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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, RESULTS. Meshes: CpG Islands, DNA Methylation, DNA-Binding Proteins, Gene Expression Regulation, Humans, Promoter Regions, Genetic, Transcription, Genetic, Transcriptional Activation, Transcriptome.
user
Does integrative analysis of methylome and transcriptome reveal the importance of unmethylated CpGs in non-CpG island gene activation? Context: Promoter methylation is associated with gene repression; however, little is known about its mechanism. It was proposed that the repression of methylated genes is achieved through the recruitment of methyl binding proteins (MBPs) that participate in closing the chromatin. An alternative mechanism suggests that methylation interferes with the binding of either site specific activators or more general activators that bind to the CpG dinucleotide. However, the relative contribution of these two mechanisms to gene repression is not known. Bioinformatics analyses of genome-wide transcriptome and methylome data support the latter hypothesis by demonstrating a strong association between transcription and the number of unmethylated CpGs at the promoter of genes lacking CpG islands.
assistant
Our results suggest that methylation represses gene expression mainly by preventing the binding of CpG binding activators. Final Decision: yes