system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Amnion, Case-Control Studies, Chorioamnionitis, Female, Fetal Membranes, Premature Rupture, Galectin 3, Galectins, Humans, Obstetric Labor, Premature, Pregnancy, RNA, Messenger, Real-Time Polymerase Chain Reaction.
user
Is histological chorioamnionitis in pre-term pre-labor rupture of the membranes associated with increased expression of galectin-3 by amniotic epithelium? Context: Gal-3, which can regulate immune responses upon infection and inflammation, was not studied so far in intrauterine infection leading to pre-term pre-labor rupture of the membranes (PPROM), although gal-1 was reported to be implicated in the process. Gal-3 mRNA and protein expression in amnion and its changes during histological chorioamnionitis were studied here. Fetal membranes were obtained from women with PPROM with (n =15) and without histological chorioamnionitis (n =15) during second and third trimester. Immunohistochemical reactivity was evaluated semiquantitatively and analysed using t- test. Galectin profile of amniotic epithelia was determined by PCR and change assessed in gal-3 in PPROM with (n =5) or without histological chorioamnionitis (n =5) by real-time PCR. Human amniotic epithelium was found to express gal-1, -3,-7, and -8 mRNA. Gal-3 mRNA and protein is increased in fetal membranes and in the amniotic epithelium in patients with chorionamnionitis.
assistant
Histological chorioamnionitis is associated with increased gal-3 expression and strong immunoreactivity of the amnion. Gal-3 may participate in the regulation of the inflammatory responses to chorioamniotic infection and/or direct interaction with pathogens. Final Decision: yes