system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: 11-beta-Hydroxysteroid Dehydrogenase Type 1, Cells, Cultured, Chorion, Female, Glucocorticoids, Humans, RNA, Messenger, Up-Regulation.
user
Do [ Glucocorticoids up-regulate human chorion 11beta-hydroxysteroid dehydrogenase type 1 ]? Context: To study the co-localization of glucocorticoid receptor and 11beta-hydroxysteroid dehydrogenase 1 (11beta-HSD1) and to investigate whether glucocorticoids regulate the reductase activity and expression of 11beta-HSD1. Immunohistochemical staining for 11beta-HSD1 in cultured primary human chorionic trophoblasts was performed. Radiometric conversion assay and Northern blot analysis were respectively used to observe the activity and mRNA expression of 11beta-HSD1. 11beta-HSD1 and GR were co-expressed in the same chorionic trophoblast. Both 11beta-HSD1 reductase activity and mRNA levels were increased by dexamethasone (10(-6) mol/L, 10(-7) mol/L) in the cultured chorionic trophoblasts, and the effects were blocked by GR antagonist RU486 (10(-6) mol/L).
assistant
By binding to GR, glucocorticoids induce the expression of 11beta-HSD1 by a possible intracrine mechanism. Final Decision: yes