system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Animals, Antineoplastic Agents, Phytogenic, Carcinoma, Hepatocellular, Genistein, Hep G2 Cells, Humans, JNK Mitogen-Activated Protein Kinases, Liver Neoplasms, Matrix Metalloproteinase 9, Mice, Mice, Nude, NF-kappa B, Neoplasm Invasiveness, Phosphatidylinositol 3-Kinases, Phosphorylation, Phytotherapy, Plant Extracts, Signal Transduction, Soybeans, Tetradecanoylphorbol Acetate, Transcription Factor AP-1.
user
Does genistein inhibit tumor invasion by suppressing multiple signal transduction pathways in human hepatocellular carcinoma cells? Context: Genistein (Gen) exhibits anti-mutagenic and anti-metastatic activities in hepatoma cell lines. Gen has suppressive effects on tumor growth and angiogenesis in nude mice. Gen suppresses the enzymatic activity of matrix metalloproteinase (MMP)-9; however, the mechanism underlying its anti-invasive activity on hepatocellular carcinoma (HCC) cells is unclear. In this study, the possible mechanisms underlying Gen-mediated reduction of 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced cell invasion and inhibition of secreted and cytosolic MMP-9 production in human hepatoma cells (HepG2, Huh-7, and HA22T) and murine embryonic liver cells (BNL CL2) were investigated. Gen suppressed MMP-9 transcription by inhibiting activator protein (AP)-1 and nuclear factor-κ B (NF-κB) activity. Gen suppressed TPA-induced AP-1 activity through inhibitory phosphorylation of extracellular signal-related kinase (ERK) and c-Jun N-terminal kinase (JNK) signaling pathways, and TPA-stimulated inhibition of NF-κB nuclear translocation through IκB inhibitory signaling pathways. Moreover, Gen suppressed TPA-induced activation of ERK/phosphatidylinositol 3-kinase/Akt upstream of NF-κB and AP-1.
assistant
Gen and its inhibition of multiple signal transduction pathways can control the invasiveness and metastatic potential of HCC. Final Decision: yes