system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, RESULTS. Meshes: Adaptation, Physiological, Bacterial Proteins, Carrier Proteins, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Gene Knockout Techniques, Hydrogen-Ion Concentration, Lipoproteins, Mutation, RNA, Untranslated, Sigma Factor.
user
Is small noncoding RNA GcvB a novel regulator of acid resistance in Escherichia coli? Context: The low pH environment of the human stomach is lethal for most microorganisms; but not Escherichia coli, which can tolerate extreme acid stress. Acid resistance in E. coli is hierarchically controlled by numerous regulators among which are small noncoding RNAs (sncRNA). In this study, we individually deleted seventy-nine sncRNA genes from the E. coli K12-MG1655 chromosome, and established a single-sncRNA gene knockout library. By systematically screening the sncRNA mutant library, we show that the sncRNA GcvB is a novel regulator of acid resistance in E. coli. We demonstrate that GcvB enhances the ability of E. coli to survive low pH by upregulating the levels of the alternate sigma factor RpoS.
assistant
GcvB positively regulates acid resistance by affecting RpoS expression. These data advance our understanding of the sncRNA regulatory network involved in modulating acid resistance in E. coli. Final Decision: yes