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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Animals, Antibodies, Monoclonal, Aorta, Bronchoalveolar Lavage Fluid, CD18 Antigens, Complement C1 Inactivator Proteins, Complement C1 Inhibitor Protein, Constriction, Creatine Kinase, Female, Kinetics, Leukocyte Count, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Muscle, Skeletal, Reperfusion Injury.
user
Does c1-inhibitor reduce the ischaemia-reperfusion injury of skeletal muscles in mice after aortic cross-clamping? Context: Both C1-inhibitor (C1-INH) and antibodies against the CD18 adhesion molecule have been shown to reduce ischaemia-reperfusion injuries. The objective of this study was to investigate the effect of increased ischaemia times and to determine whether inhibiting C1 or blocking the CD18 function was protective in skeletal muscle ischaemia-reperfusion injury after aortic cross-clamping. BALB/c mice were subjected to aortic cross-clamping below the renal artery for 60, 75 or 105 min, followed by 3 h of reperfusion. Two-thirds of a total dose of anti-CD18 antibody (40 mg/kg) or human C1-INH (1,000 IU/kg) was given by intraperitoneal injection before ischaemia and one-third immediately after the clamping. Creatine kinase (CK) in the plasma was used as an indicator of muscle injury severity. There was a consistent rise in the plasma CK concentration proportional to the length of ischaemia (P < 0.0005). C1-INH treatment significantly (P = 0.012) reduced the plasma CK for the ischaemia times of 75 and 105 min. The anti-CD18 antibody did not have any effect, as demonstrated by the CK values that were similar to controls (P = 0.836).
assistant
The data support a beneficial role for C1-INH in the treatment of ischaemia-reperfusion injuries of skeletal muscles. Final Decision: yes