system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Aging, Amides, Animals, Aorta, Ascorbic Acid, Calcium-Binding Proteins, Coronary Vasospasm, Coronary Vessels, Electrocardiography, Endothelium, Vascular, Enzyme Inhibitors, Hydrogen Peroxide, Intracellular Signaling Peptides and Proteins, Mice, Mice, Knockout, Nitric Oxide, Oxidation-Reduction, Oxidative Stress, Pyridines, Reactive Oxygen Species, Serotonin, Serotonin Receptor Agonists, Sulfhydryl Compounds, Vasoconstriction, Vasodilation, rho-Associated Kinases.
user
Does age-related oxidant stress with senescence marker protein-30 deficiency play a pivotal role in coronary artery spasm? Context: We examined the mechanism of coronary artery spasm related to oxidant stress with aging in senescence marker protein-30 (SMP30)-deficient mice because SMP30 decreases with aging and SMP30 knockout (KO) mice show a short life with increased oxidant stress. To examine the effect of SMP30 on coronary artery vasomotor tone, we measured the endothelium-dependent [5-hydroxytryptamine (5-HT)] response of isolated, pressurized coronary arteries from SMP30 KO and wild-type (WT) mice (n=10 each). In SMP30 KO mice, 5-HT-induced vasoconstriction occurred, which altered vasodilation with dithiothreitol, a thiol-reducing agent. In WT mice, 5-HT-induced vasodilation occurred. Administration of 5-HT from the aortic sinus induced a coronary artery spasm in SMP30 KO mice, which was prevented by the intravenous administration of Y-27632, rho-kinase inhibitor. The fluorescence level of monochlorobimane in coronary arteries, which covalently labels the reduced total thiols, decreased in SMP30 KO mice, but reverted to a level comparable with that of WT mice on treatment with Y-27632. From these results, SMP30 provides protection against coronary artery spasm.
assistant
Chronic oxidant stress associated with aging plays an important role in coronary artery spasm related to thiol oxidation and rho-kinase signaling. Final Decision: yes