system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Alcohol Oxidoreductases, DNA Footprinting, Electrophoretic Mobility Shift Assay, Gene Expression Regulation, Fungal, Glucose, Glycerol, Pichia, Promoter Regions, Genetic, Protein Binding, Real-Time Polymerase Chain Reaction, Repressor Proteins.
user
Is ppNrg1 a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris? Context: The regulator in glycerol repression of Pichia pastoris AOX1 promoter (P AOX1 ) is still unclear. A Cys2His2 zinc finger transcriptional repressor PpNrg1 localized to nucleus and participated in the repression of P AOX1 in P. pastoris in glucose and glycerol. Quantitative real-time PCR revealed that PpNrg1 repressed expression of numerous genes involved in methanol utilization and peroxisome biogenesis in 0.02 % glucose and 1 % (v/v) glycerol. Electrophoretic mobility shift assay and DNase I footprinting assay revealed that PpNrg1 bound to five sites of P AOX1 , including two binding sites of PpMxr1, which is an indispensable activator of P AOX1 in P. pastoris.
assistant
Transcriptional repressor PpNrg1 suppresses P AOX1 in glucose and glycerol by directly binding to five sites of P AOX1 , including two binding sites of transcriptional activator PpMxr1. Final Decision: yes