Synaptonemal complex karyotyping in Melanoplus differentialis. The chromosomal axes of the spermatocytes of the grasshopper Melanoplus differentialis have been studied with a modification of the microspreading procedure used previously. The whole complement of synaptonemal complexes (SCs) and the axis of the X chromosome have been described and measured. The relative length of each SC is characteristic and constant and permits the construction of an idiogram. Relative lengths of SCs are almost equal to the relative lengths of mitotic chromosomes of spermatogonia (with the exception of the X chromosome), thus extending to an invertebrate the relationship between SCs and mitotic chromosomes that has been demonstrated in mammals. All the SCs except the 3 smallest (which are apparently telocentric) show a small short arm beyond the kinetochore. The progression of changes in the chromosomal axes during meiotic prophase has been staged by centriolar behaviour. During leptotene, axes are first formed near the nuclear envelope at a special (polar) region. SCs also begin to appear in the polar region and extend towards the nuclear interior. The beginning and completion of synapsis is not synchronous among bivalents. The X-axis is formed in midzygotene and shows a characteristic sequence of changes in shape during pachytene. Cells in post-synaptic stages show whole chromosome complements with characteristic chiasmatic configurations. Kinetochores are prominent and bipartite during diplotene-diakinesis.


In vitro transcription of RNA in nuclei, nucleoli and chromatin from physarum polycephalum. Methods for isolating nuclei, nucleoli and chromatin from Physarum polycephalum which retain high levels of endogenous RNA polymerase activity are described. Under carefully controlled conditions with respect to mono- and divalent cation concentrations RNA synthesis in nuclei displayed linear kinetics for at least 30 min and the RNA products had a similar size distribution to nuclear RNA synthesis observed in vivo. Chromatin showed 60% of the nuclear transcriptional activity but no conditions were found where faithful transcription of the template occurred. Isolated nucleoli were 5-fold more active than nuclei and the endogenous RNA polymerase activity was insensitive to alpha-amanitin. Under carefully controlled conditions, the nucleoli appeared to support the accurate transcription, re-initiation and processing of rRNA chains in vitro.


Visualization of interphase chromosomes. Using a modified Giemsa-banding technique we have observed what appear to be chromosomes during interphase in nuclei from Allium cepa root tips and Chinese hamster cells (CHO line). During telophase through G1 chromosomes progressively uncoil and decondense. During S chromosomes are comparatively decondensed, but some segments have structure similar to chromosomes in G1 and G2. During G2 the chromosomes progressively recondense and coil in apparent preparation for prophase. Although specific structural modifications of chromosomes occur in G1, Sand G2 nuclei, chromosomes appear never to decondense to the point that they lose their 3-dimensional integrity, but remain in distinct domains throughout interphase.


Electron-microscopic autoradiography of tritiated testosterone in rat testis. The feasibility of a technique for autoradiography of diffusible substances has been further tested by analysing the localization of steroids in rat testes with the light-and electron-microscope. Testes of rats were perfused with tritiated testosterone (3 min) followed by 15-min perfusion with buffer containing a 100-fold of unlabelled testosterone. Tissue samples were frozen, freeze dried, fixed in osmium vapour and embedded in Epon. To exclude extraction of steroids, contact with water and other solvents was prevented during cutting of thin sections on an ultracryotome and further treatments for autoradiography. Light- and electron-microscopic observations indicate that the highest concentration of labelled testosterone was present within the basal parts of the Sertoli cell cytoplasm and in lipid inclusions of Sertoli cells within the seminiferous tubules. This is the first account of autoradiography of steroids at the electron-microscope level.


[Free intra-peritoneal endometrioma associated with ileal and appendicular endometriosis (author's transl)]. The authors report three rare localisations of endometriosis: ileal, appendicular and free endometrioma of the pouch of Douglas and study the diagnostic difficulties of digestive endometriosis and the interest of routine histological examination of structures found apparently free in the pouch of Douglas.


[Effects of stress and highly selective vagotomy on mast cells in the wall of the rat stomach (author's transl)]. Quantitative changes in the gastric mast cells in ulcer diathesis and especially in the development of acute ulceration have already been studied. Qualitative changes may be asse-sed by Alcian Safranin blue stain which permits one to distinguish two sub groups: the blue mast cells and the red mast cells, which alone undergo degranulation. This report describes the modifications in number and aspect of the mast cells of the stomach of the white rat after stress due to restraint at variable intervals after highly selective vagotomy. The density of the mast cells and their appearance depend partly on vagal nerve supply. After highly selective vagotomy, their density increases while the initially unbalanced proportion of red and blue mast cells becomes normal in the long term. After stress, there is a reduction in the number of mast cells and, above all, disappearance of the red forms whether the rats are vagotomised or not. This study takes into consideration multifactorial problems which intervene in the etiology of stress ulcers and the necessity of continuing the study of the consequences of vagotomy.


Decrease of prolactin by methysergide in amenorrheic hyperprolactinemic women. The effect of methysergide (MES), a serotoninergic antagonist on prolactin (PRL) blood levels was studied in five hyperprolactinemic amenorrheic women. The drug was administered for five days at a daily dosage of 11.2 mg. MES decreased significantly the PRL blood levels in all subjects (p less than 0.01). Since the MES has been shown to have antiserotoninergic effects and since serotonin has been thought to be involved in the control of PRL release, the effects of MES in lowering PRL might be due to a decrease of serotonin tone.


Galactorrhea and hyperprolactinemia associated with chest wall injury. A 48 year old premenopausal woman presented with galactorrhea and amenorrhea associated with chest wall burns. Basal serum prolactin levels were raised, and were further elevated by the administration of L-dopa, chlorpromazine and TRH. Intercostal nerve block and bromocryptine treatment reduced prolactin levels to normal, but did not noticably reduce milk secretion.


Periodicity and self-actualization in women. Studied the relationship between menstrual cycle phase and self-actualization as measured by the Personal Orientation Inventory (POI). In classroom testing sessions, 160 high school students completed the POI and personal data sheets, which gave information about their menstrual cycle phase. Premenstrual, menstrual, and midcycle groups of 20 Ss each were formed from those girls eligible for inclusion in each condition. Three one-by-three analyses of variance were run using the TC, I, and TC + I scores of the POI, which were selected as the best overall measures of self-actualization. A significant (p less than .05) difference on TC + I was found; subsequent t-tests revealed that the menstrual group's TC + I score was significantly (p less than .02) higher than that of the midcycle group. The difference between menstrual and premenstrual Ss on TC + I, though not significant, lay in the same direction and was of the same order of magnitude. Possible explanations for the results and implications for future testing that involves females are presented briefly.


3H estradiol in catecholamine neurons of rat brain stem: combined localization by autoradiography and formaldehyde-induced fluorescence. 3H setradion-17beta was localized in nuclei of catecholamine (CA) neurons of the rat lower brain stem, i.e., midbrain, pons and medulla, by employing a combined formaldehyde-induced fluorescence (FIF)-autoradiography approach which permits the direct visualization of 3H estradiol uptake in relation to CA-neuron perikarya and terminals. In the medulla, noradrenalin (NA)-containing neurons in and adjacent to the nucleus (n.) reticularis lateralis (group A1) and in the vicinity of the n. tractus solitarii (group A2) concentrated 3H estradiol. In the pons, NA neurons dorsal to the n. olivaris superior (group A5), in the locus ceruleus (group A6) and ventral to the pedunculus cerebellaris superior (group A7) displayed 3H estradiol uptake. In the midbrain, dopamine (DA)-containing neurons in the n. cuneiformis (group A8), in the zona compacta of the substantia nigra (group A9) and adjacent to the n. interpeduncularis (group A10), were not seen to concentrate 3H estradiol. This is in contrast to DA neurons in the n. arcuatus of the hypothalamus (group A12), previously reported to concentrate 3H estradiol. In addition, certain non-CA-containing neurons with nuclear uptake of 3H estradiol were observed to be surrounded by CA terminals in such areas as substantia grisea centralis, n. tractus solitarii and n. tractus spinalis nervi trigemini. This suggests a second mode of interaction between CA neurons and estrogen target neurons. The estradiol-CA neuron relationships described may provide morphological substrates underlying functional interactions between estradiol and CA neurons in the rat brain.


Changing meal patterns and suppression of feed intake with increasing amounts of dietary nonprotein nitrogen in ruminants. Goats were injected intraruminally during spontaneous meals with ammonium chloride, urea, ammonium lactate, or sodium lactate arranged in a Latin square experimental design randomized for order of treatments. Urea and ammonium injections shortened meal length by 20 to 30%. Rate of eating and meal frequency were reduced. Sodium lactate injections reduced meal size. In cows, meal length and meal size also were measured. Grain concentrate, corn silage, and chopped hay were fed as complete mixed rations. In the concentrates 58+ of the nitrogen was either from soybean meal or urea. Length of the first meal after feeding was reduced from 24.3 min with soybean meal to 12.4 with urea. Meal size was reduced from 3.2 kg to 1.8 kg when urea was fed. Total feed intake was similar, 12.0 kg/day (soybean meal) and 11.6 kg/day (urea) since spontaneous meal number and size were 17 and .30 kg for soybean meal but increased to 23 and .36 kg for urea. The physiological basis for the limit on meal length with urea rations is unknown but is an important factor in successful feeding of urea when eating time is limited for cows.


Vitamin A transport in plasma of the non-mammalian vertebrates: isolation and partial characterization of piscine retinol-binding protein. Studies were conducted to explore vitamin A transport in the non-mammalian vertebrates, especially Pisces, Amphibia, and Reptilia, and to isolate and partially characterize piscine retinol-binding protein. Retinol-containing proteins in fresh plasma obtained from bullfrogs and a turtle exhibited similar properties to those found in mammalian and chicken plasma: i.e., molecular weight of about 60,000-80,000 as estimated by gel filtration and binding affinity to prealbumin on human prealbumin-Sepharose affinity chromatography. In sharp contrast, vitamin A-containing proteins in plasma from larvae of bullfrogs as well as three fishes (carp, blue sharks, and young yellowtails) appeared to be present in plasma as monomeric retinol-binding proteins without any affinity to human prealbumin. On the other hand, plasma vitamin A in the lamprey (Cyclostomes) was found to exist exclusively as an ester form in association with the lipoproteins of hydrated density less than 1.21 g/ml. Piscine retinol-binding protein was isolated from pooled plasma of young yellowtails and was converted (1000-fold purification) to a homogeneous component by a procedural sequence that included gel filtration on Sephadex G-100, chromatography on SP-Sephadex, gel isoelectric focusing, and, finally, polyacrylamide gel electrophoresis. Purified piscine retinol-binding protein showed physico-chemical properties distinctly different from the mammalian and chicken retinol-binding proteins examined, i.e., a smaller molecular weight of approximately 16,000, a lower isoelectric point of 4.3, a prealbumin mobility on analytical polyacrylamide gel electrophoresis, and a lack of binding affinity for human prealbumin; however, it displayed similar characteristics in two ways: a 1:1 molar complex with retinol, and a high content of tryptophan (four residues). These results strongly suggest that the piscine retinol-binding protein is a prototype of the specific vitamin A-transporting protein in plasma of the vertebrates, being modified later in evolution, during phylogenetic development of the vertebrates, to acquire a binding site for prealbumin on the molecule.


A large-scale purification of phosphatidylethanolamine, lysophosphatidylethanolamine, and phosphatidylethanolamine, and phosphatidylcholine by high performance liquid chromatography: a partial resolution of molecular species. Egg yolk phospholipids, on a 10 g scale, were resolved by high-performance liquid chromatography on an 8-m silica column with elution by a stepwise chloroformmethanol gradient into homogenous phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, and lysophosphatidylethanolamine fractions. Within these fractions, partial resolution on the basis of fatty acyl side chain composition was achieved.


Characterization of the alkyl ether species of phosphatidylcholine in bovine heart. The molecular types of lecithin in beef heart lipids were investigated by a combination of mild hydrolytic procedures and silicic acid chromatography. The major species of sn-glycero-3-phosphorylcholine (GPC) were found to be: diacyl-GPC (57%), alk-l-enyl, acyl-GPC (39%), alkyl acyl-GPC (3%), and dialkyl-GPC ( less than 1%). No di-alk-l-enyl-GPC or alk-l-enyl alkyl-GPC were detected. The derived monoalkyl-and dialkyl-glycerols were characterized by their infrared spectra and alkyl chain compositions.


Amoeboid movement as a correlated walk.

Amoeboid movement as a correlated walk. The movement of slime-mold amoebae under isotropic conditions in two dimensions is represented as a Correlated Walk with straight steps of variable length. The steps are correlated via the angle theta turned through from step to step. The hypotheses and predictions of the model compare favourably with experimental data for Dictyostelium discoideum amoebae.


Metabolism of [14C]testosterone by human foetal and adult brain tissue. The metabolism of [14C]testosterone in vitro by various areas of the human foetal brain has been studied and compared with that of adult brain. The predominant metabolites were 5alpha-dihydrotestosterone and 5alpha-androstane-3alpha, 17beta-diol, and also androstenedione, and all areas of the foetal brain showed similar activity. In the foetal pituitary gland, the activity of 5alpha-reductase was less prominent than that of 17beta-hydroxysteroid-dehydrogenase. Small quantities of oestradiol-17beta were produced from testosterone by the hypothalamus, temporal lobe and amygdala only, and no aromatization could be detected in the pituitary gland. 5alpha-Reductase activity was much lower in adult brain tissues and no oestradiol was identified in adult temporal lobe tissue.


Effects of oestradiol and progesterone on the induction and duration of sexual receptivity in cyclic female rats. Intact 4-day cyclic rats showed sexual receptivity 24 h after an injection of oestradiol benzoate (OB) on any day of the cycle except on the second day after the display of spontaneous oestrus. Ovariectomy at the time of OB treatment abolished the behavioural response, but receptivity was restored by progesterone. Progesterone treatment early on the day of behavioural oestrus advanced the display of receptivity but did not affect the time at which oestrus ended. Repeated treatment of sexually receptive rats with progesterone did not affect the duration of oestrus. These results show that sexual receptivity in the intact rat cannot occur in the absence of oestradiol and progesterone. The results further suggest that progesterone may not be associated with the mechanisms terminating behavioural oestrus in rats. Treatment with OB on the day of oestrus can prolong the duration of receptivity but only at a higher dosage than that needed for induction of receptivity.


Alterations in plasma gonadotrophin patterns caused by sodium pentobarbitone in ewes at oestrus and in anoestrous ewes after infusion of oestradiol. A radioimmunoassay system for the measurement of ovine follicle-stimulating hormone (FSH) was established. After the onset of normal oestrus, there were simultaneous surges of luteinizing hormone (LH) and FSH, and 24 h later, a surge of FSH alone. Administration of sodium pentobarbitone when the dual surge of LH and FSH was expected inhibited the release of LH, but prolonged that of FSH. Development of corpora lutea that took place immediately was normal in ewes treated with sodium pentobarbitone, but after the subsequent oestrus, the life-time of corpora lutea was abnormally short. When sodium pentobarbitone was administered at the time when the second surge of FSH was expected, no effect was observed on the level of LH or FSH. Subsequent development of corpora lutea was similar to that in the non-treated group. Administration of sodium pentobarbitone at the expected time of the dual surges of LH and FSH after infusion of oestradiol to anoestrous ewes blocked the release of both LH and FSH. Administration of sodium pentobarbitone at the expected time of the second surge of FSH after infusion of oestradiol delayed the increase in the level of FSH. In a pilot experiment after combined infusion of androstenedione and oestradiol, sodium pentobarbitone did not completely inhibit the release of FSH, but the release of LH was totally prevented.


Punishment-specific effects of pentobarbital: dependency on the type of punisher. Pigeons were trained to peck a key under a multiple random-interval 1-minute, random-interval 6-minute schedule of food presentation. Subsequently, over three phases, additions were made during the random-interval 1-minute component as follows: pecks during the component occasionally were punished by timeout presentation (Phase 1), timeouts were presented independently of responding during the component (Phase 2), pecks during the component occasionally were punished by electric-shock presentation (Phase 3). In Phases 1 and 3, response-dependent timeout and shock suppressed responding and established equivalent rates in both components of the multiple schedule. Intermediate doses of pentobarbital increased responding suppressed by electric-shock punishment but had little or no effect on responding suppressed by timeout punishment. Response-independent presentation of timeouts did not result in suppression of responding (thus showing that response-dependent timeout acted as a punisher), and pentobarbital did not reliably increase unpunished responding. Pentobarbital's selective "punishment-attenuating" properties depend on the nature of the punisher.


Regulation of reaginic antibody production in mice. I. Suppression by antigen of IgE antibody production in vitro. Regulation of IgE production by antigen in a primed murine splenic lymphocyte culture system was described. Maximum IgE antibody production was found to occur when cells were cultured in the absence of exogenously added antigen. A cells and T lymphocytes did not affect the production of anti-DNP IgE antibody. By using a hapten-carrier antigen system (DNP-EA) for priming mice in vivo, it was found that the production of anti-DNP IgE by spleen cells in vitro was inhibited by hapten when coupled to homologous (EA) or heterologous (BGG) carrier, and was not enhanced or inhibited by homologous carrier. Anti-DNP IgE antibody production by cultures depleted of macrophages or T lymphocytes was found to be as sensitive to the suppressive effects of hapten as was the IgE production by whole spleen cell cultures. Both IgM and IgG secondary anti-DNP PFC responses in vitro were enhanced by the presence of the homologous hapten-carrier or carrier alone. DNP-BGG had no effect on the anti-DNP IgM or IgG PFC responses of the cultures. These data suggest that endogenous production of antibody (IgM or IgG) was not responsible for the observed suppression of the IgE response in vitrol The experimental results presented indicate that the regulation of the IgE production by antigen in the primed mouse splenic lymphocyte cultures was a consequence of the direct interaction of hapten with IgE B cells.


[Determination of iodine in plasma and biological fluids in the range 2 nmol/l with the aid of an autoanalyser (author's transl)]. An automated continuous flow method is described for the determination of iodine in biological fluids with a detection limit of 0.4 nmol/l. Our method uses the kinetics of the Sandell-Kolthoff-Reaction in sulfuric acid for the determination of iodide. The following applications are described: plasma inorganic iodine, total plasma iodine, protein bound iodine, total iodine in urine, drinking water and milk, total iodine in vegetables and laboratory diet for rats.


In vitro culture of growing mouse oocytes. A method is described for in vitro culture of naked growing mouse oocytes for at least four days. Tests for metabolic function indicate that the oocytes increase in volume, accumulate glucose-6-phosphate dehydrogenase, and maintain a steady level of 3H-uridine and 3H-leucine incorporation.


Production of Friend leukaemia antigens in chronically-infected cells treated with interferon. The effect of mouse interferon (ITF) on the expression of Friend leukaemia virus (FLV) an on dimethyl sulphoxide (DMSO)-stimulated haemoglobin synthesis in Friend erythroleukaemic cells (FLC) was studied. Immunofluorescent staining was used to detect intracellular antigens, and incorporation of 3H-uridine into virions to detect extracellular virus release. Interferon markedly inhibited haemoglobin synthesis and FLV production, but enhanced accumulation of virus antigens in the cytoplasm; on the cell surface, however, FLV antigens were present to the same extent whether ITF was present or not. When ITF was removed, virus production rose and intracellular virus antigens fell to the levels of untreated controls.


RNA synthesis directed by a temperature-sensitive mutant of Semliki Forest virus. RAN synthesis induced by a temperature-sensitive RNA negative mutant, ts-4, of Semliki Forest virus was studied both at the permissive (28 degrees C) and restrictive temperature (39 degrees C). At 28 degrees C ts-4 directed RNA synthesis was qualitatively similar to the wild type but overall synthesis remained somewhat lower. Only about 3% of RNA was synthesized compared to the wild type virus when ts-4 was maintained at 39 degrees C throughout the infection. The sedimenting radioactivity was almost exclusively in 42S RNA. When ts-4 infected cultures were shifted from 28 to 39 degrees C at 5 h post-infection, the synthesis of 26S RNA was shut off while 42S RNA synthesis continued. The synthesis of 26S RNA started again even in the presence of cycloheximide if the cultures were back to 28 degrees C. Our results suggest that 26S RNA synthesis is controlled by a virus-specific protein, the function of which is not required for the synthesis of 42S RNA, In ts-4 this protein is denatured at 39 degrees C and becomes non-functional, but renatues and becomes functional upon shift down to 28 degreesC.


African swine fever virus replication in porcine lymphocytes. Purified preparation of porcine lymphocytes were infected with three isolates of virulent African swine fever virus (ASFV). Electron microscopy showed the presence of small numbers of mature virus particles in degenerating cells. The titres of infective virus released were low and reached a maximum by 24 h after infection.


Manpower for neurosurgery: seeing ourselves as others see us. The 1977 Harvey Cushing oration. The 1977 Cushing Orator looks at the question of neurosurgical manpower and its relation to national health policies, proposed or abandoned. The impact on residency training as well as patient care and research effort require constant monitoring by the profession, so that the specialty will continue to contribute significantly to human betterment.


Experiences with sterilization of the operating microscope. Experiments were conducted to assess the feasibility of both paraformaldehyde and ethylene oxide gas sterilization of the operating microscope. From these experiments and practical experience, it is concluded that ethylene oxide sterilization of the operating microscope is a feasible and desirable alternative to cumbersome draping technique.


Effect of chronic ethanol administration on thiamin metabolism in the rat. The effect of chronic alcohol administration on the absorption, excretion and metabolism of thiamin in the rat has been examined. In ethanol-fed rats receiving a marginal daily intake of thiamin (10 microgram/day) by stomach tube or by intraperitoneal injection, less of the vitamin and its metabolites were excreted in the urine as compared to controls administered the same diet except that sucrose replaced the energy represented by ethanol. More of the oral dose of thiamin was excreted in the feces of the ethanol-fed as compared to control rats. These data support previous reports of decreased adsorption of thiamin from the gut in animals exposed to ethanol. The studies in which the thiamin was administered by intraperitoneal injection also indicate an effect of ethanol on thiamin excretion in the urine which appears not to be related to absorption of the vitamin from the gut. An examination reveals no difference in the level of thiamin and its metabolites in the tissues of ethanol-fed as compared to control rats receiving thiamin by stomach tube. Thus, the decreased absorption of thiamin from the gut in the ethanol-fed rats seems to be balanced by a decreased excretion in the urine leading to a comparable accumulation of the vitamin in the tissues as controls.


Calcitonin therapy of children with osteogenesis imperfecta. Two children, ages 13 2/12 and 6 6/12 years, with osteogenesis imperfecta were treated with salmon calcitonin. During the course of therapy the older child developed calcitonin dose-related hypomagnesemia on two occasions. The younger child, coincident with otitis media and vomiting, developed hypomagnesemia, hypophosphatemia, hyponatremia, and hypokalemia. Since rib biopsies obtained before and after one year of treatment with salmon calcitonin failed to demonstrate any histologic changes, therapy was discontinued because of the induced metabolic consequences of calcitonin therapy.


Feeding behaviour in rats with isolated medial hypothalamus as a function of ambient temperature. The experiments of mechanical isolation of medial hypothalamus from the lateral hypothalamus and the preoptic anterior hypothalamic (POAH) region in rats showed that: 1. The interruption of neural connections between POAH area and medial hypothalamus do not prevent the decrease of food intake which normally occur in a hot environment. 2. At 33 degrees C, hyperphagic rats gained more weight than sham-operated ones. 3. At 4 degrees C, rats made hyperphagic by hypothalamic isolation do not ajust their food intake for a long period and do not gain weight. 4. The excitatory pathways of the feeding center from the POAH area do not penetrate directly into the lateral hypothalamus, but rather into the medial retrochiasmatic area. 5. The temperature influences the diurnal pattern of feeding only in rats with intact or unilateral neural connections of the hypothalamic structures 6. It seems that the thermostatic mechanism, which is a potent regulator of feeding, is closely associated with the central control of thyrotropin release, and that the hypothalamic structures may be considered only as a necessary link in the nervous mechanism involved in feeding control.


Hemolytic anemias during pregnancy and the reproductive years. Anemia is a common phenomenon in women during the reproductive years. In pregnancy, it is associated with an increased incidence of maternal-fetal morbidity and mortality. The approach to the investigation of anemic women suspected of having hemolytic anemia of either congenital or acquired etiology is the subject of this article. Various conditions in the pregnant women can have hematologic consequences for the newborn infant; these conditions include sensitization to fetal blood cells, infections, drug ingestion and the possession of genes for hereditary hemolytic disorders, which may be transmitted to the fetus. Because several forms of hemolytic anemias are hereditary or are caused by an altered gene, genetic consultation is important.


Cerebral dopamine agonist properties of some 2-aminotetralin derivatives after peripheral and intracerebral administration. A series of variously N-substituted 2-aminotetralins having OH groups at 5 and 6 and at 6 and 7 positions, as well as nonoxygenated systems, has been evaluated for central dopaminergic effects. Stereotypical behavioral effects (sniffing, compulsive gnawing, and hyperactivity) produced by direct intracerebral administration of some of the agents were shown to differ strikingly from responses resulting from peripheral administration. The centrally mediated responses of hyperactivity and sterotypical gnawing-biting head and limb movements were shown to be separable in some test compounds. An improved route to 2-aminotetralin systems has been utilized for some of the compounds, which involves Pummerer rearrangement and cyclization of beta-keto sulfoxides and reductive amination of beta-tetralones with a NaBH4-carboxylic acid complex.


Congenital discoid lupus in the newborn. A female infant, born to a 21-year-old mother with systemic lupus erythematosus, had cutaneous discoid lupus at birth. The lesions resolved spontaneously over the first few months and by the age of 1 year the infant's skin was normal. Other possible complications of this maternal disease are discussed and the need for caution in counselling mothers is recommended.


Test for malignant transformation of rat liver cells in culture: cytology, growth in soft agar, and production of plasminogen activator. Three parameters were evaluated as diagnostic of the malignant potential of cultured rat liver epithelial cells: cytology, growth in soft agar, and production of extracellular plasminogen activator. A total of 22 tumorigenic and nontumorigenic cultures from 15 cell lines were sent coded from their originators to two different laboratories for the evaluation of these three parameters. Cytologic diagnosis and growth in soft agar were reliable means of determining the malignant potential of the cultured cells. However, the production of extracellular plasminogen activator showed little correlation with tumorigenicity. Of cytologic properties evaluated, the two that correlated best with malignant potential were increased cytoplasmic basophilia and and increased nuclear:cytoplasmic ratio.


Excess chromosome no. 4 in ethylnitrosourea-induced neurogenic tumor lines of the rat. The chromosomes in 15 cell lines derived from separate tumors induced in rats by ethylnitrosourea (ENU) are described. Thirteen lines were neural (glioma or schwannoma) in origin and type. In 12 of these lines, excess chromosome no. 4 could be demonstrated by Giemsa banding. One to three extra no. 4 chromosomes were seen as numerical or structural abnormalities. Also noted were other changes that were not consistent among lines. The 12 lines produced tumors in newborn rats. The 13th neurogenic line lacked excess chromosome no. 4 and did not produce tumors. The remaining 2 lines were nonneurogenic and lacked excess chromosome no. 4 but produced tumors. Control studies included chromosome analyses of bone marrow preparations from ENU-treated rats with tumors, cell lines from brains of normal rat embryos, and 2 established nonneurogenic rat tumor lines. No excess chromosome no. 4 was seen. These results suggest that nondisjunction and/or rearrangement of chromosome no. 4 is associated with the oncogenic process in neurogenic tumors induced.


Hormone regulation of growth: stimulatory and inhibitory influences of estrogens on DNA synthesis. Estrogenic hormones first stimulate and then inhibit DNA synthesis in the uterus of the immature rat. Both the stimulatory and the inhibitory effects depend on the sustained presence of estrogen. Thus, estriol, which is equal in effectiveness to estradiol on early (up to 6 hr) responses, has only a partial stimulatory effect on DNA synthesis. Estradiol initially stimulates DNA synthesis, but the sustained presence of this steroid inhibits further synthesis of this macromolecule and cell division. These observations are discussed in terms of their relationship to current models of estrogen action and to estrogen dependency in some types of cancer.


Evaluation of pedal lymphangiography in staging non-seminomatous testicular carcinoma. An analysis is presented on 45 patients undergoing pedal lymphangiography followed by retroperitoneal lymphadenectomy for non-seminomatous testicular carcinoma. Retroperitoneal node metastases were predicted correctly with the lymphangiogram in 10 of 20 patients (50 per cent). The over-all predictive accuracy was 62 per cent.


Prevention of vesicoureteral reflux with Fogarty catheters during formalin therapy. Vesicoureteral reflux during formalin therapy of intractable hemorrhagic cystitis can be prevented with Fogarty catheters inserted cystoscopically into the lower ureters. With this technique formalin instillation and cystography can be done through the cystoscope at the same time. Topical intravesical vasopressin did not, in our experience, lessen the bladder hemorrhage.


[Diurnal rhythm of the adrenal cortex function in patients with circulatory disorders]. The blood hydrocortisone and corticosterone content and the aldosterone level in the urine were determined for 24 hours in patients with congenital heart disease before and after operative treatment. In patients with symptoms of cardiac insufficiency both glucocorticoid and mineralocorticoid activity of the adrenal cortex was marked by deviations from the diurnal physiological rhythm. This is reflected in the continuous activation of the adrenal cortex during 24 hours; activation of mineralocorticoid function in the evening and during the night is a particularly characteristic phenomenon.


[Role of aortic dystrophy in the etiology of idiopathic cardiomyopathy]. Hypertrophy of the heart of unrecognized origin is a frequent finding on autopsy and in that case is related to the so-called nonobstructive idiopathic cardiomyopathy. Abnormality of the heart muscle is considered its main symptom. According to the authors, involvement of the ascending aorta and not the myocardium is the basic pathologic process. This "aortic dystrophy" is characterized by diffuse ectasia of the ascending aorta and typical microscopic histological signs. Hypertrophy of the left heart is evidently overexertion hypertrophy resulting from the increased volume of the ascending aorta and the diminished elasticity of the aortic wall after the ventricular systole and the consequent greater demand on the work of the left ventricle than in normal conditions. "Idiopathic" hypertrophy of the heart with dystrophy of the aorta is hereditary, it may involve the skeleton also, and may be manifested in relatives.


Aplysinopsin: antineoplastic tryptophan derivative from the marine sponge Verongia spengelii. Bioassay-guided fractionation of the aqueous alcoholic extract of the marine sponge Verongia spengelii for tumor inhibitory agents led to the isolation of aplysinopsin, a new tryptophan derivative recently reported from another sponge Thorecta aplysinopsis. Aplysinopsin showed cytotoxicity against the KB, P388 and L1210 cell cultures.


[BCG-osteomyelitis and BCG-arthritis after vaccination with BCG (author's transl)]. After vaccination with BCG (type Goeteborg) in the newborn period two children developed a BCG-osteomyelitis 12 and 19 months respectively later. The symptoms were only small. X-ray showed bone lesions in the distal metaphysis of the femur. The bacteriological examination demonstrated BCG-etiology. Disturbances of growth or of joint motility are seldom; they are possible, if the bone lesions are severe and if the diagnosis is late. In these cases the treatment must be intensive.


Extrapolation of linear attenuation coefficients of biological materials from diagnostic-energy x-ray levels to the megavoltage range. A dual-energy algorithm is used in determining the effective atomic number, atomic density, and electron density of biological substances. These quantities are then used to calculate linear attenuation coefficients at the megavolttage level. The validity of this method is checked several ways, including a comparison of extrapolated values with experimental data reported by Rao and Gregg where linear attenuation coefficients at 60 and 122 keV are used to extrapolate coefficients at 662 keV. Except for a few instances, the extrapolated values agree quite well with the reported experimental values. This method is also used to calculate coefficients at the 60Co range, and these are compared with experimental values measured in water and various types of tissue-equivalent materials. An additional algorithm is developed to extrapolate coefficients in water and bone up to 10 MeV. These quantities are compared with accepted values previously reported in the literature.


A panaeolus poisoning in Scotland.

A panaeolus poisoning in Scotland. A male and female patient were admitted to the Poison Unit, Royal Infirmary, Edinburgh, suffering from poisoning caused by eating basidiocarps of Panaeolus subbalteatus (B. & Br.) Sacc. Those basidiocarps involved in the poisoning and later collections are described in full and pertinent ecological data etc. given.


[Thin-layer chromatographic technic for the semiquantitative determination of hydroxyzine residues in animal material]. The solution behaviour of hydroxyzine in water and chloroform (in the strongly alkaline and the strongly acidic range) is utilized to extract it from animal material and to purify the extract. A concentration of 0.01 p.p.m. is detectable in liver, kidneys and muscular substance. 0.05 p.p.m. may be determined semiquantitatively by visual spot comparison. In milk, the detectable amount is 0.1 p.p.m. and 0.5 p.p.m. can be determined semiquantitatively. The recoveries of low concentrations of hydroxyzine are likely to be lower in milk samples than in organ samples.


Lack of algesic effect of substance P on paravascular pain receptors. 1. Substance P (synthetic or extracted for intestine or central nervous system) is devoid of an algesic effect on paravascular pain receptors. 2. The algesic effect of a AP-containing acetone HCl-extract from spinal cord is explained by its high content of potassium ions. 3. SP-containing preparations which include an ammonium sulphate precipitation in the extraction procedure are algesic due to content of this salt. 4. SP-containing extract from intestine were found to be contaminated with a bradykinin-like peptide of high algesic potency. 5. These findings are discussed with regard to the restricted value of earlier results about central actions of SP-containing tissue extracts and with regard to the role of SP as a possible neurotransmitter.


Diagnostic and therapeutic reevaluation of patients with intractable epilepsy. Intensive monitoring techniques were used to improve seizure diagnosis and control in 23 patients with intractable epilepsy: (1) Video recording of clinical seizures and the ictal EEG, (2) long-term telemetered EEG recordings, and (3) frequent determination of plasma antiepileptic drug concentrations. Patients were monitored in the hospital for an average of 8 weeks and after discharge were followed for an average of 8 months. At follow-up, 70 percent of the patients continued to have improved seizure control as compared with baseline studies, 83 percent had decreased toxicity, and about half had made gains in social adjustment. Use of the techniques described can make a significant contribution to patients previously considered intractable to therapy.


A new juvenile hexosaminidase deficiency disease presenting as cerebellar ataxia. Clinical and biochemical studies. A boy with mild hand tremor since age 2 1/2 was found at 4 to have cherry-red spots and mild trucal ataxia without seizures or dementia. Biochemically, he had striking hexosaminidase deficiency (serum: 4.6 percent of normal, 88.9 percent heat-labile; leukocyte: 2.2 percent of normal, 84.6 percent heat-labile; fibroblast 12.8 percent normal, 93.1 percent heat-labile). The residual hexosaminidase activity migrated electrophoretically in two bands. The major band comigrated with hexosaminidase A, the minor with hexosaminidase S. Hexosaminidase B was totally absent. The parents had partially reduced hexosaminidase with a decreased heat-stabile fraction. This disorder may result from a new mutation closely related to that causing Sandhoff-Jatzkewitz disease.


Acetylcholine receptors of aneurally cultured human and animal muscle. We describe the diffuse nonjunctional distribution of AChR molecules of aneurally cultured human and animal muscle and the influence of sera from myasthenia grivis patients and rabbits with experimental autoimmune myasthenia gravis on binding of alphaBT to diffuse nonjunctional AChR. One-hour incubation of myasthenia gravis seria resulted in blocking of the alphaBT-immunoperoxidase staining of their AChRs, while incubation in normal sera did not. Aneurally cultured muscle can aid studies of regenerating fibers in normal muscle compared with those of muscles in neuromuscular diseases, and also act as an environmentally controlled test object for demonstrating the effect of circulating pathogenic factors.


Calcification of basal ganglia and cerebellar roof nuclei in mentally defective patient with hidrotic ectodermal dysplasia. Analysis of intracranial concretions by electon microprobe. This report describes, for the first time, an analysis by electron microprobe of concretions in the brain of an individual with striopallidodentate calcification. We also report the unique association of this intracranial syndrome with hidrotic ectodermal dysplasia. An institutionalized male with impaired intellectual function and hidrotic ectodermal dysplasia was known since the age of 3 years to have bilateral radiopaque densities in the region of the basal ganglia on skull roentgenogram. He died at age 29 in congestive heart failure from rheumatic pancarditis. At autopsy, concretions were identified in globus pallidus, caudate nuclei, thalamus, and dentate nuclei. Mineral deposits within the brain, analyzed by energy dispersive x-ray microanalysis, consisted predominately of calcium and phosphorus. Trace amounts of magnesium, iron, and silicon also were detected.


Spinal cord compression due to pseudomonas in a heroin addict. Case report. Involvement of the spinal cord has not been reported in osteomyelitis of the spinal column caused by Pseudomonas aeruginosa. Cerebrospinal fluid findings have been reported only once in osteomyelitis of the spine by this agent. Our patient had persistently increased cerebrospinal fluid protein during an acute episode of cervical spine osteomyelitis, characterized by fever and neck pain. Roentgenograms of the cervical spine and neurologic examination did not show any definite abnormality. He became paraplegic 18 months later, after having symptoms of numbness of the feet and progressive weakness of the legs for 1 month. A surgical procedure and antibiotic treatment resulted in remarkable recovery. A bone specimen grew P. aeruginosa.


Astrocytoma in three sisters.

Astrocytoma in three sisters. Three sisters died of astrocytoma, and the microscopic appearance of the tumor was the same in all three. This finding supports either genetic or environmental influences in the occurrence of brain tumors.


Diffuse "anoxic" myelopathy.

Diffuse "anoxic" myelopathy. Pathologic changes and distribution of lesions of the spinal cord were studied in 16 patients who suffered from "anoxic" episodes. The lesions were symmetrical and limited to the gray matter. The vulnerability of the spinal cord was most marked in the lumbosacral region, although almost every nucleus throughout the spinal cord was subject to damage.


Benign epileptiform transients of sleep. Clarification of the small sharp spike controversy. Electroencephalograms were performed on 120 normal subjects sleep deprived for 24 hours. Twenty-four percent of the volunteers exhibited one or more epileptiform transients during stages 1 or 2 of non-rapid eye movement sleep. These potentials usually appeared as monophasic or diphasic spikes unaccompanied by sharp waves or focal slowing. When abundant, they occurred sporadically and independently over both hemispheres but were best developed in the anteromesial temporal regions. A 20 percent incidence of similar spikes was found in 599 consecutively referred patients recorded under the same conditions. We conclude that these epileptiform transients of sleep, which have been called "small sharp spikes," are normal and are of no diagnostic value in the evaluation of patients with seizures.


Computed tomography (CT) in the diagnosis of intracranial abscesses. Brain abscess, subdural empyema, and epidural empyema. Nine cases of brain abscess, five of subdural empyema, and one of epidural empyema with associated subdural empyema were identified using computed tomography (CT). Smal and multiple abscesses as well as unilateral, bilateral, and parafalcine empyemas were detected. These lesions were demonstrated rapidly and accurately, even in areas that are demonstrated poorly by other diagnostic techniques. No false-negative studies were found in cases of brain abscess or subdural empyema. However, since the appearance of brain abscesses of CT scans is similar to that of neoplastic and vascular lesions, false-positive reports of brain abscess were encountered.


Postural changes, tremor, and myoclonus in the rat immediately following injections of p-chloromaphetamine. A single dose of p-chloroamphetamine, 10 mg per kilogram, produced postural abnormalities, tremor, myoclonus, and autonomic signs in rats 5 minutes after intraperitoneal injection. This syndrome lasted 60 to 90 minutes, and its intensity was directly proportional to the amount of p-chloroamphetamine given over a 2 to 10 mg per kilogram range. Whole-brain levels of serotonin and 5-hydroxyindoleacetic acid were not altered during this interval, although both were reduced significantly 1 day later. Pretreatment with drugs that interfere with the uptake of p-chloroamphetamine into terminals of serotonergic neurons (fluoxetine), depress brain serotonin levels (p-chlorophenylalanine), or block serotonin receptors (methiothepin or methergoline) suppressed this syndrome, whereas drugs that antagonize the effects of dopamine, norepinephrine, and acetylcholine did not. These observations implicate serotonergic mechanisms and provide behavioral evidence of p-chloroamphetamine's immediate actions on serotonergic neurons in the central nervous system.


Reflex effects of vibration in patients with spinal cord lesions. The vibration reflex was studied in 49 patients with traumatic spinal cord lesions. It was elicited in all patients, even after presumably complete division of the cord. The vibration relfex consisted of a short-latency, brief outburst of phasic activity of motor units, followed by rapidly decreasing phasic component and a later slowly declining tonic component. When periods of vibration were repeated at short intervals of 2 to 10 seconds, the responses showed an approximately exponential decline, although the beginning of each subsequent response was always larger than the end of the preceding response. A large part of this decline can be characterized as a habituation of the vibration reflex. In comparison with the vibration reflex in normal subjects, the phasic component was increased and the tonic one reduced. The tonic component was especially susceptible to potentiation and dishabituation by voluntary effort to contract the vibrated muscle, even in some patients with no other evidence that the lesion was incomplete. We suggest that the tonic component of the human vibration reflex depends, at least in part, on segmental interneurons and their descending spinal pathways, while the phasic component depends mainly on the excitability level of spinal motoneurons.


Erythrocyte survival in Duchenne muscular dystrophy. Hemolysis was investigated in seven patients with Duchenne dystrophy. No increase in red-cell turnover was detected by indirect measurement (hemoglobin, reticulocyte count, plasma hemoglobin, serum haptoglobin) or by turnover of 51Cr-labeled erythrocytes. Our findings fail to support the likelihood of a significant erythrocyte membrane defect.


Western equine encephalitis with rapid onset of parkinsonism. A patient with confirmed western equine encephalitis had the rapid onset of postencephalitic parkinsonian sequelae. This observation corroborates similar previous but rare reports. Response to therapy with levodopa, dopa decarboxylase inhibitor, and trihexyphenidyl was dramatic. However, remission maintained for 12 months without medication suggests that the parkinsonism would have remitted spontaneously. In either case, this has not previously been reported with the western equine togavirus.


CT scan in inter hemispheric subdural hematoma. Clinical and pathological correlation. In a patient with a large interhemispheric subdural hematoma, the CT scan showed a radiodensity with a straight sagittal and a convex lateral border. This image correlated with the autopsy findings. It differs from CT scan images of intracerebral hematomas.


Ventilatory response in myotonic dystrophy. Patients with myotonic dystrophy often develop respiratory failure caused by alveolar hypoventilation. Abnormalities in the ventilatory response to hypoxia and hypercapnia may explain this phenomenon. Accordingly, hypoxic and hypercapnic responses were measured in seven patients with myotonic dystrophy who had only mild respiratory muscle weakness. Hypoxic response was significantly reduced, while hypercapnic response was affected more irregularly. It is possible that the high incidence of respiratory failure in such patients is related to decreased hypoxic ventilatory response, occurring because of an underlying neurogenic deficit.


Electro-oculographic analysis of opsoclonus: its relationship to saccadic and nonsaccadic eye movements. Opsoclonus and intention tremors of the body were seen transiently in a 20-year-old woman with postinfectious encephalopathy. Electro-oculograms showed multidirectional eye oscillations: oblique and disconjugate involuntary movements were commonly seen. The opsoclonus was triggered by both saccadic and nonsaccadic eye movements including those not concerned with visual fixation.


Experiences with a new ergoline (CF 25-397) in parkinsonism. Studies on rats with unilateral nigral lesions suggest that a new ergoline, CF 25-397, is a dopaminergic agonist that might improve parkinsonism. CF 25-397 induces less stereotyped behavior than other dopaminergic agents in rats, and might therefore cause less dyskinesia than levodopa in man. We investigated the clinical actions of CF 25-397 in nine patients. During treatment, severe deterioration resulted in hypokinesia and rigidity; five patients showed marked dysphagia and dysphonia. There was statistically significant deterioration in four timed tests. Mild improvement, not statistically significant, was noted in tremor. These results indicate that clinical implication of the response to potential therapeutic agents in rodent models of parkinsonism must be interpreted with caution.


Language, praxis, and the right hemisphere: clues to some mechanisms of consciousness. The linguistic capacity of each separate cerebral hemisphere was examined in a 15-year-old, callosally sectioned, normally right-handed male. The results demonstrated that while the right hemisphere was not capable of expressive speech, it could comprehend nouns and verbs, and also possessed the motor engrams necessary to carry out verbal and pictorial commands. In addition, the mute hemisphere was found to be capable of spelling the names of visually presented items by arranging letters as well as by writing with the left hand. Finally, the manner in which the left hemisphere dealt with the overt bodily response to commands presented to the right hemisphere suggested clues to what we feel are mechanisms by which a personal sense of conscious reality is created in the normal brain.


Neuro-Behcet disease: two cases and neuroradiologic findings. In two cases of Behcet disease, neurologic disorders antedated the characteristic ocular or mucocutaneous lesions by 6 to 32 weeks. Neuroradiologic investigation demonstrated expanding avascular foci in the basal ganglia, probably representing areas of infarction secondary to vasculitis.


Corneal reflex latency in trigeminal nerve lesions. Using a new technique to record the latency of the corneal reflex, we established normal values in 32 normal subjects between 10 and 80 years of age, divided into three age groups. In 15 patients with unilateral trigeminal nerve lesions, comparison of the responses on both sides permitted differentiation of afferent and efferent lesions.


Fibromuscular dysplasia of intracranial arteries in a patient with multiple enchondromas (Ollier disease). In a 32-year-old woman, cerebral angiography showed fibromuscular dysplasia of the left anterior and middle cerebral arteries. She had had epilepsy and subarachnoid hemorrhages with subsequent hemiplegia. Since she also had multiple enchondromas (Ollier disease), this may represent a case of Maffucci syndrome.


Cortical deafness: demonstration of the pathologic anatomy by CT scan. A 27-year-old man with a prosthetic mitral valve had bilateral cerebral infarcts that caused a nonfluent aphasia, oral apraxia, and deafness. A computer-assisted tomographic scan showed symmetrical bilateral temporoparietal lesions. A review of the literature on other cases of cortical auditory deficits suggests that the clinical syndrome of pure word deafness in many cases is probably a less severe form of cortical deafness and is due to less extensive bilateral temporal gray matter lesions. However, strictly white matter lesions may produce some cases of either syndrome.


The central nervous system in a case of neurolathyrism. We examined the central nervous system of a 67-year-old man who showed symptoms of lathyrism after being imprisoned at age 35 and fed on a diet of chick peas, Lathyrus sativus. The most obvious changes were loss of axons and myelin in the pyramidal tract in the lumbar spinal cord. These alterations correlated with spastic paraparesis. Other changes at the same level were a mild degree of degeneration of anterior horn cells. Pallor of Goll's tracts and axonal swelling in Goll's nuclei were also observed. A few examples of senile plaques and neurofibrillary tangles were observed in Ammon's horn.


Myasthenia gravis and Schmidt syndrome.

Myasthenia gravis and Schmidt syndrome. A patient with myasthenia gravis developed both Addison disease and primary hypothyroidism, with demonstrable anti-adrenal and antithyroid antibodies in her serum. The association of myasthenia gravis with Schmidt syndrome does not seem to be a chance occurrence, considering the autoimmune pathogenesis of each of these disorders.


Second trimester placenta previa. An apparently normal placentation. Placental positions in 214 patients scheduled for genetic amniocentesis were reviewed. Forty-five percent were found to have ultrasound findings of a low-lying placenta or partial or total placenta previa. At term, none of these patients required intervention for bleeding or placenta previa. Parity of 4 or more, but not age, correlated with an increased incidence of partial or total placenta previa, but not with a low-lying placenta. Low-lying placenta or placenta previa may be a normal variant in early pregnancy, suggesting that failure of the placenta to relinquish this position can result in an abnormally implanted placenta at term.


[Emergency surgery problems: selective decompression of esophagogastric varices]. The physiopathological premisses of portosystemic anastomosis and the modern approach to this question are explained. Stress is laid on the general and haemodynamic advantages offered by selective shunts and 6 cases (4 distal splenorenal and 2 coronarocava shunts) are presented. As yet the postoperative period is not long enough to enable a final judgement to be formed. However, it can be stated that the success of this form of management is primarily determined by the proper choice of candidates, i.e. patients with non-decompensated initial cirrhosis and unimpaired portal hepatic flow.


The rights of the employee as patient.

The rights of the employee as patient. In summary, the rights of the employee as patient are essentially the same as they would be in any health care practitioner-patient relationship, whenever diagnosis and treatment are contemplated. When they are not involved and job fitness alone is at issue, the employee or potential employee has the right to expect that the practitioner will exercise reasonable skill and care in making the medical decision of employability. He probably also has the right to expect that any personal medical information generated in the course of making the decision remain confidential, unless it bears directly on his ability to do the job.


Peer review: accountability and legal implications. In summary, increasingly accountability implies the acceptance of peer review as an integral part of nursing practice. Peer review in turn presents a variety of ethical and legal implications and, beyond the scope of this discussion, educational, organizational and psychological implications as well. We must proceed to identify the components necessary to a sound peer review approach, starting with ethical issue and the necessary data base to measure outcomes of care right through the mechanisms of process and structural arrangements. Then, of course, we must ensure the application of rigorous examination of results. Whether QAPs as a whole or peer review as a specific, results are not dependent solely on the provider and client. Environmental and organizational factors must also be considered. Outcomes of care and performance are a function of the interaction of a variety of factors. In essence, the onus for assuring that the profession will meet its public accountability rests largely with each and every one of us. Every professional practitioner in the context of peer review will be compelled to be involved in assessment of the many dimensions of patient care. If professional self-requlation is to be effective, all professional nurses must be involved. In sum, we are the ones who must determine the what and the how of accountability for the nursing profession.


Postganglionic sympathetic activity with correlation to heart rhythm and central cortical rhythms. 1. Renal sympathetic nerve activity, ECG and parieto-occipital EEG were recorded in dogs anaesthetized with chloralose. The carotid sinus nerves were cut. Autocovariance functions and power spectra for these variables were computed. 2. During cooling of the vagus nerves, the integrated renal sympathetic activity exhibited rhythms which were correlated to the delta-theta rhythm of the EEG. This rhythm was also present with the vagus nerves functionally intact, but a cardiac rhythm was dominant. 3. Blood pressure-dependent neurones in the lower brain stem reticular formation have both cardiac and central cortical rhythms. This provides a hint that these neurones might be involved in the sympathetic tone generating network.


Control of the internal anal sphincter (manometric study with human subjects). Manometric studies of the functions of the internal anal sphincter were performed in 73 children. Twenty-one out of these patients were normal subjects, the others had neurological lesions either central (sacral meningocele, dorsal cord transections) or peripheral (Hirschsprung's disease). It was found that the functions of the internal sphincter do not depend upon mechanical factors and are subject to nervous control. The pathways for control of the basal sphincteric tone are discussed. It is likely that this tone depends not only of the sympathetic pathways, but also of fibers of the sacral outflow. It is shown that the recto-anal inhibitory reflex is specific and independent of peristalsis. Arguments are given to prove that this reflex is an activity proper to the intra-mural plexus which is, however, subject to and regulated by the sacral cord.


Modulation of hindlimb reflexes by tonic neck positions in cats. In cats with the head fixed in a stereotactic frame a slight facilitation of the gastrocnemiussoleus (GS) monosynaptic reflexes in the ipsilateral hindlimb, accompanied by an inhibition of the monosynaptic reflex of the antagnostic deep peroneal nerve (DP), occurs following lateral flexion and rotation around the body axis at an angle of 25 degrees. Following dorsiflexion of the body a moderate inhibition of the extensor reflexes (GS) up to 10% and a reciprocal slight excitation in the same range of the monosynaptic reflexes of flexor muscles (DP) is recorded. On the other hand after ventriflexion of the body there is a marked inhibition in the range of 50% of all the monosynaptic extensor and flexor reflexes for all the stimulus intensities used. This inhibition can already be demonstrated in ventriflexion of 10 degrees-15 degrees. All these patterns of reflex modulation are similar in cortically intact, ischemically decorticated and precollicularly decerebrated cats. Furthermore these patterns are not essentially influenced by cerebellectomy, although all reflex amplitudes are reduced. However, the spinal reflexes can not be modulated by these body movements after acute spinalization.


Physiological responses of dogs on exposure to hot, arid conditions. Acid-base status. Acid-base parameters were determined in chronically cannulated dogs exposed to ambient temperatures increasing from 25-47 degrees C (with relative humidity below 30%). pH increased from 7.409 +/- 0.004 (S.E.M.) to 7.538 +/- 0.017, PaCO2 decreased from 33.0 +/- 0.5 to 20.9 +/- 1.2 torr, and [HCO3-] decreased from 20.9 +/- 0.3 to 17.2 +/- 0.4 mEq/l. Minimal base excess change, together with a rapid return to normal parameters upon recooling to 25 degrees C, suggests that the stress is almost exclusively respiratory, with little metabolic involvement. Analysis of serial exposures shows no acclimatization effect in acid-base status. This suggests the possible existence of natural acclimation to heat in dogs maintained in a warm climate, permitting excellent tolerance of hot, arid conditions with limited acid-base disturbance.


Physiological responses of dogs on exposure to hot, arid conditions. Serum constituents. Serum electrolytes, metabolites and enzymes were determined in arterial blood of chronically cannulated dogs at room temperature and on exposure to 44-50 degrees C. These dogs were naturally acclimated to hot, arid conditions. In dogs maintaining their rectal temperatures (TR) below 40 degrees C, no significant changes were seen in the levels of Na+, Cl-, cholesterol, uric acid, alkaline phosphatase, lactic dehydrogenase or glutamic-pyruvic transaminase (SGPT). K+, CO2, glucose decreased significantly, and urea nitrogen (BUN) and glutamic-oxaloacetic transaminase (SGOT) showed small but significant increases. In several cases of excitable dogs, in which TR increased above 40 degrees C, we found large, significant increases in uric acid, SGPT and SGOT, and a decrease in cholesterol. The results suggest that in dogs maintaining their TR when exposed to high temperatures, changes in serum constituents indicate merely the presence of respiratory alkalosis and an increased energetic demand. When control of TR is lost, changes occur which suggest liver, and possibly cardiac, tissue damage.


Cardiac and respiratory rhythmicities in cutaneous and muscle vasoconstrictor neurones to the cat's hindlimb. Cardiac and respiratory rhythmicities have been investigated quantitatively in postganglionic vasoconstrictor neurones supplying skeletal muscle and skin of the hindlimb in chloralose anesthetized, immobilized cats. Both rhythmicities are largest in muscle vasoconstrictor neurones, smaller in vasoconstrictor neurones supplying hariy skin, and smallest in vasoconstrictor neurones supplying hairless skin. The magnitude of the cardiac rhythmicity in the vasoconstrictor neurones is positively correlated with the quantitative reaction to systemic hypoxia.


[Gelatin resorcin formaldehyde glue in vascular surgery (author's transl)]. The use of this biological glue appeared to us to be of particular interest in the surgical treatment of dissecting aneurysms of the aorta. An experimental investigation revealed its hemostatic and adhesive properties, and demonstrated its merit when used as material in vascular embolization.


Genes for gamma-globin in human adult erythroid DNA. Purified gamma-globin specific complementary DNA has been used to demonstrate the presence of the gene for gamma-globin in DNA from human adult red blood cells. This finding sheds doubt on any theory involving looping-out excision of genes to explain the switch over from synthesis of gamma-globin to beta-globin at birth.


Morphological functional changes of aortic endothelium during different types of hypertension. In rats, ligature of the aorta between the renal arteries produces hypertension which in the early phase (6-7 days) is associated with elevated plasma renin content, and later (40 days) is associated with low plasma renin. During the early phase, the endothelium of the aorta shows elevated permeability to HRP, endothelial cells are hypertrophic and contain bundles of actin microfilaments. During the late phase, permeability to HRP is normal, the endothelial cells are flat and do not contain bundles of microfilaments. Probably the endothelial cells of aorta react in different ways to various hypertensive stimuli and/or adapt to high levels of blood pressure.


[Reactivity of castrated female rats to estrone in alloxan diabetes]. After the administration of alloxan (in 23 days) to adult female rats the mass of the ovaries, the uterus, and the vagina altered, but the height of the uterine endometrium and the thickness of the vaginal epithelial layer decreased. When estron was administered to castrated rats with alloxan diabetes the mass of the uterus and the vagina and the height of the uterine endometrium, and of the epithelial layer of the vagina increased to the same degree as in rats with the intact pancreas; reactivity of diabetic rats to estrogen approached the sensitivity of animals with normal pancreatic functions.


Some aspects of aortic medial explant morphology and lipid metabolism. This study has examined some aspects of lipogenesis in both primary aortic medial explant cultures and intact aortic intima-media preparations. It has been shown that both lipogenesis and cellular ultrastructure vary with the age of the culture. Additionally, data has been presented indicating marked qualitative and quantitative differences in lipogenesis between explant cultures and intima-media incubations. Finally, it has been shown that the addition of either normo- or hyperlipemic human serum to explant cultures can significantly modify acetate incorporation into both triglycerides and cholesteryl esters and induce significant changes in ultrastructural morphology. it is concluded that one should interpret cell culture data with caution, particularly with respect to culture age, serum composition, and ultrastructural cellular viability.


Ca and Na spikes in egg cell membrane.

Ca and Na spikes in egg cell membrane. Na, Ca and K channels of the cell membrane are distinguished by different kinetics of their currents, by their different ion selectivities, and by their different sensitivities to different pharmacological agents. These channels are already found in unfertilized egg cells in many animals. Elimination of existing channels and creation of new channels occur during the process of differentiation.


Ultrastructure of meiosis and polar body formation in the egg of the mud snail, Ilyanassa obsoleta. Ilyanassa eggs pinch off two polar bodies at the animal pole prior to the first cell division. During prophase and early metaphase the meiotic apparatus is separated from the cortex by a layer of lipid droplets and can be found in any orientation relative to the cell surface. As the astral microtubules grow, the meiotic apparatus appears to be pushed toward the animal cell surface by the expanding internal aster. By late metaphase and early anaphase the peripheral aster first flattens and then appears to be folded down around the spindle, as the peripheral half-spindle is extruded out of the egg. The internal aster and the spindle remain intact at telophase, while the peripheral aster disassembles. A cortical fine filamentous layer is present after early metaphase into which peripheral aster microtubules enmesh. Such an interaction may provide the means of attachment of the meiotic apparatus to the cell cortex. A band of microfilaments is organized around the neck of the forming polar body, and it is thought to act as a contractile ring. It is unlikely that the stimulus for meiotic cytokinesis is like that established for mitotic cytokinesis in eggs; i.e., the region of astral overlap during metaphase determines the site of the furrow, since during meiotic metaphase the region of astral overlap is in the cytoplasm well below the surface. The cleavage furrow does bisect the spindle.


5,7-Dihydroxytryptamine lesions of the ascending 5-hydroxytryptamine pathways: habituation, motor activity and agonistic behavior. Rats were injected stereotactically in mesencephalon with 5,7-dihydroxytryptamine (5,7-DHT) in the medial 5-hydroxytryptamine (5-HT) pathway (n = 8) and in the medial plus the lateral 5-HT pathways (n = 7) or injected with vehicle (n = 8), or sham-operated (n = 8). The 5,7-DHT lesions reduced the in vitro 3H-5-HT uptake in the hypothalamus and the cortex cerebri to 27-51% of control values, 3H-noradrenaline uptake was not significantly changed. 5,7-DHT lesions of the medial, and of the medial plus the lateral, 5-HT induced mouse killing behavior and increased number of boxing positions in the shock elicited fighting test. Both lesions also reduced the rate of habituation to touch, but only the lesion of the medial plus the lateral 5-HT pathway significantly reduced the rate of habituation to acoustic stimulation. Activity in the home cage was not significantly changed by the lesions. It was concluded that selective chemical lesions of the ascending 5-HT pathways result in prolonged habituation of the orienting response and increase in particular components of agonistic behavior. The increase in locomotor activity observed after electrolytic lesions of nucleus raphe medianus seems not to be due only to lesion of the 5-HT neurons ascending from this nucleus.


Tolerance and dependence after chronic administration of clonidine to the rat. Acute administration of clonidine (10-70 microgram/kg, IP) disrupted operant behavior maintained by a fixed ratio schedule of reinforcement [1]. When chronically administered (100 microgram/kg, IP and 3 microgram/ml in drinking water) tolerance to the behavioral depressant effect developed within a few days and was complete by 14 days. Abrupt termination of drug treatment in tolerant rats resulted in an abstinence reaction which was characterized by suppression of operant performance for as long as one week. These results demonstrated the development of tolerance to and dependence on clonidine in rats. These behavioral observations in rats may be related to rebound hypertension and irritability of patients given this alpha-adrenergic agonist for treatment of hypertension.


Fatty acid and tryptophan changes on disturbing groups of rats and caging them singly. The effects of disturbing groups of 24 hr fasted rats on plasma unesterified fatty acid (UFA) and tryptophan concentrations and brain tryptophan concentrations were investigated. Removing rats from cages rapidly increased plasma UFA and corticosterone and decreased plasma and whole blood tryptophan of cage mates. The disturbance also appeared to influence biochemical values of rats in other cages within the same chamber. Effects specific to individual cages were also suggested. In subsequent experiments 24 fasting rats caged together were rapidly transferred to 24 separate cages and killed at intervals. Plasma UFA rose to a maximum by 12 min and then fell toward initial values. Plasma total tryptophan concurrently fell then rose. Its percentage in the free (ultrafilterable) state, and in some experiments the absolute values of free tryptophan rose then fell. When the latter rise was marked then brain tryptophan and the 5-HT metabolite 5-hydroxyindoleacetic acid rose. Tyrosine changes were negligible. Thus altered brain tryptophan level and 5-HT metabolism may be associated with plasma tryptophan changes caused by brief environmental disturbance.


Reversal of cycloheximide-induced amnesia by adrenergic receptor stimulation. Amnesia for a multiple trial appetitive spatial dicrimination habit induced by the protein synthesis inhibitor cycloheximide (CXM) was reversed by peripheral injections of both alpha (clonidine) and beta (isoproterenol) norepinephrine receptor stimulators. Stimulation of dopamine receptors with piribedil and acetylcholine receptors with pilocarpine was ineffective in reversing amnesia. The clonidine-induced recovery was blocked by phentolamine and the isoproterenol recovery by propranolol. Examination of the temporal parameters of clonidine-induced recovery indicated that the amnesia was prevented if the agonist was injected either before training and CXM treatment, up to 1 hr after training and up to 3 hr prior to testing. Clonidine also alleviated amnesia induced by another protein synthesis inhibitor anisomycin, for a shock motivated brightness discrimination habit. These data suggest that the transient amnesia induced by CXM may be a consequence of disruption of adrenergic mechanisms and more specifically that norepinephrine may play an important role in memory retrieval.


Behavioral effects of neuroleptics, apomorphine and amphetamine after bilateral lesion of the locus coeruleus in rats. Bilateral lesions of the locus coeruleus (LC) markedly increased susceptibility to the cateleptogenic effects of neuroleptics. The apomorphine-induced stereotypy was enhanced in rats with lesioned LC whilst amphetamine stereotypy was only slightly increased. No changes in locomotor activity have been observed in LC-lesioned rats treated with apomorphine and amphetamine. This data indicates that lesions of the LC produce decreased activity of dopaminergic brain neurons as well as supersensitivity of dopaminergic receptors.


Barrel rotation induced by vasopressin and related peptides in rats. Intraventricular injection of arginine-8-vasopressin and its analogues vasotocin and lysine-8-vasopressin into rat brain evoked a special rotational behavior resembling somatostatin-induced barrel rotation [1]. Oxytocin and oxypressin were less active while vasopressin fragments had no effect. Vasopressin-induced barrel rotation was accompanied by pathological symptoms indicating a disturbance of muscle tone regulation and is considered to be a non-specific and toxic effect. This rotational behavior was not prevented by atropine, propranolol, phentolamine, methylsergide or haloperidol but was reduced by chlorpromazine, probably due to the latter's muscle relaxing activity.


Alkali metal cations: effects on isolation-induced aggression in the mouse. Alkali metal cations were given in varying doses over 14 days to CF-1, male mice, isolated for 4 weeks prior to testing for isolation-induced fighting. Lithium and cesium in doses of 4.5 and 6.0 meq/kg reduced the duration of isolation-induced aggression in a 15 min test period when compared with controls. Toxicity was evident in the cesium-treated, but not the lithium-treated mice. No enhancement of aggression was seen in the rubidium-treated group.


[Review of the effects of cytochalasin B on cell division, cell movemnts, cell exchanges, and cell morphology]. Cytochalasin B is known to induce quiescence in cells. We present here a comprehensive view dealing with the effects of cytochalasin B on cell division, movements, exchanges and morphology. The controversial mode of action of the drug is also considered.


Further observations on functional deletion of paraocular glands in the fowl (Gallus domesticus). Methods are described for inoculation of the fowl Harderian gland with sclerosing reagent and for its surgical excision. Though very destructive the former procedure proved the less reliable in achieving the gland's complete functional deletion. Three weeks after surgical removal of the Harderian glands the lachrymal glands of 10-week-old fowls were heavier and contained more immunocompetent cells than the glands of intact and sham operated birds. When adult birds deprived of both paraocular glands were given sheep erythrocytes or Newcastle disease virus by eye drop they developed slightly higher than normal titres of serum antibody but failed to produce lachrymal antibody.


[Clinical demonstrations: 1. Renal tubular acidosis; 2. Breast neoplasm with bone metastases in a man; 3. Thrombotic thrombocytopenic purpura; 4. Precocious eclampsia in a 22 years old primipara]. Four patients with metabolic and/or circulatory failure are described. The clinical, biological and radiologic features of reversible renal failure with renal tubular acidosis are presented. A case of male breast carcinoma is discussed with reference to the effect of steroids on the hormonal receptors at the malignant cell. The pathophysiology of thrombotic thrombocytopenic purpura and its treatment (splenectomy and platelet aggregation inhibitors) are discussed. An overview of eclampsia and its relationship to underlying kidney disease is presented. It may be concluded that a metabolic and/or ischemic imbalance interferes with the normal activity of the reticular substance and the brainstem, which normally maintain the state of consciousness.


[Sleep organisation in the kitten following early lesions to the structures involved in the regulation of alertness (author's transl)]. Electrolytic lesions were used to destroy the anterior raphe nuclei and the loci caeruleus and subcaeruleus in kittens at varying stages of maturation. No lesion performed in the first week after birth led to changes in the characteristics of sleep in the 8 post-operative days. On the other hand, lesions performed in kittens aged 1-2 months had the same effect as is described as occurring in the adult animal. The structures which are responsible for states of alertness thus appear to start acting during the post-natal period.


Melatonin induction of gonadal quiescence in pinealectomized Syrian hamsters. Pinealectomized Syrian hamsters were injected thrice daily with 25 micrograms of melatonin per injection. The injections were administered at 3-hour intervals either during the day or during the night of a photoperiodic cycle of 14 hours of light and 10 hours of darkness. After 6 weeks of treatment with melatonin during the night, both pinealectomized and intact hamsters had reduced testis weight, and pinealectomized hamsters showed decreased levels of serum gonadotropins. Injection of melatonin during the day for 7 weeks either once (75 micrograms) a day or thrice (25 micrograms per injection) daily caused a reduction in testis weight in pinealectomized hamsters. Both pinealectomized and intact females injected with melatonin thrice daily during the day became anovulatory by week 7 of treatment. These results are similar to those observed when hamsters are exposed to a short photoperiod, suggesting that melatonin may be acting as a hormone in mediating the effects of photoperiod on the reproductive system of the Syrian hamster.


Histone occurrence in chromatin from Peridinium balticum, a binucleate dinoflagellate. Peridinium balticum is one of two dinoflagellates known to have dissimilar nuclei together in the same cell. One nucleus (dinokaryotic) has permanently condensed chromosomes, while the other (eukaryotic) does not have morphologically distinct chromosomes. Acid extracts of chromatin prepared from a mixture of dinokaryotic and eukaryotic nuclei and purified eukaryotic nuclei give four bands that co-migrate with four of the five histones from calf thymus when analyzed in urea-containing polyacrylamide gels.


Anencephaly with incomplete twinning (diprosopus). A case of diprosopus with anencephaly is presented. It is suggested that such concurrence of neural tube defects and incomplete twinning corroborates the notion that a single pathogenetic mechanism may be common to both neural tube defects and monozygotic twinning.


A comparison of the distribution, metabolism and excretion of ethylenethiourea in the pregnant mouse and rat. Pregnant mice and rats were treated by stomach intubation on day 15 g of gestation with 240 mg/kg of ethylenethiourea (ETU) made up in part with radiolabeled ETU. Animals were sacrificed at specific times post-treatment, and maternal tissues, fetus, urine and feces were collected for determination of radioactivity. Maternal and fetal tissue levels of ETU were similar at three hours post treatment; thereafter, the mouse (maternal and fetus) showed much less ETU than the rat. The t1/2 of ETU elimination from the maternal blood was 9.4 and 5.5 hours for the rat and mouse, respectively. Analysis of urine by thin-layer chromatography and radiochromatography revealed that the mouse and rat metabolized ETU by different pathways. Furthermore, the mouse is able to metabolize ETU to a greater extent than the rat.


Bovine like-sexed male twins with chromosomal chimerism. Sex-chromosomal chimerism (XX/XY) was described in a pair of bovine like-sexed male twins. One of the twins showed malformed external genitalia, and the other was phenotypically normal. It is proposed that sex-chromosomal chimerism is associated with a choriovascular anastomosis between like-sexed twins examined and "female fetus" which may be overlooked at parturition.


Physiological patterns in early morning asthma. Sixteen asthmatic patients who regularly showed early morning falls in peak expiratory flow rate of more than 25% were studied at 06.00 hours and 14.00 hours. At 06.00 hours considerable deterioration in static lung volumes and airways resistance occurred which was typical of an attack of acute asthma. Blood gas analysis showed less variation, with mild hypoxia and a wide alveolar-arterial oxygen tension gradient throughout the study. Diurnal variation was also seen in the response of maximal mid expiratory flow rates to the inhalation of helium/oxygen mixture in seven patients, but another seven were consistently non-responders. The overall pattern of results suggests that the calibre of both large and small airways decreased at night but improvement was more complete in large airways during the day. Despite these findings patients had few symptoms at 06.00 hours when most of them showed marked reversibility after inhaling aerosol salbutamol. This phenomenon might account for the sudden nature of some asthma deaths as these often occur in the early morning. If this is the case, the minority of patients whose early morning decline in lung function was not immediately reversible would appear to be at greatest risk.


[Properties of cells determining their immunosensitivity]. Three cell cultures of C3H mouse strain--L, MMT1 and embryonic fibroblasts have different sensitivity to cytolytic action of allogeneic antiserum and to immune lymphocyte action. A sensitivity to cytolytic action of antiserum for these cultures is correlated with the amount of corresponding transplantation antigens. A sensitivity to cytolytic action of immune allogeneic lymphocytes is correlated with the amount of corresponding antigens as well as cell with properties determining the level of lymphocyte sorption on them.


[Replication of chromosomal DNA in Chinese hamster cells, cultivated at different temperatures]. DNA fiber autoradiography was used to measure the rate of replication and the size of replication units in Chinese hamster cells cultured at 34, 37 and 39 degrees C. The average rate of DNA replication per single fork is 0.6 mcm/min at 34 degrees and 0.8 mcm/min at 37 and 39 degrees. In contrast to the change in the rate of DNA replication, no change was found in the size of replication units, which are more than 200 mcm at 31, 37 and 39 degrees. The change of the length of the S phase at various temperatures is determined only by the rate of DNA replication.


[Serum alkaline phosphatase and transaminase activity in cows with clinical ketosis]. Studied was the activity of the alkaline phosphatase and the transaminases in the serum of clinically normal and ketosis-affected cows as well as cows with an affected liver. The changes were followed in the activity of SGOT and SGPT that had set in as a result of five-day starving and the injection of prednisolon at the rate of 0.5 mg/kg in the course of four days. The activity of serum alkaline phosphatase in ketosis-affected cows proved unchanged--1.99 +/1 0.35 VE. A moderate increase was established in cows with liver affections-- 3.73 +/- 0.35 VE. The hyperenzymemia in ketosis-affected animals, in animals with liver injuries as well as in cows subjected to starving or prednisolon treatment was substantiated by the higher activity of SGOT. Dependable higher values of SGOT were established in the serum of freshly calved cows as compared to cows of advanced lactation. The higher activity of SGOT in animals with ketosis is associated with the physiologic status, starving, enzyme induction, inadequacy of the cell energy balance--all limiting the possibility of it being ascribed with preference to the tissue proliferation or destruction, which is to be expected with cows having an affected liver.


[Relationship between the sensitivity of the delayed agglutination test and synthetic detergents]. Residual amounts of detergents (Losk, Bio-73, Alka-lux, Bourgas, Bourgaslux, and Vero) in a concentration of 10-5 to 10-7 in physiologic saline can inhibit the agglutination titers by 3 to 5 degrees. This could mislead in the assessment of the reaction with regard to its diagnostic value. It is admitted that the inhibition produced is due to changes in the antibodies--drop in the total protein and light variations in all protein fractions as well as in the probable surface deterioration of the antigen, leading to its defective agglutinability. It is suggested to rinse more than five times all glassware that has been cleaned with detergents.


[Occurrence of nematodiasis among sheep and goats]. The spread was studied of sheep and goat nematodirosis in the conditions prevailing in the district of Varna for the 1973-1975 period. Füleborn's method was employed with a total of 24,909 coprosamples taken from the animals as follows: sheep--12,690, weaned lambs--7370, lambs--3355, kids--782, and goats--712. Partial helminthologic postmortem examinations were carried out by the digestive method of 104 sheep, 33 goats, 142 weaned lambs, 96 lambs, and 35 kids. The percent of infected animals was determined on the basis of the coprostudies as follows; lambs--20.9 (per cent), kids--26.6, weaned lambs--54.9, sheep--22.6, goats--24.8. The necroscopic investigations revealed 32.3, 40, 64.8, 32.5, 39.4 per cent, respectively. The average parasite burden in number of helminths was 313 (lambs), 85 (kids), 1517 (weaned lambs), 586 (sheep), and goats--290. Greatest number of Nematodirus parasites was found in weaned lambs--16,000, followed by sheep--2260, lambs--1840, goats--800, and kids--240. As many as 100 per cent Nematodirus carriers were found on a sheep farm in the district. It was also found that the north plains of the district are less infected as against the south parts embracing the northern slopes of the Balkan mountain, and along the course of the Kamchia river. It was found that sheep on the private farm-holdings were more strongly infected than those on the cooperative farms. On the other hand, it was established that on farms where lambs and weaned lambs graze together with the adults the former prove more strongly infected than those that are on isolated grasslands.


[Immunity after newcastle disease in man (author's transl)]. 5 patients, in whom Newcastle disease was diagnosed on the basis of a positive haemagglutination inhibition test (HIT) following contact with affected chickens in 1970 were re-examined recently. The ophthalmological findings were negative at follow-up in all cases. The HIT was still positive (1 : 8) in one patient only; no antibodies were detected in the other four patients.


[Comparative morphological studies of yeast cells using automated image analysis]. The ultrastructural alterations in cells of Candida utilis caused by the influence of copper ions have been studied by means of quantitative image analysis. A model has been proposed which presents the following informations: The main effect of the copper ions is represented by an increase of the volume of the whole cell and of that part of the cell which consists of nucleus, vacuoles, and inclusions (particles and globules). Nevertheless, neither the absolute volume of mitochondria, nor the density of mitochondria are influenced by high concentrations of copper ions in the culture medium.


[Effect of calcitonin on various stress ulcer models in the rat]. The preventive effect of calcitonin (10 microgram/kg subcutaneously) on stress ulcer was examined in 135 rats. Three stress models were used: (1) immobilization, (2) immobilization and ketamine, (3) hypovolemia. Significantly lowered lesion rates under calcitonin were found in immobilization stress and hypovolemia models (14%, resp. 16%), compared with controls (78%, resp. 56%). Reduction of the severe lesions of the mucous membrane was the only significantly different result in the combination of immobilization and ketamine.


Sexual behavior in the cockroach genera Periplaneta and Blatta. III. Aggression and sexual behavior. Relationships between aggression and sexual behavior were examined in 6 cockroach species (genera Periplaneta and Blatta) in a descriptive manner. Aggressive encounters were classified according to intensity level. The behaviors themselves were scrutinized for differences according to varying contexts such as food and water deprivation and sexual situations. Sexual and species differences in aggressive behaviors were analyzed. Sexual encounters in cockroaches are considered as conflict situations in which many of the sexual behaviors of male male serve to neutralize aggressive behaviors of the female. Major adaptations include a male pheromene, tergal glands and defensive manoeuvering with body and legs.


[Several mechanisms of acetylcholine participation in the processes of formation and fixation of temporary connections]. Microphoretic application of acetylcholine (ACH) to the neurones of the rabbit sensorimotor cortex elicits changes of spontaneous and evoked activity which do not correlate with one another and which persist up to two minutes after the end of ACH application. Following the formation of a defensive conditioned reflex to sound, the reactions to ACH in units involved in the formation of the temporary connection are intensified as compared with their reactions before conditioning and with reactions of those neurones which did not elaborate a temporary connection. The possible mechanisms of ACH participation in the processes of elaboration and fixation of temporary connections are discussed.


[Effect of destruction of midbrain raphe nuclei on the behavioral effects of dopaminergic substances and I-tryptophan]. In experiments on male albino rats, after electrolytic lesions of dorsal and median mesencephalic raphe nuclei, l-DOPA and apomorphine did not exert their stimulating effect on aggressiveness and emotional reactivity, seen in control false-operated animals. The inhibitory effect of haloperidol on emotional reactivity increased after raphectomy. The stereotype behaviour elicited by dopaminergic agents and their effects on exploratory motor activity were not changed by raphectomy. It is assumed that the effects of dopamino-potentiating agents on emotional reactivity are partly mediated by their influence on serotonergic system.


Ethological study of African carpenter bees of the genus Xylocopa (Hymenoptera, Anthophoridae). Over 200 bees of 4 African Xylocopa species were observed for 3 months on the island of Rubondo (L. Victoria, Tanzania). Some 40 burrows were investigated, 100 bees marked. Building techniques are minutely reported; burrow construction simplifies defence and allows re-use by succeeding generations. Food plants, collecting, provisioning and all aspects of ontogenesis are treated, insight given into pupal leg mobility and the much-debated emergence order after eclosion: the first-hatched bee, in the rearmost cell, prepares the way for siblings. Copulation and the copulatory hold are studied using tethered femalefemale, and illustrated. A few colonization experiments are described and s spectrogram of begging sounds given. Meeting of the generations, feeding of the young and nest-defence by young siblings throw light on the evolution of primitively eusocial communities. The known literature is reviewed in each chapter.


Agonistic behaviour and dominance in stoats (Mustela erminea L.). Behavioural mechanisms underlying distribution in a stoat (Mustela erminea) population were examined experimentally in an enclosure simulating natural habitats with living prey. Agonistic behaviours, displayed by individual stoats of different sex and age while being tested in pairs in various combinations were recorded. Dominance relationships between different categories of stoats (femalefemale, adult and juvenile male male) were examined, as was social dominance between individual stoats of the same sex. In further experiments designed to examine the influence of establishment on the social dominance, the behaviour of established introduced animals was observed.


[Changes in the readiness for sexual and aggressive action of the sword tail Xiphophorus helleri (Pisces, Poeciliidae) under the influence of social isolation]. Social deprivation for 3 to 112 days caused an increase in copulation attempts and other motivational changes of sexual activities. Furthermore, it could be demonstrated that the tendencies for nipping, backing, copulation attempts, and gonopodial swinging fluctuated independently as a result of non-release of these activities. A rise in copulation tendency does not necessarily lead to increased aggressiveness. In the social hierarchy inferior individuals exhibited the highest levels of sexual and aggressive responsiveness.


[Polymorphism of Charcot-Marie-Tooth neural amyotrophy in uniovular twins]. The paper is concerned with a description of an atypical clinical picture of Charcot-Marie-Tooth neuronal amyotrophy in 2 eight-year old monozygotic twins. The traits of the disease were characterized by a deformation of the feet, mild cerebellar symptoms, development of ptosis and other myopathical symptoms, an enlargement of the transversal nerve of the neck and a distribution of the process to the proximal parts. The diagnosis was confirmed by EMG studies. The authors detected a large amount of abortive signs in many members of the family both along the mothers, as well as fathers line. This observation demonstrates clinical and genetical polymorphism of neuronal amyotraphy.


[Glucocorticoid function of the adrenals and the state of the sympathetic-adrenal system in newborn infants with an hypoxic lesion of the central nervous system]. The convened studies demonstrated an activation of the hypothalamo-hypophysis-adrenal and sympathico-adrenal system in the newborn with slight and moderately expressed forms of CNS lesions. At the same time there was a relative insufficiency of such functions in children with severe forms of CNS lesions. The achieved data permit to substantiate the pathogenetical therapy.


[Characteristics of the diurnal fluctuations of affect in depressions in adolescents with circular schizophrenia]. The paper is related to an analysis of the diurnal rhythm of mood in 87 adolescents suffering from circular schizophrenia. The diurnal mood fluctuations, as in adults, were seen in most of the patients where the depression had a tendency towards an increase and decrease, as well as in cyclothymo-like phasic depressive disorders. The author describes a special diurnal rhythm of mood in profound depressive states with agitation and hysterical components.


[Tuberous sclerosis in a premature infant (author's transl)]. A sporadic case of tuberous sclerosis in a stillborn infant is reported. The death at the 31st week of gestation was presumably due to the development of enormous rhabdomyomas of the heart. The typical cerebral lesions were fully developed as in patients decreased later in life. The atypical cells found in the cortical tubers demonstrated ultractructural features of reactive astrocytes. Moreover, they showed innumerable microvillilike projections on their surface and junctional complexes, mostly of the zonula adharens type, reminescent of ependymocytes. The significance of such glio-epithelial cellular features is discussed.