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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, METHODS, METHODS, RESULTS. Meshes: Adipocytes, Animals, Blood Cell Count, Bone Marrow Cells, Cell Adhesion, Cell Count, Cell Differentiation, Cell Division, Disease Models, Animal, Fibroblasts, Male, Mesenchymal Stem Cells, Osteocytes, Rats, Rats, Sprague-Dawley, Sleep Apnea, Obstructive, Stem Cells.
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Do obstructive apneas induce early release of mesenchymal stem cells into circulating blood? Context: To investigate whether noninvasive application of recurrent airway obstructions induces early release of mesenchymal stem cells into the circulating blood in a rat model of obstructive sleep apnea. Prospective controlled animal study. University laboratory. Twenty male Sprague-Dawley rats (250-300 g). A specially designed nasal mask was applied to the anesthetized rats. Ten rats were subjected to a pattern of recurrent obstructive apneas (60 per hour, lasting 15 seconds each) for 5 hours. Ten anesthetized rats were used as controls. Mesenchymal stem cells from the blood and bone marrow samples were isolated and cultured to count the total number of colony-forming unit fibroblasts (CFU-F) of adherent cells after 9 days in culture. The number of CFU-F from circulating blood was significantly (P = 0.02) higher in the rats subjected to recurrent obstructive apneas (5.00 +/- 1.16; mean +/- SEM) than in controls (1.70 +/- 0.72). No significant (P = 0.54) differences were observed in CFU-F from bone marrow.
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Application of a pattern of airway obstructions similar to those experienced by patients with sleep apnea induced an early mobilization of mesenchymal stem cells into circulating blood. Final Decision: yes