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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: 3' Untranslated Regions, Binding Sites, Carcinoma, Squamous Cell, Cell Line, Tumor, Cell Movement, Cell Proliferation, Esophageal Neoplasms, Esophageal Squamous Cell Carcinoma, Female, Gene Expression Regulation, Neoplastic, Humans, Lymphatic Metastasis, Male, MicroRNAs, Middle Aged, Neoplasm Invasiveness, Neoplasm Staging, Transcription Factors, Transfection, Up-Regulation.
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Does microRNA-1290 promote esophageal squamous cell carcinoma cell proliferation and metastasis? Context: To investigate the biological role of miR-1290 in esophageal squamous cell carcinoma (ESCC) progression and invasion and the underlying mechanism. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate miR-1290 expression in ESCC tissue samples. The roles of miR-1290 in cell proliferation, migration and invasion were identified using miR-1290 mimic-transfected cells. In addition, the regulatory effect of miR-1290 on suppressor of cancer cell invasion (SCAI) was evaluated using qRT-PCR, Western blot analysis and a dual luciferase reporter assay. miR-1290 was significantly upregulated in ESCC tissue samples compared with normal adjacent tissues (9.213 ± 1.150 vs 1.000 ± 0.0), (P < 0.01). Upregulation of miR-1290 was associated with tumor differentiation (P = 0.021), N classification (P = 0.006) and tumor-node-metastasis stage (P = 0.021) in ESCC patients. Moreover, ectopic miR-1290 expression potently promoted ESCC cell growth (P < 0.01), migration (P < 0.01) and invasion (P < 0.01) in vitro. miR-1290 overexpression in ESCC cell lines decreased SCAI expression at the translational level and reduced SCAI-driven luciferase-reporter activity (P < 0.01).
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Our findings suggested that miR-1290 may play an oncogenic role in cellular processes of ESCC. Final Decision: yes