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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Animals, Cattle, Cell Movement, Dose-Response Relationship, Drug, Fibroblast Growth Factor 2, In Vitro Techniques, Muscle, Smooth, Vascular, Nitric Oxide.
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Does nitric oxide inhibit the basic fibroblast growth factor-stimulated migration of bovine vascular smooth muscle cells in vitro? Context: The purpose of this study was to evaluate the effect of nitric oxide (NO) on basic fibroblast growth factor (bFGF)-stimulated migration of bovine VSMC in vitro. For the migration assay 50,000 cells from passages 4 to 8 were put into a migration chamber and stimulated with 10 ng bFGF/ml (control group). For another experiment, the NO donator sodium nitroprusside (SNP) (10(-6), 10(-8), 10(-10) M), the second messenger of NO 8-bromo-cGMP (10(-6), 10(-8), 10(-10) M) and 100 U of superoxide dismutase (SOD) were added. Migration of cells was quantified after fixing and staining of the cells by means of light microscopy. Cell migration was significantly inhibited by SNP in a dose dependent manner (p < 0.01 with t-test). This effect was mirrored by 8-bromo-cGMP and not significantly inhibited by SOD, confirming this to be an NO mediated effect and excluding side-effects of superoxide-derived oxidation products.
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Nitric oxide inhibits the basic fibroblast growth factor stimulated migration of bovine vascular smooth muscle cells in vitro. Final Decision: yes