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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, METHODS, METHODS, METHODS, RESULTS. Meshes: Animals, Contusions, Insulin-Like Growth Factor I, Male, Muscle, Skeletal, Polymerase Chain Reaction, RNA, Messenger, Rats, Rats, Wistar, Ultrasonic Therapy.
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Does therapeutic ultrasound decrease mechano-growth factor messenger ribonucleic acid expression after muscle contusion injury? Context: To examine the influence of nonthermal ultrasound on mechano-growth factor (MGF) messenger ribonucleic acid (mRNA) expression after blunt trauma. A 2x4 factorial multivariate analysis of variance design. University research laboratory. Thirty-six 3- to 4-month-old male Wistar rats (mean weight, 280.8+/-21.5g). Thirty-two received a bilateral contusion injury to the gastrocnemius via a drop mass technique. Four were control animals. Ultrasound treatment (frequency, 3MHz; intensity, 0.3W/cm(2); continuous duty cycle) was started 24 hours postinjury and delivered for 5 minutes daily on 4 consecutive days. Treatment was on the left hindlimb and the contralateral right hindlimb was the nonultrasound control. Muscle mass (in grams) and MGF mRNA expression as measured via real-time reverse transcriptase polymerase chain reaction. Ultrasound had no effect on muscle mass (F(1,28)=2.723, P=.110, 1-beta=.357, eta(2)=.089). Ultrasound treatments decreased MGF mRNA expression in the treated limb compared with the nontreated hindlimb (F(1,28)=6.605, P=.016, 1-beta=.699, eta(2)=.191).
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The nonthermal ultrasound treatments resulted in decreased MGF mRNA expression after blunt trauma to the gastrocnemius muscles. Final Decision: yes