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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Allergens, Animals, Asthma, Bronchial Provocation Tests, Bronchitis, Bronchoalveolar Lavage Fluid, Dust, Glycoproteins, Humans, Immunoblotting, Immunohistochemistry, Lymphocytes, Mice, Mites, Proteolipids, Pulmonary Surfactant-Associated Protein A, Pulmonary Surfactant-Associated Protein D, Pulmonary Surfactant-Associated Proteins, Pulmonary Surfactants.
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Is allergen-induced bronchial inflammation associated with decreased levels of surfactant proteins A and D in a murine model of asthma? Context: Increasing evidence suggests that pulmonary surfactant protein A (SP-A) and D (SP-D) participate in the lung defence against pathogens. However, the role of surfactant proteins in the pathogenesis of allergen-induced airway inflammation has not been elucidated. In this study we examined the levels and distributions of SP-A and SP-D in a dust mite (Dermatophagoides pteronyssinus, Der p) allergen-induced murine model of asthma. The concentration of SP-A and SP-D in the bronchoalveolar lavage fluid (BALF) and the distribution of surfactant proteins in the lung were assayed by ELISA and immunohistochemistry methods, respectively. The effect of surfactant proteins on allergen-induced pulmonary lymphocyte proliferation was also studied. We demonstrated that there were marked reductions of SP-A and SP-D levels in the BALF of Der p-sensitized BALB/c mice at 48-72 h after allergen challenge (AC). Both purified SP-A and SP-D were able to suppress, in a dose dependent manner, Der p-stimulated intrapulmonary lymphocyte proliferation of naïve mice with saline or allergen challenge, or of Der p-sensitized mice with saline challenge. On the contrary, this suppressive effect was mild (< 9%) on lymphocytes from sensitized mice after AC.
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These results indicated the involvement of pulmonary surfactant proteins in the allergic bronchial inflammation of sensitized mice. Final Decision: yes