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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, METHODS, METHODS, METHODS, RESULTS. Meshes: Animals, Chromosomes, Cricetinae, Female, Meiosis, Mesocricetus, Metaphase, Oocytes, Organelles, Pregnancy.
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Does the first polar body predict accurately the location of the metaphase II meiotic spindle in mammalian oocytes? Context: To evaluate how well polar body location predicts spindle localization and to examine spindle morphology. Randomized, controlled animal study. University-affiliated research laboratory. Mature, female golden hamsters. After superovulation with pregnant mare serum gonadotropin and hCG, metaphase II oocytes were obtained and imaged under digital polarization microscopy. Identify the meiotic spindle in living, unfixed hamster oocytes and determine spindle location relative to the polar body. Spindles were imaged in 30 oocytes and only in 5 of them could the polar body predict the spindle localization. In the remaining oocytes, the spindles presented a random distribution within the cytoplasm.
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These data show that the polar body location is not an accurate predictor for meiotic spindle location in mammalian oocytes. Final Decision: no