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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Apoptosis, Caspases, Cell Line, Tumor, Enterovirus A, Human, Humans, Rhabdomyosarcoma, bcl-2-Associated X Protein.
user
Does [ Enterovirus 71 induce apoptosis in a Bax dependent manner ]? Context: To explore the molecular mechanism of apoptosis induced by enterovirus 71 (EV71) infection. The effects of EV71 on Rhabdomyosarcoma (RD) cell viability were detected by CCK8 assay. EV71-induced apoptosis on RD cells were detected by Hoechst 33342 staining and Western blot targeting Caspase 3, 8 and PARP. Bax conformational change was detected by immunoprecipitation with Bax 6A7 antibody. EV71 decreased the viability of RD cells and induces the activation of Caspase 3, 8 and PARP. Bax expression increases in RD cells after EV71 infection, and Bax conformational change also can be detected after EV71 infection.
assistant
Our study reveals that EV71 induces Caspase-dependent apoptosis by Bax conformational change. Final Decision: yes