system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Acyltransferases, Bacillus subtilis, Bacterial Proteins, Biotechnology, Gene Expression Regulation, Bacterial, Heat-Shock Proteins, Heat-Shock Response, Lac Operon, Mutation, Periplasmic Proteins, Recombinant Fusion Proteins, Serine Endopeptidases, alpha-Amylases.
user
Is the Bacillus secretion stress response an indicator for alpha-amylase production levels? Context: Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at investigating a possible role of TepA in secretion stress management, and characterizing the intensity of the secretion stress response in relation to alpha-amylase production. The expression of a transcriptional htrB-lacZ gene fusion, and the levels of alpha-amylase production were monitored simultaneously using tepA mutant B. subtilis strains. TepA was shown to be dispensable for secretion stress management. Importantly, however, the levels of htrB-lacZ expression can be correlated with the levels of alpha-amylase production.
assistant
Our observations show that the secretion stress response can serve as an indicator for alpha-amylase production levels. Final Decision: yes