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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Apoptosis, Autophagy, Disulfides, HeLa Cells, Humans, Neoplasms, Phosphofructokinase-2, Piperazines.
user
Does [ PFKFB3 regulate 11'-deoxyverticillin A ( C42 ) -induced autophagy and apoptosis in HeLa cells ]? Context: The aim is to reveal the role of PFKFB3 in 11'-deoxyverticillin A (C42)-induced autophagy and apoptosis. Electron and fluorescence microscopy, immunoblotting, MTS assay, siRNA interference and real time PCR were used. C42 could induce multiple cell death in HeLa cells. Knockdown of either Beclin 1 or LC3, two important autophagic genes, increased both PARP-1 cleavage and cell viability loss. Although high dose of C42 triggered more cell viability loss, yet, it failed to augment autophagic flux. While PFKFB3 inhibitors attenuated C42-induced autophagy, the overexpression of PFKFB3 increased the induced autophagic flux.
assistant
PFKFB3 is involved in C42 induced-autophagy, which blunts the caspase-dependent apoptotic process. Final Decision: yes