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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Burns, Cicatrix, Male, Oligonucleotides, Antisense, Rats, Rats, Sprague-Dawley, Transforming Growth Factor beta, Transforming Growth Factor beta1, Wound Healing.
user
Do [ The experimental study on antisense TGF-beta 1 in inhibiting scar formation in III degree burn wound ]? Context: To investigate the effect of antisense TGF-beta 1 in inhibiting scar formation during wound healing. SD rats were divided into three groups after skin burn: group one was treated with antisense TGF-beta 1 oligonucleotide; group two was treated with antisense TGF-beta 1 recombinant plasmid and the control group. In different time, RT-PCR and immunohistochemistry were used to verify the expression of TGB beta 1 mRNA and protein. Type I Collagen mRNA expression was determined by hybridization in situ. Inflammatory reaction and collagen distribution were observed pathologically. In the groups received antisense ODN and recombinant plasmid, the expression of TGF-beta 1 mRNA and protein reduced during 14 days after burn. In the control group, type I collagen mRNA began to express at the second week and reached a peak at the fourth week, while the antisense groups kept low expression. The antisense group also showed mild inflammatory reaction and less synthesis of collagen.
assistant
Antisense TGF-beta 1 could prevent the scar formation during wound healing. Final Decision: yes