system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Female, Genetic Therapy, Hypoxia-Inducible Factor 1, alpha Subunit, Mice, Mice, Inbred C57BL, Neoplasms, Experimental, Neovascularization, Pathologic, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Transfection, Vascular Endothelial Growth Factor A, Von Hippel-Lindau Tumor Suppressor Protein.
user
Does [ Gene transfer of von Hippel-Lindau inhibit the growth of transplanted solid tumors ]? Context: To explore the effect of von Hippel-Lindau(VHL) gene on growth of EL-4 solid tumors in vivo. C57BL/6 mice model of solid tumors was established by subcutaneous injection of EL-4 lymphoma cells. Mice were randomly divided into two groups as treatment group (n=6) and control group (n=6) when tumor diameter increased to 0.1 cm and 0.4 cm respectively. Plasmid pcDNA3-VHL was injected into solid tumor in treatment group, empty pcDNA3 vector in control group. The growth of tumor was observed. Immunohistochemistry and Western blot analysis were used to examine the transgenic expression of VHL, hypoxia inducible factor-1alpha (HIF)-1alpha, bcl-2 and VEGF. Microvessel density (MVD) and apoptosis index (AI) of tumors were also detected. VHL gene transfer eradicated tumors with small size (0.1 cm diameter), but it only retarded the growth of large tumors (0.4 cm diameter). VHL was overexpressed, the expression levels of VEGF, HIF-1alpha and bcl-2 were reduced in treatment group compared with those in the control group. The level of MVD was significantly lower in treatment group (P< 0.05), but AI was higher in treatment group compared with those in the control group (P< 0.01).
assistant
VHL gene therapy can inhibit the growth of EL-4 solid tumor in vivo. Final Decision: yes