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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, METHODS, METHODS, METHODS, RESULTS. Meshes: Acetylcarnitine, Annexin A5, Apoptosis, Cell Membrane, Cohort Studies, Cryopreservation, Eosine Yellowish-(YS), Fluorescent Dyes, Humans, Infertility, Male, Male, Outcome Assessment (Health Care), Phosphatidylserines, Progesterone, Prospective Studies, Semen, Semen Preservation, Sperm Motility.
user
Does semen treatment with progesterone and/or acetyl-L-carnitine improve sperm motility or membrane damage after cryopreservation-thawing? Context: To assess the effects of progesterone and acetyl-L-carnitine used before semen cryopreservation-thawing on sperm motility parameters and plasma membrane integrity. Prospective cohort study. Academic tertiary center. Subfertile men undergoing semen evaluation. Before cryopreservation, spermatozoa were incubated with water-soluble progesterone (1 and 10 microM), acetyl-L-carnitine (2.5, 5, 10, and 20 mM), or both (progesterone, 1 microM; and acetyl-L-carnitine, 5 mM). Postthaw change of motility parameters (computer-assisted measurements) and vitality-membrane integrity (examined with eosin-Y staining and annexin V-Cy3 binding assay). There were no statistically significant differences between control samples and samples treated with progesterone and/or acetyl-L-carnitine for cryosurvival rate, motility parameters, or membrane integrity. The percentages of postthaw cells identified as live showed significantly different results with use of the eosin-Y staining and annexin V binding assay.
assistant
Neither progesterone nor acetyl-L-carnitine seemed to prevent cryodamage assessed by motility changes or membrane integrity in human spermatozoa of subfertile men. Annexin V binding, a reflection of membrane translocation of phosphatidylserine, provided more distinct information about postfreezing membrane integrity changes than eosin-Y staining. Final Decision: no