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By Nathan Lerner and Len Lear
The first Philadelphia International Festival of the Arts, which will take place between April 7 and May 1, will be inspired by Paris during the years 1910 to 1920. A panoply of events, driven by food and fashion, will import the tastes and styles of the City of Lights to our own City of Brotherly Love.
Given its Francophile orientation, who would be the obvious choice for the festival's first annual Culinary Visionary Award? During his four decades in Philadelphia, Georges Perrier, 67, a Chestnut Hill resident who has achieved international acclaim as a chef and proprietor of Le Bec-Fin. The opening night gala at the Kimmel Center will honor the Lyon native for his contributions to the local culinary culture as well as his accomplishments as a regional member of the Maîtres Cusiniers de France (Master Chefs of France).
Perrier recounted his initiation into the local restaurant scene, "I was 23 when I first came to America (in 1967) and started my work in Philadelphia. Peter Von Starck was preparing to open Le Panetiere and hired me to be the opening chef. I just continued to stay because I fell in love with Philadelphia."
After three years, Perrier opened his own restaurant at 13th and Spruce Streets (where Vetri is now), choosing the name Le Bec-Fin, a French idiom for "the good taste." Last October, he celebrated 40 years of running what has become a Philadelphia institution, almost as familiar as the Liberty Bell or Independence Hall.
"I do consider myself to be an ambassador for French cuisine as I was the one who first brought this type of cooking to Philadelphia. I feel my continued work expresses that."
According to Perrier, he also performs a reciprocal role in promoting Philadelphia. "When I have the chance to visit France, I am always talking about the treasures we have here: the Kimmel Center, the Philadelphia Museum of Art and the Academy of Music. I brag about the great culinary talent, our ethnic restaurants and the bright cooking stars we have here as well."
Perrier reflected on how public tastes in food have evolved over the years: "I think people are more aware of what they are eating today and want to be educated about what is on the plate. My executive chef , Nicholas Elmi, uses lighter sauces with less butter and less cream than I used to do. I still make all the sauces, but I listen to him when he tells me, 'Not so much butter.'"
Perrier made extensive changes to the restaurant after the Mobil Travel Guide reduced it to four-star status in the 2001 Mobil Travel Guide. The interior was remodeled, the wine list expanded, and Perrier relinquished command of his restaurant to the head chef. Said Perrier to his staff, "Change everything but the chandeliers." In the 2003 guide, the fifth star was restored.
On April 4, 2008, Le Bec-Fin gave up its five-star rating in favor of a more relaxed atmosphere. This loss drops the number of American five-star restaurants to 16. The change to a more relaxed atmosphere includes à la carte dining as opposed to a price-fixed menu with strict seating schedule. Perrier even instituted a permanent four-courses-for-$40 option to make the restaurant affordable to middle-of-the-road diners.
In January, 2009, the French government awarded Perrier the "Legion d'Honneur." Signaling the end of an era, the one in which Le Bec-Fin helped start the 'Restaurant Renaissance' of Philadelphia, Perrier put the iconic building at 1523 Walnut St. up for sale last year, and he said he would close the restaurant in June of 2011, which generated wall-to-wall publicity. During New Year's Eve dinner at the restaurant, however, he made a U-turn, announcing that he would, in fact, keep the restaurant open.
Georges is also known for his explosive tirades and his passionate perfectionism in the kitchen. A November, 2009, article in Philadelphia magazine put it this way: "He strikes a match, sends his flambé sauce up in a mushroom cloud of flames. There is a beautiful violence to Georges Perrier's cooking, a mesmerizing, fiery tango of splashing wine and swapping pots and sauté pans on and off of the stovetop, like a race-car driver fighting for position. And of course there is the yelling and the screaming, Georges' rendition of Tosca. 'People just look at him and think he's bat-shit crazy,' says his chef de cuisine, Nicholas Elmi (now executive chef). 'And there's something to that. But he's one of the most generous people I have met in my life, ever.' "If only the world got to see soft Georges, cuddly Georges, a tad more often. 'To be good restaurateur,' Georges Perrier is telling me, 'to be an artist, we're all a little cwazy. So I am a little cwazy like everybody else. And sometime, I get carried away, I know I get carried away. This is not the way a gentleman like me should get carried away, but sometime it is difficult to control your emotion. I wish I do not have Latin blood, so I be nice and quiet like everybody else. But unfortunately, it is not the way I am.'" (The magazine referred to Perrier's still-thick accent as "Elmer Fudd slathered in a gooey béarnaise.")
Despite the accolades that he has accrued, Perrier, who recently put his massive Chestnut Hill home up for sale, is not resting on his laurels. He is expanding his off-site catering, and he is scheduled to open a new bakery soon, The Art of Bread, in Narberth.
PIFA will take place between April 7 and May 1. For more information about all of the events during those 24 days, call 215- 790-5800 or visit www.pifa.org.
Nathan Lerner, the Director of Davenport Communications, is actively involved in civic and cultural affairs. He welcomes feedback at firstname.lastname@example.com.


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StreamWriter - freeware internet-radio recorder
StreamWriter is a free application for windows that records music broadcasted by internet radio stations.
Current version: 188.8.131.52
Main Features:
* Record as many streams as you want at the same time (MP3/AAC)
* Automatically record a wishlist's song when it's playing on a stream
* Player for streams and recorded files
* Track splitting with silence detection
* Function for manual cutting of saved titles
* Tracks are named by a given pattern
* Short songs (ads) can be skipped
* Writing of tags to recorded files
* Script-based postprocessing
* Applying of effects to recorded songs (SoX)
* Integration of different audio encoders
* Scheduled recordings
* Stream browser
* Multilingual (English/German/French/Spanish/Polish/Finnish/Russian)
* Can be installed or used in portable mode
* ...and much more!
Website: https://streamwriter.org/en/

subset: commoncrawl


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The DSC-T100 doesn't simply stand out as expensive for such a slimline camera, it also stands out courtesy of its amazing 8-megapixel rating.
The 3-inch viewfinder is a great feature too. Slide back the lens cover and it instantly kicks to life.
The Carl Zeiss lens means you'll get pin-sharp clarity every time and as the menu is simple to use. With 31MB of memory it's got enough to get your started, but you'll quickly need to add a Memory Stick Duo Pro card to store more photos.


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Clean coal technology is constantly evolving. We're continually making use of this natural resource more efficient. I recently had the opportunity to tour the Prairie State Energy Campus for Designing Spaces: Think Green.
The episode is centered around the latest technologies and developments the coal-based electricity industry has been using to make this plant one of the most efficient and low emissions producing coal-fueled power plants in the nation.
Prairie State Energy Campus is the largest coal-fueled power plant today. It produces more than 7 million tons of coal a year, with the plant's first unit currently producing 800 megawatts of power.
Be sure to watch the segment here to learn more about Prairie State and clean coal technology.


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|Tier 1 Class
|Class traits
|Suggested Races
|Elf Wizard, Gnome Wizard, Orc Wizard
|Class Challenges
|No challenges
|Level 1 Mage Tower
|This article is in need of content, please consider adding to it.
The Wizard is the first class unlocked at the Mage Tower and a very flexible and potent spellcaster. The most obvious strength of the Wizard is the discount on casting costs, which is very noticeable with repeatable combat spells such as BURNDAYRAZ, APHEELSIK, PISORF, and BYSSEPS. A wizard can cast two fireballs out of the gate with no preparations, and quickly gains access to stronger combinations.
Wizards are able to find all the glyphs quickly, have the unique ability to hold all of them at the same time, and can accumulate significant conversion bonuses without the need to convert many glyphs. More than any other class, a Wizard is likely to have the right glyph as a tool to deal with specific situations or enemies.
Wizards usually want to start by making a beeline to all the glyphs before converting anything. Once all the glyphs are collected, they can buy cheap items from dungeon shops if nothing better is available, and convert them for bonuses. Holding 6 glyphs, each item converted will also collect 60 CP from the glyphs without destroying them.
Earthmother is a powerful deity for wizards, as her Greenblood bonus damage can be taken multiple times and is inflicted with every cast of BURNDAYRAZ or PISORF, and her repeatable Clearance boon gives a wizard exactly two fireballs every time. Holding on to IMAWAL until the late game to grab lots of bonus experience, together with utility spells to get around plants that spawn in bad locations without having to kill them, is also easy for Wizards to do.
Mystera may seem to be an obvious choice as the goddess of magic, although Mystic Balance is usually harmful to a Wizard who already has discounted spell costs. Still, she offers a couple of mana points for very little cost, and is generous with her piety. If no better deity is available in the endgame, Wizards can recover a significant amount of mana by converting their stored glyphs with Refreshment, and her Weakness boon can help with resists.
Although Binlor doesn't synergize that well with a dedicated fireball caster as his boons lean towards melee combat, he makes a good choice for Orcs, being the only deity to guarantee two spells including the mighty PISORF. His knockback boon also goes well with Orcs' enhanced based damage. His Stone Heart power is also one of the most efficient ways to wear down enemy resistances if there are enough walls on the level.
The wizard is flexible enough that most deities can be made to work well, with the notable exception of Taurog.
Orc wizards ({{OrWi}}, Orc Wizard) are one of the most versatile characters in the game, as they can easily stack up physical base damage while keeping hold most of their glyphs. As PISORF works off base damage, and the wizard gets a 25% discount on its cost, an Orc Wizard can make this spell a very powerful weapon. The Orc also gets a lot of use out of physical combat glyphs such as BYSSEPS, GETINDARE and CYDSTEPP.
Elf wizards ({{ElWi}}, Elf Wizard) are strong because more mana = more fireballs = good. The Wizard's Magic Affinity allows to exploit BURNDAYRAZ without requiring Mystera's Mystic Balance, and you can easily cast 4 or even 5 fireballs at once with an elf wizard.
Gnome wizards ({{GnWi}}, Gnome Wizard) are strong because mana is an important resource to wizards, so obviously more mana potions can mean a lot for the wizard, especially against bosses.
Elven Boots are available early on in the game and make an excellent prep item, allowing the Wizard to get to a 3-fireball mana pool easily. Especially if Extra Mana is also chosen. The Crystal Ball charges very quickly in the hands of a Wizard, especially combined with a deity that can help regenerate mana. Dragon Soul gives a lot of free spells over the course of the game, and is recommended when taken with a deity that lets you convert early mana into piety.


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Tyson Workers Caught Torturing Birds,
Urinating on Slaughter Line
From all-creatures.org
Action Alerts
Moo-ving people toward compassionate living
Your letters and calls do help!
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The calf photo on these pages is from Farm Sanctuary with our thanks.
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This is another of our Christmas week giveaway special. Read on for more detail.
As photos sharing become the norm is our everyday life, photos collaging has also become a favourite hobby of us. We have previously reviewed 5 photo collage software as well as PixelApp's Magic Collage and Photovisi. Today, we are going to take a look at Picture Collage Maker and see how it stakes up against the rest.
Just like any other photo collage software, Picture Collage Maker is a simple-to-use Windows program that turns ordinary photos and pictures into beautiful collage. It comes with several templates, so you can easily create collages, scrapbooks, posters, invitations, calendars, and greeting cards with just a few clicks.
The best thing about Picture Collage Maker is the number of templates that you can use. From the dropdown list, you can find templates for Calendar, Greeting Cards, Layout, Jumble or Simple. If you like none of the template, you can even start from scratch and design your own.
Drag and drop design
Picture Collage Maker is really easy to use. Once you have selected your template (or open a blank page), all you have to do is to drag and drop the respective photos/pictures to the template and apply the frames, masks, and various effects to the collage. You will be surprised how quickly you can get a stunning collage out in minutes.
Just like Photoshop, all the effects are layered. You can experiment with all the effects, and if you don't like it, just delete the respective layers.
Multiple options to export your collage
After you have finished the collage, you can either save it as a project file for future updating, or save it as a picture. Better still, you can set it as the wallpaper, send by email to a friend or get it printed on a t-shirt or mug.
Free Giveaway
Thanks to the developer of Picture Collage Maker, we have 20 license keys for the Picture Collage Maker Pro software to be given away.
Here is what you need to do to win the software:
Step 1
Be our Facebook fan or follow us in Twitter :
(If you have already done this, skip to step 2)
Fill up the form below. This is to make sure that we can contact you when you win the license. We promise that we won't spam you.
Update: This free giveaway contest is now closed.
Share this post either in Facebook or Twitter, or leave a comment below.
This giveaway begins now and ends Wednesday, 28th December at 2359hrs Eastern Time. The winners will be selected at random and announced via email.
Share and enjoy!
One comment
Looks like some great software! I have never won anything before, I hope this is my first.
Comments are closed.


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A new assessment of available water resources, published today by the Joint Research Centre (JRC), reveals that large areas in Spain and Eastern Europe have on average less than 200 mm freshwater available every year while the demand for water is three to ten times higher. The report 'Current Water Resources in Europe and Africa' shows variations in yearly freshwater generation from 10 mm to over 500 mm for Europe and from less than 0.1 mm to over 500 mm for Africa. The report outlines existing uncertainties and points to further research efforts needed for improved water management. Its publication coincides with the 6th World Water Forum taking place in Marseille, France, from 12 to 17 March 2012.
Ensuring that water is available in sufficient quantities for all legitimate uses is a major policy challenge. The JRC carries out research to assess current and future water availability versus current and future water demands from different economic sectors. It analyses available water resources, floods, droughts and water scarcity using hydrological simulation modelling. Improved water management and forecasts of water scarcity are however complex issues and this JRC analysis of the water resources for Africa and Europe provides a solid foundation on which to address future challenges.
In Europe, most data needed for water resources assessment are already available but access to them still needs to be improved to achieve a pan-European overview. In particular the public availability of river flow observations needs to be addressed.
For the African continent the latest assessment of water resources reveals large areas with less than 200 mm freshwater generation per year. Higher values are measured only in parts of Equatorial Africa, Southwest Ethiopia, Eastern South-Africa and Madagascar. Water scarcity is particularly difficult to estimate since the most recent pan-African data on water use date back to the year 2000. In addition, and just as for Europe, information on inter river basin water transfers is often unavailable.
The lack of available observed river flow data for Africa, for example, creates a major bottleneck in calibrating and verifying hydrological models for this continent. The availability of meteorological observations acquired by satellite has, however, greatly improved for Africa.
|Contact: Berta Duane
European Commission Joint Research Centre


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An interesting talk on WikiLeaks and our leadership.
Video Links : http://www.awaztoday.com/playvideo.asp?pageId=11984
Program Highlights :
Source : http://nadeemmalik.wordpress.com/2010/12/07/islamabad-tonight-wikileaks-our-leaders-2/
Guest: JUSTICE TARIQ MAHMOOD, SHAHEEN SEHBAI, MARIA SULTAN, KHAWAJA MUHAMMAD ASIF & DR A Q KHAN
TOPIC: Wikileaks, Role of Pakistani political and Military leaders
Shaheen Sehbai said that Pakistan top leadership is badly exposed. He said it is time for our leaders to clarify their positions. He said it is very important that our leaders clear this impression that they cannot take decisions without American advice and dictation. He said that like America, an official representative should be present in any such meeting with the Diplomats, and minutes of these meetings should be maintained.
Maria Sultan name is also mentioned in Wikileaks documents. She is one of those people who met with Americans. She said that she did not meet Ann Peterson instead she met with American Embassy officers after a conference on Fissile Material Cutoff Treaty (FMCT).
She questioned the authenticity of Wikileaks documents. She said we have blindly believed everything that Wikileaks documents revealed. Talking about American demand to Pakistan to return enriched uranium Maria said that American demand was not for Pakistan only but it was for every country.
She said that the conversations of our leaders should be recorded and submitted to some government institution or respective Ministries. Similarly, the need of officially maintaining record of all engagement, agreements, information sharing or military-to-military understanding should be officially maintained.
Dr A Q Khan commenting on one of the revelation of Wikileaks, that president Zardari promised to American ambassador to keep him confined to his house, said that he could tell by government tactics that they do not want him to walk free.
Dr Qadeer said that Pakistan should never sign FMCT unilaterally. He said Pakistan could do its utmost but America will never sign a civil nuclear deal with Pakistan.
Justice Tariq Mahmood said that American ambassador Ann Peterson offered to appoint Justice Iftikhar Chaudhry, as the judge of the International Court of Justice (ICJ) in a meeting with him. He said that the offer was to entice Justice Iftikhar to end lawyer's movement.
He said Americans always take care of their interest but we are not committed with the cause of Pakistan. He said that there is always one government official accompanying with the Americans when they meet some body to record their conversation. He said Pakistan also needs to practice same thing to keep a check on people holding top-most official positions in Pakistan.
He said we need to get rid of this thinking that without American support we cannot form government.
Khawaja Asif said that the only way we can free ourselves from American influence is economic progress in the country. He said without economic development, eradication of corruption and tight control on profligacy, Pakistan will continue to remain under the US influence.


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Strategic Initiatives
|The Donald W. Maine College of Business, through innovation, continuous improvement and quality initiatives, will enhance its reputation as a global leader in business education.
|Purpose Statement
The Donald W. Maine College of Business is a quality business school providing innovative career programs that appeal to a global audience. The College seeks to excel in applications-based instruction, enriched by faculty scholarship and professional business expertise along with service to the University's communities.
The strategic plan for the Maine College of Business revolves around the following goals and objectives. Each objective is then further broken down into strategies and specific action plans. The timelines, primary responsibility (ownership), outcomes/measurements, resources needed and use of results are all provided in tabular form below.
|To expose students to the necessary professional and academic abilities which embody the key practical and theoretical skills that will lead to success in their chosen profession (based on the Davenport University Excellence System).
|Objective A. To ensure that 100 percent of students are provided with the necessary theoretical academic foundation to ensure success in their chosen major.
|Objective B. To ensure that 100 percent of business majors are exposed to the necessary practical skills leading to professional success.
|To enhance academic programs by improving quality initiatives that will eventually lead to specialized accreditation for the College and various programs within the School.
|Objective A. Outline 100 percent of current academic offerings and conduct a gap analysis for all Maine College of Business programs per IACBE guidelines. (ACCOMPLISHED)
|Objective B. Evaluate other potential accreditations and identify at least one additional program accreditation to pursue, while ensuring the maintenance of currently accredited programs.
|To continue to grow and sustain the College's enrollment by actively recruiting qualified students (traditional /nontraditional).


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Where to Drink for Free in Honolulu
Summer is the perfect time to find a new favorite sparkling wine, bright and crisp and perfect for an Independence Day picnic, or to try a different kind of red in your sangria. Or maybe a sake-filled summer is more your speed. Here's a list of upcoming wine and sake tastings—and they're free. (We DO suggest buying a bottle or three when you find your new favorite bottle because there's no guarantee you'll be able to find them again.)
The Chinatown wine shop holds tastings every Tuesday and Friday from 5 to 7 p.m., while supplies last. Check the website for a calendar of tasting events.
Next up: HASR staff will pick a few of their favorite bottles and pour until there's no more on Friday, June 13. Show up right at 5 p.m. and you'll be sure to get more than a few good sips in.
535-9463, hasrwineco.com
The Wine Stop
The Wine Stop holds tastings every Saturday from 1 to 5 p.m. Check the website for a calendar of tasting events.
Next up: Take dad Saturday, June 14 to taste a few hand-selected, Father-approved bottles at The Wine Stop's Father's Day tasting.
On Saturday, June 21, The Wine Stop hosts a sampling of poke and poke-friendly wines. Because sometimes cool and raw trumps grilled and smoky. Wine Stop follows up its poke-fest with its Wines and Fireworks tasting—"sparkling wine for the sparklers, Syrah for the spice, Pinotage for the smoke and Zinfandel for the finish." We couldn't have said it better ourselves.
946-3707, thewinestophawaii.com
R. Field Wine Co.
Foodland's in-store wine shop, R. Field Wine Co., holds wine tastings every Saturday from 4 to 6 p.m. at Foodland's four R. Field locations. Check the website for a calendar of tasting events.
Next up: Head to any R. Field location Saturday, June 14 to sample a few bottles and to chat with the distributors who import the wines to the Islands.
Foodland Aina Haina: Ryan Choi of William Gladstone Imports pours a 2011 Famega Vinho Verde, 2011 Chateau de la Bouyere and 2010 Chateau Romfort.
Foodland Beretania: Mark Miyahara of Southern Wine & Spirits takes tasters to Tuscany with a 2012 Antinori Casasole Orvieto, a 2012 Antinori Cipresseto Rosato and a 2012 Antinori Santa Cristina Rosso.
Foodland Kailua: John Munro of Allied Imports goes old-world French with a 2012 Seigneurs de Bergerac Blanc , 2009 Chateau Haut Libarde and 2009 Chateau Coustaut La Grangeotte
Kapolei: Johnson Brothers distributors shows off a 2011 Tiki Sound Sauvignon Blanc, a 2010 Viberti Dolba Langhe and a 2012 Colimoro Montepulciano d'Abruzzo.
Fujioka's Wine Times
Fujioka's holds wine tastings Fridays from 4 to 7 p.m. and Saturdays from 3 to 6 p.m.
739-9463, timessupermarkets.com/fujiokas-wine-times
The Sake Shop
The Sake Shop on King Street hosts sake tasting at least once monthly. Check the website for a calendar of tasting events.
Next up: On Saturday, June 28, from 2 to 7:30 p.m., The Sake Shop will introduce sake enthusiasts (and newbies are welcome too) to nine sake from three Japan breweries in the Akita, Fukui and Nagano prefectures.


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Related Product Information
We offer several fluorescent and biotinylated phalloidin and phallacidin derivatives for labeling F-actin. These phallotoxins, isolated from the deadly Amanita phalloides mushroom,1 are bicyclic peptides that differ by two amino acid residues. They can be used interchangeably in most applications and bind competitively to the same sites in F-actin. Phalloidin and phallacidin contain an unusual thioether bridge between a cysteine and tryptophan residue that forms an inner ring structure. At elevated pH, this thioether is cleaved and the toxin loses its affinity for actin.
Fluorescent and biotinylated phallotoxins stain F-actin at nanomolar concentrations and are extremely water soluble, thus providing convenient probes for labeling, identifying, and quantitating F-actin in tissue sections, cell cultures, or cell-free experiments.1-3 Labeled phallotoxins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phallotoxin molecule per actin subunit in muscle and nonmuscle cells from many different species of plants and animals. Unlike antibodies, the binding affinity does not change appreciably with actin from different species or sources. Nonspecific staining is negligible, and the contrast between stained and unstained areas is extremely large. It has been reported that phallotoxins are unable to bind to monomeric G-actin.1 Phallotoxins shift the monomer/polymer equilibrium toward the polymer, lowering the critical concentration for polymerization up to 30-fold.3,4 Phallotoxins also stabilize F-actin, inhibiting depolymerization by cytochalasins, potassium iodide, and elevated temperatures.
Because the phallotoxin conjugates are small, with an approximate diameter of 12–15 Å and molecular weight of <2000 daltons, a variety of actin-binding proteins—including myosin, tropomyosin, troponin, and DNase I—can still bind to actin after treatment with phallotoxins. Even more significantly, phallotoxin-labeled actin filaments remain functional; labeled glycerinated muscle fibers still contract, and labeled actin filaments still move on solid-phase myosin substrates.6,7 Fluorescent phallotoxins can also be used to quantitate the amount of F-actin in cells.8,9 The unlabeled phallotoxins may be used as controls in blocking F-actin staining or in promoting actin polymerization. Our biotin-XX phalloidin allows researchers
to visualize actin filaments by electron microscopy using standard enzyme-mediated avidin/streptavidin techniques. Eosin phalloidin can potentially be used for correlated fluorescence and electron microscopic studies. Deerinck and co-workers reported that when eosin conjugates are excited in the presence of diaminobenzidine (DAB), an insoluble, electron-dense DAB oxidation product is formed that is easily visualized with either light or electron microscopy.10
Even though phallotoxins have an LD50 of approximately 2 mg/kg when injected into the mouse, the toxins of A. phalloides that are responsible for most of the symptoms and fatalities associated with poisoning by this mushroom are the structurally related amatoxins. The major in vivo lesions produced by injected phallotoxin occur in the liver and are associated with stabilization of polymeric actin. Phallotoxins, however, tend not to be absorbed by the gastrointestinal tract even though they are stable between pH 3 and pH 9.
Storage and Safety
Once reconstituted in methanol, the stock solutions are stable for at least one year when stored frozen at =–20°C, desiccated, and protected from light. It appears that NBD phallacidin, and possibly all of these toxins, exhibit a small loss of activity when stored in aqueous solution at 2–6°C for over three weeks.
While the amount of toxin present in a vial could be lethal only to a mosquito (LD50 of phalloidin = 2 mg/kg), it should be handled with care.
Materials Required but Not Provided
- Methanol
- Methanol-free formaldehyde
- Acetone or Triton X-100
- Fluorescent or enzyme-conjugated streptavidin, as prepared in step 1.9, for use with biotin-XX phalloidin only
- BSA, optional
- Image-iT FX Signal Enhancer (I36933), optional
- lysopalmitoylphosphatidylcholine, optional
Preparing the Stock Solution
Fluorescent Phallotoxins
The vial contents should be dissolved in 1.5 mL methanol to yield a final concentration of 200 units/mL, which is equivalent to approximately 6.6 µM.
One unit of phallotoxin is defined as the amount of material used to stain one microscope slide of fixed cells, according to the following protocol (see step 1.6), and is equivalent to 5 µL of methanolic stock solution for the fluorescent phallotoxins.
Biotin-XX Phalloidin
Staining cells with biotin-XX phalloidin (B7474) requires 1) the use of a higher concentration of the phallotoxin conjugate than when staining with fluorescent phallotoxins and 2) the addition of a fluorescent or enzyme-conjugated avidin or streptavidin detection reagent (see step 1.9). The vial contents should be dissolved in 0.5 mL methanol to yield a final concentration of 100 units/mL, which is equivalent to approximately 20 µM.
For biotin-XX phalloidin, 10 µL of the methanolic stock solution is equivalent to one unit of phallotoxin, which is defined as the amount of material used to stain one microscope slide of fixed cells according to the following protocol (see step 1.6).
Unlabeled Phalloidin
Solutions of this product should be prepared just like the fluorescent phallotoxins described in Fluorescent Phallotoxins, taking into account the larger quantity of material provided.
The procedure below was originally developed for use with NBD phallacidin.11 It has been successfully used with all of Invitrogen Molecular Probes phallotoxin conjugates. This procedure may not be optimum for a particular experimental system, but has yielded consistent results in most instances. The following protocol describes the staining procedure for adherent cells grown on glass coverslips.
1. Formaldehyde-Fixed Cells
- Wash cells twice with prewarmed phosphate-buffered saline, pH 7.4 (PBS).
- Fix the sample in 3.7% formaldehyde solution in PBS for 10 minutes at room temperature. Note: Methanol can disrupt actin during the fixation process. Therefore, it is best to avoid any methanol containing fixatives. The preferred fixative is methanol-free formaldehyde.
- Wash two or more times with PBS.
- Place each coverslip in a glass petri dish and extract it with a solution of acetone at =–20°C or 0.1% Triton X-100 in PBS for 3 to 5 minutes.
- Wash two or more times with PBS.
- When staining with any of the fluorescent phallotoxins, dilute 5 µL methanolic stock solution into 200 µL PBS for each coverslip to be stained. To reduce nonspecific background staining with these conjugates, add 1% bovine serum albumin (BSA) to the staining solution. It may also be useful to pre-incubate fixed cells with PBS containing 1% BSA or with the Image-iT FX signal enhancer (I36933) for 20–30 minutes prior to adding the phallotoxin staining solution. When staining with biotin-XX phalloidin (B7474), dilute 10 µL of the methanolic stock solution into 200 µL PBS for each coverslip to be stained. When staining more than one coverslip, adjust volumes accordingly. For a stronger signal, use 2 or 3 units per coverslip.
- Place the staining solution on the coverslip for 20 minutes at room temperature (generally, any temperature between 4°C and 37°C is suitable). To avoid evaporation, keep the coverslips inside a covered container during the incubation.
- Wash two or more times with PBS.
- When using biotin-XX phalloidin, incubate for 30 minutes with 100 µL of a 10 µg/mL solution of fluorescent or enzyme-conjugated streptavidin dissolved in 100 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.3% Triton X-100 and 1% BSA. Incubate for 15 minutes at room temperature. After incubation, wash the coverslip with PBS. To develop enzyme activity, follow a procedure recommended for the specific enzyme.
- For long-term storage, the cells should be air dried and then mounted in a permanent mountant such as ProLong Gold reagent or Cytoseal. Specimens prepared in this manner retain actin staining for at least six months when stored in the dark at 2–6°C.
2. Simultaneous Fixation, Permeabilization, and Fluorescent Phallotoxin Staining
The phallotoxins appear to be stable for short periods in 4% formaldehyde fixation buffers. This permits a rapid one-step fixation, permeabilization, and labeling procedure as follows.
- Prepare a 1 mL solution containing 50 to 100 µg/mL lysopalmitoylphosphatidylcholine and 3.7% formaldehyde and then add 5–10 units of fluorescent phallotoxin (approximately 25 to 50 µL of methanolic stock solution).
- Place this staining solution on cells and incubate for 20 minutes at 4°C.
- Rapidly wash three times with buffer.
- Mount coverslips and view.
Photostability or resistance to photobleaching is a primary concern when making fluorescence measurements. Invitrogen brand product lines such as Alexa Fluor, Oregon Green, BODIPY, and rhodamine fluorophores (including Texas Red™-X) are significantly more photostable than NBD and fluorescein and will therefore enable more accurate photographic measurements.
To further reduce photobleaching, minimize the exposure of fluorescently labeled specimens to light with neutral density filters and expose samples only when observing or recording a signal. Maximize collection of fluorescence by using a minimum of optics, high–numerical aperture objectives, relatively low magnification, high-quality optical filters, and high-speed film or high-efficiency detectors.
Invitrogen Antifade reagents, including Molecular Probes SlowFade Gold and ProLong Gold antifade reagents, can extend the useful lives of many fluorescent probes. These reagents are also available in a formulation with DAPI. They can be used on fixed cell preparations but are not compatible with living cells. Cytoseal also appears to protect BODIPY fluorophores from photobleaching.
- Wieland, T. in Phallotoxins, Springer-Verlag, New York (1986).
- J Muscle Res Cell Motil 9, 370 (1988).
- Methods Enzymol 85, 514 (1982).
- Eur J Biochem 165, 125 (1987).
- J Cell Biol 105, 1473 (1987).
- Nature 326, 805 (1987).
- Proc Natl Acad Sci USA 83, 6272 (1986).
- Blood 69, 945 (1987).
- Anal Biochem 200, 199 (1992).
- J Cell Biol 126, 901 (1994).
- Proc Natl Acad Sci USA 77, 980 (1980).
- Nature 284, 405 (1980).
- CRC Crit Rev Biochem 5, 185 (1978).
- J Cell Biol 106, 1229 (1988).
- J Cell Biol 103, 265a (1986).
- Eur J Cell Biol 24, 176 (1981).
- Proc Natl Acad Sci USA 74, 5613 (1977).


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Urban beekeepers are buzzing in Edmonton now that City Council has given the green light to a pilot project that will allow hives in backyards within city limits. On July 7, the Community Services Committee approved the pilot with urgency, pushing for the rules against urban beekeeping to come to an end. The City will now undertake a pilot and will report back with recommended bylaw changes in early 2015.
The battle to keep bees has been going on for quite some time and given that numerous other cities allow urban beekeeping, this decision was seen by many as long overdue. Importantly, it's another recommendation from Fresh that will see implementation, and it's a sign from Council that they are serious about food and agriculture in Edmonton.
Here's an audio story about the news:
Edmonton moves forward with urban beekeeping by Mack Male on Mixcloud
Further Reading
- Community Services Report: Urban Beekeeping Pilot Project
- City council approves backyard bee pilot – Edmonton Journal
- Urban beekeeping project could have Edmonton abuzz – Edmonton Journal
- Edmonton urban beekeepers buzzing into new hobby – Metro
- Bold beekeepers buck city bylaw – CBC
- Edmonton District Beekeeper's Association
- YEG Bees
- Pest Management Backyard Resources
- Audio clips courtesy of Dustin Bajer and the City of Edmonton.
- Thanks to mmorast for the beehive sounds!
- Check out more of Martin's photography here.
[view the original article via blog.mastermaq.ca]


text:
Wednesday, October 6, 2010
Impressions: Coal Fire
4 comments:
- pizzabloggerOctober 7, 2010 at 8:54 AM
Adam, nice write-up. I agree with your post 100%. Coal Fire Pizza has slipped in quality over the past year, something I lament heavily.ReplyDelete
And while the caesar salad at CFP is good,it's a sad day when that is the highlight of a meal. Cheers and best. --K
@pizzablogger: Your review was really helpful in putting this quick little impression together. As you'll discover in the upcoming Mia's review, I was hoping to get in touch with you to join us there, but it became a completely last-minute thing. Anyway, I wish the pizza we had eaten here had looked like the ones you had photographed last year!ReplyDelete
- AnonymousMarch 10, 2012 at 11:07 AM
Coal Fire? I see they put lots of thought into their name.ReplyDelete
@Anonymous: Yeah. They do use coal, but it's only one of three heat sources. "Coal Gas Infrared Fire" just doesn't have the same ring to it...ReplyDelete


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Adam Lambert to Release New Album in Early 2012
Adam Lambert says his second album will likely be out early next year.
The hotly-anticipated, as-yet-untitled, album follows Lambert's successful debut, 'For Your Entertainment.' A fan tweeted Lambert to ask about the album release timeline, and the 'American Idol' alum responded [capitalization Lambert's], "as of now, album will be released in the spring. Feb/march?"
The first single is expected to hit radio soon. Last week Lambert tweeted, "Seems as though the single will explode in November. I'm sorry for the delay- but trust me, it's worth it. The song HAS to be perfect!"
Lambert recently told Us Weekly that the album will feature "a more serious tone," adding, "It is an autobiographical album that portrays my dreams and aspirations. There are also some songs that deal with heartache and love. Whereas my old album was fun, campy, and over the top, this album is more contemporary and honest. The last album was more of a fantasy and this album is a search for my identity."


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Before I start belting out Sweet Home Alabama, I'll turn you over to a very funny Bama gal who is making a name for herself in romance fiction.
Take it away Kimberly!
I'm tickled to be here today. ~waves to my Alabama Home Girl, Christie~
One of the coolest things about being a writer is the fact I can file a lot of stuff under "Research" or "Refilling the Well." Going to Vegas for the weekend? Research. Reading the latest romance releases? Both Research and Refilling the Well. Watching movies? Surfing the net? A pedicure? I can apply the terms pretty broadly (it doesn't take a lot of practice), and it means I can get away with a lot.
"Gee, Honey, I can't wash the dishes right now. I know it looks like I'm just reading, but, really, it's market research. Gotta stay on top of the market, you know."
"The well is feeling a little empty at the moment. I'm thinking a Girls' Night Out is needed. You watch the kid, okay, Sweetie? Bye!"
I should be very ashamed of myself, I know.
So, I've spent a lot of time recently avoiding, I mean, researching. Or maybe I mean filling the well. All I know is that I've been watching a lot of Buffy the Vampire Slayer. And by a lot, I mean the complete box set. (Don't tell my editor, okay?)
I'm a huge Buffy fan. I'll happily argue Angel vs. Spike.* I'm unashamed to admit my small Riley crush. I've often worn my hair like Willow, and I would love to kick some butt like a Slayer. I love the action, the dialog, the characters. It was an awesome show.
Thing is, I write for Presents/Modern Heat. No vampires, demons, witches, or werewolves allowed in my plots. Nothing supernatural or paranormal. My heroines can be perky and strong, but they don't kick butt in the Buffy sense. Umm, maybe I can make a small argument on the whole Alpha-hero front, but even then, it's a little weak even with my broad definition of research.
But sometimes the brain just shuts down and it needs mindless entertainment. Or mine does, at least, because somewhere around Season Six, the fog lifted. I became oddly motivated and my WIP didn't seem like something I should continue to avoid. It seems Buffy slays all kinds of demons.
I coined a new term – I wasn't refilling the well; I was "Rebooting the Brain." I've decided it's equally as important. When my computer locks up and won't cooperate, everyone tells me to reboot. I think it works for the computer between my ears as well. Complete shutdown. Turn everything off and count to thirty before restarting.
Or in my case, watch six seasons of Buffy, then restart. Hey, I'm not one to question something when it works.
Am I the only one with this need to reboot? How do you reboot the brain? Alternatively, weigh in on the Spike/Angel argument. I'll pick a one person from the comment tail to receive a copy of my book!
*And I always come down on Spike's side.
I'm celebrating the release of TWO books this month! The Secret Mistress Arrangement makes its US debut (squee!) and The Millionaire's Misbehaving Mistress is out in the UK (squee again!) Big blog party at the Writing Playground (www.writingplayground.blogspot.com) on April 7th to celebrate – and more chances to win! Please drop by!
Saturday, April 04, 2009
Welcome to Kimberly Lang
Before I start belting out Sweet Home Alabama, I'll turn you over to a very funny Bama gal who is making a name for herself in romance fiction.
Posted by Christie Craig at 5:03 AM
23 comments:
- Christie Craig said...
Hi Kimberly,
I need to reboot. I love to go to Galveston, walk the ocean, to recharge. Thanks so much for guest blogging with us today.
- April 4, 2009 at 6:16 AM
- ♥ Dawn said...
I also recommend staying in touch with your local writing groups. I belong to two RWA groups and they are both great. Recently, one of our members gave a presentation on medieval armor, and we have a trip planned to Oheaka Castle. Bertrice Small gave a fascinating talk on the history of romance, and we had the Long Island Paranormal Investigators share their ghost hunting experiences. When I surround myself with inspiring people and interesting things, well, it's easy to be inspired myself. It's one of the reasons I like this blog. Oh, and Buffy rocks! Do you watch that Bones show? :) Keep up the great writing!
- April 4, 2009 at 7:37 AM
- Angel said...
Ooohhh, I love this idea! And I know exactly what you mean. All of a sudden, the desire to work on the wip suddenly comes to the fore, or dialogue and scene changes pop into your mind. It's a lovely feeling, isn't it!
- April 4, 2009 at 7:37 AM
- Kimberly Lang said...
Hi y'all!
Dawn-- I totally agree with finding a writing group. MY RWA chapter is a lifesaver on a regular basis. I don't have an "HOD Meeting." I have "HOD Day" where I meet friends for breakfast and Hubby knows he's on kid duty until whenever.
Angel, Christie -- I heart you both.
And now, in an amazing twist, I'm off to learn how to really kick butt Buffy-style in a self-defense class. This could be interesting...
- April 4, 2009 at 7:51 AM
- Christine said...
I know I need to reboot my brain when cleaning house suddenly seems very important to me. It's like my right brain is screaming for a way to get out and the only way to do it is with mindless activities.
Cleaning a few toilets apparently shuts down the left brain completely.
- April 4, 2009 at 8:25 AM
- amy kennedy said...
I love it that you've added re-boot to your writing lexicon. I think you are so right.
Just last year I did the same thing--watched the entire Buffy series, and at first I would say, "I can only watch it if I've written so many pages."
Yeah, that finally just morphed into me and the tv and Buffy, any free time I had. I think my husband now hates Buffy.
But once I finished it--I really could sit down and write freely.
So now...I'm watching the complete series of Angel.
And Kimberly, I go back and forth on the Angel/Spike thing--I'll take both please.
- April 4, 2009 at 8:26 AM
- Playground Monitor said...
Uh... I've never watched Buffy, but last year I discovered Law and Order SVU and when I get bogged down in romance, I just go watch detectives solve sex crimes. That's abour as far from category romance as you can get. I never knew I was rebooting my brain. I like that term -- and research too. It's a great excuse... er reason to have the TV on.
- April 4, 2009 at 8:51 AM
- housemouse88 said...
Hello Kimberly,
For me rebooting my brain means cleaning the house or changing my routine.
Now the Spike/Angel issue, I have to chose Angel. There's just something about him that I can't shake.
Have a great day.
- April 4, 2009 at 9:01 AM
- Allison Knight said...
Love the expression "reboot". Since my DH is a bit of a geek, I believe that's going to be my new password when the dishes don't get done, bed time comes and I'm still reading, I forget to defrost something for dinner.
Thanks. Just the word I need.
Great Blog!
- April 4, 2009 at 9:05 AM
- Katherine Bone said...
Waving at you, Kimberly!! I've been looking for THE SECRET MISTRESS ARRANGEMENT. Haven't found a copy yet, but I did order it from Barnes & Noble.
I always liked Angel's angst. Stopped watching Buffy before Spike came along.
Rebooting is a great term. I need a lot of rebooting right now. My brain is fried. ;)
- April 4, 2009 at 10:29 AM
- Lynn Raye Harris said...
Ooh, I like rebooting. And I guess I have to admit I've never seen Buffy. But I am a huge Joss Whedon fan when it comes to Firefly. One of these days, I'll get around to Buffy.
I like to reboot with a variety of things. Sometimes it's shopping, sometimes television, sometimes reading. Right now, I feel like a great new pair of high heels could do it. ;-)
- April 4, 2009 at 10:35 AM
- LuAnn said...
We don't have a writing group in my area, but I do read posts by some on the Internet.
- April 4, 2009 at 10:54 AM
- Misty Williams said...
I need to reboot. NOW! LOL.
I like to watch NCIS! Bones is a good one as well. I will watch a movie or read a book. I would really love a great place to walk around here, because that always seems to help me. However, there's not a good place to do that here. Now where I can enjoy what I see around me.
I would go with Spike as well in Buffy, but have to admit I haven't seen as much of Buffy as you Kim. :)
- April 4, 2009 at 11:10 AM
- Suzan Harden said...
Um, as a die-hard, 'watched the original theatrical movie', 'bought the Buffy and Angel seson sets the week they first became available' fan, I have to admit - neither. I've got a thing for Giles - but then I did ever since Tony Head made those Taster Choice commercials back in the '80's. ;-D
- April 4, 2009 at 12:58 PM
- Refhater said...
I reboot by taking pictures. I take my camera and wander aimlessly taking hundreds of pictures. Then I spend time deleting the bad ones and editing the good ones.
I never was a Buffy/Angel fan, but I do like to watch David Boreanaz on Bones.
- April 4, 2009 at 1:24 PM
- Estella said...
When I need to reboot I grab a book, sit down and read.
I have to confess, I have never seen an episode of Buffy.
- April 4, 2009 at 2:22 PM
- Kimberly Lang said...
Seems we have a lot of folks who have discovered the importance of rebooting!
I'm shocked to see so many folks who haven't watched Buffy! I must admit I was forced to watch it the first time by my Geek, but I was hooked pretty quickly. I haven't watched Bones, but I may have to give it a try.
- April 4, 2009 at 2:45 PM
- Christy Reece said...
Hey Kimberly, congratulations on your releases. That's awesome!
As a self-confessed Buffy fanatic, I will agree that watching it for hours and hours will give your mind all sorts of 'rebooting' fuel. Of course, just about anything that Joss Whedon is involved in is brilliant. Anybody watching Dollhouse?
Um...Spike or Angel? I'd have to go with Angel. But I admit, I kind of had a crush on Giles too.
- April 4, 2009 at 3:39 PM
- Callie James said...
Hi, Kimberly. Many congrats on the releases!
Southern Magic member and Buffy fan waving!!!
I've done many of the things listed here to fill the well, but I did a massive hard boot when I switched genres. I used to write romantic suspense. Now it's romantic suspense paranormal, and I can't shut off the creativity!
A cross I am willing to bear...
Awesome blog!
- April 4, 2009 at 5:46 PM
Congrats on the new releases! Great blog.
- April 4, 2009 at 6:23 PM
- Mary Marvella said...
Excellent blog. You made me smile and I know you're thrilled about the books!
- April 5, 2009 at 2:37 AM
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- January 16, 2010 at 6:35 PM


text:
In this case, I almost upended the order of my presentation so I could make use of a tactic that has worked well before. It even fit with my topic. Fortunately, I stopped myself, thinking, "Remember Coco."
That's Coco Chanel, the epitome of elegance, who's said to have advised women "Before you head out the door, take one thing off" from among the accessories you've put on. It's advice that speakers would be wise to consider, lest their presentations start looking like a Christmas tree, decked out from top to bottom. There's a temptation to think that just one more thing will "make" the presentation, when in fact it might detract from your impact.
When gauging what's too much in your presentation, you might need to remove:
- Slide jewelry, like animations, transitions, bullets, videos and sound, or too many charts, pictures and graphics. Pile on those cone charts and shadings only if you want us to start counting how many times you've done that.
- Audience stylings, like putting questions upfront, taking polls of the audience or using volunteers to demonstrate key points. At some point, you may look as if you're distracting us from a lack of content.
- Technology tinsel, from laser pointers to slick videos. You may dazzle us, but will we remember your point?
- Language lightshows, such as using an alliteration with an analogy with a story. Too many rhetorical devices make us think about your machinery, not your point. Be confident in your content, and don't deck it out with boughs of holly.
|This post was featured on Ragan.com


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How High Can the Katrina Price Tag Go?
It is Katrina anniversary week, and news outlets are abuzz with stories probing why, 12 months after the worst natural disaster in American history, so little progress semms to be made. USA Today notes that the price tag for recovery and reconstruction stands at $122 billion, and shows no signs of slowing its ascent.
That's still much less than half of what the war in Iraq has cost the just the United States so far; the amount spent on that conflict recently surpassed $300 billion. And more than twice as many Americans have died in Iraq.
It is important to note that the war in Iraq is very much man-made, and was very much voluntarily created.
We at CorpWatch strive to put numbers like these into perspective. Our new report, "Big, Easy Money: Disaster Profiteering on the American Gulf Coast" explores where all of that money appropriated to Katrina relief so far has gone. And the answer is: into the same pockets as much of the money appropriated for the war. Huge multinational corporations such as Halliburton, Bechtel, AshBritt and CH2M Hill (who have well-documented ties to the Bush Administration and/or members of Congress) have made a fortune from no-bid and contingency contracts to rebuild Afghanistan and Iraq, and have also received similar contracts to clear debris and rebuild the Gulf Coast. And the very same problems have emerged: overcharging, underperformance, and a near complete lack of accountability.
- 21 Reconstruction


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Hi Everyone, WillCFish here,
I wanted to post info on a children's book that I think will interest those of you who have fishing kids in your lives. GONE FISHING by Tamera Will Wissinger is a new fishing adventure story for kids age six and up told through a series of poems. It's about a nine-year-old boy who is excited about his fishing trip with his dad and it includes the challenges and thrill of fishing for the big one.
Tamera is my daughter and some of the poems in her book were inspired by fishing trips that my wife and I took with our kids when they were young. I've had the chance to read an advanced copy and it will spark the interest in fishing for children as well as give them a better understanding of Poetry.
Publishers Weekly and the Junior Library Guild have already given GONE FISHING their endorsement, too. GONE FISHING is available for pre-order now, and will release on March 5. Here's a link to Houghton Mifflin's ordering info:
http://www.houghtonmifflinbooks.com/hmh ... 0547820118


text:
NOT HAPPY: Adult Migrant English Program (AMEP) students at TAFE Illawarra's Wollongong campus expressed their anger with the decision during a rally on May 4.It's old news TAFE Illawarra will no longer run theAdult Migrant English Program (AMEP).
But revelationsthe highly-popular program will now be run by MAX Solutions has come as a shock to students.
Last month the Federal Government originally awarded the contract to Navitas, who has since sub-contracted AMEP to fellow training provider Max Solutions.
A Max Employment spokesman confirmed to the Mercury that its Wollongong office will deliver the program from July 1, 2017.
That's well and good for Wollongong-based students, but uncertainty remains for students in Eden Monaro, Gilmore and Newcastle.
Hunter TAFE also lost the AMEP contract to Navitas. NSW Teachers Federation said this decisionwill put 26 teaching staff under threat (including 2 full time teachers) and affect 200 students.
NSWTF organiser Robert Long said more than 70 teachers and support staffing positions are under threat and over 200 students will be impacted in the Illawarra/South Coast region.
He said small campuses like Moruya and Ulladulla will be heavily impacted in the Gilmore electorate.
''Teachers from our smaller rural colleges are worried they will not have adequate funding to keep their foundation studies sections open,'' Mr Long said.
''Teachers have expressed grave concerns about the impact of further cuts and the ability to provide language, literacy and numeracy support to their students, including apprentices.''
Eden Monaro MPMike Kelly said it wasnot fair that the Liberal government ''is again chipping away at the education services of rural and regional towns''.
''Moving to a new provider for adult migrant literacy will see the loss of the existing highly trained and experienced TAFE teachers who make a real difference in student outcomes,'' he said.
Newcastle MPSharon Claydon was concerned that Max Employment may not have the expertise or onsite facilities to provide the standard of training and service that TAFE has been delivering in Newcastle for over three decades.
Cunningham MP Sharon Bird this was just another attempt by the Liberals to decimate TAFE in regions like the Illawarra.
''The new provider – whether it is Navitas or MAX Solutions – doesn't have teachers, doesn't have classrooms, whereas I understand TAFE Illawarra had a AAA rating in the previous Commonwealth contract,'' she said.
''The many students I have met with are very distressed by this decision and the significant impact it will have on them as they have rented properties close to the TAFE, have their kids in local schools and use the childcare facilities at TAFE.''


text:
A California woman found a chunk that was from a meteor spotted last week. NBCNews.com's Katy Tur reports.
A chunk of meteorite struck the house of a San Francisco Bay Area resident, landing in her backyard, after a meteor streaked through the sky on Wednesday evening.
Lisa Webber found the 2-inch rock, weighing 63 grams, in her backyard on Saturday after reading an article in the local paper about the meteorite.
She remembered hearing a strange noise on Wednesday, but thought that it was an animal, SFGate.com reported. After finding the chunk on Saturday, along with a dent on her roof, she and a neighbor's son put a magnet to the rock and the two stuck together.
"It's just science -- and it's cool," Webber, of Novato, Calif. told SFGate.com. "It's wonderful. It's like the heavens coming down, and history and this thing probably came from an asteroid belt between Mars and Jupiter -- I mean, how cool is that?"
Investigators at the non-profit SETI Institute inspected Webber's find and declared it authentic.
NBCBayArea.com: Wednesday's meteor falls on North Bay home
"The significance of this find is that we can now hope to use our fireball trajectory to trace this type of meteorite back to its origins in the asteroid belt," said Dr. Peter Jenniskens, a SETI Institute investigator.
Jenniskens and his crew believe that larger pieces of the meteor are out there and hope to find others.
NBC News staff contributed to this report.
More content from NBCNews.com:
- Source: No deal yet on US-Iran nuclear talks
- Father dies shielding children from gunman who set home ablaze; boy killed
- Mystery ground shaking rattles South Jersey
- US nurse arrested in Macedonia awaits verdict in coin-smuggling trial
- Video: Texas school district tracks students with ID cards


text:
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More Swiss Authors
Johanna Spyri
· Heidi
Max Frisch
Johanna Spyri: Heidi, the Girl from the Alps
Heidi, the Girl from the Alps, a two-volume novel written by Johanna Spyri (born in 1827 in a small village, died in the city of Zurich in 1901), was published in 1879 / 1880, became a world-wide success story already towards the end of the 19th century and children do still like it today - as a book, as a radio play and as a movie.
Heidi is by far the most popular piece of Swiss literature ever written and has been translated from German into 50 languages, been filmed more than a dozen times, and more than 50 million copies of Heidi books have been sold world-wide (Switzerland's population is only 7 million ...).
Summary of Johanna Spyri's Heidi Story
The orphan child Heidi first lives with her aunt Dete, but Dete would like to concentrate on her career. So she brings Heidi to her grandfather, a queer old man living in an alpine cottage far from the next village (he is therefore called Alm-Uncle, Alpöhi or Almöhi in German). Alm-Uncle is good-hearted but mistrusts anybody and wants to keep the child from all evils of the world. So he refuses to send Heidi to school; instead she goes to the pastures, together with Peter, a shepherd boy looking after the goats (Geissenpeter = goat-Peter in German). This (all too harmonious) apine idyll finds a sudden end when aunt Dete comes in again and brings Heidi to Frankfurt (Germany) where she shall stay with Clara, the paralyzed daughter of a rich family, and learn something.
Thanks to the grandmother of Clara, Heidi learns to read but she can't get acquainted to the strict discipline in a bourgeois upper class house (personified by governess Fraulein Rottenmeier). She is very lonesome and gets depressed by the gray anonymous city. Heidi becomes ill of homesickness, she starts to walk in her sleep. Miss Rottenmeier is alarmed, not because of the fate of the poor child, but rather because she thinks that there are ghosts in the old house. Finally Clara's father Herr Stresemann and the sympathetic doctor of the family decide to stay up till midnight and find out about the ghosts. When the doctor sees Heidi walking around in her sleep, he finds the right diagnosis and sends her back to the alps.
Next summer, Clara visits Heidi there. They go to the pastures and Heidi shows Clara all the beauty of her world. Peter gets terribly jealous, and in a moment when he feels unobserved, he pushes the empty wheelchair down to the valley so it gets smashed. Clara wants to see the flowers and is forced to walk - and her desire is strong enough that she overcomes her handicap. Healings at body, spirit and soul in that healthy Alpine world - end well, all well.
Heidi at the Cinema
Heidi Movies from 1920 to 2001
|The first film production on Heidi was a silent movie made in USA
|Sound film by Allan Dawn starring Shirley Temple as Heidi
|Swiss film production by Luigi Comencini starring Elsbeth Sigmund as Heidi, Thomas Klameth as Geissenpeter and Heinrich Gretler (one of Switzerland's best know actors) as Alpöhi. This was probably the most successful Swiss movie in the USA (300 copies in 4300 movie theaters).
|Franz Schnyder tries to follow on his own successful 1954/1955 film productions dealing with stories by Jeremias Gotthelf (a conservative early 19th century Swiss writer) and presents his own version of Heidi (same leading actors as in Comencini's production). Schnyder's Heidi is the first color movie produced in Switzerland - (all too) obviously in the service of tourism-advertising
|Austrian film production starring Eva Maria Sieghammer as Heidi
|American production starring Jennifer Edwards as Heidy
|Japanese animated cartoon films (a television series consisting of 52 issues), very successful also in Germany, Austria and Switzerland
|German televion series (26 issues) starring Katja Polletin (Austria) as Heidi, Stefan Arpagaus (Switzerland) as Geissenpeter and Katharina Böhm as Clara
|Alienation of the subject matter: Michael Douglas produces Courage Mountain in Austria. Heidi and Peter are presented as a young courting couple during World War I
|Short television series by Walt Disney
|The recent Swiss Heidi film production by Markus Imboden keeps roughly to the traditional action frame, but modernizes the details radically - concerning both the psychology of the figures (the stubborn Geissenpeter has changed into a cool boy) and the hightech accessories (Heidi and Peter communicate via internet and mobile phone SMS [short message service, very popular among European teenagers]).
|Remake of the successful Japanese comic series of 1977
At first sight, Heidi is certainly «an emotional story dealing with primitive fears; the anxiety of the child to be without parents and to be displaced.» (Markus Imboden, movie director). To this substance the story is reduced essentially when Imboden starts his 2001 Heidi movie on the Alp (not on lovely pastures, but rather in a modern mountain restaurant, however), and finishes at a Popmusic concert in Zurich's Hall Stadium. Imboden admits openly that he intends to radically change the view we have of Heidi, «because it has been misused, instrumentalized ideologically and politically.» With this kind of internal distance to the traditional view of Heidi, one might, however, miss the chance to understand this novel for children in a deeper sense - taking into account that all good children's books have to say quite something to adults as well.
Perhaps we have to keep a little distance from the tendency of Swiss intellectuals to critize traditional Swiss values and take a really detached view - for example a Japanese view. While some academics in Switzerland are very critical about Johanna Spyri in general and her novel Heidi in particular, it may be significant that the only complete edition of Johanna Spyri's works was published in Japan (1962). As far as has been reported in Swiss newspapers, young Japanese women do not see and love Heidi as a precocious, vigourous teenager (as Imhof presents her). Heidi seems to be loved as a child of nature, a symbol for romanticism and lost innocence.
Such notions, however, remind of the end of the 19th century when authoress Johanna Spyri coming from a peasant village Hirzel am Albis lived in Zurich and shared the feelings of insecurity with thousands of workers displaced by the compulsions of the industrialisation. Moving out of rural Switzerland into modern cities was sort of a "cultural shock" for most of them. Besides they had to struggle hard to find their place, not only economically, but as well emotionally.
Johanna Spyri's Heidi-story tries to give orientation in a world shaken by rapid social change, a world in disorder that makes people feel insecure - and this is exactly what makes the story attractive today in view of neoliberalism and globalization.
The foundation on an inctact alpine nature is, however, but one element of the story and one should not have an isolated view on it. Heidi as presented by Johanna Spyri does not return into the intact alpine world as if she had never been to the metropolitan city - to the contrary: Heidi makes use of what she has learnt (the title of the second book is program!). Johanna Spyri does not opt for a cheap retreat into a idealized sweet world (as grandfather Almöhi does in his frustration about mankind), neither for an obstinate keeping to simple views (as Geissenpeter, who is too lazy to learn reading and writing). To the contrary: Johanna Spyri wants to empower people to accept new challenges while keeping a good heart like Heidi, who is able to read stories to Peter's blind grandmother and even moves her unsociable grandfather to return into the village community.
Seen from this perspective, I wouldn't interpret the message of the story as sheer propaganda for internet and mobile phone "literacy" (on a simply technical level) neither for coolness as Markus Imhoof's movie might suggest. The real challenge today is not, whether to use the modern means of communication at all, but rather what to communicate.
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Virginia Highlands Community CollegeHome Menu
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College is a time to explore life, to connect with new friends, to grow personally and academically, and to learn about yourself and the world around you. Excel at Virginia Highlands Community College helps students make the most of their college years by providing specialized services delivered by a staff of experts.
We're here in the beginning to help you choose a program of study and apply for financial assistance. We're here when you need help balancing the pressures of life and meeting the demands of the classroom. And we're here when you're ready to make the next step and need help transferring to a four-year college or university.
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Finally some good budget news -- just a little anyway. With the thaw of the recession comes a welcome surprise for state coffers.
According to the state controller's office, California is running about $4 billion ahead of projected personal income tax revenues for 2010-11. That's important because personal income taxes represent slightly more than half of all state generated receipts. Sales taxes, which account for about 25 percent of all revenues, are also running slightly ahead of projections.
This news is important as state leaders try to find a way to balance the budget. Democratic Governor Jerry Brown has advocated a public vote on extending three temporary tax increases on income, sales and motor vehicles for five years.
For the record, corporate income taxes, which contribute about one-eighth of state revenues, are running slightly behind projections. The remaining revenues come from various fees and special taxes. Bottom line: for the moment, California looks to be collecting about $4 billion more than anticipated.
Republican leaders in the state legislature have argued that the budget must be balanced via cuts beyond the $14 billion that have already been sliced out of the budget, more than half of which have come from K-12 public education, community colleges and state universities.
So, now what?
State leaders may have a bit of wiggle room. This may allow them to agree on a plan for the voters that keeps the temporary taxes for only three years instead of five. Or perhaps they will ask the voters to decide on only two of the three temporary taxes instead of the current three. The point is that the unexpected revenues may give leaders flexibility they have not had previously.
That may help us through the current mess, but it's the long term issues that elected officials must address. Every year, California operates with a $20 billion structural deficit--that is we begin the year with commitments of $20 billion more than we take in. Sooner or later, leaders will have to agree on permanent reductions or new revenue sources.
All this can change, of course, between now and the end of the fiscal year on June 30. But if the trend continues, California's $12 billion projected deficit could be sliced by one-third--for this year, anyway.
For now, they may find it just a little easier to sleep at night, even if the respite is temporary.
State Finds Extra $4 Billion
Finally some good budget news -- just a little anyway. With the thaw of the recession comes a welcome surprise for state coffers.


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As the investigation into the Dubai assassination of a Hamas official expands abroad and pushes Israel further on the back foot, the man who set it in motion, Dubai's outspoken police chief, has gone surprisingly quiet.
He made no comment Tuesday after Britain offered the first outside confirmation of his claim that Israel was involved in the Jan. 19 killing of Mahmoud al-Mabhouh. Foreign Secretary David Miliband accused Israel of forging British passports used by the hit-squad suspects and expelled an Israeli diplomat in response.
The silence marks a change for Lt. Gen. Dahi Khalfan Tamim, the stern police chief with thick-rimmed glasses who seized the spotlight as it swung toward him over the assassination. He stretched 15 minutes of fame into weeks of international headlines, holding press audiences with impressive evidence and insulting zingers at Israel. "Even the disguise was primitive," he told reporters. "If they want a training course in disguise, we would be happy to oblige."
Tamim is famous here in the United Arab Emirates (UAE) as a rare government official who speaks freely. Emiratis say he's earned the clout to say what he likes by being good at his job, which he has held since 1980, longer than all of his predecessors combined.
Why the chief is holding his tongue now remains unclear. Did he grow too feisty and get silenced by the UAE's ruling families? Is it all part of the plan? In either case, the latest development from Britain furthers both of his apparent goals: to continue the investigation and to keep the heat on Israel.
Australia, France, Germany, and Ireland, who also had forged passports used by suspects in the killing, have launched inquiries as well.
A serious cop
The Mabhouh murder is not the first high-profile case that Tamim has overseen. In 2007, he nabbed members of the Pink Panthers international crime gang after they rammed their cars into a mall and sped off with $4 million worth of jewelry. Tamim vowed to identify the thieves within a week – drawing skepticism – and did. Three suspects were soon arrested. Others were later caught in Europe with the help of Interpol and other agencies.
Locals felt so proud at the time that they created a Facebook fan page for "Dubai's one of a kind" police commander.
"I didn't think it could happen, he did it and well this is in honour of the Chief and his force," the creator of the webpage wrote in a post.
Tamim's reputation soared again after he unraveled two prominent murders, one a famous Lebanese pop singer in 2008 and another, a Chechen dissident a year later.
"If anything happens in Dubai, he can catch it," says Sheikha Al Suweidi, a housewife from Abu Dhabi.
Nearly a dozen Emiratis and Arabs interviewed all knew of Tamim (they call him Dahi Khalfan) and spoke of him with pride.
Beyond solving sensational cases, Tamim has built a reputation as someone who's kept Dubai safe for 30 years, even as it transformed into a glitzy cosmopolis hosting some 200 nationalities and 7 million visitors a year.
"Everybody gives him credit for the fact that Dubai is one of the safest cities, given how open it is," says Abdulkhaleq Abdulla, a political science professor at Emirates University in Al Ain.
Evenas Dubai changed at breathtaking speed, he kept pace. He introduced a state-of-the-art forensics lab and blanketed the city with video surveillance, with estimates ranging from 1,500 to 6,000 cameras. Officers received training from the United States Federal Bureau of Investigation and other leading agencies.
Other measures went further. According to security analysts, informants are plucked from the various nationalities here to spy on their communities. E-mails and phone calls are tapped at will. Petty thieves are deported, but not before having their eyes scanned for a biometric database to blacklist them from ever returning.
Foreigners here who know nothing about the police chief attest to his success.
Dubai is "way safer" than cities in Britain or America, says a banker who moved here from London two years ago and asked that his name not be used, echoing a sentiment widely shared among Westerners. "I feel no fear of anything," from walking around late at night to trusting that lost belongings will be returned.
According to a 2009 survey conducted by YouGov for the UAE-based paper The National, 96 percent of Dubai residents said they felt very safe or somewhat safe in the emirate.
That sense of security has contributed to Dubai's ability to draw tourists and investors at least as much as any of the luxury hotels, indoor ski slopes or beach-side restaurants that have put it on the map.
The reluctance of the Brit and others to be identified represents the flip side of Dubai's "openness" and security. The press is controlled, and speaking out on sensitive topics can be a way for foreigners, whether bankers or taxi drivers – to lose their visa.
An insider, but outspoken
Tamim's success has earned him a place in the inner circle of Dubai's ruler, Sheikh Mohammed bin Rashid Al Maktoum. As head of security, Tamim sits on the the sheikh's Executive Council. He also chairs the budget committee.
Tamim is "well trusted by Sheikh Mohammed," says Professor Abdulla. "He's one of the longest-living lieutenants next to the sheikh," and stands with him in the core group of "people who made Dubai."
Both men, born two years apart, started their careers in the police force. In 1968, Sheikh Mohammed was named the head of Dubai police, a title he still holds, and two years later Tamim graduated from the Royal Police Academy in Jordan and joined the force.
Tamim's trusted status has given him license to speak openly about sensitive subjects. In 2002, he became the first public official in Dubai to admit that prostitution exists here.
He has also fanned the controversy of Emiratis feeling lost in their own country as the expat population swells. At the height of Dubai's construction boom and foreign influx in 2008, he criticized both policies as a threat to Emiratis, urging that the number of outsiders be capped and that locals have more children. "I'm afraid we are building towers but losing the Emirates," he warned.
"He's able to say stuff that's blatantly contradictory," says Jim Krane, author of City of Gold: Dubai and the Dream of Capitalism. "Others get fired for much less."
Last year, as the financial crisis sank one company after another, Tamim threatened a police boycott against any firm that laid off Emiratis "under the pretext of the current economic meltdown." The warning was seen as a challenge to a major local firm, Al Futtaim, which had recently dismissed 20 locals.
Anger, then silence
Tamim fired off similar tough talk as the Mabhouh investigation intensified. It was the biggest murder case to ever hit Dubai, and was seen as a brazen attack on the emirate's openness. It came at a stressful time for the chief. The financial crisis had dealt the emirate a major blow. With jobs being cut and wages squeezed, crime was on the rise. Tamim's mother, whom he used to visit in the mornings before work, passed away in early February just as the case was heating up.
Tamim's studious presentation of evidence gave way to outlandish threats. He had debuted with a stunning 27-minute video following suspects from their arrival at the airport to the hotel where they killed Mabhouh. He had released the photos and information from the forged passports as well as a detailed chart showing when the suspects entered and exited the country.
Then he vowed to ban Israelis from entering Dubai on second passports, raising eyebrows about racial profiling by saying they would be identified by their accents and faces. He called for the arrest of Israeli Prime Minister Benjamin Netanyahu. He ordered spies across the Gulf region to leave "or face extreme measures." He challenged Israel's spy agency Mossad to hand over its spies for DNA testing and vowed to resign if the identities didn't match.
At the same time, behind the scenes, Tamim was sharing additional evidence with Interpol, prompting it to issue arrest notices for all 27 suspects and join an international task force in the investigation.
Then, Tamim went silent.
He may be working with Interpol and other countries as the investigation runs its natural course outside Dubai. He may be holding his fire to secure a promise from Israel that this won't happen again. (Because the UAE lacks diplomatic relations with Israel, public pressure and private talks are its main tools for dealing with the country.)
He may have been asked to tone it down. A few days before Tamim disappeared from center stage, he received a visit from Qatar, one of the few Arab states that recognize Israel, sparking rumors that it wanted him to stop making a scene.
But many analysts, and certainly his fans, don't think he should feel embarrassed.
Dubai police have "blown a lid on government intelligence hits. I don't think anybody's going to do this in Dubai anymore unless they really don't" care about being caught, says Mr. Krane.
A Saudi who has lived in Dubai for three years says that, despite the empty threats, Tamim will come out on top. "Even if he doesn't achieve [the arrest of Prime Minister Benjamin Netanyahu], it won't make him look bad. He's already made Israel look bad.
"He's a professional," continued the Saudi, who asked not to be identified. "He's been doing this a long time."


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UH Hilo to resume business on August 16; Orientation Week activities rescheduledUniversity of Hawaiʻi at Hilo
University of Hawaii at Hilo
HILO, Hawaii - University of Hawaiʻi at Hilo officials today announced that the campus will be in full operation beginning Thursday, August 16, 2007. UH Hilo and Hawaiʻi Community College closed on August 14 and 15 due to Hurricane Flossie.Several offices at UH Hilo are currently open, however, to assist students participating in Orientation Week. The Admissions Office is open for advising, and the Student Health Center is open. Both offices will remain open until 4 p.m. today. Also, students who still need to register for fall classes may do so today until 4 p.m. in UCB 103, 104 and 105 computer labs. For Orientation Week schedule changes, please note the following taking place on Thursday, August 16:* Math Placement Exam (added test time): 9 a.m.- 3 p.m., UCB 101 & 102
* General Advising Sessions (general education, graduation requirements, transfer issues): 10-11:15 a.m. for new students, UCB 114; 10-11:15 a.m. for transfer/returning students , UCB 112; 2-3 p.m. for new, transfer, returning students, UCB 100
* Peer Assistant Linkages & Support (PALS) Orientation & Advising: 11:30 a.m.- 2:30 p.m., Campus Center 301
* Luʻau Under the Stars: meet at Campus Center turn-around at 2:45 p.m. for shuttles to ʻImiloa Astronomy Center tour; luʻau will be held in the Campus Center Dining Room
For additional information and updates regarding orientation, please call (808) 933-0732 or visit http://www.uhh.hawaii.edu/orientation.