Comparison of spontaneous and contraceptive menstrual cycles on a visual discrimination task. 21 women with spontaneous ovulatory menstrual cycles, and 7 women who were regular users of oral contraceptives were investigated over two consecutive menstrual cycles. There two groups were compared on their two flash threshold performance and on mood ratings. It was found that, for women with ovulatory menstrual cycles, visual sensitivity was enhanced during the late follicular phase of the cycle, as ovulation approached. At other phases of the menstrual cycle (paramenstrual and luteal) visual sensitivity remained constant and comparable to the values found in women who were taking to the values found in women who were taking contraceptives. Women who were taking contraceptives showed no significant variation in visual sensitivity with phase of the menstrual cycle. The overall level of anxiety, as reflected by mood ratings was higher in women with spontaneous cycles compared to those taking contraceptives. Neither group, however, showed significant variation in anxiety with phase of the cycle.


A prospective study of premenstrual tension symptoms in healthy young Australians. Thirty healthy young women volunteered to complete questionnaires concerning physical and psychological symptoms during a full menstrual cycle. A maximal incidence of minor physical and psychological symptoms was observed in the first few days of menstruation. This was preceded by a gradual rise in the level of symptomatology during the premenstruum.


Mothers and children in hospital.

Mothers and children in hospital. Between 1965-1972 244 individual mothers were admitted on 283 occasions accompanied by 287 children to a general hospital unit. This cohort has been followed up to ascertain re-admission to any New Zealand psychiatric facility for two follow-ups at 1-8 years and 5-12 years. A postal survey of attitudes was also carried out. The content of the replies has been analysed, with statistical and anecdotal accounts of patients' and husbands' expressed feelings and attitudes. These are compared with observations in the literature about themes of hostility and anxiety. The circumstances surrounding the homicide of a baby in this series together with our various rationalisations, value judgements and assessment of the situations are described.


Family structure and relationships of 50 female anorexia nervosa patients. Family features of the first 50 anorexia nervosa patients referred to a psychiatrist from a defined geographic area are documented. Information about the families was obtained from personal interviews with the parents during the course of the patients' treatment. High socio-economic class, absence of non-white population, older age of parents, instability factors in the parents' backgrounds and high incidence of both physical and psychiatric illness in the parents suggest multiple factors may be aetiological. Possible pathogenic factors are equally distributed between mothers and fathers.


Marital interaction in depression.

Marital interaction in depression. The great majority of depressed patients are currently married or formerly married and depression is commonest at ages when marital satisfaction is least. Study of patterns of marital interaction in depression may provide useful leads for understanding the aetiology and therapy of depressive syndromes. Theoretical views of depression as loss, as coerced behaviour and as coercive behaviour suggest useful hypotheses. Both marital interaction and depression are complex variables to define and measure. A literature review suggests reduced general affective involvement, reduced expressions of affection, increased criticism, increased husband domination and a marked struggle for interpersonal control in the marriages of depressed persons. Marital therapy may be the most useful psychotherapeutic approach for depression.


Urban-rural differences in student mental health: the Hong Kong scene. Student mental health of Hong Kong urban and rural fifth formers and sixth-formers was assessed by examining their scores obtained from the General Health Questionnaire twice administered. The first administration was done soon after commencement of a school term and served as a baseline measure, and the second was done six weeks before the fifth-formers took an important public examination, a significantly stressful event. The results indicated that all groups of fifth-formers showed an increase in mean scores, reaching statistical significance only in urban boys and rural girls. The latter finding was used to explain urban-rural differences in mean scores. The significance of severe examination distress and its possible late psychiatric sequelae remained unanswered.


Posted summaries of marital therapy.

Posted summaries of marital therapy. Summaries of marital therapy sessions, similar to those used in group psychotherapy by Yalom, were posted to reach couples three or four days after each session. The summaries of 600 to 4,000 words included a narrative account of the session and some editorial comment by the therapist. Couples completed a questionnaire assessing the use of the summaries. Replies to questionnaires and clinical impressions indicate the summaries had good acceptability. Couples felt the summaries increased their focus on marital problems between the sessions and provided helpful clarification.


Time--a neglected aspect of psychiatric treatments. This review attempts to deal with the complex issues involved in the time to heal, with special reference to psychological processes. The questions of convalescence and relapse in organic medicine are explored and extrapolated to psychiatric processes. The concept of a latency period of change in treatment outcome is discussed with reference to both less complicated reactive states as well as highly charged neurotic processes. The problems of recognizing slow but perceptible change and separating it from failure to respond is analysed. The value of long-term psychotherapy is assessed and comparison made with the newer concept of short-term therapy. Crisis therapy and disaster reactions are discussed within the concept of time to heal. Finally the difficult issue of "miraculous cure" with its therapeutic implications is evaluated.


Personality of a group of grossly obese women. The current view that the morbidly obese do not show a unique personality pattern is challenged. A sample of 91 obese female patients at a general practice are identified as having a significant personality pattern using the Sixteen Personality Factors Questionnaire (16 PF) and the Neuroticism Scale Questionnaire (N.S.Q.). Further the personality factors are consistent with a model that explains aspects of morbid exogenous obesity.


The Ph1 chromosome in childhood leukaemia. Ph1 positive leukaemia was diagnosed in eight of 123 (6.5%) consecutive new cases of childhood leukaemia. Four patients presented as typical chronic granulocytic leukaemia (CGL) and four as acute leukaemia, two of the four children with CGL have since developed blast crisis. Morphological and immunological characterization of the blasts in these six acute cases was suggestive of a lymphoid crisis in three, a mixed crisis in two, and a myeloid crisis in one. Remission was achieved in five of the six patients but lasted more than a year in only two; in one of these two patients the blasts at the time of crisis lacked the Ph1 chromosome and in the other, who presented in myeloid crisis, remission ended in a lymphoid crisis. Cytogenetic studies showed unusual translocations in two of the eight children. Serial examination showed that three children the proportion of Ph1 positive cells in the marrow was reduced to less than 6% at the time of remission from acute leukaemia. We conclude that Ph1 positive leukaemia in childhood presents frequently as acute leukaemia and may even mimic 'good risk' acute lymphoblastic leukaemia. The response to treatment cannot be predicted by morphological or immunological characterization of blast cells. Storage of bone marrow for possible autologous transplantation should be considered in children who achieve remission.


Y chromosome loss in childhood leukaemias. During the past 3 1/2 years cytogenetic studies have been performed on all children with leukaemia prior to treatment. Loss of the Y chromosome documented by chromosome banding techniques, was observed in bone marrow blast cells from two of II consecutive male children. On patient had acute myeloblastic leukaemia of t(8;21) type. The other patient was diagnosed as acute lymphoblastic leukemia. Our studies provide evidence that Y loss occurs in childhood leukemias and is not necessarily related to an ageing process as suggested by others. Futhermore, this study demonstrates that Y loss can occur as a sole abnormality in non-myeloid leukaemia.


Defective hydrogen peroxide production in chronic granulocytic leukaemia neutrophils. Hydrogen peroxide (H2O2) production by polymorphonuclear neutrophils (PMN) during phagocytosis was fluorometrically vestimated over an incubation period of 80 min during which dead Candida guilliermondii were ingested. Ten patients with chronic granulocytic leukaemia (CGL) were tested and compared with 15 normal subjects. The production of H2O2 by CGL neutrophils was about 29% of normal. There was no correlation between H2O2 production and the impaired killing of Candida guilliermondii by the neutrophils of CGL patients.


Infiltration of central nervous system in adult acute myeloid leukaemia. Out of 64 consecutive unselected patients with acute myeloid leukaemia studied during 1973-6, five developed clinical evidence of spread to the central nervous system (CNS). Neuroradiological examination showed cerebral deposits in three, in whom rapid symptomatic relief was obtained with radiotherapy. In two of these patients who developed solid intracranial deposits haematological remission could be reinduced or maintained; they were still alive 86 and 134 weeks later. When patients presented with spread to the CNS complicating generalised uncontrolled leukaemia they had short survivals. CNS infiltration may respond dramatically to appropriate treatment provided that it is not associated with generalised uncontrolled leukaemia, which has a poor prognosis. In view of this, routine "prophylaxis" of the CNS in adult acute myeloid leukaemia does not seem justified at present.


Evolution of karyotypes in Philadelphia (Ph1) chromosome-negative chronic myelogenous leukemia. Ten of 55 patients with chronic myelogenous leukemia (CML) diagnosed between 1972 and 1977 were found to lack the Philadelphia (Ph1) chromosome. Serial clinical, morphologic, and cytogenetic studies of patients with Ph1-negative CML showed that 30% of them had chromosomal abnormalities. Two had an extra chromosome No. 8 at the time of blast crisis, with a morphological picture of myeloblasts in the bone marrow. A third patient had a 6:14 translocation initially Abnormalities of chromosome No. 14 are frequently seen in lymphoproliferative disorders, and the bone marrow and peripheral blood contained a significant population of lymphoblasts as well as myeloblasts. The median survival for the 10 patients was 19 months. The exact nature of Ph1-negative CML is not yet clear; disease appears to be a distinct entity among the myeloproliferative disorders.


Relation of "lymphoid" phenotype and response to chemotherapy incorporating vincristine-prednisolone in the acute phase of Ph1 positive leukemia. Forty-four patients with Ph positive leukemia (36 developing blast crisis after chronic phase and eight presenting in acute leukemia) were classified into subgroups on the basis of reactivity of blasts with an anti-serum made against non-T,non-B acute lymphoid leukemia (ALL+), levels of terminal transferase enzyme (TdT+) and morphology. Positivity with anti-ALL serum was the most sensitive and reliable marker, and TdT was an important aid. The presence of "lymphoid" blasts in blast crisis of CML was related to the response to chemotherapy incorporating Vincristine and Prednisolone (VP). Patients with ALL+ blasts frequently (14 of 15 cases) responded to therapy while 21 of 25 patients who had no ALL+ blasts failed to respond. The clinical course of the ALL+ patients was variable: eight patients remitted with return to the appearances of the chronic phase; four patients demonstrated elimination of the Ph1 positive clone with hypoplasia and this was followed by normal (Ph1 negative) marrow regeneration in two. Subsequent relapse was of either the ALL+ "lymphoid" or the ALL-myeloid type. A regimen incorporating VP should be the treatment of choice in "lymphoid" blast crisis of CML.


Acute lymphoblastic leukemia: the significance of nuclear convolutions. Because of the significance attributed by some investigators to the presence of cells with convoluted nuclei in lymphoblastic lymphoma, and the absence of any information on the presence and significance of such cells in acute lymphoblastic leukemia (ALL), we reviewed the blood, bone marrow films, and clinical records of 101 children with ALL. We attempted to determine whether leukemic cells with convoluted nuclei can be recognized in such films, and if so, whether this observation has clinical significance. In fifty-seven of the patients the leukemic cells had convoluted nuclei. For the purposes of this study, our patients were divided into three groups: Group I, 44 patients without cells having convoluted nuclei: Group II, 30 patients with 10% or fewer convoluted nucleus cells (CNC); and Group III, 27 patients with more than 10% CNC. Clinical comparison of the three groups with respect to age and sex distribution, physical and hematologic findings at presentation, response to therapy and survival showed no significant differences. Patients with a mediastinal mass had a significantly shorter survival compared to those without a mediastinal mass, regardless of the presence or absence of CNC (p = 0.0001). Our results indicate that the nuclear convolutions can easily be recognized in blood or bone marrow films of patients with ALL, and that their presence has no provable clinical significance.


Chromosome abnormalities in acute promyelocytic leukemia (APL). Sixteen patients, 15 adults and one child, with APL have been studied cytogenetically; 14 of these had an abnormal karyotype (87%). Eleven of these consistently showed a t(15;17)(q26;q22) structural anomaly, one patient showed a 47,+8 karyotype, one a rearrangement of chromosomes No. 15 and No. 17, apparently different from that in the other patients, and one a No. 17 deletion without a demonstrable translocation. as an additional chromosome change trisomy No. 8 was found in 5 cases and monosomy No. 7 in two. The t)15;17)(q26;q22) structural anomaly is highly characteristic of APL, is found in APL of children and adults, but it is apparently not associated with a clinically different form of APL.


Typhlitis in acute leukemia: successful treatment by early surgical intervention. Inflammation of the cecum ("typhlitis") has been an unusual, but generally fatal complication of severe granulocytopenia and immunosuppression, occurring during the therapy of hematological malignancies. The diagnosis has usually been made only at autopsy, and early surgical intervention has often been withheld because of the patient's precarious hematological status. We report here a patient in whom the clinical diagnosis of typhlitis led to early operation, with intensive blood component support. The successful outcome suggests that such an approach might improve the usually grim prognosis in patients whose underlying malignancy offers a clear chance for remission.


Metastatic osteogenic sarcoma to the brain. The radiographic appearances of intracranial metastases from primary osteogenic sarcomas in three patients are presented. In two patients, the diagnosis of metastatic disease could be made on conventional roentgenograms of the skull since mineralization of tumor osteoid could be seen within the brain parenchyma. This had a similar appearance to the primary bone tumor. Angiography in two of the three patients revealed the cerebral metastases to be hypervascular with tumor vessels, tumor stain and early draining veins.


Management of asparaginase induced hemorrhagic pancreatitis complicated by pseudocyst. Asparaginase induced hemorrhagic pancreatitis is a rare but serious development occurring in less than 0.5% of patients treated with this drug. Severe pancreatitis with progressive abdominal distention, toxemia, hypotension and respiratory insufficiency occurred in an 18-year-old patient with acute lymphoblastic leukemia following treatment with asparaginase. There was a dramatic response to high flow peritoneal lavage with rapid recovery within 24 hours from a moribund state. The subsequent development of a pseudocyst, with progressive increase in size and development of obstructive symptoms, required surgical decompression. Transgastric cystogastrostomy was successfully carried out.


Neutropenic enterocolitis: two case reports of long-term survival following surgery. We report two cases of neutropenic enterocolitis with long-term survival following surgery. This favorable outcome was related to three major factors: recognition of the acute surgical abdomen, appropriately timed surgical intervention, and a prompt postoperative return of normal circulating white cells.


Palmar flexion creases in childhood neoplasia. About 90% of Caucasian individuals in the general population may observe two transverse palmar flexion creases when their fingers are slightly bent. A small minority may find in one hand a single transverse crease or the usual two creases, of which one seems to cut across the palm to the ulnar margin. Those unusual creases are called simian- or Sydney-creases, respectively. Normal healthy people hardly ever observe them in both hands. We observed those unusual creases, however, in more than 50% of children suffering from different type of malignant neoplasia, quite often in both hands. The difference between patients and controls of similar ages is highly significant. Among patient with childhood acute lymphocytic leukemia the variant creases were mostly of the Sydney type. They most frequently occurred in those patients in whom the disease had become manifest at an early age. Since fathers as well as mothers of the patients showed significantly higher frequencies of unusual creases, the phenomenon seems to be a familial one. A most singular effect is the striking incidence of those creases in younger siblings of the patients. With regard to crease variance our data are essentially similar for lymphoproliferative disorders (ALL and NHML) and embryonic malignant tumors. This might be the first indication of a common host factor in patients with ALL or embryonic tumors occurring in early childhood. We have postulated that this factor may be a regulatory one associated with cellular growth and differentiation in early fetal palmar pads as well as with cell-mediated immune response to early pediatric tumors. The observation of palmar flexion creases may prove to be rewarding in future studies of cellular defense mechanisms in young patients with neoplasia.


Systemic artery-to-pulmonary vein fistula in osteogenic sarcoma of the chest wall. An arteriovenous fistula between the systemic and pulmonary circulations may be congenital, as in bronchopulmonary sequestration, or the communication may be acquired. Inflammatory disease, trauma, and, rarely, neoplasm have been implicated as possible causes of acquired communications. We describe a patient who had a systemic artery-to-pulmonary vein fistula that was secondary to a recurrent sarcoma of the chest wall. Review of the literature failed to reveal a previous report of a similar case.


Bone scanning in management of metastatic osteogenic sarcoma. Bone scan findings are presented from five consecutive cases of metastatic osteogenic sarcoma. Every patient had pulmonary metastases which concentrated the bone imaging radiopharmaceutical to some degree. In one patient, the diagnosis of pulmonary metastasis was made prior to our seeing any radiographic abnormalities. The mechanism by which skeletal imaging agents localize in pulmonary lesions is not clear. Unsuspected skeletal metastases were also discovered on scans in two patients, both of whom had normal radiographs. Diagnosis of hypertrophic pulmonary osteoarthropathy was made in one patient with normal radiographs. These findings indicate that bone scans as well as radiographs should be performed routinely in preoperative staging and followup of patients with osteogenic sarcoma.


Effects of 5-methoxy-N,N-dimethyltryptamine on behavior and raphe unit activity in freely moving cats. 5-methoxy-N,N-dimethyltryptamine (5-MeODMT) produced a dose-dependent decrease in the discharge rate of serotonin-containing neurons in the dorsal raphe nucleus of freely moving cats. This ranged from a 15% decrease at 10 microgran/kg, i.m., to a virtual complete depression of activity at 250 microgram/kg. 5-MeODMT's effects on raphe units occurred with a very short latency (3-5 min) and its duration of action was dose-dependent and limited to an hour or less. The degree of depression of raphe unit activity was directly related to the frequency of occurrence of a number of hallucinogen-specific cat behaviors such as limb flick and abortive groom. There was also a close temporal correlation between the depression of raphe unit activity and the occurrence of these behaviors. These data indicate that the effects of 5-MeODMT may be primarily dependent on its actions upon brain serotonin neurons.


Morphological evidence for the presence of the endogenous guinea-pig retravirus in the lymphoblasts of L2C leukemia. Peripheral blood samples of a transplantable guinea-pig leukemia revealed that, only during the terminal stages of the disease, leukemic cells produce virus particles indistinguishable from the RNA tumor viruses of the Retraviridae family genus Oncorna virus B.


Use of albumin gradients for X and Y sperm separation and clinical experience with male sex preselection. Semen samples obtained from 18 normal males and 37 husbands requesting male child preselection were separated on concentration gradients of human serum albumin. Separated semen obtained from the husbands was then used for artificial homologous insemination (AIH). A significant increase in the sperm motility, progressive drive, and percentage of Y-bearing sperm along with a decrease in the total sperm count and percentage of abnormal forms were observed in separated specimens. Fathers of three or more female children had a slightly smaller but statistically significant percentage of Y-bearing sperm in their semen samples than did normal males. Ten conceptions were achieved with separated semen. Seven pregnancies terminated in normal deliveries of five male and two female infants, one ended in a spontaneous abortion of a male fetus, and two patients are still expecting. The ratio of male to female conceptions in this small study parallels the ratio of Y to X sperm in the final specimen used for AIH.


Relationship between serum copper and bone marrow blasts in acute leukaemia. Investigation of serum copper level (SCL) and bone marrow obtained on the same day from 45 children with acute lymphoblastic leukaemia (ALL) showed a highly significant correlation between SCL and marrow blasts. The increase of SCL was associated with the progress of ALL and the elevation of the marrow parablast percentage. The highest mean SCL level was observed in cases displaying full marrow involvement with concomitant hyperleukocytosis and/or leukaemic tumours. These observations suggest that SCL can be useful in the management of children with acute leukemia.


Critical issues in the JCAH accreditation program for community mental health services. Since its introduction in 1976, the accreditation program for community mental health centers and services has been the focus of considerable comment, both positive and negative. Much of the criticism of the program derives from its theoretical framework, the Balanced Service System, with its unfamiliar service structure and terminology. The author, who played a leading role in developing the accreditation program, discusses the major concerns of the critics and the program revisions that have been made to deal with them while retaining the benefits of the BSS model. He emphasizes the importance of the model in providing a way to uniformly review the widely varying program and administrative configurations that operate as community mental health service programs.


A new chromosome anomaly in acute lymphoblastic leukemia (ALL). A new chromosome anomaly in acute lymphoblastic leukemia (ALL) is reported. Three, possibly four, patients showed an identical karyotype anomaly, characterized by a (4;11)(q13;q22) reciprocal translocation. This anomaly has not so far been found in lymphoproliferative disorders other than ALL. Two of the patients had congenital leukemia, but the anomaly described appears to be more characteristic of ALL than of congenital leukemia, and may help the clinician in establishing the diagnosis of ALL.


Tomography of the lamina dura.

Tomography of the lamina dura. Tomographic technics are based on the principle that when the X-ray source is moved in relation to the object the speed of the projection of different parts of the object on a plane will be different. By selecting the projections which have a certain speed, an image of a selected plane of the object can be obtained. Two principles have to be considered: 1. the image of a curved surface will only show the surface area where the rays form a tangent to the surface; 2. in tomography the blurring of the image increases with an increase of the tomographic angle and the distance of the object to the plane in focus. The image formation of curved bony surfaces, such as the lamina dura, is discussed in detail. The specific properties of the spatial model of the lamina dura requires another approach to explain the image formation. Diagrams and graphs illustrate the effect of the tomographic movement of the X-ray source on the movement of the projected image. Limits for the misalignment permitting an interpretable image are given, as well as an explanation of the images obtained when these limits are exceeded.


Applications of xeroradiography in dentistry-a review. Xeroradiography is the technique in which electrostatically charged plates sensitive to X-rays are used in diagnostic radiology in place of conventional film. There has however been anxiety that radiation dosage for xeroradiography may be at unacceptably high levels. James et al., however, in 1973 showed that by increasing the kilovoltage to at least 120 the exposure could be reduced by 60 per cent. Using higher kV lateral oblique jaw and lateral and anteroposterior skull xeroradiographs have been produced with lower radiation exposure than conventional film. Bony detail is much more sharply delineated on xeroradiographs and soft tissues are visible on the same picture without use of a grid or wedge filter. These features are of obvious advantage in cephalometrics and orthognathic surgery. Panoramic techniques are potentially the most useful way of applying xeroradiography. The combination of full jaw coverage with the sharp definition only possible at present with intra-oral radiographs would provide more information for the dentist, save time for the radiographer and reduce the dose to the patient. Excellent results have been obtained with autopsy specimens on machines which develop 90kVp, but optimal exposure for a normal adult requires a panoramic X-ray machine development 120 kVp. Xeroradiography has the advantage, therefore, of providing more detail of diagnostic value with lower radiation exposure to the patient. The process requires no silver, which is in increasingly short supply.


Inheritance of allozymes of a marine snail (Urosalpinx cinerea). Crosses using virgin oyster drills (Urosalpinx cinerea) show that both the ODH and LAP loci of foot muscle tissue have two co-dominant alleles segregating in Mendelian fashion. This constitutes the first demonstration of allozyme inheritance in a marine gastropod.


Genetics of hydroxyacid oxidase isozymes in the mouse: localisation of Hao-2 on linkage group XVI. Electrophoretic variants of individual isozymes of L-alpha-hydroxyacid oxidase (HAOX-B4) and amylase (AMY-A) in an Asian subspecies of mouse, Mus musculus castaneus, have been used to localise the gene encoding the HAOX B subunit. The structural gene loci for these isozymes (Hao-2 and Amy-1) are apparently linked (4.9 +/- 2.4 per cent recombinants) in this organism, which places Hao-2 on linkage group XVI, since previous studies by Eicher and co-workers (1976) have localised Amy-1 on this chromosome.


Osteosarcoma with small cells simulating Ewing's tumor. Twenty-four patients who had osseous lesions with histological features of both Ewing's sarcoma and osteosarcoma were studied. Because of their appearance, these lesions have been labeled small-cell osteosarcomas. They appear to represent a definite histological entity and the prognosis may be worse for these lesions than for conventional osteosarcomas. It is most important not to misdiagnose this tumor as Ewing's sarcoma because it appears not to be radiosensitive. Radical surgery and modern adjuvant chemotherapy seem to offer the best chance of cure.


Comparison of chloride transport in mouse erythrocytes and Friend virus-transformed erythroleukemic cells. Friend erythroleukemic cells, which grow continuously in tissue culture, resemble in many respects early precursors of mouse erythrocytes. To determine whether or not the membranes of these cells exhibit the rapid and selective exchange of chloride, a specialized feature of the mature erythrocyte membrane, anion fluxes were compared in Friend cells and mouse erythrocytes. The chloride flux in Friend cells at 37 degrees C was about 800-fold lower than in mouse erythrocytes (extrapolated from data at lower temperatures). This difference could not be accounted for by the somewhat lower chloride concentration in Friend cells relative to erythrocytes. Comparison of chloride and sulfate fluxes revealed that the Friend cells had over a 1,000-fold lower selectivity for chloride versus sulphate than did the mouse red cells. The temperature dependence of chloride fluxes in Friend cells corresponded to an Arrhenius activation energy of 17.9 kcal/mol, in contrast to over 30 kcal/mol for mature red cells. The chloride flux in Friend cells was also 10-fold less sensitive to the inhibitor, furosemide, than was the flux in mature red cells. The selective chloride exchange system of the mature erythrocyte therefore does not seem to be functional at the stage represented by the Friend cell, and must appear at some later stage of erythroid maturation.


The effectiveness of dental floss in reducing gingival inflammation. Unwaxed and waxed dental floss were clinically evaluated for effectiveness in reducing gingival inflammation. Two different scoring methods were employed. Both types of floss, when administered by a dental hygienist, were very effective, but no significant differences between them could be found. Both scoring methods led to the same conclusions, but one was more sensitive and provided more detailed information.


The appearances of common dental diseases on radionuclide bone images of the jaws. Radionuclide images of hospital patients were examined for abnormal areas within the jaws. Fourteen of 25 subjects studied showed one or more abnormal image areas, most of which were attributable to common dental lesions revealed by oral examinations. The lesions detectable on images included healing bone sites, periodontal and pulpal disease, residual osteitis and irritations caused by ill-fitting dentures.


Analysis of the Iraqi dentition: mesiodistal crown diameters of permanent teeth. Plaster casts of the permanent dentition of 161 Iraqis were subjected to odontometric analysis. Sex dimorphism was clear in all teeth, the highest being in the canines and lower first molar. Comparisons of the Iraqi dentition with similar population groups were made.


The hydration of dental cements.

The hydration of dental cements. A study was made of the hydration of dental cements, water being classified as "non-evaporable" and "evaporable". The ratio of these two types of water was found to vary greatly among different cement types, being lesser in zinc oxide and ionic polymer cements and greater in ion-leachable glass and phosphoric acid cements. The cement with the least "non-evaporable" water, i.e., showing least hydration (the zinc polycarboxylate cement), had the lowest strength and modulus and the greatest deformation at failure. A linear relationship was found to exist between strength and the degree of hydration of dental cements. All the cements were found to become more highly hydrated and stronger as they aged.


The structure of a glass-ionomer cement and its relationship to the setting process. The G-200 glass of the glass-ionomer cement has two phases: a continuous calcium aluminosilicate matrix and partly crystalline calcium fluoride-rich droplets, the nature of which depend on the thermal history of the glass. The setting process of the cement takes place when the glass is mixed with poly(acrylic acid). It has two overlapping stages corresponding to the rapid leaching of calcium ions from the uncrystalline part of the droplets, followed by the slower release of aluminum (and some calcium) from the main glass phase. These processes are affected by the microstructure and microcomposition of the glass.


A study of high copper amalgams. IV. Formation of eta Cu-Sn (Cu6Sn5) crystals in a high copper dispersant amalgam matrix. In an HCD amalgam, eta Cu-Sn crystals were found dispersed within gamma1 matrix areas. Previously, eta Cu-Sn phase was thought to form only as part of a reaction zone surrounding Ag-Cu dispersant particles. The eta Cu-Sn crystals found in matrix areas of this HCD amalgam are smaller and more widely scattered than eta Cu-Sn crystals dispersed in the gamma1 matrix of HCSC amalgams.


Compressive fatigue limits of composite restorative materials. Compressive fatigue limits at 5,000 stress cycles have been measured for seven commercial composite restorative materials. The average of the ratios between fatigue limit and compressive strengths is 0.64.


Wear of composites by abrasives of varying hardness. The relationship between the wear of three composite resins and the hardness of the abrasive was studied by a two-body abrasion test. The wear rates of the composites increased linearly with abrasive hardness from 530 to 2080 KHN. Measurement of the slope of the wear rate versus abrasive hardness over this range provided a sensitive method for ranking the composites.


Influence of lithium upon dental caries in the rat. Two studies were performed on rats to determine the post-eruptive cariostatic effects of lithium alone and in combination with fluoride. The results failed to demonstrate any cariostatic effects of lithium when evaluated in this manner, nor any enhancement by lithium of the cariostatic activity of fluoride.


Localized fluoride release from fluorine-carrying polyphosphonates. Fluoride release from fluorine-carrying copolymers of vinylphosphonate induced by calcium apatite and tooth enamel has been investigated. Fluoride ions were determined potentiometrically in the study of calcium hydroxyapatite, and Auger spectroscopy was used to study the fluoride release to enamel. The adsorbed copolymer of vinylphosphonic acid and vinylphosphonyl thiofluoride was shown to release fluoride to calcium hydroxyapatite and to enamel. The oxygen analog has shown a similar behavior  with calcium hydroxyapatite, but not with tooth enamel. The results suggest a potential application of such copolymers as caries preventive agents which combine the effects of polyphosphonates and of fluoride ions.


Electrophoretic patterns of proteins from the dentary, vertebra and scale in the ayu fish, Plecoglossus altivelis, Temminck et Schlegel. The electrophoretic patterns of the proteins extracted from dentary, vertebra and scale with the phosphate buffer, 0.1 NHcl and SDS solution were studied to investigate their molecular aspects. The banding patterns and relative amounts of the proteins from dentary, vertebra and scale showed a basic similarity.


The effects of systematic human relations training on freshman dental students. Freshman dental students received training in communication skills via a systematic human relations model. The 35 students participating in the program substantially improved their ability to discern feelings of patients and to communicate with patients in a more understanding and accepting manner. The students thought that the training was beneficial and highly relevant to their future roles as dentists. This kind of training could be offered successfully to other dental health professionals through programs in continuing education.


The caries-preventive effect of amine fluorides and inorganic fluorides in a mouthrinse or dentifrice after 30 months of use. The study groups using a dentifrice and mouthrinse both containing fluorides, a dentifrice containing stannous fluoride and a mouthrinse containing sodium fluoride, or a mouthrinse containing sodium fluoride with a placebo dentifrice had a 20.7% to 29.0% lower DMF increment than the control group after 30 months. These differences were significant. The study groups using a dentifrice containing amine fluorides and a placebo mouthrinse, a mouthrinse containing amine fluorides and a placebo dentifrice, or a dentifrice containing stannous fluoride and a placebo mouthrinse had a 13.6% to 22.4% lower DMF increment than the control group. These differences were not statistically significant. There was no significant difference in effectiveness against caries between the use of the organic or inorganic fluoride products.


Perceptions of dentofacial morphology by laypersons, general dentists, and orthodontists. Line drawings of facial profiles and color photographs of dentitions were evaluated subjectively by 1,150 parents, 72 general practictioners of dentistry, and 54 orthodontists with regard to the normality and abnormality in dentofacial morphology and the need for orthodontic treatment. A significant difference was found between the evaluations of the parents and the professional groups in ten of the 11 facial profiles and in seven of the 11 photographs of dentitions. In general, the parents considered more of the examples acceptable and not requiring orthodontic treatment than did the professional groups. The dentists and orthodontists deviated significantly in their ratings onjy in their judgement of "ugly duckling" example (picture 22, higher abnormal rating by the dentists) and the profile example 10 (higher abnormal rating by orthodontists).  number of orthondontists may have associated the conditions in picture 10 with a Class II, Division 2 malocclusion and, as such, scored it abnormal.


Murocmycotic slough of nasal floor and palate in the anephric patient. A case of nasopalatine mucormycosis in a patient with chronic renal failure is reported. Early recognition is contingent on histopathological examination of tissue and culturing. Vigorous treatment with amphotericin B and surgical intervention averted possible orbital-cranial involvement and effected a cure. When a chronically debilitated patient has signs of intraoral necrosis or facial swelling and necrosis, or both, the clinician should be alert to the possibility of a mucormycotic infection.


Rhinolithiasis: a disorder not to be approached transorally. Rhinolithiasis is a disorder that results from the calcification of intranasal foreign bodies. An unusual case is presented in which a rhinolith was discovered on a maxillary occlusal radiograph. The rhinolith was initially confused with a lesion of the palate. Precise localization of lesions is mandatory before transoral exploration is attempted.


Transoral removal of a fractured odontoid process. The indications for an anterior approach are lesions located predominantly in the body of the vertebra or intervertebral disks. Among these are spondylolisthesis, tuberculosis of the spine, prolapsed intervertebral disks, neoplasms, spinal biopsies, correction of fixed spinal curves, fractures, and fracture dislocations of the spine. Despite many indications, this approach is rarely used, primarily because of unfamiliarity with the surgical field, instrumentation, and oral microbiota. Use of appropriate disciplines and consultations enabled this patient to receive comprehensive treatment from a community of health care specialists.


Human serum hexosaminidase: elevated B form isozyme in cancer patients. The activity of N-acetyl hexosaminidase (beta-2-acetamido-2-deoxy-D-glucoside:N-acetamido-deoxyglucohydrolase, E.C. 3.2.1.30) was significantly greater in sera of patients with solid malignant tumors than in sera of healthy volunteers or of patients with nonmalignant ailments. The increased activity was found in the two major isozymes, hexosaminidase A and hexosaminidase B, but the increase in the latter isozyme was more prominent than in the former. Measurement of the levels of the hexosaminidase isozymes may provide an ancillary diagnostic test for malignancy.


Coenzyme Q deficiency in aged mice.

Coenzyme Q deficiency in aged mice. The specific activities of the succinate dehydrogenase-coenzyme Q reductase were determined in mitochondria from the thymus and the spleen of aged mice (20, 22 and 24 months) as compared with young mice (10 weeks). Significant steep escalation of the deficiency of coenzyme Q-enzyme activity was observed in the thymus of all three groups of aged mice. No significant deficiency was found in the mitochondria of the spleen. The ratios between the liver weight:body weight and the spleen weight:body weight in young and aged mice are practically unchanged, but the thymus weight:body weight ratio decreases significantly in all three groups of aged mice. The described age-dependent anatomical and functional alterations in the thymus most likely form the base for the development of the T cell determined suppression of the immunological responsiveness, present in aged mice.


Dupuytren's disease presenting as palmar pits. A 37 year old man with a non-Hodgkin's lymphoma and a history of heavy alcohol use presented with bilateral palmar pits. Similar lesions were noted in several family members, some of whom had limited use of the hands. Although the patient was referred for evaluation of the palmar pits of the basal cell nevus syndrome, close evaluation revealed the diagnosis of Dupuytren's disease.


Mucosal iron binding proteins in sex-linked anemia and microcytic anemia of the mouse. The duodenal mucosa of genotypically normal iron replete and iron deficient mice and mice with sex-linked (sla) and microcytic anemias (mk) was examined for the presence of iron-binding proteins. Following continuous, 15 or 120 minute, in vivo intraenteric exposure of a closed duodenal loop to 59Fe, a high speed supernatant of homogenized mucosal tissue was chromatographed on G-200 Sephadex. Two major peaks of 59Fe activity were observed. The molecular weight, and immunological properties of peak I were similar to ferritin whilst those of peak II were similar to transferrin. The distribution of 59Fe between peaks I and II in mk/mk animals was similar to that in genotypically normal iron deficient animals indicating that the intramucosal mechanisms for iron transport were reacting appropriately to the iron deficient state of mice with microcytic anemia. In contrast, the distribution of 59Fe between peaks I and II in sla/Y animals was the reverse of that found in genotypically normal iron deficient animals suggesting the possibility of an intramucosal iron binding protein defect in sex-linked anemia.


Treatment of hyperinsulinism after partial pancreatectomy: medical or surgical? A 21 year old male patient with hypoglycemia secondary to hyperinsulinism had no palpable adenoma at surgery, and failed to respond to a partial (75%) pancreatectomy. Subsequently, he was begun on oral Diazoxide. Soon after initiation of Diazoxide he developed a viral pneumonitis, and because of hyperglycemia, the Diazoxide was temporarily discontinued. He has been treated since June of 1975 with Diazoxide, 100 mg, three times daily. He is asymptomatic, completely rehabilitated, and physically active at work for 10 hours daily. Diazoxide therapy may be an option worth considering even in benign states of hyperinsulinism when surgery has failed to correct the process.


Statistical analysis of an urban population of 236 patients with head and neck pain. Part I. Patient profile. A large group of patients (236) was examined for myofascial pain dysfunction syndrome. The parameters used for diagnosis of the syndrome were pain and tenderness in the head and neck muscles and temporomandibular joint, joint sounds, and limited function. It was found that the demographic profile of our patient population did not vary greatly from those reported previously in other studies. However, we did examine more details of the patients' backgrounds than any other single study. The relationships of age, sex, occupation, marital status, emotional stress, and head trauma were discussed.


Statistical analysis of an urban population of 236 patients with head and neck pain. Part II. Patient symptomatology. A large group of patients (236) was examined for Myofascial Pain Dysfunction Syndrome. The symptoms of the patients were recorded, using as a guide the classic parameters of pain and tenderness to the head and neck muscles and temporomandibular joint, joint sounds, and limited function. Our basic data were similar to those of other studies. The significant difference of this study is the detail with which the symptoms were studied. The relationships of suspected etiology and symptoms were compared and discussed. This paper is the second of a three part series. The first part dealt with the demographic profile of our patient population. The third part will present the data dealing with treatment modalities and relief.


Statistical analysis of an urban population of 236 patients with head and neck pain. Part III. Treatment modalities. Data collected from standardized temporomandibular joint examination forms and treatment records of patients diagnosed as having Myofascial Pain Dysfunction Syndrome and treated using occlusal adjustment as the primary mode have been presented. A higher success rate was found in those patients: (1) with a chief complaint of pain confined to the area of one or both temporomandibular joints only, (2) whose answer to location of pain  on initial examination was identified as confined solely to the region of one or both temporomandibular joints, (3) with muscle tenderness to palpation to one or both lateral pterygoid muscles with no other muscle involvement, (4) recorded as having a centric discrepancy in the absence of a balancing side prematurity, and (5) in the 31 to 40-year-old age group.


The prevalence and distribution of gingivitis and gingival recession in children and young adults in Lagos, Nigeria. The prevalence and distribution of gingivitis and gingival recession were studied in 820 Nigerians aged 15, 19, 20 and 21 years sampled from Lagos educational establishments. The prevalence of gingivitis was relatively high at all ages. There was a high degree of correlation between the prevalence of gingivitis affecting mouths, papillae and margins. It therefore would appear that in those mouths with gingivitis, the proportion of affected papillae or margins to those not affected varies only within narrow limits. Although the peak prevalance of gingivitis was observed in the 15-year olds, the prevalence of gingival recession was higher in the 21-year-old students than in those aged 15 years. Gingival recession may be a sequel of gingivitis resulting from apical proliferation of the epithelial attachment with destruction of the subjacent periodontal tissues. Hence gingivitis should be distinguished from gingival recession in its prevalence and distribution.


Oral discoid lupus erythematosus. A case report and review of the literature. Literature on systemic and chronic discoid lupus erythematosus has been reviewed. The clinical and histopathologic changes in the oral cavity produced by these disorders are emphasized. A case of chronic lupus erythematosus with oral lesions is reported.


The influence of heat treatments on several types of base-metal removable partial denture alloys. Four removable partial denture alloys, Vitallium (Co-Cr alloy), Dentillium P.D. (Fe-Cr alloy), Durallium L.G. (Co-Cr-Ni alloy), and Ticonium 100 (Ni-Cr alloy), were evaluated in the as-cast condition and after heat treatment for 15 minutes at 1,300 degrees, 1,600 degrees, 1,900 degrees, and 2,200 degrees F followed by quenching in water. The following properties were determined and compared for each alloy at each heat treatment condition: the yield strengths at 0.01%, 0.1%, and 0.2% offsets, the ultimate tensile strength, the percent elongation, the modulus of elasticity, and the Knoop microhardness. The results were statistically analyzed. Photomicrographs were examined for each alloy and test condition. The following conclusions were made: 1. The "highest values" were exhibited by the as-cast alloy. 2. Heat treatment of the partial denture alloys tested resulted in reductions in strength, while the elongations varied. This study demonstrates that, in practice, one should avoid (a) prolonged "heat-soaking" while soldering and (b) grinding or polishing of the casting until the alloy is "red hot". 3. Durallium L.G. was the least affected by the various heat treatment conditions. 4. Conventional reporting of the yield strength at 0.2% offset, the ultimate tensile strength, and percent elongation are not adequate to completely describe and compare the mechanical behavior of alloys. The reporting of the yield strength at 0.01% offset, in addition to the other reported properties, will provide a more complete description of the behavior of the dental alloys.


Custom-made perforated cyst decompression stents. A method of facilitating cyst decompression has been presented. By using available materials and equipment, perforated silicone stents can be made to specific dimensions in a relatively short time period for this purpose.


Use of silicone in treatment phase of radiotherapy. The usefulness of silicone has been demonstrated. No special materials are necessary, as those which are already present in the dental office are adequate. The silicone will not distort over the treatment period and can be repositioned accurately to ensure uniformity of the dose. It is also easily cleaned and stored for daily use. It can be used to improve the plane of the surface tissue and provide "buildup" to fill "dead space," which helps assure accurate assessment of the magnitude and distribution of the desired radiation dose.


A clinical study of rest position using the Kinesiograph and Myomonitor. The clinical study was designed to study the vertical dimension of rest position and mandibular closure to maximum intercuspation of the teeth before, during, and after relaxation procedures on 14 subjects with stable dentitions. The findings call attention to the potential of both the Myomonitor and the Kinesiograph for research and clinical use, to the physiologic need for flexibility of the vertical dimension of rest position, and to the importance of measuring all three dimensions when examining the rest position.


Computer-monitored radionuclide tracking of three-dimensional mandibular movements. Part II: experimental setup and preliminary results--Posselt diagram. This article described a new method to track mandibular movements using a computer-assisted radionuclide kinematics technique. The usefulness of various image-enhancement techniques is discussed, and the reproduction of physiologic displacements is shown. Vertical, lateral, and protrusive envelopes of motion of a point on a tooth of a complete denture mounted on a semiadjustable articulator were measured. A demonstrative example of the validity of this approach is reproducing the motion of the mental point, which clearly evidences the Posselt diagram.


Aspects of planning and evaluation of children's dental care in Appalachia. The dental public health literature contains almost no information on caries experience and treatment needs of Appalachian children, and little information concerning resource requirements for children's dental care programs in general. Data from three dental treatment programs in Appalachia are summarized for purposes of planning and evaluation of similar programs. Age specific caries and service experiences are presented, and evaluation criteria and planning considerations are discussed. Resource requirements, including time, cost and manpower are also presented, and special considerations for planning programs are discussed.


The HSA: a focus for advancing primary preventive dental programs. In conclusion, as community agencies under P.L. 93-641 embark upon the definition of health status goals, health system goals, and program implementation, dentists have the opportunity to clarify and assist in developing a vigorous approach to meeting dental health needs through primary prevention. Insofar as public health dentistry is able to provide technical assistance and leadership at the local HSA and SHPDA levels, the powerful tools for change embodied in this law can be a force for promoting the oral health of our population.


Osteosarcoma in a patient with Hutchinson-Gilford progeria. A 13-year-old female with Hutchinson-Gilford progeria, who developed an osteosarcoma of the right chest wall, is reported. This is the first reported association of a malignant neoplasm with this syndrome.


Lipoidal bladder concretion: an unusual autopsy finding in acute lymphoblastic leukemia. The occurrence of a large (2.3 gm.), wax-like, creamy white concretion in the bladder of a 7-year-old girl who died of acute lymphoblastic leukemia is reported. Lipids constituted 40% of the total weight and triglycerides accounted for 95% of the total lipids. The fatty acid composition of the individual lipid classes showed a predominance of saturated fatty acids (75 to 80%). Factors influencing the pathogenesis of the material are discussed. A review of the literature on childhood leukemia showed no reference to such lipid abnormalities and the occurrence of this type of concretion has not been reported previously.


Bone marrow polyamines in children with acute leukemia as related to remission status, therapy, and cellularity of specimens. Measurements of polyamines have potential practicality as biochemical markers of disease activity in adult cancer patients. On this basis, similar measurements were made in children with leukemia and solid tumors. Fluctuations in blood polyamine concentration were studied in relationship to chemotherapy schematics, cellularity and composition of blood and bone marrow specimens, and remission status of patients. Our results indicate that each of these factors may influence these determinations and consequently their clinical usefulness.


Dissimilar courses of twins with leukemia. Twin girls, genetically identical, probably experienced different leukemogenic events and presented with acute lymphocytic leukemia 6 years apart. Their clinical presentations were similar, but they received significantly different therapy. The first twin died 34 months after diagnosis following multiple remissions and relapses, having received single-drug maintenance. The second twin remains free of apparent disease 60 months after diagnosis, following vincristine and prednisone induction, 6-mercaptopurine maintenance, methotrexate and prednisone reinforcement, and central nervous system treatment of occult disease. Their dissimilar clinical courses may have been due to different leukemogenic events and/or markedly different therapeutic programs.


Adriamycin in combination chemotherapy of adult acute lymphoblastic leukemia: a Southwest Oncology Group study. Eighteen (72%) of 25 evaluable and previously untreated patients with adult acute lymphoblastic leukemia entered complete remission (CR) following induction therapy with adriamycin, vincristine, and prednisone in a Southwest Oncology Group study. Remission maintenance therapy with methotrexate and 6-mercaptopurine resulted in a median duration of CR of 10.2 months. The addition of Adriamycin to prednisone and vincristine may be beneficial in slow responders or nonresponders to these two drugs and in patients with initially high peripheral blood blast counts.


Clinical and cytokinetic aspects of remission induction of childhood acute lymphoblastic leukemia (ALL): addition of an anthracycline to vincristine and prednisone. Fifty-six untreated patients with childhood with acute lymphoblastic leukemia (ALL) were randomized to receive one of three remission induction regimens: vincristine and prednisone (VP), vincristine, prednisone and daunorubicin (VPD), or vincristine, prednisone and adriamycin (VPA). The complete remission rate was similar for all three groups. Although the anthracycline regimens caused somewhat more rapid leukemic cell reduction than the VP only group, this difference was not significant. Labeling index reduction between study days 1 and 5 was significantly greater (p less than 0.001) with an anthracycline than for the VP group, but there was no difference between the two anthracyclines. Granulocytopenia during induction was significantly increased (p less than 0.05) in both the VPD and VPA groups as compared with VP alone. A significantly higher rate of infectious morbidity (p less than 0.01) was associated with the addition of either anthracycline, but to date no significant differences in remission duration or survival have been observed. The addition of anthracyclines to VP for remission induction in childhood ALL has theoretical advantages, but may be undesirable because of increased morbidity.


Characteristics of long-term survivors in AML. We examined the records of all patients with a diagnosis of acute myelogenous leukemia or acute myelo-monocytic leukemia seen between 1963--1975. Of 211 patients, 12 are alive and in complete remission from 179--48 months since diagnosis. Life table analysis reveals a survival plateau of 4--5% after five years. Three of the survivors have suffered relapses but still survive without evidence of disease. The clinical and laboratory characteristics of all the survivors are presented. The survivors have a significantly younger age at diagnosis than the nonsurvivors (P less than 0.005). However, no other factors could be identified as characteristic of the survivors' group.


The lack of correlation of gallium (67Ga) scans with known prognostic indicators in childhood acute lymphocytic leukemia. 67Gallium-citrate scans were performed on 45 patients with acute lymphoblastic leukemia (ALL) at the time of diagnosis. The extent of uptake and distribution of gallium were compared to known prognostic indicators of age, initial white count, and bone marrow lymphoblast immune markers (T, B, and null cell). Selective increased uptake was noted in bone, kidney, liver/spleen, lymph nodes, or other sites in 29 patients, and 16 patients had "normal" scans. A scoring system giving one point for selective uptake in each organ system was used. Patients were grouped according to scores of zero, 1--2, and 3 or greater. There was no significant correlation found between total gallium scores and any of the three prognostic indicators. There was also no significant correlation when prognostic factors were compared to uptake in the individual organ systems except that T cell disease was associated with a significantly greater propensity for lymph node uptake. There was a suggestion that children with "normal" gallium scans may have a better long-term prognosis, since 6 of 7 nonrelapsers observed for longer than 18 months had scores of zero at diagnosis compared to 4 of 6 early deaths with scores greater than zero. This study indicates that the 67Ga scan does not significantly correlate with known prognostic indicators, with the possible exception of lymph node uptake in T cell disease, and its value as an independent indicator of long-term survival will require longer follow-up.


Ph1-positive CML in a 13;14 translocation carrier. A 63-year-old woman with Ph1-positive CML and a constitutional 13;14 Robertsonian translocation is described. The rarity of this cytogenetic combination and the significance of the D/D translocation in the genesis of the Ph1 and other chromosome anomalies in myeloproliferative disorders is briefly discussed. It is concluded that the occurrence of the two cytogenetic anomalies and the clinical states is coincidental.


Accidental intrathecal administration of vincristine. Vincristine is a chemotherapeutic agent used in the treatment of various neoplastic diseases. Its neurotoxicity after therapeutic and pharmacologic doses has been well documented. We report a case of accidental intrathecal administration of vincristine in order to describe the complications seen, discuss possible means of therapeutic intervention, and serve as a reminder that preventive measures are mandatory to avoid such accidents in the future.


[Free gingival graft in mucogingival surgery]. Palatine grafts, which today represent the simplest and most reliable technique for enhancing the adherent gum--a problem of great importance in periodontal treatment--are discussed together with other techniques designed for the same purpose. Bone site palatine grafts which restore adherent gum on the alveolar bone, are distinguished from osteo-dental site grafts which are proposed for the treatment of split gums and which only take partially on the radicular surface.


[Cancer of the tongue. Anatomopathological and clinical considerations]. Reference is made to the percent incidence of malignant tumours of the tongue and its increase. Several anatomical features of this form are described, with particular regard to the lymphatic pathways. Turning to questions of classification, attention is given to cancer of the soft and fixed parts of the tongue in the light of the clinical stages and TNM system (1973) with the aim of making statistical comparisons more uniform, especially those concerning the approach adopted and the results achieved by modern, interdisciplinary therapy.


[Synthetic materials in reconstructive maxillofacial surgery]. Surgical or traumatic losses of substances should preferably be made good by autografts, though "alloplastic implants" are a sound alternative. An account is given of the chemical and physical properties of intrinsic and manmade synthetic polymers. The best polymers for the preparation of endoprostheses are indicated, and reference is made to their more common indications in maxillofacial reconstructive surgery.


[Lateral sliding flaps in mucogingival surgery]. Reference is made to parodontal surgery techniques employing lateral sliding flaps mucogingival for the treatment of gingival schisis. Grupe & Warren (1965) proposed the use of a flap from the parts contiguous to that affected by the schisis. The technique was then developed by the introduction of bipapillar flaps and a flap taken from a toothless saddle. All operations using sliding flaps form part of the most difficult area in mucogingival surgery, due to difficulty of securing the "taking" of the flap on the root cement of the parts affected by schisis.


[Computerized axial tomography in dentistry. Preliminary note]. Early results in the use of computerized axial tomography (C.A.T.) in odontostomatology are reported. The results obtained with this new technique open new horizons in the clinico-radiological diagnosis of maxillofacial conditions.


[A case of osteochondroma of the mandibular condyle]. A case of mandibular condyle osteochondroma is reported, special attention being paid to radiographic aspects and to the functional changes brought on by this neoplasia. Surgical and meta-operative treatment to correct residual latero-deviation and occlusal relations are described.


Cranial computed tomography in leukaemia. The computed tomograms performed on a series of 41 patients during the course of prolonged treatment for acute leukaemia were reviewed. Thirty-one of these cases were referred for some neurological disturbance, the remainder for routine assessment. Abnormalities were present in 59%. In two cases there were leukaemic deposits, four were due to infection and four to necrotising leucoencephalopathy. Diffuse cerebral swelling occurred in three patients, and ventricular dilatation in 15, associated with enlarged cortical sulci in 13. The relationship of these findings to drug therapy is discussed.


Gilles de la Tourette syndrome after long-term chlorpromazine therapy. Following 6 years of continuous chlorpromazine therapy for schizophrenia, a young woman developed multifocal tics and vocalizations characteristic of Tourette syndrome. The symptoms first appeared when chlorpromazine was withdrawn. They were permanent, although partially ameliorated by chronic haloperidol therapy. Because of her age and past history, these symptoms were attributed to chronic neuroleptic therapy analogous to neuroleptic-induced tardive dyskinesia, rather than to Tourette syndrome per se. These symptoms suggest that chronic receptor-site blockade can result in hypersensitivity of dopamine receptor sites, and that this may play a role in the pathophysiology of Gilles de la Tourette syndrome. This is the first evidence that hypersensitivity of dopamine receptors is involved in the pathophysiology of Tourette syndrome.


Hypoxanthine guanine phosphoribosyltransferase (HGPRT) in Gilles de la Tourette syndrome. Hypoxanthine guanine phosphoribosyltransferase (HGPRT) and adenosine phosphoribosyltransferase (APRT) were examined from 11 individuals with Gilles de la Tourette syndrome, 10 of their first- or second-degree relatives, and 3 normal controls. It has been suggested that in some self-mutilating Tourette patients, HGPRT shows a time-related loss of activity at 4 degrees C, and an unusual isoelectrofocusing pattern. Although 3 patients experienced self-mutilation, no consistent abnormalities were found in the temperature-stability of their HGPRT at 4 degrees C and 70 degrees C, or in isoelectrofocusing of HGPRT purified by immunoprecipitation. An alteration of the purine metabolic pathway in Tourette syndrome has not been established.


The Milton Survey. 2. Blood pressure and heart rate. Blood pressure and heart rate were measured in 1,206 adult inhabitants of Milton, about 82 percent of the adult population. Mean blood pressure for the various 10 year age groups was very similar to that found in other comparable societies. There was quite a high prevalence of blood pressure levels of 160/95 mmHg or over, especially in the older age groups. Blood pressure was lower in young women than in young men but rose more steeply with age in women than in men, so that in old age it was higher in women than men even though antihypertensive treatment was much more common in the women. Heart rate was higher in women than in men; in both sexes, heart rate correlated strongly with blood pressure. Quetelet's index (weight/height) also correlated strongly with blood pressure; this suggests once again that the amount of hypertension in the community might be reduced if body weight could be kept at desirable levels.


Intentional paraquat poisoning: case report. A case of intentional paraquat poisoning is presented. Early treatment was instituted. At four-month follow-up, the patient was well.


Testicular infarction with incarcerated inguinal hernia in infants. Three male babies presenting with incarcerated inguinal hernias in the first year of life were found to have infarction of the ipselateral testis at operation. This is a well recognised complication of indirect inguinal hernia and a common complication of incarceration.


Immunological properties of lymphoblasts and lymphocytes in children with acute lymphatic or undifferentiated leukemia. In 35 children with acute lymphatic or undifferentiated leukemia, immunological properties of normal lymphocytes and lymphoblasts were investigated. In most cases the number of circulating normal lymphocytes with surface or B markers and delayed type hypersensitivity was depressed. On the other hand, the response to mitogenic stimulation with PHA as well as the antibody and immunoglobulin serum concentrations were found to be normal. Lymphoblasts did not exhibit surface receptors in any but four patients, who showed 10--18% of leukemic blasts being positive for E-rosette formation at 0 degree but not at 37 degrees.


Intraoral second division nerve block.

Intraoral second division nerve block. The second division of the trigeminal nerve can be easily blocked by the intraoral route. The technique presented involves entering the greater palatine foramen, traversing the pterygopalatine canal with a needle, and depositing the local anesthetic solution into the superior aspect of the pterygopalatine fossa, where the trunk of the second division lies after the nerve exits the foramen rotundum. The indications, contraindications, technique, and anatomic considerations are presented. This is followed by a discussion of the possible complications as well as their prevention and remedy should they occur. While this is not a new technique, it as been a forgotten one.


Cryosurgical and thermal monitoring system for management of malignancy. A comprehensive evaluation of the major aspects of cryosurgery has been explored in this preliminary study. A thermal monitoring system has been used concomitantly with cryosurgery, and characteristic freezing pattern differences between normal and malignant tissue have been demonstrated. Morphologic examination of the sections from the control and experimental animals subjected to cryosurgery has been valuable to demonstrate consistent tissue damage. This study has also shown the feasibility of implementing a thermodynamic mathematical model to establish the basis for clinical application of cryosurgery on a more rational therapeutic basis.


Temporomandibular joint synovitis with effusion in familial Mediterranean fever. A unique case of TMJ synovitis with effusion associated with FMF is described. This effusion was treated by aspiration, once the diagnosis was established. It is believed that aspiration is the most effective way of avoiding aseptic necrosis and should be carried out as soon as the diagnosis is established. The posterior portion of the compartment is easier to find with the aspirating needle. The use of a corticosteroid in conjunction with aspiration is of questionable benefit in FMF but may play a more important part in effusion from other causes.


Unilateral fracture of the pterygoid process. Report of a case. An unusual case of an isolated unilateral fracture of the pterygoid process is presented. The symptoms include a primary malocclusion as well as paresthesia of the major part of the maxillary nerve, indicating maxillary and/or mandibular fracture with dislocation of the fragments. The symptoms related to the regional anatomy as well as the mechanism of origin of the fracture are discussed.


Reduction of mouth opening due to mandibular coronoid progess enlargement. Report of a case. A case of unilateral mandibular coronoid enlargement associated with facial asymmetry and severe limitation of mandibular movement in a 19-year-old male patient is described. The indications for treatment and the types of surgical approach for removal of the enlarged process are discussed. The dual surgical approach required for resection of the coronoid in this case is described.


Amelogenesis imperfecta among Israeli Jews and the description of a new type of local hypoplastic autosomal recessive amelogenesis imperfecta. Amelogenesis imperfecta (AI) was detected in nine of 70,359 school children surveyed, a prevalence approximating 1:8,000. Of these cases, eight were the hypoplastic type and one the snow-capped hypomaturation type. Family studies demonstrated that hypoplastic AI was an autosomal dominant trait in two children and an autosomal recessive in six. Of three additional families referred to our clinic, two had autosomal recessive hypoplastic AI and one the hypocalcified type, inherited as an autosomal dominant trait. In four families, a new type of local hypoplastic autosomal recessive AI was observed, characterized by horizontal pitting and grooving more pronounced in the middle third of the crowns of most teeth in both dentitions.


Blastomycosis with oral lesions. Report of two cases. The recent literature suggests that blastomycosis (previously known as North American blastomycosis) with oral lesions may be more common than is generally realized. This report discusses two cases of blastomycosis in which oral lesions played a critical role in the ultimate diagnosis of the disease. The potential importance or oral lesions in early diagnosis and the necessity of culturing the organisms for definitive diagnosis are emphasized.


Necrotizing sialometaplasia affecting the minor labial glands. A case of necrotizing sialometaplasia of the lower lip, a previously unreported location for this lesion, is presented. In the past, other cases of this benign process occuring in this site may have been confused with squamous-cell or mucoepidermoid carcinoma. In order to arrive at the correct diagnosis, an ample biopsy of the lesion is mandatory.


Fibrosarcoma of maxilla. Report of a case with histochemical studies. A case of fibrosarcoma of the maxilla with strong alkaline phosphatase activity in the tumor cells is described, and the significance of this enzyme is discussed.


Necrotizing sialometaplasia.

Necrotizing sialometaplasia. An example of palatal necrotizing sialometaplasia is presented, and the clinical and microscopic features suggestive of squamous-cell carcinoma and mucoepidermal carcinoma are noted. The patient's health status, as shown by a rather thorough work-up, was unremarkable during the period which would seem to preclude a systemic etiology of this disease. The resolution of the lesion with minimal therapy is documented.


The prognosis of traumatized permanent anterior teeth with fracture of the enamel and dentin. The purpose of this study was to examine, in the light of a 2-year follow-up, the diagnostic and prognostic value of vitality tests in teeth which had suffered fracture of the enamel and dentin without pulp exposure, to assess the period in which late pathologic changes may occur, and to determine the recommended time intervals for follow-up examinations. Eighty-four childred, 6 to 14 years of age, with 123 traumatized teeth were included in the study. Of these teeth, 87 percent were "vital" at the initial examination, and most of these remained vital throughout the 2 years. Most pathologic changes occur within 6 months after trauma. It is recommended that follow-up examinations be performed 3 and 6 months after the trauma. Root canal treatment is usually necessary in teeth which have no vital response by the 3- and 6-month examinations.


Root canal treatment with calcium hydroxide. III. Effect of debris and pressure filling. Root canals of dog premolar teeth with or without debris were filled with calcium hydroxide with or without pressure. The histologic results, 90 days after treatment, showed better results in the specimens without debris. More favorable results were obtained with the pressure filling when debris was present in small amounts.


Space age cassettes can lower radiation dose requirements. In radiation health principles it is important to increase information per unit of radiation or to decrease radiation per unit of information. This study shows that the use of a new materal for cassette faceplates can provide the radiologist with identical radiographs while exposing the patient to significantly less radiation. The amount of reduction will depend upon the KVP used and the thickness of the material between the patient and the film. It should be possible to reduce radiation up to 90 percent in some x-ray procedures with this new sophisticated Space Age material.


The length of teeth. A statistical analysis of the differences in length of human teeth for radiologic purposes. Intraoral radiograms can be made according to the long tube paralleling technique utilizing aiming devices. An important factor in the design of these instruments is the length of the teeth to be radiographed. Reliable data regarding the length of the teeth in the different regions of the mouth are not available. Information on the length of teeth was therefore collected by measuring 1,400 extracted teeth. A statistical analysis of the data finally resulted for each region of the mouth and for both sexes in length value which could be used for the design of aiming devices.


[Disease and dental health. Opinions of institutional dental care in a group of health personnel]. It is reasonable to expect that dental care for institutionalized persons in Norway will be considerably improved in the near future. Opinions and attitudes towards dentistry of the health personel working in the institutions, will, therefore, be of great interest. The present study which was performed by self-administered questionnaires, showed that most of the health personel at Lier sykehus, a mental hospital near Oslo, were willing to and interested in doing active preventive work. There was, however, lack of knowledge about how to perform preventive dentistry. In the future, information and instruction concerning how to prevent oral disease, will be a meaningful task for the dental health team.


In vitro DNA dependent synthesis of globin RNA sequences from erythroleukemic cell chromatin. Murine erythroleukemic cells in culture accumulate cytoplasmic globin mRNA during differentiation induced by dimethyl sulfoxide (DMSO)1. Chromatin was prepared from DMSO induced erythroleukemic cells that were transcribing globin RNA in order to determine whether in vitro synthesis of globin RNA sequences was possible from chromatin. RNA was synthesized in vitro using 5-mercuriuridine triphosphate and exogenous Escheria coli RNA polymerase. Newly synthesized mercurated RNA was purified from endogenous chromatin associated RNA by affinity chromatography on a sepharose sulfhydryl column, and the globin RNA sequence content of the mercurated RNA was assayed by hybridization to cDNA globin. The synthesis of globin RNA sequences was shown to occur and to be sensitive to actinomycin and rifampicin and insensitive to alpha-amanitin. In contrast, synthesis of globin RNA sequence synthesis was not detected in significant amounts from chromatin prepared from uninduced erythroleukemic cells, nor from uninduced cell chromatin to which globin RNA was added prior to transcription. Isolated RNA:cDNA globin hybrids were shown to contain mercurated RNA by affinity chromatography. These results indicated that synthesis of globin RNA sequences from chromatin can be performed by E. coli RNA polymerase.


DNA sequencing and melting curve.

DNA sequencing and melting curve. The dependence of DNA absorbance (for light at about 260 nm) on temperature is related to a specific DNA sequence structure in the vicinity of DNA thermal denaturation (the so-called DNA melting or coiling). A straightforward analysis of the experimental DNA melting curve allows us to determine the lengths, the A+T content, and the location in DNA of certain domains. In the case of a specific DNA fragmentation, the order of fragments in DNA can be learned from this analysis, nondestructively and quickly, without fractionating the fragments and other methods of fragmentation. If the DNA nucleotide sequence is known except for some sites and uncertain portions, the analysis determines these sites and the accuracy of the sequence at the portions. This information may complement exact methods of DNA sequencing. The proposed analysis is applied to bacteriophage phiX174, whose melting curve is known. The results are compared to and found to be in an excellent agreement with the known phiX174 nucleotide sequence.


Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems. When incubated with a soluble extract from large oocytes of Xenopus laevis, recombinant DNA plasmids containing either X. laevis oocyte 5S DNA or X. borealis oocyte 5S DNA direct the synthesis of discrete 5S RNAs, which by size and sequence analysis are similar or identical to the corresponding 5S RNAs synthesized in vivo. Synthesis of the 5S RNAs is mediated by a soluble endogenous RNA polymerase III (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6), which presumably recognizes specific initiation and termination sites in the 5S genes. Optimal conditions for accurate synthesis and the kinetics of the reactions have been determined. A soluble postchromatin supernatant fraction has also been isolated from immature oocytes. Although devoid of a functional endogenous RNA polymerase III, this extract contains a component(s) that effects the accurate transcription of 5S genes (in a plasmid) by a purified RNA polymerase III.


Lateral diffusion in binary mixtures of cholesterol and phosphatidylcholines. The lateral diffusion of a fluorescent-labeled phospholipid, phosphatidyl-N-(4-nitrobenzo-2-oxa-1,3,-diazole)ethanolamine, has been measured in binary mixtures of cholesterol and dimyristoyl phosphatidylcholine at temperatures both above and below 23.8 degrees C, the chain-melting transition temperature of this phosphatidylcholine. There is a temperature-composition region, approximately temperature less than 23 degrees C and mole fraction of cholesterol (X) less than 0.20, in which the lateral diffusion coefficient of the fluorescent probe is at least an order of magnitude smaller than it is at points outside of this temperature-composition region. At temperatures above approximately 23 degrees C there is a significant increasing cholesterol concentration, for X greater than 0.2.


Location of the regulatory site for establishment of repression by bacteriophage lambda. During the lysogenic response to infection by bacteriophage lambda, the phage-specified cII and cIII proteins provide for the coordinate establishment of repression and integration of the viral DNA. One critical regulatory function of cII/cIII is an activation of synthesis of the cI protein, the repressor that maintains lysogeny. The mechanism and site for regulation of the cI gene by cII/cIII have been a subject of controversy. The two principal hypotheses for cII/cIII action are: initiation of new RNA chains in the y region of lambda DNA just to the left of the cII gene; or antitermination of a short leader RNA (4S or oop RNA) initiated to the right of the cII gene. In an effort to distinguish between these hypotheses, we have studied the cII-mediated turn-on of cI protein synthesis in three classes of prophage deletion strains: deletions of the 4S RNA promoter but not the y region, deletions that remove both regions, and deletions that leave both intact. We find that an intact y region is required for normal regulation of the cI gene by cII, but the 4S RNA promoter is not. From experiments with other mutants, we conclude that rightward transcription from the early lytic promoter is also not necessary for positive regulation. Our results suggest that positive regulation by cII/cIII involves initiation of new RNA chains through activation of promoter sites.


RNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor. We have analyzed the RNA produced in vitro by incubating nuclei from HeLa cells infected with adenovirus serotype 2. Our results show that adenovirus-specific RNA is produced at a linear rate for up to 2.5 hr. Hybridization analysis of RNA produced in nuclei isolated 18 hr after infection indicated that transcription begins at the "late promoter" at map position 16.5. Sequence analysis of the 5' termini of the in vitro transcripts showed that this system initiates RNA chains de novo at the correct promoter and that the 5' terminus is capped.


Caulobacter crescentus nucleoid: analysis of sedimentation behavior and protein composition during the cell cycle. The envelope-associated nucleoid (EAN) of Caulobacter crescentus has been isolated during major developmental stages in the cell cycle. The sedimentation coefficient of the Caulobacter EAN changes as a function of development and is closely correlated with the periodicity of DNA synthesis in this bacterium. The contribution of proteins to the structure of the Caulobacter nucleoid has been analyzed by using polyacrylamide gel electrophoresis of proteins labeled both for short intervals and continuously throughout the cell cycle. The EAN proteins are derived primarily from membranes and are sequentially associated with the EAN during the cell cycle. Several distinct proteins are isolated with the EAN at specific stages of differentiation. A 26,000 Mr protein, which appears uniquely associated with the swarmer cell EANs, was identified and shown to segregate preferentially with the swarmer cell EAN at cell division.


Sterigmatocystin-DNA interactions: identification of a major adduct formed after metabolic activation in vitro. Sterigmatocystin (ST), a potent hepatocarcinogen, was covalently bound to calf thymus DNA by incubation in the presence of phenobarbital-induced rat liver microsomes. Acid hydrolysis of ST-modified DNA liberated a major guanine-containing adduct, present in DNA at an estimated level of 1 ST residue per 100-150 nucleotides. The adduct was isolated by high-pressure liquid chromatography and subjected to structural analysis. Spectral and chemical data identified the adduct as 1,2-dihydro-2-(N(7)-guanyl)-1-hydroxysterigmatocystin, the guanine and hydroxyl moieties being in a trans configuration. The structure and stereochemistry of this adduct indicated that the exo-ST-1,2-oxide was the metabolite that reacted with DNA, and the quantitative yield of adduct indicated that this metabolite was a major product of the in vitro metabolism of ST.


Conformations of twisted parallel beta-sheets and the origin of chirality in protein structures. An analysis of the conformational properties of parallel beta-pleated sheets suggests that an important factor in the generation of beta-sheet twist is the preference for nonplanar peptide bond distortions that impart local left-handed helical character to polypeptide chains. It is demonstrated that the introduction of such chiral distortions, which result from the tetrahedral deformation of the peptide nitrogen atoms, naturally produces right-twisted beta-sheet structures with optimal hydrogen bond geometry.


Helical repeat of DNA in solution.

Helical repeat of DNA in solution. The helical repeat of DNA in solution has been measured directly by analyzing the gel electrophoretic patterns of pairs of covalently closed DNAs with length differences between 1 and 58 base pairs, out of a total length of about 4350 base pairs per DNA molecule. The method is based on the observation that for a covalently closed DNA of a fixed size of n base pairs (n of the order of several thousand), under appropriate conditions, two topological isomers (topoisomers) differing by 1 in their linking numbers are well resolved by gel electrophoresis. If the size of the DNA is increased to n + x base pairs, unless x is an integral multiple of the helical repeat h, the bands of the topoisomers with n + x base pairs per molecule are all shifted relative to the bands of the topoisomers with n base pairs per molecule. The magnitude of the shift is directly related to the nonintegral residual of x/n. Analysis of the set with x ranging from 1 to 58 gives the DNA helix repeat in solution as 10.4 base pairs per turn under physiological conditions, with an estimated probable error of +/- 0.1. This result strongly supports the double helix structure of DNA and rejects the side-by-side model of Rodley et al. [Rodley, G.A., Scobie, R.S., Bates, R.H. T & Lewitt, R.M. (1976) Proc. Natl. Acad. Sci. USA 73, 2959-2963]. The helical repeat of DNA measured in solution is significantly different from the value 10.0 base pairs per turn for the B form fiber structure.


Resolution of adenylate cyclase sensitive and insensitive to Ca2+ and calcium-dependent regulatory protein (CDR) by CDR-sepharose affinity chromatography. Partially purified adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] from bovine brain cortex was fractionated into two separate forms by calcium-dependent regulatory protein (CDR)-Sepharose affinity chromatography. The major form of the enzyme, comprising approximately 80% of the applied activity, did not bind to the affinity column in the presence of Ca2+ and was insensitive to the CDR. Approximately 20% of adenylate cyclase activity was absorbed to CDR-Sepharose in the presence of Ca2+. This activity was stimulated by Ca2+ and CDR. This study directly demonstrates that brain cortex contains Ca2+-CDR-sensitive and -insensitive forms of adenylate cyclase and indicates that CDR-Sepharose may be a useful tool for purification of adenylate cyclase. The Ca2+ -stimulated adenylate cyclase was purified at least 55-fold with a 13% yield.


Biosynthesis and self-assembly of protein S, a development-specific protein of Myxococcus xanthus. Myxococcus xanthus is a Gram-negative bacterium that has a complex life cycle including a temporal sequence of cellular aggregation, mound formation, and myxosporulation. During development, protein S (molecuar weight 23,000) is induced and accumulates in very large amounts. Protein S was found in the soluble fraction of early developmental extracts and in the insoluble fraction in later extracts. This insoluble form of protein S can be solubilized by the addition of 1 M NaCl at 0 degrees C to extracts from aggregated cells (mound stage) or by the addition of 1 M NaCl at 30 degrees C to mature spores. Salt extraction (1 M NaCl) of protein S from mature spores was partially inhibited by the addition of Mg(2+) and almost completely inhibited by the addition of Ca(2+). The viability of spores was not changed by a salt extraction that removed their protein S. Examination of thin sections of mature spores and extracted spores by electron microscopy suggested that the protein S-deficient spores lacked a spore surface coat about 300 A thick. Purified protein S will spontaneously self-assemble onto protein S-deficient spores after removal of the NaCl by dialysis or by addition of 10 mM Ca(2+) to undialyzed samples. Glycerol-induced spores did not contain protein S and did not serve as primers for assembly of protein S. Quantitation of the self-assembly process showed almost stoichiometric binding of protein S to the protein S-deficient spores until saturation at 3.3 x 10(6) molecules per spore, a value 1.35 times higher than the normal level of proteins S found in mature spores. Protein S in the "reconstituted" spores was as protease resistant and sonication resistant as the protein S of native spores. Electron microscopy of the reconstituted spores revealed the assembly of new material on the spore surface. Adjacent spores were sometimes observed to be fused to each other through a common protein S layer. These results suggest that protein S serves a function in spore-spore interaction in the fruiting body.


Partial primary structure of bacteriorhodopsin: sequencing methods for membrane proteins. The sequence of 102 amino acid residues from the NH2 terminus and that of 39 amino acid residues from the COOH terminus of bacteriorhodopsin have been determined. These results are in agreement with those recently published by Ovchinnikov and coworkers [Ovchinnikov, Y.A., Abdulaey, N.G., Feigina, M.Y., Kiselev, A.V. & Lobanov, N.A. (1977) FEBS Lett. 84, 1-4]. Chymotryptic cleavage of bacteriorhodopsin produced two fragments, C-1 (Mr 19,000) and C-2 (Mr 6900), the latter containing the blocked NH2 terminus (pyroglutamic acid). Further fragmentation with CNBr gave mostly hydrophobic fragments, which were separated by gel permeation and reverse-phase high-pressure liquid chromatography in formic acid/ethanol/water mixtures. The fragments were sequenced by a judicious combination of mass spectrometric peptide sequencing and automated Edman degradation. The C-2 fragments were ordered on the basis of methionine-containing peptides identified by gas chromatographic mass spectrometry, while C-1 and C-2 were arranged by analysis of an overlapping CNBr fragment.


Coupled enzyme systems in a vesicular membrane: oxidative phosphorylation as an example. We consider a small vesicle whose membrane transports a ligand L into the vesicle through enzymatic units of type A and transports L out of the vesicle through units of type B. Oxidative phosphorylation in mitochondria provides an example, in which L is H+. The kinetics of the two membrane systems (A and B) are coupled through the concentration of L in the vesicle. This interdependence causes the combined membrane system (A plus B) to simulate a single system whenever the net ligand transport into the vesicle is zero. For example, in oxidative phosphorylation, it was thought for some time that ATP was synthesized by the respiratory chain system (via an "active intermediate"). We give the simplest possible analyses of this kind of coupled system, which is very common, by using two-state enzymes for both A and B above. A numerical example is included that illustrates respiratory control in a qualitative way: although the respiratory chain flux by itself does not depend on ADP concentration, the steady-state flux of the coupled systems (respiratory chain and reverse ATPase) does depend on ADP concentration through the interior ligand (H+) concentration.


Steroid diamine-nucleic acid interactions: partial insertion of dipyrandium between unstacked base pairs of the poly(dA-dT) duplex in solution. We report on an NMR investigation of steroid diamine.nucleic acid complexes as a function of phosphate-to-drug ratios in aqueous solution in order to evaluate the structural and kinetic aspects of the binding of a nonintercalative drug to a synthetic DNA in solution. The nonexchangeable proton chemical shift parameters for the dipyrandium.poly(dA-dT) complex demonstrate unstacking of base pairs and partial insertion of the steroid diamine at the complexation site. The chemical shifts and linewidths of the exchangeable protons as a function of pH demonstrate that the base pairs are intact but partially exposed to solvent at the steroid diamine binding site. The phosphorus chemical shifts suggest that the base pairs unstack upon complex formation without changes in the omega,omega' polynucleotide backbone torsion angles. The NMR line shape parameters require rapid exchange of the steroid diamine among potential binding sites and are consistent with greater segmental flexibility in the complex compared to the synthetic DNA in solution. The NMR experiments are discussed in relation to Sobell's proposed model [Sobell, H.M., Tsai, C.C., Gilbert, S.G., Jain, S.C. & Sakore, T.D. (1976) Proc. Natl. Acad. Sci USA 73, 3068-3072] for the steroid diamine-DNA complex.


Isolation of a 7S particle from Xenopus laevis oocytes: a 5S RNA-protein complex. Previtellogenic oocytes of Xenopus laevis contain a free 5S RNA-protein complex sedimentating at 7 S. This particle consists of one molecule of 5S RNA and one 45,000-dalton protein. The protein of the 7S particle and the protein that is released in association with 5S RNA when the ribosome is treated with EDTA are unrelated. Because the 5S RNA accumulated by small oocytes in storage particles is incorporated into the ribosome later in oogenesis, we conclude that 5S RNA is succesively associated with two proteins during the life span of the oocyte.


Differences in intracellular distribution of plasminogen activator in growing, confluent, and transformed 3T3 cells. The intracellular distribution of plasminogen activator in growing, confluent, and transformed 3T3 cells was investigated by using sucrose density gradient centrifugation. In confluent cells, which do not release plasminogen activator extracellularly, most of the activity was found in a heavy membrane fraction. In growing and transformed 3T3 cells, which do release plasminogen activator extracellularly in large amounts, most of the activity was found in lighter, plasma membrane-enriched fractions. A correlation between its presence in the light membrane fractions and the ability of the cells to release the plasminogen activator extracellularly is made.


Conformational properties of the acetylcholine receptor as revealed by studies with constrained depolarizing ligands. Conformational aspects of the acetylcholine receptor (AcChoR) of Electrophorus electricus have been examined by studies of its interaction with structurally related, constrained aromatic bis quaternary compounds. Among the compounds synthesized was 3,3'-bis[alpha-(trimethylammonium)-methyl]azobenzene dibromide (3,3'-bisQ). This compound is photochromic and can exist in a cis or trans isomeric form, both of which have now been isolated in pure form. Trans-3,3'-bisQ is the most potent activator known, producing a 60-mV depolarization at 0.2 muM and 50% activity at 0.06 muM. The cis isomer is less than 1% as active. Its high activity and constrained structure suggest that trans-3,3'-bisQ can be considered to be a "template" of the combining site of AcChoR, when the latter is in the activated state. The following conclusions can then be drawn concerning the AcChoR binding site. (i) Depolarization can occur by interaction with reagents that are essentially inflexible. (ii) The binding site has a planar hydrophobic region that interacts with methylene groups of acetylcholine and with hydrophobic areas in general. (iii) In the same plane as the hydrophobic area is a site that interacts with electron-donating functional groups including the carbonyl oxygen of acetylcholine and the azo nitrogens of trans-3,3'-bisQ. (iv) About 1.5 A out of the plane of the hydrophobic and the electron acceptor site is an anionic site; when the AcChoR is in the activated state, this site is separated from the electron acceptor site by 5.2 A and from another anionic site by 11 A. (v) The anionic sites are located within a cleft of limited size, sufficient to accommodate quaternary methyl groups. (vi) Although depolarization can occur with reagents that possess only hydrophobic and cationic groups if their geometric arrangement is proper, the highest activity resides in compounds capable of all of the interactions cited above.