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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Antioxidants, Apoptosis, Caspase 3, Caspase 9, Catechin, Cytochromes c, Disease Models, Animal, Glutathione, Glutathione Disulfide, Inflammation, Injections, Intraperitoneal, Lipid Peroxidation, Liver, Male, Mice, Mice, Inbred C57BL, Oxidative Stress, Proto-Oncogene Proteins c-bcl-2, Reperfusion Injury, bcl-2-Associated X Protein.
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Does epigallocatechin-3-gallate protect against hepatic ischaemia-reperfusion injury by reducing oxidative stress and apoptotic cell death? Context: To investigate the protective effects of epigallocatechin-3-gallate (EGCG), a major polyphenol source in green tea, against hepatic ischaemia-reperfusion injury in mice. The partial hepatic ischaemia-reperfusion injury model was created by employing the hanging-weight method in C57BL/6 male mice. EGCG (50 mg/kg) was administered via an intraperitoneal injection 45 min before performing the reperfusion. A number of markers of inflammation, oxidative stress, apoptosis and liver injury were measured after the ischaemia-reperfusion injury had been induced. The treatment groups were: sham-operated (Sham, n = 10), hepatic ischaemia-reperfusion injury (IR, n = 10), and EGCG with ischaemia-reperfusion injury (EGCG-treated IR, n = 10). Hepatic ischaemia-reperfusion injury increased the levels of biochemical and histological markers of liver injury, increased the levels of malondialdehyde, reduced the glutathione/oxidized glutathione ratio, increased the levels of oxidative stress and lipid peroxidation markers, decreased B-cell lymphoma 2 levels, and increased the levels of Bax, cytochrome c, cleaved caspase-3, and cleaved caspase-9. Pretreatment with EGCG ameliorated all of these changes.
assistant
The antioxidant and antiapoptotic effects of EGCG protected against hepatic ischaemia-reperfusion injury in mice. Final Decision: yes