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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Antineoplastic Agents, Bystander Effect, Cell Line, Tumor, Cell Survival, Ganciclovir, Genes, Transgenic, Suicide, Genetic Therapy, Humans, Male, Mice, Mice, Nude, Prostatic Neoplasms, Reverse Transcriptase Polymerase Chain Reaction, Simplexvirus, Thymidine Kinase, Tretinoin, Xenograft Model Antitumor Assays.
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Does [ All-trans retinoic acid enhance bystander effect of suicide-gene therapy against androgen-unresponsive prostate cancer ]? Context: To investigate the enhancing effect of all-trans retinoic acid (ATRA) on the bystander effect of the herpes simplex virus thymidine kinase(HSV-TK)/ganciclovir (GCV) against androgen unresponsive prostate cancer. The bystander effect of the HSV-TK/GCV system was measured by methyl thiazolyl tetrazolium (MTT) assay on PC-3 cells before and after ATRA treatment. The growth and the histopathology of transplant tumors were observed in 4 groups of nude mice with prostate cancer. ATRA augmented significantly the bystander effect of the HSV-TK/GCV system by reducing TK positive PC-3 cells from 50% to 30% (P < 0.05). HSV-TK showed an inhibiting effect, while ATRA with the HSV-TK/GCV system produced significant effect on prostate cancer 1 week earlier than the former (P < 0.05).
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ATRA can argument the in vivo and in vitro bystander effect of the HSV-TK/GCV system in the treatment of androgen unresponsive prostate cancer. Final Decision: yes