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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, RESULTS. Meshes: Cell Line, Gene Library, Hepatitis B virus, Hepatocytes, Heterotrimeric GTP-Binding Proteins, Humans, Protein Binding, Protein Interaction Mapping, Protein Subunits, Trans-Activators, Two-Hybrid System Techniques, Viral Regulatory and Accessory Proteins.
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Does hepatitis B virus X protein interact with beta5 subunit of heterotrimeric guanine nucleotide binding protein? Context: To isolate cellular proteins interacting with hepatitis B virus X protein (HBX), from HepG2 cells infected with hepatitis B virus (HBV). HBV particles were produced in culture medium of HepG2 cells transfected with the mammalian expression vector containing the linear HBV genome, as assessed by commercially available ELISA assay. A cDNA library was made from these cells exposed to HBV. From yeast two hybrid screening with HBX as bait, human guanine nucleotide binding protein beta subunit 5L (GNbeta5) was isolated from the cDNA library constructed in this study as a new HBX-interacting protein. The HBX-GNbeta5 interaction was further supported by mammalian two hybrid assay.
assistant
The use of a cDNA library constructed from HBV-transfected HepG2 cells has resulted in the isolation of new cellular proteins interacting with HBX. Final Decision: yes