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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Female, HeLa Cells, Humans, Immunoglobulin G, Interleukin-2, Uterine Cervical Neoplasms.
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Does [ Interleukin 2 promote the expression of immunoglobulin G in a cervical cancer cell line HeLaS3 ]? Context: To analyze the effect of interleukin 2 (IL-2) on immunoglobulin G(IgG) expression in cervical cancer cell line HeLaS3. By immunofluorescence-flow cytometry(FCM), we detected membrane and cytoplasmic IgG expression variation of HeLaS3 after IL-2 stimulation. By Western blot, we detected IgG expression variation of HeLaS3 after IL-2 stimulation. Meanwhile, by RT-PCR with primers designed to amplify four subclasses of IgG, we detected Gamma transcript variation in HeLaS3 after IL-2 stimulation at mRNA level. The result of immunofluorescence-FACS showed that after IL-2 stimulation for 48 hours, membrane and cytoplasmic IgG expression elevated in HeLaS3. The result of Western blot showed after IL-2 stimulation for 48 hours, IgG expression elevated in HeLaS3. Meanwhile, the result of RT-PCR showed that Gamma1 transcript mildly elevated after IL-2 stimulation for 48 hours.
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IL-2 can promote IgG expression in cervical cancer cell line HeLaS3. Final Decision: yes