system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE. Meshes: Adult, Calcium Signaling, Cryopreservation, Female, Fertilization, Freezing, Fura-2, Humans, In Vitro Oocyte Maturation Techniques, Male, Oocytes, Sperm Injections, Intracytoplasmic, Vitrification.
user
Do oocyte cryopreservation and in vitro culture affect calcium signalling during human fertilization? Context: What are the precise patterns of calcium oscillations during the fertilization of human oocytes matured either in vivo or in vitro or aged in vitro and what is the effect of cryopreservation?
assistant
Human oocytes matured in vivo exhibit a specific pattern of calcium oscillations, which is affected by in vitro maturation, in vitro ageing and cryopreservation. Final Decision: yes