system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, METHODS, METHODS, RESULTS. Meshes: Biological Factors, Blastocyst, Cell Line, Chorionic Gonadotropin, Culture Media, Conditioned, Embryonic and Fetal Development, Endometrium, Epithelial Cells, Female, Fertilization in Vitro, Homeodomain Proteins, Humans, Reverse Transcriptase Polymerase Chain Reaction, Solubility, Sperm Injections, Intracytoplasmic.
user
Does a soluble molecule secreted by human blastocysts modulate regulation of HOXA10 expression in an epithelial endometrial cell line? Context: To determine the existence of a soluble signal, secreted from the human blastocyst embryo, that induces HOXA10 gene expression before cell-cell contact. To analyze, by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), cell-free media that had contained human embryos cultured to the blastocyst stage for a soluble molecule that induces HOXA10 expression in an endometrial epithelial cell line (Ishikawa). Assisted reproduction technology program of Yale University, New Haven, Connecticut. Patients undergoing intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) cycles. Treatment of Ishikawa cells with blastocyst-conditioned media. Determination of HOXA10 gene expression. We demonstrate that cell-free media that had contained human embryos cultured to the blastocyst stage contain a soluble molecule that induces HOXA10 expression in an endometrial epithelial cell line (Ishikawa). We found that hCG does not induce HOXA10 in Ishikawa cells.
assistant
Soluble molecules induce a well-characterized marker of endometrial receptivity in endometrial cells without blastocyst apposition. Additionally, HOXA10 induction can serve as a means of evaluating human embryos cultured for IVF and ET. High quality embryos may induce local endometrial receptivity before trophectoderm-endometrial contact. Final Decision: yes