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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Alkaline Phosphatase, Analysis of Variance, Animals, Bone Marrow Cells, Calcification, Physiologic, Cell Differentiation, Cells, Cultured, Collagen, Female, Mesenchymal Stem Cells, Models, Animal, Osteogenesis, Phenotype, Rats, Rats, Wistar, Tetrazolium Salts, Thiazoles, Triiodothyronine.
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Does [ Triiodothyronine increase osteogenic differentiation reduced by age in bone marrow mesenchymal stem cells of female rats ]? Context: To examine if triiodothyronine (T3) increases osteogenic differentiation in bone marrow mesenchymal stem cells (BMMSCs) of adult rats compared with young rats. BMMSCs were cultured in osteogenic medium and distributed into six groups: 1) BMMSCs of young rats; 2) BMMSCs of adult rats; 3, 4, 5 and 6) BMMSCs of adult rats with T3 (0.01, 1, 100 to 1000 nM). We analyzed alkaline phosphatase activity, dimethylthiazol (MTT) conversion, and collagen synthesis at 7, 14, and 21 days, and percentage of cells per field and number of mineralized nodules at 21 days of differentiation. T3 reduced MTT conversion, alkaline phosphatase activity, collagen synthesis, and the synthesis of mineralizalized nodules in at least one of the doses and periods studied (p < 0.05). Values were lower when compared with young and adult rats BMMSCs (p < 0.05) without T3.
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T3 has a negative effect on the factors involved in osteogenic differentiation of BMMSC from adult rats. Final Decision: no