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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Acute Disease, Adenocarcinoma, Adenoviridae, Animals, Antigens, Neoplasm, Apoptosis, Biomarkers, Tumor, Blotting, Western, Cell Proliferation, Colitis, Ulcerative, Colon, Colonic Neoplasms, Disease Models, Animal, Gene Expression Profiling, Hydrogen Peroxide, Immunoenzyme Techniques, Inflammation, Interleukin-6, Intestinal Mucosa, Lectins, C-Type, Malondialdehyde, Oligonucleotide Array Sequence Analysis, Oxidants, Oxidative Stress, Pancreatitis-Associated Proteins, Peroxidase, Rats, Superoxide Dismutase, Trinitrobenzenesulfonic Acid, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha.
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Does adenovirus-mediated hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein suppress dextran sulfate sodium-induced acute ulcerative colitis in rats? Context: Although increased expression of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP) has been reported in ulcerative colitis (UC), its role in UC remains unclear. This study was designed to assess the function of HIP/PAP in experimental UC and further to explore its underlying mechanisms. Recombinant adenovirus was prepared to mediate ectopic expression of HIP/PAP in the colon of rats. The effect of HIP/PAP on dextran sodium sulfate (DSS)-induced colitis was assessed by disease activity index (DAI), macroscopic, and histological evaluations. Superoxide dismutase (SOD) and myeloperoxidase (MPO) activities, malondialdehyde (MDA) content, and tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) production were determined in colonic mucosa. Proliferation cell nuclear antigen (PCNA) was immunostained to reflect the proliferation of colonic epithelia. The effects of HIP/PAP on proliferation and H(2)O(2) -induced apoptosis of SW480 and LoVo colonic adenocarcinoma cells were also determined. Gene expression profiles in SW480 after HIP/PAP overexpression were analyzed by microarray analysis. The protective effect of HIP/PAP against DSS-induced colitis in rats was confirmed. Ectopic expression of HIP/PAP resulted in attenuation of oxidative damage, reduction of TNF-α and IL-6 expression, and elevation of epithelial proliferation in colonic mucosa and led to decreased apoptosis and increased proliferation in colonic adenocarcinoma cells. Microarray analysis revealed altered expression of inflammation-related molecules, growth factors, proliferation-related molecules, and antioxidant enzymes under overexpression of HIP/PAP.
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HIP/PAP has a protective effect against DSS-induced colitis in rats via inhibiting inflammation, alleviating oxidative damage, and promoting colonic epithelium regeneration. HIP/PAP might represent a new promising therapeutic strategy in UC. Final Decision: yes