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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, RESULTS. Meshes: Adaptation, Biological, Bacteria, Catalysis, Kinetics, Models, Genetic, Protein Biosynthesis, RNA, Bacterial, RNA, Messenger, RNA, Transfer, Amino Acid-Specific, Ribosomal Proteins, Ribosomes, Species Specificity, Systems Biology.
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Do a systems approach to model natural variation in reactive properties of bacterial ribosomes? Context: Natural variation in protein output from translation in bacteria and archaea may be an organism-specific property of the ribosome. This paper adopts a systems approach to model the protein output as a measure of specific ribosome reactive properties in a ribosome-mediated translation apparatus. We use the steady-state assumption to define a transition state complex for the ribosome, coupled with mRNA, tRNA, amino acids and reaction factors, as a subsystem that allows a focus on the completed translational output as a measure of specific properties of the ribosome. In analogy to the steady-state reaction of an enzyme complex, we propose a steady-state translation complex for mRNA from any gene, and derive a maximum specific translation activity, Ta(max), as a property of the ribosomal reaction complex. Ta(max) has units of a-protein output per time per a-specific mRNA. A related property of the ribosome, T a(max), has units of a-protein per time per total RNA with the relationshipT a(max) = rho(a)Ta(max), where rho(a) represents the fraction of total RNA committed to translation outpout of P(a) from gene a message. Ta(max) as a ribosome property is analogous to k(cat) for a purified enzyme, and Ta(max) is analogous to enzyme specific activity in a crude extract.
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Analogy to an enzyme reaction complex led us to a ribosome reaction model for measuring specific translation activity of a bacterial ribosome. We propose to use this model to design experimental tests of our hypothesis that specific translation activity is a ribosomal property that is subject to natural variation and natural selection much like Vmax and Km for any specific enzyme. Final Decision: yes