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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adult, Anesthetics, Intravenous, Aryldialkylphosphatase, Chromatography, Gel, Dose-Response Relationship, Drug, Electrophoresis, Polyacrylamide Gel, Etomidate, Female, Humans, Ketamine, Kinetics, Propofol.
user
Do intravenous anesthetics inhibit human paraoxonase-1 ( PON1 ) activity in vitro and in vivo? Context: Here we evaluated the in vitro and in vivo effects of the intravenous anesthetics, etomidate, propofol, and ketamine, on the activity of human serum paraoxonase (hPON1). hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. The three anesthetics dose-dependently decreased in vitro hPON1 activity. Inhibition mechanisms are: etomidate was noncompetitive, propofol was competitive, and ketamine was uncompetitive. In vivo studies were performed on five patients for each drug. PON1 was significantly inhibited by 0.3 mg/kg etomidate (p<0.05), 2 mg/kg propofol (p<0.001), and 1 mg/kg ketamine (p<0.05) for up to 5 min following intravenous administration.
assistant
Our results showed that anesthetics significantly inhibit hPON1 activity, both in vitro and in vivo, with rank order etomidate>propofol>ketamine in vitro, and propofol>etomidate>ketamine in vivo. Final Decision: yes