[Cloning of the recombinant human hepatocyte growth factor gene].
The full length of HGF cDNA gene(2,184 bp) was amplified successfully from
human liver cDNA library by RT-PCR. The recombinant plasmid pGEM-HGF was constructed and confirmed by restriction enzyme digestion and DNA sequencing. The recombinant plasmid pGEM-HGF was transfected into COS-7 cells by lipofectamine. The expression of HGF was detected by Western blot and ELISA