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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Apoptosis, Carbon Dioxide, Cell Adhesion, Cell Movement, Cell Proliferation, Colonic Neoplasms, Humans, Hypercapnia, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Peritoneal Neoplasms, RNA, Messenger, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Tumor Cells, Cultured.
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Do changes in CO2 concentration increase the invasive ability of colon cancer cells? Context: We studied the effects of CO(2) concentration changes on the invasive ability of colon cancer cells. Colon cancer cell lines and human samples derived from a peritoneal metastasis were incubated in a hypercapnic environment, followed by incubation in 5% CO(2). The invasive ability of colon cancer cells incubated with CO(2) were analyzed using an invasion assay system. In comparison with the colon cancer cell lines incubated in 5% CO(2) only, the invasive ability of cells increased in all the colon cancer cell lines subjected to incubation in 20% CO(2) followed by incubation in 5% CO(2), with a concomitant increase in the mRNA expressions of matrix metalloproteinase-2 (MMP2) and MMP9. The invasive capability of peritoneal metastatic cells in the human-derived specimen also increased on CO(2) concentration changes.
assistant
CO(2) concentration changes enhanced the invasive capacity of colon cancer cells. Final Decision: yes