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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Acute Disease, Amylases, Animals, Cysteine Proteinase Inhibitors, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Leupeptins, Lung, Lung Diseases, Male, Organ Size, Pancreas, Pancreatitis, Peroxidase, Proteasome Endopeptidase Complex, Proteasome Inhibitors, Rats, Rats, Sprague-Dawley, Severity of Illness Index, Taurocholic Acid, Tumor Necrosis Factor-alpha.
user
Does proteasome inhibitor ameliorate severe acute pancreatitis and associated lung injury of rats? Context: To observe the effect of proteasome inhibitor MG-132 on severe acute pancreatitis (SAP) and associated lung injury of rats. Male adult SD rats were randomly divided into SAP group, sham-operation group, and MG-132 treatment group. A model of SAP was established by injection of 5% sodium taurocholate into the biliary-pancreatic duct of rats. The MG-132 group was pretreated with 10 mg/kg MG-132 intraperitoneally (ip) 30 min before the induction of pancreatitis. The changes in serum amylase, myeloperoxidase (MPO) activity of pancreatic and pulmonary tissue were measured. The TNF-alpha level in pancreatic cytosolic fractions was assayed with an enzyme-linked immunosorbent assay (ELISA) kit. Meanwhile, the pathological changes in both pancreatic and pulmonary tissues were also observed. MG-132 significantly decreased serum amylase, pancreatic weight/body ratio, pancreatic TNF-alpha level, pancreatic and pulmonary MPO activity (P < 0.05). Histopathological examinations revealed that pancreatic and pulmonary samples from rats pretreated with MG-132 demonstrated milder edema, cellular damage, and inflammatory activity (P < 0.05).
assistant
The proteasome inhibitor MG-132 shows a protective effect on severe acute pancreatitis and associated lung injury of rats. Final Decision: yes