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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adipocytes, Adipogenesis, Adipose Tissue, Cell Differentiation, Humans, MicroRNAs, Obesity, Sp1 Transcription Factor, Stromal Cells, Tumor Necrosis Factor-alpha, Up-Regulation.
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Does microRNA-29b promote the adipogenic differentiation of human adipose tissue-derived stromal cells? Context: Obesity is primarily characterized by the accumulation of large amounts of fat in adipose tissue. Within the adipose tissue, adipocytes are derived from adipose tissue-derived stromal cells (ADSCs) via a specialized cell lineage differentiation process, and ADSCs play a key role in the generation and metabolism of adipose tissue. This study investigated whether microRNAs (miRNAs) play a role in adipocyte differentiation. Using luciferase reporter and ChIP assays, the relationship between miR-29b, SP1, and TNF-α was examined. During the normal adipogenic differentiation of ADSCs, up-regulation of miR-29b promoted adipogenesis by enhancing SP1-mediated inhibition of TNF-α.
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This study investigated the regulatory role of miR-29b during the adipogenic differentiation of ADSCs and found that miR-29b is an effective positive regulator of adipogenesis. Final Decision: yes