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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Cells, Cultured, Conjunctiva, Cyclooxygenase Inhibitors, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Epithelial Cells, Histamine, Histamine Agonists, Humans, Interleukin-6, Interleukin-8, Niflumic Acid, Real-Time Polymerase Chain Reaction, Up-Regulation.
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Does niflumic acid reduce histamine-induced interleukin-6 and -8 expression in human conjunctival epithelial cells? Context: Histamine remains the main mediator of allergic conjunctivitis and induces interleukin (IL)-6 and IL-8 production in human conjunctival epithelial cells (HCEC). The purpose of the present study was to determine whether histamine induced IL-6 and IL-8 expression in HCEC, and to describe the relationship between human calcium-activated chloride channel (hCLCA) 1 activity and IL-6 and IL-8 expression. HCEC were isolated from human conjunctiva and cultured at an air-liquid interface. IL-6 and IL-8 mRNA expression was examined by real-time PCR, and IL-6 and IL-8 secretion was measured by ELISA after stimulation of HCEC with histamine. Histamine upregulated IL-6 and IL-8 expression and IL-6 and IL-8 secretion in a dose-dependent manner in HCEC. Niflumic acid (NFA), an hCLCA blocker, reduced histamine-induced IL-6 and IL-8 expression.
assistant
Histamine-induced IL-6 and IL-8 production could be attenuated by NFA, an hCLCA blocker. Final Decision: yes