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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS. Meshes: 1,2-Dimethylhydrazine, Administration, Oral, Animals, Apoptosis, Bacillus subtilis, Carcinogenesis, Cell Cycle Checkpoints, Cell Line, Tumor, Clostridium butyricum, Colorectal Neoplasms, Culture Media, Conditioned, Disease Progression, Homeostasis, Inflammation, Male, Mice, Inbred C57BL, Probiotics.
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Do probiotics Clostridium butyricum and Bacillus subtilis ameliorate intestinal tumorigenesis? Context: To investigate the antitumor effects of probiotics Clostridium butyricum and Bacillus subtilis on colorectal cancer (CRC) progression. The effects of C. butyricum and B. subtilis on CRC cells were studied. Male C57BL/6 mice with 1,2-dimethylhydrazine dihydrochloride (DMH)-induced CRC were intervened by these two probiotics and the antitumor effects were examined by comparing the tumor incidence and detecting the inflammatory and immune-related markers.
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C. butyricum and B. subtilis inhibited the proliferation of CRC cells, caused cell cycle arrest and promoted apoptosis. In vivo, these two probiotics inhibited the development of DMH-induced CRC. The molecular mechanism involved reduced inflammation and improved immune homeostasis. This work establishes a basis for the protective role of probiotics B. subtilis and C. butyricum in intestinal tumorigenesis. Final Decision: yes