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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Amino Acid Sequence, Bacterial Adhesion, Bacteriological Techniques, Base Sequence, Colon, DNA Primers, Electrophoresis, Polyacrylamide Gel, Glyceraldehyde-3-Phosphate Dehydrogenases, Humans, Intestinal Mucosa, Lactobacillus plantarum, Molecular Sequence Data, Mucins, Polymerase Chain Reaction, Probiotics, Sequence Homology, Amino Acid.
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Does cell surface Lactobacillus plantarum LA 318 glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) adhere to human colonic mucin? Context: To characterize the adhesion molecule of Lactobacillus plantarum LA 318 that shows high adhesion to human colonic mucin (HCM). The adhesion test used the BIACORE assay where PBS-washed bacterial cells showed a significant decrease in adherence to HCM than distilled water-washed cells. A component in the PBS wash fraction adhered to the HCM and a main protein was detected as a c. 40-kDa band using SDS-PAGE. Using homology comparisons of the N-terminal amino acid sequences compared with sequence databases, this protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The DNA sequence of LA 318 GAPDH was 100% identical to the GAPDH (gapB) of L. plantarum WCFS1. The purified GAPDH adhered to HCM.
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We found the adhesin of L. plantarum LA 318 to HCM in its culture PBS wash fraction. The molecule was identified as GAPDH. Because LA 318 possesses the same adhesin as many pathogens, the lactobacilli GAPDH may compete with pathogens infecting the intestine. Final Decision: yes