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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS. Meshes: Age Factors, Animals, Antibodies, Breeding, Dogs, Dose-Response Relationship, Immunologic, Enzyme-Linked Immunosorbent Assay, Female, Immunoglobulin E, Male, Reference Values, Sensitivity and Specificity, Sex Factors.
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Do influence of sex and age on serum total immunoglobulin E concentration in Beagles? Context: To establish an ELISA for detection of serum total IgE concentration in dogs and to analyze IgE values in a dog colony. 147 healthy Beagles (31 males and 116 females). 2 canine IgE-specific polyclonal antibodies elicited by 2 recombinant fragments of the epsilon chain in hens were used to develop a capture ELISA specific for serum total IgE concentration. The IgE values were calculated by comparing serum dose-response curves (1:50 to 1:6,400) with a reference serum pool assigned 100 relative ELISA units (REU). Results-Mean IgE concentration in female Beagles was 51.2 REU (range, 0 to 337.8 REU; median, 31.4 REU), whereas mean IgE concentration in male dogs was only 7.5 REU (range, 0 to 32.6 REU; mean, 3.6 REU). Distribution of IgE values was skewed; approximately 80% of dogs had IgE values < 50 REU. Analysis of natural logarithmically transformed IgE values indicated that sex and age significantly (P < 0.05) influenced IgE values; mean serum IgE values increased until the age of 4 years. Heritability estimates of IgE concentration indicated a trend toward a genetic influence.
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A reliable capture ELISA specific for canine IgE was developed. Serum total IgE values vary with age and sex in the sample population. Final Decision: yes