2, 1/4, 1/8, and 1/16 of the original concentration. If you needed 1 ml of each dilution, you could set up a series of 5 test tubes, with the first one empty and the rest with 1mL of solvent. You would then pipette 2mL (twice the volume needed for your test) into tube 1. Then transfer 1mL of that volume to tube 2, making a 1/2 dilution since there was 1mL of water in tube 2. After mixing the contents of tube 2, half that sample (1mL) would be transferred to tube 3, making a 1/4 dilution since the 1 ml of water that was already in tube 3 will dilute the incoming 1/2 dilution by 1/2 (1/2 x 1/2 = 1/4). This procedure is repeated until the last tube is reached, which will contain 2 ml of a 1/16 dilution of the stock solution.
B. Prepare Serial Dilutions of Cobalt Chloride and Cupric Chloride Stock Solutions
Each pair will use the stock solution you just prepared to create a series of 4 dilutions. Each dilution in the series will differ from the previous dilution by 1/2, so the dilution ratio for each tube is 1/2, 1/4, 1/8 and 1/16. Calculate the concentrations of cupric chloride or cobalt chloride that the tubes will contain after the dilutions have been prepared, and record them in your lab notebook.
- Label four microcentrifuge tubes with the dilution ratios: 1/2, 1/4, 1/8 and 1/16.
- With a 200 μL micropipette and clean tip add 100 μL deionized water to each tube. Discard the tip as instructed by your instructor.
- Using a clean pipette tip, add 100 μL of stock solution to the tube labeled 1/2, close the lid, and mix by inversion (not vortexing).
- Use a clean pipette tip to add 100 μL of the 1/2 dilution to the tube labeled 1/4; mix.
- Use a clean pipette tip to add 100 μL of 1/4 dilution to the tube labeled 1/8; mix.
- Use a clean pipette tip to add 100 μL of 1/8 dilution to the tube labeled 1/16; mix.
- Using a clean pipette tip, remove 100 μL of solution from the 1/16 tube, and discard it in the sink. You now have four tubes containing 100 μL of four different working concentrations of your reagent. Observe the color intensity of the four solutions and record in your lab notebook. Also, observe the relative volumes of the four solutions. If they are different from each other, then inaccurate pipetting has occurred. Save these dilutions for use next week.
PART V: Practice Quiz
Before leaving lab today, please take a practice quiz. This quiz will not count toward your grade in the course, but it will allow you to become familiar with the online Sakai quizzes that you must take before each lab. Your instructor will let you know how to take the quiz.
- Familiarize yourself with the Lab wiki and bookmark it on your computer. Read material relating to course assignments and lab attendance, as well as instructions about lab notebooks in the Introduction To Cell Biology page and in the Resources section. Be sure to familiarize yourself with the safety information (also found in the Resources section).
- Before you come to lab next time, read all the material in Lab 2 and outline the day's work in your lab notebook according to the example your instructor went over for Lab 1.
- After reading the material in the Lab 2 wiki, please take the Lab 2 online quiz through Sakai. Your instructor will give you more information about how to take this quiz.
- Make sure that you understand all the concepts covered in Lab 1.
- Solve the Lab 1 Practice Problems and submit them through Sakai. A copy of the problems are located below for your convenience. We recommend that you type your answers first in a word document and then paste the answers into Sakai. Assignments will ONLY be accepted through Sakai. Your instructor will show you how to do this in Lab 1. Your instructor will grade the problems and you will receive the results through Sakai.
Practice Problems (10 points)
Media:BISC 110 S13 Lab 1 Practice Problems revised.doc Download Assignment below in Word format through this link
Links To Other Labs in this Series
Lab 1: Boot Camp
Lab 2: Scientific Investigation in Tetrahymena
Lab 3: Scientific Investigation in Tetrahymena: Phagocytosis
Lab 4: Variable Testing in Tetrahymena: Phagocytosis
Lab 5: Scientific Writing & Effective Figure Design


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Constructed in 2009 in the highly populated South End neighborhood of Boston, Massachusetts, the National Emerging Infectious Diseases Laboratories (NEIDL) facility contains labs that operate at biosafety levels 2, 3 and 4. Due to its location the NEIDL has faced a raft of legal and regulatory hurdles that have prevented BSL-3 and BSL-4 labs from becoming functional. "Threading the NEIDL," is a 1-hour documentary narrated by Vincent Racaniello, PhD, Higgins Professor of Microbiology & Immunology at Columbia University, which explores how the NEDIL is secured from unauthorized entry, what's like to wear a BSL-4 level safety suit, how the facility is constructed to make it safe, and how workers carry out experiments with highly dangerous viruses such as Ebola virus and Lassa virus without jeopardizing their health or that of the surrounding community. This is a never before seen look at how one of America's state of the art biodefense research facilities operates and the security measures put in place to keep it safe, even in the heart of a major urban center. This documentary was filmed in conjunction with the popular science podcast This Week in Virology, which is also hosted by Vincent Racaniello.
Video is embedded from external source so download is not available.
Channels: Microbiology Interesting Science Medical
Tags: NEIDL Boston University Vincent Racaniello Alan Dove Rich Condit Elke Mühlberger American Society for Microbiolog
Uploaded by: microbeworld ( Send Message ) on 07-03-2013.
Duration: 56m 49s


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Regular price
These gorgeous cushions are a great way to display my art, supplied with inners, they are ready to add as a colourful accent, or to cuddle up with right away.
Choose either a super soft faux suede, with a grey or hot-pink back, or linen with a white back.
Sizes Available:45cm x 45cm (approx. 18" x 18")55cm x 55cm (approx. 22" x 22")


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Trump fires back on Twitter: 'Comey is a leaker!'
President Trump has some choice words for former FBI Director James Comey.
President Trump ended his Twitter silence early Friday, claiming ex-FBI Director James Comey vindicated him and accusing Comey of improperly leaking details of their discussions.
"Despite so many false statements and lies, total and complete vindication...and WOW, Comey is a leaker!" Trump tweeted.
Comey told a Senate committee Thursday that he believes Trump fired him over the Russia probe, and he accused the White House of lying about the details of the dismissal. He also admitted that he had leaked to the press memos describing his talks with Trump, saying he hoped the stories would spur the appointment of a special counsel to take over the investigation of the Trump campaign's contacts with Russia.
The former FBI director also appeared to confirm Trump's statements that, on three occasions, Comey told the president he was not personally under investigation with regard to Russia.
While Trump's attorney issued a statement defending Trump and attacking Comey for the leak, and Trump's son Don Jr. tweeted throughout the hearing, the president himself remained silent on the subject and did not tweet all day.
That changed early Friday.
One source of the Trump-Comey dispute: Comey's memos.
The former FBI director said he he kept notes on his meetings with the president because he was concerned Trump might lie about the nature of their conversations.
After his dismissal, Comey testified to the Senate Intelligence Committee, he told a friend about his memos and asked him to leak the information about them to reporters.
Trump and aides pounced on that revelation, accusing Comey of improperly leaking privileged conversations. They also disputed Comey's assertion that Trump asked the FBI director for a pledge of personal loyalty to the president.
The president and his aides backed other parts of Comey's testimony, however, including portions where the then-FBI director told the president he was not personally under investigation over Russia.
The FBI is investigating links between associates of Trump during last year's campaign and Russians who sought to influence the election by hacking Democrats. Comey said that Trump asked him specifically whether he could drop the investigation with respect to former National Security Adviser Michael Flynn, who Trump fired for withholding information about his contacts with foreign governments.
The president's critics said Comey's claims that Trump asked him about dropping the Russia investigation could amount to obstruction of justice.
"What struck me is that it is completely unacceptable for the president to ask the FBI director to be personally loyal to him and to ask the FBI director to drop it with regards to an ongoing investigation into his former national security adviser," said Sen. Chris Coons, D-Del.
Democratic Sen. Brian Schatz of Hawaii fired back at Trump on Twitter Friday morning, saying the president appeared to be accusing Comey of a serious crime: lying to Congress.
Read More:


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Browsing Department of Management, Politics and Philosophy (MPP/LPF) by Author "Fransson, Torsten"
Now showing items 1-1 of 1
Hansson, Finn; Brenneche, Nicolaj Tofte; Mønsted, Mette; Fransson, Torsten (København, 2008)[More information][Less information]
Abstract: In this report the key findings of an extensive literature review and an empirical survey of collaboration projects within the fields of sustainable energy and climate change are presented. The main objectives of the report is 1) to develop an analytical framework of innovation systems and to identify important managerial and organisational challenges pertinent to collaboration projects linking actors from within the Triangle of Knowledge (Innovation, Education and Research) and 2) to report on major collaboration patterns and on the basis hereof identify the most important types of collaborations known by the partners of SUCCESS. URI: http://hdl.handle.net/10398/6347
Files in this item: 1wpx1-2008.pdf (1.393Mb)
Now showing items 1-1 of 1


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Excellent graphics.
The previews are nice for the new album. Some good sounds.
I picked up the first Broken Harbour from Bandcamp and like it a lot. Looking forward to hearing the new album!
Just a quick bump to let people know that I'm down to just 2 copies of my self titled debut album:http://brokenharbour.bandcamp.com/album/broken-harbourI am not planning a repress, so once the physical albums are gone, they're gone.


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Krugman points out in his column today that Mitt Romney declared himself to be the confidence fairy in his Boca remarks, saying that he really didn't need to have an economic program, his election would be enough to lift all those boats without breaking a sweat. The declaration is as daft as it sounds, and Krugman dispatches it with his usual alacrity.
But within the column is this, which seems particularly relevant this morning:
It's true that some studies suggest a secondary role for uncertainty in depressing the economy — and conservatives have seized on these studies, claiming vindication. But if you actually look at the measures of uncertainty involved, they've been driven not by fear of Mr. Obama but by events like the euro crisis and the standoff over the debt ceiling. (O.K., I guess you could argue that electing Mr. Romney might encourage businesses by promising an end to Republican economic sabotage.)
You should also know that efforts to base policy on speculations about business psychology have a track record — and it's not a good one.
Back in 2010, as European nations began implementing savage austerity programs to placate bond markets, it was common for policy makers to deny that these programs would have a depressing effect. "The idea that austerity measures could trigger stagnation is incorrect," insisted Jean-Claude Trichet, then the president of the European Central Bank. Why? Because these measures would "increase the confidence of households, firms and investors."
At the time I ridiculed such claims as belief in the "confidence fairy." And sure enough, austerity programs actually led to Depression-level economic downturns across much of Europe.
None of that has changed the ongoing belief in austerity among our ruling class, however. They are, apparently, completely faith based at this point, unshakable in their belief in their Fairy Goddess. Here's Atrios:
If I actually believed that this was simply a story about evil plutocrats stealing from the rest of us I'd at least have a bit of respect. Oddly I don't actually believe that. Sure there are evil plutocrats stealing from the rest of us, ones who know what they're doing, but I also think that a lot of the people who rule us are just idiots.
Recession-hit Spaniards will this week be told to swallow yet more austerity as the government prepares a fresh round of reforms and another budget filled with spending cuts and tax increases that will allow it to seek a bailout from eurozone partners.
Pension freezes are also expected to form part of a raft measures to prepare the way for the European Central Bank (ECB) to give Spain support to control borrowing costs that will eat up a large chunk of next year's budget.
Apparently the Confidence Fairy has a huge appetite for human sacrifice.
Mort Zuckerman read from the Austerian Book of Common Prayer on The McLaughlin Group this past week-end:
According to Austerian dogma, the US is exactly like Europe we just haven't gotten there yet. So we must follow the European example and create as much suffering as possible as far as the eye can see to right the ship. (It's the same illogic as the one that says in order to fix a projected shortfall in Social Security a couple of decades from now we need to lock in that shortfall today.)
Zuckerman isn't even saying that the Confidence Fairy needs to be shown that we are serious about our debt so she will unleash her market power for the good of everyone. He's pretty much admitting that she's a vengeful Goddess who is punishing the lazy parasites for their piggish ways. She promises a dystopian hellscape in the future even for the deserving few unless we ensure that these parasites will continue to suffer now and in the future.


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Had been on a tour of Scandinavia and Western Europe from Monday(12-6-2017 to Sunday(9-7-2017) travelling like a local through public transport. Cycling is a way of life in Europe and in Barcelona came across this shop that gave cycles on hire.We in India should thank the fact that cycling is still a cheap hobby compared to other sports.A glimpse of the cycles on hire as also the rental charges.
Views: 91
Cycles and Common cyclists of European City's.
Location: City's of Europe.
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© 2020 People's Bank of Commerce. All Rights Reserved. Website designed by ProfitStars®.


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A place for atheists in the state of KY.
Members: 89
Latest Activity: Nov 2, 2016
Started by Mikayla Hensley. Last reply by Bluegrass Skeptic May 30, 2015. 2 Replies 0 Likes
Started by Cody Saylor. Last reply by Matthew White Sep 29, 2012. 1 Reply 0 Likes
Started by Larry Fiehn. Last reply by Larry Fiehn Apr 1, 2012. 3 Replies 0 Likes
Kiwi living in Catlettsburg, Boyd County, here. Homeschooling my son and feeling as if I am the only atheist for a good hour's drive! Hubby and son are atheist too, but we are the only ones as far as we know. We are also the only homeschooling family within a 45-minute, as far as I know - the KDE won't say either way. Hoping to find other like-minded people nearby.
Here's a list of Freethought Groups in Kentucky:
K.h. ky, if you're in Ohio County, Ky the Owensboro Humanists are right next door.
I'm right next to Cincinnati so really either side of the river will do.
Anyone in Northern Kentucky? Really looking for a group that meets to discuss.
Thanks Eric, I appreciate it, I don't drink however, but I will let you buy me a soda. Unfortunately I have not had the chance to get out much and explore, but Louisville is only 30 or so miles, so I need to put that on my list of things to do....
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Saturday, June 9, 2012
Pillow Talk swap round 8 {sneak peak}
10 comments:
I'm not in this swap Terri but I sure wish I was your partner after seeing this sneek peek! It's going to be gorgeous for sure!ReplyDelete
Oooh, lots of summery yumminess! Looking forward to seeing what this turns into :o)ReplyDelete
oh, so many lovely fabrics, looking forward to see the result.ReplyDelete
Olá,!Adorei o seu blog e já estou te seguindo,quero te convidar a conhecer o meu.ReplyDelete
te aguardo...
Wow, I'm so wondering what it will look like once it's done. Such beautiful colors and wonderful fabrics you are using.ReplyDelete
I still need to learn hexies and paper piecing. One day....
This looks great. I love English paper piecing.ReplyDelete
Oooh Fun! Love all those colors and fabrics :)ReplyDelete
Thank you for stopping by. I appreciate that you took a minute to read my post.
Please share what you thought about it with a comment.


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|Door to the orthodox synagogue in Presov, Slovakia
By Ruth Ellen Gruber
Jewish Heritage Europe -- the web site that I coordinate -- has just launched a monthly newsletter! The first edition came this week and contains a description of the web site's features as well as highlights from our regular almost-daily news feed.
JHE is an expanding web portal to news, information and resources concerning Jewish monuments and heritage sites all over Europe. A project of the Rothschild Foundation (Hanadiv) Europe, we foster communication and information exchange regarding projects, initiatives and other developments: restoration, funding, projects, best-practices, advisory services and more.
Our newsfeed is updated almost daily, and by now, with well over 500 posts, it represents a major searchable database of information on the contemporary status of Jewish built heritage in Europe.
Please take a look -- and subscribe and share!
Click here to see the Newsletter
Click here to see the Newsletter


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Spring awakening
Spring is here!
Women's satin bomber jacket with an all-over floral print, from Replay.
Playfully romantic
Hand-embroidered collar from the Italian label Vivetta.
Cult item
Roomy, streetstyle-inspired backpack by Givenchy. An ideal take-along on city trips.
Step into flowers
Chic update on the Stan Smith original, created in cooperation with The Farm Company.


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"We are powerless . . . We do not know what to do, but we are looking to you for help."
The Lord's response was:
Do not be afraid! Don't be discouraged . . . , for the battle is not yours, but God's.
It goes on to say:
21 After consulting the people, the king appointed singers to walk ahead of the army, singing to the Lord and praising him for his holy splendor. This is what they sang:
"Give thanks to the LORD; his faithful love endures forever!"22 At the very moment they began to sing and give praise, the Lord caused the armies of Ammon, Moab, and Mount Seir to start fighting among themselves. 24 So when the army of Judah arrived at the lookout point in the wilderness, all they saw were dead bodies lying on the ground as far as they could see. Not a single one of the enemy had escaped.
And then, the reason for the title of this post:
26 On the fourth day they gathered in the Valley of Blessing, which got its name that day because the people praised and thanked the Lord there. It is still called the Valley of Blessing today.
30 So Jehoshaphat's kingdom was at peace, for his God had given him rest on every side.
We are happily at peace as a family of 8, blessed by rest provided by God via our wonderful helpers! We are overflowing with gratitude. I've had trouble falling asleep at night, I am SO happy and thankful knowing all of us are under one roof finally. We are certainly praising the Lord for His faithful love and for the battle He has won - and not only the battle for Seth's earthly life, but also the ultimate battle over sin and eternal death when Jesus rose from the dead. We deeply appreciate those of you who are praying with us that each of our babies will accept the sacrifice He made to take away their sin, allowing them to come to God as His children. We're blessed beyond words by the fact that we can boldly approach the throne of grace as we have many, many times over the past year. Thank you "family members" for joining us there - we've certainly found abundant help in our time of need!
To God be the glory!
Carrie for us all
|Seth is smiling, although it looks a little goofy. Will's shirt says "Daddy's Big Guy" and
Seth's says "Mommy's Big Guy," and David is our very dapper smaller guy.
|United at last! Clockwise from bottom: Grace, Seth, Marcie, David, Will
|In birth order, counter-clockwise starting from 9 o'clock:
Will, David, Marcie, Seth, Gracie.
|Gracie looks like she's saying "Get off of me!" with a laugh.
David clearly thinks it's funny, too.


text: HumorPics Let's turn that smile upside down… written by Chad "LoganDX" G. February 5, 2015 Let's turn that smile upside down… was last modified: February 4th, 2015 by Chad "LoganDX" G. funnygeekGrumpy Cathumorpics 0 comment 0 Facebook Twitter Google + Pinterest Chad "LoganDX" G. A bit on the nuts side about video games and movies but boy do I suck at FPS'. Love my Xbox but still crush some Playstation. Run the Sarcasm Marathon, fluent in it. Would kill for a decent Zelda movie. previous post We need more instructions like this. next post Daredevil Trailer is Here Want more geek? Watch This: Take Your First Look At... July 25, 2018 GameStop Will Start Selling Comic Books In... June 12, 2018 NASA's Mysterious Mars Announcement Today June 7, 2018 These D&D Announcements Have Me Ready To... June 7, 2018 'Black Panther' Comics To Fill The Hole... May 27, 2018 Tabletop Fans Rejoice! Batman Tabletop Game Announced! May 12, 2018


text: The Mission Assignment Desk (任務受付, Ninmu Uketsuke) is located within the Ninja Academy where shinobi go to in order to receive most of their missions from the Hokage. The Mission Assignment Desk is located within a huge, wide-open space with six windows and high ceilings with two stick-like lights. The ceiling also has the kanji for the word "shinobi" (忍, shinobi) printed on it. There is a long table where the Hokage and other ninja sit when distributing the missions. On the front of the desk, there is a white banner with the kanji that says "mission assignment this way" (任務受付はこちらまで, ninmu uketsuke wa kochira made) and at the top is another banner that says "everybody, do your best" (皆さんガンバ, minna-san ganba). The Mission Assignment Desk was first seen when Team 7 completed their mission to capture the cat, Tora, for the Fire Daimyō's wife, Madam Shijimi.


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Here is the William Stafford Sport collection.
To help you find your ideal collection, we've split up our images into subjects. So if you're designing for a Sport theme, you can view all the appropriate images here.
Our London-based gallery is a treasure trove of fine-art reproduction prints, contemporary black and white photography, sepia photography and works in colour.
More Information
For more than 30 years William Stafford has provided classic and modern framed prints and photography to the design trade throughout the world.
If you have a question on our products we are here to help. Simply e-mail us and we will get straight back to you with an answer.
© 2012 William Stafford Ltd


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Travel and cooler rugs come in a variety of designs, colours and materials. Some of our popular cooler rugs feature breathable technology making them highly effective at wicking away moisture from the horse.
You'll find a great range available on our website, with many matching items such as saddle cloths and travel boots available. Some of our most popular products within the range come from Horseware, Masta and John Whitaker.
At Old Mill Saddlery, our buyers are horse owners, so each product that we sell has been carefully evaluated to ensure its suitability and durability. Rest assured, our products are of the highest quality.
Shop with us online and we can deliver your order worldwide.