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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: Angelica sinensis, Animals, Annexin A5, Apoptosis, Astragalus Plant, Body Weight, Bone Marrow, Caspase 3, Cell Line, Disease Models, Animal, Drugs, Chinese Herbal, Hematopoiesis, Intracellular Membranes, Male, Megakaryocytes, Mice, Mice, Inbred BALB C, Mitochondria, Organ Size, Phytotherapy, Plant Extracts, Plant Roots, Platelet Count, Radiation Injuries, Experimental, Stem Cells, Thrombopoiesis, Thrombopoietin.
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Does an herbal decoction of Radix astragali and Radix angelicae sinensis promote hematopoiesis and thrombopoiesis? Context: A decoction containing Radix angelicae sinensis and Radix astragali (Danggui Buxue Tang, DBT) has been used to raise the "Qi" and nourish the "Blood". However, its effects on haematopoiesis and particularly thrombopoiesis have not been studied. This study aims to examine the effects of DBT on hematopoiesis and thrombopoiesis. A myelosuppression mouse model was treated with DBT (10mg/kg/day). Peripheral blood cells from DBT and thrombopoietin-treated samples were counted on days 0, 7, 14 and 21. Then CFU assays were used to determine the effects of DBT on the megakaryocytic progenitor cells and other lineages. Last, analyses of annexin V, caspase-3, and mitochondrial membrane potential were conducted in megakaryocytic cell line M-07e. Morphological examination showed that DBT treatment significantly increased the recovery of the megakaryocytic series. DBT significantly enhanced the platelet recovery and CFU-MK formation in vivo. DBT significantly promoted CFU-MK and CFU-F formation. Last, we observed the antiapoptotic effects of DBT on M-07e cells.
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DBT might promote haematopoiesis and thrombopoiesis in the mouse model through (i) directly promoting the growth of megakaryocytes; (ii) indirectly promoting the growth of bone marrow stromal cells; (iii) inhibiting apoptosis of megakaryocytes. Final Decision: yes