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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, METHODS. Meshes: Animals, Autonomic Nervous System Diseases, Betamethasone, Disease Models, Animal, Female, Female Urogenital Diseases, Glucocorticoids, Gynecologic Surgical Procedures, Hypogastric Plexus, Perioperative Care, Rats, Rats, Sprague-Dawley, Urinary Bladder Diseases, Urologic Surgical Procedures.
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Does perioperative betamethasone treatment reduce signs of bladder dysfunction in a rat model for neurapraxia in female urogenital surgery? Context: Information on autonomic neurapraxia in female urogenital surgery is scarce, and a model to study it is not available. To develop a model to study the impact of autonomic neurapraxia on bladder function in female rats, as well as to assess the effects of corticosteroid therapy on the recovery of bladder function in this model. Female Sprague-Dawley rats were subjected to bilateral pelvic nerve crush (PNC) and perioperatively treated with betamethasone or vehicle. Bladder function and morphology of bladder tissue were evaluated and compared with sham-operated rats. Western blot, immunohistochemistry, organ bath experiments, and cystometry.
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Sham-operated rats exhibited regular micturitions without nonvoiding contractions (NVCs). Crush of all nerve branches of the pelvic plexus or PNC resulted in overflow incontinence and/or NVCs. Betamethasone treatment improved recovery of regular micturitions (87.5% compared with 27% for vehicle; p<0.05), reduced lowest bladder pressure (8 ± 2 cm H(2)O compared with 21 ± 5 cm H(2)O for vehicle; p<0.05), and reduced the amplitude of NVCs but had no effect on NVC frequency in PNC rats. Compared with vehicle, betamethasone-treated PNC rats had less CD68 (a macrophage marker) in the pelvic plexus and bladder tissue. Isolated bladder from betamethasone-treated PNC rats exhibited better nerve-induced contractions, contained more cholinergic and sensory nerves, and expressed lower amounts of collagen III than bladder tissue from vehicle-treated rats. Final Decision: yes