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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Blotting, Northern, Blotting, Western, Endogenous Retroviruses, Gene Products, env, Humans, MicroRNAs, Neoplasms, Germ Cell and Embryonal, Prognosis, Transcriptome.
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Does expression of human endogenous retrovirus-K coincide with that of micro-RNA-663 and -638 in germ-cell tumor cells? Context: The cell line GH was established from germ-cell tumor tissue; human endogenous retrovirus-K (HERV-K) expression was detectable after prolonged culture of the cells, particularly in cells that formed domes and vesicles. In addition, keeping GH cells in culture at high cell densities increased HERV-K expression. Here, we studied whether this inducible HERV-K expression is accompanied by differences in microRNA (miRNA) expression patterns of GH cells. The global miRNA expression pattern of GH cell samples (HERV-K high versus low) was analyzed by miRNA arrays. Two miRNAs were found to be differentially regulated and to exhibit expression parallel to that of HERV-K. The identified miRNAs-663 and -638, have been reported to be involved in multiple processes, including cellular senescence. However, induction of HERV-K expression did not change the cellular senescence status of GH cells.
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The expression of these two miRNAs might be useful as novel diagnostic and prognostic markers in patients with tumors. Final Decision: yes