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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, METHODS, RESULTS. Meshes: Aggrecans, Animals, Antioxidants, Caspase 3, Cell Survival, Cells, Cultured, Chondrocytes, Collagen Type I, Collagen Type II, Gene Expression Regulation, Hydrogen Peroxide, Nitrites, Oxidative Stress, Proteoglycans, Rats, Rats, Sprague-Dawley, Vitamin E.
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Does vitamin E protect chondrocytes against hydrogen peroxide-induced oxidative stress in vitro? Context: This study evaluated the effect of an antioxidant, Vitamin E, on cultured chondrocytes against H₂O₂-induced damage in vitro. Rat chondrocytes isolated from articular cartilage. Chondrocytes were pretreated with either 50 or 100 μM Vitamin E or serum-free medium for 24 h followed by their exposure to 200 μM H₂O₂ for 3 h. Chondrocytes without exposure to H₂O₂ served as control group. The effect of Vitamin E pretreatment was evaluated by examining proteoglycan contents, nitrite levels, viability, apoptosis, and senescence of cultured chondrocytes. Proteoglycan contents increased in groups treated with Vitamin E. Semi-quantitative real-time PCR data also correlated with these results and demonstrated that Vitamin E up-regulated expression of Agc1, Col2a1, and PCNA genes along with down-regulation in the expression of Col1a1 and Casp3 genes. The differentiation index improved after Vitamin E pretreatment. Nitrite levels were reduced with a corresponding increase in cell viability. Reduction in apoptosis and senescence was also observed after Vitamin E pretreatment. Moreover, a dose-dependent effect of Vitamin E was seen. In contrast to 50 μM Vitamin E, 100 μM was more potent in inducing protection of chondrocytes from H₂O₂-induced oxidative damage.
assistant
Vitamin E reversed the oxidant-induced alterations in chondrocytes and may be a good option to pretreat chondrocytes before transplantation. Final Decision: yes