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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adaptor Proteins, Signal Transducing, Animals, Cells, Cultured, Eye, Eye Proteins, Female, Fibroblasts, Intracellular Signaling Peptides and Proteins, Male, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Nod1 Signaling Adaptor Protein, Nod2 Signaling Adaptor Protein, RNA, Messenger, Receptors, Cell Surface, Reverse Transcriptase Polymerase Chain Reaction, Toll-Like Receptor 1, Toll-Like Receptor 8, Toll-Like Receptors.
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Does tLRs and nod mRNA expression pattern in healthy mouse eye? Context: To look for TLR and NOD mRNA expression in the healthy eye and in other immune privileged and non-immune privileged mouse organs. Semiquantitative RT-PCR was performed to look for TLR1-9 and NOD1 and NOD2 mRNA expressions in the whole eye, in the anterior (AP) and posterior (PP) portions of the eye, in corneal fibroblasts (CF) and in ovary, brain, testis, heart, lung, and spleen. All the TLR mRNAs were expressed in the whole eye of Balb/c mice. NIH and C57BL/6 did not express TLR9 and TLR8, respectively. NIH expressed higher levels of TLR1, 2, 3, and 6 than the other strains. C57BL/6 expressed the lowest levels of all TLRs. TLR9, 5, and 4 were the less expressed in all strains. All TLRs were expressed in Balb/c PP and TLR1 was not expressed in AP. In NIH and Balb/c CF the majority of TLRs were overexpressed with LPS. In testis, expression of most TLRs was absent. Non-immune privileged organs expressed most of the TLRs. All the organs expressed NOD1 and NOD2. In PP NOD2 was not expressed.
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TLRs and NODs are expressed in the eye, and could have an important role in the innate immunity. Final Decision: yes