Thermal aspects of self-curing polymethylmethacrylate. Bone necrosis adjacent to self-curing polymethylmethacrylate is a matter of accepted fact. Among the possible causes are mechanical and vascular damage from the preparation of the bone cavity, chemical damage from the monomer and free radicals in the cement dough, and thermal damage from the heat of polymerisation, occurring in this order. Consideration of the tissue reaction to this material, theoretical calculation of the heat output from polymerising acrylic and interface temperature profiles, experimental observations of interface temperatures and maximal temperatures at polymerisation, together with clinical observations, all lead to the view that the bone necrosis is not a consequence of thermal damage, which is unlikely to be a cuase of failure of prosthetic fixation. Temperatures recorded from within polymerising acrylic masses are related primarily to the amount of monomer polymerising and are of no clinical significance in the fixation of prostheses. Interface temperatures are related primarily to the surface area of the interface and the thermal characteristics of the cooler material.


Radial nerve enclosed in the callus of a supracondylar fracture. At operation for the correction of cubitus varus by removal of a wedge based laterally, the radial nerve fortunately was first explored and found to run through a transverse bony tummel at the posterior level of a suprocondylar fracture sustained eight years previously. After elevation of the bony tunnel and nerve en bloc the osteotomy was completed; neurapraxia of the radial nerve soon recovered. With hindsight, the entrance and exit foramina of the tunnel could be clearly seen in the antero-posterior radiograph taken before the operation.


14C-glucose and 35S-metabolism of pig epiphysial cartilage and its variations after osteotomy. On in vitro incubation of articular and epiphysial cartilage of the ulna of the domestic pig 70 to 80 per cent of [U-14C] glucose was metabolised to 14C-lactate, but cartilage of the epiphysial plate produced up to five times as much 14C-CO2 as articular cartilage, and the specific radioactivity of 14C(or 35S)-chondroitin 4(6)-sulphate isolated from epiphysial cartilage (following 35S-sulphate incorporation) was about twice as high as that of articular cartilage. Six weeks after an osteotomy on both sides of the proximal epiphysial plate of the lift ulna, the glucose uptake, lactate production, and the specific radioactivity of the glycosaminoglycans displayed no significant differences when compared with those of the corresponding epiphysial plate of the control right ulna, whereas a 50 per cent increase in the oxidation of 14C-glucose to 14C-CO2 was observed.


Results of hypotonic duodenography in the diagnosis of pancreaticoduodenal cancer. Hypotonic duodenography has become a well accepted procedure for diagnosing pancreaticoduodenal cancer. In order to provide further data about the accuracy of this examination, 170 patients were reviewed who were suspected of having this disease and whose duodenogram diagnosis was later confirmed or disproved by surgery, autopsy, or clinical follow-up. The correct diagnosis was made in 158 patients (over-all accuracy of 93%). Of 31 ultimately proved pancreaticoduodenal cancers, 24 were correctly identified (specific accuracy of 77%). For 27 patients a positive diagnosis of pancreaticoduodenal cancer was made, and 24 of these later proved to have this disease (positive accuracy of 89%). For 110 patients, a negative diagnosis was made, and 103 of these proved to be free of pancreaticoduodenal cancer (negative accuracy of 94%).


Five years experience with the Björk-Shiley tilting disc valve in isolated aortic valvular disease. The non-overlapping tilting disc of the Björk-Shiley prosthesis has proved its efficiency in two respects: It provides an optimal orifice to tissue diameter ratio with a favourable rheology both at rest and during exercise even in the smaller valve sizes used for insertion in narrow aortic roots. It accounts for a minimal haemolysis because mechanical crushing of the red cells is minimized by such a non-overlapping closing mechanism. Regurgitation is negligible. The excellent durability of the prosthesis has been clinically testified. Thrombo-embolism has not so far occurred with effective Dicumarol treatment. Aortic valve replacement with the Björk-Shiley tilting disc valve is regarded as an elimination of the volume load of the left ventricle in AI and the pressure load in AS, thereby creating a pre-requisite for a normalization of the pump function of the heart.


Divalent ions and myocardial function during cardiopulmonary by-pass (CPB). Changes of total calcium, ionized calcium, and magnesium in plasma. In 17 patients who underwent openheart surgery with cardiopulmonary bypass using heparinized fresh blood for priming the heart-lung machine the following investigations were done: Blood samples taken at different periods of surgery were assayed for total calcium (Catot), ionized calcium (Ca++), magnesium (Mg), hemoglobin, total pasma proteins, and the acid-base-status. Considering the different kinds of cardiopulmonary bypass the patients were divided into three groups: In the first group the results ofsurgical procedure with and without hemodilution perfusion were compared. During hemodilution perfusion Catot decreased markedly whereas Ca++ remained nearly constant. In the second group the influence of different calcium concentrations of the prime solution on Catot and Ca was tested. A low calcium content of 2.8 mEq/1 lowered Catot and Ca++ to subnormal levels. In the third group results of Mg-induced cardioplegia were compared with findings during surgical procedure with anoxic cardiac arrest. A remarkable increase of magnesium at the perfusion onset could be observed. Magnesium remained within the upper level of normal range until surgery end and decreased to normal values in the postoperative stage. Since energy requirements of the arrested heart and thus the velocity of ATP-breakdown during ischemia are closely related to the Ca++ concentration of the extracellular space low plasma calcium levels are considered to be advantageous during cardiopulmonary bypass. Only at the end of partial bypass before the heart fully takes over circulating work a sufficient calcium substitution is recommended.


Induced ischemic cardiac arrest. Clinical and experimental results with magnesium-aspartate-procaine solution (Cardioplegin). The risk of open heart surgery can be lowered by combination of different methods of myocardial protection. 1. Cardioplegia with a potassium free Mg-1-aspartate and Procaine-solution (Cardioplegin). 2. Coronary perfusion after ischemia longer than 35-40 minutes in case of excessive left ventricular hypertrophy or failure. 3. Hypothermia. Surface cooling gives an additional safety if coronary perfusion is not ideal possible in case of multiple coronary stenoses. For patients with this dispositions a continuous coronary perfusion with cardioplegic solution might be advisable, as it was presented by Gercken in his paper. This method was used three times already in human, but is still in an experimental stage.


Anoxic cardiac arrest: Its effect on myocardial mitochondrial metabolism. Forty eight healthy dogs are subjects to cardiopulmonary bypass and divide into two groups according to whether the perfusion is performed in normothermia or moderate hypothermia (30 degrees C). The effects on oxidative phosphorylation and mitochondrial respiration, produced by increasing periods of myocardial anoxia from aortic cross clamping, are studied. A clear correlation is found between the disturbances of mitochondrial metabolism and the future cardiac recovery. Hypothermia shows a protective effect on the anoxic myocardium. It is concluded that the period of absolute safety in cardiac anoxia, as far as mitochondrial function is concerned, is 15 minutes in normal thermic perfusion and 90 minutes in moderately hypothermic ones.


What constitutes adequate perfusion?

What constitutes adequate perfusion? Success of a perfusion depends not as much on the perfusion system as how that system is applied. A bubble oxygenator system properly managed can be safely run for many hours. Perfusion rate alone is an inadequate criteria for conduct of an adequate perfusion. 75 to 100 cc/kilo/min. perfusion rate is recommended. Minimal mean arterial pressures (60-70 mmHg) are essential to maintain good function of all organ systems for many hours of perfusion and to avoid disseminated intravascular coagulation by keeping capillary beds open. Foreign blood (bank blood) should be avoided to a maximal degree as foreign blood along with hypotensive situations as with poor perfusions can increase predisposition to DIC and organ failure. Hypokalemia (plasma and intracellular) predisposes to poor peripheral resistance during perfusion and consequent low mean arterial pressures. Fifty to 150 meq. of potassium chloride injected into the perfusate is generally required. Excessively high pO2 (over 150) and/or excessively low pCO2 (under 35) may contribute to CNS aberrations. Cannula placement and size are important considerations as well as suction forces on the venous cannula due to hydrostatic pressure on the venous return line. This force must be varied at times for maximal return. The inferior canal catheter must be kept out of the hepatic veins.


Coronary circulation and myocardial oxygen consumption after extracorporeal circulation and after cardioplegic arrest. The influence of haemodilution or of blood perfusion on the coronary circulation, myocardial oxygen consumption and on haemodynamic parameters was examined in two comparable groups of patients with slight congenital acyanotic cardiac malformation without indications of myocardial damage. In a third group of patients similar data were collected before and after artificial cardiac arrest by cardioplegic solutions. No findings concerning this question could be found in the literature. Coronary circulation was measured by the argon-gas-technique of Bretschneider et al. A fall in haemoglobin of 4.8% caused by haemodilution resulted in the first group in an increase in the cardiac index of about 8% and an increase in the coronary circulation of about 100% over the initial value with a simultaneous decrease in the coronary resistance due to coronary dilatation and reduction in blood viscosity. The myocardial O2 consumption was raised in spite of an increase in the coronary-venous O2 saturation and a clear reduction in the AVD O2. In contrast, the patients in the blood perfusion group showed no change in the coronary circulation and coronary resistance although a decrease of the myocardial O2 consumption was observed. The cardiac index fell to about 16% in these patients. After cardioplegic cardiac arrest there was no significant reduction in myocardial blood flow except for a decrease of myocardial oxygen consumption of about 20% The results are discussed and cantra-indications of haemodilution perfusion and cardioplegic cardiac arrest mentioned.


Anomalous origin and distribution of coronary arteries. Review of 38 patients who underwent operation. Between October 1961 and December 1973, 38 patients with an anomaly in origin (15 patients) or distribution (23 patients) of the main coronary artery or one of its branches underwent operation at the Texas Heart Institute. The left coronary artery originating from the pulmonary artery occurred most frequently-in 13 of 15 patients. An aortocoronary artery bypass was performed in 12 patients with the saphenous vein used in ten of the 12, initially in 1965; and a Dacron tube graft in the other two. Of the 15 patients, only one died during the early period after operation. A follow-up of ten years revealed 11 asymptomatic patients; to date the longest period of patency of a saphenous vein graft is seven years in an 11-year-old girl. Of 23 patients with an unusual coronary artery distribution, 22 had tetralogy of Fallot, 20 of whom underwent total correction. In 21 of the 23 patients the left anterior descending coronary artery originated from the right coronary artery and crossed the right ventricular outflow tract. In two patients this abnormally distributed artery was injured through a vertical right ventriculotomy; both patients died from myocardial failure during the early postoperative period. Subsequently a transverse right ventriculotomy, either alone or combined with a right ventricular outflow and/or pulmonary artery patch enlargement was performed in 16 patients, and a double outlet right ventricle was created through insertion of a Dacron tube graft in two patients. With this method injury to the abnormal left anterior descending coronary artery was avoided and all 18 patients survived the operation. On the basis of our experience and today's advanced techniques, it is believed that most patients, including some under two years of age, can undergo correction of a left coronary artery originating from the pulmonary artery through insertion of a saphenous vein graft between the aorta and left coronary artery. During the surgical correction of cardiac anomalies necessitating a right ventriculotomy, a transverse or double incision in the right ventricular outflow tract in most patients will prevent injury to an abnormally distributed coronary artery branch; sometimes insertion of a Dacron tube graft between the right ventricular outflow tract and pulmonary artery is necessary.


Persistent atrio-ventricular canal. Case report of an unusual intermediate form. A case is described, which presented the following combination of anomalies: An interatrial and interventricular septal defect, absence of a cleft in the aortic leaflet of the mitral valve and in the septal leaflet of the tricuspid valve. The latter leaflet was underdeveloped and presented several perforations. On the bases of the characteristic electrocardiographic, angiocardiographic and anatomical findings this anomaly was considered to be an intermediate form of a common atrioventricular canal. Total repair was successfully performed utilizing the technique of Rastelli and McGoon.


Left atrial myxoma causing mitral insufficiency. Report of a case treated with mitral valve replacement. A case of left atrial myxoma causing mitral insufficiency treated with valve replacement and diagnosed preoperatively as mitral stenosis is presented. The presence of mitral regurgitation is very rare in atrial myxomas and requires mitral valve replacement. In our case mitral regurgitaion was due to the mechanical trauma of the valve by the tumor mass because the patient had no history of rheumatic fever and the pathologic examination of the valve displayed neither rheumatic nor myxomatous degeneration.


Revascularization of the superior mesenteric artery. From 1965 to 1973, 7 patients with severe chronic mesenteric vascular insufficiency have been successfully operated upon. Abdominal pain, weight loss and epigastric murmur were the most significant symptoms and signs in these diffusely atheromatous patients. Aortography with exposure in the lateral projection was essential for diagnosis and operative planning. Although two and often all three main splanchnic arteries were involved, revasculariztion of only the superior mesenteric artery restored normal hemodynamics. There was no operative mortality. Weight gain was dramatic and post-prandial pain disappeared in all patients. One patient diedone year and one half after the operation from an acute cerebro-vascular accidnet. Our surgical experience in this field, although small, is very gratifying and rewarding.


Renal function following aortic surgery. Following aortic surgery nearly two thirds of patients (65%) have demonstrable renal tubular damage, which is related to the disease process (aneurysmal disease being more commonly associated with renal damage than occlusive disease), the duration of aortic cross clamping and the volume of blood transfused. No relationship appears to exist between age, pre-operative hypertension, or chronic renal failure, and post operative renal tubular function.


Comparative analysis of prosthetic heart valve replacement on the basis of 300 cases. 1. The 224 patients have been followed-up for postoperative intervals of 1 to 8 years after operation, and the average duration of the follow-up has been 4 years. 2. Six types of heart valve prostheses have been utilized and evaluated with the longest (9 years) period of follow-up (a Starr-Edwards prothesis) and the shortest a Björk-Shiley valve. The best clinical hemodynamic characteristics, no thromboembolic complications were observed with the Lillehei-Kaster valve. Evaluation of longterm wear and thrombosis require further follow-up studies. 3. All patients, but 21, improved in functional capacity and are NYHA class I and II. Most of the patients had heart failure at the time of operation. These patients would have had a life expectancy of about two years from the onset of symptoms. Seventy two patients with heart failure as a prime symptom have thus outlived their anticipated life expectancy. 4. Systemic embolization was the most common postoperative complication in spite of the anticoagulant therapy.


Radiological study of the aetiological factors in venous obstruction of the upper limb. This study was undertaken in an attempt to define the exact relationship between intermittent venous obstruction of the upper limb and the acute condition of primary axillary-subclavian vein thrombosis. Three groups of patients were investigated, namely those with evidence of intermittent venous obstruction in one or both upper limbs, those with a previous history of an acute primary axillary-subclavian vein thrombosis in one upper limb and the control group of patients. The patients were investigated by subclavian venography with the limbs in both adduction and abduction. All patients with intermittent venous obstruction were shown to have bilateral evidence of venous compression in abduction in the region of the root of the neck and half of them showed a similar bilateral compression in adduction. All patients with a previous acute thrombotic state showed venographic evidence of post-thrombotic changes in the affected limb and in the unaffected limb all showed evidence of venous compression in abduction and half showed evidence of venous compression in adduction. The control group exhibited no evidence of venous compression in either adduction or abduction.


The injury potential of Fogarty balloon catheters. The problem of arterial injury following embolectomy using a balloon catheter is reviewed. Three illustrative clinical cases are presented. The cause of the arterial injury in these cases was due to rupture of the vessel. The hypotheses were that either the vessel was ruptured by direct penetration or bursting by overdistension of the balloon. The linear appearances of the arterial tear in two patients and a false aneurysm in the third suggested that bursting was the correct explanation. Laboratory investigation was carried out on animals and human cadavers and these confirmed that arterial injury and bursting can be caused by over-distension of the balloon catheter. If the maker's recommendations are strictly adhered to; that is, the balloon should not be over-inflated and the correct volume of fluid used then rupture of the artery is unlikely by occur.


An improved technique for below knee amputation. The author describes a technique for below knee amputation using a long and very broad posterior flap where the main vessels are divided and ligated distally. Two large "dog ears" at the end of the stump are de-epithelialized and buried providing extra padding. The technique conserves maximum blood supply and has proved to be safer than the methods described previously.


In vivo assembly of tight junctions in fetal rat liver. Examination of glutaraldehyde-fixed, freeze-fractured livers from 14-15-day rat fetuses provided the basis for the following observations. Membrane particles align in otherwise poorly particulated areas of the presumptive pericanalicular plasma membrane (A face), frequently forming a discontinuous "honey-comb" network joining small particle islands. Even at this early stage, contiguous B-fracture faces contain furrows, rather than rows of pits, distinguishing the linear particle aggregates on the A face as developing tight junctions rather than gap junctions. Short segments of these linear arrays merge with smooth ridges clearly identifiable as segments of discontinuous tight junctions. With the continuing confluence of particulate and smooth ridge segments, mature tight junctions become fully appreciable. We conclude that tight junctions form de novo by the alignment and fusion of separate particles into beaded ridges which, in turn, become confluent and are transformed into continuous smooth ones. At 21 days of fetal life, most of the images of assembly have disappeared, and the liver reveals well-formed bile canaliculi sealed by mature tight junctions.


Ten years' experience in heart valve replacement with artificial prostheses: immediate and long-term results in 1812 cases. A ten year follow up of 1,812 patients who underwent heart valve replacement with artificial prothesis between October 1963 and December 1973 is reported. Hospital mortality was 8.5% for aortic valve replacement; 13.6% for mitral valve replacement and 19.3% for multiple valve replacement. Late mortality was 13.3% for aortic valve replacement; 25.7% for mitral valve replacement and 25.9% for multiple valve replacement. The survival rate of the different groups of patients were actuarially determined. The survival rates following replacement of the aortic valves was 81% after 9 years. The main late complications are embolism and valve leakage. All surviving patients show a clinical improvement, particularly in the groups of isolated aortic a mitral valve replacement. The heart size reduction after operation has been measured. This is more evident 6 months after surgery: the average reduction was 23% after aortic valve replacement and 20% after mitral valve replacement.


Vena cava umbrella filter: experimental, hemodynamic and embolization studies. The Mobin-Uddin umbrella filter was placed into the inferior vena cava of mongrel dogs. Studies on hemodynamics and embolization were performed using blood flow and pressure determinations, cineangiography, and radiopaque emboli. Initially flow and pressure remained unaffected by the filter. At 70 minutes flow was only one fourth of the initial IVC flow. Immediately after positioning of the filter emboli up to 6 mm in diameter bypassed the filter, and smaller emboli passed through the perforations in the filter. Thrombus formation on the surface of the umbrella was already noted within one hour. At autopsy after two hours all the perforations were occluded by thrombus on both sides of the filter, and one week later the infrarenal vena cava was completely occluded by firm organized thrombus below and above the filter.


In vitro incorporation of (3H)threonine and (3H)glucose by the mucous and serous cells of the human bronchial submucosal gland. A quantitative electron microscope study. Incorporation of [3H]threonine and [3H]glucose by the mucous and serous cells of the human bronchial submucosal gland has been studied over 8 h using, for the first time in vitro pulse labeling and electron microscope autoradiography. In assessing the autoradiographs, two methods were compared, the circle analysis and the recently described hypothetical grain analysis. Preliminary studies showed formaldehyde to be the most suitable fixative. Chemical analysis of tissue revealed that [3H]threonine was incorporated into the polypeptide moiety of the bronchial gland product and that metabolites of [3H]-glucose were incorporated into the carbohydrate. Tritiated threonine was first localized in the endoplasmic reticulum of both mucous and serous cells and later migrated to the Golgi apparatus, while metabolites of [3H]glucose localized first mainly in the Golgi apparatus. From here, both radioactive precursors were next identified in vacuoles and, finally, in secretory granules. The mucous cell incorporated strikingly more of both radioactive precursors than the serous cell. Thus, it seems that oligosaccharides of mucous and serous cell glycoproteins are synthesized mainly in the Golgi apparatus and added there to the polypeptide core which is synthesized in the endoplasmic reticulum. The relationship of the mucous cell to the serous cell is discussed. It seems that under "normal" conditions each cell represents a different line but that injury may transform a serous cell into a mucous cell.


The effect of isolated perfusion on limb circulation in peripheral arterial occlusion. The effects of isolated limb perfusion were studied in 12 patients suffering from peripheral arterial occlusion. The hemodynamic investigations show that the vascular resistance decreases considerably during isolated limb perfusion. After administration of a vasodilator cocktail, added to the perfusion, the vascular resistance continues to decrease. Angiographic records, made before, during and after the perfusion, suggest that the calibre of the collateral vessels increases significantly and besides new collaterals can also be observed. The mechanism of the effects of isolated hyperbaric limb perfusion and its clinical value are discussed.


A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii. Sodium dodecyl sulfate gel electrophoresis of unheated, detergent-solubilized thylakoid membranes of Chlamydomonas reinhardtii gives two chlorophyll-protein complexes. Chlorophyll-protein complex I (CP I) is the blue-green in color and can be dissociated by heat into "free" chlorophyll and a constituent polypeptide (polypeptide 2; mol wt 66,000). Similar experiments with spinach and Chinese cabbage show that the higher plant CP I contains an equivalent polypeptide but of slightly lower molecular weight (64,000). Both polypeptide 2 and its counterpart in spinach are soluble in a 2:1 (vol/vol) mixture of chloroform-methanol. Chemical analysis reveals that C. reinhardtii CP I has a chlorophyll a to b weight ratio of about 5 and that it contains approximately 5% of the total chlorophyll and 8-9% of the total protein of the thylakoid membranes. Thus, it can be calculated that each constituent polypeptide chain is associated with eight to nine chlorophyll molecules. Attempts to measure the molecular weight of CP I by calibrated SDS gels were unsuccessul since the complex migrates anomalously in such gels. Two Mendelian mutants of C. reinhardtii, F1 and F14, which lack P700 but have normal photosystem I activity, do not contain CP I or the 66,000-dalton polypeptide in their thylakoid membranes. Our results suggest that CP I is essential for photosystem I reaction center activity and that P700 may be associated with the 66,000-dalton polypeptide.


The behavior of fibroblasts from the developing avian cornea. Morphology and movement in situ and in vitro. The early chick cornea is composed of an acellular collagenous stroma lined with an anterior epithelium and a posterior endothelium. At stage 27-28 of development (5 1/2 days), this stroma swells so that the cornea is 75-120 mum thick. At the same time, fibroblasts that originate from the neural crest begin to invade this stroma. Using Nomarski light microscopy, we have compared the behavior of moving cells in isolated corneas with the migratory activities of the same cells in artificial collagen lattices and on glass. In situ, fibroblasts have cyclindrical bodies from which extend several thick pseudopodia and/or finer filopodia. Movement is accompanied by activity in these cytoplasmic processes. The flat ruffling lamelli-podia that characterize these cells on glass are not seen in situ, but the general mechanism of cell movement seems to be the same as that observed in vitro: either gross contraction or recoil of the cell body (now pear shaped) into the forward cell process, or more subtle "flowing" of cytoplasm into the forward cell process without immediate loss of the trailing cell process. We filmed collisions between cells in situ and in three-dimensional collagen lattices. These fibroblasts show, in their pair-wise collisions, the classical contact inhibition of movement (CIM) exhibited in vitro even though they lack ruffled borders. On glass these cells multi-layer, showing that, while CIM affects cell movement, fibroblasts can use one another as a substratum. Postmitotic cells show CIM in moving away from each other. Interestingly, dividing cells in situ do not exhibit surface blebbing, but do extend filopodia at telophase. The role of CIM in controlling cell movement in vivo and in vitro is stressed in the discussion.


Mobility of normal and virus-transformed cells in cellular aggregates. The mobility of embryonic chick cells and cells of four established cell lines was examined in cellular aggregates. This was done by preparing aggregates of unlabeled cells and allowing cells of the same type, but prelabeled with [3H]thymidine, to adhere to the surface of the aggregates. After 2-1/2 days in agitated liquid culture the positions of the labeled cells within the aggregates were determined by autoradiographic techniques. Since the labeled and unlabeled cells were otherwise identical, the degree of penetration of the labeled cells into the aggregates was taken as a measure of the mixing or mobility of cells in the aggregate. With this procedure, embryonic chick liver, heart, and neural retina cells were found to move an average of 2.12, 2.68, and 4.00 cell diameters inward, respectively. Mouse fibroblast BALB/c 3T3 cells moved an average of 1.13 cell diameters inward, while Simian virus 40 (SV40)-transformed BALB/c 3T3 cells moved as much as 8.80 cell diameters inward, indicating that cells of the malignant SV40-transformed line were considerably more mobile than the corresponding nonmalignant 3T3 cells. In contrast, cells of the hamster fibroblast line NIL B moved 4.17 cell diameters in 2-1/2 days, while SV40-transformed NIL B cells moved 3.00 cell diameters in the same time. It was therefore concluded that infection with oncogenic viruses does not necessarily result in increased cellular mobility.


A stereological study of the glomerular filter in the rat. Morphometry of the slit diaphragm and basement membrane. Kidney from normal male albino rats, of body weight 170-200 g, was fixed by arterial perfusion with buffered tannic acid-glutaraldehyde, and postfixed with osmium tetroxide. Random and isotropic ultrathin sections from 23 different glomeruli from five rats were mounted on slot grids for staining and electron microscopy. Prints of whole glomeruli at a magnification of 3,909 were analyzed by stereological methods. The mean glomerular volume was (8.048 +/- 0.474) X 10(5) mum3 if the glomeruli are treated as spheres. The area of the basement membrane was 0.281 +/- 0.017 mm2 per glomerulus, of which 0.184 +/- 0.011 mm2 represents peripheral basement membrane. The aggregate epithelial slit length per glomerulus was 65.19 +/- 3.84 cm, of which 48.69 +/- 2.87  cm represents epithelial slits abutting on the peripheral basement membrane. Assuming that a slit diaphragm is 390 A wide, and that the pores of the slit diaphragm represent 26% of its area, the mean pore area is 3.96 cm2, of which 2.96 cm2 represents the area of peripheral pores. These findings are discussed in the context of the hydrodynamic theory of glomerular ultrafiltration. We conclude that the porous substructure of the glomerular slit diaphragm is significant in determining the hydraulic conductivity of the glomerulus and hence also solute flux during ultrafiltration.


Intercellular communication in the rat anterior pituitary gland. An in vivo and in vitro study. The concept of "stimulus-secretion coupling" suggested by Douglas and co-workers to explain the events related to monamine discharge by the adrenal medulla (5, 7) may be applied to other endocrine tissues, such as adrenal cortex (36), pancreatic islets (4), and magnocellular hypothalamic neurons (6), which exhibit a similar ion-dependent process of hormone elaboration. In addition, they share another feature, that of joining neighbor cells via membrane junctions (12, 26, and Fletcher, unpublished observation). Given this, and the reports that hormone secretion by the pars distalis also involves a secretagogue-induced decrease in membrane bioelectric potential accompanied by a rise in cellular [Ca++] (27, 34, 41), it was appropriate to test the possibility that cells of the anterior pituitary gland are united by junctions.


Double labeling with [3H]thymidine and [125I]iododeoxyuridine as a method for determining the fate of injected DNA and cells in vivo. Mice were injected intravenously and intraperitoneally with preparations of intestinal nucleoprotein, spleen nuclei, mouse thymus cells, or human kidney T cells whose DNA had been labeled with both [3H]thymidine (TdR) and [125I]-iododeoxyuridine (IUdR). Since free TdR is reutilized more efficiently than free IUdR produced by enzymic hydrolysis of the exogenous DNA, the ratio of [3H]TdR/[125I]IUdR in the DNA fraction of the tissues of the recipient mice provides a measure of the amount of intact exogenous DNA in the tissue. In most instances, the doubly labeled exogenous DNA was almost completely hydrolyzed within 1 day injection, but survival of the DNA from whole cells could be demonstrated in some cases.


Calcium accumulations within the growing tips of pollen tubes. Pollen of L. longiflorum was grown in 45Ca-labeled medium and washed with nonradioactive medium. Whole, labeled pollen was then frozen and autoradiographed at -78 degrees C. The autoradiographs show striking accumulations of 45Ca in the growing tips of the pollen tubes. This result is obtained when the pollen is labeled for times as short as 1 min, or as long as 5 h. In most cases, the tip concentration is about two to four times greater than that in the bulk of the pollen tube, and extends for a length of about 20 mum. In autoradiographs of tubes longer than 1 mm, a small fraction of cells show a distinctly larger 45Ca accumulation, the tip containing more than 100 times that in the rest of the cell. The 1- to 5-h labeling experiments show that calcium is relatively concentrated within the cytoplasm of the growing tip. The 1- to 3-min labeling experiments suggest that calcium may enter the tip faster than it enters other regions. These patterns of calcium accumulation and flux may be related to the localized secretion of vesicles at the grow;ng tip.


Concurrent development of resistance to 6-azauridine and adenosine in a mouse cell line. A variant of the hypoxanthine-guanine phosphoribosyltransferase deficient, and adenine phosphoribosyltransferase deficient mouse resistant to 6-azauridine. These cells are not only resistant to 6-azauridine (5 X 10(-4) M), but also to adenosine (10(-3) M). Resistance persists indefinitely even in the absence of both compounds. The resistant cells are killed by 5-fluorouridine (10(-6) M), indicating that the part of the salvage pathway for pyrimidine ribonucleotide biosynthesis which is relevant to the action of 6-azauridine is intact. The heritable change producing concurrent resistance to 6-azauridine and adenosine probably involves the de novo pyrimidine biosynthetic pathway.


A reduction in energy-dependent amino acid transport by microtubular inhibitors in Ehrlich ascites tumor cells. Vincristine, other periwinkle alkaloids, and colchicine partially inhibit the energy dependent transport of alpha-aminoisobutyric acid in Ehrlich ascites tumor cells. The properties of this phenomenon were characterized in detail for vincristine. Maximum depression of the steady-state intracellular alpha-aminoisobutyric acid level was achieved with a vincristine concentration of less than 0.5 muM. The inhibitory effect of vincristine increases as the extracellular alpha-aminoisobutyric acid concentration is increased reaching a maximum, however, of only approximately to 25% at a level of 5 mM, leaving a large gradient for alpha-aminoisobutyric acid across the cell membrane. Vincristine produced an asymmetrical uptake rate, while increasing the efflux of alpha-aminoisobutyric acid. Inhibition of net alpha-aminoisobutyric acid transport by vincristine was partially reversible (approximately to 40%). Colchicine (50 muM) reduced the steady-state alpha-aminoisobutyric acid level by 30%, an effect that was not reversible. Inhibition by vinleurosine and vinrosidine was comparable to that of vincristine. Addition of glucose to the medium resulted in a small, but significant, decrease in the inhibitory effects of both vincristine and colchicine. The data indicate that these agents inhibit a small component of the uphill transport of alpha-aminoisobutyric acid in Ehrlich ascites tumor cells. The inhibitory effect of vincristine cannot be attributed to an increase in the passive permeability of the cell membrane to this agent. Rather, the data along with other studies from this laboratory suggest that vincristine reduces the energy-dependent transport of alpha-aminoisobutyric acid by either inhibiting cellular energy metabolism or by inhibiting cellular energy metabolism or by inhibiting the coupling of energy-metabolism to the transport of this amino acid and raises the possibility that cellular microtubules play a role in these processes.


Control of normal differentiation of myeloid leukemic cells. VIII. Induction of differentiation to mature granulocytes in mass culture. There are three types of myeloid leukemic cells, IR+D, IR+D- and IR-D-. IR+D+ cells were induced to differentiate to granulocytes in mass culture in liquid medium by conditioned medium (CM) from cultures of lungs from mice injected with endotoxin. About 90% of the leukemic cells were induced to differentiate, 50% to mature granulocytes and 40% to intermediate stages. An efficient induction of granulocyte differentiation was also obtained with CM from primary cultures of rat embryo or human spleen and there was a lower activity with CM from various other sources. IR+D- cells were induced to differentiate to about 20% cells with intermediate stages but not to mature granulocytes; IR-D- cells could not be induced to differentiate to intermediate or mature stages. IR+D+ cells were induced to form intermediate stages of granulocyte differentiation, to phagocytose and to attach to the surface of the Petri dish, three days after incubation with CM. Optimum induction of mature granulocytes required six more days incubation with CM. Mature granulocytes induced from leukemic cells showed cytochemical properties and a morphology in the electron microscope similar to that of normal mature granulocytes. These induced granulocytes did not form leukemiac in animals or colonies in agar. The granulocytes induced from the myeloid leukemic cells, therefore, behaved like normal mature granulocytes.


Cell attachment to collagen: the requirement for energy. It has previously been demonstrated that cell attachment to collagen depends on the presence of a serum-derived cell attachment factor. Two steps in the cell attachment process have been defined. First, the cell attachment factor can bind to collagen in the absence of divalent cations. Secondly, either Ca++ or Mg++ are required for cells to attach to the collagen-cell attachment factor complex. A third requirement for cell attachment to collagen is demonstrated here; namely, cellular metabolic energy. A simple method for the assay of ATP in tissue culture cells is presented.


Isolation and characterization of alkaline phosphatase-constitutive variants from Chinese hamster ovary cells (CHO-k1). Pure clones with high alkaline phosphatase (ALP) activity were isolated from Chinese hamster ovary (CHO-K1) cells which showed no detectable ALP activity. ALP-positive cells appeared at the frequency of 10(-4) in the N-methyl-N'-nitrosoguanidine-treated cell population. With respect for cellular morphology, plating efficiency and several other enzymatic activities, no distinct difference was found between the original CHO-K1 cells and its ALP-positive variants, although some alterations in karyotype were found. Levels of ALP activity in these clones was stably maintained during serial cultivation. Some of the enzymological properties of ALP in the isolated clones were similar to those in bone or kidney.


A comparative study of the effects of lucanthone (miracil D) and actinomycin D on the Chinese hamster cells grown in cultures. Chinese hamster cells of an established clone line grown in monolayers were incubated for up to two hours with either lucanthone (0.3-30 mug/ml) or actinomycin D (0.06-0.10 MUG/ML) AND SUbjected to radioautographic investigations with 3H-uridine during the period of treatment. At concentration of 9 mug/ml lucanthone selectively inhibited the synthesis of nucleolar (ribosomal) RNA while the extranucleolar RNA synthesis proceeded at a high level. Similar results were obtained with 0.08 mug/ml actinomycin D. Protein synthesis and mitotic activity were also affected by lucanthone but the drug did not markedly interfere with DNA synthesis. Lucanthone appeared to be much less effective in cell killing than  actinomycin D and its inhibitory effects on the nucleolar RNA synthesis and other cellular processes proved readily reversible. The results allow to conclude that lucanthone may be useful as a tool for studying RNA synthesis in animal cells.


Growth limitation of 3T3 mouse fibroblasts by available growth surface area and medium components. Studies with untransformed fibroblasts demonstrate that growth of these cells in culture can be limited by the availability of both growth surface and medium components. Experiments using cells grown on coverslips, in which the only variable was available growth surface, indicate that when the medium to cell ratio is high, surface area is the principal factor limiting growth. At low medium to cell ratios, however, growth of cells is predominantly limited by medium components. The final number of cells per culture is almost directly proportional to available surface area when the culture medium is changed daily.


N6-(delta2-isopentenyl)adenosine, an inhibitor of cellular transport of uridine and cytidine. N6-(delta2-Isopentenyl)adenosine (IPAR) inhibited severely the incorporation of uridine and cytidine into S-180 cells in culture. When IPAR and the nucleosides were simultaneously present in the medium the inhibition was competitive (Ki 3.4 muM) and indicated inhibition of transport. However, the inhibition occurred even in the absence of extracellular IPAR if the cells had been preincubated with IPAR. Since 5'-IPAMP was the product which accumulated in large quantities in S-180 cells when incubated with IPAR, the effects of this AMP analog of the intracellular metabolism of uridine had to be considered. No direct correlation between the amount of intracellular IPAMP and the degree of inhibition of uridine utilization was observed and the relative distribution of uridine nucleotides in the acid soluble pool of the cells was unaltered in cells treated with IPAR. Also, IPAMP was not an inhibitor of uridine kinase in a cell free system nor was the activity of this enzyme affected by treatment of cells with IPAR. In addition, a profound inhibition of uridine utilization was also observed in a resistant subline of S-180 cells, which is unable to form IPAMP. These data suggest that IPAMP was not the inhibitory agent. Furthermore, the observation that the inhibition in both sensitive and resistant cells was caused even by a 15-second exposure to 100 muM IPAR, followed by rinsing, suggests that IPAR itself is the effective agent. It is concluded that IPAR exerts its inhibitory effect on uridine and cytidine utilization by becoming lodged in the cell membrane and thereby preventing the passage of these nucleosides into the cells. It is also shown that the inhibition of uridine and cytidine utilization by IPAR and by other potent nucleoside uptake inhibitors is unrelated to inhibition of growth or RNA-synthesis when the cells do not depend on an extracellular source of a nucleoside for growth.


Effects of withdrawal of a serum stimulus on the protein-synthesizing machinery of cultured fibroblasts. 3T6 cells resting in medium containing 0.5% serum were stimulated to prepare for multiplication by the addition of medium containing 10% serum. After a number of hours, when the rate of preribosomal RNA synthesis, total RNA content (mainly ribosomal), and the cytoplasmic content of poly A (a measure of poly A(+) mRNA) were considerably elevated, the serum-rich medium was withdrawn, and the original medium replaced. The rate of preribosomal RNA synthesis began to drop within 30 minutes, but required a much longer time to fall to a new resting level. When the serum-rich medium was withdrawn after 12 hours of stimulation, the total RNA content required 12-18 hours to fall to the resting level, whereas cytoplasmic poly A content and the rate of protein synthesis declined more rapidly, reaching a new resting level within eight hours. During the 12 hours following withdrawal of the serum-rich medium an appreciable fraction of the cells initiated DNA synthesis. Presumably, the cellular preparations for DNA synthesis cannot be immediately reversed because of the inertial factors related to the protein-synthesizing machinery.


Growth enhancement of myogenic tumor cells by conditioned medium from embryo fibroblasts. Growth medium was conditioned by incubation on mouse embryo cells in vitro. Supplementation of agar suspension cultures with conditioned medium from primary cells, but not from established lines, readily enhanced colony development by mouse tumor cells. Only cells with the properties of myoblasts responded to conditioned medium. Other fibroblastoid cells and virus-transformed cell lines were not affected. Myogenic cells in agar cultures grew in the presence of conditioned medium but did not differentiate. Soluble collagen at 400 mug/ml possessed little colony-stimulating activity by comparison with fresh conditioned medium.


Myosin in developing normal and dystrophic chicken pectoralis. I. Synthesis and degradation. The synthesis and degradation of pectoralis myosin have been investigated in chickens 15-23 days after hatching. An essentially nonreutilizable tracer amino acid was used to demonstrate differences in the rates of synthesis and degradation of myosin isolated from normal and hypertrophied, cystrophic breast tissue. The analyses have shown that the dystrophic system  synthesizes myosin faster than the normal system and that only myosin in dystrophic pectoralis is degraded during the experimental period. Double label experiments have indicated that the heavy chains of dystrophic myosin are destroyed at a rate greater than that characteristic of the light chains.


Myosin in developing normal and dystrophic chicken pectoralis. II. The relationship between intracellular and extracellular aspartate pools and myosin synthesis. The present studies establish the cellular pool of aspartate as the major source of this amino acid used during the biosynthesis of skeletal muscle myosin. The precursor=product relationship has been established in growing, normal pectoralis and has been suggested in hypertrophied, dystrophic pectoralis. Specific activities of aspartic acid in plasma and cellular pools corrected for extracellular space contributions have been correlated with aspartate incorporation onto myosin. These data have been coupled with quantitative data on myosin accumulation and have established the cellular pool as the major precursor pool. The data also give further insight into some of the factors responsible for the observation that in vivo dystrophic tissue gives higher levels of aspartate incorporation than normal tissue.


Decreased 3H-uridine incorporation and increased 3H-adenosine incorporation by HeLa cells exposed to autologous culture fluid. Actively growing HeLa monolayer cultures briefly exposed to the culture fluids (CF) from confluent HeLa cultures and labeled simultaneously or subsequently, incorporated less 3H-uridine (3H-UR) but more 3H-adenosine (3H-AR) than control cultures similarly exposed to fresh medium and labeled. Exposure to CF inhibited the uptake as well as the incorporation of 3H-UR by cultures. The inhibition of 3H-UR incorporation by CF-exposed cultures could be reduced by increasing the concentration of 3H-UR in the labeling medium. Both the inhibition of 3H-UR incorporation and the stimulation of 3H-AR incorporation were prevented by washing the CF-treated cultures with phosphate buffered saline before labeling. Similarly, both effects could be producted in HeLa cultures exposed to fresh medium containing 1 X 10(-5) M uridine instead of to CF. Therefore, the observed effects of CF on label incorporation were probably due to the presence of uridine or a related compound, and the inhibition of 3H-UR incorporation resulted from reduced uptake of 3H-UR rather than from reduced RNA synthesis by exposed cells. The active agent in the CF, formed only when cultures were incubated at physiological temperatures, was not a product of medium decay. It was a cellular product formed equally well by cultures incubated in medium containing dialysed or whole serum.


The kinetics of serum-induced initiation of DNA synthesis in BHK 21/C13 cells, and the influence of exogenous adenosine. After the re-addition of serum in the presence of adenosine (25 muM), the entry of quiescent, serum starved BHK 21 cells into DNA synthesis follows first order kinetics after a well defined lag period of eight hours, and with a rate constant dependent on serum concentration. Initiation of DNA synthesis under these conditions can therefore be considered to be a random event occurring with a "Transition Probability" determined by the serum concentration. In the presence of adenosine, the change of Transition Probability following the addition of serum occurs abruptly. In the absence of exogenous adenosine, however, the change of Transition Probability after serum addition appears to be both gradual and bi-phasic. The initial changes in the absence of adenosine, though smaller in magnitude, display a similar dependence on serum concentration to the changes occurring in the presence of the nucleoside. In contrast, the secondary gradual increase of Transition Probability in the absence of added purines exhibits a higher serum requirement. It is suggested that the regulation of Transition Probability by serum involves some purine-dependent process, and that in the absence of an exogenous supply this becomes limited by endogenous synthesis which in turn may be dependent on serum concentration.


Anchorage dependent changes in transport of glucose, adenosine, uridine and leucine in 3T3 cells. There is a low uptake of leucine in suspension cultures of 3T3 cells relative to the uptake in sparse monolayer cultures. The pattern of uptake of deoxyglucose is similar in suspension and monolayer cultures and changing the medium or adding serum stimulates uptake under both culture conditions. The uptake of uridine and adenosine is greater in suspension culture than in monolayer culture. Cells do not multiply in suspension culture but do multiply in monolayer culture and thus there is a correlation between uptake of leucine and conditions which stimulate cell multiplication, but not correlation of the uptake of deoxyglucose, uridine and adenosine with these conditions.


Non-mendelian segregation in hybrids between chinese hamster cells. Mechanisms of segregation have been examined in hybrids between Chinese hamster cells, where chromosome loss in comparison to other systems is minimal. Hybrid cells were grown in HAT medium and subjected to back selection with bromodeoxyuridine (BUDR) or azaguanine (AZG). In AZG or BUDR at 30 mug/ml, segregation began with a random high frequency event  that gave rise to cells capable of growth in both HAT and back selection medium, unlike the precursor hybrid or original parental cell types. BUDR-resistant segregants were propagated serially in the presence of BUDR, and were examined by clonal analysis for changes in plating properties during long term culture. Over a period of 300 days the HAT/BUDR plating ratio for sergregant cells declined continuously. A parallel decrease was observed in the rate of H3-thymidine incorporation, along with a drop in thymidine kinase activity. These shifts took place only in the presence of BUDR, and could be reversed by altered selection in HAT medium. Clonal studies showed that the evolution of segregant properties occurred in most if not all cells of the population, and did not arise from variation and selection of minority cell types. These properties of the segregating system are not consistent with models based on gene mutation, chromosome  rearrangements, or chromosome loss. The evolution of segregants resembles more closely a sorting-out progress, taking place by intracellular selection over many generations. The segregating units may conceivably be cytoplasmic determinants linked functionally to nuclear genes, and which serve to modulate the events of phenotypic expression. Several lines of evidence which bear on this concept are discussed.


Chromosomal proteins of rat brain: increased synthesis and affinity for DNA following a pulse of the carcinogen ethyinitrosourea in vivo. A single pulse of ethlnitrosourea (EtNU), administered to 10-day-old BD IX-rats, specifically results in a high incidence of neuroectodermal tumors in the central and peripheral nervous system. At five days after an EtNU-pulse, analyses of protein-DNA interactions were performed using chromatin dissociation and re-association experiments, following incorporation of radioactive leucine into brain chromosomal proteins (CP) during short-term suspension culture. In comparison with 15-day-old control animals, the brain cells of EtNU-treated rats exhibited (i) an increased rate of CP synthesis, and (ii) an increased affinity of the newly-synthesized CP for brain DNA of both control and EtNU-treated animals.


A method of structure-activity correlation using Wiswesser Line Notation. Fragment sets generated manually from Wiswesser Line Notation have been used to correlate the chemical structures of a group of 79 penicillins with their serum binding activity, using multiple regression analysis. Statistically significant correlations were found, with results in accordance with the generally accepted nature of the binding. Algorithmic methods for the generation of such fragment sets are proposed and the use of various structural representations for structure-property correlation within chemical information systems is discussed.


Developing drug literatures. 1. Bibliometrics of baclofen and dantrolene sodium. The literatures of two antispastic drugs, baclofen and dantrolene sodium, were studied bibliometrically for their first decade and were found to be generally similar. Baclofen had 93 papers and dantrolene sodium had 70. About a quarter of the baclofen papers were in a foreign language, whereas almost all dantrolene sodium papers were in English. Baclofen literature had a lower nonscholarly content, but the scholarly increase, 5%, was the same for both. Both drugs had an average of 12 references per paper. The literatures of both drugs had a similar degree of internal cohesiveness; almost half of their papers referred to other papers in the same drug collection. The first human paper for baclofen was the second published; for dantrolene sodium, it was the eleventh. The distribution of journals carrying papers on each of these drugs followed Bradford's law. Two authors per paper was the average for each drug. The productivity of authors approximated Lotka's law for both drugs. About two-thirds of the papers of both drugs had a drug-word in their titles. The literature of both drugs contained about 15% legendary papers, typical of clinical pharmacology. The most intense papers, 15 for baclofen and 11 for dantrolene sodium, were identified, using citation, bibliographic coupling, and co-citation frequencies. A generalization predicts what might be expected from the literature of future antispastic drugs.


Specialized reference works and their users. A preliminary study. An examination of the use of three specialized reference works was made to identify users of the reference tools and the benefits from their use. The feasibility of locating and interviewing users was tested, and an attempt was made to determine the situations in which the works are used. Methods of locating users are described. Users were interviewed to determine the value of each work and alternative sources of information. The works were compared with alternative sources to test their comprehensiveness. Conclusions are: (1) identification of users and their needs is complex, and requires the combination of a number of research methods; (2) the availability and accessibility of alternate information sources varies with both the reference work and the type of user.


A survey of the use of on-line computer-based scientific search services by academic libraries. To explore their use of on-line computer-based bibliographic search services, a one-page questionnaire was sent to 100 academic libraries in the United States having separate departmental chemistry or science libraries. An attempt was made to determine the background training of the persons performing the searches, who the end users were, the growth trend, the funding, the data bases used, and the value to the users. Of the 73 replies, 49.3% indicated use of such services, while 24.6% were planning to use them primarily by faculty and graduate students. Of those reporting, 83.3% used searchers with a background in library or information science; 47.2% had a background in scientific discipline. Two or more on-line services were used by 72.2%. A majority of respondents stated that the user paid all expenses or a portion of the expenses. The searches met the needs of the user most of the time in 83.3% of the cases, and all of the time in 5.4%.


[Indications for re-operation for infectious complication of abdominal surgery]. The onset of infective phenomena following abdominal surgical operations requires a full assessment based on the danger of septic intraperitoneal focus, which can only be cured by surgery. The indications for re-operation depend on the presence, or absence, of two types of symptom: Firstly, the existence of local signs, such as peritonitis, intestinal obstruction, whether clinically or on XRay. Furthermore, the onset of general signs, such as septicemia, septic shock, acute organic renal failure, or interstitial pneumonia, may lead to re-operation, even in the absence of local abdominal signs, provided one has eliminated any obvious extra-peritoneal septic focus.


[Embolism in healthy arteries].

[Embolism in healthy arteries]. The authors present a series of 64 patients with arterial embolism in healthy arteries. 53 of these patients underwent one or several embolectomies, using a Fogarty catheter. Although arterial embolism has a poor prognosis owing to the constitutional background (15 p. 100 mortality and 12 p. 100 amputations), this is mainly due to the age of the patient. The severity of the initial attack and the delay between embolism and embolectomy seem to be the main factors in prognosis. Massive ischemia causes severe symptoms in these fragile patients and an early cure is necessary to compensate this disturbance. Heparin perfusion, whilst awaiting surgical treatment, is essential. Embolectomy by Fogarty's catheter may be carried out under local anesthesia; this remains the essential measure and has greatly improved the prognosis of this disease which used to be fatal in almost 60 p. 100 of cases, even in healthy arteries.


[Experimental portal hypertension in the dog]. Portal hypertension may be obtained in the dog at three levels: by blocking the venous circulation either above, within or below the liver. Portal hypertension by venous obstruction below the liver is obtained by partial ligature of the portal vein using a ring covered with talcum powder. The fibrous reaction thus created causes occlusion of the portal vein in 50 p. 100 of cases. This portal hypertension is transient and is not accompanied by oesophageal varices. The special anatomical lay-out of the portal system in the dog, explains these findings.


Rapid and selective determination of free barbiturates by gas chromatography using the electrolytic conductivity detector. A rapid and specific gas chromatographic method for the analysis of free barbiturates in serum and urine at all levels of clinical interest is described. The method employs an improved electrolytic conductivity detector, and can be used for the determination of barbiturate levels of 0.01 mg/dl without sample clean-up. The preparation of columns for the analysis of nanogram quantities of free barbiturates is also discussed.


[Surgical treatment of autonomous chronic pancreatitis (apropos of 147 cases)]. Surgery in chronic pancreatitis gives only poor results: 40 p. 100 of cures or long-term improvements. Apart from certain mistaken indications, three factors are associated with a poor prognosis: calcification, late operation, persistence of alcoholism. However, mechanical complications, such as jaundice, pyloric stenosis, digestive hemorrhage and pseudo-cyst, are the main undoubted indications for operation. The choice of operation depends mainly on the state of Wirsung's duct. Usually, either part of the gland is removed or an internal digestive fistula is performed. Removal of the left lobe of the pancreas gives good results. Cephalic duodeno-pancreatectomy which is satisfactory in the short term, does not give, in the long run, better results than those of drainage operations. Pseudo-cysts are treated by removal when situated in the body or the tail and a drainage operation in the cephalic form. Simple operations should be preferred.


Chromatographic analysis of hydrocarbons in marine sediments and seawater. The low concentration of hydrocarbons anticipated in pollution baseline studies necessitates the development of analytical techniques sensitive at the sub-microgram per kilogram concentration level. The method of analysis developed in this laboratory involves dynamic headspace sampling for volatile hydrocarbon components of the sample, followed by coupled-column liquid chromatography for the non-volatile components. These techniques require minimal sample handling, reducing the risk of sample component loss and/or sample contamination. Volatile sample components are separated from the matrix in a closed system and concentrated on a TENAX-GC packed pre-column, free from large amounts of solvent and ready for GC/GC-MS analysis. Non-volatile compounds, such as the benzpyrenes, may be extracted from large volumes of water and concentrated on a Bondapak C18 packed pre-column for coupled-column liquid chromatographic separation and analysis. Results of the application of these techniques to the analysis of samples from sites of known low level hydrocarbon contamination are presented and discussed.


Reverse phase thin-layer chromatographic separation of bromodiphenhydramine and diphenhydramine. A thin-layer chromatographic system is described for the separation of diphenhydramine and bromodiphenhydramine which is based on a reverse phase paper chromatographic system developed by J. Vecerková (2,3) in 1962. Chromatographic systems for this separation described in the literature and several systems attempted in this laboratory using normal chromatography have not proven successful. The method using reverse phase thin-layer chromatography involves a stationary phase of mineral oil on silica gel G and a mobile phase of ethanol:water:ammonium hydroxide, 28% NH3, (55:43:2). A 10-cm length of run requires about three hours and provides an excellent separation of the two compounds for identification-differentiation purposes.


TLC screening techniques for the qualitative determination of natural and synthetic capsaicinoids. Three TLC methods of qualitative screening of capsicum products are described. A procedure using a two-dimensional reversed phase system and bromination of the sample gave the greatest flexibility but required the longest time. A one-dimensional reversed phase system using silver ion to complex with the unsaturated components is also described. Polyamide plates using silver ion in the developing solvent gave the greatest speed of the three methods compared.


Preparation of superior thin-layer chromatograms for quantitative in situ densitometry by nonequilibrated development. I. Trisulfapyrimidines: sulfadiazine, sulfamerazine, and sulfamethazine. A method of obtaining controlled nonequilibrated horizontal plate development by employing a developing system open to room atmosphere is described and compared with other plate-developing techniques. Nonequilibrated plate development produced spot configurations with the precise physical outline and densitometric distribution needed for optical scanning.


Luminescence analysis of anti-inflammatory agents in blood or plasma following thin-layer chromatographic separation. The luminescence properties of some anti-inflammatory agents and of 6-chloro-1,2,3,4-tetrahydrocarbazole-2-carbox-ylic acid, 6-chloro-9-[2-(2-methyl-5-pyridyl)ethyl]-1,2,3,4-tet-rahydrocarbazole-2-methanol.HCl, and (d,l)-6-chloro-alpha-methyl-carbazole-2-acetic acid have been examined at both ambient and cryogenic temperatures. Assays were developed for indomethacin, the tetrahydrocarbazoles, and the carbazole based upon solvent extraction from blood or plasma, thin-layer chromatographic separation of the drugs from interfering materials and phosphorimetry of the eluted materials. The solvent elution may be accomplished either by manual scraping and elution or by the use of a semiautomated elution apparatus. These two techniques were compared with respect to overall recovery and precision using both fluorometry and phosphorimetry.


Thin-layer and gas chromatographic screening programs for new drug substances. Procedures are described for evaluation of the thin-layer and gas chromatographic properties of new raw material drug substances over a broad spectrum of conditions. Chromatographic profiles are established for each drug, and these are used as starting points for analytical procedures for the drug, its synthetic precursors, and its degradation products. Thin-layer chromatography is performed initially by use of three absorbents and a series of six solvents. Gas chromatography is performed initially by temperature programming on a series of six different columns, followed by isothermal experiments. Evaluation of resulting chromatograms from both techniques determines whether more experiments are necessary or whether an analytical method can be developed based directly on one of the results of the initial screening.


[Direct approach for bypass of the distal deep femoral artery. The supra mediocrural route]. The medio-femoral route permits a simple, direct approach to the distal part of the profunda femoris artery outside Scarpas triangle, i.e. below the first perforating artery. The quadriceps femoris and sartorius muscles are separated. This segment may reach the lower part of the popliteal artery through various collaterals supplying the muscle and nerve arteries and, hence, the leg. In selected cases with severe ischemia and absent femoral pulse, a by-pass operation carried out at this level permitted one to obtain a satisfactory arterial blood supply to the leg and foot. Used in only three patients, with a follow-up of several months, we cannot yet draw final conclusions, but the quality of the early results is evidence for the interest of the method.


[Results of primary reconstructive arthrodesis of the calcaneus (study of a homogenous series of 55 fractures of the medical surface of the calceneus)]. 55 cases of calcanear fractures (thalamus) have been treated with the Stulz procedure (early subtalar arthrodesis and calcanear reconstruction), associated with a repair of the great apophyse. All the cases are comparable and the results are considered as good or excellent in 93,8 p. 100 of them. The results are interpreted accordingly to subjective and objective tests. The result is depending of the type of the lesion, the patient's profession and the nature of the trauma. The result obtained at the time of fracture uniting will stay stable, will never deteriorate and sometimes improve. The early arthrodesis and reconstruction remains a good procedure for the comminutive fractures of the thalamus, which are considered as the most difficult to correct perfectly. For this reason, the simple reconstruction is often responsible of secondary subtalar arthrosis.


Comparison of the counter-immunoelectrophoresis technique with the Reiter protein and three other serological tests as a first line test for syphilis. The counter-immunoelectrophoresis technique with the Reiter protein (RP-CIE) was compared with two complement fixation tests (Kolmer and RPCF) and a flocculation test (VDRL) in sensitivity and specificity. Of the 1,927 consecutive attendants of a venereal disease clinic whose serum samples were used, 250 were considered to be syphilitic. The number of true-positive and false-positive reactions were: 121 and 4 (VDRL), 124 and 2 (Kolmer), 179 and 41 (RPCF), 166 and 16 (RP-CIE). The VDRL and the RPCF combined were more sensitive and less specific than the VDRL combined with the Kolmer. If the RPCF was replaced by the RP-CIE the sensitivity remained the same but the specificity was higher. The RP-CIE scored more positives than the RPCF in untreated cases of primary syphilis. The results argue for substitution of the RP-CIE for the Kolmer in the combination with the VDRL in the serodiagnosis for syphilis. Moreover, the RP-CIE presents the technical advantages of simplicity, speed of performance, and of not being hampered by the anticomplementary nature of the serum sample.


Rapid separation of immunoglobulin M from immunoglobulin G antibodies for reliable diagnosis of recent rubella infections. Chromatography on controlled pore glass was adapted for the separation of immunoglobulin M (IgM) and immunoglobulin G (IgG) rubella antibodies from 0.3-ml samples of human serum. An extremely sharp separation of IgM from IgG antibodies could be obtained within 40 min. Nonspecific inhibitors were removed before chromatography by precipitation with high-molecular-weight dextran sulfate, and the titer of rubella antibodies in the different classes of immunoglobulins were assayed with a modified hemagglutination inhibition technique. The combination of these methods is recommended for routine tests. It permits an accurate diagnosis of recent rubella infection within a few hours.


Seroepidemiological investigation of Brucella canis antibodies in different human population groups. A study was conducted to determine the prevalence of Brucella canis antibodies in specified groups based on their exposure to dogs. The method used was a microtiter technique, and the presence of antibodies at a 1:12 or greater dilution of serum was considered a positive test. Eleven (5.7%) of the newborn infants had evidence of maternal antibodies, and 67.8% of the persons with an average exposure to dogs had B. canis antibodies, with a 62.1% prevalence in males and a 72.4% prevalence in females (P less than or equal to 0.001). Veterinarians had a much higher rate of infection (72.6%) than male blood donors (56.9%) (P less than or equal to 0.01). Patients with fevers of undetermined origin had significantly higher antibody titers to B. canis than all other patients (P less than or equal to 0.001). This study presents evidence that the prevalence of B. canis antibodies in humans is high, and that the incidence of brucellosis may increase when physicians consider B. canis as a possible etiological agent in febrile illnesses.


Discriminant analysis of antibiotic susceptibility as a means of bacterial identification. This study shows that antibiotic susceptibility data can be used effectively in the presumptive identification of bacteria. Using 12 antibiotics and determining the zone sizes for each, 82% of the isolates considered were correctly identified without any other information. If the inability to distinguish between Escherichia coli and Shigella is disregarded, the percentage of correct identification is 92%. The method involves determining a set of discriminant functions and defining each taxon by a unique function. An unknown isolate is identified by evaluating each discriminant function and assigning the isolate to the taxon whose discriminant function has the largest value. A total of 468 isolates were examined. After eliminating the multiply resistant isolates, the remaining 369 isolates were used to determine the discriminant functions for the eight taxa considered.


Relationship of indole production and antibiotic susceptibility in the Klebsiella bacillus. Of 2,442 Klebsiella strains isolated from clinical specimens at the University of Minnesota hospitals, 18.3% were found to be indole positive. A randomly selected equal number of indole-positive and indole-negative control isolates, characterized by 27 biochemical tests and by serotyping against 72 antisera, were tested against 14 antibiotics. The results indicated a greater incidence of multiple drug resistance among the indole-negative strains among those that produced indole. The organisms in the former group, in comparison to their indole-positive counterparts, were significantly more resistant to nitrofurantoin, tetracycline, chloramphenicol, neomycin, streptomycin, nalidixic acid, and kanamycin. Both groups of organisms were similar in their degree of resistance to ampicillin, carbenicillin, cephalothin, sulfisoxazole, colistimethate, polymyxin B, and gentamicin. The biochemical properties of the two indole groups were essentially identical. Correlation between serotype and multidrug resistance was inapparent.


Unusual colonies of Ureaplasma urealyticum (T mycoplasmas) in primary agar cultures of certain urine specimens. The existence of unusual colonies of Ureaplasma urealyticum (T mycoplasmas) in primary agar cultures of certain urine specimens is reported and their morphology is illustrated.


Color reaction streak test for catalase-positive microorganisms. A stable purple color results when a reagent solution is applied to a smear of catalase-positive organisms streaked on a glass slide.


Effects of phenothiazines and social skills training in a withdrawn schizophrenic. The effects of phenothiazines and social skills training on a severely withdrawn schizophrenic were examined. Regulation of phenothiazine levels had the result that the patient became more receptive to behavioral interventions. Specific application of a token economy program effected a marked improvement in the assertive training was employed to improve the patient's repertoire of ininterpersonal responses. Prior to actual hospital discharge the patient was faded back into his natural environment during a job retraining phase at Goodwill Industries. Specific data on the patient's improved social functioning are presented in a multiple baseline analysis. Follow-up data document the patient's successful adaptation of his natural enviroment.


The effect of severity, consistency, and typicalness information on clinicians' causal attributions. Sixty-four clinicians responded to a brief description of a depressed student by indicating the extent to which each of four possible causes contributed to the depression. The descriptions were varied systematically so that the student's depression was described as either mild or severe, typical or atypical, and as having occurred before or as never having occurred before. Results indicated greater attribution of the depression to internal causal factors when the individual's depression was described as atypical and greater attribution to stable causal factors when the depression was described as having occurred previously. When the depression was described as severe, the clinicians tended to attribute it to external unstable causes. It was suggested that clinicians may differ from other populations in some of the causal attributions that they make for psychological difficulties.


Paranoid integration and thought disorder. This study investigated the relationship between thought disorder as measured by Bannister's Grid Test and paranoid integration. Twenty-six hospitalized schizophrenics were subdivided into High Integration and Low integration groups on the basis of scores on Foulds' System-Sign Inventory. No significant differences were found between these groups on the thought-disorder measure. When the sample was subdivided into paranoid and nonparanoid groups on the basis of clinical diagnosis, paranoids were found to be significantly less thought disordered than nonparanoids.


A factorial approach to neuropsychological assessment. A factorial structure approach is examined in the assessment of test behaviors related to brain damage. A factor analysis performed on data from an extensive battery of psychological tests administered to 292 adult brain-damaged patients yielded five clearly defined factors. Multiple regression analysis was employed to predict performance on a second set of criterion tests that used, first, actual test scores, and second, factor scores. Formulae are presented to predict factor scores from the few best contributing variables. These estimated factor scores applied on independent patients indicate predictability through the use of raw test scores as good as that derived through the use of estimated factor scores. Results reveal an economical and comprehensive method to assess brain functioning through the use of factor categories rather than isolated test scores.


Personality, body image, and eating pattern changes in overweight persons after weight loss. A group of overweight persons who achieved a substantial weight loss over a 6-month period were compared with a group of overweight persons who showed an unsubstantial weight change and a normal weight control group. All groups were evaluated before the weight program began and at a 6-month follow-up. The substantial weight loss group showed a number of significant changes after weight reduction. Body image rating shifted in the direction of evaluating onself as less heavy, eating was rated as less good and more heavy, and a greater feeling of self-control was indicated. Over time, the change group learned to limit the number of emotional and environmental cues that were discriminative stimuli for eating behavior. There also were more frequent reports of eating in response to cues of hunger after substantial weight loss.


The dependence of psychiatric diagnosis on psychological assessment. This study examined the dependence of 500 inpatient psychiatric diagnoses on the results of standard psychological assessment procedures. A 43% agreement was found between psychological test findings and psychiatric discharge diagnoses. While this level of agreeement is greater than chance, it suggests that psychological test results are not of value in more than one-half of the cases investigated. Specific diagnostic discrepancies between clinical and psychological diagnostic results were noted, and several explanations for these differences were offered.


Depression and senility.

Depression and senility. The literature suggests a closer relationship between reversible depression and irreversible senile organicity than generally has been accepted. Accordingly, Zung's Self-Rating Depression Scale was given to 120 men and women, aged 60 or older, of whom 30 of each sex were classified as nonsenile and 30 of each sex were classified as moderately senile by the personnel of senior citizen centers, convalescent hospitals, and nursing homes. The results indicated that moderately senile Ss scored significantly higher on the SRDS than did the nonsenile Ss, that sex differences were not significant, that there were no significant differences between institutionalized and noninstutionalized Ss of either sex in either the nonsenile or the moderately senile groups, and that further refinement of the SRDS and a longitudinal study of personality, experience, and depression would be extremely useful.


A study in childhood egocentricity.

A study in childhood egocentricity. The SFSC was administered to 79 children and 63 adolescents who were considered to be functioning at a normal level. The ratio of self-focus to environment focus scores was related significantly to age, which confirms a hypothesis based upon Piaget's theory of mental development. Deviations from a relatively equal S/E ratio have been associated previously with psychopathology in adults. This was not found in the present study. It was suggested that an even S/E ratio may be inappropriate for children and youth, whose response styles are still in a state of development.


Increase of personality response consistency by prior response. Twenty-nine Ss were given the MMPI weekly for 4 weeks. Experiments 1, 2 and 3 reported data that support each of the following specific predictions: (1) scale reliabilities over trials 3-4 will be greater than over trials 1-2; and (3) correlation of standard score patterns over trials 3-4 will be greater than similar correlations over trials 1-2. These results, as well as other literature, support the general hypothesis that personality response consistency is increased by prior response. A negatively accelerated decreasing curve of novelty was proposed to account for this general hypothesis. In addition, split-half reliability and test-retest reliability showed moderate intercorrelation. Moderate support also was derived for Windle's generalization that maladjustment scores decrease on retest.


Validation of a brain-damage vs. schizophrenia MMPI key. Since neuropsychologic tests largely have failed to separate schizophrenic from brain-damaged patients, an MMPI key was constructed to make such a separation. When cross-validated, this key provided a 76% correct separation and achi2 significant beyond the .001 level for 50 schizophrenic and 50 brain-damaged profiles. Neuropsychologic tests should be used to separate normals from brain-damaged patients and the MMPI to separate schizophrenic from brain-damaged patients.


Introversion-extraversion and topological representations of self and others. This study indicates that the vertical ordering of self-social others is a potent measure of individual differences in social orientations. Since the extravert is the more sociable, excitement-seeking, carefree individual, while the introvert is more retiring, aloof and introspective, it would be worthwhile in future research to determine whether the dominance, vs. submissive or the high vs. low status dimension is the essential correlate of these spatial differences.


EPI and IPAT anxiety scale performance in young women as related to breast feeding during infancy. Performance of female first-year psychology students on the EPI and IPAT anxiety scale were examined in relation to length of breast-feeding during infancy. Ss who never were breast fed scored significantly higher on the IPAT scale and the EPI neuroticism dimension than breast-fed Ss irrespective of duration. The results were discussed in the light of future investigations of mother-child relationships.


Prisoner intellectual and personality correlates of offense severity and recidivism probability. Multiple regression analyses were performed relating the severity of offense and probability of recidivism of 295 prison inmates to their performance on the MMPI and AGCT. The MMPI was found to be correlated with recidivism probability and the recidivism-related component of the Offense Severity variable, while AGCT IQ was found to be correlated with recidivism probability and the non-recidivism-related component of the Offense Severity variable. The implications for future research were discussed regarding this selective sensitivity of the MMPI to recidivism-related variables.


Relationship between the Mini-Mult and the MMPI with medical patients. The correspondence between the MMPI and a shortened version of the MMPI, the Mini-Mult, was examined with 50 male and 50 female medical outpatients. The results indicated that the individual scales of the Mini-Mult produced results highly similar to those produced by the standard MMPI scales. As a screening device in a medical setting, the Mini-Mult appears to be a farily accurate substitute for the MMPI.


Relationship between the Death Anxiety Scale and repression. Fifty female undergraduate Ss participated in a study designed to test the hypothesis that the DAS is a valid measure of repression. The results supported the hypothesis and provided an indication that the repression of feelings is the major aspect that accounts for the relationship between the DAS and repression as measured by Gleser and Ihilevich's Defense Mechanism Inventory.


The sensation seeker and anxiety reactivity: relationships between the sensation-seeking scales and the activity preference questionnaire. The five scales of the Zuckerman Sensation-Seeking Scale and Lykken's Activity Preference Questionnaire were administered to 83 male and female college students to test the hypothesis that there is an inverse relationship between sensation seeking and anxiety reactivity. The data were intercorrelated separately for males and females. Results were, in general, consistent with expectations: individuals high in optimal level of stimulation are low in anxiety reactivity in situations that involve physical danger and in situations related to social fears or embarrassment. This relationship was found for most of the dimensions of sensation seeking for males, but was confined to specific subscales for females.


Relationships among locus of control, self-concept, and anxiety. Sixty male alcoholic inpatients were administered the Locus of Control scale (IE), the Activity Preference Questionnaire (APQ), the Manifest Anxiety scale (MAS), and the Tennessee Self Concept scale (TSCS). Ss defined as having an external locus of control appeared significantly more anxious than internal Ss on the MAS; however, no differences were found between groups on the APQ or TSCS scales. The self-report (MAS) and nonobtrusive (APQ) measures of anxiety were not correlated significantly. A differential pattern of correlations was found between the MAS and APQ and TCSC subscale scores. The obtained correlations support the contention that the MAS may measure more accurately a dimension of neuroticism and/or negative self-concept rather than anxiety. Further research appears necessary to investigate the internal consistency and dimensionality of the MAS.


The MMPI in the differential diagnosis of organicity vs. schizophrenia: empirical findings and a somewhat different perspective. Watson's MMPI Scale for differentiating organicity from schizophrenia was found to correlate significantly with the MMPI Schizophrenia Scale and was found not to differentiate significantly better than the Schizophrenia Scale for the two patient groups under consideration. Watson's general approach of using personality measures for such differential diagnosis was lauded, although doubt that his scale is superior to other methods of diagnosing schizophrenia was expressed.


Intellectual functioning and psychopathology: a canonical analysis of WAIS and MMPI relationships. This study investigated the relationships between WAIS and MMPI scales in high pathology-average intelligence outpatients referred for psychological evaluation. No significant relationships were obtained. Apparently, no lawful relationships exist between WAIS and MMPI scales, and further investigation seems unwarranted.


Wechsler memory scale indices of brain pathology. The present study reported on preliminary findings of two experimental forms of Wechsler Memory Scale subtest analyses that show promise as screening procedures for neurologically impaired Ss. Both methods discriminated the organic Ss for controls with 70% accuracy. Both methods compare "hold" subtest scores to "don't hold" subtest scores by the use of a regression equation, a procedure that attempts to compensate for the lack of standard scores. The reasons why the regression equation that uses digits score as a hold criterion is regarded as potentially the more useful were discussed.


Correlates of initial client disturbance: expectations for therapy, dropout, resistance, and demographic description. Relationships were investigated between initial levels of client disturbance in each of 10 problem areas and client drop-out, lateness to, and absence from therapy, expectations with regard to therapy, and various demographic variables. Data were obtained from 154 outpatients at a university psychological clinic. Correlations computed between initial disturbance scores and all other variables revealed many significant relationships. Most noteworthy were the significant relationships betwee initial disturbance levels in the areas of future goals and self-satisfaction and expectations in a psychodynamically oriented therapist. Results were discussed in terms of client's awareness of the need to deal with the psychological factors that underlie their problems and their desire for the therapist to focus on such material.


Self-esteem, locus of control, and approval motivation in married couples. The authors feel that the two observations in this study of greatest importance to researchers and practitioners alike are the sex difference observed in homogeneity of self-perception and the significant correlation between SEI and MCSD. In the case of the former observation further research will be necessary to clarify this difference, but a good starting point would be to establish whether it can be replicated on college students. There may be a generational shift in this phenomenon, and it will be necessary to establish this observation before further analysis is possible. The implications of the second observation have been discussed. Of course, independent confirmation is necessary in order to substantiate the observation that MCSD is a measure of defensiveness. If so, researcher and clinician alike can begin to work toward an important refinement of this very basic phenomenon of self-esteem.


Time factor as a prognostic indicator on the Bender-Gestalt test. Z-scores from Bender-Gestalt records of 62 psychiatric in- and outpatients were computed. The patients were put on active treatment, and a psychiatric rating was done to evaluate therapeutic recovery after a period of 19 to 24 months. These patients were grouped into three categories, i.e., patients with low Z-scores and good therapeutic recovery; patients with high Z-scores and poor therapeutic recovery; patients with low Z-scores who still showed poor response to treatment. Total time taken by each patient to reprduce the BG designs was noted as well. Results indicate that Z-score was a significant prognostic indicator for schizophrenics, affective psychotics, organic psychotics and patients who were suffering from anxiety and conversion reactions, but not for obsessive compulsive neurotics. Time taken for BG reproductions proved to be an important prognostic indicator, as patients who did not respond satisfactorily to the treatment took significantly more time than those who responded well to treatment. Hence, time taken by the patients for BG reproductions also needs to be considered when any predictions are made with regard to therapeutic outcome.


Extramarital relationships: a reapraisal. Extramarital relationships may not always disturb marital interaction, but on the contrary, marriages may have more permanence because many individuals find an answer to unmet needs in such relationships. In addition, a factor that rarely is mentioned in our society, which has vestiges of a puritanical ethic, is that an extramarital relationship can be an enjoyable experience for the individuals in and of itself. In a democratic society, which values choice, perhaps extramarital relationships can be undertaken if the individuals involved recognize: (1) the consequences of discovery, which may result in a threat to the solidarity of the marriage(s) involved; (2) the possibility that individuals so involved ultimately may wish to leave their own mates; and (3) that such a relationship may strengthen, not destroy, the unity of marriage.


Impact of therapist interventions on early sessions of group therapy. A methodology for studying the effect of therapist style on the process of group therapy was developed and applied to two early sessions in two different therapy groups. In general, the results supported the hypothesis that individually directed, confrontative interventions would lead to emotionally focused and immediate client responding. The results failed to confirm the hypothesis that relatively interpretive interventions would be the most productive. In fact, simple facilitations tended to produce higher mean focusing than all other types of interventions. Perhaps in very early sessions the most productive interventions are those designed simply to encourage patients to talk and interact. The decisive technique of a group therapist may be interpretation, which helps patients to comprehend the significance of personally relevant, emotionally charged interactions. However, in the fledgling group, the therapist may be most helpful by simply facilitating members' speaking up and interacting. Indeed, clarifications and interpretations may turn out to be the group therapist's most productive interventions, but perhaps not until after group cohesiveness is developed in early sessions.


The use of psychotherapy with dying patients: an exploratory study. A group of terminally ill patients received six sessions of psychotherapy. A second group of six patients served as nontreated controls. At the conclusion of the treatment sessions, psychotherapy patients reported a significantly greater increase in death-related anxiety and in their perceived purpose in life than did the nontreated group. Clinical impressions indicated that patients who participated in psychotherapy perceived their treatment experience positively and appreciated the opportunity to explore their feelings about imminent death. These results offer considerable support for the usefulness of psychotherapy to help such patients cope with the emotional stress generated by their limited life expectancy.


Neurotic verbalizations: an exploration of a Gestalt therapy assumption. Based upon assumptions of Gestalt Therapy, an objective, reliable scoring system for analyzing a specific aspect of verbal behavior, avoidant verbalization, was developed. Verbal samples were elicited from 30 female college students who previously had been rank ordered on the Neuroticism scale of the Maudsley Personality Inventory. The expectation that psychologically stressful questions would result in a significantly greater number of avoidant verbalizations was confirmed. Contrary to another expectation, no significant correlation between neuroticism as measured by the MPI and total number of avoidant verbalizations uttered by the Ss was found. Results were interpreted on the basis of the Gestalt Therapy theory of personality.


The stimulation of insight and self-awareness through body-movement exercise. The results suggest that even a brief body-movement experience can enhance understanding and self-awareness among normal students in a classroom setting. It may be that exercises could be used in other areas of learning or exploration. These completely noverbal activities may have the same beneficial effects as role-playing, with respect to increasing insight and self-awareness.


Prediction of homicide with the Rorschach. The use of a step-wise multiple discriminant analysis indicated that the Rorschach variables of S, A, P and m could discriminate with a good degree of accuracy the Rorschach protocols of murderers from those of nonmurderers. These empirically derived linear discriminant functions may serve as a useful adjunct to the clinical interpretation of Rorschach protocols in a criminal population.


The Holtzman Inkblot Technique in the assessment of organic brain damage. HIT performance of Barnes and Velez-Diaz' brain-damaged and control samples was contrasted. The results indicated good discrimination between Barnes' controls and both brain-damaged samples, but a poorer discrimination between Velez-Diaz' controls and the brain-damaged samples. This latter finding was attributed to the small N in Velez-Diaz' controls. Velez-Diaz' brain-damaged Ss showed the greatest degree of impairment of all samples, but Velez-Diaz' aged controls showed a poorer level of performance than did Barnes' brain-damaged patients.


Alcoholism and alienation.

Alcoholism and alienation. Dean's alienation scale was administered to comparable samples of alcoholic and normal males and females in groups of 10 to 12. Alcoholics not only evidenced significantly greater overall alienation than normals, but also demonstrated greater specific alienation, namely social isolation, powerlessness and normlessness. Furthermore, the degree of specific alienation reported by alcoholics, but not by normals, differed significantly. Social isolation reflected the greatest amount of alienation, then powerlessness, and normlessness revealed the least. Sex differences were found for the alcoholic sample, but not for the normals in terms of the specific forms of alienation, although not for overall alienation. In each of these instances, males expressed more alienation than females. The meaning of the results was discussed in view of both the etiology and treatment of alcoholism.


Neuroanatomical basis of binaural phase-difference analysis for sound localization: a comparative study. Four varieties of mammals whose medial superior olives range from large to none at all were tested for their ability to localize single, brief tone pips at various frequencies. Although each animal could localize high-frequency tone pips, their ability to localize middle- and low-frequency tone pips corresponded to the size of their medial superior olive (MSO). Since this latter range of frequencies is the one in which binaural phase-difference cues predominate, this anatomical-behavioral correspondence supports the idea that MSO is the chief binaural time-analyzing center for sound localization.