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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Amino Acid Sequence, Carrier Proteins, Chlorophyll, Chromosome Segregation, Cloning, Molecular, Disease Resistance, Expressed Sequence Tags, Fluorescence, Gene Expression Regulation, Plant, Genes, Plant, Genetic Linkage, Genotype, Molecular Sequence Data, Photosynthesis, Photosystem II Protein Complex, Phylogeny, Physical Chromosome Mapping, Plant Diseases, Plant Leaves, Plant Proteins, Polymerase Chain Reaction, Polymorphism, Genetic, Pseudomonas syringae, Real-Time Polymerase Chain Reaction, Seedlings, Triticum.
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Does wheat WCBP1 encode a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence? Context: Stripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence. We used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags. In silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.
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Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection. Final Decision: yes