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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adolescent, Animals, Bone Matrix, Bone Morphogenetic Protein 2, Bone Regeneration, Bone Substitutes, Cells, Cultured, Craniotomy, Female, Fibroblasts, Gene Transfer Techniques, Genetic Therapy, Gingiva, Guided Tissue Regeneration, Humans, Implants, Experimental, Male, Osseointegration, Rats, Rats, Sprague-Dawley, Statistics, Nonparametric, Tissue Engineering, Tissue Scaffolds, X-Ray Microtomography.
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Does ex vivo bone morphogenetic protein-2 gene delivery using gingival fibroblasts promote bone regeneration in rats? Context: The aim of the present study was to investigate bone regeneration following ex vivo bone morphogenetic protein-2 (BMP-2) gene delivery using human gingival fibroblasts (HGFs) in rat calvarial defects. An 8 mm craniotomy defect was created in Sprague-Dawley rats. The animals were divided into four groups: (1) non-grafted group, the defect was left empty; (2) collagen matrix group, the defect was filled with collagen matrix only; (3) HGF group, the defect was filled with non-transduced HGFs on collagen matrix; (4) BMP-2/HGF group, the defect was filled with BMP-2 gene-transduced HGFs on collagen matrix. Animals were sacrificed at 2 and 4 weeks after surgery, and micro-computed tomographic and histologic observations were performed. The BMP-2/HGF group showed promoted osseous healing of calvarial defects, as compared with the other groups. At both 2 and 4 weeks, regenerated bone area was significantly greater in the BMP-2/HGF group than the other three groups. Quite a few number of transplanted HGFs were observed within the regenerated bone tissues.
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The results of this study suggest that ex vivo BMP-2 gene delivery induces prominent bone regeneration in vivo and HGFs may be useful as target cells for ex vivo gene therapy. Final Decision: yes