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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Cell Line, Tumor, Cell Proliferation, Gene Expression Regulation, Neoplastic, Humans, MicroRNAs, Real-Time Polymerase Chain Reaction, Receptor, IGF Type 1, Stomach Neoplasms.
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Does miR-494 inhibit invasion and proliferation of gastric cancer by targeting IGF-1R? Context: The aim of this study was to investigate the target gene of miR-494 and its roles in tumor growth of gastric cancer (GC). Expression of miR-494 was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) in gastric cancer tissues and cell lines. Then, luciferase reporter assay was used to elucidate whether insulin-like growth factor 1 receptor (IGF1R) is a target gene of miR-494. Finally, the roles and mechanism of miR-494 in the regulation of tumor invasion were further investigated. Relative miR-494 level was found to be significantly lower in patients with GC than healthy controls (p < 0.01). Over-expression of miR-494 could inhibit gastric cancer cell proliferation, migration, and invasion in vitro. Furthermore, we demonstrated that miR-494 binds to the 3'-untranslated region (UTR) of IGF1R and inhibits the expression of the IGF1R protein.
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Our data showed that miR-494 acted as a tumor suppressor in GC. Final Decision: yes