system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Animals, Apoptosis, Biological Transport, Caspase 3, Caspase 9, Cell Nucleus, Cell Survival, Dexamethasone, Down-Regulation, Enzyme Activation, Glucose, Glucose Transporter Type 3, PC12 Cells, Rats.
user
Does [ Dexamethasone induce PC12 cell apoptosis by down-regulating glucose uptake ]? Context: To observe the effects of dexamethasone (DEX) on rat PC12 chromaffin tumor cells and glucose uptake. PC12 cells in vitro were randomized into normal control group, 10 and 100 μmol/L DEX treated groups. MTT assay was used to determined the cell viability to evaluate the optimal concentration of DEX. Cell apoptosis was measured by DAPI fluorescence staining, mitochondrial membrane permeability transition pore (mPTP), activities of caspase-3 and caspase-9. Glucose oxidase and peroxidase (GOD-POD) assay was performed to detect the glucose consumption. Expression of glucose transporter 3 (GLUT-3) was detected by Western blotting. Compared with the control group, (10, 100) μmol/L DEX significantly decreased cell vitality, caused apoptosis, and reduced the glucose uptake and GLUT-3 protein level (P<0.05).
assistant
DEX can induce the apoptosis of PC12 cells, the mechanism of which may be related to inhibited GLUT-3 protein expression and glucose uptake. Final Decision: yes