Alternatively, samples may be pooled in RNA stabilization reagents, such as 0,2 g tissue/ml reagent according to the recommendation from the manufacturers, although each fish shall be processed individually and shall not be pooled in the samples because of the small amount of material to be used for extraction.
Le traitement de tous les inoculums par le sérum anti-virus de la NPI vise à empêcher l'effet cytopathogène (ECP) dû au virus de la NPI de se manifester dans les cultures cellulaires inoculées.