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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Antineoplastic Agents, Antineoplastic Agents, Phytogenic, Apoptosis, Breast Neoplasms, Caspase 3, Cell Cycle Checkpoints, Cell Line, Tumor, Cell Proliferation, Cladosporium, Cytochromes c, DNA Fragmentation, Down-Regulation, Female, Fungal Proteins, Humans, Mitochondria, NF-kappa B, Paclitaxel, Up-Regulation, bcl-2-Associated X Protein, bcl-X Protein.
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Does fungal taxol extracted from Cladosporium oxysporum induce apoptosis in T47D human breast cancer cell line? Context: The present study concerns molecular mechanisms involved in induction of apoptosis by a fungal taxol extracted from the fungus Cladosporium oxysporum in T47D human breast cancer cells. Apoptosis-induced by the fungal taxol was assessed by MTT assay, nuclear staining, DNA fragmentation, flow cytometry and pro- as well as anti-apoptotic protein expression by Western blotting. Our results showed inhibition of T47D cell proliferation with an IC50 value of 2.5 μM/ml after 24 h incubation. It was suggested that the extract may exert its anti-proliferative effect on human breast cancer cell line by suppressing growth, arresting through the cell cycle, increase in DNA fragmentation as well as down-regulation of the expression of NF-?B, Bcl-2 and Bcl-XL and up-regulation of pro-apoptotic proteins like Bax, cyt-C and caspase-3.
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We propose that the fungal taxol contributes to growth inhibition in the human breast cancer cell through apoptosis induction via a mitochondrial mediated pathway, with possible potential as an anticancer therapeutic agent. Final Decision: yes