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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: Allergens, Amiloride, Animals, Aspergillus fumigatus, Asthma, Bronchoalveolar Lavage Fluid, Cell Count, Cells, Cultured, Disease Models, Animal, Epithelial Cells, Epithelial Sodium Channels, Interleukin-13, Lung, Mice, Transgenic, Mucociliary Clearance, Pyroglyphidae, STAT6 Transcription Factor, Sodium Channel Blockers.
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Does impaired mucus clearance exacerbate allergen-induced type 2 airway inflammation in juvenile mice? Context: Type 2 airway inflammation plays a central role in the pathogenesis of allergen-induced asthma, but the underlying mechanisms remain poorly understood. We recently demonstrated that reduced mucociliary clearance, a characteristic feature of asthma, produces spontaneous type 2 airway inflammation in juvenile Scnn1b-transgenic (Scnn1b-Tg) mice. To determine the role of impaired mucus clearance in the pathogenesis of allergen-induced type 2 airway inflammation and identify cellular sources of the signature cytokine IL-13. We challenged juvenile Scnn1b-Tg and wild-type mice with Aspergillus fumigatus and house dust mite allergen and compared effects on airway eosinophilia, type 2 cytokine levels, goblet cell metaplasia and airway hyperresponsiveness. Further, we determined cellular sources of IL-13 and effects of genetic deletion of the key type 2 signal transducing molecule STAT6, and evaluated effects of therapeutic improvement of mucus clearance. Reduced mucociliary allergen clearance exacerbated Stat6-dependent secretion of type 2 cytokines, airway eosinophilia and airway hyperresponsiveness in juvenile Scnn1b-Tg mice. IL-13 was elevated in airway epithelial cells, macrophages, type 2 innate lymphoid cells and Th2 cells along with increased Il33 in the airway epithelium of Scnn1b-Tg mice. Treatment with the epithelial Na
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Our data support that impaired clearance of inhaled allergen triggering IL-13 production by multiple cell types in the airways plays an important role in the pathogenesis of type 2 airway inflammation and suggest therapeutic improvement of mucociliary clearance as a novel treatment strategy for children with allergen-induced asthma. Final Decision: yes