The quantitative swab culture and smear: A quick, simple method for determining the number of viable aerobic bacteria on open wounds. The quantitative swab culture is a reliable method for quantifying the number of viable bacteria colonizing open wounds. For open wounds in burn patients, the swab bacterial count is linearly related to biopsy quantification of viable bacteria in the underlying tissue. This technique is simple and requires no surgical manipulation of the wound. For a wound of uniform appearance, the standard deviation of a single swab culture from the mean log bacterial count for a series of cultures from widely spaced areas on the wound is +/- 0.85 logs; 95% confidence limits are +/- 1.7 logs from the mean. A Gram-stained smear from a wound swab requires less than 10 minutes to prepare. Visualization of bacteria on the smear indicates that 106 or more bacteria per swab are present. The value of the smear and swab techniques for predicting safe wound closure may be inferred from the published reports of others and the direct relationship between the swab and biopsy counts of viable bacteria for open wounds.


Entrapment of the tibialis anterior tendon as a cause of nonunion in fractures of the distal tibia. Three cases of nonunion of a fracture of the distal third of the tibia are reported. The cause of the nonunion in all three cases was the entrapment of the tibialis anterior tendon between the bone ends. This has not been previously reported as a specific cause of nonunion of the distal shaft of the tibia. This paper draws attention to this occurrence as a possible cause of nonunion in this area especially if the fracture is of the oblique type with an anterior spike at the distal end of the proximal fracture, with the line of fracture running parallel to the line of the tendon of the tibialis anterior. We suggest that fractures of this type should be treated early by internal fixation in view of the complications that can occur, such as nonunion, atrophy, or even complete rupture of the tendon of tibialis anterior, leading to a residual equinus deformity.


The strength and failure characteristics of rat medial collateral ligaments. Traumatic loading of the knee joint in man occurs at strain rates ranging from 0.5-1,500 m/m/sec. Experiments on the medial collateral ligament of rats demonstrate that the mode of failure is a function of the strain rate. Avulsions occurred more often at low rates; ligament tearing occurred more often at high rates.


Traumatic dislocation of hip joint with fracture of shaft of femur on the same side. Four cases of traumatic dislocation of the hip joint with fracture of the shaft of femur on the same side, and one case of bilateral hip joint dislocation with a fracture of shaft of femur on one side are reported. Pitfalls in diagnosis and hazards of delayed treatment are emphasized. Methods of treatment are outlined. Complications such as avascular necrosis of the head of the femur and sciatic nerve palsy are discussed. Avascular necrosis of the head of the femur is not inevitable even after reduction of the joint has been delayed for several days.


Use of 133xenon in early diagnosis of inhalation injury. 133Xenon lung scanning post-thermal injury was used to detect inhalation injury in 86 patients admitted to the United States Army Institute of Surgical Research during 1974. Inhalation injury was indicated by the 37 (43%) positive scans. Based on all available clinicopathologic evidence, 11 (13%) of the scans were erroneous with seven (8%) falsely positive and four (5%) falsely negative. Eighty-six per cent of the scans were "appropriate." Addition of bronchoscopy and/or pulmonary function testing appeared to improve diagnostic accuracy. By using any pair of tests, falsely negative diagnoses were virtually eliminated. Inhalation injury, as expected, had an adverse effect on survival rates. The group of patients whose expected mortality lay between 40 and 59% were most notably affected. One hundred per cent (five of five) of those with inhalation injury died; only one of eight without inhalation injury died.


Lateral collateral ligament ankle injuries in young athletic individuals. 1. Eleven cases of surgical repair of acute tears of the capsule, anterior talo-fibular, and calcaneo-fibular ligaments of the ankle in young athletic individuals are reported. 2. Diagnosis was made by clinical examination and stress X-rays and confirmed by arthrography in selected cases. All of the ligament ruptures were visualized at surgery. 3. Post-surgery, all of the patients had a stable ankle and were able to return to vigorous athletic endeavors without disability. 4. This study lends support to the impression that surgical repair of severe lateral collateral ankle ligament injuries in young athletic individuals leads to a functionally and mechanically stable ankle able to withstand severe stress without disability.


Jailhouse burns.

Jailhouse burns. Twelve thermally injured patients were admitted to the burn ward of the University of Cincinnati Medical Center from penal institutions in the past six years. Six case histories were reviewed. The mortality rate was 66% and there were some with pulmonary complications. The problem of fires and burns in locked facilities is reviewed and some methods of prevention discussed.


Delayed appearance of traumatic ventral hernia: a case report. An unusual traumatic ventral hernia is reported, observed 6 months after its presumed cause. The delay in diagnosis, the benign appearance of the abdomen in the supine position, and the necessity for marlex mesh prosthetic repair in a large traumatic defect are all unusual features. Following repair the patient has remained asymptomatic.


Triceps brachii rupture: case report.

Triceps brachii rupture: case report. A 44-year-old man has been seen by the present authors, apparently the third reported case of triceps brachii rupture. He had had bilateral nephrectomies one year earlier and since then has been medicated with Dilantin for grand mal seizures which followed hypovolemia during dialysis. A grand mal seizure immediately preceded the patient's right triceps brachii rupture and other multiple orthopaedic injuries. Following repair of the tendon defect the patient regained an active range of motion. The possible relationship of tendon rupture and avulsion to primary and secondary hyperparathyroidism is discussed.


Recovery of the accumulation ability of thiomethyl-beta-galactoside in Escherichia coli after bacteriophage T4 infection. Effects of UV-irridiated and unirradiated T4 phage infection on the beta-galactoside accumulation ability in Eschericia coli have been examined by the use of 14C-labeled thiomethyl-beta-galactoside (TMG). Under conditions where a synchronous adsorption of phage takes place, the cellular ability for TMG accumulation is found to be largely inhibited immediately after phage adsorption, but it recovers with time to a new level, which is dependent on the multiplicity of infection. When cells are infected with UV-irradiated T4 at the same multiplicity as that of unirradiated phage, the cellular accumulation ability is more severely inhibited and there is no recovery from the inhibition. The recovery process in T4-infected cells is mostly sensitive to puromycin. These results suggest that the initial inhibition of the TMG accumulation ability is probably caused by the adsorption of phage coats, and the subsequent restoration occurs through the action of a phage-directed protein(s). In the recovery process, no new transport system appears to be involved. The restored ability of TMG accumulation is resistant to the action of superinfecting UV phage. However, different mechanisms appear to be operating in T4-infected cells for the establishment of resistance to ghosts and for the recovery from the phage coat-induced inhibition.


Sequence of methylated nucleotides at the 5'-terminus of adenovirus-specific RNA. RNA labeled with [methyl-3H] methionine and [14C]uridine was isolated from the cytoplasm of adenovirus-infected cells and purified by poly(U)-Sepharose chromatography and hybridization to filters containing immobilized adeovirus DNA. Analysis by dimethyl sulfoxide-sucrose gradient sedimentation suggested that the major mRNA species were methylated. 7-Methylguanosine was identified at the 5'-terminus of the advenovirus-specific RNA and could be removed by periodate oxidation and beta-elimination. Structures of the type m7G(5')ppp(5')Nm containing the unusual nucleoside N6, O2'-dimethyladenosine, and smaller amounts of 2'-O-methyladenosine were isolated by DEAE-cellulose chromatography after P1 nuclease digestion of the RNA. Evidence for some 5'-terminal sequences, m7G(5')ppp(5')m6AmpNm, with additional 2'-O-methylribonucleosides was also obtained. A base-methylated nucleoside, N6-methyladenosine, is located within the RNA chain and is released as a mononucleotide by alkali hydrolysis.


Genetic analysis of adenovirus type 2 III. Temperature sensitivity of processing viral proteins. Using sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis of [35S]methionine-labeled adenovirus type 2-infected KB cell extracts, a total of 23 virus-induced polypeptides was detected. This technique was applied to the analysis of the temperature-sensitive mutant, ts 1, which has previously been shown to be defective in a late function. By means of pulse-chase experiments, ts 1 was shown to be defective in the processing of the precursor polypeptide (Pre VII) to the major core protein VII. Two other putative precursor polypeptides, Va (27K) and Vb (24K), were also not processed. Thus, the ts 1 mutation blocked the appearance of six post-translational clevage products, i. e., polypeptides VI, VII, VIII, X, XI, and XII. All of these polypeptides are virion components. Processing was temperature sensitive in a shift-up experiment, whereas it was normal in a shift-down experiment. The kinetics of the temperature-shift experiments suggested that infectious virus could be recovered if enough time is provided for processing to take place. Processing was not inhibited by cycloheximide. The analysis of purified virus particles and empty shells (TCs) revealed the presence of the precursor and putative precursor polypeptides Pre-VII, Va and Vb, instead of their cleavage products, in both types of particles. Based on these results we propose that the ts 1 gene codes for or regulates an endoprotease which is responsible for the completion of the last step in virus maturation, that is, the conversion of "young virions" into mature infectious virions by a series of maturation cleavages.


Isolation and characterization of Caulobacter crecentus bacteriophage phi Cd1. A DNA-containing bacteriophage, phiCd1, was isolated from sewage and shown to infect both stalked and swarmer cells of Caulobacter crescentus strain CB13B1a. phiCd1 is a small, icosohedral bacteriophage, 60 nm in diameter, which possesses a short, noncontractile tail, 10 to 12 nm in length. The bacteriophage particle is composed of at least eight structural proteins. phiCd1 nucleic acid exists as a linear duplex of DNA as judged by: (i) thermal denaturation (Tm), (ii) CsCl density gradient centrifugation, and (iii) chemical analysis of its base composition. The DNA is 61% guanosine plus cytosine, has a buoyant density in CsCl of 1.721 +/- 0.001 g/cm3, and denatures sharply at 78.5 C in 0.1 SSC (standard saline citrate) buffer. The S20, w value for the DNA is 34.3 +/- 0.1S as compared with T7 DNA, indicating a molecular weight of about 29 x 10(6).


Functional empty capsid precursors produced by lambda mutant defective for late lambda DNA replication. This report described lambda phage morphogenesis in a mutant system in which the normal pathways for late phage DNA (concatemer) synthesis are blocked and early (monomeric circular) DNA replication products accumulate. As shown earlier (Dawson et al., 1975) under these conditions, late proteins are synthesized and assembled into headlike structures. These structures that accumulate in the mutant are empty, suggesting the monomeric circular DNA molecules cannot be encapsulated. The present results show that crude extracts of induced lysogens of the mutant contain the complementation activities of preheads (the empty precursors to DNA-filled heads), tails, and DNA terminigenerating protein(s). Sucrose gradients of these crude extracts yield fractions containing prehead activity in relative amounts expected from the concentration of late proteins and empty structures. Furthermore, the proteins present in these fractions coelectrophorese with the known capsid proteins of preheads, and empty structures that look like preheads are observed in electron microscope examination of samples from the fractions. Based on our biological, biochemical, and electron microscope analyses, we conclude that the empty structures that accumulate in the induced lysogen of the mutant are normal preheads, which could become filled phage heads if DNA of the appropriate structure (i.e., "late DNA") were available.


Early gene expression in bacteriophage T7. I. In vivo synthesis, inactivation, and translational utilization of early mRNA's. In vivo decay rates for the individual T7 early mRNA species were determined. The physical half-lives, measured at 37 C, range from 1.1 min for gene 0.7 RNA to 4.5 min for gene 0.3 RNA. Physical half-lives, as observed after rifampin inhibition of RNA synthesis and polyacylamide electrophoresis of RNAs, are approximately 30% longer than functional half-lives, as observed by 14C-labeled amino acid uptake into individual T7 early proteins. The different RNA species are synthesized at grossly different rates, 0.3 RNA at four times the rate of 1.0 RNA, 0.7 RNA at twice the rate, and 1.1 and 1.3 RNAs at about the same or a slightly lower rate than 1.0 RNA. Rho-factor-mediated termination of transcription behind genes 0.3, 0.7, and perhaps behind 1.0 is inferred from these data. The in vivo translational utilization of the individual T7 early-message species was found to vary by not more than a factor of 2.


Replication process of the parvovirus H-1 V. Isolation and characterization of temperature-sensitive H-1 mutants. Two temperature-sensitive mutants of the parvovirus H-1 have been isolated and characterized. These mutants are distinguishable by the immunofluorescent staining of cells infected by them and by the thermolability of their hemagglutinins. Under restrictive conditions both mutants synthesize a missense capsid protein defective in hemmaglutination. Synthesis of the viral DNA strand was shown to be diminished for both wild-type virus and these mutants at the restrictive temperature of 39.5 C, but the mutants were more defective than the wild type at the less restrictive temperature of 38 C. Replicative form DNA replication was not decreased in these mutants. It is proposed that H-1 requires one of the capsid proteins for the synthesis of the single-stranded progeny DNA.


Purification and properties of bacteriophage phi X 174 gene D product. Bacteriophage phi X 174 gene D product, a protein required for single-stranded DNA synthesis by the phage, has been purified to near homogeneity. The protein is very abundant; approximately 10(5) monomers are present per infected cell when lysis is delayed. The protein has a monomer molecular weight of 15,200 and is normally a tetramer; however, it can form very large aggregates at high concentrations. Amino acid analysis shows an excess of arginine over lysine and a relatively high number of nonpolar residues. The protein carries a net negative charge at neutral pH. The first eight amino acids of the protein sequence have been determined; they are Ser-Gln-Val-Thr-Glu-Gln-Arg-Val. The carboxy-terminal residue is methionine. The protein has not yet been shown to bind directly to any single-stranded DNA; it does not adsorb to denatured calf thymus DNA-cellulose.


In vitro replication of cowpea mosaic virus RNA. II. Solubilization of membrane-bound replicase and the partial purification of the solubilized enzyme. A method for the solubilization of membrane-bound Cowpea mosaic virus RNA replicase has been developed by bypassing the use of detergents. Solubilization has been achieved by washing the 31,000 x g-pellet containing the bound replicase with a Mg2+-deficient buffer. This procedure had several advantages as compared to treatments with nonionic or ionic detergents: (i) the solubilized enzyme was stable at 4 C, (ii) more than 80% of the replicase could be solubilized without loss of total enzyme activity, (iii) the replicase was rather selectively released resulting in a two- to threefold increase in specific activity per se, and (iv) most of the green color from chloroplast fragments present in the crude replicase fraction remained membrane bound resulting in only slightly colored preparations of solubilized enzyme. The solubilized replicase has been further purified by DEAE-Bio Gel column chromatography. RNA synthesis directed by the DEAE-purified enzyme was template dependent and proceeded at a linear rate for at least 9 h.


Biochemical, biophysical, and biological properties of densonucleosis virus. I. Structural proteins. The polypeptide composition of highly purified densonucleosis virus was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The viral proteins showed a different behavior in sodium dodecyl sulfate-gels in comparison with the marker proteins. Therefore, the molecular weights were estimated by analyzing the retardation of the electrophoretic mobility of these proteins in gels with increasing polyacrylamide concentrations. Four structural proteins with molecular weights of 49,000, 58,500, 69,000, and 98,000 were found, ant they were designated p49, p59, p69, and p98, respectively. There are several indications that p98 is a dimer of p49. The relative quantity of the structural proteins in a virion suggests that at least p49 (accounting for +/-70% of total protein mass) is a capsid protein and that there will be 12 capsomers per virion.


Red system of bacteriophage  lambda complements the growth of a bacteriophage T1 gene 4 mutant. The ability of phage lambda to complement the growth of T1am23, a T1 gene 4 mutant with a DNA arrest phenotype, has been shown to require both lambda Red functions, redX and redB. lambdagam function, however, is not required. Therefore, the lambda Red function can substitute for T1 gene 4 function. However, T1+ does not substitute for lambda Red in allowing lambda to grow in a polA host.


Gene 32 protein of bacteriophage T4 moderates the activities of the T4 gene 46/47-controlled nuclease and of the Escherichia coli RecBC nuclease in vivo. Genes 46 and 47 of phage T4 control a nuclease that is required for genetic recombination and may act similarly to the Escherichia coli RecBC nuclease. In vivo, the nucleolytic activities of both of these nucleases must be moderated so that recombining DNA intermediates are not destroyed. We conclude from our present experiments that the phage T4 gene 32 protein, specifically its C-terminal domain, participates in such moderation. We have investigated DNA degradation in different gene 32 and gene 32/46 mutants under conditions that are completely restrictive for progeny production in all the mutants. Under these conditions, DNA of those gene 32 mutants in which the C-terminal domain of the protein is not synthesized or is modified is degraded to acid-soluble material. T4 gene 46 or E. coli recB mutations reduce such degradation; together they abolish it completely. By contrast, single gene 32 mutants which produce an unaltered C-terminal domain show little or no degradation of their DNA. Residual protection against nucleases is unrelated to residual primary DNA replication or to overproduction of the mutant peptides in the different gene 32 mutants.


Process of infection with bacteriophage phi X 174 XXXVIII. Replication of phi chi 174 replicative form in vivo. The replication of bacteriophage phi X 174 replicative-form DNA has been studied by structural analysis of pulse-labeled replicative-intermediate molecules. Such intermediates were identified by pulse-labeling with [13H]thymidine and separated into four major fractions (A, B, C, and D) in a propidium diiodide-cesium chloride buoyand density gradient. Sedimentation analysis of each of these fractions suggests the following features of phi X replicative-form DNA replication in vivo. (i) At the end of one cycle of replication, one daughter replicative form (RFII) contains a nascent plus (+) strand of the unit viral length, and the other daughter RFII contains small fragments of nascent minus (-) strand. (ii) Asymmetry is also associated with production of the first supercoiled RFI after addition of pulse label in that only the minus strand becomes radioactive. (iii) A supercoiled DNA (RFI') seems to occur in vivo. This DNA is observed at a position of greater density in a propidium diiodide-cesium chloride buoyant density gradient than normal RFI. (iv) A novel DNA component is observed, at a density greater than RFI, which releases, in alkali, a plus strand longer (1.5 to 1.7 times) than the unit viral length. These results are discussed in terms of the possible sequence of events in phi X 174 replicative-form replication in vivo.


Late events in T4 bacteriophage production. I. Late DNA replication is primarily exponential. The possibility of a switch in the mechanism of T4 DNA replication, from an exponential-bidirectional mode at early stages to a nonexponential (rolling circle) mode at later stages of phage development, has been investigated. The conclusion that DNA replication does not involve such a change in mechanism for the majority of replicating molecules is based on the analysis of the clonal distribution of mutants specifically induced at late times after infection. The clonal distribution of mutants, induced by adding 5-bromodeoxyuridine to infected cells at a time when 100 phage equivalents of DNA had accumulated, fits the pattern predicted by exponential replication.


Late events in T4 bacteriophage production. II. Giant bacteriophages contain concatemers generated by recombination. Analysis of "giant" phage, which package concatenated DNA into their capsids, shows that they are predominantly heterozygous. The results are compatible with the hypothesis that concatemers are generated by recombination.


Multiplication of parvovirus LuIII in a synchronized culture system. III. Replication of viral DNA. The replication of the single-stranded DNA (ssDNA) of parvovirus LuIII was studied in synchronized HeLa cells. After infection of the cells in early S phase, synthesis of a replicative form (RF) DNA became detectable as early as 9 h postinfection, i.e., after display of the cellular helper function(s) indispensable for the replication of LuIII virus. According to digestion with nuclease S1, hybridization studies, and electron microscopy, RF DNA is a linear, double-stranded molecule comparable in length to mature ssDNA. It sedimented around 15S in neutral solution and banded at 1.714 g/ml in CsCl. Moreover, replication of LuIII DNA obviously includes a further replicative intermediate DNA which sedimented in front of RF DNA and bore single-stranded side-chains. Newly synthesized DNA disappeared from pools containing both RF DNA and replicative intermediate DNA within 5 min and reappeared in progeny virions only after 15 min. Intranuclear accumulation of significant amounts of progeny ssDNA could not be detected. It was postulated, therefore, that newly synthesized ssDNA is immediately enclosed in a stable maturation complex and resists extraction by the method of Hirt (1967).


In vivo and in vitro synthesis of adenovirus type 2 early proteins. The synthesis of adenovirus type 2 (Ad2)-induced early polypeptides was examined in vivo and in vitro by a combination of sodium dodecyl sulfate-polyacrylamide gel electrophoresis alone and specific immunoprecipitation followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Analysis of total [35S]methionine-labeled polypeptides synthesized in vivo at 3 h postinfection allowed us to detect in infected cells at lease 13 distinct polypeptides that are either absent or less conspicuous in extracts from mock-infected cells. These Ad2-induced early polypeptides have molecular weights ranging from 72 x 10(3) to 10.5 x 10(3) and have accordingly been designated as E72K to E10.5K. Nine of the in vivo synthesized early polypeptides can be precipitated specifically from infected cell extracts by antisera with specificity against early adenovirus proteins. In vitro translation of mRNA extracted from mock-infected cells and from Ad2-infected cells was carried out in preincubated Ehrlich ascites cell extracts. All the early Ad2-induced polypeptides identified in the extracts from infected cells labeled in vivo were also detected among the polypeptides immunoprecipitated specifically from the in vitro reaction mixtures programmed by RNA extracted at 4 h postinfection from Ad2-infected cells.


Differences in RNA patterns of influenza A viruses. Analysis of the segmented RNAs of influenza A viruses by electrophoresis on polyacrylamide urea slab gels has provided a method for sharper resolution of the number and migration rates of different segments than previously has been possible. Using this system, the RNA genome of influenza A/WSN (HON1) virus can be separated into seven to nine separate bands, depending on whether virus is obtained after high or low multiplicity of infection, and the genome of influenza A/PR/8 (HON1) virus can be resolved into eight bands, six of which migrate differently from comparable RNA bands of WSN virus. Comparision of the RNA patterns produced by influenza A/PR/8 (HON1) and A/England/42/72 (H8n2) virus also reveals major differences in migration speeds of different bands, and analysis of the RNAs of the RNAs of an HON2 recombinant virus derived from these two strains permits the identification of RNA segments which have been derived from one particular parent. By extension of these techniques, it may be possible to define which RNA segment codes for each viral protein and to analyze recombinant strains to identify which genes have been derived from each of its parents.


Demonstration of subpopulations of Dane particles. Two populations of Dane particles were isolated from the plasma of individuals carrying hepatitis B surface antigen. These populations had densities in CsCl of 1.22 and 1.20 g/ml. Endogenous DNA polymerase activity was found to be associated only with the heavier of these two populations. Using a positive stain, electron microscopic examination of these particles suggested that the heavier the particle contained nucleic acid in its core whereas the lighter particle appeared empty. Cores isolated from Dane particles with densities of 1.22 and 1.20 g/ml banded in CsCl at densities of 1.36 and 1.30 g/ml, respectively. Endogenous DNA polymerase activity was associated only with the higher density core particles.


Head morphologies in bacteriophage T4 head and internal protein mutant infections. Escherichia coli infected with phage T4 mutants defective in synthesis of the three major internal proteins found in the phage head, IPI-, IPII-, IPIII-, or IP degrees (lacking all three) were examined in the electron microscope for head formation. Infection with IPI- or IPII- does not appear to induce increased aberrant head formation, whereas IPII- or IP degrees infections result in production of polyheads and viable phage. Multiple mutants of the early head formation genes 20, 21, 22, 23, 24, 31, 40 and IP degrees were constructed. Combination with IP degrees increases polyhead formation when head formation is not blocked at a more defective stage but results in a qualitative shift to lump formation in association with gene 22 mutants. Thin-sectioning studies show morphologically similar cores in amber 21 and 21am IP degrees tau particles. These morphological observations, genetic evidence for interaction between ts mutants in gene 22 and the IP mutants, and analysis of the protein composition of tau particles further support the idea that p22 and the internal proteins form an unstable assembly core necessary for an early stage of head formation (M. K. Showe and L. W. Black, 1973).


Newcastle disease virus-specific RNA: poly(A)-containing and poly(A)-deficient transcripts as revealed by chromatography on poly(U)-sepharose. Total [3H]uridine-labeled, virus-specific RNA from Newcastle disease virus-infected cells was fractionated by poly(U)-sepharose chromatography and analyzed by rate zonal gradient centrifugation. The sedimentation pattern of both eluted and nonadsorbed RNA resembled that of the total RNA. However, nonadsorbed RNA was enriched in 50S material, ant its 18S peak was broader and slightly shifted towards the top of the gradient. Poly(U-sepharose chromatography of isolated 18S RNA and 24S RNA resulted in the separation of poly(A)-containing RNA and poly(A)-deficient RNA. In the former the percentage of adenosine content represented by poly(A) sequences was estimated as 10 to 12% (for 18S RNA) or approximately 6.0% (for 24S RNA). The size of poly(A) fragments as measured by their sedimentation rate was the same for 18S and 24S RNA. Polyacrylamide gel electrophoresis of poly(A)-containing RNA revealed a characteristic pattern closely resembling the pattern of nonchromatographed 18S and 24S RNA. The pattern of poly(A)-deficient RNA was heterogenous, and for 18S RNA it shifted towards the anode. The possible origin of poly(A)-deficient transcripts is discussed.


Isolation and characterization of an extremely basic protein from adenovirus type 5. By starch-gel electrophoresis and a staining method that is highly sensitive for argininyl residues, adenovirus type 5 was found to contain two minor basic polypeptides of extreme cathodic mobility in addition to the two known core proteins. The fastest-migrating polypeptide, named mu protein, and the second fastest polypeptide are found in adenovirions and virus-infected KB cells but not in top components or in uninfected cells. The top components and infected cells contain an additional basic polypeptide, presumably P-VII, that migrates slightly slower than polypeptide VII. None of the basic polypeptides of adenovirions was electrophoretically identical to the host histone. The basic proteins of adenovirions were purified by urea phosphocellulose column chromatography and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The two minor basic core proteins, mu and another component, have similar mobilities in sodium dodecyl sulfate-polyacrylamide gels as a complex of polypeptides X-XII. After further purification on a Sephadex G-75 column, the mu protein was found to have a molecular weight of about 4,000. Amino acid analysis showed that the mu protein lacks tryptophan and 69% of the total amino acid residues are basic, that is, 54% arginine, 13% histidine, and 2% lysine. Only eight amino acids seem to contribute to make the mu polypeptide. There are 125 copies of the mu polypeptide per 1,000 copies of polypeptide VII in a virion.


Recombination between temperature-sensitive and deletion mutants of reovirus. In standard pairwise crosses there was no detectable recombination between defective reovirus lacking the largest genomic segment and prototypes of the seven known classes of ts mutants. However, in such crosses between R2A (201) and the various prototypes frequencies of ts+ recombinants between 2.6 and 6.1% were observed, as others have found (Fields, 1971; Fields and Joklik, 1969). An infectious center assay was devised to measure recombination in this system, and it was found that all mixedly infected cells gave rise to ts+ recombinants in crosses between prototype ts mutants, but no recombination was detectable when the defective virus was crossed with three different ts mutants. The ts mutation of mutant R2A (201) was efficiently rescued when crossed with UV-inactivated wild-type virus but not when crossed with UV-inactivated defective virus. It is concluded from these various experiments that if there is any recombination between these defective reovirions and any known class of ts mutants it is too low to be measured by methods presently available. The kinetics of recombination were measured in cells mixedly infected with R2A (201) and R2B (352) mutants. At the earliest time progeny virus could be found in the cells the frequency of ts+ recombinants was 4.5%, and this frequency remained unchanged despite a subsequent 1,000-fold increase in progeny virus.


Isolation of the dengue virus envelope glycoprotein from membranes of infected cells by concanavalin A affinity chromatography. The membranes isolated from type 2 dengue virus-infected BHK-21/15 cells contain three glycosylated virus-specified proteins; one corresponds to the virion envelope glycoprotein, V-3, whereas the other two are nonstructural virus-specified proteins, NV-2 and NV-3. A combination of two nonionic detergents, Nonidet P-40 and Triton X-305, solubilized greater than or equal to 80% of the membrane-bound protein and the majority of the type 2 dengue virus complement-fixing antigens. The soluble material was adsorbed by concanavalin A-Sepharose in the presence of the nonionic detergents, which were subsequently removed by washing with deoxycholate-containing buffer. Finally, the bound glycoprotein was eluted by the addition of alpha-methyl glucopyranoside. V-3 was the only virus-specified protein in the alpha-methyl glucopyranoside eluate. The V-3-containing fraction did not cross-react with antisera against other selected Flaviviruses in the complement fixation tests. The V-3 contained in the isolated fraction differed from the parent membrane-bound V-3 in two interesting, and as yet unexplained, ways: (i) it exhibited hemagglutinating activity similar to that of the infectious virus, but (ii) it did not block the action of neutralizing antibody.


Viral transcription in KB cells infected by temperature-sensitive "early" mutants of adenovirus type 5. RNA:DNA hybridization was used to study the synthesis of viral RNA in two DNA-minus, temperature-sensitive mutants of type 5 adenovirus (H5ts125 and H5ts149) belonging to two different, non-overlapping complementation groups. Hybridization competition analysis showed that both mutants transcribed all early gene sequences at the restrictive temperature (41 C). In mutant-infected cells at 41 C, the rate of viral transcription was similar to the rate of early RNA synthesis in wild-type virus infection and was dependent on the multiplicity of infection; little or no late transcription was detected. The shutoff of class I early RNA transcription was shown to be a late function during wild-type virus infection and did not occur at 41 C in mutant-infected cells. When mutant-infected cells were incubated at the permissive temperature (32 C) for 25 h and then shifted to 41 C, the rate of viral DNA synthesis decreased rapidly for H5ts125 and slowly for H5ts149. However, the rate of viral transcription remained unchanged in H5ts125-infected cells for at least 3 h after the temperature shift; although the synthesis of viral DNA had stopped by this time, the synthesis of late viral RNA sequences continued.


Introduction of interrupted secondary structure in supercoiled DNA as a function of superhelix density: consideration of hairpin structures in superhelical DNA. PM2 DNA was prepared with different superhelical densities (sigma) in order to examine the relationship betweenn supercoiling and the occurrence of a region(s) of unpaired bases in this DNA. A previous study showed that CH3HgOH reacts with native superhelical PM2 DNA more rapidly than the nicked form II. This evaluation of binding, monitored through the change of sedimentation velocity, was repeated on PM2 DNA I with different superhelical densities. Early binding is detected by an increase in sedimentation velocity and occurs with molecules with sigma' values betwee -0.025 and -0.037. The conversion of form I to form II with the single-strand-specific endonuclease from Neurospora crassa also occurs above a sigma value of -0.025. This data strongly supports the view that supercoiling produces interrupted secondary structure. The question whether the interrupted regions remain single stranded in character or form small intrastrand hairpin regions is considered by examining which model best fits the CH3HgOH- induced sedimentation velocity changes and the standard sedimentation velocity versus the superhelical density curve for the in vitro made DNAs. The hairpin model offers the most satisfactory explanations for all the results of this and previous studies.


Process of infection with bacteriophage phi chi 174. XL. Viral DNA replication of phi chi 174 mutants blocked in progeny single-stranded DNA synthesis. Mutation in several different cistrons of bacteriophage phi chi 174 blocks net progeny single-stranded DNA synthesis at the late period of infection (15). For the study of the functions of these cistrons in single-stranded DNA synthesis, asymmetric replication of replicative form DNA was examined at the late period of infection with amber mutants of these cistrons. While the normal, rapid process of asymmetric single-stranded viral DNA synthesis is blocked at the late period of these mutant infections, an asymmetric synthesis of the viral strand of replicative-form DNA is observed in this period, though at a reduced level, together with degradation of prelabeled viral strand. Some intermediate replicative-form molecules were also detected. Asymmetric synthesis of the viral strand of replicative-form DNA at the late period of phi chi infection is completely inhibited in the presence of a low concentration (35mug/ml) of chloramphenicol (which also blocks net single-stranded viral DNA synthesis). These results are discussed in terms of the possible role of the specific viral proteins for normal single-stranded DNA synthesis.


Multiple origins and circular structures in replicating T5 bacteriophage DNA. Replicating T5 phage DNA was gently isolated using NaI density gradient centrifugation and examined by electron microscopy. At the beginning of phage DNA synthesis, linear unit-length T5 DNA molecules containing from one to four replicating "eye-loops" were consistently observed. Replication in these molecules was found to proceed bidirectionally from multiple, internal origins. A primary origin of replication is located near the center of the T5 genome, which does not coincide with the location of any of the nicks (single-strand breaks) found in mature T5 DNA. The initiation of replication at the various origins within an individual molecule does not appear to follow any definite temporal sequence. At later times in the infection, we have observed a significant number of circular T5 DNA molecules-both replicating and nonreplicating-whose average circumference is approximately the length of mature T5 DNA minus the terminal redundancy. The replicating circular molecules appear to be either in a theta configuration, a sigma configuration with the tails all being less than the length of the circle, or a combination of theta and sigma forms.


Bidirectional replication of adenovirus type 2 DNA. After short periods of labeling with [3H]thymidine, recently completed adenovirus DNA molecules were isolated and cleaved with restriction endonucleases. The strands (heavy and light) of most of the restriction endonuclease fragments were separated. The pattern of labeling clearly shows an asymmetry of radioactivity on the isolated strands of each restriction endonuclease piece. The data is consistent with replication proceeding in the 5' to 3' direction on each strand. Thus, there is an initiation point placed at or near each end of the molecule.


Comparative electrophoresis of the 18-22S RNAs of Newcastle disease virus. Between 80 and 90% of the 18-22S Newcastle disease virus intracellular RNA molecules contain poly(A) sequences. Electrophoresis of the 18S RNA in formamide-polyacrylamide gels resolves five species resolved by electrophoresis in aqueous gels. Thus, these five RNA species are probably unique size classes of RNA and not different conformations of the same RNAs. They are of sufficient size to code for the five smaller Newcastle disease virus proteins, and their combined molecular weights represent 60% of the viral genome-a value identical to that obtained by annealing 18-22S RNA with genome RNA. Formamide or heat treatment of the 22S RNA converts most of it into species with migration rates similar to those of the 18S species. Thus, the 22S RNA may not contain unique RNA species.


Transcriptase activity and genome composition of defective influenza virus. Eight genome RNA segments are present in both normal and von Magnustype influenza virus preparations and all species are transcribed by the virion-associated polymerase. Although the RNA polymerase activity and the amount of the three largest RNA segments are reduced in defective influenza virus preparations, these reductions do not appear to be great enough to account for the much greater loss of infectivity.


Proteins solubilized from frog virus 3 particles: effect on transcription. The treatment of KB cells with viral proteins solubilized from frog virus 3 particles (SVE) induced a rapid shutoff of host RNA synthesis. The RNA polymerase activities of SVE-treated cells were drastically depressed, corresonding, at least for RNA polymerase B, to a decrease in the number of enzyme molecules. In vitro, SVE had no direct effect on RNA polymerases but was capable of binding with calf thymus DNA to form an SVE-DNA complex modifying the template capacity. The effect of SVE on a transcription system consisting of cell lysates would suggest that cytoplasmic factors are necessary for expression of the inhibitory capacity of SVE.


Regulated transcription of the genomes of defective virions and temperature-sensitive mutants of reovirus. Defective reovirus, which lacks the largest (L1) of the 10 double-stranded (ds) RNA genomic segments, attaches to L cells and is uncoated in the same way as reovirus. The defective genome does not replicate in the cells, but it is transcribed. During the first 5 h after infection, three of the genomic segments, M3, S3, and S4, are more frequently transcribed than the remaining six segments. During the succeeding 5 h, there is a transition to a situation in which all nine segments are transcribed at the same relative frequencies. Since the class C ts mutation has been allocated to the L1 segment (Spandidos and Graham, 1975) the transcription of the C mutant genome was investigated in cells infected with it at the nonpermissive temperature, at which the parental genome does not replicate. Genomic segments L1, M3, S3, and S4 are predominantly transcribed at early times, and later all 10 segments are transcribed with the same relative frequencies. Transcription of the defective viral genome and the C mutant genome is therefore regulated in the same way as previously found for wild-type virus (Nonoyama, Millward, and Graham, 1974), and the regulation is independent of genome replication. Apparently the L1 segment function is involved in dsRNA synthesis but not in regulating the early to late transcription. It is suggested that a cellular repressor may be involved in this regulation and that derepression might be effected by one of the early viral gene products. Virion transcriptase activity was studied in vitro with cores prepared by chymotrypsin digestion of purified defective and standard virions. For both genomes the relative frequencies of transcription of the dsRNA segments are inversely proportional to their molecular weights. These results can be accounted for in a model that postulates each segment to be transcribed independently of the other. The same model with certain restrictions can describe the in vivo transcription of the viral genome.


Functional half-lives of bacteriophage m13 gene 5 and gene 8 messages. The half-lives of the M13 gene 5 and gene 8 messages were determined by measuring the decay in the rate of synthesis of the gene 5 and gene 8 proteins after inhibition of new RNA chain initiations with rifampin. The gene 5 and gene 8 messages decay with half-lives of approximately 2.5 and 5 min, respectively. We found no evidence of a functional M13 message with a half-life as long as that reported for hybridizable mRNA.


Traumatic renal artery occlusion: 21 cases. Sixteen cases of unilateral and 5 cases of bilateral traumatic renal artery occlusion caused by avulsion or thrombosis are presented. The injury typically follows automobile-pedestrian accidents to young male subjects. Associated extrarenal injuries are usual but non-pedicle renal injury is infrequent. Suspicion of the unilateral injury depends upon recognition of absence of visible excretion at urography. The clue to bilateral occlusion is anuria. Diagnosis is confirmed by urgent arteriography. Hematuria was absent in 24 per cent and the injury was missed at laparotomy in 29 per cent of the initial explorations. Renal function was salvaged by arterial reconstruction in 2 cases, 12 hours and 5 months after injury respectively. Hypertension developed in 50 per cent of the cases and was generally mild.


Cell-mediated immunity in renal cell carcinoma-preliminary report. The microcytotoxicity test was used in 8 cases post-nephrectomy for renal cell carcinoma to stage the disease clinically and to evaluate immunologically. Half of the patients had stage IV disease and half were believed to be cured clinically. Cell-mediated immunity and serum blocking factors were found in all patients with known metastases. However, cellular immunity was not found in 3 of the 4 remaining patients and serum blocking factors were also not noted in the 2 clinically cured patients who were tested. These findings are compatible with the facts that cell-mediated immunity and serum blocking factors are found when there is a grossly discernible antigenic source, that serum blocking factors are lost in patients who are clinically free of tumor and that in some patients who are clinically cured with a possible total loss of antigenic presence there is a disappearance of significant cell-mediated immunity.


In vitro assessment of cell-mediated immunity in patients with renal cell carcinoma. We evaluated 25 patients with renal cell carcinoma for cell-mediated immunity using the microtoxicity assay. Normal controls frequently exhibited significant cell inhibition. The mean percentage cell inhibition in patients after nephrectomy was significantly higher than in patients before nephrectomy and significantly higher than normal male subjects. No other correlation was found with respect to the stage of the disease. The incidence of significant autoblocking activity was noted more commonly in patients after nephrectomy than in patients before nephrectomy.


Immune cytolysis of human renal carcinoma mediated by xenogeneic immune ribonucleic acid. With a microcytotoxicity assay it was shown that normal, non-immune human lymphocytes were converted to effector cells specifically cytotoxic to human renal carcinoma cells after incubation with xenogeneic immune ribonucleic acids. The ribonucleic acid was extracted from the lymphoid tissues of sheep that had been immunized with human renal carcinoma tissue. Lymphocytes incubated without ribonucleic acid from or with ribonucleic acid sheep immunized with Freund's adjuvant alone did not increase cytotoxicity. Immunotherapy with immune ribonucleic acid increased cytotoxic activity of lymphocytes from a patient with metastatic renal carcinoma. The microcytotoxicity assay may be a useful method to assessing the cellular immune response in patients receiving immunotherapy and seems to correlate with their clinical course.


The interaction of host immunocompetence and tumor aggressiveness in superficial bladder carcinoma. The results of our studies suggest that in the natural history of bladder cancer intrinsic tumor aggressiveness as measured by loss of normal antigenic activity may be more relevant than intact host defenses as measured by an intact cell-mediated immune response. Our contention that tumor aggressiveness may be the more important variable fits in with the occasional success in cancer patients treated with chemotherapeutic agents that act by altering the genetic material of rapidly dividing tumor cells. Our data suggest that therapy directed at bolstering the host's immune system may not be as effective as therapy directed against molecular events within the cancer cells themselves. Unfortunately, presently available non-surgical modalities do not discriminate between aggressor (tumor) and defender (lymphocyte) cells.


Prognostic significance of regional lymph node histology in cancer of the bladder. The regional lymph nodes from 47 patients with carcinoma of the bladder who had undergone radical cystectomy and bilateral pelvic lymphadenectomy were classified into 3 histologic patterns that correlated with immunologic function. Lymph nodes were designated as stimulated if they exhibited prominent germinal centers (B cell proliferation) and expansion of the deep cortex (T cell proliferation), depleted if they appeared markedly hypocellular and fibrotic, and unstimulated if they resembled a normal resting lymph node. Correlation of the histologic pattern with the extent of disease revealed that patients whose nodes appeared stimulated had fewer metastases (p less than 0.05) than those with either unstimulated or unstimulated combined with a depleted pattern. A markedly improved 5-year survival rate was seen in patients with a stimulated pattern (p less than 0.0001) compared to those patients who exhibited a depleted and/or unstimulated lymph node pattern. The survival advantage related to the stimulated pattern was observed primarily among patients with advanced disease. It is suggested that stimulated nodes reflect proliferation of T and B lymphocytes engaged in cell-mediated and humoral immune responses to the bladder tumor and that this favorably influenced survival in those patients. Patients whose lymph nodes showed a depleted pattern fared poorly despite the extent of the disease and those with an unstimulated pattern were intermediate in survival. A depleted pattern may represent a state of local immune paralysis, exhaustion of the draining lymph nodes as a result of exposure to excess tumor-derived products such as antigen or toxic substances or simply an atrophic node incapable of response. In the absence of a local immune response such patients might be expected to do poorly. These results suggest that morphologic evaluation of the lymph nodes regional to bladder cancer may provide a clue to their immunologic function and a more accurate guide to prognosis of patients with this neoplasm.


Male incontinence: relationship of physiology to surgery. After prostatectomy it is the passive continence mechanism that maintains continence. Since this mechanism is less effective when the structures are not in the normal elevated position, perineal continence operations are probably successful in proportion to the mobility of the sub-prostatic structures and the ability to keep them elevated.


Experience with the Small-Carrion penile prosthesis. The Small-Carrion prosthesis has been implanted successfully in 14 of 15 patients. These prostheses are implanted through a dorsal midline penile incision which simplifies the procedure. The perineal approach is preferred for keloid formers and paraplegics who will use a condom catheter. Operative failure may be decreased by paying particular attention to the size of prosthesis implanted and the positioning of its distal tip.


Experience with the Small-Carrion penile prosthesis in the treatment of organic impotence. Since certain animals are endowed with an os penis the concept of using penile implants was a natural development. Herein we report on our experience with the Small-Carrion penile prosthesis in 23 patients. The surgical technique is described as well as several examples of postoperative results. The major complication encountered was infection. All patients were able to accomplish coitus without significant difficulty. Proper patient selection and the attitude of the sexual partner constitute the major factors in the ultimate success or failure of the procedure.


A study of meiotic preparation of human spermatocytes and their relationship to infertility. Although our experience is based on a small series of 50 cases we believe that several points can be made. Meiotic preparations can be performed in any competent cytogenetic laboratory. These preparations appear to yield more specific information as to the site of arrest and may be more reliable than the static testicular biopsy to appraise the true status of spermatogenesis. With these facts in mind a more accurate prognosis and therapeutic regimen can be realized.


Renal abscess: a changing concept.

Renal abscess: a changing concept. Gram-negative organisms are not the most prevalent bacterial species found in renal abscesses. We postulate an ascending pathway for establishment of gram-negative abscesses. Voiding cystourethrography should be used in search of reflux. Surgical treatment remains the standard but early diagnosis may increase the effectiveness of parenteral antibiotics as definitive therapy.


Excretory urographic demonstration of ureteral displacement by sigmoid fecal impaction simulating retroperitoneal tumor. Two cases of curvilinear lateral ureteral displacement in children with abdominal masses are reported. The masses proved to be sigmoid fecal impactions. This possibility must be considered before embarking on radiographic or surgical evaluation of possible retroperitoneal neoplasm.


Intrarenal reflux and renal damage.

Intrarenal reflux and renal damage. The retrograde flow of dye from the calices into the collecting tubules (intrarenal reflux) seen during cystrography in children and its effect on the kidneys were evaluated. A retrospective analysis was made of 175 patients with excretory urograms revealing cortical atrophy. Of these patients 68 per cent had cystograms revealing vesicoureteral reflux. Intrarenal reflux was seen in 8 patients less than 6 years old, an incidence of 13.5 per cent in that age group. There was a high correlation, 7 of 8 or 87.5 per cent, between the areas of intrarenal reflux, and associated renal cortical atrophy and calicectasis. Intrarenal reflux provides a pathway by which bacteria and hydrostatic pressure may produce injury to the renal parenchyma. The presence of intrarenal reflux is an absolute indication for ureteroneocystostomy.


Urinary diversion for neurogenic bladder in children. The fate of the upper urinary tracts after urinary diversion will determine its place in the management of neurogenic and congenital bladder lesions. In this study there was an over-all 13 per cent deterioration rate in the upper tracts after ileac and colonic diversions. The status of the upper tracts at the time of diversion was believed to be a significant factor in the prognosis.


Scrotal swelling in the Schönlein-Henoch syndrome. Testicular and scrotal hemorrhage is uncommon in the Schönlein-Henoch syndrome. The diagnosis of testicular torsion is difficult to make when it is complicated by vasculitis of the testis. Since both conditions can occur simultaneously, torsion of the testis must be considered and surgical exploration performed whenever acute testicular pain and swelling occur during the course of the Schönlein-Henoch syndrome. Two patients with this syndrome as well as scrotal and testicular involvement are described.


Diagnostic features of tumors of the lower thoracic sympathetic chain. Four cases of sympathetic neural tumors of the most inferior portion of the thoracic sympathetic chain are presented. The location of each tumor was such that a primary adrenal tumor might have been considered. However, each tumor was associated with erosion of the ipsilateral lower ribs or vertebral pedicles. Such radiologic findings indicate a primary posterior mediastinal tumor and primary exploration should be transthoracic.


Combined adrenorenal fusion and adrenohepatic adhesion: a case report with review of the literature and discussion of pathogenesis. The second case of combined adrenorenal fusion and adrenohepatic adhesion on the right side is reported. It is hypothesized that the basic lesion underlying this innocuous anomaly rests in the periadrenal mesenchyme. In the case of fusion of adrenal with kidney or liver the mesenchymal defect causes 1) retardation of capsule formation with parenchymal mixing and 2) failure of local differentiation into fetal and later adult fat cells. In the case of adhesion of adrenal to kidney or liver there is no retardation of capsule formation with parenchymal mixing but only failure to differentiate locally into fat cells. Consequently, there is no physical separation of these organs by interposition of fat cells and there is variable party-wall sharing of the capsule.


Simple renal cyst and high renin hypertension cured by cyst decompression. A case of renin elevated hypertension cured by cyst decompression is presented. The proposed mechanism is segmental renal ischemia produced by the cyst. Renal cyst evaluation should include renin studies if hypertension is present.


Transcatheter biopsy of renal carcinoma invading the inferior vena cava. Transcatheter biopsy is presented as an alternate method of obtaining a histopathologic diagnosis in cases with evidence of direct neoplastic intravascular involvement and when conventional biopsy methods may be contraindicated.


Ureteral obstruction and pyelonephritis caused by an inguinal hernia: report of a case. An elderly man had pyelonephritis and sepsis owing to ureteral obstruction. Retrograde pyelography showed entrapment of the right ureter in an inguinal hernia. This condition, which may be congenital or acquired, should be considered before herniorrhapy is performed and as a possible cause of ureteral obstruction.


Pyeloureteral filling defects associated with systemic anticoagulation: a case report. The etiology of pyeloureteritis cystica has long been attributed to chronic infection and inflammation. A case is presented that is unique in that the acute onset and the rapid resolution of pyeloureteral filling defects in this patient were documented by radiography. There is no evidence of antecedent or concurrent infection in this patient. The disease occurred subsequent to the initiation of heparin therapy for suspected pelvic thrombophlebitis and cleared rapidly subsequent to its discontinuation. The rate of resolution of the radiographic findings may be helpful in distinguishing between true pyeloureteritis cystica and submucosal hemorrhage.


Carcinoma of the urachus: its demonstration by pelvic pneumography. Carcinoma of the urachus is an uncommon neoplasm that usually presents a diagnostic problem. A case of urachal carcinoma visualized by pelvic pneumography is reported. The clinical and radiological features of this lesion are briefly discussed.


Genitourinary tract anomalies in lobster claw syndrome. A child with ectrodactyly or lobster claw syndrome and bilateral ureterovesical junction obstruction is described. The embryogenesis of the kidney and the limb buds suggest that a close association exists between these 2 conditions. Therefore, it is important to investigate the urinary tract in all patients with limb deformities.


Lymphocystis disease in the winter flounder, Pseudopleuronectes americanus. Nodular lesions on the fins of winter flounder, Pseudopleuronemphocystis disease. Hypertrophied, encapsulated connective tissue cells contained cytoplasmic inclusions composed of icosahedral virus particles. The winter flounder is a new host for lymphocystis disease and is the second flatfish in the western North Atlantic to have the disease.


A relationship between avian carcasses and living invertebrates in the epizootiology of avian botulism. A survey of the sources of Clostridium botulinum type C toxin possibly utilized as food by aquatic birds in an epizootic area of avian botulism in northern Utah showed that living aquatic and terrestrial invertebrates normally found in close association with dead, decomposing birds commonly carried the toxin. Of 461 samples associated with 21 species of avian carcasses, 198 were toxin-positive. Invertebrate species not normally scavengers of vertebrate tissues were less commonly and less highly toxic, particularly when captured 30 cm or more from a carcass; six of 237 samples of such aquatic invertebrates low-level toxin. Of the species tested, blow fly larvae (Calliphoridae) were the most consistently and highly toxic, although others, particularly adult and larval stages of several species of beetles (Coleoptera), contained toxin at levels probably significant in the epizootiology of the disease. An estimated 0.05 to 0.25 g of the most toxic fly larvae or 15 g of the most toxic beetles tested carried a mediam lethal dose for an adult mallard duck. Examination of stomach contents of aquatic birds dead of botulism showed that some had consumed invertebrates.


The effects of lead poisoning on various plasma constituents in the Canada goose. Plasma glucose, free fatty acid and uric acid levels were measured in lead-poisoned Canada geese (Branta canadensis). Although plasma glucose levels were only slightly elevated, uric acid was significantly higher and free fatty acids were significantly lower. Altered plasma levels were attributed to increased protein catabolism and perhaps renal disfunction. Plasma level of growth hormone and prolactin was assessed by radioimmunoassay. Growth hormone remained unchanged while prolactin was unusually high. The increased prolactin levels may reflect an effort to stabilize free fatty acids.


Infection and mortality in captive wild-trapped canvasback ducks. Fungal, bacterial and malarial infections, as well as malnutrition caused heavy mortality in a group of wild-trapped canvasbacks (Aythya valisineria) held in 10 X 3 X 2 m open-water pens. Deaths occurred between 21 and 158 days after confinement and were associated with infections of Aspergillus fumigatus, Escherichia coli, Staphylococcus sp., Streptococcus sp., Klebsiella sp., Enterobacter sp., and Plasmodium sp. Infection and mortality was believed to result from reduced resistance associated with confinement. Fourteen canvasbacks released onto large ponds survived throughout the period during which the penned birds died.


Fluorosis in black-tailed deer.

Fluorosis in black-tailed deer. Marked dental disfigurement and abnormal tooth wear patterns were observed in black-tailed deer (Odocoileus hemionus columbianus) taken from an area near an industrial fluoride source in northwestern Washington. Fluoride levels in the bones of these deer were from 10 to 35 times higher than levels in the bones of normal animals. These levels are similar to those associated with fluorosis of cattle.


Lesions associated with Orthohalarachne attenuata (Halarachnidae) in the northern fur seal (Callorhinus ursinus). In northern fur seals (Callorhinus ursinus) up to at least 4 years of age there is virtually 100% prevalence of infestation with the nasal mite Orthohalarachne attenuata. Although clinical observations and gross examination indicate that the condition is not serious, some erosion and inflammation of the nasal turbinates and nasopharynx were seen associated with mites in histological sections.


A trematode from the round window of an Atlantic Bottlenosed dolphin's ear. A trematode from the family Nasitrematidae Yamaguti 1951 was found adhered to the round window in the inner ear of a Bottlenosed Dolphin (Tursiops truncatus). The possibility that parasites could be responsible for changes in acoustic behavior and hearing loss is discussed.


Influence of rainfall on lungworm infections in bighorn sheep. From 1959 through 1968, lungs from 124 Rocky Mountain bighorn sheep (Ovis c. canadensis) from the Sun River herd in western Montana were examined for lungworm infections. All lungs were infected with Protostrongylus stilesi and 104 (84%) contained concurrent infections of P. stilesi and P. rushi. Significant correlations were observed between levels of lungworm infection and total rainfall during April, May, and June of each year. An explanation of this in terms of terrestrial snail (intermediate host) populations and a suggestion for the possible use of these data in developing a predictive model for forecasting lungworm levels for use in in bighorn sheep management are given.


Some observations on chronic leptospiral carrier state in gerbils experimentally infected with Leptospira grippotyphosa. Leptospiruria with persistent microscopic agglutinating antibody titers were maintained in gerbils that became carriers following experimental infection with Leptospira grippotyphosa, strain F 4397. Leptospires were isolated from kidneys of a gerbil which died 28 months after experimental inoculation.


Ixodes scapularis Say on white-tailed deer (Odocoileus virginianus) from Long Point, Ontario. Ixodes scapularis Say was the only species of tick found on white-tailed deer, Odocoileus virginianus, collected at Long Point, Ontario from October 1972 to August 1973. Adults were most abundant from September 1972 to April 1973. Larvae were found throughout the study period except during February. Nymphs were scarce during winter months but fairly common during spring and summer. Most adults were found on the neck and shoulders. Larvae occurred mainly on lower regions of the body and nymphs mainly on the head, shoulders, forelegs and brisket.


Some hematologic values of bison from five areas of the United States. Seventeen hematologic values of the American Bison (Bison bison) from five areas of the United States were determined using standard techniques. The means of the principal blood measurements for all bison were 10.08 +/- 1.43 million erythrocytes/mm3, 8.03 +/- 1.41 thousand leukocytes/mm3, 16.92 +/- 1.43 gm % hemoglobin and 47.11 +/- 4.06% hematocrit. There was a significant variation (P less than 0.05) among age groups of males for erythrocytes, neutrophils, lymphocytes and monocytes. However, no significant variation between female age groups or sexes was found for any of the blood cell values determined.


Blood changes in brook trout induced by infection with Aeromonas salmonicida. Furunculosis was induced in brook trout, Salvelinus fontinalis, by experimental inoculation with Aeromonas salmonicida. Total protein, hemoglobin, sialic acid, fatty acids, triglycerides, cholesterol, inorganic-phosphorus, acid-soluble phosphorus, and lipid-phosphorus decreased in the blood of the infected fish while amino acids, urea, total creatinine, ammonia, and glucose increased. Pyruvic acid, lactic acid, and ascorbic acid values showed no significant change.


Mycotic flora in the lower digestive tract of feral pigeons (Columba livia) in the El Paso, Texas area. Fourteen species of fungus were isolated from the lower digestive tract of 39 of 80 pigeons. Sixteen pigeons had concurrent isolations while two harbored three species. Fungi isolated were Allescheria boydii, Aspergillus spp., Candida krusei, Chrysosporium spp., Geotrichum candidum, Mucor spp., Paeciliomyces spp., Penicillium spp., Rhizopus spp., Rhodotorula spp., Scopulariopsis spp., Streptomyces spp., and Trichosporon cutaneum. There was no apparent evidence that these fungi were associated with clinical disease in any of the pigeons.


Renal capillariasis in the small Indian mongoose, Herpestes auropunctatus. A Capillaria sp. was recovered from the kidneys of 28 (93.3%) of 30 small Indian mongooses (Herpestes auropunctus) collected in St. Lucia, West Indies. The nematodes were embedded within distended pelvic fornices of the kidney and surrounded by accumulations of eggs. A chronic, low-level inflammation of the transitional epithelium was characterized by hyperplasia, giant cells surrounding embedded eggs and a plasmacytic infiltration. This is the first record of a capillarid nematode from the kidney of the mongoose.


Quantitative determination of serum immunoglobulins in chronic obstructive pulmonary diseases with special reference to cor pulmonale. In patients with chronic obstructive pulmonary disease, quantitative analysis of serum IgG, IgA and IgM were carried out by use of the radial immunodiffusion method. The concentration of IgE in sera was also determined by the radioactive radial immunodiffusion method. The mean value of serum IgG level in the group of cor pulmonale was higher than that in groups of emphysema, asthma and normal subjects. The mean value of serum IgA level was higher in two groups of cor pulmonale and emphysema than in the group of asthma and of normal subjects. There was no difference of serum IgM levels between these four groups. The mean value of serum IgE in the group of allergic asthma was higher than that in the group of non-allergic asthma or normal subjects and an elevated serum IgE level was also found in patients with cor pulmonale. Findings of this study suggested that the raised level of serum IgG in patients with chronic cor pulmonale might reflect production of antiheart antibodies against cardiac tissue.


Renin release and the juxtaglomerular apparatus in chronic renal failure due to glomerulonephritis. Changes in renin release with decreasing renal function and juxtaglomerular apparatus in chronic renal failure due to glomerulonephritis were studied. Renin production was still remaining in the contracted kidneys and its release could be stimulated by various stimulations, although the macula densa didn't seem to play an important role in renin release in the contracted kidneys.


Possible existence of prorenin in rabbit renal cortex. The possible existence of prorenin (inactive form of renin) in rabbit renal cortex was studied. Renal cortex was homogenized with 0.25 M sucrose. Following centrifugation at 800 g for 10 min, the supernatant (Fraction H) was centrifuged at 20,000 g for 20 min. The pellet (Fraction G) was separated from soluble fraction (Fraction S). Intravenous injection of non-treated Fraction G caused a little blood pressure elevation in rats. However, the pressor activity of the same amount of Fraction G which was frozen and thawed 5 times previously was more than 10 times as that of non-treated one. The renin activity in preincubated fraction G with Fraction S was 2-fold the summation of the activity of renin in each fraction, when measured using indirect method. Fraction S lost not only the renin activity but also the renin activating activity when heated for 5 min at 100 degrees C. Exposure to pH 3.5 made no influence on renin activity in either Fraction G and S.


Plasma renin activity in acute respiratory acidosis. Plasma renin activity in acute respiratory acidosis and the effect of hexamethonium bromide was studied. Fourteen mongrel dogs were anesthetized with sodium pentobarbiturate and given 5% and 15% carbon dioxide in room air, successively. Hexamethonium bromide was given to 8 dogs prior to carbon dioxide inhalation. Arterial carbon dioxide partial pressure, oxygen partial pressure and pH were measured in addition to the determination of plasma renin activity. Plasma renin activity was elevated in moderate respiratory acidosis induced by 5% carbon dioxide inhalation from 37.5 +/- 8.8 ng/ml to 52.8 +/- 7.0 ng/ml. In severe respiratory acidosis produced by 15% carbon dioxide inhalation, plasma renin activity elevated further to 85.8 +/- 8.6 ng/ml. Plasma renin activity of the hexamethonium bromide treated dogs was 19.0 +/- 3.5 ng/ml during room air breathing. The activity was elevated to 26.0 +/- 6.4 ng/ml by 5% carbon dioxide inhalation and further to 57.3 +/- 5.9 ng/ml by 15% carbon dioxide inhalation. These findings may suggest that the elevation of plasma renin activity in acute respiratory acidosis induced by carbon dioxide inhalation is independent from sympathetic stimulation.


Newly constructed spatial circumference electrocardiograph. Spatial circumference electrocardiograph which could compute the length of the spatial circumference of each vectorcardiographic loop and record the pattern of the increment of the vectorcardiographic are very easily and accurately on the bed side was constructed. CR differentiating circuits with three sets of the different time constant were used for the differentiations. Vector sum circuits applying absolute value operations reported by Imai and Nguyen Phuoc (1972) were used in this apparatus and the simplification of the consitution of the circuits and the excellent results were obtained. Examples of the spatial circumference ECG recorded in a normal subject and some pathologic conditions were demonstrated.


Immunological reactions involving leukocytes: II. Studies of a patient with multiple transfusion-induced allogeneic antibodies to leukocytes. A patient who had received multiple transfusions for the treatment of aplastic anemia was studied. There were antibodies against allogeneic granulocytes and lymphocytes in her serum. Granulocyte antibodies were detected by their effect on phagocytosis-associated hexose monophosphate shunt (HMS) activity, while lymphocyte antibodies were detected by their cytotoxicity. Granulocyte antibodies were in the IgG and IgM fractions. Prior absorption of this patient's serum with granulocytes or lymphocytes from a single donor eliminated its inhibitory effect on phagocytosis-associated HMS activity of that donor's granulocytes. Studies of many unrelated donors and the patient's seven siblings revealed that her serum inhibited the granulocytes from all of these individuals with the sole exception of the granulocytes harvested from her one HL-A identical sister. The evidence suggests that this patient's antibodies are directed against HL-A antigens or some cell surface antigens coded for by loci closely linked to the HL-A genes.


Continuous trans-physeal traction: a simple method of bone lengthening. Leg length increments utilizing continuous traction across the epiphyseal plate of growing puppies produces a cleavage fracture at the level of the primary metaphyseal trabeculae which neither anatomically nor physiologically alters the growth plate. These fractures heal readily and growth resumes following removal of the traction device. This method is relatively atraumatic to the extremity. It does not require an open operation and the controlled distraction rate has not caused neurological or vascular complications. The study was designed to determine the anatomic site of fracture; no attempt was made to achieve maximal leg length increments.


The use of intramuscular propantheline in the short bowel syndrome. A patient is presented with the short bowel syndrome who because of massive diarrhea was unable to maintain fluid and electrolyte balance with the aid of the usual antidiarrhea drugs. Her bowel remnant consisted of approximately 155 cm of proximal jejunum. Intramuscular propantheline has allowed this patient to function normally for the past 36 months. Balance studies are presented to document the benefit of this drug. Propantheline's effectiveness is probably secondary to its marked slowing of intestinal motility.


[Central core disease with "structured cores" in type II-fibers (author's transl)]. Report on a sporadic case with congenital slowly progressive neuromuscular disease. Light microscopic, ultrastructural and histochemical changes of muscle biopsy reveal the characteristics of central core disease with "structured cores" in type II-fibers.


The morphological and clinical course of the different forms of glomerulonephritis. From repeat biopsies of 291 patients with glomerulonephritis, the clinical and morphological course of the individual forms of glomerulonephritis were compared with one another. From these results we concluded that: 1. Every form of glomerulonephritis has in all probability its individual course and prognosis, which can only be very slightly influenced by therapy. 2. The prognosis is considerably worsened, when either hypertension develops or tubular lesions in the morphological sense of an acute renal failure.


[Diurnal variation in the urinary excretion of vanilmandelic acid and aldosterone in normal and hypertensive persons (author's transl)]. The excretion of creatinine, VMA, and aldosterone was measured in the urine of 14 normal and 25 persons with stable essential hypertension during a day time (7.00-23.00 hrs) and a night time (23.00-7.00 hrs) period. Excretion of VMA and aldosterone per hour in the two groups was lower during the night period than during the day. No significant difference existed between the excretion values of both groups. Night/day rations for the excretion of VMA and of aldosterone respectively, per mg of creatinine excreted were calculated for each subject. In the normal group the night/day ratios of aldosterone excretion showed a significant correlation to the corresponding ratios of VMA excretion; such correlation was not found in the hypertensive group. The fact that a correlation was observed in the normal group is compatible with the hypothesis that catecholamines are involved in the regulation of the renin-angiotensin-aldosterone system. Lack of correlation in the group of hypertensive does not as yet allow any conclusions. In two patients with primary hyperaldosteronism, the aldosterone excretion during the night was relatively more elevated than in the day.


[Aggressive emperipolesis in chronic hepatitis (author's transl)]. The penetration and destruction of hepatocytes by lymphocytes (aggressive emperipolesis) has been described and examined quantitatively in biopsy specimens of chronic hepatitis. In 48 cases of chronic persistent hepatitis, the aggressive emperipolesis was rare and, obviously, not of great importance: sometimes there was no emperipolesis at all. In 36 cases of chronic aggressive hepatitis, however, emperioplesis was always evident, usually even to a very high extent.


Red cell aggregation in blood flow. I. New methods of quantification. The rheological behavior of normal and pathological red cell aggregates in viscometric flow (artificial flow in cone plate chamber) is studied by direct microscopy, (rheoscopy) viscometry and photometry. Marked differences between normal and pathological blood are measured in the microrheological properties of red cell aggregates; only discreet differences are measured by blood viscometry (macrorheology). Both in normal and abnormal blood, red cell aggregation is a reversible process in the presence of adequate shear forces; their respective influences on apparent blood viscosity at low rates of shear are complex functions of shear rate, shear time, hematocrit and plasma viscosities. Pathological red cell aggregation (RCA) forms more rapidly and extensively than normal RCA. The pathological aggregates frequently have a tendency to grow at low rates of shear and they are highly shear resistant.


Red cell aggregation in blood flow. II. Effect on apparent viscosity of blood. The apparent viscosity of blood strongly increases at low shear in rotational viscometers, this phenomenon is based on the reversible formation of red cell aggregates. The magnitude of this increase strongly depends on the hematocrit value, on plasma viscosity and lastly on the microrheological properties of the aggregates. The independent measurement of the microrheological behavior and the effects on viscosity allows a detailed analysis of the hemodynamic effects of red cell aggregates under defined flow conditions in vivo. The comparative analysis shows that the conventional viscometry strongly underestimates the rheological differences between normal and pathologically intensified aggregation. Based on detailed analysis under defined flow conditions in vitro, the biological significance of viscometric results and the hemodynamic relevance of red cell aggregates are discussed.


Erythrocyte sedimentation rate and C-reactive protein. C-reactive protein (CRP) was isolated from pleura exudate of patients with metastasing cancer and added to plasma of healthy blood donors. Only the addition of excessive amounts of CRP (220 mg/ml) caused a notable increase in erythrocyte sedimentation rate. An immunoelectrophoretic method for the simultaneous quantitative determination of CRP together with C 3 or other acute phase reactants is described.


Somatostatin: a potent inhibitor of ACTH-hypersecretion in adrenal insufficiency. Somatostatin (250 mug as a bolus i.v. and 250 mug as a 1-hr infusion) was administered to 5 patients with adrenal insufficiency of different origin. In each patient a sustained, progressive fall in plasma ACTH was observed during the infusion period. The mean maximal reduction in plasma ACTH was 43.8 +/- 5.9%. After cessation of the somatostatin infusion there was a rise of plasma ACTH to starting levels within 1/2 hour. These findings suggest that somatostatin is a potent inhibitor of ACTH secretion, however, only in a condition in which glucocorticoids are lacking.


[Renal function during passive orthostasis (author's transl)]. Glomerular filtration rate (GRF) as measured by inulin clearance and renal plasma flow (RPF) as measured by PAH clearance were determined in 9 mountaineers before and during suspension. The two step permanent infusion technique was employed for 3 periods of 8 min. GRF decreased from 123.0 +/- 14.9 ml/min to 25.3 +/- 3.9 ml/min and RPF from 791.0 +/- 101.0 ml/min to 129.0 +/- 20.4 ml/min within 24 min. These results are consistent with the occurrence of acute renal failure in mountain climbers after free suspension on the rope even after long periods.


Myelopoietic cell proliferation in granulocytopenia of refractory anaemia and preleukemia states with hyper-plastic bone marrow. Myelopoiesis in healthy subjects and in 8 cases of refractory anaemia or preleukemia states was studied by the combined cytophotometric-autoradirgraphic method. Granulocytopenia was present in 7 of 8 cases. The results can be summarized as follows: 1. Mature myelocytes of healthy persons are a mainly differentiating cell population with a low labelling index (3-10%) and a high incidence in G1-Phase. 2. The proliferating immature granulopoietic cells of the patients with neutropenia showed a reduced labelling index with 3H-thymidine as a result of an arrest of these cells in G1 (6 of 8 cases). 3. A similar result was obtained in the study of the hyperplastic myelopoiesis  of an other patient with preleukemia and normal granulocyte count in peripheral blood, with the disturbance of cell proliferation preceding the symptom neutropenia. 4. Finally in only one case an increased frequency of myelopoietic cells in G2-phase was found suggesting an arrest of cell proliferation in the premitotic phase.


[Free and template-bound RNA polymerase in normal and leukaemic human lymphocytes (author's transl)]. Specific activities are determined of two functional fractions of alpha-amanitin sensitive DNA-dependent RNA polymerases in nucleic from human normal and chronic lymphocytic leukemia lymphocytes. Specific activity of "free" RNA polymerase in CLL corresponds ot 0.133 pmoles (3H)-UMP/10(6) cells as compared to 0.209 in normals. Activities of the "engaged" enzymes are 0.139 in CLL and 0.132 in normals. "Free" enzymes in NL and CLL are completely inhibited by 400 ng/ml Rifamycin AF/013, while the "engaged" enzymes exhibit 70% of their original activity. 1.0 ng/ml alpha-amanitin suppress 50% of the activity of the "free" enzyme in CLL. The "free" enzyme in NL and the "engaged" enzymes in NL and CLL do not show any residual activity in the presence of 1.0 ng/ml alpha-amanitin.


[Laxative abuse, plasma potassium levels and chronic interstitial nephritis due to phenacetin abuse (author's transl)]. In 154 patients with phenacetin nephropathy and in 26 patients with phenacetin abuse but without nephropathy a laxative abuse was observed in 3.2% and 15.4%, hypokalemia (3.5 maequ/l or less) in 9.1% and 3.9, respectively. The mean plasma potassium concentration was normal in both groups (4.45 and 4.22 maequ/l, respectively). These data do not support the recently proposed hypothesis that the simultaneous abuse of laxatives contributes to the development of analgesic nephropathy, and that the absence of laxative intake might explain the lack of renal damage in some phenacetin abusers. Although it cannot be excluded that long-standing potassium deficiency in consequence of an abuse of laxatives or diuretics exerts an additional nephrotoxic effect in some cases, this mechanism seems not to be involved in the majority of patients with phenacetin nephropathy.


Breeding and fertility of the Egyptian spiny mouse, Acomys cahirinus: effect of different environments. 2 years experience with Acomys in one laboratory confirmed the slow breeding rate found by other workers. On moving the animals to another laboratory there was a 2-5 fold increase in breeding rate. The most likely explanation of this change was the presence of copper in the drinking water at the 1st laboratory, due to the use of copper water pipes. The data relating to maintenance and breeding of acomys for 2 years at the 2nd laboratory were analyzed to provide a more representative picture of the species.


A survey of laboratory animal disease in Kenya. Diseases and infections diagnosed in laboratory mice, rats, guinea-pigs, golden hamsters and rabbits at the Veterinary Research Laboratory, Kabete, Kenya, are listed and discussed. Zoonoses encountered included salmonellosis and lymphocytic choriomeningitis. A number of traditionally recognised conditions were recorded but there were some notable omissions. The changing picture in laboratory animal science in East Africa is outlined and attention is drawn to the need for work on related diseases and infections.


Value of combining the erythrocyte sedimentation rate test with tuberculin testing in the control of tuberculosis in baboons. 2 batches of baboon infected with tuberculosis were subjected to serial tests with human and bovine tuberculin, while erythrocyte sedimentation rates were estimated concurrently. In the very early stages most but not all reacted to human tuberculin while fewer responded to bovine material. After further development of the disease, tuberculin tests remained positive while sedimentation rates were raised by 10-30 mm per hour. By the time early spread had occurred response to tuberculin was absent but sedimentation rates tended to increase. Advanced cases always tuberculin negative but sedimentation rates were in excess of 50 mm per hour. Such animals were always in good physical condition and represented an insidious danger to other animals and staff in contact with them. Clinical examination failed to reveal cases of tuberculosis except in the terminal stages and no cases were diagnosed by radiography. 2 animals died from apparent anaphylaxis following inoculation of both types of tuberculin. Results showed that use of one or other of these tests alone would not have made possible the elimination of infection.


Problems associated with the identification of bordetella bronchiseptica. Bordetella bronchiseptica, isolated from rodent nasopharygeal swabs, failed to produce characteristic colonies after 24 hours incubation at 37 degrees C. 4-7 days incubation at 37 degrees C was required to achieve positive motility test results, when isolates later identified as B. bronchiseptica were tested by Craigie tube and soft agar stab methods. The biochemical tests used to identify suspected B. bronchiseptica are specified.


Kibbutz and city children: a comparative study of syntactic and articulatory abilities. This study investigated whether there were any significant differences between the syntactic and articulatory development of kibbutz and city children in Israel. Ninety children between the ages of three and four and a half years were administered a Hebrew adaptation of the Northwestern Syntax Screening Test and a Hebrew articulation test. Receptive and expressive language ability at this age were found to be superior among kibbutz children. There was no significant difference in the overall number of articulation errors made: however, there was a significantly higher rate of sibilant disorders among the kibbutz children.


A comparison of impairments in verbal comprehension, speech, reading, and writing in adult aphasics. A clinical population of 44 aphasics was rated on the severity of impairment of functional communication in each of four language modalities: verbal comprehension, speech, reading, and writing. Comparisons are made of the severity of impairment in each pair of language modalities for the subjects as a group and for individual subjects. Correlations between severity ratings in each pair of language modalities are also presented. The results indicate that all modalities are impaired to approximately the same degree and that differences between severity ratings across all modalities are small for the large majority of aphasic subjects. Writing is usually the most severely impaired modality. A discussion is presented reviewing the important influences that methodology has on the data obtained and on the inferences which may be drawn about the nature of aphasia as a uni- or multimodality impairment.


Pure tone-spondee threshold relationships in functional hearing loss: a hypothesis. A hypothesis is offered to account for the fact that in functional hearing loss, spondee threshold is frequently significantly lower than the pure-tone average. The hypothesis has three basic components: (1) that patients with functional hearing loss use a loudness criterion in making response decisions to suprathreshold stimuli; (2) that, at suprathreshold levels, pure tones and spondee words appear equally loud at equal sound pressure levels, and (3) that calibration values employed in pure-tone and speech audiometry contribute to the aberrant speech-pure tone relationship. Factors that may confound the speech-pure tone relationship are discussed as are the clinical and research implication of the hypothesis.


Distinctive features in speech pathology: phonology or phonemics? Distinctive feature is not a unique concept within linguistic theory. It has two distinct bases: phonemic theory and generative theory. Phonemic theory assumes a direct correspondence between distinctive features (the elements of phonemes) and the speech signal. Although this assumption can be shown to be incorrect, it seems to be the one most widely held in speech science. Generative theory, on the other hand, assumes no such direct relation and consequently can account for certain linguistic phenomena that phonemic theory cannot. This theory then seems to be preferable to phonemic theory for a featural analysis of misarticulation. However, there is a problem. Chomsky and Halle's system (generative theory) as it stands does not deal with the link between what it conceives to be the lowest level of linguistic structure (the phonetic matrix) and speech production. Therefore, Chomsky and Halle's distinctive features cannot be applied fruitfully to all instances of misarticulation. The discrepancy that exists between phonological structure and the speech signal must be accounted for in a theory of speech production. This can be accomplished by recognizing a production matrix below the phonetic matrix, where segments are described in terms of production features. The crucial point is that no one-to-one relationship necessarily exists between distinctive features and production features.


Some common problems in the design and conduct of experiments in stuttering. This paper focuses upon and discusses some common problems in the design and conduct of various types of stuttering research. Apparent problems are dealt with under the following headings: (1) circular definitions of hypothetical constructs, (2) operational definitions of stuttering that lack denotative specificity, (3) research designs based on invalid assumptions about stuttering, and (4) omitted or inadequate measurement of speech rate in operant studies of stuttering. In each of these sections, a problem is identified and then some suggestions are offered for resolving it. This article pertains to research that underlies clinical management, and some implications for clinicians are drawn.


Response-contingent small-step treatment: performance-based communication intervention. Response-contingent small-step treatment is a structured clinical methodology developed for use in the management of brain-injured communication-disturbed adults. Treatment planning includes the determination of target behavior hierarchies, criterion levels of patient performance, and planned response contingencies. Treatment forms are employed to enable ease in scoring patient response, systematic treatment planning, and objective data retrieval for charting.


Verbal versus tangible reward for children who stutter. The results of several previous attempts to modify the speech of stuttering children suggest that tangible (as opposed to verbal) rewards are efficient forms of positive reinforcement. The purpose of this study was to investigate the relative effectiveness of these two forms of positive reinforcement with young stuttering subjects. Each of three stuttering children was seen for a base rate session, three experimental sessions, and a carry-over session. Experimental sessions were preceded by verbal instructions explaining the response contingency, and subjects selected their own verbal and tangible reinforcers. Results revealed that both verbal and tangible reinforcements were equally effective in modifying the speech of the three subjects. The possibility is discussed that the effectiveness of verbal and tangible rewards with young stuttering subjects can be explained by (1) selection of the rewards by the subjects; (2) reinforcement of easily identifiable behavior; and (3) subject awareness of the response contingency.


The effects of age on the diagnostic utility of the rollover phenomenon. Articulation curves were constructed for 32 elderly listeners with hearing according to previously established decadal age norms. Each function was specifically examined for the presence of rollover, a previously determined indicator of retrocochlear pathology. Rollover exhibited by six subjects was an age-related phenomenon. It is suggested that normal neural degeneration concomitant with the aging process is responsible for this auditory behavior and that clinical significance of the rollover phenomenon is greatly reduced when dealing with the aged population. Implications for the use of the rollover index are also discussed.


Paroxysmal ataxic dysarthria.

Paroxysmal ataxic dysarthria. This report reviews 13 cases in which a dysarthria appeared, remitted, and reappeared within seconds. The speech pattern of each case was characteristic of ataxic dysarthria. A cinefluorographic film for one of the subjects provided a rare opportunity to study the articulatory dynamics of this disorder. Multiple sclerosis either was given as a diagnosis or was strongly suspected in each case, and carbamazepine has been an effective treatment. Speculations concerning the origin of the paroxysmal and ataxic character of the dysarthria are presented along with a preliminary checklist for identifying the disorder.


Investigations of the possible role of proteases in altering surface proteins of virally transformed hamster fibroblasts. Virally transformed fibroblasts have on their surfaces zero or reduced amounts of a large external transformation-sensitive (LETS) glycoprotein. This protein is extremely sensitive to proteolysis. When prelabeled normal fibroblasts are cocultivated with transformed cells, the LETS glycoprotein of the normal cells shows an increased rate of turnover. Experiments are described which investigate the possibility that this phenomenon and the absence of LETS glycoprotein are due to proteolysis by the transformed cells. In particular, the role of plasminogen activation is examined by the use of protease inhibitors and plasminogen-depleted serum. It is concluded that activation of plasminogen is not required for the disappearance of the LETS glycoprotein although the involvement of other proteases cannot be ruled out. The role of proteases in affecting cell growth and behavior is discussed.


Selective modification of cell surface proteins and thymidine transport in hamster cells exposed to cholera toxin. The increased adherence and morphological response which occurs in Chinese hamster ovary cells as a result of exposure to cholera toxin is paralleled by modification in the relative exposure of outer proteins. Mild proteolysis treatment of the cells prelabeled with [3H] glucosamine reveals a markedly different kinetics of release of external glycopeptides as a result of exposure to cholera toxin. Selective alterations in external tyrosyl-rich proteins can also be detected by lactoperoxidase-catalyzed radioiodination. The above modifications are accompanied by a decrease in the rate of thymidine uptake by toxin-treated cells.


Cell surface receptors and their dynamics on toxin-treated malignant cells. The binding, mobility, and mode of cell entry of the plant toxin ricin (or RCAII) were investigated on susceptible and partially resistant murine cell lines. When susceptible cells (SV40-transformed 3T3 fibroblast cells and BW5147 lymphoma cells) were examined, ricin bound rapidly, induced endocytosis, and entered the cell cytoplasm via broken endocytotic vesicles to inhibit cell protein synthesis, as found previously (1). Addition of lactose within 15 min after initial ricin binding prevented toxicity. After this time lactose addition no longer blocked the inhibition of protein synthesis. In a partially resistant lymphoma (BW5147/RCA3) that shows only a slight reduction in the total number of ricin-binding sites, ricin bound rapidly to the cell surface, but was endocytosed significantly less at low ricin doses compared to its parental line, indicating a possible difference in cell surface behavior. The exposed surface proteins on the BW5147 parental and BW5147/RCA3 resistnat lines were examined by 125I-labeling utilizing lactoperoxidase-catalyzed iodination. The radiolabeled components were solubilized and separated by slab electrophoresis in sodium dodecyl sulfate. Autoradiograms of the slab gels indicated that two surface components of approximately 80,000 and 35,000 mol wt were much less exposed or were missing on the resistant line.