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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Antineoplastic Agents, Phytogenic, Cell Line, Tumor, Female, Humans, MicroRNAs, Paclitaxel, Up-Regulation, Uterine Cervical Neoplasms.
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Does upregulation of microRNA-224 sensitize human cervical cells SiHa to paclitaxel? Context: The purpose of this study was to identify the drug resistant role of miR-224 expression in cervical cancer. Materials and The expression of miR-224 pre- and post-paclitaxel treatment was determined by using stem-loop real-time reverse transcription polymerase chain reaction (RT-PCR). The authors exogenously upregulated miR-224 expression in SiHa cells using miRIDIAN miR-224 mimic transfection and observed its impact on paclitaxel sensitivity using Cytotoxicity assays. MiR-224 was significantly downregulated with fold values at 2.130435 and 4.26087 under five and ten nM paclitaxel treatments, respectively. MiR-224 expression is markedly increased in SiHa cells after transfected with miRIDIAN miR-224 mimic. Exogenous miR-224 facilitates paclitaxel sensitivity in cervical cancer cells. The IC50 value was decreased in SiHa with overexpression of miR-224 compared with miRNA-negative control (p < 0.0001).
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The results suggests that miR-224 might serve as a predictor for paclitaxel response or a therapeutic target in cervical cancer therapy. Final Decision: yes