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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: UNLABELLED, OBJECTIVE, METHODS. Meshes: Alternative Splicing, Animals, Base Sequence, Bone Marrow, DNA, Complementary, Eosinophils, Exons, Interleukin-5, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Molecular Sequence Data, Protein Isoforms, Receptors, Interleukin, Receptors, Interleukin-5, Reverse Transcriptase Polymerase Chain Reaction, Schistosomiasis mansoni, Solubility, Species Specificity, Transcription, Genetic.
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Does interleukin-5 influence differential transcription of transmembrane and soluble isoforms of IL-5R alpha in vivo? Context: Interleukin-5 (IL-5) promotes signal transduction and expansion of eosinophil colonies in bone marrow via interactions with its heterodimeric receptor (IL-5R). Two variants encoding soluble forms of the alpha subunit (sIL-5R alpha) have been described, although the signals promoting and/or limiting differential transcription remain to be clarified. Our intent was to explore the role of IL-5 in regulating differential transcription of these splice variants in vivo. We have designed a quantitative reverse transcriptase-polymerase chain reaction assay to detect transcripts encoding the transmembrane, soluble 1 and 2 forms of IL-5R alpha in two strains of wild-type (BALB/c and C57BL/6) and corresponding IL-5 gene-deleted mice. Wild-type mice respond to S. mansoni infection with a gradual increase in serum IL-5 and eosinophilia, which is not observed in IL-5 gene-deleted mice.
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We find that IL-5 is not necessary for differential splicing to occur in vivo, as all three forms of the IL-5R alpha are detected in both strains of IL-5 gene-deleted mice, with ratios of transcript expression (transmembrane : soluble 1 : soluble 2) that were indistinguishable from their wild-type counterparts. Differential splicing does vary markedly between strains, potentially because of local effects of strain-specific polymorphisms. Final Decision: no