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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Cecum, Flow Cytometry, Interleukin-17, Ligation, Mice, Mice, Inbred C57BL, Necrosis, Sepsis, Spleen, T-Lymphocytes, Regulatory, Th17 Cells, Thymus Gland.
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Does [ Transfusion of necrotic cells redress regulatory T cell and Th17 cell imbalance in septic mice ]? Context: To investigate the effect of transfusion of necrotic cells on regulatory T (Treg) and Th17 cell balance in septic mice. Thirty-four C57BL/6 mice were randomized into PBS group (n=5), sham-operated group (n=5), sepsis group (n=12), and necrotic cell transfusion group (n=12) and subjected to intraperitoneal PBS injection, sham operation by separating the cecum only, cecal ligation and puncture (CLP), and injection of 2 × 10⁷ necrotic cells 5 days before CLP, respectively. All the mice were sacrificed 2 weeks after CLP for analyzing the proportion of CD4⁺Foxp3⁺Treg cells and CD4⁺IL17A⁺Th17 cells in the peripheral blood, spleen and thymus by flow cytometry. The percentage of Th17 cells and Treg/Th17 ratio in the spleen was significantly higher in CLP group than in the sham-operated group and PBS group (P<0.01). The percentage of Treg cells in the thymus was significantly lower in CLP group than in the sham-operated group (P<0.01). Pre-infusion of necrotic cells redressed the abnormality of Treg and Th17 cell percentages and Treg/Th17 imbalance in mice following CLP (P<0.05).
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Pre-infusion of necrotic cells can reverse Treg/Th17 imbalance in septic mice. Final Decision: yes