system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Apoptosis, Blotting, Western, Cell Line, Tumor, Cell Transformation, Neoplastic, Colorectal Neoplasms, DNA Repair, Fluorescent Antibody Technique, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, HCT116 Cells, Humans, Immunoprecipitation, Microscopy, Confocal, Oligonucleotide Array Sequence Analysis, Phosphotransferases, Protein Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins p21(ras), RNA, Small Interfering, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Transfection, ras Proteins.
user
Is aLPK2 crucial for luminal apoptosis and DNA repair-related gene expression in a three-dimensional colonic-crypt model? Context: Oncogenic KRAS signaling is dysregulated in a three-dimensional (3D)-specific manner in human colorectal cancer (CRC) HCT116 cells. However, the identity of the crucial genes which are down-regulated through oncogenic KRAS in 3D cultures remains unclear. We established a specific anti-alpha-kinase 2 (ALPK2) antibody and addressed the ALPK2 function in HKe3 cells, which are HCT116 cells with a disruption in oncogenic KRAS, in a 3D colonic-crypt model. In HKe3 cells grown in 3D culture, ALPK2 siRNA inhibited luminal apoptosis and reduced the expression of cleaved caspase-3. Furthermore, ALPK2 siRNA reduced the expression of DNA repair genes. Reduced expression of ALPK2 mRNA was found to be correlated with clinical colorectal adenomas in a public dataset of gene expression analyses.
assistant
ALPK2, down-regulated by oncogenic KRAS, is crucial for luminal apoptosis and expression of DNA repair-related genes, possibly in the transition of normal colonic crypt to adenoma. Final Decision: yes