system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adolescent, Adult, Catalase, Exercise, Glutathione, Greece, Hematologic Tests, Humans, Male, Oxidative Stress, Protein Carbonylation, Research Design, Thiobarbituric Acid Reactive Substances, Time Factors.
user
Is sampling time crucial for measurement of aerobic exercise-induced oxidative stress? Context: To thoroughly investigate the time-course changes of several commonly used markers of oxidative stress by performing serial measurements during a 24-h period after an acute bout of strenuous cardiovascular exercise. Eleven untrained men performed two trials. In the experimental trial, the subjects exercised for 45 min at 70-75% VO2max and then at 90% VO2max to exhaustion on a treadmill; in the control trial, the subjects remained at rest. Blood samples were drawn before and after exercise (immediately after exercise and at 0.5, 1, 2, 3, 4, 5, 6, 8, 10, and 24 h). Reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG, thiobarbituric acid-reactive substances (TBARS), protein carbonyls, catalase activity, and total antioxidant capacity (TAC) were determined. The time to lowest concentration after exercise was 1.7 +/- 0.7 h (mean +/- SD) for GSH/GSSG, and the time to highest concentration after exercise was 1.2 +/- 0.6 h for TBARS, 4.4 +/- 0.5 h for protein carbonyls, 0.5 +/- 0.4h for catalase, and 2.2 +/- 0.9 h for TAC. The greatest change after exercise was -74 +/- 9% for GSH/GSSG, 129 +/- 29% for TBARS, 135 +/- 53% for protein carbonyls, 51 +/- 16% for catalase, and 24 +/- 10% for TAC.
assistant
There is no best time point applying to all markers for collecting blood samples after aerobic exercise. The optimum postexercise time points for blood collection in untrained individuals are immediately after exercise for catalase, 1 h for TBARS, 2 h for TAC, GSH, and GSSG, and 4 h after exercise for protein carbonyls. Final Decision: yes