system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Cytokines, Dose-Response Relationship, Drug, Dose-Response Relationship, Immunologic, Drug Resistance, Encephalomyelitis, Autoimmune, Experimental, Female, Guinea Pigs, Male, Methylprednisolone, Nuclear Proteins, Protein Isoforms, RNA-Binding Proteins, Rats, Rats, Wistar, Receptors, Glucocorticoid, Serine-Arginine Splicing Factors, Treatment Outcome.
user
Does super-high-dose methylprednisolone improve efficacy or induce glucocorticoid resistance in experimental allergic encephalomyelitis? Context: To investigate whether a super-high dose (SHD) of methylprednisolone (MP) improves its efficacy or induces glucocorticoid (GC) resistance, and to explore the potential mechanisms of GC resistance in experimental allergic encephalomyelitis (EAE). The therapeutic effects of SHD and low-dose MP were evaluated in EAE by analyzing clinical scores, pathological changes and cytokine production. Immunohistochemistry and RT-PCR were used to investigate the expression of GC receptor (GR) isoforms and splicing factor SRp30c. Both MP doses had similar therapeutic effects. The ratio of GRα to GRβ was positively correlated with clinical score changes. However, there was no difference in the GRα/GRβ ratio between SHD and low-dose MP groups. SRp30c mRNA was correlated with GRβ expression.
assistant
This study indicates that the GRα/GRβ ratio is associated with GC sensitivity, and SRp30c may play an important role in promoting alternative splicing of GR pre-mRNA to generate GRβ in EAE rats. Compared with low-dose MP, SHD MP does not improve efficacy or induce GC resistance. Final Decision: no