system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Antineoplastic Agents, Apoptosis, Caspase 3, Cell Nucleus, Cell Survival, Curcumin, Cytochromes c, HT29 Cells, Humans, Inhibitor of Apoptosis Proteins, Membrane Potential, Mitochondrial, Microtubule-Associated Proteins, Mitochondria, Proto-Oncogene Proteins c-bcl-2, RNA, Messenger, Survivin, bcl-2-Associated X Protein, bcl-X Protein.
user
Does curcumin induce apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells? Context: To investigate the effects of curcumin on release of cytochrome c and expressions of Bcl-2, Bax, Bad, Bcl-xL, caspase-3, poly ADP-ribose polymerase (PARP), and survivin of HT-29 cells. HT-29 cells were treated with curcumin (0 approximately 80 micromol/L) for 24 h. The release of cytochrome c from the mitochondria and the apoptosis-related proteins Bax, Bcl-2, Bcl-xL, Bad, caspase-3, PARP, and survivin were determined by Western blot analysis and their mRNA expressions by reverse transcriptase-polymerase chain reaction (RT-PCR). Curcumin significantly induced the growth inhibition and apoptosis of HT-29 cells. A decrease in expressions of Bcl-2, Bcl-xL and survivin was observed after exposure to 10 approximately 80 micromol/L curcumin, while the levels of Bax and Bad increased in the curcumin-treated cells. Curcumin also induced the release of cytochrome c, the activation of caspase-3, and the cleavage of PARP in a dose-dependent manner.
assistant
These data suggest that curcumin induced the HT-29 cell apoptosis possibly via the mitochondria-mediated pathway. Final Decision: yes