system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adult, Aged, Aged, 80 and over, Animals, Antigens, CD, Cadherins, Carcinogenesis, Carcinoma, Squamous Cell, Cell Enlargement, Cell Line, Tumor, Diamines, Epithelial-Mesenchymal Transition, Female, Head and Neck Neoplasms, Humans, Lymphatic Metastasis, Male, Mice, Nude, Middle Aged, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasm Transplantation, Prognosis, Proto-Oncogene Proteins c-myc, Receptor, Notch1, Squamous Cell Carcinoma of Head and Neck, Thiazoles, Vimentin, Young Adult.
user
Does notch1 regulate invasion and metastasis of head and neck squamous cell carcinoma by inducing EMT through c-Myc? Context: As 50% of patients of head and neck squamous carcinoma (HNSCC) exhibit poor prognosis, the identification of new therapeutic targets is required. Recently, there have been several reports about the correlation between Notch1 and HNSCC, but the precise mechanism is still obscure. Therefore, in this study, we examined the involvement of Notch1 in HNSCC by using HNSCC cell lines and surgical specimens. To investigate the role of Notch1 in HNSCC, we examined the effect of Notch inhibitor DAPT on cell growth, invasion, and tumorigenicity using five HNSCC cell lines in vitro and in vivo. We further examined that the correlation with Notch expression and clinical prognostic factors was evaluated by using 101 HNSCC surgical specimens. DAPT reduced the nuclear expression of Notch and c-Myc and repressed cell growth, EMT-dependent cell invasion in vitro, and tumorigenicity in vivo. An overexpression of Myc enhanced EMT with an increase of Snail and vimentin together with decreased levels of E-cadherin in HSC3 cells. Finally, we discovered that Notch expression was well correlated with MIB-1 index and lymph node metastases.
assistant
We discovered that Notch1 was strongly correlated with HNSCC growth, invasion, and metastases. Therefore, Notch1 might be a new therapeutic target and a predictive marker of proliferation and metastasis of HNSCC. Final Decision: yes