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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Base Sequence, Cell Line, Electrophoretic Mobility Shift Assay, HIV Long Terminal Repeat, Heart, Lagerstroemia, Myocardium, NF-kappa B, Plant Extracts, Rats, Tumor Necrosis Factor-alpha.
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Do lagerstroemia speciosa extract inhibit TNF-induced activation of nuclear factor-kappaB in rat cardiomyocyte H9c2 cells? Context: Lagerstroemia speciosa has been used as a folk medicine among people with diabetes in the Philippines. It is known to exhibit antidiabetic, antiobesity, and glucose transport activities through mechanisms not well defined. Diabetes leads to cardiomyocyte hypertrophy in association with an upregulation of vasoactive factors and activation of nuclear factor (NF)-kappaB and activating protein-1. We therefore investigated the effect of Lagerstroemia speciosa on the activation of NF-kappaB as a key mediator of cardiomyocyte hypertrophy, in rat cardiomyocyte H9c2 cells. Water extract of Lagerstroemia speciosa (Lythraceae family) was prepared. H9c2 cells were used for treatment of Lagerstroemia speciosa extract with/without tumor necrosis factor (TNF). To examine NF-kappaB's activation, we performed an electrophoretic mobility shift assay (EMSA). The activation of NF-kappaB by TNF was completely blocked by a Lagerstroemia speciosa extract in a dose- and time-dependent manner in H9c2 cells.
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Overall, our results indicate that Lagerstroemia speciosa can inhibit DNA-binding of NF-kappaB. This may explain its possible inhibition of diabetes-induced cardiomyocyte hypertrophy. Final Decision: yes