DUCLAUX:  "Effect  of  presence  of  microbes
upon  germination."  Meeting  of  Acad.  of  Sci.,
Paris,  Jan.  5,  1885.
BACTERIA    INVESTIGATION.  237

RALPH  (T.  S.)  :  "Living  Bacilli  in  the  cells  of
Vallisneria  and  Anacharis."  Journal  of  Mi-
croscopy, iii.  (1884),  p.  17. —  Proc.  Roy.  Soc.
Victoria,  10  May,  1883.
REINKE  u.  BERTHOLD  :  (Plant  pathology).  Un-
tersuchungen  aus  dem  botan.  Laboratorium  in
Gottingen.  Hft.  I.
WALKER  :  "  Yellow  disease  of  Hyacinths."  Bo-
tan.  Centralblatt.  Bd.  14,  p.  315.
BACTERIA  INVESTIGATION.
PART  III.
FORMULARY.
1.  BERGMANN'S  OR  BUCHOLTZ'S  FLUID.
Distilled  water 100      ccm.
White  rock  candy 10        "

Tartrate  of  ammonia 1        **

Phosphate  of  lime 0.5     "

2.  BISMARCK-BROWN.
This  takes  the  lead  of  all  aniline  dyes,  in  that  it
is  not  so  easily  washed  out.  The  preparations  may
also  be  mounted  in  glycerine,  which  is  not  the
case  with  other  aniline  dyes.  It  is  also  especially
adapted  to  photography.  Weigert  makes  a  satu-
rated solution  with  boiling  distilled  water,  or  one
in  which  there  is  very  little  alcohol ;  after  cooling,
filter,  set  aside,  and  allow  to  settle.  After  stain-
ing, wash  the  sections  for  a  short  time  in  absolute
alcohol.  Whole  pieces  may  be  stained  at  once
before  cutting  by  using  an  alcoholic  solution  of
Bismarck-brown.  Baumgarten  used  a  concentrated
(one  per  cent.)  acetic  acid  solution.
BACTERIA   INVESTIGATION.  239

3.  GLYCERINE  ANILINE-BROWN.
Used  by  Koch  for  photographing.  Make  a
concentrated  solution  of  aniline-brown  in  equal
parts  of  glycerine  and  water.  Filter  from  time
to  time.
4.  ANILINE  OIL.
