Selection for improved protein stability by phage display.
A library of mutants of a single-chain Fv fragment (scFv) was generated by a combination
of error-prone PCR and phage display. The library was screened for improved stability of the scFv in the presence of human serum. The most stable mutants were selected and characterized. The most stable mutants were found to be those with the highest affinity for the antigen. The most stable mutants were also found to be