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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: Adult, Autoantigens, Blotting, Western, Cells, Cultured, DNA-Directed RNA Polymerases, Dose-Response Relationship, Drug, Humans, Male, RNA, Small Cytoplasmic, Reference Values, Ribonucleoproteins, Sensitivity and Specificity, Spleen, Transcription, Genetic.
user
Does ro60 ribonucleoprotein inhibit transcription by T3 RNA polymerase in vitro? Context: Ro60 ribonucleoprotein is a conserved molecule belonging to the family of Ro ribonucleoproteins and targeted by autoantibodies produced in patients with systemic lupus erythematosus or Sjogren's syndrome. Ro60 plays a role in postranscription events, as well as in the nucleocytoplasmic shuttling of RNA polymerase III transcripts. To evaluate the in vitro effects of Ro60 on T3 RNA polymerase transcription. Ro60 ribonucleoprotein was affinity-purified from human spleen extracts using 4B-Sepharose linked to anti-Ro60 monoclonal antibodies. Purified Ro60 was incorporated into the T3 RNA polymerase transcription reaction system using pTRI-beta-Actin-human DNA as a template. Ro60 inhibited initiation of the transcription process in a dose-dependent manner; neither elongation nor termination was affected by Ro.
assistant
In vitro, Ro60 appears to inhibit the transcription of a T3 RNA polymerase-dependent template. Final Decision: yes