there  will  be  seen  a  beautiful  green  Hiinrescencc,  and  the  atisDrjHion
bands  of  alkaline  urobilin  may  be  found.  This  spectrum  is  cliar-
acteristic.  That  of  acid  urobilin  may  he  determined  in  a  urine
directly  if  a  few  drops  nf  a  mineral  acid  be  added,  but  it  is  belter  to
shake  out  with  aniyl  alcohol  and  e.\amine  the  extract.  Or  to  the
urine  may  \k  aiUlcd  an  e'jual  amount  of  lo  per  cent.  ZnAc  in  absolute
alcohol,  and  the  mixtnre  filtered.'"  Tliis  lest  is  given  even  in  the
presence  of  considerable  bilirubin.  The  fluorescence  is  best  seen
with  a  convex  lens,  which  gives  a  luminous  green  circle.
For  i|uaniiu<iv«  wo^b.  Hoppc-Seyler'*  meilioj  i^  recomcncntled.    One  hundred
of  urine  ar«  acidulated  vdth  ftulphurk  acid,  snitirated  with  Hmmonium  ^^iilphaie.
t*  Cotnpi.-rend,  Soc.  de  Biol..  54.  p.  7155,
•CenlTalW.  f.  inn.  Med.,  1993.  vol,  xxiv.  p.  ill.
'  See  alM)  Rolle'ion'i  ca'c  of  urnbihn  iaundice  (allowing  trional  in  a  «»e  with
nuimes  liver.  Brit.  MciL  Jour.,  i&)7.  i,  p  719.
'Eflwii.  P»af,  ined,  Wochcnschr,  IQ04.
"  Sthlt.  inger,  Deulicb.  raed.  Wochenschr..  1503.  No,  32.  p.  561,


93  CLINICAL   DIAGNOSIS

and  allowed  to  stand  for  some  time ;  then  filtered,  and  the  precipitate  washed  with
saturated  ammonium  sulphate.  The  precipitate  is  then  pressed  out  between
blotting--paper,  extracted  with  equal  parts  o£  alcohol  and  chloroform  repeatedly.
The  extract  is  then  filtered  into  a  separating  funnel,  and  to  the  filtrate  is  added
two  volumes  of  water  and  then  chloroform  until  the  chloroform  settles  out  well
in  a  clear  layer.  The  chloroform  solution  is  evaporated  on  a  waler-bath  and  the
residue  dried  at  ioo°  C.  It  is  then  extracted  with  ether,  the  ether  extract  filtered
off.  the  residue  dissolved  on  the  paper  in  alcohol,  again  brought  into  the  weighed
beaker,  evaporated,  dried,  and  weighed.
