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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, RESULTS. Meshes: Animals, Bronchoalveolar Lavage Fluid, Chemotaxis, Leukocyte, Electrophoresis, Polyacrylamide Gel, Interleukin-8, Lung, Neutrophil Infiltration, Neutrophils, Rabbits, Recombinant Proteins, alpha-Macroglobulins.
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Does specific binding of IL-8 to rabbit alpha-macroglobulin modulate IL-8 function in the lung? Context: The purpose of this study was to compare chemotactic activity of IL-8 alone with that of IL-8 reacted with rabbit alpha-macroglobulins (alphaM) in vivo. Initially the binding of recombinant human IL-8 (rhIL-8) to rabbit alphaM was studied. 125I-labeled rhIL-8 was incubated with alphaM, and electrophoresed on native 5% gels or SDS-polyacrylamide 4-20% gradient gels. Next, rhIL-8 or rhIL-8 bound to alphaM was administered via an endotracheal tube to rabbit's lungs. An endotracheal tube was wedged into a segment of the lobe of each lung, and a sample instilled through the tube into this segment. After 4 h the lungs were lavaged. rhIL-8 bound to alphaM retained its full chemotactic activity in vitro but exhibited a diminished ability to induce the influx of neutrophils into the rabbit lung.
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The data suggest that alphaM may facilitate IL-8 clearance from the lung. Final Decision: yes