system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Apoptosis, Capsid Proteins, Cell Line, Tumor, Chicken anemia virus, Disease Models, Animal, Humans, Mice, Mice, Inbred BALB C, Neoplasms, Newcastle disease virus, Oncolytic Virotherapy, Oncolytic Viruses, Recombinant Proteins, Treatment Outcome, Virulence, Virulence Factors.
user
Does apoptin enhance the oncolytic properties of Newcastle disease virus? Context: Naturally occurring strains of Newcastle disease virus (NDV) have demonstrated the potential to kill cancer cells in both preclinical and clinical studies. Previous studies showed that apoptin, the VP3 protein of chicken infectious anemia virus, is a p53-independent, Bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis in transformed cells. In this study, we tested the hypothesis that apoptin enhances NDV-mediated tumor cell death. Reverse genetics was used to engineer an oncolytic NDV strain, FMW, to express apoptin. The antitumor effects of the recombinant virus (rFMW/AP) were also evaluated in the tumor cell lines and tumor-bearing mice. Compared to the parental strain FMW, rFMW/AP was more potent in killing A459 and SMMC7721 tumor cells. Recombinant NDV also exhibited higher efficacy in suppressing tumor growth in mice bearing A549-induced tumors. Furthermore, rFMW/AP did not display apparent toxic effects in either normal cells or control mice.
assistant
Our results suggest that the recombinant NDV expressing apoptin is a promising novel antitumor agent. Final Decision: yes