The prepared, wet sediment may be stored (for further use in the culture only) at 4 ± 2 °C in the dark for a period of 2 to 4 weeks from the day of preparation (8).
Development and validation of a homologous zebrafish (Danio rerio Hamilton- Buchanan) vitellogenin enzyme- linked immunosorbent assay (ELISA) and its application for studies on estrogenic chemicals.