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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: UNLABELLED. Meshes: Antihypertensive Agents, CD40 Antigens, Cells, Cultured, Endothelin-1, Humans, Interleukin-6, Monocytes, NF-kappa B, Oligopeptides, Peptides, Cyclic, Piperidines, Vasculitis.
user
Does endothelin-1 induce CD40 but not IL-6 in human monocytes via the proinflammatory transcription factor NF-kappaB? Context: The vasoactive peptide endothelin-1 (ET-1) may contribute to the pathogenesis of atherosclerosis and its acute complications. Because inflammation of the vessel wall is a characteristic feature of atherosclerosis, this study investigated the effect of ET-1 on the proinflammatory transcription factor NF-kappaB in monocytes. Monocyte/macrophages are a major source of inflammatory mediators in atheroma and are located in rupture prone plaque areas. In human monocytes ET-1 caused NF-kappaB activation. Specificity of ET-1-induced NF-kappaB activation was ascertained by supershift and competition experiments. This ET-1 effect was blocked by the ET-A-receptor antagonist BQ-123 but not by the ET-B-receptor antagonist BQ-788. PI-1, a specific inhibitor of the IkappaB-alpha-degrading proteasome complex, also prevented NF-kappaB activation. ET-1 stimulated expression of the proinflammatory molecule CD40 but not of the cytokine IL-6 in a NF-kappaB-dependent manner.
assistant
The data demonstrate the ability of ET-1 to activate inflammatory pathways in human monocytes differentially. Final Decision: yes