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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Animals, Blotting, Western, Brain Chemistry, Cattle, Cell Extracts, PrPSc Proteins, Rumen, Scrapie, Sheep, Domestic.
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Does exposure of sheep scrapie brain homogenate to rumen-simulating conditions result in a reduction of PrP ( Sc ) levels? Context: Experiments were designed to evaluate the potential of rumen-simulating conditions to reduce PrP(Sc) levels. Scrapie-positive brain material was incubated under rumen-simulating conditions. Time points were taken over a 24-h period and PrP(Sc) levels were analysed by Western blot. No loss of PrP(Sc) was observed over a 24-h time period.
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Our results indicate that a fully developed rumen fermentation does not provide significant protection against prion infection via the oral route. Developmental changes including senescence of immune system function or other developmental changes in the gastrointestinal tract are potential mechanisms by which relative bovine spongiform encephalopathy (BSE) susceptibility might vary with age. Final Decision: no