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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Active Transport, Cell Nucleus, Anti-Inflammatory Agents, Antioxidants, Aorta, Cell Movement, Cell Survival, Cells, Cultured, Curcumin, Dose-Response Relationship, Drug, Down-Regulation, Humans, Matrix Metalloproteinase 9, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, NF-kappa B p50 Subunit, Reactive Oxygen Species, Transcription Factor RelA, Tumor Necrosis Factor-alpha.
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Does curcumin prevent human aortic smooth muscle cells migration by inhibiting of MMP-9 expression? Context: The migration of vascular smooth muscle cells from the tunica media to the subendothelial region is a key event in the development of atherosclerosis. Curcumin, which is consumed daily by millions of people, is a polyphenol derived from the plant Curcuma longa. In this study, we investigated the effects of curcumin on tumor necrosis factor-alpha (TNF-alpha)-induced cell migration, the formation of intracellular reactive oxygen species (ROS), the translocation of nuclear factor-kappaB (NFkappaB) and the activation and expression of MMP-9 in human aortic smooth muscle cells (HASMCs). The Matrigel migration assay showed that curcumin (10 and 20 micromol/l) effectively inhibited TNF-alpha-induced migration of HASMCs as compared with the control group. To explain this inhibitory effect, MMP-9 was assayed by gelatin zymography and Western blot. The results indicated that curcumin inhibited MMP-9 activity and expression. Furthermore, the production of ROS and the nuclear translocation of NF-kappaB p50 and p65 induced by TNF-alpha were dose-dependently suppressed by curcumin pretreatment.
assistant
These results indicate that curcumin has anti-inflammatory properties and may prevent the migration of HASMCs by suppressing MMP-9 expression through down-regulation of NF-kappaB. Final Decision: yes