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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS. Meshes: B-Lymphocytes, Base Sequence, Butyrates, Coculture Techniques, DNA Primers, DNA, Viral, Gene Expression Regulation, Viral, Genes, Viral, Herpesviridae Infections, Herpesvirus 8, Human, Humans, Male, Phorbol Esters, Polymerase Chain Reaction, Prostatic Neoplasms, RNA, Messenger, RNA, Viral, Tumor Cells, Cultured, Viral Proteins, Virus Latency.
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Does hHV-8 ( KSHV ) establish latency in prostate cancer cell lines? Context: HHV-8 is a new herpesvirus found in lesions of Kaposi's sarcoma and some lymphoproliferative diseases. More recently, a report stated that normal prostate tissue also contains the virus. The expression of HHV-8 was examined by a sensitive reverse-transcriptase PCR for the viral genes ORF 72, ORF 73, ORF 74, and ORF 75. In coculture experiments we attempted to infect 3 commonly studied prostate cancer cell lines using induced and uninduced lymphoid cell lines harboring HHV-8 (KS-1, BC-1, and BC-2). For induction of viral genes, butyrate and phorbol esters were used.
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At baseline, prostate cancer cell lines LNCaP, DU-145, and PC-3 did not express viral gene products. Extensive coculture experiments were also negative. In no instance could latency for the virus be established. Our results argue against the involvement of HHV-8 in prostate cancer and for a limited tissue tropism of HHV-8. Final Decision: no