system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, RESULTS. Meshes: 3' Untranslated Regions, Adult, Antibodies, Base Sequence, Cell Line, Tumor, Cell Movement, Down-Regulation, Female, Helicobacter pylori, Humans, Immunohistochemistry, Male, MicroRNAs, Middle Aged, RNA, Messenger, STAT4 Transcription Factor, Sequence Alignment, Stomach Neoplasms, Up-Regulation.
user
Does down-regulation of miR-141 induced by helicobacter pylori promote the invasion of gastric cancer by targeting STAT4? Context: The association between Helicobacter pylori infection and gastric cancer has been identified recently. However, the molecular mechanism remained largely unknown. We found that miR-141 was decreased in Helicobacter pylori positive specimens (n=75) compared with negative tissues (n=75). The knockdown of miR-141 enhanced the invasion ability of gastric cancer cells; meanwhile, over-expression of miR-141 could inhibit the abilities of gastric cancer cells in vitro. A luciferase assay revealed that miR-141 was directly bound to the 3'-untranslated regions (3'-UTR) of STAT4. STAT4 was found up-regulated at mRNA and protein levels, as shown by qRT-PCR and western blot. Over-expression of STAT4 was used to mimic miR-141 action in the invasion of gastric cancer.
assistant
MiR-141 may play a pivotal role in controlling gastric cancer invasion through regulating STAT4 and maybe a potential target to treat gastric cancer. Final Decision: yes