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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Animals, Newborn, Apoptosis, Aquaporin 5, Bronchopulmonary Dysplasia, Cell Tracking, Cells, Cultured, Disease Models, Animal, Green Fluorescent Proteins, Hyperoxia, Lung, Mesenchymal Stem Cell Transplantation, Mesenchymal Stem Cells, Peptides, Proto-Oncogene Proteins c-bcl-2, Rats, Rats, Sprague-Dawley, Time Factors, Transfection, Vascular Endothelial Growth Factor A, bcl-2-Associated X Protein.
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Do mesenchymal stem cells protect against neonatal rat hyperoxic lung injury? Context: Bronchopulmonary dysplasia (BPD) is a significant global health problem and currently lacks effective therapy. We established a neonatal rat model of BPD to investigate therapeutic potential of bone marrow-derived mesenchymal stem cells (BMSCs) in neonatal hyperoxic lung injury. BMSCs were isolated, identified, and transfected by lentiviral vector carrying green fluorescent protein gene in vitro. Neonatal Sprague-Dawley rats were injected intravenously with either BMSCs or phosphate-buffered saline following 95% oxygen exposure, and assessed for the survival rate and alveolar injury during recovery. Treatment with BMSCs after oxygen exposure for 7 days improved survival of neonatal rat during recovery. BMSCs protected against neonatal rat hyperoxic lung injury during recovery as demonstrated by enhanced expression of AQP5 and SP-C, likely due to the suppression of alveolar cell apoptosis and lung inflammation responses to oxygen with up-regulation of the expression of BCL-2 gene and down-regulation of the expression of BAX gene and stimulation of vascular endothelial growth factor and so on.
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BMSCs protect against O2-mediated injury partially through stimulation of potent mediators that participate in tissue repair. Final Decision: yes