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Medical AI Inquiry: You were designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.
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In determining protein structure, mercaptoethanol is used to:
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The correct answer is  Ans. C. Reduce disulfide bondsb-mercaptoethanol reduces disulfide bonds. Identification of C-terminus is by Carboxypeptidase C or Y which cleaves any C-terminal amino acid, carboxypeptidase B cleaves only Arg or Lys, carboxypeptidase A does not cleave Arg, Lys, or Pro. Denaturation is disruption of protein structure by adding chemicals or acidic or alkaline reagents, heat etc.