Limits to progressive reduction of resident skin bacteria by disinfection. Antiseptic preparations used repeatedly to disinfect the skin caused a reduction in yield of resident flora to a low equilibrium level beyond which further reduction did not occur. This equilibrium varied with the antiseptic preparation used. In a comparison of three preparations, the lowest equilibrium level was obtained with 95% ethyl alcohol. The further reduction in yield of skin bacteria on using alcohol after repeated disinfection with an antiseptic detergent preparation ('two-phase' disinfection) was not paralleled by a further reduction when the preparations used in the two phases were reversed, the antiseptic detergent being used after repeated disinfection with ethyl alcohol. 'Two-phase' disinfection was therefore seen to be due to further reduction in skin bacteria from the low equilibrium obtained with the antiseptic detergent to the lower level obtainable by alcohol disinfection. When repeated disinfection to equilibrium with alcohol was followed by a 'second-phase' handwash with a non-antiseptic detergent and water, there was a large increase in the yield of skin bacteria. This finding supports the view that a low equilibrium level occurs when the reduction in density of accessible bacteria through disinfection is balanced by the emergence of bacteria drawn from deeper layers of the skin.


Classification of staphylococci isolated from ocular tissues. A total of 145 strains of Gram-positive, catalase-positive, coagulase-positive, and coagulase-negative cocci isolated from the human eye were classified by the system of Baird-Parker (1966). These belonged to subgroups I, II, IV, and V and showed a wide range of antibiotic sensitivities unrelated to subgroup and category of lesion. The role of coagulase-negative staphylococci in producing postoperative ocular infections has been especially emphasised.


Abnormal retino-geniculate and geniculo-cortical pathways in several genetically distinct color phases of the mink (Mustela vison). Several genetically distinct color phases of mink, which all show an abnormal reduction of pigment in the retinal pigment epithelium and which also show abnormalities of the retinofugal pathways, have been studied. Autoradiographic methods have been used to demonstrate the retino-geniculate pathways, and retrograde degeneration or the retrograde transport of horseradish peroxidase has been used for the geniculo-cortical pathways. The retino-geniculate abnormality is mild in some of the color phases and extremely severe in others, but within any one color phase the variability is relatively low. Although the severity of the abnormality varies between color phases, a rather specific pattern of abnormal geniculate innervation is recognizable for mink in general and this is distinct from that found in Siamese cats. In the abnormal mink the size of geniculate lamina A1 is reduced and there is an abnormal crossed input going to the intermediate sectors of this reduced layer. Layer C1 also receives an abnormal crossed input, but this is more variable than that going to A1 and there appears to be little correspondence, retinotopically, between the normal inputs to layers A1 and C1. In some of the abnormal mink there are interruptions within the cytoarchitectionically definable layer A1, and opposite these gaps reduplications of layer A are commonly seen, as though there is an intrinsic geniculate mechanism for generating the characteristic multilaminar geniculate structure. However, there are also numerous examples of fusions between layers receiving afferents from the same eye, and these demonstrate that the development of geniculate lamination must also be under the influence of the retinal inputs. The geniculo-cortical pathway shows a normal topography in most of the mink. Abnormal geniculo-cortical projections, comparable to the "Boston" pattern of Siamese cats are extremely rare, and their occurrence could not be correlated with the severity of the retino-geniculate abnormality or with the laminar pattern in the lateral geniculate nucleus. We suggest that the development of one or the other pattern of geniculo-cortical projection may depend upon the relative timing of the two mechanisms that produce the geniculate lamination.


Retinotopic organization of areas 18 and 19 in the cat. The location and retinotopic organization of areas 18 and 19 in cat cortex were determined using electrophysiological mapping techniques. These two areas each contain a single representation of the visual hemifield and each has a distinctive cytoarchitecture. The visual hemifield representations in these two areas are nearly mirror images of each other. Compared to area 17, areas 18 and 19 have less cortical surface area, have a lower cortical magnification factor, contain less of the visual field and contain second order instead of first order transformations of the visual hemifield. An unusual asymmetry was found between the representations of the upper and lower visual quadrants not seen before in maps of other areas of cat or other species. A considerable amount of variability in the retinotopic organization of these two areas was found among cats.


Thalamic relay nuclei for cerebellar and certain related fiber systems in the cat. Anterograde labeling techniques were used to define the terminal distributions in the thalamus of afferents arising in the deep cerebellar nuclei, entopeduncular nucleus and substantia nigra. Anterograde and retrograde labeling methods were then used to determine the extent of the cortical projections of the cerebellar relay nuclei. The cerebellar projection to the contralateral ventral nuclei of the thalamus terminates in a zone which is separate from that receiving pallido- and nigrothalamic fibers. None of the zones of termination of these fiber systems corresponds to commonly recognized cytoarchitectonic divisions. Instead, they include parts of the ventroanterior (VA), ventrolateral (VL) and principal ventromedial (VMp) nuclei. Some cells within the zone of termination of cerebellar afferents project to parietal cortex (areas 5 and 7). A further, distinct group of cells in this zone projects to motor cortex (area 4). But projections to area 4 also arise from small groups of cells: (a) in the zone receiving nigro- and pallidothalamic fibers; (b) in the part of VL, distinct from the cerebellar terminal zone, in which spinothalamic fibers terminate. Cerebellar, nigral, and entopeduncular fibers also terminate in the intralaminar nuclei. These projections are far greater in extent than those arising in the spinal cord. Some parts of the intralaminar nuclei are dominated by a particular afferent system, while others show substantial overlap of the terminal zone of several afferent systems.


Functional organization of rat olfactory bulb analysed by the 2-deoxyglucose method. The spatial patterns of activity elicited in the rat olfactory bulb under different odor conditions have been analysed using the 2-deoxyglucose (2DG) technique. Rats were injected with 14C-2DG, exposed to controlled environments of amyl acetate, camphor, cage air, dimethyl disulfide, and pure air and autoradiographs prepared by the method of Sokoloff. Amyl acetate was associated with regions of glomerular layer densities in the anterolateral and mid- to posteromedial parts of the bulbar circumference, as previously reported. The extents of the densities increased with increasing concentration. Camphor odor was associated with regions of increased density in the anterodorsal and mid- to posteromedial parts of the bulb. Exposure to cage air produced scattered densities in the posteromedial and posterolateral bulb. Exposure to dimethyl disulfide gave variable results. Pure air was associated with a minimal number of small dense foci. The results with amyl acetate, camphor and cage air suggest that patterns for different odors are distinguishably different but overlapping. The regions of activity are greatest in extent and density with the highest odor concentrations. These define the regions within which more restricted and isolated foci appear at lower concentrations. The results thus provide evidence for the specific role of spatial factors in the neural processing of odor quality and odor concentration.


An HRP and autoradiographic study of the projection from the cerebellar cortex to the nucleus interpositus anterior and nucleus interpositus posterior of the cat. The recently developed anatomical techniques of retrograde transport of the enzyme horseradish peroxidase (HRP), anterograde transport of tritiated amino acid, and intracellular injections of HRP were used to study the organization of the corticonuclear projection to the nucleus interpositus anterior (NIA) and the nucleus interpositus posterior (NIP) of the cat. Injections of HRP into the NIA and the NIP revealed that the major areas of the cortex which provided afferents to these two nuclei were the intermediate cortex of the anterior lobe (IAL) and the paramedian lobule (PML). There were, however, significant differences in the distribution of Purkinje (Pk) cells which projected to each nucleus. The NIA received afferents from all areas of the IAL while the NIP projection area was restricted to a band located at the medi-almost aspect of the lobe. All areas of the PML, in particular the intermediate folia, projected to the NIP, while the Pk cells which sent axons to the NIA were restricted to the rostral and caudal folia of this lobule. The projection from each area was somatotopically organized. The axons of intracellularly stained Pk cells were followed to their termination in the NIA and NIP confirming the results obtained with the two extracellular techniques. An attempt was made to examine the organization of the corticonuclear projection at the single cell level in the PML. Pk cells located in the same sagittal plane appeared to terminate in the same area of the same nucleus while Pk cells located not more than 500 micrometers medial or lateral to each other terminated in different nuclei. Basically, the organization of the corticonuclear projection from the IAL is longitudinally organized while the PML has a much more complex arrangement in which the Pk cells projecting to the NIA and NIP are interspersed.


Reexamination of the dorsal root projection to the spinal dorsal horn including observations on the differential termination of coarse and fine fibers. Primary afferent fibers in the lumbar, sacral, and caudal spinal segments of several mammals (rat, cat, monkey) were stained by applying horseradish peroxidase to the proximal part of cut dorsal rootlets and reacting the tissue histochemically after several hours of survival. The stained fibers' pattern of termination in the dorsal horn was similar in all three species, with many bouton-like enlargements in the ipsilateral marginal zone, substantia gelatinosa, and nucleus proprius, as well as a few projections at each level to the dorsal commissure and contralaterally to the ventral border of the nucleus proprius. Partial lesions of dorsal rootlets in monkey revealed that the thin fibers comprising the lateral division end principally in the marginal zone and substantial gelatinosa, while the thick fibers of the medial division terminate in the nucleus proprius and deeper regions, contributing little to the substantia gelatinosa and marginal zone. On the basis of the termination patterns observed for whole and partly sectioned rootlets, the superficial dorsal horn can be divided into at least four regions. (1) The marginal zone (lamina I of cat) appears to receive terminations from intermediate (smaller myelinated) fibers; (2) the outer substantia gelatinosa (outer lamina II) receives many terminations from the very finest afferent fibers; (3) the inner substantia gelatinosa (inner lamina II) receives endings from some of the finest fibers and also from intermediate (smaller myelinated) fibers; and (4) the superficial part of the nucleus proprius (lamina III) receives endings from intermediate and large diameter dorsal root fibers.


Morphological features of functionally defined neurons in the marginal zone and substantia gelatinosa of the spinal dorsal horn. Functional characteristics of spinal neurons located in the marginal zone (lamina I) and substantia gelatinosa (lamina II) were compared to their structural features by intrcellularly staining the source of unitary potentials with horseradish peroxidase (HRP) in unanesthetized, spinal cats. The responses of postsynaptic units to graded electrical volleys in intact dorsal roots and to physiological stimulation revealed that the peripheral excitatory input to neurons of the region is dominated by slowly conducting afferent fibers; often, the input to a given element is largely from a particular class of receptors. One type commonly seen received its principal peripheral excitation from low threshold mechanoreceptors with A delta or C afferent fibers. Mechanoreceptive elements often exhibited a marked, prolonged habituation and many were not excited by afferent volleys. Other units were predominantly excited by nociceptors with myelinated or unmyelinated fibers, or by thermoreceptors with unmyelinated fibers. A few units (principally the thermoreceptive) showed substantial ongoing activity which was modulated by sensory stimulation, but most had little or none. The HRP staining revealed neuronal morphology in fine detail. No relationship between neuronal configuration and physiological response was discerned. Soma location was not always linked to afferent input, although the cell bodies of nociceptive and thermoreceptive neurons tended to be in lamina I or outer lamina II (SGo) while those of the innocuous mechanoreceptive meurons tended to be in inner lamina II (SGi). The locus of a neuron's major dendritic arborization was more closely related to the source(s) of peripheral excitation. Cells excited by nociceptors with myelinated fibers had major dendritic projections in the marginal zone. Cells excited by nociceptors or thermoreceptors with unmyelinated fibers had important dendritic branching in the SGo. Innocuous mechanoreceptive neurons had primary dendritic arborization in the SGi when the input derived from unmyelinated fibers, or in the SGi and extending into the outer nucleus proprius (lamina III) when the afferent drive came from A delta fibers. These findings support the concept that laminae I and II constitute a major termination region for thin primary afferent fibers, myelinated fibers from nociceptors ending principally in lamina I and unmyelinated fibers from nociceptors, thermoreceptors, and mechanoreceptros terminating predominantly in lamina II. Substantial integrative and distributive functions can be expected of such an afferent termination zone.


Organization and development of brain stem auditory nuclei of the chicken: dendritic gradients in nucleus laminaris. Nucleus laminaris (NL) is a third-order auditory nucleus in the avian brain stem which receives spatially-segregated binaural inputs from the second-order magnocellular nuclei. The organization of dendritic structure in NL was examined in Golgi-impregnated brains from hatchling chickens. Quantitative analyses of dendritic size and number were made from camera lucida drawings of 135 neurons sampled from throughout the nucleus. The most significant results of this study may be summarized as follows: (1) The preponderant neuron in n. laminaris may be characterized as having a cylindrical-to-ovoid cell body, about 20 micrometer in diameter. The neurons comprising NL were found to be nearly completely homogeneous in issuing their dendrites in a bipolar fashion: one group of dendrites is clustered on the dorsal surface of the cells, the other group on the ventral. The dendrites of NL are contained within the glia-free neuropil surrounding the nucleus. From the rostromedial to the caudolateral poles of NL there is a gradient of increasing extension of the dendrites, increasing number of tertiary and higher-order dendrites, and increasing distance from the somata of the occurrence of branching. (2) The total dendritic size (sum of the dorsal) and ventral dendritic lengths of the cells) increases 3-fold from the rostromedial to the caudolateral poles of NL. About 50% of the variance in dendritic size is accounted for by the position of the cells in NL, and the gradient of dendritic size increase has the same orientation across NL as the tonotopic gradient of decreasing characteristic frequency in NL. (3) From the rostromedial pole to the caudolateral pole of NL there is an 11-fold decrease in the number of primary dendrites along a gradient coinciding with the length and frequency gradients. Sixty-six percent of the variance in dendrite number is accounted for by position in the nucleus. (4) The correlation of dorsal and ventral dendritic size on a cell-by-cell basis is not high (r = 0.47), indicating a fair amount of variability on the single-cell level. On the other hand, the average dorsal dendritic length within an isofrequency band in NL correlates very highly with the average ventral dendritic length. Thus, on an areal basis, the amount of dendritic surface area offered to the dorsal and ventral afferents is tightly regulated. (5) The dorsal and ventral dendrites have separate gradients of increasing length and number across NL. The dorsal gradients are skewed toward the rostrocaudal axis, while the ventral dendritic gradients are skewed mediolaterally. (6) There was no correlation between either dendritic size or number of primary dendrites and the size of the somata in NL, which remains relatively constant throughout the nucleus. Several hypotheses about the ontogenetic control of dendritic structure are examined in light of the above data. Of these, the hypotheses that the ontogeny of dendritic size and number is largely under afferent control receives a great deal of circumstantial support.


Development of abnormal recrossing retinotectal projections after superior colliculus lesions in newborn Syrian hamsters. The development of the retinal projections to the roof of the midbrain was studied in Syrian hamsters after right superior colliculus (SC) lesions on the day of birth, using both autoradiographic and degeneration techniques. The dead tissue resulting from the heat lesion is not completely removed until the eighth day after birth. Normally the midline of the SC is defined by a pia-lined fissure separating the left and right colliculi, but in the animals with early unilateral lesions, the pia at the midline is damaged. When it regrows, together with vascular and other meningeal tissues, it forms a flat tissue bridge across the midline as early as two days after the lesion. When the axons from the left eye reach the right SC, they encounter the dead tissue and separate into two bundles. One bundle courses over the surface of the dead tissue and one grows underneath it. It is not until the third to fourth day that axons in the dorsal bundle cross the midline, via the tissue bridge, to terminate anomalously in the medial wall of the left SC. When the quantity of such recrossing axons is small, they overlap extensively with the optic tract fibers from the other (right) eye which normally have innervated the entire SC by day 3. However, it appears that as the density of the recrossing axons increases they displace the axons originating in the other eye from the medial wall of the left SC. Thus, eventually fibers from both eyes terminate in the left SC, occupying separate territories with little, if any, overlap. Axons in the ventral bundle begin to innervate the deep layers of the right SC on day 2. These axons were never observed to recross the midline. These results indicate that mechanical guidance and axonal segregation dependent on relative densities are two processes that govern the development of retinotectal projections after early SC lesions in hamsters.


Alpha- and gamma-motoneurons in the adult human spinal cord and somatic cranial nerve nuclei: the significance of dendroachitectonics studied by the Golgi method. A modified Golgi method (Vaisamruat and Hess, '53) was found to give satisfactory impregnation of cell bodies and dendrites, but not of dendritic spines and axons, in adult human material fixed by immersion in formalin. Examination of the motor columns in the spinal cord intumescences and of the third and twelfth cranial nerve nuclei revealed four neuron types, based on dendritic field size and dendritic branching pattern. Two of these were recognized as alpha-motoneurons; one of them was seen only in the medial motor column of the spinal ventral horn, while the other was observed in the cranial motor nuclei as well as the spinal lateral motor column. Differences in somadendritic dimensions in this neuron type were thought to reflect motor unit size, and thus terminal axon field dimensions. Of the two types of gamma-motoneurons recognized in the spinal cord and oculomotor nucleus, one was a miniature version of the commoner type of alpha-motoneuron. On this basis, it is proposed that it may give rise to fusimotor axons with plate endings. The second type of gamma-motoneuron does not resemble any of the other motoneuron types, and its axons may therefore be thought to terminate in trail endings.


Topographic organization of the projections of the retina to the pretectal region in the rat. The pattern of projection of the retina to the pretectal region and its retinotopic organization were investigated in the rat by autoradiographic and silver impregnation techniques for axonal pathways. The endings of retinal axons form three terminal fields in the pretectum in: 1, olivary pretectal nucleus (PO), bilaterally; 2, posterior pretectal nucleus (PP), bilaterally; and 3, nucleus of the optic tract (NTO), contralaterally. The following retinotopic pattern was observed in rats surviving peripheral retinal lesions and injections of 3H-proline in the same eye, when the positions occupied by terminal degeneration in Fink-Heimer stained sections were matched with the corresponding areas deficient in radiolabel in adjacent autoradiographic sections showing the surviving parts of the terminal fields. The nasal periphery of the retina maps along the adjoining edges of PO and PP, both of which extend obliquely, in a posterolateral direction, through the entire extent of the pretectum. Both nuclei map the line of representation of the anterior midline (in the temporal retina) along their opposite edges (anterolaterally, in PO; posteromedially, in PP). This mirror-image symmetry is completed by the representation of the ventral peripheral retina separately in the rostral poles and the dorsal peripheral retina separately in the caudal poles of both nuclei. The map in NTO is vertically oriented, with the temporal retina, dorsally, the nasal retina, ventrally, the ventral retina, rostrally, and the dorsal retina caudally represented. The binocular area of the terminal field in PO is subdivided by a terminal-free zone into two parts that may process separately events in the central and lateral visual field.


Excess DNA in the nuclei of the subesophageal region of octopus brain. Cytophotometric analyses of Feulgen-stained nuclei present in homogenates of vertical and subesophageal lobes of octopus brain have shown that the latter region contains larger nuclei with up to several times the amount of DNA present in vertical nuclei. No obvious relationship was found between DNA content and nuclear size. Except for a rather small minority, nuclei of the vertical lobe have a uniform size and the expected diploid amount of DNA. These parameters are not substantially dependent on body weight. In contrast, the DNA content of subesophageal nuclei increases progressively with body weight. The amount of DNA found in subesophageal nuclei does not seem to be a simple multiple of the diploid or haploid value.


A case of behavioral heterosis in mice: quantitative and qualitative aspects of performance in a water-escape task. Fourteen hundred and four mice from two inbred strains (Balb/c and C57BL/6), F1, F2, and backcrosses were subjected to four trials in a water-escape task and to a swimming test. Detailed analysis of mice behavior in these situations showed that the "F1 hybrid vigor" affected behavioral characters not directly related to physical vigor but of potential adaptive value. Their superiority was mainly due to more frequent adoption of an efficient behavioral tactic (direct or edge escape paths toward the exit) and more rapid progress with experience in this respect than other generations exhibited. These results clearly show that heterosis is not limited to physical vigor but may extend to behavioral and even psychological characters.


Decreased locomotor and investigatory exploration after denervation of catecholamine terminal fields in the forebrain of rats. Exploratory behaviors were examined after bilateral microinjections of 6-hydroxydopamine into two hypothalamic sites that produced different patterns of denervation of forebrain catecholamine terminal fields. After anterolateral injections rats locomoted and reared less in a novel open field, responded abnormally to changes in the degree of novelty of the open field, and investigated a novel object less. These are deficits in exploratory behavior because they were not secondary to the inhibition of open-field behavior by hyperemotionality, by general motor disability, or by the failure to detect novel spaces or objects. Such anterolateral injections produced loss of catecholamine fibers, determined histochemically, in neocortical, hippocampal, anterolateral hypothalamic, mesolimbic, mesocortical, and anteromedioventral striatal terminal fields and loss of dopaminergic perikarya in the A10 and anteromedial A9 cell groups. No deficits in exploratory behaviors occurred, however, after bilateral anteromedial 6-hydroxydopamine injections that denervated neocortical, hippocampal, and anteromedial hypothalamic catecholamine terminal fields. A critical forebrain catecholaminergic innervation for exploratory responses to novel stimuli may be within areas that were denervated by anterolateral but not by anteromedial hypothalamic 6-nydroxydopamine injections. These areas are mesolimbic, mesocortical, anteromedioventral, and anterolateral hypothalamic terminal fields.


L-Dopa repairs deficits in locomotor and investigatory exploration produced by denervation of catecholamine terminal fields in the forebrain of rats. In a previous study rats were shown to have decreased locomotor and investigatory exploration after bilateral microinjections of 6-hydroxydopamine into the anterolateral hypothalamus. These deficits correlate with the loss of catecholamine terminals in neocortical, limbic, and anteromedioventral striatal brain sites. To test whether this correlation was causal, central catecholamines were increased by the intraperitoneal administration of L-3,4-dihydroxyphenylalanine (L-dopa), 10--40 mg/kg) after inhibition of extracerebral L-amino acid decarboxylase. Such treatment repaired the deficits in locomotor exploration and investigation in 6-hydroxydopamine rats. Pretreatment with the catecholamine antagonist chlorpromazine (1--2 mg/kg) blocked the increase in locomotor exploration and investigation produced by L-dopa in 6-hydroxydopamine rats. The results suggest, but do not prove, that L-dopa produced these behavioral effects by increasing central catecholamines at the denervated catecholamine receptor sites in the forebrain. These data and the data from the previous study are complementary evidence for the hypothesis that forebrain catecholamine synaptic action is necessary for normal exploratory behavior.


Transfer of dietary selenium to milk.

Transfer of dietary selenium to milk. Twenty-five lactating cows were used in groups of five to study the amounts of dietary selenium transferred to milk. Amounts of dietary selenium varied from deficient to five times the adequate concentration and ranged between 41 ppb and 828 ppb. Sodium selenite and brewers grains, a rich naturally occurring source of selenium, supplied supplemental selenium. Selenium in milk and plasma were related to the amount consumed, but the response was nonlinear since 4.8% of the added selenium was transferred to milk with a deficient diet but only .9% of the amount of added selenium was in milk of cows consuming diets adequate in selenium. Nineteen percent of the selenium furnished in brewers grains appeared in the milk when the ration was deficient in selenium. The small amounts of selenite selenium transferred from the diet to milk were too little (5.5 micrograms/kg) to be a potential hazard to human health when a diet containing .1 to .2 ppm of selenium was fed to dairy cows, an amount sufficient to meet the cow's dietary needs.


Effect of low and high ambient temperatures on metabolism of radioiodine by the lactating goat. Two lactating goats given an oral dose of iodine-125 excreted 16.8, 52.5, and 9.8% of the dose into the milk, urine, and feces when the ambient temperature was 33 C. The thyroids contained about .7% of the oral dose. At 5 C only 2.6% of the radioiodine was in the milk, but 71.2% was in the urine, 18.1% in the feces, and about 10% in the thyroid. The reduced competition by the thyroid plus enhanced transfer of radioiodine from blood to milk at 33 C was responsible for the large transfer of radioiodine into milk at 33 C.


Hypothyroidism and anemia related to fluoride in dairy cattle. Blood and urine were collected from 72 cows in six dairy herds with varying severity of dental and bone fluorotic lesions. Urinary fluoride averaged 5.13 ppm and ranged from 1.04 to 15.7 ppm fluoride. Thyroxine and triiodothyronine in serum decreased with increasing urinary fluoride, eosinophils increased, and cholesterol tended to decrease. Cattle afflicted with fluorosis developed hypothyroidism, anemia, and eosinophilia of leukocytes. Bone ash averaged 2400 ppm fluoride in 22 specimens from eight herds (range 850 to 6935 ppm fluoride). Mineral supplements were the main sources of excess fluoride. Fluoride lesions were on some cows of all herds suggesting that fluoride may affect the health and performance of some cows in "normal" herds. Fluoride lesions were on young cattle and calves in fluorosis herds.


Microbial transaminase activities and their relationship with bovine rumen metabolites. Two each adult male crossbred cattle and murrah buffalo were fed a diet of alfalfa hay, chopped wheat straw, and concentrate mixture. Total rumen transaminase activity of cattle was higher than that of buffalo. Rumen protozoal fractions showed higher total transaminase activity than bacterial fractions in both ruminant species. Besides generally studied glutamate oxalacetate transaminase and glutamate pyruvate transaminase, a large number of other microbial transaminases also have been detected in the rumen of both the ruminant species. Bacterial fractions of rumen liquor were devoid of transaminases utilizing tryptophan, threonine, and lysine as their substrates. Ruminal ammonia and nonprotein nitrogen were correlated positively with microbial transaminases in both species. Transamination reactions may be important for assimilation of ruminal ammonia to cellular proteins.


Subjective loudness and annoyance of filtered N-wave sonic booms. The contribution of the "infrasonic" low-frequency content of sonic boom N waves to subjective loudness and annoyance has been investigated. An extended low-frequency response loudspeaker-driven simulation booth was employed, with computer-generated input test signals. For test N waves of 1 ms rise time and 150 ms duration, frequencies below 25 and 50 Hz, respectively, were cut off by digital filters simulating simple RC circuits. The filtered signal amplitude was adjusted versus the amplitude (48 Pa) of a reference unfiltered N wave (effective low-frequency cutoff approximately 0.1 Hz) until the two sounded equally loud (first experiment) or equally annoying (second experiment). The amplitude differences for equality were very slight: less than 0.6 dB at most. Surprisingly, while loss of the low frequencies slightly decreased the loudness, it slightly increased the annoyance.


Normal short-latency electrophysiological filtered click responses recorded from vertex and external auditory meatus. We recorded normal electrophysiological responses to third-octave filtered clicks from external auditory meatus (EAM) and vertex electrodes referred to coupled earlobe electrodes (forehead ground). From both vertex and EAM, polarity-sensitive responses predominated at low frequencies and exhibited characteristics of both phase-locked neural responses (frequency-following response or FFR) and cochlear microphonics (CM). The FFR-like response predominated at the vertex site and the CM-like response predominated at EAM. At high frequencies, polarity-insensitive responses closely resembled rectangular-pulse click action potentials and brainstem evoked potentials, with clearly defined N1 and V peaks recorded from EAM and vertex, respectively. As frequency was lowered, the N1 and V peak latencies increased, the peaks broadened, and the latency-intensity curves steepened with greater prolongation occurring at lower click intensities. Lowering click frequency also shortened the N1-V interval and caused the plot of N1-V interval versus click intensity to become steeper. Plots of polarity-insensitive response amplitudes and thresholds against frequency revealed a high frequency bias for both N1 and V, but the V "frequency response" was flatter. A possible explanation of the shortened N1-V interval at low click frequencies based on this flatter V "Frequency response" is presented.


Stop consonant place perception with single-formant stimuli: evidence for the role of the front-cavity resonance. The third formant and the second formant were found on average to cue the place of articulation of intervocalic stop consonants equally well when the stop consonants occurred before the vowel/i/. This result and others provide some support for the notion that the fundamental resonance of the front cavity plays an important role in the perception of the phonetic dimension of place of articulation.


Selective adaptation effects in infant speech perception paradigms. The present studies were conducted to explore the possible role of selective adaptation in infant speech perception. In the first study, in which the growth of adaptation was examined by presenting adult listeners with successive blocks of 20 repeating stimuli, reliable adaptation effects were observed after only 80 stimulus presentations. In addition, recovery following adaptation was relatively rapid and complete by the end of a postadaptation identification sequence. Experiment II constituted a more direct investigation of the possible role of adaptation in infant speech perception in which actual protocols from heart-rate (HR) and non-nutritive high-amplitude sucking (HAS) infant testing sessions were presented to adult listeners. Reliable adaptation effects were obtained within the HAS protocol, but not within the HR format. This pattern of results was consistent with that observed in experiment I. The implications of these adult adaptation results for the processes underlying the infant's responsiveness to these stimulis were discussed.


Stop consonant discrimination based on human audition. A system for discrimination of stop consonants has been designed on the basis of studies of auditory physiology and psychophysics. The system consists of a one-third octave filter bank as an approximation to auditory tuning curves, a bank of high speed, wide dynamic range envelope detectors, a logarithmic amplifier, and a digital computer for analysis and display. Features, chosen on the basis of psychophysical experiments, are then abstracted, and fed to a discriminant analysis program which decides on the most probable phomene. Discrimination accuracy of about 77% for stop consonants in initial position has been achieved, with a 15-speaker data set.


Investigating the MESA (multipoint electrotactile speech aid): the transmission of connected discourse. Three normal-hearing young adults were tested in their reception of connected discourse materials under two receiving conditions; visual-reception alone (lipreading) and visual reception in conjunction with an electrotactile speech aid (MESA). Subjects were artificially deafened with earplugs and white noise; all stimuli were delivered live-voice using a special tracking procedure. Analysis of the data suggests that after an initial period of learning, combined visual and electrotactile receptive performance exceeds lipreading-alone performance. After extensive learning however, performance in lipreading alone or MESA plus lipreading is practically equivalent.


Fatigue and recovery of the human acoustic stapedius reflex in industrial noise. The fatiguability of the acoustic stapedius muscle reflex in an actual noisy industrial environment was investigated in normal-hearing subjects. In a laboratory situation a small depression was found with a considerable individual variability. The stapedius reflex recovered slowly, approximately as a linear function of time. In a field study on an entire day of exposure in a ship-building yard the reflex depression was on the average 4 dB in response to a stimulation of 2000 Hz 10 min after the end of the workday. This corresponds to less than 8 dB immediately at the end of the exposure.


Comparison of WKB and finite difference calculations for a two-dimensional cochlear model. There are many points of uncertainty in the subject of cochlear models. In this paper only the question of efficient computing methods is addressed. For the cochlear model with a one-dimensional approximation for the fluid motion, Zweig, Lipes, and Pierce [J. Acoust. Soc. Am. 59, 975-982 (1976)] have shown that the WKB method agrees well with a direct numerical integration. For the two-dimensional fluid model, Neely [E.D. thesis, California Institute of Technology, Pasadena, CA (1977)] has shown that a direct finite difference solution is an order of magnitude faster than the integral equation approach used by Allen [J. Acoust. Soc. Am 61, 110-119 (1977)]. In the present work, a formal WKB solution is derived following Whitham [Linear and Nonlinear Waves (Wiley, New York, 1974)]. The advantage of this formulation is simplicity, but the disadvantage is that no error estimate is available. We find that the numerical results from the WKB solution agree well with those of Neely (1977), while the computer time is reduced by another order of magnitude. Thus, the WKB method seems to offer the satisfactory accuracy, efficiency, and flexibility for treating the more realistic cochlear models.


Comparison of WKB calculations and experimental results for three-dimensional cochlear models. The WKB asymptotic method is applied to the calculation of cochlear models with square scala cross section, for which the fluid motion is fully three dimensional. The analysis begins with the exact solution for wave propagation in a duct with constant properties. This solution is somewhat tedious but straightforward, since it requires a Fourier series expansion across the duct. Then with the formulation of Whitham [Linear and Nonlinear Waves (Wiley, New York, 1974)], the approximate solution is readily generated for the duct with properties which vary slowly along the length. Numerical calculations are carried out for the experimental models of Cannel [Ph.D. thesis, Univ. of Warwick (1969)] and Helle [Dr.-Ing. disser., Technische Univ., Müchen (1974)] who furnish quantitative details of both "basilar membrane" response and model parameters. Without any free parameters for adjusting, the present WKB solution shows quite satisfactory agreement with the experimental model results. Computer time is reasonable; the calculation of displacement envelope and phase at a number of stations along the cochlea for a given frequency requires only one second of CPU time. Thus the credibility and practically of the approach is established for the investigation of yet more realistic and more elaborate cochlear models.


Perceived sound quality of sound-reproducing systems. Perceived sound quality of loudspeakers, headphones, and hearing aids was investigated by multivariate techniques from experimental psychology with the purpose (a) to find out and interpret the meaning of relevant dimensions in perceived sound quality, (b) find out the positions of the investigated systems in these dimensions, (c) explore the relations between the perceptual dimensions and the physical characteristics of the systems, and (d) explore the relations between the perceptual dimensions and overall evaluations of the systems. The resulting dimensions were interpreted as "clearness/distinctness," "sharpness/hardness softness," "brightness-darkness," "fullness-thinness," "feeling of space," "nearness," "disturbing sounds," and "loudness." Their relations to physical variables were explored by studying the positions of the investigated systems in the respective dimensions. Their relations to overall evaluations were studied, and the implications of the investigations for continued research are discussed.


Role of distinctive features in dichotic perception of 21 English consonants. The role distinctive features played in the identification of 21 dichotically presented syllable initial English consonants was evaluated. Results were analyzed for the entire stimulus set and for various intra- and inter-manner class comparisons. Consistent with previous dichotic studies of stops alone, stops as a subgroup showed a large right ear advantage, a high incidence of blend errors, and greater accuracy in identification when the competing stimuli contrasted on one (rather than two) distinctive features. Results for manner classes other than stops, for inter-manner comparisons and for the total stimulus set indicated increased correct identification with increasing numbers of distinctive feature differences between the two syllables. Analysis of error patterns revealed that, in addition to the stops, continuants, and stop-continuant, stop-affricate, and stop-nasal pairs revealed significant numbers of blend errors. Error responses also showed a tendency for unmarked feature specifications to predominate significantly over marked feature specifications. Finally, the magnitude of the right ear advantage varied significantly as a function of manner class, but not as a function of number of feature contrasts.


Larger ear replica for KEMAR manikin.

Larger ear replica for KEMAR manikin. A larger external ear replica for use on a KEMAR manikin has been developed to meet the needs of hearing aid designers and other manikin users. The ear was selected from the 24 subject sample used by Burkhard and Sachs (1975) as the basis for the original ear development. Ear dimensions were chosen to be two standard deviations larger than the existing ear. A mirroring technique was used to give right and left ears with nearly identical properties. Responses of the manikin with the new ears are given and compared with responses with the original ears.


Mechanical properties of single motor units in speech musculature. Electromyograms were recorded from three subjects during steady, low-rate discharge of single motor units of anterior belly of digastric, accompanying maintenance of isometric tension. The overall tension developed in muscles subserving mandibular depression was recorded from a force tansducer situated inferior to the mandibular symphysis. The force transducer output from the 90 ms period immediately following each of the several hundred discharges of a motor unit was averaged to obtain a characteristic tension curve for the contractile response (twitch) of that unit. Peak twitch tensions and time taken to develop peak tension (contraction time) were recorded for 27 motor units. The obtained median twitch tension of 0.26 g and contraction time of 29 ms were compared with values obtained for units in other human muscles and in animal muscles. As expected from the size principle, later recruited units possessed higher twitch tensions. Contrary to some statements of the size principle, contraction times were not shorter in later recruited units. Data on muscles elevating the mandible were also evaluated.


Temporal summation of pure tones in birds. Two parakeets and two field sparrows were trained by a method of avoidance conditioning to respond to pure tones. Absolute thresholds were obtained for 10 durations of a 2.86-kHz tone. The temporal integration function for these four birds can best be described by a time constant pi of 230 ms. These data are similar to those reported for a number of other species.


Further studies on the Schroeder-Hall hair-cell model. The Schroeder-Hall hair-cell model [M.R. Shroeder and J.L. Hall, "Model for mechanical to neural transduction in the auditory receptor," J. Acoust. Soc. Am. 55, 1055-1060 (1974] was further explored using additional stimulus waveforms and analysis techniques. The model is shown to have other interesting properties such as the ability to generate realistic two-tone interactions. Amended to limit the growth of transmitter release, the model also produces realistic adaptation and incremental response data. Other amendments improved high-intensity period histogram waveshapes. No one amendment, however, allowed the model to produce both realistic adaptation curves as well as period histogram waveforms that faithfully mimicked physiological data.


Posterior subcapsular cataracts in steroid-requiring asthmatic children. Slit-lamp examinations were performed on 24 children and adolescents with severe asthma, all of whom had received steroids for at least 365 days. Posterior subcapsular cataracts (PSCC) were detected in 7 (29.1%). None of the patients had been treated with beclomethasone. All 7 of the patients with PSCC were in the subgroup of 14 patients who had been on the highest doses of corticosteroid, 10 mg or more per day, for the longest period of time. The 7 children with PSCC were all below the fifth percentile for height and had fallen away from their normal growth curve. Of the 17 children in whom PSCC were not detected, only 1 was below the fifth percentile for height. It would seem from our results that the steroid-requiring asthmatic who is growth-suppressed is at an increased risk for developing PSCC. We have documented the reversal of PSCC in 2 children. Both of these children had been placed on beclomethasone, which allowed for the discontinuation of daily prednisone in one case and a reduction to less than 10 mg per day of prednisone in the other. The reversal occurred within 6 months of starting on beclomethasone.


Fatal myocardial toxicity during continuous infusion intravenous isoproterenol therapy of asthma. We recently utilized continuous infusion intravenous isoproterenol in the treatment of respiratory failure in an 18-yr-old steroid-dependent asthmatic female. Aminophylline, hydrocortisone, aerosolized isoetharine, and oxygen were also administrered. The patient responded to this therapy, with PaCO2 falling from 70 torr to 33 torr in 18 hr. The maximum isoproterenol dosage administered was 0.32 microgram/kg/min. Thirty-six hours following the institution of therapy, while the isoproterenol was being tapered, the patient experienced an increase in respiratory distress followed by cardiac arrest. Postmortem examination revealed multiple small areas of myocardial necrosis. These findings, unusual in asthma, probably were related to the effects of isoproterenol or the combination of isoproterenol and aminophylline on the stressed myocardium. The vulnerability of the hypoxic myocardium to the effects of isoproterenol suggests that careful cardiac monitoring is essential in the management of patients receiving this medication for treatment of respiratory failure secondary to severe asthma.


Pneumomediastinum as a complication of asthma in teenage and young adult patients. The characteristics of 11 patients (age range, 13 to 31 yr) with asthma complicated by pneumomediastinum are presented. Nine of 11 patients were male. Cough and chest pain were prominent symptoms in the affected individuals. Subcutaneous emphysema was noted in all but 1 patient who presented with an unusual radiographic finding: a radiolucent rim surrounding the pulmonary artery. Hamman's sign was detected in 7 patients. None of the patients required specific therapy for pneumomediastinum. The pathophysiology of pneumomediastinum was reviewed.


Exercise-induced anaphylactic reaction to shellfish. The syndrome of immediate type I food hypersensitivity, mediated by tissue-bound IgE antibody and mast cell histamine release, is well recorded in the medical literature. This case study represents a previously undescribed late food hypersensitivity, induced only by strenuous exercise. Identification of this new syndrome illustrates classical epidemiologic analysis, improves medical advice for the allergic and athletically inclined, and raises new questions in the areas of allergy and immunology.


Complement activation following intravenous contrast material administration. Untoward reactions to iodinated contrast material (ICM) continue to be a significant medical problem. Complement components were assayed in patients having reactions to evaluate the possibility of complement activation in these reactions. Reactor group samples were drawn from patients during the contrast reactions and then 2 weeks later to provide the equivalent of preinjection baseline samples, Control patient values were determined from blood samples drawn from patients before and 20 minutes after ICM injections without reaction. There were consistent falls in total hemolytic complement (CH50) as well as C3, C4, and C5 in both the control group and the reactor group. These falls in the complement reached statistical significance only for CH50 in the reactor group. The results indicate that complement activation occurs after ICM injection. This activation can occur independent of clinical reactions; however, falls in total hemolytic complement and low hemolytic complement titers prior to ICM infusion may be related to the presence of anaphylactoid symptoms.


Human sensitization to Ganoderma antigen. Continuous air sampling with a Hirst volumetric spore trap over 3 yr has identified basidiospores of Ganoderma applanatum, a bracket fungus, as the most numerous fungal spores in two southern Ontario locations. The particle size is small and the calculated total spore mass approximates that of the spores of Cladosporium and Alternaria. Extracts of Ganoderma applanatum bracket fungus and spores in w/v, 1:10 concentration were prepared after collection of samples of the fungus from local woods. Skin prick tests with the extracts were performed in 294 consecutive children and adults attending two chest/allergy clinics. Of these patients, 182 (61.9%) reacted to 1 or more of the common inhalant allergen extracts and 24 (8.2%) reacted to Ganoderma antigen. There was no consistent relationship between reactivity to Ganoderma antigen and any of the common inhaled allergens. IgE-dependent sensitization to Ganoderma was confirmed by the radioallergosorbent test (RAST). Rabbit antisera to Ganoderma antigen preparations did not appear to cross-react with preparations of the various clinically important allergens. The findings indicate that Ganoderma antigen is commonly encountered, can induce human sensitization, and has unique antigenicity among common allergens of clinical importance.


Incidence of bronchoconstriction due to aspirin, azo dyes, non-azo dyes, and preservatives in a population of perennial asthmatics. Forty-five patients with moderately severe perennial bronchial asthma were challenged by ingestion of: acetylsalicyclic acid (ASA); 4 azo dyes (tartrazine, sunset yellow, amaranth, and ponceau); 3 non-azo dyes (erythrosine, brilliant blue, and indigotin); sodium benzoate (NaB); parahydroxybenzoic acid (OHBA); butylated hydroxyanisole (BHA); and butylated hydroxytoluene (BHT). A fall in forced expiratory volume is one second (FEV1) greater than 25% from baseline was considered positive. Seven patients who gave an unequivocal history of aspirin intolerance were not challenged with ASA; an additional 13 had positive open challenges to ASA, giving an apparent incidence of aspirin sensitivity of 20/45. The presence of nasal polyps, simusitis, or the regular use of corticosteroids, either singly or in combination, was not associated with an increased incidence of reactions to ASA. Significant bronchoconstriction to open challenges with agents other than ASA was less frequent. Positive open challenges to all substances except aspirin were followed by double-blind challenges which were positive in only 3 instances: 1 each with erythrosine, ponceau, and NaB/OHBA. Our findings confirm that ASA intolerance is relatively common but suggest on the other hand that reactions to dyes and preservatives are uncommon cause of clinically significant bronchoconstriction in moderately severe perennial asthmatics.


Cutaneous and nasal allergic responses in ragweed hay fever: lack of clinical and histopathologic correlations with late phase reactions. The present study was designed to test the hypotheses that late cutaneous and nasal responses to allergen in patients with ragweed hay fever were human correlates of cutaneous basophil hypersensitivity, and that late responses in the nose and skin reflect similar pathogenesis. Forty-seven patients with ragweed hay fever were studied during a ragweed season for peripheral basophilia and clinical patterns reflecting late responses. Provocative nasal challenge, skin testing, and biopsy were carried out subsequently in 21 of the same patients during the winter months. Conclusions were as follows: (1) no histologic features distinguish positive from negative late skin reactions at 24 hr in patients with immediate wheal-and-flare responses; (2) cutaneous basophil hypersensitivity, i.e., tissue basophilia, is not a distinguishing feature of late skin responses in ragweed pollenosis; (3) seasonal peripheral basophilia was not found; (4) late responses in the nose were difficult to document objectively and did not correlate with late skin reactions; and (5) lymphocyte responses to antigen failed to correlate with late responses in either the nose or the skin.


Effects of dialyzable leukocyte extracts (DLEs) with transfer factor activity on leukocyte migration in vitro. III. Characterization of the antigen-independent migration inhibition factor in DLEs as a neutrophil immobilizing factor. In earlier evaluations of the agarose LMI assay as an in vitro test for studying the nature and mechanism of action of TF, we reported the existence of a component in human DLEs which caused noncytotoxic inhibition of the random migration of human PMNs. The LMI was not dependent on the stimulation of viable mononuclear leukocytes by antigen or mitogen to effect the release of mediators of cellular immunity such as LIF; rather, the LMI was promoted by the direct action of a preexisting component in DLEs on PMNs. We now present evidence that this "antigen-independent" LMI activity in DLE'S is similar to a NIF shown previously by Goetzl and co-workers to be present in acid extracts of leukocytes and to be released by phagocytosing PMNs. The comparison is drawn from several parameters: (1) cellular origin, (2) molecular weight, (3) target cell, (4) susceptibility to inactivation by heating or by incubation with pronase, trypsin, or chymotrypsin, and (5) ability to cause noncytotoxic inhibition of random migration or chemotaxis of PMNs.


Social determinants in food selection.

Social determinants in food selection. Responses of 112 low-income homemakers to questions about the food use frequency, availability, and social determinants (convenience, price, and prestige) of fifty-two food items are reported. Results indicate that foods have clear meaning profiles that can be measured on attitudinal scales. Bivariate analysis was used to illustrate the interrelationships between food-use frequency and social determinants. Significant correlations were found between food use and convenience, price, prestige, and availability for fourteen of the fifty-two food items studied.


Menu planning competencies in administrative dietetic practice. I. The methodology. This research was intended to develop a methodology to analyze competencies in one area of administrative dietetic practice--menu planning. Five basic competencies, with sub-competencies, in menu planning were drawn from previous research. To produce descriptive statements for each sub-competency, taped interviews were held with twenty practitioners to ascertain knowledge, attitudes, and skills required in menu planning. The original 607 responses were reduced to ninety-two by eliminating duplication and ranking them by importance. These were then expressed in appropriate behavioral terminology and validated by an educational consultant. The competency statements and sub-competencies with the ninety-two descriptors were incorporated into a questionnaire submitted to a nationwide sample of hospital administrative and generalist dietitians for further validation.


Menu planning competencies in administrative dietetic practice. II. Practitioners' ratings of competence. A nationwide sample of administrative and generalist dietitians rated five competencies with sub-competencies and ninety-two descriptors related to menu planning. Each item was rated numerically for importance and frequency of time consideration. Data were grouped according to three levels of practitioner experience. It was concluded that an effective methodology was developed for analyzing competencies for dietetic practice.


Frequency of selection of food groups by low-income families in southwestern Mississippi. Food resources and frequency of food use of 1,000 households in Claiborne County in southwestern Mississippi were studied and related to income and educational level of the family heads. Income and educational levels of the family heads were much lower than the national average. The data on food frequency use indicated that the meal patterns of these households could not provide sufficient nutrients for individual family members. Income and educational levels of the family heads correlated highly with food frequency scores. The households with higher incomes and more education had better food consumption patterns than those with lower incomes and less education. However, income had more effect on low nutrient intake than education.


Nutrient estimates from computerized questionnaires vs. 24-hr. recall interviews. A computerized nutritional assessment service used by dentists was investigated. Daily nutrient intake of forty-six adult subjects was estimated from replies to a computerized food frequency questionnaire and the results compared with the average nutrient intakes of the same individuals obtained during five 24-hr. dietary recall interviews. Although the two methods produced essentially the same mean for carbohydrate intake and a minimal difference in the mean caloric values, mean estimates of the other nutrients were 6 to 88 per cent greater with the computerized data than via the dietary recalls. The dietary assessment service included unwarranted recommendations for vitamin and mineral supplementation.


Attitudes and food habits--a review.

Attitudes and food habits--a review. The inadequacy of a definition of attitudinal terms has complicated the interpretation of many food habit studies. This review has focused on the multidimensional character of the word "attitude" as an aid to gaining insight into food habit development and change. The five definitional categories presented (attitudes as preferences, as overt food behavior, as willingness or ability to change, as agreement among family members, and as complexity of meanings) do not preclude other definitions. Knowledge of attitude studies can foster understanding of the link between nutritional knowledge and actual behavior, as well as help practitioners to be more effective in formulating objectives and developing techniques for nutrition education. The various dimensions and meanings of attitudes indicate the need for continued research to clarify the effect of well defined attitude variables on food habits.


Influence of oral contraceptives on ascorbic acid and triglyceride status. The overall purpose of this study was to determine the influence of oral contraceptive agents on ascorbic acid and triglyceride status of young women by measurement of plasma and whole blood ascorbate and serum triglycerides on three days during one oral contraceptive or menstrual cycle. The subjects' food intake records were evaluated to determine the relationship between dietary intake and ascorbic acid blood levels. Results indicate that, with adequate intake of ascorbic acid, there in no threat to ascorbic acid status as a result of using oral contraceptives for periods of six months to seven years. Although bordering on being significant, the serum triglycerides did not appear to be alarmingly elevated.


Zinc and copper in self-selected diets.

Zinc and copper in self-selected diets. Levels of zinc and copper in the self-selected diets of twenty-two men and women were determined by atomic absorption spectrophotometry. Sixty-eight per cent of the subjects consumed less than two-thirds of the recommended allowance for zinc (15 mg). Eighty-one per cent consumed less than two-thirds of the suggested level of copper (2.0 mg). Average daily intake of protein exceeded the recommended allowance. However, the mean zinc:calorie ratio for those subjects was only 4.19 mg. per 1,000 kcal. Using the recommended allowances for zinc and for energy, the zinc:calorie ratio would be 6.5 mg. per 1,000 kcal. For copper, the suggested nutrient density is 0.85 mg. per 1,000 kcal vs. the 0.50 mg. per 1,000 kcal observed in this study. These metallo:calorie ratios indicate that the nutrient density of these two trace elements in the diet is low and that diets supplying adequate amounts of energy and protein do not guarantee adequate levels of zinc and copper.


Prevalence of the "Chinese restaurant syndrome". A questionnaire survey attempted to define the prevalence of symptoms characteristic of the "Chinese restaurant syndrome" (CRS) in the general adult population. Forty-three per cent of 3,222 respondents associated unpleasant symptoms with specific foods and eating environments; however, only 1 to 2 per cent reported symptoms characteristic of the CRS, and only 0.19 per cent associated these characteristic symptoms with consumption of Chinese food. Most respondents who were "aware" of the syndrome and most of those who believed they had experienced it reported non-specific symptoms. If the word "syndrome" is to be used to describe symptoms attributed to specific food ingredients, the limits of the "syndrome" must be specified.


Composition of traditional Hopi foods.

Composition of traditional Hopi foods. Foods produced by the modern Hopi Indians and prepared in traditional ways are generally good sources of essential minerals, especially if plant ash or native crude salt are added in preparation. The predominant whole grain, mature maize-corn products are relatively high in phytate, which may interfere with absorption of some of the minerals present. The use of culinary ash may have played a critical role in maintenance of nutritional health in the presence of interfering substances in the diet, especially if animal foods were in limited supply.


Carbohydrate loading--a review.

Carbohydrate loading--a review. The purpose of carbohydrate loading is to supersaturate with glycogen the muscles to be used in competition. The competition should be longer than 30 to 60 min. to fully utilize the glycogen stores. An exhausting exercise is first performed to deplete the glycogen stores, and a high-fat, high-protein diet is followed for three days to keep the glycogen stores low. After depletion of the muscles, a high-carbohydrate diet is followed for two to three days to restore and supersaturate the muscles with glycogen. The most important point to impress on the athlete is the nutritional adequacy of the entire diet. Though the technique of carbohydrate loading is a dietary manipulation emphasizing the intake of carbohydrate, the diet can be adequate with sound dietary planning.


Acohol use among older persons: findings from a Western New York State general population survey. This report on the drinking patterns of older persons is based upon a cross-sectional probability sample of 1,041 adults aged 18 years or older living in Erie and Niagara Counties in Western New York State. The rates of drinking, heavy drinking, and alcohol-related problems were considerably lower among persons aged 60 or older than among those aged 50--59 (approaching old age), or among those aged 18--49 (the younger comparison group). Although heavy drinking was almost nonexistent among the elderly women, sizable proportions of the older men (e.g., about a quarter of the men in the 60--69 age group) were heavy drinkers. Previous reports concluding that the stresses of aging, such as widowhood and retirement, are associated with increased problem drinking were not confirmed in this study. More definitive, longitudinal research is needed to develop a better understanding of the relationships between drinking patterns and the aging process.


Survival in the elderly with acute leukemia. The natural history of acute leukemia in the elderly is not well understood. There is disagreement about the value of treatment other than by supportive measures. In this study, the survival predictive power of 9 variables was analyzed in 103 patients aged 70 or older for whom a bone-marrow cytologic diagnosis of acute leukemia had been made at a referral hospital during the 30-year period, 1947--1976. When given, treatment was uniformly conservative. Blood leukocyte and platelet counts and hematocrit values at the time of diagnosis, sex, treatment, and the calendar year of diagnosis were significant (P less than 0.01) predictors of survival duration. Initial hematologic measurements, presumably reflecting the degree of leukemic cell infiltration of the bone marrow, were the strongest predictors of survival, whereas leukemic cell type had no predictive power. Survival increased significantly (P less than 0.01) during the 30-year period of the study, possibly more attributable to improved supportive care than to specific antileukemic therapy. Apparently survival is at least partly determined at the time of diagnosis, and conservative therapy often may be beneficial rather than detrimental.


The type A (coronary-prone) behavior pattern, aging, and mortality. Young and middle-aged persons displaying the Type A (coronary-prone) behavior pattern have been shown repeatedly to be at substantially increased risk of coronary heart disease. A review of recent empirical research regarding adaptability and survival among the elderly suggests the hypothesis that the clinical significance of Type A behavior may shift radically in old age. Thus, compared to Type B (not coronary-prone) subjects. Type As may be at lesser rather than greater risk of impairment and death.


Modern ideas about organization of services for elderly patients in hospitals and nursing homes in the Netherlands. The system of nursing homes in the Netherlands, and the advantages of the new department for geriatric assessment in the Municipal Hospital in Amsterdam are discussed. Included are admission and discharge data on 694 patients in the Geriatric Department in 1977, and also a list of the more common diagnoses, classified according to body system and disease.


The misplaced-objects task: a brief test for memory dysfunction in the aged. A brief, simple test for memory dysfunction in the aged is presented. Materials for the test consist of: 1) a board on which the cross-section of a seven-room house is imprinted, and 2) representations of 10 objects frequently misplaced in the home (e.g., keys). The task of the subject is to place the objects in rooms on the board and, after an interval of 5--30 minutes, to recall the location of each object. The measure is appropriate for a broad range of aged subjects, since performance requirements are minimal and readily apparent. The task has obvious face validity, which is essential for maintaining appropriate motivation in many aged subjects. Discriminate validity was demonstrated in a study in which significantly fewer objects were recalled by 60 aged persons with memory impairment (-x = 6.0) than by 44 unimpaired aged persons (-x = 8.9) or 63 young normal persons (-x = 9.9). Satisfactory test-retest reliability was demonstrated by a correlation of .84 between performances on two consecutive days in a subgroup of 20 impaired patients. Subjects appeared to enjoy the game-like structure of the test and clearly were not threatened by the test procedures. Suggestions are offered for further development of the measure.


Health-seeking behavior and social networks of the aged living in single-room occupancy hotels. The elderly who reside in single-room occupancy (SRO) hotels often have been depicted as "isolates," lacking the interest or ability to engage primary or secondary support systems. This characterization has not enhanced understanding of how the SRO aged are able to survive in the community. With the use of network analysis techniques, this study demonstrates the inaccuracy of the assertion that these old persons lack significant social support. The data pointed to differences in network size, complexity, intensity, connectedness, and directionality in relation to varying degrees of physical and psychiatric health.


An alternative model in geriatric care.

An alternative model in geriatric care. An analysis is presented of one long-term care facility's attempt to develop a comprehensive geriatric program including care of the patient in a hospital, a skilled nursing home, a day hospital, or a clinic for care of the ambulatory aged. The goal was to raise issues for debate and discussion, especially in terms of the evolving role of medical directors in long-term care. The need for group professionalism in cooperation with the Board of citizens in community geriatric practice is emphasized.


Skin oxygen tension as a function of imposed skin pressure: implication for decubitus ulcer formation. Pressure is a crucial factor in the formation of decubitus ulcers. To elucidate the effect of imposed skin pressure, a new cutaneous oxygen sensor was used to measure the skin oxygen tension under increasing pressure upon skin tissue at "hard sites" (bony prominences) and at "soft sites" (muscle-padded areas). At hard sites the skin oxygen tension fell rapidly under increasing pressure (y = 90.9--0.39. X; r = 0.98) from an initial value of 86.4 +/- 10.6 to 20.2 +/- 12.1 mm Hg under an imposed skin pressure load of 175 gm/cm 2. At soft sites a pressure load of 175 gm/cm 2 decreased the skin oxygen tension only from 82.9 +/- 5.8 to 71.4 +/- 10.0 mm Hg. These results may explain why hard sites (bony prominences) are preferential areas for decubitus ulcer formation.


Cancer in the aged: an autopsy study of 940 cancer patients. In an autopsy study of 940 elderly cancer patients, 1,030 cancers were identified. The prevalence rate for overall cancer declined after age 85 in men and after age 75 in women. The chief sites of major cancers were the stomach, lung, esophagus, liver, and pancreas, in that order. Incidental cancers (chiefly of the prostate, thyroid, and colon) were found more often in patients over 80 years old. For multiple primary cancers, the prevalence rate was relatively constant until the age of 70, when it rose to a peak in the 80--84 age group before declining to the original level.


A structural model of functional capacity in the aged. The core problem in geriatrics is the continuous loss of adaptive capacity by bones, joints, brain, heart and lungs. Thus many old persons cannot take care of themselves any longer in matters of food, safety, body temperature and hygiene, the basic functions for human independence. Geriatricians agree about the basic functions that an elderly person should be able to perform. Only with such criteria can goals be defined more accurately and comparisons made of therapeutic efforts, leading to a higher level of efficiency in therapy, prognosis and care. A theoretical framework is proposed which may serve as a model for assessment of functional capacity in the aged patient. The model (hierarchic structure) has four components: household activities, mobility, activities of daily living, and function of the autonomic nervous system. No component covers more than five items. There is great need for studies on an international level so that assessments and treatments of elderly patients can be compared.


Topical application of povidone-iodine in the management of decubitus and stasis ulcers. The efficacy of topical applications of povidone-iodine (Betadine solution and ointment) for the control of infection associated with decubitus and stasis ulcers was evaluated in 18 male outpatients (age range, 33--68 years). Dressings were changed twice daily over a period of 42 days. Statistically significant improvement was noted in the following signs and symptoms: edema, pain, erythema, ulcer size, and ulcer depth. All patients experienced some degree of symptomatic relief and clinical improvement within two weeks after starting therapy. At the end of the study, 67 percent of the ulcers were clinically cured and 33 percent showed improvement. Acceptance by the patients was excellent, with no reported side effects or sensitization reactions. Povidone-iodine used in a daily regimen of ulcer care can reduce the level of infection and promote healing.


The corneal reflex in elderly patients.

The corneal reflex in elderly patients. The corneal reflex was absent in 23.5 percent of 115 elderly patients, particularly in those with cerebrovascular disease. However, it was also absent in many of the patients without neurologic disease. Therefore, physicians should not attach too much importance to lack of the corneal reflex in elderly persons.


Palatal sensitivity to touch: correlation with age. In 100 persons whose ages ranged from 20 to 89 years, palatal sensitivity to touch (by air current) was measured. Sensitivity diminished progressively with age.


Toward a comprehensive geriatric educational experience. A description of the goals and the accomplishments of a program in geriatric education and training is presented. Emphasis is laid on the potential role of departments of family and community medicine, even though vast resources may not be available. Such a program is of great importance, but it must be viewed as only the first step in the development of a comprehensive training model for either a school of medicine or a residency program in primary care. Upon graduation in Family Medicine, residents should not only be aware of the specific health needs of the elderly, but also be clinically competent to meet those needs.


Rehabilitation in the extended care facility. Rehabilitation can be readily carried out in an extended care facility. A technique is presented for accomplishing this objective by organizing existing personnel within the facility into a rehabilitation team which is goal-oriented. The Barthel Index serves as an objective measurement of the patient's progress. By use of this approach, significant numbers of patients with stroke, orthopedic disorders and other neuromusculoskeletal impairments can be returned to their previous living situations in a relatively short period, and at minimal cost.


Perception of poor health in the healthy older adult. A survey of 977 community subjects aged 65 or older identified 719 of them as not impaired in physical health. Of these, 104 (14 percent) perceived their physical status to be poor, whereas 615 (86 percent) accurately perceived it to be unimpaired. There were no significant differences between the two groups with regard to age, sex, race, social class, living arrangements and number of drugs used. The physically healthy elderly who perceived their physical status to be poor were more depressed, more hypochondriacal, and more dissatisfied with life. They tended to complain of multiple symptoms; activities of daily living were decreased, and they were more likely to visit their doctor frequently during the year (analysis controlled for physical health in each case). Mental health was slightly but significantly more impaired in these subjects; nevertheless, they were more likely to seek the help of a trained counselor. The findings suggest that a self-perception of poor health among the physically healthy elderly may represent a generalized request for attention from trained personnel in the social environment.


Mechanism of gas transport through contact lenses. The transport of oxygen and carbon dioxide through contact lenses is important for maintaining normal corneal physiology. Gas permeation through a lens depends on the solubility and the diffusivity of the lens for that gas. Gases pass through spaces between the flexible polymer segments in plastics and rubbers. Gases diffuse through the water of hydration in hydrogel lenses, and gas permeation increases exponentially with hydration. The driving force behind gas permeation is the difference in partial pressure of the gas across the lens. The amount of gas passing through a given lens, in unit time, doubles by halving the lens thickness. Carbon dioxide permeation through any lens is several times greater than is oxygen permeation through that lens. Thus, it suffices to know the oxygen permeability in order to predict the physiological performance of a lens. The oxygen permeability coefficients of representative hard, hydrogel, and silicone rubber lenses are tabulated for easy reference.


Clinical evaluation of FDA approved toric hydrophilic soft contact lenses (Part I). Three toric hydrogel contact lenses have been approved by the FDA, Durasoft TT, Hydromarc, and Hydrocurve II. These all incorporate prism ballast as the major axis stabilizing feature, but differ in diameter and placement of the cylinder. Correct cylinder axis positioning and maintenance of meridional stability are essential to fitting success.


Basic considerations in fitting hydrogel lenses. Recommended guidelines for evaluating the performance of hydrogel lenses are developed based upon the physiological reponse of the cornea.


Residual astigmatism and visual acuity with hydrogel contact lenses: a comparative study. Residual astigmatism, visual acuity, and transferred corneal toricity were measured in a double masked fashion on 24 randomly selected patients fitted with Buasch and Lomb Soflens contact lenses, the Hydrocurve II contact lenses, and the AOSoftTM hydrophillic contact lenses. None of the subjects had any contraindications to hydrogel lens wear. All three lens types produced a small but similar reduction in refractive astigmatism. However, because of residual astigmatism, fewer eyes achieved 20/20 or better visual acuity with each of the hydrogel lens types than with the best spectacle correction. Corneal toricity was found to be transferred to the front surface of all three lens types. Our findings indicate that generally these three types of hydrogel lenses result in similar residual astigmatism, visual acuity and transferred corneal toricity. However, individual differences may occur. Therefore, the choice of lens type should be based on the individual patient's response to various lens types.


Lacrimal lens effects in Bausch and Lomb Soflens contact lens wear. The correlation between lacrimal lens power changes and changing posterior apical radii was examined for a sample of 20 eyes wearing Bausch and Lomb Soflens contact lenses. Power changes caused by in situ lens flexure were calculated for principal meridians and both calculated in situ contact lens powers and over-refraction corrections were subtracted from the patient's spectacle corrections to determine lacrimal lens powers in each meridian. A significant correlation (p less than 0.01) was found in the flatter meridians for B, F, J, and N series lenses. To support the validity of the study a correlation was done between K's calculated from refractions done over the Bausch and Lomb Soflens contact lenses and both the K's calculated from refractions done over PMMA diagnostic lenses and the K's as measured with the keratometer. There was a significant (p less than 0.01) correlation in both series, indicating that calculated K's can validate the presence and power of a lacrimal lens in soft (hydrogel) contact lens wear as well as in conventional contact lens wear. (A correlation between the K's as measured with the keratometer and the K's calculated from refractions done over PMMA diagnostic lenses were also significant (p less than 0.01).


Rigid lenses: an overview.

Rigid lenses: an overview. New gas permeable rigid contact lens materials, by allowing direct transmission of oxygen, provide significant advantages over PMMA. Edema resulting from oxygen deprivation with PMMA lenses is eliminated and comfort is increased. Three types of gas permeable materials are described: CAB, silicone, and a combination of CAB and silicone.


Oxygen-transmitting hard contact lenses. Nine PMMA contact lens wearers with persistent corneal edema were refitted with CAB contact lenses. All of the patients showed less corneal swelling with CAB lenses than with PMMA lenses.


Effects of rigid lens flexure on vision. Rigid lenses may flex on the eye, particularly when the center thickness is less than 0.12 mm. Rigid oxygen permeable materials are less rigid than PMMA and can be expected to flex more easily.


Giant papillary conjunctivitis: an overview. Giant papillary conjunctivitis is a condition of the upper palpebral conjunctiva which has been associated with hard and soft contact lens wear. The condition is classified into four stages. The causes and course of therapy are discussed.


New design concepts for permeable rigid contact lenses. Gas permeable rigid lens materials offer the opportunity to reevaluate contact lens design. This paper presents the rationale and procedures followed in the development of a design concept for the Polycon lens material.


Tear pH: resistance to change.

Tear pH: resistance to change. While considerable study and clinical speculation has been given to tear pH, almost no attention has been paid to the buffering capacity of this fluid. Responses of tears to an alkaline challenge test as a measure of their buffering capacity are described here for a series of patients. The magnitudes of those resulting shifts, and the probable mechanisms influencing them are commented upon.


Corneal staining relating to contact lens wear. This paper presents an overview of the common types of corneal staining that are encountered when wearing contact lenses. Included are descriptions of corneal staining occurring with rigid lenses (polymethylmethacrylate and cellulose acetate buterate), various types of hydrophilic lenses and silicone lenses. Specific patterns of sodium fluorescein staining are depicted and related to the various types of contact lenses.


Corneal staining subsequent to sequential fluorescein instillations. Liquid fluorescein (2%) was instilled into the inferior cul-de-sac and the precorneal tear film and the cornea were observed utilizing a specialized examination routine. Following the initial fluorescein instillation and observations, sequential fluorescein instillations were performed at five-minute intervals six additional times. The incidence of corneal staining (200 corneas) following the initial instillation of fluorescein was 19%. An additional 23% exhibited staining as a result of the sequential staining process. Five types of staining were classified; the most common forms were those associated with the lid margins. Severe degrees of staining appear to be clinically significant, as suggested by a correlation between severe degrees of staining and contact lens intolerance.


An embedded contact lens in the upper lid masquerading as a mass. A patient who "lost" his right contact lens three years prior to our examination was found to have a mass in the right upper lid which contained the hard contact lens. A careful search of the superior fornix and the upper lid, with double eversion of the lids, is indicated in instances where a patient presents reporting loss of a lens he was wearing or inserting.


Effects of the antioxidant butylated hydroxytoluene (BHT) on mortality in BALB/c mice. Butylated hydroxytoluene (BHT) was given in the feed to determine its effect on life span in genetically well-defined, barrier-derived BALB/c mice. Both sexes received 0.75% BHT for three different treatment periods: (A) 8 to 11 weeks of age; (B) for life, beginning at 11 weeks; (C) for life, beginning at 8 weeks of age. The control group (D) was untreated. All BHT treatment groups had mean survival times which exceeded that of controls. The order of survival was B greater than C greater than A greater than D (Males: 890, 832, 726, 684 days; Females: 875, 798, 759, 701 days). Most of the increases in mean survival time were related to a reduction in early deaths (350--600 days) in BHT-treated mice. The reason for the life-lengthening effect on BHT was not identified, but it may relate to alterations in specific disease incidences.


Morphologic lesions in aging Syrian hamsters. The oldest members of most short- and long-lived mammalian species develop many similar morphologic changes. This suggests that the combined occurrence of a variety of age-associated lesions in a group can be used as an indicator of its advanced biological age. Males and females from our colony of aging Syrian hamsters were previously shown to have an equally high incidence of atrial thrombosis and myocardial degeneration, despite the females' much shorter life-span. Other age-associated lesions were then examined histopathologically to determine whether females age faster than males. Hepatic, renal, and splenic amyloidosis were more severe in females than in males and became so at an earlier age. Degenerative lesions were also found in adrenals, thyroids, and brains of both sexes. Atrophy was especially severe in the thymus. The incidence of malignant neoplasms, most of which were of lymphoreticular origin, was similar in both sexes. Female hamsters may age faster than males if biological age can be assessed by these morhologic criteria.


Age and aerobic power in women: a longitudinal study. Thirty-six women from an original cross-sectional population of 81 were retested after an average time span of 6.1 years to determine the longitudinal effects of aging on aerobic power. Women in their 20s maintained a constant level of VO2max. All other age groups showed a decrease in aerobic power (l . min-1 and ml . kg-1 . min-1) similar to that observed in the cross-sectional study. Although the decline in VO2max was similar for both groups, active women increased walk time on the treadmill, while sedentary women decreased in endurance time. Maximal heart rate also decreased with age but the rate of decrease was not constant across time. HRmax remained relatively steady during the middle years and then declined at a faster rate in the 50- and 60-year-old age groups. The results of the present study suggest that cross-sectional studies can provide valid information about the effect of age on response to maximal exercise provided that close attention is paid to the limitations underlying regression therapy. The data also indicate a need to consider alternatives to linear regression analysis of aging effects, since the rate of change of some variables is not constant across age.


Serum ferritin in an elderly population. Serum ferritin was assayed by an immunoradiometric method in 82 people above 60 years of age. For comparison purposes, the same assay was performed in 71 younger normal adults. The serum ferritin distribution in the elderly had a larger variance than in the younger adults and in addition, there was a clear shift to higher values in the elderly. The latter was more notable in females than in males but there was still a statistically lower mean in elderly females than in elderly males. Ten out of 55 elderly subjects with evidence of iron deficiency (response to oral iron therapy) had a normal or high serum ferritin which suggests that variables unrelated to iron status may operate in determining serum ferritin levels in the elderly. The shift to higher values appears to occur upon reaching grossly 70 years of age. Whether the shift is a physiologically normal event is at present an open question.


Cognitive speed and subsequent intellectual development: a longitudinal investigation. The hypothesis that a measure of intellectual speed assessed at one point in time would predict intellectual achievement at a later point in time was evaluated with a time-lagged cross-correlational analysis, an application of causal modeling techniques. Longitudinal data for 32 males and females, tested in 1944 (mean age 19.5 years) and in 1972 (mean age 46.7 years), supported the hypothesized relationships with an associated p less than .01. The Relations Factor of the Army Alpha Examination--consisting of scores from a highly speeded simple analogies test and a short-term memory test--administered at age 20 was highly predictive of both verbal and numerical ability in middle age. The results highlight the cognitive intellectual aspect of the speed of behavior. In addition, these findings supplement Hunt's studies of the relationships between speed of cognitive processing and psychometric abilities in young adults, and emphasize the importance of cognitive speed for subsequent intellectual development. Implications for the intellectual speed hypothesis of Birren and the utilization of time-lag designs in longitudinal research are discussed.


Age differences in adults' free recall, cued recall, and recognition. Adults in their twenties and sixties were tested for free recall, cued recall, and recognition of words that they had studied in an intentional memory task or generated associations to in an incidental orienting task. Significant age-related declines in performances on intentional items were observed regardless of type of memory test. Significant age-related declines in performance on incidental items were also observed on free and cued recall tests; however, age differences on incidental items were attenuated on cued recall tests, and they were eliminated on recognition tests. In addition, while younger subjects profited more from retrieval cues that were associations they had themselves generated than from retrieval cues that were normed common associations, older subjects made comparable use of these two cue types.


Age differences in resolving power and decision strategies in a weight discrimination task. Current theories of aging suggest that at least part of the decline in performance on psychophysical tasks, commonly observed in older subjects, may be the result of their becoming more cautious about sensory decisions. This conclusion has been supported by experiments in which the criterion index, beta, from signal detection theory is shown to be higher (indicating a strict criterion) for older subjects. A weight-discrimination experiments is reported which failed to replicate the earlier results; older subjects' criteria were the same or more lax than those of younger controls on that task. The possibility that extreme caution on the part of older people may be specific to certain classes of task is discussed.


Effects of varying irrelevant information on adult age differences in problem solving. Sixty young (M = 20.6), middle-aged (M = 52.4), and elderly (M = 72.6) men and women solved problems which required them to match one of two stimulus arrays to a standard. On each problem one dimension (color, form, number, or position) was relevant to correct matching, and three dimensions, which were either variable or constant, were irrelevant to solution. Age and the number of variable irrelevant dimensions were the best predictors of reaction time and error scores. Young were significantly faster than middle-aged and the middle-aged were faster than the elderly. The elderly made most errors, but the young and middle-aged were not significantly different from each other. Reaction times and errors increased as the number of variable irrelevant dimensions increased. For the elderly there was a disproportionate increase in both reaction times and errors as levels of irrelevancy increased. No reliable differences were found with regard to gender. The results were discussed in terms of an age-related decline in the ability to ignore irrelevant information.


Old men and young women: how objectively are their skills assessed? Seventy-four male and female undergraduate participants evaluated an essay describing a work of art. They read either a well or a poorly written essay attributed to one of the following authors: a 25-year-old male, a 25-year-old female, a 64-year-old male or a 64-year-old female. Age of target X sex of target X essay quality interactions emerged on four of five dependent variables. In general, essay quality affected evaluations of young male and old female authors, but had little impact on evaluations of young females and old males. The results suggest that young females and old males may be the target of subtle prejudice which causes their work to be assessed unobjectively. They also emphasize the importance of simultaneously manipulating age and sex of target persons in future research. Implications of the findings for the accurate evaluation of elderly males in employment settings are discussed.


Anomia, self-esteem, and life satisfaction: interrelationships among three scales of well-being. The extent to which anomia, self-esteem, and life satisfaction are conceptually distinct was studied by using factor analysis of the items from Srole's anomia scale, Rosenberg's scale of self-esteem, and the LSI-Z. The data came from interviews held with 1,332 older men living in nonmetropolitan areas of Iowa. The analysis demonstrated that, while the concepts of anomia and self-esteem are distinct, the domain of life satisfaction overlaps those of anomia and self-esteem. The representation of the five components of life satisfaction by the items of the LSI-Z is questioned, and caution in using the six-items version of Rosenberg's measure of self-esteem with older adults is suggested.


Age variations in the dimensionality of life satisfaction. Paralleling the increasing importance of life satisfaction and related concepts in gerontological research has been an increase in the number and kind of operational measures of these concepts. In this context the present research suggests not only that life satisfaction is a multidimensional rather than a unidimensional construct but, of particular gerontological importance, the pattern of multidimensionality varies across age groups. The analysis is based upon a national sample of the adult population of the United States in which 12 different domains of life satisfaction were subjectively assessed by each respondent. Separate factor analyses were computed for each of seven age groups. The results clearly indicate that for every age group life satisfaction is a multidimensional construct. More importantly, the dimensionally differs substantially, across the age groups -- both in the number of factors derived and the domain-specific content of those factors.


The voluntary associations of the aged.

The voluntary associations of the aged. This paper examines the voluntary associations of the aged. A substantial majority of respondents were affiliated and more than one-half had multiple affiliations. Rate of affiliation remained high even for individuals over 80. Contrary to expectation, women were as likely as men to be affiliated and to have multiple memberships, and were significantly more active in voluntary groups than men. Those belonging to multiple associations were more active in each association than individuals with only one or two affiliations. Overlapping memberships were common. Reasons for active involvement are provided. The bulk of the memberships were of three types which, in function, are integrative at the personality level.


The antitrust implications of relative value studies in medicine. Relative value studies are lists of "relative values" of different professional services. Antitrust enforcement agencies recently have filed several complaints against the promulgation of relative value studies by professional organizations of physicians, alleging that these lists have been used to fix and increase physician fees. This article examines the status of professionally sponsored relative values studies under antitrust law and suggests several reasons why they should be held unlawful. In particular, such relative value studies threaten to eliminate desirable competition among private third-party payers in the development of effective cost-containment strategies as well as among physicians in the setting of fees. Moreover, the alleged benefits of professionally sponsored relative value studies could be achieved by alternative means that do not similarly restrict competition in the provision of medical services.


The development of the sinusoids of fetal rat liver: localization of endogenous peroxidase in fetal Kupffer cells. Endogenous peroxidase is the cytochemical marker used to identify Kupffer cells in the adult liver. In this study, we show by ultrastructural cytochemistry that Kupffer cells of the fetal rat liver are endogenous peroxidase positive. The reaction product is localized in the endoplasmic reticulum including the perinuclear cisternae and in a few lysosome-like dense bodies. Serial sections of Golgi regions suggest that GERL and not the Golgi stacks, is peroxidase positive. As in the adult liver, peroxidase is not localized in endothelial cells. Kupffer cells do not appear to transform from endothelial or extravascular developing monocytic cells and are present prior to bone marrow formation. The relevance of these observations with respect to the possible origin of the Kupffer cell is discussed.