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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, RESULTS. Meshes: Acetylation, CREB-Binding Protein, Cell Cycle Proteins, Cell Proliferation, E1A-Associated p300 Protein, Gene Expression Regulation, Gene Knockdown Techniques, HEK293 Cells, HeLa Cells, Histones, Humans, Models, Biological, Nuclear Proteins, Phenotype, Promoter Regions, Genetic, Protein Binding, RNA Polymerase II, S Phase, Sirtuin 1, Transcription, Genetic.
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Are cBP/p300 and SIRT1 involved in transcriptional regulation of S-phase specific histone genes? Context: Histones constitute a type of essential nuclear proteins important for chromatin structure and functions. The expression of major histones is strictly confined to the S phase of a cell cycle and tightly coupled to DNA replication. With RT-qPCR and ChIP assays, we investigated transcriptional regulation of the S-phase specific histone genes and found that the acetylation level of histones on core histone gene promoters fluctuated during cell cycle in a pattern similar to RNA polymerase II association. Further, we showed that CBP/p300 and SIRT1 were recruited to histone gene promoters in an NPAT-dependent manner, knockdown of which affected histone acetylation on histone gene promoters and histone gene transcription.
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These observations contribute to further understanding of the mechanism by which the expression of canonical histone genes is regulated, and also implicate a link between histone expression and DNA damage repair and cell metabolism. Final Decision: yes