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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Acid Phosphatase, Adolescent, Animals, Biomechanical Phenomena, Cell Culture Techniques, Cell Differentiation, Chemokine CCL2, Chemokine CXCL1, Cytokines, Dentin, Female, Fibroblasts, Humans, Immunohistochemistry, Interleukin-8, Isoenzymes, Male, Molar, Osteoclasts, Periodontal Ligament, Rats, Rats, Wistar, Receptors, Interleukin-8B, Root Resorption, Stress, Mechanical, Tartrate-Resistant Acid Phosphatase, Tooth Movement Techniques.
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Does iL-8 and MCP-1 induced by excessive orthodontic force mediate odontoclastogenesis in periodontal tissues? Context: The aim of this study was to investigate how interleukin (IL)-8 (cytokine-induced neutrophil chemoattractant; CINC-1) and monocyte chemotactic protein (MCP)-1/CCL2 contribute to root resorption during orthodontic tooth movement. Forty 6-week-old male Wistar rats were subjected to orthodontic force of 10 or 50 g to induce a mesially tipping movement of the upper first molars for 7 days. We determined the expressions of CINC-1, CXCR2, and MCP-1 proteins in root resorption area using immunohistochemistry. Furthermore, we investigated the effects of compression forces (CF) on IL-8 and MCP-1 production by human periodontal ligament (hPDL) cells. We observed an effect of chemokine treatment on rat odonto/osteoclasts in dentin slices that recapitulated root resorption. The immunoreactivity for CINC-1/CXCR2 and MCP-1 was detected in odontoclasts and PDL fibroblasts by the orthodontic force of 50 g on day 7. CF increased the secretion and the expression of mRNA of IL-8 and MCP-1 from PDL cells in a magnitude-dependent manner. Moreover, CINC-1 and MCP-1 stimulated osteoclastogenesis from rat osteoclast precursor cells.
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IL-8 (CINC-1) and MCP-1 may therefore facilitate the process of root resorption because of excessive orthodontic force. Final Decision: yes