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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Apoptosis, Bisbenzimidazole, Cell Cycle, Cell Growth Processes, Cell Survival, Cells, Cultured, Chemokine CCL2, Female, Leptin, Male, Mice, Microscopy, Fluorescence, Myocytes, Cardiac, RNA, Messenger, Rats, Rats, Sprague-Dawley, Receptors, Cell Surface, Receptors, Leptin, Reverse Transcriptase Polymerase Chain Reaction.
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Does leptin induce hypertrophy , cell cycle alterations , or production of MCP-1 in cultured rat and mouse cardiomyocytes? Context: Leptin has been proposed as an important mediator in cardiovascular pathophysiology. Patients with congestive heart failure (CHF) present high plasma leptin levels. CHF is generally preceded by myocardial remodeling involving hypertrophy, necrosis, and apoptosis. To investigate whether leptin causes hypertrophy or cell cycle alterations, or induces MCP-1 synthesis in cardiomyocytes. Primary cultures of neonatal rat cardiomyocytes (PC) and murine cell line HL-1 were used. RT-PCR was used to identify Ob-Rb gene expression. Metabolic activity and proliferation of cardiomyocytes was studied using MTT and BrdU uptake, while apoptosis was assayed with Hoechst dye vital staining and flow cytometry. Measurement of the cell surface area was used to determine hypertrophy. MCP-1 levels were measured by ELISA. RT-PCR showed Ob-Rb mRNA expression in HL-1 cells. Exposure to leptin induced no changes in metabolic activity, proliferation, and apoptotic rates, and did not alter cell cycle in cardiomyocytes. Leptin did not increase cell size of cardiomyocytes, and MCP-1 synthesis was not detected in PC and HL-1 cells treated with leptin.
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This work shows that leptin does not induce changes in viability, proliferation, size or apoptosis of rat neonatal and HL-1 cardiomyocytes, and it does not induce MCP-1 secretion in these cells. Final Decision: no