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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: UNASSIGNED. Meshes: .
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Do primary Salivary Human Stem/Progenitor Cells Undergo Microenvironment-Driven Acinar-Like Differentiation in Hyaluronate Hydrogel Culture? Context: : Radiotherapy for head and neck cancer often has undesirable effects on salivary glands that lead to xerostomia or severe dry mouth, which can increase oral infections. Our goal is to engineer functional, three-dimensional (3D) salivary gland neotissue for autologous implantation to provide permanent relief. An immediate need exists to obtain autologous adult progenitor cells as the use of embryonic and induced pluripotent stem cells potentially pose serious risks such as teratogenicity and immunogenic rejection. Here, we report an expandable population of primary salivary human stem/progenitor cells (hS/PCs) that can be reproducibly and scalably isolated and propagated from tissue biopsies. These cells have increased expression of progenitor markers (K5, K14, MYC, ETV4, ETV5) compared with differentiation markers of the parotid gland (acinar: MIST1/BHLHA15 and AMY1A; ductal: K19 and TFCP2L1). Isolated hS/PCs grown in suspension formed primary and secondary spheres and could be maintained in long-term 3D hydrogel culture. When grown in a customized 3D modular hyaluronate-based hydrogel system modified with bioactive basement membrane-derived peptides, levels of progenitor markers, indices of proliferation, and viability of hS/PCs were enhanced. When appropriate microenvironmental cues were provided in a controlled manner in 3D, such as stimulation with β-adrenergic and cholinergic agonists, hS/PCs differentiated into an acinar-like lineage, needed for saliva production. We conclude that the stem/progenitor potential of adult hS/PCs isolated without antigenic sorting or clonal expansion in suspension, combined with their ability to differentiate into specialized salivary cell lineages in a human-compatible culture system, makes them ideal for use in 3D bioengineered salivary gland applications.
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Therapeutic irradiation for locally invasive head and neck cancers often leads to xerostomia or severe dry mouth, which greatly impairs quality of life. With current remedies being unsatisfactory, engineering an implantable salivary gland capable of restoring salivary functions of these patients is a promising solution. A major milestone toward salivary gland engineering is the identification and isolation of an expandable population of adult salivary human stem/progenitor cells (hS/PCs). Here we report a population of salivary hS/PCs that can be reliably isolated in scalable quantities, maintained long-term in two-dimensional/three-dimensional (3D) culture, and used to produce a variety of salivary epithelial cell types for use in our human-compatible, 3D engineered hydrogel model. Our ultimate goal is to use these cells to regenerate a functional autologous implantable salivary gland fully capable of reversing xerostomia. Final Decision: yes