system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Bronchopulmonary Dysplasia, Cell Adhesion, Cell Line, Tumor, Cell Movement, Cell Proliferation, Female, Fibronectins, Gene Expression Regulation, Humans, Lung, Male, Mice, MicroRNAs, RNA, Small Interfering, Wound Healing.
user
Does reduction of microRNA-206 contribute to the development of bronchopulmonary dysplasia through up-regulation of fibronectin 1? Context: To characterize microRNA-206 (miR-206) in the development of bronchopulmonary dysplasia (BPD). We assessed the expression of miR-206 in BPD mouse lung tissues and blood samples of BPD patients by quantitative real-time PCR. Then, the role of miR-206 in regulating cell biology were examined by XTT assay, flow cytometry, transwell invasion assay, wound healing assay and adhesion assay in vitro. Furthermore, luciferase reporter assay, real-time PCR, western blot and Immunofluorescence staining were performed to figure out the target gene of miR-206. A reduction in expression of miR-206 was observed in BPD mice compared with controls and in BPD patients compared with controls. miR-206 overexpression significantly induced cell apoptosis, reduced cell proliferation, migration and adhesion abilities, whereas the inhibition of miR-206 expression had the opposite effect. Fibronectin 1 (FN1) is a direct target of miR-206, and fn 1 can be transcriptionally and translationally regulated by miR-206. Down-regulation of miR-206 modulates biological functions of the cells, at least in part, by increasing the level of fn 1. Furthermore, fn 1 expression levels were increased in the BPD mice and BPD patients.
assistant
The expression of miR-206 and its target gene, fn 1, may contribute to the progression of BPD. Final Decision: yes