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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Animals, Cells, Cultured, Cryopreservation, Graft Survival, In Vitro Techniques, Interleukin-1, Liver, Liver Transplantation, Male, P-Selectin, Rats, Rats, Inbred Lew, Thrombin, Tissue Distribution, Tumor Necrosis Factor-alpha.
user
Does expression of GMP-140 ( P-selectin ) correlate with graft viability in cold-preserved rat livers? Context: Ischemia/reperfusion injury is an inflammatory process involving cytokine release, Kupffer cell activation, and sinusoidal endothelial cell activation. GMP-140 is synthesized by endothelial cells. We analyzed by Western blotting the expression of GMP-140 in a syngeneic rat liver transplantation model using grafts preserved for different periods of time. Compared with prereperfusion samples, expression did not change significantly in freshly harvested and 4-hr preserved livers. In grafts preserved for 24 hr (100% survival), GMP-140 levels increased dramatically at 1 hr, then returned to baseline at 24 hr after transplantation. Forty-eight hour preserved grafts (0% survival) showed a decreasing expression. To identify possible mediators, the effects of tumor necrosis factor-alpha and interleukin-1beta on GMP-140 expression in primary sinusoidal endothelial cells were analyzed. These cytokines increased both the percentage of stained cells as well as their mean staining fluorescence.
assistant
The absence of increase in 48-hr grafts suggests that GMP-140 may distinguish viable from nonviable livers. Final Decision: yes