system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Cells, Cultured, Deoxyglucose, Diabetic Nephropathies, Glomerular Mesangium, Glucose Transporter Type 1, Mice, Mice, Inbred C57BL, Monosaccharide Transport Proteins, RNA, Messenger.
user
Does identification of glucose transporter-1 and it 's functional assay in mouse glomerular mesangial cells cultured in vitro? Context: To evaluate the role of glucose transporter-1 (GLUT1) in the glucose uptake of glomerular mesangial cells. Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was detected by RT-PCR. The expression of GLUT1 protein was detected by immunofluorescence and flow cytometry. The uptake of glucose and its kinetics were determined by 2-deoxy-[3H] -D-glucose uptake. Both GLUT1 mRNA and protein were found in mouse glomerular mesangial cells. 2-deoxy-D-glucose uptake and kinetics assay showed that this glucose transporter had high affinity for glucose and the glucose uptake specificity was further confirmed by phloretin.
assistant
Functional GLUT1 did present in mouse mesangial cells cultured in vitro and it might be the predominant transporter mediated the uptake of glucose into mesangial cells. Final Decision: yes