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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adult, B-Lymphocytes, Cell Proliferation, Female, Humans, Immunologic Memory, Pre-Eclampsia, Pregnancy, Prospective Studies, T-Lymphocytes, Regulatory.
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Are treg cells negatively correlated with increased memory B cells in pre-eclampsia while maintaining suppressive function on autologous B-cell proliferation? Context: The objective of this study was to determine the simultaneous changes and the correlation between circulating regulatory T (Tregs) and B-cell subsets in normal pregnancy (NP) and pre-eclampsia (PE). Meanwhile, the regulatory function of Tregs on B-cell proliferation was evaluated in vitro. A total of 32 PE and 40 NP women matched for age, gestational age, and parity were included. Using flow cytometry, the percentages of peripheral blood Tregs and B-cell subsets were determined. Moreover, the regulatory function of Tregs on autologous B-cell proliferation in PE and NP was investigated in vitro by flow cytometry and analyzed by modfit software. The proportions (%) of CD4(+)  Foxp3(+) (P < 0.001) and CD4(+)  CD25(+)  Foxp3(+) (P < 0.01) cells within total CD4(+) T cells were significantly decreased in PE as compared to those of NP. However, no difference was found in CD4(+)  CD25(+) cells in CD4(+) T cell population. CD19(+)  CD27(+) (P < 0.05) and CD19(+)  CD27(+)  IgD(+) (P < 0.01) B cells within CD19(+) B cells (%) in PE were higher than those of NP. Negative correlations between the percentage of CD4(+)  CD25(+)  Foxp3(+) in CD4(+) T cells and that of CD19(+)  CD27(+) (r = -0.384, P < 0.05) and CD19(+)  CD27(+)  IgD(+) (r = -0.402, P < 0.05) in CD19(+) B cells were present in PE but not in NP. Furthermore, the in vitro study demonstrated a significant increase in B-cell proliferation in PE as compared to NP. Tregs in PE were able to suppress the proliferation and precursor frequency of autologous B cells (P < 0.05 each).
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The study indicates that a negative correlation between Tregs and memory B cells is present in women with PE, characterized by a systemic decrease in Tregs and an increase in memory B cells. Although the quantitative deficit of Tregs is present in PE, the Tregs still have a suppressive role in autologous B-cell proliferation. Final Decision: yes