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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Actins, Adult, Aged, Aged, 80 and over, Blotting, Western, Cell Line, Tumor, Cell Movement, Female, Gastrectomy, Humans, Lymphatic Metastasis, Male, Middle Aged, Neoplasm Invasiveness, RNA Interference, Stomach Neoplasms, Transfection, Up-Regulation.
user
Is overexpression of β-actin closely associated with metastasis of gastric cancer? Context: β-actin is known to be involved in motility of cancer cells. However, the association between β-actin expression and metastasis of gastric cancer remains unclear. We enrolled 40 gastric cancer patients who underwent gastrectomy and evaluated the expression of β-actin in gastric cancer tissues and the corresponding normal mucosa by the analysis of Western blotting. To further study the function of β-actin in cancer cell migration, wound healing assay was performed in SGC7901 transfected with β-actin siRNA. The level of β-actin was significantly higher in gastric cancer tissues than in the corresponding normal mucosa. The correlation between β-actin expression and the local lymph node metastasis was statistically significant, which demonstrated that overexpression of β-actin might contribute to the local lymph node metastasis of gastric cancer. Interference of β-actin expression could inhibit migration of SGC7901 cells.
assistant
β-actin, the most conservative and dynamic protein, is overexpressed in gastric cancer tissues and may play a crucial role in metastasis of gastric cancer. Final Decision: yes