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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: Acute Disease, Amylases, Animals, Bacterial Translocation, Cecum, Disease Models, Animal, Enterococcus, Enzyme Inhibitors, Escherichia coli, Isothiuronium, Liver, Lymph Nodes, Male, Mesentery, Nitric Oxide Synthase, Nitric Oxide Synthase Type II, Organ Culture Techniques, Pancreas, Pancreatitis, Peritoneum, Rats, Rats, Sprague-Dawley.
user
Does inhibition of inducible nitric oxide synthase reduce bacterial translocation in a rat model of acute pancreatitis? Context: Translocation of bacteria from the gut into pancreatic necrosis is an important factor in the development of septic complications and mortality in acute pancreatitis. S-methylisothiourea (SMT) is an inducible nitric oxide synthase inhibitor that has been shown to decrease bacteria] translocation in sepsis and thermal injury. To investigate whether SMT could affect bacterial translocation in acute necrotizing pancreatitis. Forty-five Sprague-Dawley rats were studied. Acute pancreatitis was induced in Group I and Group II by injection of taurocholate and trypsin into the common biliopancreatic duct. Group III underwent laparotomy with the manipulation (but not cannulation) of the pancreas and received saline injection. Group I rats received normal saline as a placebo, and Group II rats received SMT after surgery for 2 days. At 48 hours, blood was drawn for serum amylase determinations. Bacterial translocation to mesenteric lymph nodes and distant sites (pancreas, liver, and peritoneum) were examined. A point scoring system of histologic features was used to evaluate the severity of pancreatitis. Plasma amylase levels and pancreatic histologic score were significantly reduced in Group II rats given SMT compared with those in Group I rats given saline (p < 0.01, p < 0.05, respectively). All Group I rats had bacterial translocation to mesenteric lymph nodes compared with 7 of 12 rats in Group II (p < 0.05). There was no difference in bacterial translocation to distant organs between the two groups, although rates tended to be lower in Group II compared with Group I (p > 0.05). Bacterial counts in the pancreas were significantly reduced in Group II rats compared with those in Group I rats (p < 0.05).
assistant
Treatment with SMT appears to have ameliorated the course of acute pancreatitis; however, mortality was not affected. Final Decision: yes