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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Becaplermin, Cell Line, Cell Movement, Cytochrome P-450 CYP4A, Drug Synergism, Hydroxyeicosatetraenoic Acids, MAP Kinase Kinase Kinases, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Phosphatidylinositol 3-Kinases, Platelet-Derived Growth Factor, Proto-Oncogene Proteins c-sis, Rats, Signal Transduction.
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Does 20-Hydroxyeicosatetraenoic acid ( 20-HETE ) stimulate migration of vascular smooth muscle cells? Context: We tested the hypothesis that 20-HETE production contributes to platelet derived growth factor (PDGF)-BB stimulated migration of VSMC in a cell culture model. Studies were performed with A10 cells which are a rat vascular smooth muscle derived cell line. Migration was determined using a Boyden chamber chemotactic assay. Pre-treatment of cells with two doses of 20-HETE (100 and 500 nM) significantly increased PDGF-BB stimulated VSMC migration by 34-58% of control; whereas, prior incubation of cells with inhibitors of 20-HETE production, 17-ODYA (1-25 M) or HET0016 (100 nM), significantly decreased PDGF-BB stimulated migration by 40-90%. 20-HETE mediated increase in PDGF-BB migration was completely prevented by the 20-HETE antagonist, WIT-002. In order to determine what second messenger pathways are involved in the 20-HETE mediated stimulation of VSM migration, experiments were performed with specific inhibitors of tyrosine kinase (tyrphostin 25, 10 microM), mitogen-activated extracellular signal-regulated kinase (MEK, PD98059, 20 microM and U0126, 10 microM), protein kinase C (Myr-PKC, 50 microM), and phosphoinositide 3-kinases (PI3Ks) (wortmannin, 50 nM). Blockade of MEK and PI3K all abolished the increase in 20-HETE mediated migration.
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20-HETE stimulates PDGF-mediated VSM migration acting through pathways that involve MEK and PI3K. Final Decision: yes