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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Ascorbic Acid, Carrier Proteins, Dopamine, Gene Expression Regulation, Neoplastic, Humans, Neuroblastoma, Norepinephrine, Norepinephrine Plasma Membrane Transport Proteins, Oxidopamine, Symporters, Tumor Cells, Cultured.
user
Does 6-fluorodopamine selectively destroy neuroblastoma cells expressing the noradrenaline transporter? Context: 6-Hydroxydopamine (6-OHDA) was used for ex vivo purging of bone marrow from neuroblastoma cells before autologous transplantation. However, this concept failed because of the rapid autoxidation of 6-OHDA, which leads to the generation of cytotoxic reactive oxygen species (ROS), mainly in the incubation medium before 6-OHDA can be incorporated by neuroblastoma cells. We based our experiments on the theory that, in contrast, 6-fluorodopamine (6-FDA), which is slowly converted to 6-OHDA at neutral pH, is able to enter neuroblastoma cells via the noradrenaline transporter (NA-T). Therefore, most ROS are generated inside the target cells. Small amounts of ascorbate prevent the extracellular conversion of 6-FDA to 6-OHDA without affecting its cytotoxicity, leading to an even more selective effect of 6-FDA.
assistant
We conclude that 6-FDA is a promising substance for selective destruction of NA-T-positive neuroblastoma cells. Final Decision: yes