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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, RESULTS. Meshes: Animals, COS Cells, Cercopithecus aethiops, Cytoplasm, Fluorescent Antibody Technique, HeLa Cells, Humans, In Situ Hybridization, Fluorescence, Inclusion Bodies, Mice, NIH 3T3 Cells, Prions, Protein Folding.
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Do aggresomes represent a general cellular response to protein misfolding in mammalian cells? Context: Aggresomes are juxtanuclear inclusion bodies that have been proposed to represent a general cellular response to misfolded proteins in mammalian cells. Yet, why aggresomes are not a pathological characteristic of protein misfolding diseases is unclear. Here, we investigate if a misfolded protein inevitably forms aggresomes in mammalian cells. We show that a cytoplasmic form of the prion protein may form aggresomes or dispersed aggregates in different cell lines. In contrast to aggresomes, the formation of dispersed aggregates is insensitive to histone deacetylase 6 inhibitors and does not result in cytoskeleton rearrangements. Modulation of expression levels or proteasome inhibitors does not alter the formation of dispersed aggregates.
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Our results establish that aggresomes are not obligatory products of protein misfolding in vivo. Final Decision: no