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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, RESULTS. Meshes: Binding Sites, Chromatin Immunoprecipitation, Hep G2 Cells, Humans, Interleukin-1beta, MAP Kinase Signaling System, Macrophages, NF-kappa B, Promoter Regions, Genetic, Ribonucleases, Serum Response Factor, Signal Transduction, Transcription Factors, Transcriptional Activation, ets-Domain Protein Elk-1.
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Are transcription factors Elk-1 and SRF engaged in IL1-dependent regulation of ZC3H12A expression? Context: MCPIP is a novel CCCH zinc finger protein described as an RNase engaged in the regulation of immune responses. The regulation of expression of the gene coding for MCPIP - ZC3H12A is poorly explored. Here we report that the proinflammatory cytokine IL-1beta rapidly induces the synthesis of MCPIP in primary monocyte-derived macrophages and HepG2 cells. This up-regulation takes place through the MAP kinase pathway and following activation of the transcription factor Elk-1. Using a ZC3H12A reporter construct we have shown that a ZC3H12A promoter region, stretching from -76 to +60, mediates activation by IL-1beta. This region contains binding sites for Elk-1 and its partner SRF. Chromatin immunoprecipitation analysis confirms in vivo binding of both transcription factors to this region of the ZC3H12A promoter.
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We conclude that the transcription factor Elk-1 plays an important role in the activation of ZC3H12A expression in response to IL-1beta stimulation. Final Decision: yes