system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Amino Acid Chloromethyl Ketones, Anti-Infective Agents, Antimicrobial Cationic Peptides, Apoptosis, Barium Compounds, Caspase Inhibitors, Cell Size, Cell Survival, Chlorides, DNA Fragmentation, DNA-Binding Proteins, HL-60 Cells, Humans, Ion Transport, Membrane Potentials, Mitochondria, Peptides, Cyclic, Potassium.
user
Is efflux of potassium ion an important reason of HL-60 cells apoptosis induced by tachyplesin? Context: To investigate the role of intercellular potassium in tachyplesin-induced HL-60 cells apoptosis. The concentration of intercellular potassium, cell volume and mitochondrial membrane potential were examined by flow cytometry. The concentration of intercellular potassium reduced in a time-dependent manner in tachyplesin-treated HL-60 cells. In addition, the loss of mitochondrial membrane potential was tightly coupled with the shrinkage of cell volume. Different caspase inhibitors protected against DNA degradation but did not prevent the loss of HL-60 cell viability induced by tachyplesin. Ba2+, which was a kind of blocker of volume-regulatory K+ channels, increased the viability of tachyplesin-treated HL-60 cells and maintained mitochondrial membrane potential and cell volume.
assistant
Efflux of K+ was an important reason for apoptosis in tachyplesin-treated HL-60 cells. Efflux of K+ affected the viability of tachyplesin-treated HL-60 cells independent of the process of caspase activation. Final Decision: yes