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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: ADAMTS5 Protein, Animals, Cattle, Cells, Cultured, Chondrocytes, Dinoprostone, Glycosaminoglycans, Matrix Metalloproteinase 13, Nitric Oxide, Oxygen, Stress, Mechanical, Tumor Necrosis Factor-alpha.
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Does oxygen tension modulate the effects of TNFα in compressed chondrocytes? Context: Oxygen tension and biomechanical signals are factors that regulate inflammatory mechanisms in chondrocytes. We examined whether low oxygen tension influenced the cells response to TNFα and dynamic compression. Chondrocyte/agarose constructs were treated with varying concentrations of TNFα (0.1-100 ng/ml) and cultured at 5 and 21 % oxygen tension for 48 h. In separate experiments, constructs were subjected to dynamic compression (15 %) and treated with TNFα (10 ng/ml) and/or L-NIO (1 mM) at 5 and 21 % oxygen tension using an ex vivo bioreactor for 48 h. Markers for catabolic activity (NO, PGE TNFα dose-dependently increased NO, PGE
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The present findings revealed that TNFα increased production of NO, PGE Final Decision: yes