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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: Antioxidants, Biomarkers, Case-Control Studies, Child, Child, Preschool, DNA Damage, Deoxyguanosine, Diet, Female, Humans, Male, Oxidation-Reduction, Phenylalanine, Phenylketonurias, Risk Factors.
user
Is low total antioxidant status implicated with high 8-hydroxy-2-deoxyguanosine serum concentrations in phenylketonuria? Context: Phenylketonuria (PKU), an inborn error of metabolism, is treated with a low phenylalanine (Phe) lifelong diet, which can be characterized as vegetarian. 8-Hydroxy-2-deoxyguanosine (8-OHdG) is highly implicated in degenerative diseases. To evaluate the effect of plasma total antioxidant status (TAS) and Phe on the serum marker of DNA damage, 8-OHdG, in PKU. Twenty-four PKU patients on a strict diet (group A), 25 PKU patients on a "loose diet" (group B), and 24 healthy children (controls) participated in this study. Plasma TAS was evaluated spectrophotometrically. 8-OHdG and Phe were measured in blood with immunoassays. TAS levels were significantly higher (P < 0.001) in group A (1458 +/- 140 micromol/L) and controls (1452 +/- 235 micromol/L) than those in group B (907 +/- 150 micromol/L). In contrast, 8-OHdG serum levels were 2-fold higher in group B (0.22 +/- 0.03 ng/mL) as compared with those in group A (0.11 +/- 0.02 ng/mL) and 3-fold higher than those in controls (0.08 +/- 0.02 ng/mL) (P < 0.001). As expected, Phe levels were also significantly higher in group B than those in the other study groups. Positive correlation coefficients were found between Phe and 8-OHdG levels, whereas negative correlations were evaluated between TAS and 8-OHdG in all groups.
assistant
The high Phe and the low TAS plasma levels in PKU patients on a "loose diet" may induce DNA oxidation, as evidenced by the measured high 8-OHdG level in their sera. 8-OHdG evaluation may be a useful marker of increased risk for a neurodegenerative process. Final Decision: yes