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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Animals, Base Sequence, Binding Sites, Cells, Cultured, DNA Primers, Endothelial Cells, Endothelial Growth Factors, Gene Expression Profiling, Humans, Luciferases, Mice, MicroRNAs, Molecular Sequence Data, Promoter Regions, Genetic, Proteins, Proto-Oncogene Protein c-ets-1, Proto-Oncogene Protein c-ets-2, Sequence Deletion, Signal Transduction, Transcriptional Activation.
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Do ets-1 and Ets-2 regulate the expression of microRNA-126 in endothelial cells? Context: MicroRNA plays important roles in vascular biology, but the regulation of endothelial-specific microRNA is not well characterized. MicroRNA-126 (miR-126) is highly expressed in endothelial cells, and it regulates angiogenesis and vascular inflammation. Here we show that the transcription factors Ets-1 and Ets-2 regulate miR-126 expression. A genomic region between -71 and -100 bp upstream of the miR-126 transcriptional start site is critical for transactivation of the gene containing miR-126. This genomic region contains a potential Ets binding site. Mutations within the Ets binding site block transactivation, and Ets-1 and Ets-2 interact with this critical genomic region. Knockdown of endogenous Ets-1 and Ets-2 decreases miR-126 expression. Finally, knockdown of miR-126 alters regulation of an Ets-1 target gene.
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Taken together, these data show that the transcription factors Ets-1 and Ets-2 play a key role in controlling the expression of miR-126 and suggest that miR-126 may mediate some of their vascular effects. Final Decision: yes