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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Animals, Blotting, Western, Bungarotoxins, Cell Line, Female, Fibronectins, Fluorescent Antibody Technique, Immunohistochemistry, Macrophage Activation, Macrophages, Mice, Mice, Inbred C57BL, Mice, Knockout, Nicotine, Paracrine Communication, Pregnancy, Prenatal Exposure Delayed Effects, Pulmonary Alveoli, Real-Time Polymerase Chain Reaction, Receptors, Nicotinic, Smoking, alpha7 Nicotinic Acetylcholine Receptor.
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Does in utero nicotine exposure promote M2 activation in neonatal mouse alveolar macrophages? Context: Maternal smoking in utero has been associated with adverse health outcomes including lower respiratory tract infections in infants and children, but the mechanisms underlying these associations continue to be investigated. We hypothesized that nicotine plays a significant role in mediating the effects of maternal tobacco smoke on the function of the neonatal alveolar macrophage (AM), the resident immune cell in the neonatal lung. Primary AMs were isolated at postnatal day 7 from a murine model of in utero nicotine exposure. The murine AM cell line MH-S was used for additional in vitro studies. In utero nicotine increased interleukin-13 and transforming growth factor-β1 (TGFβ1) in the neonatal lung. Nicotine-exposed AMs demonstrated increased TGFβ1 and increased markers of alternative activation with diminished phagocytic function. However, AMs from mice deficient in the α7 nicotinic acetylcholine receptor (α7 nAChR) had less TGFβ1, reduced alternative activation, and improved phagocytic functioning despite similar in utero nicotine exposure.
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In utero nicotine exposure, mediated in part via the α7 nAChR, may increase the risk of lower respiratory tract infections in neonates by changing the resting state of AM toward alternative activation. These findings have important implications for immune responses in the nicotine-exposed neonatal lung. Final Decision: yes