system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: 9,10-Dimethyl-1,2-benzanthracene, Animals, Anticarcinogenic Agents, Carcinogens, Cell Transformation, Neoplastic, Female, Flavones, HL-60 Cells, Humans, Mice, Skin Neoplasms, Tetradecanoylphorbol Acetate.
user
Does zapotin prevent mouse skin tumorigenesis during the stages of initiation and promotion? Context: Zapotin, a flavonoid associated with Casimiroa edulis, was isolated as part of a program to discover natural inhibitors of carcinogenesis. Zapote blanco, the fruit of Casimiroa edulis, is consumed in many parts of the world. Zapotin is a non-toxic inducer of cellular differentiation, apoptosis and cell cycle arrest with cultured HL-60 promyelocytic cells. An efficient chemical synthesis for zapotin was devised. Using this synthetic material, activity was examined in the two-stage mouse skin carcinogenesis model. Topical zapotin significantly inhibited 7,12-dimethylbenz(a)anthracene/12-O-tetradecanoylphorbol-13-acetate-induced mouse skin tumorigenesis, using the anti-initiation and anti-promotion protocols.
assistant
Encouraging results from previous and current in vivo studies warrant further investigation of the chemopreventive activity of zapotin. Final Decision: yes