Effects of density of noise field and delay on information-processing of patterned inputs. The present paper extends current research on pattern recognition to investigate the effects of density of noise field and delay (off) times. The results indicate major effects due to density of the noise field, on time and off time. Recognition initially declined as the density of the interference increased, then subsequently leveled off at higher interference levels. The effect of density of noise field on recognition was not consistent across all levels of either on time or off time. There was a wide range of processing-ability among Ss. Some subjects were capable of high levels of performance for very fast input rates. There was a high degree of consistency among Ss in the relative difficulty of the letters.


Inability to wink an eye and eye dominance. Data from 56 subjects in Grades 4 to 12 only able to wink one eye indicated that the non-winking eye was used as the dominant one in tests on monocular and binocular sighting.


Interhemispheric EEG activity in normal and aphasic adults. EEG recordings were obtained from 10 normal and 10 aphasic adults while they participated in behavioral tasks and a no performance condition. Interhemispheric asymmetry and average activity at 5 to 15 Hz in each hemisphere were compared between groups and among tasks. Asymmetry during behavioral tasks did not differ significantly between groups or among tasks, however, aphasic patients showed a significant decrease in average activity at 5 to 15 Hz on the behavioral tasks compared with the level for a non performance condition. Test-retest reliability was poor for asymmetry but acceptable for average activity at 5 to 15 Hz. Finally, asymmetry was significantly related with severity of aphasia.


Knowledge of results and counting of sequential light flashes by retarded and intellectually normal children. 12 adolescent retarded boys and 12 younger third-grade boys of equivalent mental age observed sequential flashes of light and made judgements of numerosity. Durations of successive flashes were 300, 500, 700, and 900 msec. in trains of 1 to 15 flashes. Over six daily sessions, subgroups receiving information about accuracy after each trial made fewer errors, and subgroups receiving none made more errors. The linear trend interaction of knowledge of results with IQ was significant, with performance of the nonretarded boys decreasing to the level of the retarded boys in the no-feedback condition. With feedback, the rate of decrease in error over sessions was similiar for both levels of IQ. The results indicate a substantial effect of knowledge of results in improving accuracy of counting by retarded and nonretarded children, this effect being relatively greater for the latter group.


Unilateral control of bilaterally symmetrical movement as factor in lateralization of speech. A number of bilaterally symmetrical motor patterns, preformed in their basic topography at brainstem levels, are most efficiently guided through unilateral input. This concept is related to the lateralization of speech.


Temporal summation effects on lingual vibrotactile thresholds. Ascending lingual vibrotactile thresholds at 250 Hz were obtained for four groups of normal-speaking adult subjects. Stimulus durations were varied for each respective group. Threshold values plotted as a function of stimulus duration indicated a temporal summation function occurring up to 200 msec. Findings are discussed in terms of underlying peripheral receptors and some implications for using summation measures as a basis for identifying oral sensory disorders.


Lateral eye-shift related to preschoolers use of descriptive language. Lateral eye-shift in preschool children was related to the use of more nouns in description by 14 right-shifters, more adjectives by 19 left-shifters. Results suggest that differences in hemispheric activity may lead to differential language use.


Primacy effect in orienting responses to auditory stimuli of tones and music. This 25 factorial experiment investigated the primacy effect in the orienting response. The type of stimuli (tone or "music"), stimulus intensities (loud or soft), length of adaptation period (same, 5 or 30 sec; or different, 5 min.), interstimulus intervals (5 or 30 sec.), and sex were studied. College students, 32 males and 32 females were randomly assigned to each group. In the same condition, the tone (or music) was soft (or loud) for 5 sec. (or 30 sec.) in adaptation and was then changed alternately without interruption to loud, soft, etc. (or soft, loud, etc.) for 5 sec. (or 30 sec.). The different condition was identical except for the length of adaptation period in which the stimuli sounded continously for 5 min. Analyses of the GSR manifestation of the orienting responses indicated: (a) an over-all primacy effect with the auditory stimuli and (b) the primacy effect occurred in the 5-sec.-same but not in the 30-sec.-same condition as predicted.


IES Arrow-Dot longitudinal comparisons of personality development in preschool children. 19 preschool children were administered the IES Arrow-Dot test at the beginning and end of the school year and results compared with those based on a similar sample gathered the previous year. Trends for declining Impulsivity and Superego development were corroborated. Baselines for Impulsivity and Superego showed variation. Baselines and trends of rising Ego scores were supported. Results suggested suport for developmental trends of personality integration in preschool children.


Exercise-produced arousal, film-induced arousal and attribution of internal state. Respiration rates were monitored on 20 high-anxious and 20 low-anxious female Ss while they were watching an anxiety-provoking film on industrial accidents. Half of the Ss exercised on a bicycle while half remained seated throughout their viewing of the film. Exercised Ss reported feeling less anxious while viewing the accidents compared with the inactive controls. All groups recalled similiar amounts of the film content, and respiration rates did not increase while viewing the accidents. Results of self-report of anxiety are consistent with a recently advanced theory of objective self-awareness.


Internal-external locus of control and performance on a vigilance task. Previous studies have demonstrated there are large individual differences in subjects' ability to perform on vigilance or watch-keeping tasks. This study used Rotter's Internal-External (I-E) Locus of Control Scale to resolve some of the variance attributed to individual differences and tested the hypothesis that the internally controlled person is a better monitor on a vigilance task than an externally controlled person. There were 64 subjects who performed a visual watch-keeping task for a 1-hr. period. The results indicated that the external subjects made significantly more incorrect responses and missed significantly more signals during the entire task. The internal-external dimension was effective in differentiating performance on a vigilance task.


Eye movements in response to real and apparent motions of acoustic targets. Monitoring of eye movements resulting from the tracking of sound displacements in total darkness confirmed the generally accepted idea that smooth pursuit cannot be induced in the absence of a real visible target. Exclusively saccadic movements were obtained with real and apparent displacements of a constant frequency source and with frequency variations associated to spatially calibrated positions through training for 5 Ss. Smooth pursuit eye movements were only observed if S was allowed to point and follow with his hand the perceived position of acoustic targets.


Cultural deprivation and the relative preferences of preschool children for complex and novel stimuli. To determine their relative effects, the preferences of 19 deprived and 21 non-deprived preschoolers for both complex stimuli and novel stimuli was measured. The deprived children showed a lack of preference for the novel stimuli but did not differ from the non-deprived group in their preference for complexity. These data clarified a confounding of these stimulus dimensions which had occurred in previous studies.


Preferred hand and steadiness of reaction time. RT and its left/right difference of both biceps muscles were measured by electromyogram (EMG) in the bilateral simultaneous flexion of elbows using 11 left- and 13 right-handed subjects under four conditions which were combinations of two factors; warning signal; presence or absence of a fore-period, and position of limbs; elbow 90 degrees or 135 degrees. EMG-RT and its left/right difference were influenced by these factors. The effects of warning and position of limbs were not the same on the preferred and non-preferred hands. It was assumed that EMG-RTs of biceps in the preferred hand were less influenced by these experimental conditions.


Evidence from mutual masking for serial processing of tachistoscopic letter patterns. This experiment deomonstrates serial processing of tachistoscopic patterns when all potential artifacts are eliminated. Two nonsense arrays of six letters were tachistoscopically exposed successively at the same positions. Ss identified more letters from the temporally first string on the left of fixation and more from the second on the right, indicating left-to-right serial processing. Variations among Ss in the location of the crossover in curves indicate individual differences in processing time. Therefore, this study demonstrates definitively that tachistoscopic performance at various positions can reflect a sequential left-to-right processing of information, possibly at different rates.


Studies on age specific leukocyte and lymphocyte counts in normal Friesian, Red Danish and Jersey cattle in Denmark. To determine reference values for leukocyte and lymphocyte counts of the most common cattle breeds in Denmark, especially regarding the evaluation procedure in the leukosis control work, blood samples from normal cattle of all ages within the Friesian, the Red Danish and the Jersey breeds were collected, transported and counted as in the routine leukosis control programme. Evaluation of breed- and age specific counts showed numerically small, but statistically significant differences in mean values of leukocytes and lymphocytes between on the one hand Jersey cattle and on the other hand Red Danish and Friesian cattle. However, it was found that the key values used in the leukosis control work are generally in good agreement with the calculated 99.87 percentiles of all age- and breed specific lymphocyte count distributions, which were approximated with logarithmic normal distribution curves.


The effect of storage temperature on the second day methylene-blue test. The purpose was to show whether or not the methylene-blue test can be postponed to the second day. The milk samples were preserved at three different temperatures 8-10 degrees C, 5-7 degrees C and 2-4 degrees C and the storage time was 16-22 hours. The test indicated that the methylene-blue test could be postponed to the next day satisfying the practical needs if the samples were kept at temperature of 2-4 degrees C. At this temperature the results of the second day methylene-blue test differed statistically not more than p less than 0.05 (nearly significant) from the results of the first day methylene-blue test and the percentage of quality class changes was 2.9.


[Angioneurotic hereditary edema. 3 faimilial cases]. The study involved three families with hane. This disorder differs from the simple condition by its preferential visceral localisation (digestive and, above all, the glottis), its hereditary nature and the existence of a biological abnormality affecting the serum complement system (absence of enzyme activity of C1 esterase inhibitor in the serum of sufferers). The cases reported illustrate the serious prognosis of this condition (four deaths by asphyxia in one family) in the absence of specific therapy. This disorder, although rare, represents a fundamental problem in general pathology by virtue of the enzymatic, immunological and genetic processes which it involves.


[Correlation between immunological stage and response to chemotherapy. Results in 64 patients with nonresectable epidermoid bronchial cancers]. Out of 64 patients with non resectable epidermal bronchus carcinoma, 26 patients had before any treatment positive responses of skin tests to recall antigens. In these patients we found a correlation between the reactivity of the skin tests, the presence of seric factor inhibiting the leucocyte migration, the mean count of peripheral lymphocytes and the response to combination chemotherapy protocol. The patients who respond to treatment have a significantly better prognosis than the patients who do not respond. With this correlation it seems to be possible to distinguish between two populations of patients with non resectable bronchus carcinoma : one population with a good risk who can be significantly improved by the treatment : the other population with a very severe prognosis.


[Arterial embolization in the treatment of metrorrhagia of tumoral origin]. The authors report two cases of embolisation of the genital arteries in patients suffering from emtrorrhagia due to a tumour and in whom surgical treatment was impossible. The results of embolisation were satisfactory.


[Substitutive treatment of hemopjolia A using a new factor VIII concentrate]. Thirty children with classical hemophilia were treated with an intermediate-purity factor VIII concentrate prepared according to Newman. The average factor VIII concentration was 21 U/ml. Thirty five injections ranging from 3 to 55 U/kg of body weight were given. The clinical efficacy was satisfactory, no side effects were observed. In vivo factor VIII recovery was initially very high, decreased rapidly until the 4th hour post infusion and then slowed according to a biological half-life of 11.3 +/- 3.1 hours. A satisfactory correlation between the dose injected and the factor VIII in vivo recovery was observed only after four hours post injection. The commercially available concentrate contains in average 25 U of factor VIII per ml, 13 U/mg of fibrinogen and 2.03 U/mg of proteins. It can be injected with a seringe and is therefore adapted to home care treatment. In addition, it is convenient for prolonged treatment with high doses of factor VIII as required by surgical procedure of treatment of patients with an inhibitor, avoiding limitations caused by hypervolemia or hyperfibrinogenemia.


Function of DNA polymerase I in RNA-primed synthesis of bacteriophage M-13 duplex DNA. Cell-free extracts from Escherichia coli contain a DNA polymerase activity resistant to SH-blocking agents, which is capable of synthesizing complementary strand DNA on a circular M-13 DNA template by extension of RNA primers. This activity is considered to be identical with DNA polymerase I (or some altered form of this enzyme) since it is missing in extracts from po1A- cells. DNA synthesis in the presence of SH-blocking agents occurs at a reduced rate as compared to untreated controls and leads to the formation of DNA chains of defined size (0.4-0.5 genome's length). It is concluded that efficient M-13 duplex DNA synthesis requires the cooperation of both DNA polymerase I and III.


New phosphoramidates as protecting groups in ribooligonucleotides synthesis. N, N-Dimethyl-p-phenylenediamine, glycine amide and p-methylthioaniline were condensed with uridine 5'-phosphate and the phosphoramidates obtained were tested for their stability in anhydrous pyridine, 50% aqueous pyridine or 80% acetic acid. The p-methylthioanilidate (IIc) was oxidized to give p-methylsulfoxylanilidate of uridine 5'-phosphate (IId) which was found to be 5 times more stable than the p-methylthio compound. The p-methylsulfoxylanilidate of 2'-O-benzoyluridine 3'-phosphate was condensed with the mononucleotide to yield the dinucleotide, MMTrU(OBz)-p-U(OBz)-p in 28% yield.


The effective synthesis of uridylyl/3'-5'/5-methylcytidylyl/3'-5'/guanosine. The triester method was adapted to the synthesis of uridylyl/3'-5'/5-methylcytidylyl/3'-5'/guanosine. As the protecting groups 4-methoxy-5,6-dihydro-2H-pyran for 2'-OH and 5'-OH groups of uridine and 2'-OH group of 5-methylcytidine, methoxymethylidene for I:3'-cis-diol system of guanosine, and benzoyl for the amino groups of 5-methylcytidine and guanosine were used. The obtained product was characterised by UV, electrophoresis, chromatography, an enzymatic digestion and alkaline hydrolysis.


Analysis of the possible helical structures of nucleic acids and polynucleotides. Application of (n-h) plots. The two helical parameters n and h where n is the number of nucleotide residues per turn and h is the height per nucleotide residue have been evaluated for single stranded helical polynucleotide chains comprising C(3') -endo and C(2') endo class of nucleotides. The helical parameters are found to be especially sensitive to the C(4')-C(3') (sugar pucker) and the C(4')-C(5') torsions. The (n-h) plots display only one important helix forming domain for each class of nucleotides characterized by the sugar pucker and the C(4')-C(5') torsion. A correlation between the (n-h) plots and the known RNA (A,A') and DNA (A,B,C) helical forms has been established. It is found that all forms of helices except the C-DNA possess a favorable combination of P-O torsions. The analysis of the (n-h) plots suggests that C-DNA can have a conformation very similar to B-DNA. Although the (n-h) plots predict the stereochemical possibility of both right-handed and left-handed helices, nucleic acids apparently prefer right-handed conformation because of the energetics associated with the sugar-phosphate backbone and the base.


The methylation of adenovirus-specific nuclear and cytoplasmic RNA. Each poly(A) containing cytoplasmic AD-2 MRNA contains at its 5' terminus the general structure m7 GpppN1 pN2p or m7 GpppN1mpN2mpNp as well as an average of 4 m6A and 0.5-1 m5C residues per molecule. Almost all of the N1m residues are adenine derivatives including Am, m6Am  and probably m26,6Am. The N2m is mostly Cm but small amounts of the other three methylated bases are also present. All the methylated constitutents of mRNA are distant from the 3' terminal poly(A). The amount of m6A appears to be greater in larger mRNA than in smaller mRNA. Nuclear Ad-2 specific RNA also contains caps, m6A, and m5C with about twice as much m6A relative to caps as cytoplasmic mRNA. The similarity of Ad-2 nuclear and mRNA to HeLa hnRNA and mRNA suggests that adenovirus mRNA production is a good model for eukaryotic mRNA production.


Tritium labelling of nucleic acids accompanied by conversion of cytosine to uracil. A new method of incorporation of tritium into nucleic acids with an accompanying conversion of cytosine to uracil is proposed. The method is based on the reaction of nucleic acids with bisulfite in the presence of 3H2O. Under certain conditions poly(C) is quantitatively converted to a radioactive poly(U), whereas similar bisulfite treatment of poly(U) does not result in any tritium incorporation. Specificity of the reaction is confirmed by the results of analysis of modified tRNA and rRNA. Incubation of tRNA with bisulfite and 3H2O does not lead to cleavage of the polynucleotide chain. Similar treatment of the denatured DNA results in tritium incorporation into DNA which is accompanied by a conversion of cytosine to uracil. There is virtually no reaction between native DNA and bisulfite. Only certain cytosone residues in yeast tRNAVal/2a interact with bisulfate providing that reaction is carried out under sufficiently mild conditions.


Base composition heterogeneity of mammalian DNAs in CsCl-netropsin density gradient. DNAs of 15 mammals and some lower organisms were analysed by CsCl-netropsin density gradient centrifugation. Increased resolving power of this method enabled to detect many new components in mammalian DNAs. Distinct components were detected in the density range of the main band. These components found in different mammalian DNAs have probably limited variation in the G+C content. Most of other components seems to be species specific. The DNAs of lower organisms form homogeneous band even in the presence of netropsin. The relation between densities in CsCl-netropsin and CsCl density gradient is nonlinear. This result supports a hypothesis that in high ionic strength netropsin is preferentially bound to (dA.dT) clusters.


The binding of poly(rA) and poly(rU) to denatured DNA. I. Model studies with homopolymers. We have compared the properties of the poly(rA).oligo(dT) complex with those of the poly(rU).oligo(dA)n complex. Three main differences were found. First, poly(rA) and oligo(dT)n do not form a complex in concentrations of CsCl exceeding 2 M because the poly(rA) is insoluble in high salt. If the complex is made in low salt, it is destabilized if the CsCl concentration is raised. Complexes between poly(rU) and oligo(dA)n, on the other hand, can be formed in CsCl concentrations up to 6.6 M. Second, complexes between poly(rA) and oligo(dT)n are more rapidly destabilized with decreasing chain length than complexes between poly(rU) and oligo(dA)n. Third, the density of the complex between poly(rA) and poly(dT) in CsCl is slightly lower than that of poly(dT), whereas the density of the complex between poly(rU) and poly(dA) in CsCl is at least 300 g/cm3 higher than that of poly(dA). These results explain why denatured natural DNAs that bind poly(rU) in a CsCl gradient usually do not bind poly(rA).


The binding of poly (rA) and poly (rU) to denatured DNA. II. Studies with natural DNAs. We have studied the interaction of poly(rA) and poly(rU) with natural DNAs containing (dA.dT)n sequences. The results indicate that hybridization of poly(rA) to denatured DNA can be used to estimate the size and frequency of large (dA.dT)n tracts, whereas hybridization with poly(rU) does not give reliable information on these points. In 6.6 M CsCl, poly(rU) can form stable complexes with denatured DNA containing short (dA)n tracts (n less than or equal to 6), whereas binding of poly(rA) to denatured DNA under these conditions requires much larger (dT)n tracts (estimated n greater than 13). Moreover, binding of poly(rA) requires pre-hybridization in low salt, because free poly(rA) precipitates in 6.6 M CsCl.


3-Ribosyl-6-methyluracil as a phosphate acceptor in the synthesis of the internucleotide bond catalyzed by pancreatic ribonuclease. The synthesis of uridylyl-(3'-5')-3-ribosyl-6-methyluracil (UprmU) catalyzed by pancreatic ribonuclease (EC 3.4.1.22) has been performed using uridine 2', 3'-cyclic phosphate (U greater than p) as phosphate donor and 3-ribosyl-6-methyluracil (rmU) as phosphate acceptor. The rate of synthesis of UprmU is much higher than that of uridylyl-(3'-5')-uridine (UpU) in a control experiment under the same conditions with uridine as acceptor. The yields of UpU and UprmU were 20 and 17% respectively. The competitive hydrolysis of the initial U greater than p also proceeds faster when rmU is used as the acceptor. The relationship between the conformation of this nucleoside and its acceptor activity in the enzymatic synthesis of the internucleotide bond is discussed.


Sepcific fragmentation of DNA heteroduplex molecules of two bacteriophage lambda mutants with endonuclease Si from Aspergillus oryzae. Heteroduplex DNA molecules of two bacteriophage mutants (lambda b2 and lambda i434ct68) were obtained by the method of molecular hybridization. These heteroduplexes possessed two types of loops formed as a result of: a) deletion in one of the DNA strands; and b) substitution of a DNA fragment for nonhomological one. The digestion of heteroduplexes with single-stranded specific nuclease SI from Aspergillus oryzae produced two fragments at 37 degrees C and three ones at 55 degrees C. The separation of fragments and determination of their molecular weight were carried out by means of electrophoresis in agarose. The molecular weights both measured and preliminarily calculated proved to be close. One of the fragments was identificated by its biological activity in CaCl2-dependent infectious system with helperphage.


On the possibility of excimer state formation in homodimers of the pyrimidine bases. A detailed investigation of the ground state and the first excited state of the pyrimidine bases of uracil, cytosine, thymine and their stacked homodimers is carried out by the Extended Hückel method. The first exicted state is shown to be the (see article)-state. The excitation is localized mainly on the C5-C6 bond region. The energy dependence of the ground state and the first excited state of the homodimers on the mutual position of bases is considered. For every dimer a minimum on the exicted state potential curve is found. This fact creates the possibility for the formation of excimer. The obtained data give some reason for the suggestion about participation of the excimer state as a precursor of photodimer.


Interpretation of the properties of chromatin extracts from mammalian nuclei. Chromatin from standard preparations of nuclei stabilized by magnesium ions at 0-4 degrees C was degraded during the nuclear isolation, and newly-synthesized chromatin was degraded slightly more slowly than the ;older' chromatin. The significance of this observation, and its relation to the interpretation of the properties of nucleoprotein extracts is discussed.


2',3'=Carbonates in the synthesis of uridine 5'-deoxy and 2',5'-dideoxy derivatives. Detritylation of 2',3'-O-carbonyl-5'-O-trityluridine (Ia) with ethereal hydrogen chloride affords 2',3'-O-carbonyluridine (Ib; 83%) which is converted by mesylation to the 5'-mesylcarbonate Ic (75%). Reaction of compound, Ic with tetrabutylammonium bromide in DMF affords the 5'-bromo carbonate Id (77%) which is reduced with tributyltin hydride to the 5'-deoxyuridine 2',3'-cyclic carbonate Ie (70%). When heated with imidazole, compound Ie affords the 2,2'-anhydro derivative IIa (76%) which is converted to the 2'-chloro derivative IIIa (88%) on heating with HC1/DMF. The tributyltin hydride reduction of compound IIIa gives 2',5'-dideoxyuridine (IIIb; 68%). When heated with NaHCO3 in DMF, the 5'-bromo carbonate Id affords the anhydro bromo derivative IIb (50%) which is converted to the 2',5'-dichloro derivative IIIc (86%) on heating with HC1/DMF. The tributyltin hydride reduction of compound IIIc affords the 2',5'-dideoxy derivative IIIb (59%). Alkaline hydrolysis of the 2,2'-anhydro derivative IIa affords the arabinosyl derivative IVa which is converted to the diacetyl derivative IVb (34%) by acetylation. When refluxed in water, the 2',3'-cyclic carbonates Ib, Id, and Ie are hydrolysed to the parent nucleosides, namely, uridine (Va; 81%), 5'-bromo-5'-deoxyuridine (Vb; 78%), and 5'-deoxyuridine (Vc; 83%). Hydrolysis of carbonates Ib and Ie is accompanied by the formation of the 2,2'-anhydro derivatives IIc (10%) and IIa (5%) as by-products.


Search for an adenine photoproduct in DNA. Poly(d[14C]A), p(dA)2, and [14C]adenosine-labeled DNA were irradiated at 254 nm with fluences up to 50 J/m2, and then following formic acid hydrolysis at 170 degrees C WERE SUBJECTED TO PAPER CHROMAtography using a butanol:water:acetic acid (80:30:12) solvent system. For poly(dA), up to 25% of the radioactivity appeared as fluorescent material located in the Rf 0.21-0.29 region. The hydrolysate of the purified photoproduct, p(dA)2, isolated from irradiated p(dA)2 by DEAE chromatography also had an Rf of 0.29 as well as an absorbance maximum at 310 nm. In all cases studied, however, the photoproduct yield in the Rf 0.29 region for native DNA was less than 2%. Denaturation of the DNA appeared to enhance the yield slightly, although no pronounced peak in this region of the chromatogram was discerned. Mechanistic studies indicate that the yield of the adenine photoproduct in poly(dA) is favored by base stacking, has a singlet excimer as a precursor, and is quenched by hydrogen bonding to a pyrimidine. It is concluded that the yield of the adenine photoproduct in both native and denatured DNA is considerably less than in poly (dA) and in all probability does not represent a biologically significant product.


Modification of end phosphate gruops in mono- and oligonucleotides. A method is described for selective activation of phosphomonoester end groups of oligonucleotides and nucleosidedi-(tri) phosphates via mixed anhydrides with mesitoic acid. Mixed anhydrides are synthesized in high yield and isolated by paper or DEAE-cellulose column chromatography. The ability of such anhydrides to phosphorylate different nucleophilic agents was used for synthesis of amidates, imidazolidates, esters, thioesters and pyrophosphates of mono- and oligonucleotides. Mixed anhydrides mono-, oligonucleotides and nucleosidedi-(tri)phosphates and mesitoic acid were also applied to achieve immobilization of the mono- and oligonucleotides via their end groups on hexamethylenediamine - Sepharose support. Mixed anhydrides studied may be efficiently used for affinity labeling of proteins and nucleic acids and also as material for preparating reagents for template reactions.


Reaction of diazoalkanes with 1-substituted 2, 4-dioxopyrimidines. Formation of O2, N-3 and O4-alkyl products. In non-aqueous solution, diazomethane and diazoethane react with the O2, O4 and N-3 sites of uridine, thymidine, 1-methyluracil and 1-methylthymine. Diazoethane has a higher affinity for alkylating oxygens than does diazomethane. The relative ratio of O2:O4:N-3 methyl products is 1:2:16 and of ethyl products the ratio is 1:1:2. When the diazoethane reaction is performed in neutral buffered solution, the same proportion of O2:O4:N-3 ethyl products is found, but the extent of reaction is very low. O2-alkylation greatly labilizes the glycosidic bond of thymidine and uridine toward acid hydrolysis. All O2 and O4 alkyl 1-substituted 2,4-dioxopyrimidines are dealkylated in weak acid but the O2 alkyl group is the more stable.


Scintigraphy with 99mTc-tripolyphosphate in the early diagnosis of osteomyelitis. In 14 consecutive patients with suspected osteomyelitis studies were performed with 99mTc-tripolyphosphate. In all patients the diagnosis was ultimately verified by biopsy. In seven of the 14 patients the radiograms showed osseous lesions at the same time as the scintigraphic studies. In the other seven patients, such lesions became visible on the X ray film one to 20 weeks after their demonstration by the radioisotope method. 99mTc-tripolyphosphate appears to be a very sensitive indicator of oesteomyelitis.


[Determination of radio-iodine in struma cysts with regard to radio-iodine kinetics]. After oral administration iodine 131 is demonstrable within hours and days in thyroid cyst fluid. This iodine concentration may be caused by active metabolism in the cyst, depending on thyroid function. Highest levels of iodine 131 are found in autonomous nodules.


Scintiscan characteristics of normal thyroid gland in a region of known iodine intake. Morphological features of a normal thyroid gland in a geographical region where the daily iodine intake is about 1 mg are established. The mean weight of the thyroid gland is 31.3 gm with a range from 19 to 43 gm. Oblique length of the right lobe is 5.0 cm and that of the left lobe 4.8 cm. The surface area of the right and left lobes is 9.7 and 9.1 cm2, respectively. The weight of the thyroid gland calculated on the basis of the scan obtained with 99mTcO4 is quite variable and shows poor correlation (gamma = 0.40) with the weight obtained on the basis of I-131 scan. It is suggested that the criteria of normalcy be established regionally based on iodine intake, and that an isotope of iodine be used in calculating the weight of thyroid gland for dosimetry purposes.


[Studies of hepatobiliary function with a scintillation camera and electronic data processing)]. The authors performed the sequential scintigraphy after intravenous application of 131I-BSP using the scintillation camera. During the first 60 minutes the pictures of the liver and of the mesogastrium were recorded in one-minute intervals on the digital magnetic tape. The following scintigrams were then recorded after 3 and 24 hours after the injection. Choosing the region of interest the authors noted the histograms above the liver and above the mesogastrium, evaluated then the proportion of impulse rate above the liver and mesogastrium in measured intervals and obtained using the digital computer the velocity coefficient of the radioactivity accumulation in the liver as liver chromoexcretive function parameter. The results were compared with the standard test of the 45-minutes retention of the BSP, with the analysis of the curve measured above the temporal region, with the clinical status as well as with the results of other auxiliary examination methods. According to the first experiences gained when examining 91 individuals the described method proves to be very useful.


99mT-c-pyridoxylidene glutamate a radiopharmaceutical for the hepatobiliary system. 99MTc-pyridoxylidene glutamate is a good agent to visualize the hepatobiliary system. The method of preparation and quality control, and preliminary data on its behaviour in human controls is presented. In humans the gall bladder could be well seen in about 15--20 minutes after intravenous injection.


[Heart-blood-pool scintigraphy in dialyzed chronic uremic patients)]. The author have carried out scintigraphic measurements of the heart in order to diagnose the presence of an exudative pericarditis 50 times in 26 dialyzed uremic patients. Within the one year's observation 13 patients had an exudative pericarditis from the beginning on. In 8 cases a regression of the exudation could be observed. The examination has a low risk for the patient and is suitable to demonstrate clinically relevant pericardial liquid accumulation.


Calcium metabolism in children suffering from homozygous beta-thalassaemia after oral administration of 47Ca. The study of calcium metabolism in ten thalassaemic children comperatively with controls after oral administration of 47Ca has shown diminished intestinal absorption. It is suggested that this finding is propably related in part with the pathogenesis of the osteoporosis in thalassaemia.


Quality control and testing of 99mTc-macroaggregated albumin. 99mTc-labelled macroaggregated albumin particles prepared from a commercial "kit" have been tested in detail. With the introduction of the method of gel chromatography column scanning it became possible to make fast and simple quantitative measurements of the radiochemical purity. This was found to be the only reliable method for quantitative determination of non-aggregated 99mTc-labelled albumin. The measuring results of the kit in question have shown a labelling efficiency of about 97--99%. In addition to a few percent of 99m-Tc-pertechnetate less than 0.5% of disturbing radioactivity was found. The labelling was stable for at least 5--6 h. About 80% of the particles are in the size-range of 10--80 mum. A rough estimation of the number of particles in the solution resulted in 0.4-10(6) per mg of MAA. The dynamic studies of the lung uptake and the elimination of 99mTc-MAA in man resulted in a biological half-time in the lungs of about 1--2 h. The radiation absorbed dose to the lungs per mCi administered 99mTc-MAA was estimated to 0.1 rad. Less than 1% of the radioactivity was accumulated in liver, spleen or kidneys during the first 25 minutes after injection.


[Biostability of plastic endotheses].

[Biostability of plastic endotheses]. These is shown in this paper the literature review and author's studies on the in vitro and in vivo ageing changes in plastic endotheses under certain conditions. The changes occurring in endotheses exposed to no major mechanical loads were considered in particular. Polyamides, polyethylene derivatives, polyesters, polyurethanes and silicone rubbers were found to belong to the polymers unstable under biological conditions.olyesters, polyurethanes and silicone rubbers were found to belong to the polymers unstable under biological conditions.


[Spontaneous intrauterine rupture of contraceptive Lippes loop]. Basing of 2 clinical cases, the spontaneous intrauterine rupture of contraceptive Lippes loop has been discussed. The analysis of complications accompanying application of contraceptives of this type is presented.


[Disinfection of medical orthopedic articles made of polymer materials]. In traumatological hospitals the medical orthopedic articles made of polymer materials are contaminated by different microorganisms including pathogenic staphylococci, therefore they can serve as a factor of transmission of hospital-associated staphylococcal infections. Polymer materials used for medical orthopedic articles are not bactericidal. Mechanical cleaning with detergents does not provide sufficient elimination of microorganisms from orthopedic articles. This can be obtained by means of the treatment (immersion, wiping) with solutions of bactericidal preparations of various chemical orogin (chloramine, lithium hypochloride, chlorine, sulphochlorantine, hydrogen peroxide, peracetic acid, nitran). Medical orthopedic articles must be decontaminated before use. In the hospitals, disinfection of orthopedic articles should be performed at least once a day. Immersion of the articles made of porous materials into solutions of disinfectans with surface-active additives or into solutions of surface-active bactericides is a simple, readily available and effective method of treatment. Drawback of this method is that disinfectant solution partly remains in the pores and can be eliminated only by means of drying of the article in thermostate. Orthopedic articles of polyurethane foam can be decontaminated only by use of solutions of hydrogen peroxide and nirtan out of all the tested disinfectants. Medical orthopedic articles made of polymer materials have endured repeated treatment with disinfectant solutions without any change of their physical and mechanical properties. The usability of these articles did not deteriorate.


[Ethylene oxide sterilization and disinfection of plastic articles]. The outline of progress in the use of ethylene oxide sterilization of thermolabile plastic articles is presented. The explosiveness, adsorption and toxicity of the ethylene oxide and the influence of humidity on its bactericidal effectivity are especially emphasized. The methods of checking-up sterilization processes are indicated.


[Morphology and histochemistry of tissues surrounding liquid and cured silicone rubber]. The process of encapsulation of SKTN liquid silicone rubber of 30-50 thousand molecular weight, and of SKTN compounds curing in the tissues was observed experimentally. The silicone implants were surrounded by mature connective tissue after 3.0-4.5 years. The method of silicone administration, whether by injection or by implantation of prefabricated solid prostheses, exerted no effect on the local tissue reaction.


A comparative study of talampicillin and ampicillin in general practice. Talampicillin hydrochloride, the phthalidyl ester of ampicillin, when given  in a dosage containing the equivalent of 125 mg of ampicillin four times daily for six days to general practice patients, has proved to be as effective, both clinically and bacteriologically, as 250 mg of ampicillin given four times daily for six days. Although the over-all incidence of side-effects was similar with both preparations, diarrhoea was significantly reduced in talampicillin-treated patients. Possible reasons for this are discussed. Talampicillin therefore offers certain advantages over ampicillin in producing effectiveness equivalent to that of ampicillin at a lower dosage level with a reduced incidence of diarrhoea.


Rapid purification of a high molecular weight human brain protein by chromatography on controlled pore glass. Controlled pore glass (CPG) chromatography was employed to simply (one pass through column) and rapidly (60 minutes) purify a human brain specific protein having a high molecular weight (approximately 250,000 mol. wt.) from a crude brain extract containing proteins of varying molecular weights. This method, either exactly as described herein or by adjusting the pore size of the CPG, should be adaptable to other purification problems.


A single column packing for the gas-liquid chromatographic separation of various biologically important compounds. The use of a single, commercially available column packing, TabsorbR, is described for the g.l.c. separation of a large number of different compounds. The resolution of the homologous members of the following series of compounds was achieved: (1) saturated fatty acids (C1-C18), (2) normal aliphatic saturated dicarboxylic acids (C2-C14), (3) normal aliphatic saturated alcohols (C1-C24), (4) normal aliphatic saturated amines (C1-C12), (5) the common amino acids except arginine, histidine and cysteine, (6) aliphatic hydrocarbons (C10-C20) and (7) monosaccharides. It should be noted that twenty-two monosaccharides including three hexosamines and two anhydrohexoses, could be resolved as alditol acetates in a single run. In addition, galacturonic, glucuronic and iduronic acids could be separated from one another as their 1,4-lactones. The resolution achieved in these series of compounds was found to be consistent and highly reproducible. It is of further interest that certain isomers of the higher fatty acids and hydrocarbons with one double bond could also be separated from the normal and saturated compounds, respectively. The applicability of "Tabsorb" for the g.l.c. separation, although noted above to be considerably broad, is by far not yet exhausted. These procedures which form the basis for the quantitative determinations of the various compounds studied as demonstrated by analysis of glycopeptides for neutral hexoses and proteins for the amino acids, can readily be adapted to preparative methods. From the biochemical point of view "Tabsorb" is an extremely versatile column packing in that it can be used for the identification of many of the common building blocks of natural products.


[Some aspects of evaluating the thyroid gland scanning image normally and in pathology]. The authors studied 1252 scannograms of 1170 patients. Plane image variants of the shape of normal, dystopic and maldeveloped thyroid gland were studied. Scannographic image of the structure of pathology of the organ and peculiarities of absorption and distribution of radioactive indicators (I131, Tc99m), depending on the histological picture of the pathological process and other factors stimulating or blocking its function are discussed. Practical significance of the method in clinical endocrinology is emphasized and sources of diagnostic errors possible in interpretation of the form and structure of the scannographic picture of the gland under normal and pathological conditions are elucidated.


[Comparison of the nature of insulin secretion, determined by radioimmune and biological methods, normally and in diabetes mellitus]. The character of insulin secretion curves was studied under normal conditions and in diabetic patients during glucose tolerance test. Patients with diabetes mellitus were divided into the following groups: donors, patients with reduced glucose tolerance and with manifest diabetes. Insulin was determined in paralleled by 3 methods (biological ones and radioimmune one). An increase in immunoreactive insulin was seen in the group of patients with reduced glucose tolerance during the whole course of the test with a peak at the 30th minute and a simultaneous reduction and a sharp fall at the 60th minute of free insulin level. Apparently biologically less active forms of insulin and proinsulin are secreted which possess immunological properties and react well with antibodies to insulin.


[Experience with the tegretol treatment of diabetes insipidus]. The use of tegretol in a 24-hour dose of 200-600 mg in 12 patients with diabetes incipidus led to the improvement of their general condition, lesser thirst, reduction of the 24-hour diuresis and increase of specific gravity of the urine. A positive effect of prescribing tegretol together with chlorpropamide was noted in a patient with diabetes mellitus and incipidus.


[Sex hormone reception in the human endometrium in normal and pathological pregnancy]. A study was made of reception of 17 beta-estradiol, progesterone, and testosterone in human endometrium during pregnancy. In the cytozol of the endometrium each of the sex steroids was bound by three different receptor components, two of which were represented by highly specific proteins. The maximal capacity of specific cytozol receptors to each of the sex steroids was measured in normal and pathological pregnancy; in the latter case the number of receptors to progesterone proved to be decreased. It is supposed that one of the specific binding components is necessary only under conditions of increased functional load upon the organ.


[State of the cardiovascular system in chromophobe adenoma of the hypophysis]. The detected changes in the cardiovascular activity, primarily in the state of hemodynamics in patients with chromophobic adenoma of the hypophysis indicated a possible phasic character of the course of this disease. A sharp fall in the contractile capacity of the myocardium in a number of patients was most probably caused by marked hormonal and metabolic shifts developing against the background of insufficiency of the adeno-hypophysis. On the basis of the data obtained a detailed study of the state of the organs of circulation is recommended in all the patients with chromophobic adenoma of the hypophysis, particularly during the preoperative preparation.


[State of the bone marrow hematopoiesis and peripheral blood in obesity]. Peripheral blood was studied before and after the treatment in 186 patients with various forms of adiposity; bone marrow was examined in 10 patients. The data obtained were compared with the STH level in the blood serum in the corresponding patients. The bone marrow of patients with adiposity displayed a reduction of myelokariocytes on account of mature neutrophils and normoblasts, a fall in reticulocyte and megakariocyte count and an increase in lymphocytes. Peripheral blood is characterized by reticulocytopenia, a reduction of leukocytes on account of granulocytes and an accelerated ESR. Possibly the detected shifts in myelogram and the peripheral blood were caused by the principal pathological process and the change of the somatotropic function of the adenohypophysis.


[Role of different regions of the hypothalamus in regulating the hypophyseal-adrenal cortex system in rats]. The effect of electrical stimulation of various structures of the medial hypothalamus on the corticosterone level in the peripheral blood was studied in rats with preliminarily destroyed individual areas of the hypothalamus. Injury of various structures of the medial hypothalamus led the first days after the operation to reduction of the response to the stimulation. Two-three weeks after destruction of the anterior and the middle portions of the hypothalamus reactivity of the hypophyseo-adrenocortical system was restored, although not always completely. Only in destruction of the premamillary nuclei there was no restoration of the reaction. A conclusion was drawn that the area of the posterior hypothalamus was of great necessity for functional regulation of the hypophysis-adrenal cortex system.


[Interrelationship of the pineal body with the hypothalamo-hypophyseal complex. II. The effect of melatonin on the incorporation of H3-leucine in the anterior and medial hypothalamic nuclei of pinealectomized blinded rats]. A study was made of the effect of low doses of melatonin on the H3-leucine incorporation into the supraoptic, paraventricular, ventro-medial, and arcuate nuclei of the hypothalamus. Sexually mature blinded pinealectomized female rats were used in these experiments. Melatonin administered in a dose of 10 mug at 11 a.m. for 9 days inhibited the biosynthetic potential of the mentioned hypothalamic nuclei enhanced by extirpation of the epiphysis. Administration of melatonin at 11 p.m. in the same doses produced no effect on leucine incorporation.


[Congenital disorders of the thyroid gland in experimental litters obtained from mothers subjected to immunization and the action of I-131]. I131 injected to rabbits immunized with homologous thyroid gland on the 19th-20th day of pregnancy led to congenital disturbances of the thyroid gland in the experimental litters in the I and II generations. The functional activity and the activity of proteolytic enzymes of the thyroid gland decreased by the 30th day of the postnatal development. There was revealed a distinct correlation between the content in the thyroid gland of the experimental litters of DIT + MIT and of organic forms--T3 + T4. During the first days after birth DIT + MIT content showed a sharp fall and increased by the 30th day; on the contrary, iodine binding into organic forms decreased with the progress of age. Histological examination of the thyroid glands in the experimental litters demonstrated lymphoid-plasmocytic infiltration, along with connective tissue proliferation, which led to a marked sclerosis and fibrosis of tissues, hypofunction. Beginning from the age of one month there was seen an extensive nodular proliferation, which possibly served as the beginning of parenchymatous goiter formation. The appearance of lymphoid-plasmocytic infiltration in the thyroid gland of the II generation is considered to be the result of congenital predisposition to the autoimmune thyroiditis inlaid in the memory cells and intensified during birth.


[Electrolyte metabolism in hypothyroidism]. Sodium, potassium, calcium, magnesium content was determined in the blood plasma, erythrocytes and the 24-hour urine. There was revealed an increased sodium content in erythrocytes, a fall of plasma potassium level, a reduction of urinary potassium excretion and of potassium-magnesium coefficient of the urine; a tendency to increase in the calcium and magnesium level was seen in erythrocytes. Only calcium content in erythrocytes became normal after the treatment.


[Reaction of the animal adrenal cortex to the action of a strong, constant magnetic field and to a hypomagnetic environment]. Experiments were conducted on male albino rats; a study was made of the functional condition of the adrenal cortex following a single 30-minute (decapitation of the animals in 1, 8, 16, and 24 hours, and in 4, 8, 16, and 30 days) and repeated (for 30 minutes every day) prolonged (for 1 month) action of CMF (decapitation in 2, 4, 8, 16, and 30 days) and hypomagnetic field (for 8 and 30 days). Changes in the lipid content and in the alkaline phosphatase activity served as a morpho-histochemical criterion. A single brief CMF action proved to stimulate the adrenal function the first hours of the after-action; later the changes were oscillating in character. Prolonged repeated action of CMF depressed the functional activity of the adrenal cortex. Both in the first and in the second cases functional activity of the organism was almost completely restored by the end of the month, after the cessation of the CMF action. An 8-day stay of the animals in the hypomagnetic medium led to activation of the adrenal gland function, and a 30-day stay--to its reduction.


[Change in aldosterone secretion by the adrenals and in its urinary excretion in rats under the action of acid polysaccharides]. Repeated administration of chondroitin sulfate to rats in doses of 20 and 120 mg/kg/24 hours led to reduction in the level of urinary aldosterone excretion in rats. Carboxymethylcellulose and alginic acid displayed no inhibitory effect. Urinary aldosterone excretion decreased in rats 24 hours after a single administration of chondroitin sulfate; this effect persisted for the following 3-4 days. The maximal reduction of aldosterone excretion (by 50%) occurred on the 3rd and the 4th day after administration of the preparation. Intraperitoneal injection of chondroitin sulfate to rats diminished the rate of aldosterone secretion by the adrenal glands; as to corticosterone secretion--it remained unchanged.


[Blood proteins, thiol group metabolism and testosterone content in prostatic dysfunction]. A study was made of the blood serum proteins, thiol groups, aldolase activity, testosterone content in rabbits under conditions of insufficiency of the prostate gland caused by its suturing. It was revealed that at the late postoperative periods there was an increase in the blood serum proteins of albumin fraction, and a reduction of the thiol group level (SH-free, masked and disulfide). Aldolase activity increased at the early periods and then sharply fell. The incretory function of the testes was disturbed.


Physical therapy program for the pediatric cardiac surgical patient. The management of pediatric cardiac surgery patients at the North Carolina Memorial Hospital is described. The physical therapy program includes education and treatment which is intended to 1) reduce surgical risk by decreasing preoperative pulmonary congestion, 2) reduce the incidence of postoperative pulmonary complications and length of hospitalization, and 3) improve patient and family understanding and cooperation. The program includes a self-instructional package presented by a children's puppet. The package prepares the patient for the events ahead, including the physical therapy program and prospectively frightening aspects of intensive care.


Physical therapy management of patients through cross modality. Cross modality is a method for channeling therapeutic services from various health disciplines (modalities) through a single therapist who provides direct treatment and care. A pilot project at the University of Michigan's Institute for the Study of Mental Retardation and Related Disabilities tested this method in three mental retardation institutions. The ward attendant was designated as the primary therapist. The project staff included a physical therapist, speech therapist, occupational therapist, and nutritionist who taught ward attendants selected evaluation and treatment procedures. In theory, cross modality broadens the role of the institutional physical therapist to include teaching, monitoring of physical therapy services, and consulting with an institutional cross modality team which includes therapists (physical, occupational, speech), a dietitian, nurse(s), supervisor(s), and ward attendants. In practice, the therapist's attitude toward cross modality and the receptiveness of the ward attendant determine, the success of cross modality in any setting.


Perception of coarticulated lip rounding. This study investigates the perceivability of coarticulated lip rounding in French. Nine utterances containing the clusters /kstr/, /rstr/, and /rskr/ followed by one of the vowels /i/, /y/, or /u/ in all possible combinations, were truncated at 4 different points before the vowel. Test items in each of the 4 groups therefore contained different amounts of information regarding the nature of the following vowel, due to coarticulatory influences of the vowel on the preceding consonants. Subjects were asked to predict the identity of the missing vowel. Results show that when segments up to and including at least half of the final consonant of the cluster are present, subjects correctly identify the missing vowel well above chance level. Several individuals were able to identify the vowel even when presented with shorter versions of the utterances. No significant difference in performance was found between French and English subjects, nor between subjects with and without phonetic training. Pereceivability of individual features of the missing vowel is discussed. It is concluded that coarticulatory effects due to lip rounding (as well as to horizontal place of articulation) provide perceivable information at a level significantly above chance, and that this information may be used by the perceptual mechanism as an aid in speech sound identification.


An alternative operation for inverted nipple. An operation to correct inverted nipples is described. Its main advantage is that reinversion of the nipple is prevented, because two "dermal" flaps not only increase the density of tissue underneath the nipple but also act as slings.


A method for reconstruction of the central slip of the extensor tendon of a finger. Congenital absence of the proximal interphalangeal joint extensor mechanism was corrected by using the lateral band and attached intrinsics of an adjacent finger. This technique has worked well in 3 cases.


Elastogenesis in healing wounds in bats. Full-thickness square wounds were made in the webs of fruit bats. Elastin was laid down in the healing scars of these wounds in two forms--as single fibers under the regenerated epidermis, and as bundles of fine fibers taking their origin and orientation from the cut ends of the web elastin bundles. This process continued over a period of 6 months, during which time other components of the scar became reduced in amount. The arrangement of the new elastin in the scar tissue was thus a replica of that seen in the normal web. There are indications in this study that elastin imparts elasticity to the scar.


Cephalometric study of facial growth in children after combined pushback and pharyngeal flap operations. Linear and angular cephalometric measurements of children who had had combined palatal pushbacks and superiorly-based pharyngeal flaps do not show later growth retardation of the face. There was an inherent tendency for children with overt clefts of the secondary palate, classic submucous clefts, or occult submucous clefts to demonstrate pre-operatively a narrow SNA and SNB--but the difference between these angles (ANB) was normal.


Mammaplasty with curved incisions.

Mammaplasty with curved incisions. We present a modification of Pitanguy's technique of mammaplasty. Curved incisions are used and the breast tissue is resected on a horizontal plane. We have done this operation in 130 consecutive mammaplasties and have had no complications. The esthetic results have been good.


"De-epithelization" of the dermal pedicle in a reduction mammaplasty. Eleven biopsies in 6 consecutive patients having reduction mammaplasties by a technique which uses a dermal pedicle have established that dermis does remain in the pedicle. The term "de-epithelization" is not correct and should be abandoned in this context.


Skin testing for prognosis or therapy formulation in cancer patients: caveat emptor. Delayed cutaneous hypersensitivity to DNCB was assessed in 56 patients who had epidermoid intraoral carcinoma, prior to therapy. They were followed for 44 months, and their skin reactivity correlated well with both a small tumor size and a favorable clinical prognosis. In a significant number of cases, however, lack of reactivity did not indicate a fatal outcome. Therefore, we recommend caution in the use of any skin test data in formulating therapy. When cured cancer patients were skin-tested, there was no correlation between the state of the disease and their skin reactivity.


A new mastopexy operation for mild or moderate breast ptosis. A new procedure for mastopexy, with or wtihout an augmentatiom mammaplasty, is presented. Its advantage is the minimal resultant scarring in the least conspicuous location.


Frontonasal tumors: their diagnosis and management. Fifty-three patients with frontonasal tumors were seen in a 17 -year period. Of these patients, 33 were females (62 percent) and 50 were caucasians (94 percent). The correct diagnosis was made preoperatively in 87 percent of the cases. Eighty-five percent of the patients were children, and most of the lesions (83 percent) were developmental anomalies rather than true neoplasms. The most common lesions were dermoids (17), hemangiomas (16), and encephaloceles (9), but 13 different types of lesions were encountered. The treatment of all is surgical excision, and a cooperative effort by the neurosurgeon and the plastic surgeon is required in some cases.


Correction of asymmetries of the breasts. The problem of asymmetries of the breasts is discussed. The technical details of one operation for unilateral reduction are described.


Skin tension lines in the domestic pig.

Skin tension lines in the domestic pig. The skin tension lines of the domestic pig have been mapped. With a 6mm punch biopsy, local relative skin forces can be determined. The influence of various factors  (such as age, fat deposition, gascial attachments, and previous scarring) on the direction of the skin tension lines is discussed.


Hemangiopericytoma of the hand. Case report. We present one case of hemangiopericytoma of the hand. This lesion's clinical resemblance to glomus tumor is stressed; only the histological report could confirm the diagnosis.


Giant breast implant for unilateral aplasia. Case report. The authors present a patient who had aplasia of the right breast associated with absence of the pectoralis muscles and a chest wall deformity. The insertion of combined breast-chest wall implants improved her appearance markedly.


Psychoanalytic approaches to artistic creativity. Study of data and findings from clinical and applied psychoanalysis suggests a number of common factors encountered with uncommon frequency and intensity in creativity. In this paper, early childhood experiences, sensorial perceptions, body feelings, loneliness, reactions to loss, and body imagery of several artists are discussed. Problems relating to body image in creativeness are explored.


Transference regression and real experience in the psychoanalytic process. At the patient's level of reality testing and integration in the transference neurosis, the analyst serves as an effective parent who halpd the 'child' master various danger situations. This results in the patients relearning and development as the analyst provides new experiences for the regressive childhood components of the patient's personality. There is also a sequential or simultaneous cognitive element which is important in the ultimate development of mastery and is particularly useful in the process of working through. This cognitive element, however, cannot be effectively used until there have been repeated new and real experiences for the fixated and/or regressive components of the patient's personality.


The influence of the person of the analyst on structural change: a case report. This paper attempts to demonstrate how values and attitudes of the analyst are inevitably communicated through the interpretative process and how they lead to structural change. Details of the analysis of a woman with a defective feminine self-representation are presented. A transference fantasy of the analyst's wife led to a fantasied ideal object with subsequent identification and modification of structure.


The future of psychoanalysis and its institutes. If the function of psychoanalytic training institutes is to train practitioners of psychoanalytic technique, it is unreasonable to expect institutes to offer effective training in research or other scholarly applications of psychoanalysis. But if one insists that the training of psychoanalysts should, at least in some instances, encompass more than the teaching of psychoanalytic therapy, the training institutes have been failing in their function to educate candidates for scholarly activities. Whether the structure of the independent training institutes can permit such broadened training is questioned. One solution is to move the educational function into the university setting.


The psychoanalytic treatment center as a transference object. Three Treatment Center cases are presented. They reveal resistances and technical difficulties having to do with a split in the transference-the analyst viewed as the depriving mother and the Institute viewed as the nurturant grandmother. Possible implications for the conduct of student analyses are described.


Effects of alcohol on male sexual responding. Sixteen adult males participated in a repeated measures design in which they served as their own control to determine the effects of various amounts of alcohol on: (1) their sexual arousal elicited by erotic motion-pictures, as measured by a penile transducer, (2) the ability to voluntarily inhibit their arousal to those same films in accordance with instructions, and (3) the ability to become voluntarily aroused in the absence of overt stimuli. The ingestion of a low (0.5 or 0.6 ml/kg) or a moderate (1.0 or 1.2 ml/kg) amount of alcohol resulted in a small, but significant, depression of mean sexual arousal, but other measures were not affected. However, the ingestion of a high (1.5 or 1.8 ml/kg) amount of alcohol resulted in every measure of evoked arousal being depressed by a comparatively large degree. The high level of alcohol also affected a very large decrease in sexual arousal when subjects were instructed to become sexually aroused in the absence of overt erotic stimuli. In contrast, none of the three amounts of alcohol caused a significant impairment in the ability of subjects to voluntarily inhibit their sexual arousal, even though most subjects experienced some deterioration in that ability after ingesting a moderate amount of alcohol. The actions of alcohol on sexual responses were not significantly correlated with its effects on a nonsexual matching task, were not related to subjective reports of how alcohol usually affects sexual behavior, and were generally not related to reported drinking history.


Selective neuroendocrine effects of low-dose haloperidol in normal adult men. The neuroendocrine effects of haloperidol, usually reported as side effects of this drug when given in antipsychotic doses, have not been systematically investigated. In the present study five normal adult men were administered saline and two doses of of haloperidol (0.25 mg, 0.5 mg) intramuscularly in a double-blind randomized block design. The anterior pituitary hormones GH, LH, FSH, and PRL were measured in blood samples taken every 20 min for several hours thereafter. The low doses of haloperidol used have been shown by others to alter the human EEG; in our subjects these doses produced no objective or subjective clinical effects. There were no drug related changes in GH, LH, or FSH. PRL, however, showed a prompt, statistically significant, dose-related increase in plasma levels, with a return to baseline with 5 h. Haloperidol has strong dopamine-blocking effects, and the hypothalamic inhibitory mechanism for PRL release is believed to be dopamine-mediated. The results of this study suggest that haloperidol may have utility in low doses primarily for its hypothalamic neuroendocrine effects, and that dose-related PRL release may be a useful paradigm for comparing dopamine-blocking antipsychotic agents in humans.


Sensory, perceptual, motor and cognitive functioning and subjective reports following oral administration of delta9-tetrahydrocannabinol. Three dose levels, 0.2, 0.4, and 0.6 mg/kg, of delta9-tetrahydrocannabinol (THC) and a placebo were orally administered to 10 frequent and 10 occasional marijuana users. Following ingestion of each dose and the placebo, objective tests selected from a battery of sensory and perceptual motor tests routinely used to evaluate cerebral dysfunction in hospitalized patients were administered. The influence of delta9-THC on proficiency and variability of performance was minimal. However, when individual test scores and variabilities were combined and converted to standard scores, allowing for analysis of overall performance, THC had a small but consistent detrimental effect on both proficiency and variability of performance. In contrast, THC exerted profound effects on the subjective experiences of the volunteers as assessed by the Subjective Drug Effects Questionnaire. Subjective changes in mood, feeling, perception and somatic sensations were reported by all subjects but were more pronounced in the occasional user group. It was proposed that the small impairment noted in objective test performance after ingestion of delta9-THC as contrasted to the large effects on subjective responses suggests that the principal effects of marijuana are on the autonomic nervous system rather than on higher cortical functions.


The effects of pre-exposure to scopolamine on subsequent drug state discrimination. This experiment was done to test the prediction that preexposure to a drug state would increase the discriminability of that drug state when used as a cue in a discrimination task. Eight rats were pre-exposed to scopolamine for 20 days and 8 rats were given saline injections. The drug pre-exposed animals acquired a drug state discrimination more quickly than the controls. A difference was also found between the generalization gradients for each group with the pre-exposed group showing a steeper generalization gradient.


Ethanol and isopropanol effects on schedule-controlled responding. The effects of ethanol and isopropanol were studied on responding by pigeons under multiple fixed-ratio (FR), fixed-interval (FI) schedules of food presentation and under a fixed-interval (FI) schedule of food presentation where responding was decreased by punishment. The ethanol was rapidly absorbed into blood and decreased responding within 15 min after intubation to the opening of the proventriculus. Dose-effect determinations of the effects of ethanol showed that ethanol decreased responding in both the FR and FI components of the multiple schedules at similar doses, but there were increases in responding under an FR 100 schedule at lower doses. Isopropanol tended to decrease FR responding at doses that either increased FI responding or did not affect FI responding. Both ethanol and isopropanol (1 g/kg) produced effects on the local rates of responding within the FI which were rate-dependent in that they increased low rates while not affecting or actually decreasing the high rates of responding. Both ethanol and isopropanol increased punished responding if it was not severely suppressed by the punishment procedures.


Effects of caffeine consumption on nicotine consumption. Coffee-drinking cigarette smokers take in more nicotine when they ingest almost no caffeine than when they ingest an amount of caffeine ranging from 75 mg to 300 mg. They do not take in relatively less nicotine as the dose of caffeine increases from 75 mg to 300 mg. It seems, then, that something due to caffeine deficit is responsible for the effect. Heavier users of caffeine show this effect less strongly than do lighter users of caffeine. These results are discussed in terms of the discriminability of caffeine and nicotine deficits and the possible influence of differential tolerance to caffeine. The importance of evaluating caffeine consumption when studying nicotine use and the importance of considering the chronic level and use of these drugs when studying their effects on behavior is indicated.


Therapeutical efficacy of a combination of apomorphine with sulpiride or metoclopramide in Parkinsonism. In healthy volunteers the emetic effect of apomorphine (5-10 mg, i.m.) was prevented by haloperidol (2 mg), metoclopramide (10 mg) and sulpiride (100 mg), injected intramuscularly. In parkinsonian patients, apomorphine (1 mg) given alone ameliorated the neurological symptoms (30% improvement in the disability score), but the improvement was accompanied by nausea, vomiting, sedation or sleepiness. Haloperidol (2 mg) prevented not only the emetic effect of apomorphine (10 mg), but also its therapeutic efficacy in parkinsonism. Indeed, the disability score was worsened by the drug combination in some patients. Moreover, after haloperidol, apomorphine produced deep sedation and sleep. By contrast, in parkinsonian patients pretreated with metoclopramide (10 mg) or sulpiride (100 mg), apomorphine (10 mg) markedly diminished tremor and rigidity and failed to produce nausea, vomiting and sleepiness.


Critical flicker frequency (CFF) and psychotropic drugs in normal human subjects-a review. This literature review presents summary methodological and statistical data on 33 studies in which critical flicker frequency (CFF) thresholds were used to evaluate the effects of acute oral doses of single psychotropic drugs in normal human subjects. In all, 96 drug-dose-study combinations are represented. CFF was found to be altered to a statistically significant degree (P is less than 0.05) in 51 (65%) of the 79 instances in which inferential statistical methods were used to evaluate the results. As expected, stimulants increased CFF while hypnotics decreased it. There is also a discussion of important methodological considerations in the design of psychopharmacological studies employing CFF. While many studies have shown CFF to be sensitive to the effects of psychotropic drugs, there have not always been adequate controls for extraneous factors (especially, set and suggestion, changes in pupillary diameter, and the presence of other commonly used drugs). Finally, consideration is given to the attempts to increase the sensitivity of the CFF test to drug effects.


Effect of doxepin on uptake and efflux of serotonin human blood patelets in vitro. It is shown that the tricyclic antidepressant drug doxepin (comprising 15% of the cis- and 85% of the trans-isomer) is a moderately potent competitive inhibitor of serotonin uptake in human blood platelets in vitro, with an inhibitory constant Ki of about 2 X 10(-7) M. The inhibitory effect was 6 times stronger in an artificial, protein-free medium than in diluted plasma, corresponding to about 85% protein binding. The efflux of serotonin from platelets preloaded with 14C-serotonin was not affected by doxepin in concentrations up to 10(-6) M, but increased rapidly at concentrations above 10(-4) M.


The interaction between prostaglandin E1 and delta 9-tetrahydrocannabinol on intestinal motility and on the abdominal constriction response in the mouse. The interaction between delta9-tetrahydrocannabinol (THC) and PGE1 was studied using two pharmacological parameters-the rate of passage of a charcoal meal through mouse small intestine and the abdominal constriction response in the mouse. PGE1 administered intraperitoneally produced a dose-dependent decrease in intestinal motility, and this effect was antagonized by low (0.25 mg/kg) doses of THC and potentiated by higher doses of THC (1 mg/kg). Kinetic analysis suggested that the interaction was of a mixed but predominantly competitive type. PGF2alpha produced an increase in intestinal motility but this was not dose-dependent. THC antagonized the effect of PGF2alpha in a dose-dependent manner suggestive of a physiological antagonism. THC (0.25-2 mg/kg) antagonized the dose-dependent PGE1 abdominal constriction response in a fashion which suggested a mixed (though mainly competitive) antagonism. It would ssem, therefore, that on the two pharmacological parameters studied THC appears to be interacting with PGE1 at the same receptor site. Although the doses of THC used are within the range of those used in man, it is not implied that these results are necessarily implicated in the psychoactivity of the drug.


Effects of dopamine receptor stimulants on locomotor activity of rats with electrolytic or 6-hydroxydopamine-induced lesions of the nucleus accumbens. Ergometrine (8 mg/kg) injected intraperitoneally into normal rats had little effect on locomotor activity. In contrast, rats with selective 6-hydroxydopamine-induced lesions of dopamine terminals in the nucleus accumbens showed a strong stimulation of locomotor activity following injection of this dose or ergometrine. The dopamine analogue 2-amino-6-7-dihydroxy-1, 2, 3, 4-tetrahydronaphthalene (ADTN) (150 mug), caused strong and long lasting stimulation of locomotor activity when injected intracerebroventricularly into rats. The ADTN response was markedly reduced in rats with bilateral electrolytic lesions of the nucleus accumbens, but unchanged in rats with bilateral electrolytic lesions of the caudate nucleus. At a lower dose level (50 mug) ADTN, injected intracerebroventricularly, had little effect on the locomotor activity of normal or sham-operated rats. This dose of ADTN was, however, effective in causing locomotor stimulation of rats with bilateral 6-hydroxydopamine-induced lesions of the nucleus accumbens. These results support the view that the dopamine receptors in the nucleus accumbens are involved in the actions of locomotor stimulant drugs.


Abolition of the hippocampal theta activity and drug-learning dissociation. Rats were trained to escape electric shock by running into one arm of a T-maze on 10-trial daily sessions with pentobarbital (10 mg/kg), and to the opposite arm of the maze on other sessions with saline, the drug and saline sessions being alternated. This drug-learning dissociation or state-dependent learning was achieved by rats with medial septal lesions, abolishing hippocampal theta activity, as efficiently as rats with cortical lesions and intact rats. The suggested relationship between drug-learning dissociation and the suppression of hippocampal theta waves, produced by the drug, was not evidenced.


Effects of low and moderate doses of chlorpromazine on discrimination learning in the rat. The effects of chlorpromazine on the acquisition of a brightness discrimination with food reward were examined. Doses of 0.5, 1.0 or 2.0 mg/kg of CPZ as well as saline were administered intraperitoneally to 4 groups of 7 Sprague-Dawley rats, 1 h prior to testing. After a 3-week period of habituation and pre-training, rats were tested 20 trials a day, 7 days a week. No drug effect was found on the number of trials to reach a criterion of 18/20 successive correct responses, which required an average of 6.2 days of training. Precriterion latencies, however, showed an increase as a function of increasing dose level. Post-choice latencies were not affected, eliminating motor retardation as an explanation for the latency effect.


Residual effects of hypnotic drugs: evidence for individual differences on vigilance. Twelve healthy volunteers were given butobarbitone 100 and 200 mg, nitrazepam 5 and 10 mg and 2 lactose dummy treatments, at 23.00 hours at weekly intervals over 6 weeks according to a balanced design and using double blind conditions. Performance was studied between 09.00 hours and 17.00 hours the following day. Significant (P is less than 0.05) impairment of tapping rate and digit symbol substitution occurred. No significant differences occurred between performance after active drug and dummy in auditory vigilance, and subjective effects. Examination of individual differences in the response of subjects to the 4 hypnotic drug treatments, compared with their responses after dummy, indicated that subjects could be divided into two groups. One group consistently rated themselved as more alert after hypnotics and their vigilance performance improved. The other group consistently were more drowsy after hypnotics and their performance was impaired. It is suggested that the improvement in the first group resulted from improved sleep quality sufficient to counteract the residual effect of the hypnotic, whereas the second group merely showed the residual effects of the drugs.


Limbic system-adrenal cortex regulation in depression and schizophrenia. Hypothalamo-pituitary-adrenal [HPA] activation and abnormal HPA regulatory mechanisms have been observed in depressed patients. Depressed and schizophrenic patients were studied to determine whether the HPA disturbances in depression are specific to this psychiatric illness or are mediated by nonspecific breakdown of psychological defense mechanisms. Despite the presence of severe ego defense breakdown and considerable secondary depressive symptomatology, the schizophrenic patients had normal HPA function. The depressed patients had elevated urine free cortisol excretion, high CSF cortisol levels, and did not show normal HPA suppression in response to dexamethasone. Within the depressed group significant correlations of HPA parameters were obtained with somatic features but not with ego breakdown features. After recovery depressed patients had more normal HPA function. The results indicate that HPA dysfunction can occur in association with primary depressive illness, that a psychoendocrine distinction can be made between primary depressive illness and secondary depressive symptomatology, and that psychological defense breakdown is not related to these neuroendocrine observations. Attention is drawn to the utility of urinary free cortisol measurement as a valuable index of HPA activation in psychoendocrine studies.


Visceral learning in asthma.

Visceral learning in asthma. The hypothesis of visceral learning has opened a new avenue in the search for a pathway between psychosocial stimuli and physiological changes. To apply this approach to asthma required a technique for the measurement of the airways' patency, which could be interfaced with the strategy of visceral learning. The method of forced oscillations was shown to correlate highly with whole body plethysmography. The rapid output of the instrument was used on line to control a visual reinforcement signal. Forty-six mild asthmatics, blind to the effect sought in order to minimize the role of suggestion, were tested in a series of evolving experiments. In the first series, two groups of subjects (N=15,13) were able, with this sensory feedback, to decrease (p less than 0.01) their total respiratory resistance; subsequently a smaller group of subjects (N=5), who received reinforcement signals unrelated to the state of their airways, showed no such change. Similar results were obtained in the second series of tests; in A-B-B-A order, the subjects (N=13) received either contingent or noncontingent reinforcement; furthermore, the reinforcement was available only if their lung volume was within the range observed during baseline.


Emotional labeling and overeating in obese and normal weight individuals. It was hypothesized that obese individuals respond to unlabeled high arousal by overeating, while no such response was predicted for labeled high arousal states. Obese and normal weight subjects were led to believe that they were hearing their own heart beat, and that it was either fast or slow. A label for this heart rate either was or was not provided, and subjects' eating behavior was measured unobtrusively. The results supported these hypotheses: aroused obese subjects ate more when they could not identify the cause of their arousal than when a label was known. When obese subjects were calm, the presence or absence of a label did not affect their eating. Furthermore, obese subjects showed significant affect reduction following eating. Normal weight subjects were not affected by the presence or absence of an arousal label. Instead, they ate more when they called themselves calm than when anxious, and more when hungry than when full.


Signal detection theory and the psychophysics of pain: an introduction and review. A brief explanation of signal detection theory is presented, followed by a review of the literature in which differences in pain report were analyzed to determine if changes were in sensitivity (physiological processes) or in response bias (a subject's willingness to report that a signal occurred). Three kinds of studies are reviewed: modification, procedural, and comparative and normative. The advantages of using a signal detection approach in pain research are emphasized. It is concluded that experimenters applying signal detection analysis to pain research should give greater consideration to methodological procedures and be cognizant of all possibilities for shifts in sensitivity (d)'.


Intravenous l-DOPA plus carbidopa in depressed patients: average evoked response, learning, and behavioral changes. The peripheral decarboxylase inhibitor carbidopa (L-alphamethyl-dopa-hydrazine) allowed safe intravenous administration of L-DOPA in amounts sufficient to alter cortical average evoked response (AER) and learning function in 13 depressed patients. The apparently rapid conversion of L-DOPA to dopamine, as reported from studies in animals, is consistent with the 20-30 min onset of effects seen in our study. Unipolar and bipolar depressed patients responded differently to the alterations in brain biogenic amines and also to the nonspecific stress of the experiment. Intravenous L-DOPA given acutely had effects on the AER that were similar to those documented with oral dopa given chronically--an augmentation of amplitude--intensity slopes in unipolar patients and a relative reduction of slopes in bipolar patients. In contrast, intravenous L-DOPA did not enhance verbal learning as did chronic oral treatment, but rather was associated with reduced learning compared with placebo infusions. Different neurochemical changes following L-DOPA given in single intravenous doses may be responsible for the different learning and behavioral changes form those found previously with oral dopa administered chronically.


[Radionuclid szintigraphy of spleen alterations (author's transl)]. Selective or systemic imaging of the spleen by visualization of the reticulo-endothelial system together with the liver represents a non-invasive, innocuous examination. This study is indicated in diagnostically unclear diseases of the left upper abdomen with reference to splenic lesions. Spleen scan is able to demonstrate splenic enlargement which is not detectable in 70% of clinically examined patients. Suspicion for rupture or hematoma of the spleen with imminent delayed rupture represents an important up to now neglected indication for scanning. It should be performed prior to angiography. In liver cirrhosis, combination of liver and spleen scan gives important information about splenic size and radiopharmaceutical uptake. Splenic infarcts, tumors, cysts, and abscess may be recognized by spleen scan in different projection.


[Determination of spleen size by roentgenographic and nuclear medicine methods (author's transl)]. Knowing the splenic size is important in many medical diseases. This paper describes methods how to measure the size of the spleen by palpation, scan and a roentgenogram with soft tissue technique. In 486 normals medium splenic length was 12,8 +/- 1,9 cm measured roentgenographically. In 60 patients with splenomegaly the correlation coefficient between roentgenologically determined size and scintigraphically measured splenic length was 0,89, between radiologically determined size and estimated splenic weight by scan was 0.69. Nuclear medicine methods are important for exact evaluation of the size of the spleen, roentgenography is adequate in screening and control examinations.


[False diagnosis of a splenic cyst. A report about arteriography in cystic lesions of the spleen (author's transl)]. A false diagnosis of splenic echinococcal cyst is reported. Angiographic criteria of this misleading lesion are compared with those of three cases with splenic cysts (two were confirmed surgically). Depending on the size of the cyst, spreading and dislocation of branches of splenic arteries as well as definitsmothly outlined parenchymal defects become visible. Surrounding parenchyma may be partially compressed. Calcifications of the cystic wall do not allow differentiation between primary and parasitic cysts. The Casoni test alone is not sufficient as a primary diagnostic tool as it is false positive often. Additional complement binding test for echinococcus is recommended.


[Roentgenologic diagnosis of splenic trauma (author's transl)]. Description of radiologic criteria in splenic trauma. Abdominal plain film: Enlarged shadow of the spleen with blurred margins; elevation - or rupture - of the left diaphragm; homogenous radiodensity in the left hypochondriac region; fractures of ribs or transverse process. Angiography is indicated in doubtful cases to clarify the following lesions: contusion, sub-or extracapsular hematoma, rupture and fragmentation. Possible diagnostic errors in arteriography (63 examined cases) are discussed.


[Delayed rupture of a metastatic-spleen following coeliacography (author's transl)]. A 49-year-old female patient died 7 days after coeliacography due to delayed rupture of the spleen. Apparently banal events are able to cause rupture of the spleen if metastatic or other pathological alterations are present. There is a brief discussion whether or not coeliacography could induce rupture of the spleen.


[Inflammatory renal pseudotumors (author's transl)]. Inflammatory renal pseudotumors represent a not uncommon finding and frequently pose a difficult differential diagnosis. The importance of a thoroughful radiological evaluation with special reference to angiography is stressed.


[Congenital pseudotumors of the kidney (author's transl)]. Congenital pseudotumors of the kidney represent a relatively common urographic finding. Their pathological urographic, and angiographic characteristics are discussed. As a rule a definitive angiographic diagnosis of these benigne lesions is possible and chirurgical exploration is therefore rarly indicated.


[The individual limit of normal spleen size in routine x-ray film (author's transl)]. The evaluation of spleen length from routine x-ray films of the left epigastrium of 97 healthy patients suggested a close correlation to the height of the 12th thoracic and the first three lumbar vertebral bodies. The normal ratio of spleen length to the vertebral height Th 12- L 3 is 0,82 +/- 0,09, two standard deviations are represented by ratios from 0,64 to 1,0. Spleen length over vertebral height Th 12 -L 3 indicates pathologic enlargment of the spleen. 54 patients with diseases which may cause splenomegaly showed spleen lengths mostly over the heights Th 12 - L 3.


[Current status of preoperative localization in primary hyperparathyroidism (author's transl)]. Since Seldinger demonstrated in 1953 parathyroid adenomas by arteriography, numerous localizing procedures have been advocated. Based on our experience with selective venous sampling for measurement of parathyroid hormone by radioimmunoassay, selective arteriography, pneumomediastinography and scanning with 75Se-selenomethionine, a survey on localization techniques used up to now is given and their value and applications are discussed. Preoperative localization of parathyroid tissue should be limited to patients with previous unsuccessful surgery. In these cases we perform selective venous sampling following selective arteriography for demonstration of the venous drainage pattern and subsequent venous catheterization. Pneumomediastinography is recommended in suspected mediastinal parathyroid adenoma. Scanning with 75Se-selenomethionine is not in use because of its limited success.


[Causes for vertebrobasilar artery insufficiency and reasons for misinterpretations in angiography (author's transl)]. Different morphological and functional causes of the vertebrobasilar artery insufficiency, which were found in the examination of 128 patients, were analyzed and compared with well known syndromes. Possible misinterpretation in evaluation of angiograms are emphasized.


[Pheochromocytoma with scalloping of the ureter (author's transl)]. A case is described in which ureteral vascular impressions called "notching" or "scalloping sign" are caused by an ureteral artery supplying a pheochromocytoma. Vascular impressions on the ureter are recognized in different conditions. Most of them are due to collateral circulation following renal artery obstruction. The differential diagnosis is discussed.


Statistical tables to detect significance between frequencies in two small samples, with particular reference to biological assays. By simply referring to one of three tables, derived from Fisher's exact factorial method and obtained with the aid of a computer, it is possible to decide readily if differences observed between two small and equal or unequal size series are statistically significant at probability levels of 0.10, 0.05 and 0.01. The same can be very helpful for planning experiments. Examples are given in various fields of biology.


[Effects of histones on phagocytosis and phagocytic metabolism]. Enhanced phagocytosis and no intracellular glycogen breakdown was found in guinea-pig peritoneal polymorphonuclear leukocytes (PMN) incubated together with heat-killed staphylococci (Twort) treated with a calf thymus histone fraction (P II), for 1 hr at 37 degrees C. Glcose consumption and lactate production of these PMN's were as high as in phagocytosis of the same germ untreated with P II, and their oxygen consumption was depressed significantly.


[Influence of various enzyme inductors and combinations of inductors on DDT residue elimination in rats]. A study was under-taken to investigate the influence of pretreatments with various hepatic microsomal enzyme inducers on the elimination of DDT residues in rats previously contaminated with p,p' -DDT  at 5 mg/kg/day, during 20 days. The following inducers were used : phenobarbital (50 a mg/kg/day, i.p.), 3,4-benzopyrene (20 mg/kg/day, s.c.) and norethandrolone (20 mg/kg/day, s.c.), all given during 14 consecutive days. Each inducer was administered singly or in combination with the other two according to a 2 X 2 X 2 factorial experiment. The animals were then sacrificed for the measurement of p,p' -DDT, p,p' -DDD and p,p' -DDE residues in the blood, brain, abdominal fat, liver and kidneys. The results show that phenobarbital lowers markedly the concentration of total residues in the fat tissue and brain and that norethandrolone brings about a reduction of the residues in the blood, brain and kidneys, but not in the fat tissue. On the opposite, 3,4-benzopyrene produces an increase of the residues in the brain, liver and kidneys. Phenobarbital thus appears to be more efficacious than the other two inducers in facilitating the elimination of DDT residues from the fat tissue. In addition, it appears that under the experimental conditions used during this investigation, the elimination of DDT residues is not further accelerated by combining the inducers one with each other.


A recording procedure for chronic microelectrodes in the paralyzed cat. A method is described for repeated recording sessions of cortical thalamic units in the unanaesthetized but paralyzed animal. Because the recording chamber is miniaturized, it is well tolerated by the subject between sessions. The system also permits painless and stable immobilization of the head without the use of earbars, avoiding surgical preparation during the actual recording sessions and allowing long periods of recording. Between sessions the animals always display normal behavior.


Role of peripheral and central chemosensitive afferents in the control of depth and frequency of breathing. In anaesthetized cats the response to hypercapnia was studied during normoxia, hypoxia (increased peripheral chemosensitive afferents, IPA) and after carotid sinus denervation (decreased peripheral chemosensitive afferents, DPA) in terms of: (a) central output to the inspiratory muscles, (b) bulbopontine respiratory activity and (c) threshold-inhibition curve for termination of inspiration (Vr/Ti relationship). IPA increased, whereas DPA decreased the central output, as estimated from the rate of change of the subatmospheric pressure developed in the trachea during occlusion of the airways. IPA and DPA did not modify the bulbo-pontine rhythm, as estimated from the timing of occluded breaths (phasic vagal afferents nil), nor the Vt/Ti relationship, both of which have been shown to vary as a function of PACO2. It is concluded that peripheral chemosensitive afferents influence only the output of the respiratory centres, whereas central chemosensitive afferents influence also the bulbo-pontine respiratory rhythm and the threshold inhibition curve for termination of inspiration.


Gas exchange in air sacs: contribution to respiratory gas exchange in ducks. Air sac gas exchange was studied in ducks by measuring the rates of inert gas uptake and of O2 and CO2 equilibration in caudal thoracic air sac whose ventilation was prevented by surgival sealing of the ostia. The data were analyzed on a model incorporating three possible routes by which air sac gas could be exchanged with the surrounding tissue: (1) into the blood perfusing the air sac walls; (2) into the adjoining air sac via tissue membranes; (3) into the bronchial system of the lung via diffusion through lung tissue bordering upon the caudal thoracic air sac. Exchange rates of gases via the two latter paths were found to be small as compared with the first route. From application of model parameters to O2 and CO2 exchange in air sacs under physiological conditions the following conclusions were drawn: (1) the caudal thoracic air sac makes the major contribution to total gas exchange between air sacs and blood; (2) this exchange can account for less than 5% of total respiratory gas exchange; (3) the exchange is too small to account for the O2 and CO2 partial pressures in caudal thoracic air sacs of ducks. Other mechanisms like gas exchange in neopulmonic parabronchi, which conduct air to the caudal air sacs during inspiration or re-inspiration of dead space appear to play a more significant role in the deviation of O2 and CO2 partial pressures in the caudal air sacs from those in inspired air.


Mechanical activities of trachea as measured in vitro and in vivo. The phenomenon of spontaneous contractions as measured in vivo and in vitro (isolated guinea pig trachea) is described. In vitro contractility is defined in terms of active tension (AT), the maximum rate of tension development (dT/dt) and time to peak tension (TTP). The length-tension relationship was established for spontaneous contractions of isolated guinea pig trachea. The effect of the temperature on spontaneous activities was also studied. An increase in temperature from 37.5 to 41.5C produced an increase in the maximum rate of tension development (dT/dt( and a decrease in the time to peak tension (TTP). A comparable decrease in temperature, from 37.5 to 33.5C produced slight but not statistically significa-nt increases in active tension, dT/dt and TTP. Frequency of the spontaneous contractions varied directly with changes in temperature. Employing a micro-strain-gauge transducer a rhytmic change of tracheal diameter has been observed in vivo in both guinea pigs and rabbits. Administration of atropine and isoproterenol in those experiments transiently abolished the appearance of these rhythmic changes of tracheal diameter.