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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Angiogenic Proteins, Animals, Blood Glucose, Capillaries, Cell Line, Cell Proliferation, Delayed-Action Preparations, Diabetes Mellitus, Experimental, Diabetic Angiopathies, Endothelial Cells, Hindlimb, Immunohistochemistry, Intercellular Signaling Peptides and Proteins, Ischemia, Laser-Doppler Flowmetry, Male, Mice, Mice, Inbred C57BL, Muscle, Skeletal, Neovascularization, Physiologic, Platelet-Rich Plasma, Random Allocation, Recovery of Function, Regional Blood Flow, Time Factors.
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Does therapeutic treatment with sustained-release platelet-rich plasma restore blood perfusion by augmenting ischemia-induced angiogenesis and arteriogenesis in diabetic mice? Context: The objective of this investigation was to establish the effectiveness of sustained-release platelet-rich plasma (PRP) on perfusion and neovascularization in diabetic murine hind limb ischemia. After surgery in streptozotocin-induced diabetic mice, the mice were randomly assigned to the following 4 experimental groups: control (C), 100 μl of the sustained-release form of platelet-poor plasma (PPP), 100 μl of the solution form of PRP (PRP-sol), and 100 μl of the sustained-release form of PRP (PRP-sr). Endpoint evaluations were: blood perfusion by laser Doppler perfusion imaging (LDPI), vascular density by anti-vWF, and mature vessel density by anti-smooth muscle actin antibody. This study demonstrated that a sustained release of PRP increases the perfusion of ischemic tissue as measured by LDPI (57 ± 12; 56 ± 9; 72 ± 7, and 98 ± 4 for the C, PPP, PRP-sol, and PRP-sr groups, respectively; p < 0.05), capillary density (151 ± 16; 158 ± 12; 189 ± 39, and 276 ± 39 for groups C, PPP, PRP-sol, and PRP-sr, respectively; p < 0.05), and mature vessel density (28 ± 2; 31 ± 3; 52 ± 10, and 85 ± 13 for the C, PPP, PRP-sol, and PRP-sr groups, respectively; p < 0.05).
assistant
A sustained release of PRP containing potent angiogenic growth factors restores blood perfusion by stimulating angiogenesis and arteriogenesis. Final Decision: yes