system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: UNASSIGNED. Meshes: ATP Binding Cassette Transporter 1, Animals, Axons, Blood-Brain Barrier, Bone Marrow Cells, Capillaries, Cell Proliferation, Cell Survival, Diabetes Mellitus, Experimental, Diabetes Mellitus, Type 1, Gene Knockdown Techniques, Male, Mesenchymal Stem Cell Transplantation, Mesenchymal Stem Cells, MicroRNAs, Myocytes, Smooth Muscle, Neovascularization, Physiologic, Oligodendroglia, Rats, Wistar, Receptor, IGF Type 1, Stroke, Treatment Outcome, Vascular Remodeling, White Matter, Zonula Occludens-1 Protein.
user
Does miR-145 regulate Diabetes-Bone Marrow Stromal Cell-Induced Neurorestorative Effects in Diabetes Stroke Rats? Context: : In rats with type 1 diabetes (T1DM), the therapeutic effects and underlying mechanisms of action of stroke treatment were compared between bone-marrow stromal cells (BMSCs) derived from T1DM rats (DM-BMSCs) and BMSCs derived from normal rats (Nor-BMSCs). The novel role of microRNA-145 (miR-145) in mediating DM-BMSC treatment-induced benefits was also investigated. T1DM rats (n = 8 per group) underwent 2 hours of middle cerebral artery occlusion (MCAo) and were treated 24 hours later with the one of the following (5 × 10
assistant
In rats with type 1 diabetes (T1DM), the therapeutic effects and underlying mechanisms of action of stroke treatment were compared between bone-marrow stromal cells (BMSCs) derived from T1DM rats (DM-BMSCs) and BMSCs derived from normal rats (Nor-BMSCs). In vitro, DM-BMSCs and derived exosomes decreased miR-145 expression and increased DM-BMSC survival, capillary tube formation, and axonal outgrowth, compared with Nor-BMSCs; these effects were decreased by DM-BMSCs in which miR-145 was overexpressed. In vivo, compared with Nor-BMSC or phosphate-buffered saline treatment, DM-BMSC treatment improved functional outcome and vascular and white matter remodeling, decreased serum miR-145 expression, and increased expression of the miR-145 target genes ABCA1 and IGFR1. microRNA-145 mediated the benefits induced by DM-BMSC treatment. Final Decision: yes