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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Caspases, Cell Proliferation, Colonic Neoplasms, Flow Cytometry, Gamma Rays, Gene Expression Profiling, Gene Knockdown Techniques, Humans, Immunoblotting, In Vitro Techniques, Mice, Mice, Nude, Oligonucleotide Array Sequence Analysis, RNA, Messenger, RNA, Small Interfering, Reverse Transcriptase Polymerase Chain Reaction, TNF-Related Apoptosis-Inducing Ligand, Taxoids, Tumor Cells, Cultured, X-Linked Inhibitor of Apoptosis Protein, Xenograft Model Antitumor Assays.
user
Are stable XIAP knockdown clones of HCT116 colon cancer cells more sensitive to TRAIL , taxanes and irradiation in vitro? Context: To develop a model of X-linked inhibitor of apoptosis (XIAP) down regulation in colorectal cancer cell lines. This may be used to determine whether combination strategies have clinical potential. A series of clones were developed using short hairpin RNA (shRNA) against XIAP stably expressed in HCT116 cells. XIAP mRNA and protein levels were established by RT-PCR and Immunoblot, respectively. GeneChip microarrays confirmed XIAP knockdown and absence of compensation by other IAP members. Four XIAP knockdown cell lines show 82-93% reduction in XIAP mRNA and 67-89% reduction in protein when compared to four luciferase control cell lines. XIAP knockdown sensitises cells to rhTRAIL by a factor of 3, to paclitaxel and docetaxel by a factor of >2 and, to a lesser extent, radiotherapy (20% enhancement).
assistant
Clinical trials with XIAP antisense continue, and these data suggest combination studies with agents such as rhTRAIL and taxanes should be undertaken. Final Decision: yes