The spiked sediments prepared according to paragraph 16b are placed in the vessels and crystallising dish and overlying water is added to produce a sediment-water volume ratio of 1:4.
Shinomiya,A., H.Otake, K.Togashi, S.Hamaguchi, and M.Sakaizumi (2004), “Field survey of sex- reversals in the medaka, Oryzias latipes: genotypic sexing of wild populations”, Zoological Science 21, pp. 613-619.