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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: UNASSIGNED, OBJECTIVE, METHODS, RESULTS. Meshes: 1-Butanol, Animals, Arginase, Dose-Response Relationship, Drug, Enzyme Inhibitors, Leishmania, Macrophages, Mice, Ornithine, Parasitic Sensitivity Tests, Phytotherapy, Plant Extracts, Plants, Medicinal, Protozoan Proteins, RAW 264.7 Cells, Solvents, Trypanocidal Agents, Verbenaceae.
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Does stachytarpheta cayennensis extract inhibit promastigote and amastigote growth in Leishmania amazonensis via parasite arginase inhibition? Context: Stachytarpheta cayennensis is a plant that is traditionally used to treat tegumentary leishmaniasis and as an anti-inflammatory agent. This study aimed to evaluate the action of S. cayennensis extracts on the Leishmania (Leishmania) amazonensis arginase enzyme. S. cayennensis was collected from the Brazilian Amazon region. Aqueous extracts were fractionated with n-butanol. The leishmanicidal effects of the n-butanolic fraction (BUF) were evaluated in L. (L.) amazonensis promastigotes and amastigotes. BUF was tested against recombinant arginase from both L. (L.) amazonensis and macrophage arginase. Promastigote cultures and infected macrophage cultures were supplemented with L-ornithine to verify arginase inhibition. NMR analysis was used to identify the major components of BUF. BUF showed an EC
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Leishmanicidal effects of the S. cayennensis BUF fraction on L. (L.) amazonensis are associated with selective parasite arginase inhibition. Final Decision: yes