system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Breast Neoplasms, Cell Adhesion, Cell Line, Tumor, Cell Transformation, Neoplastic, Female, Gene Expression Regulation, Neoplastic, Humans, Lung Neoplasms, Matrix Metalloproteinase 14, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Invasiveness, Neoplasm Metastasis, Protein Kinases, Proto-Oncogene Proteins c-akt, RNA Interference, Repressor Proteins, Transfection.
user
Is aRK5 associated with the invasive and metastatic potential of human breast cancer cells? Context: To investigate the effects of Akt/ARK5 pathways on the metastatic potential of human breast cancer cells. The human ARK5 gene was transfected into MDA-MB-231 cells. Effects of ARK5 on MDA-MB-231 cells were investigated in vitro. The tumorigenicity and spontaneously metastatic capability regulated by ARK5 were determined using an orthotopic xenograft tumor model. ARK5 enhanced the invasive and metastatic potential of MDA-MB-231 cells under regulation by Akt. The enhancement was associated with increasing MMP-2, MMP-9, and MT1-MMP expression. The results were further demonstrated by RNA interference experiment. In an in vivo study, we also demonstrated that ARK5-transfected breast cancer cells grew faster and had more pulmonary metastases than its parental counterparts.
assistant
ARK5 led to a more invasive phenotype and metastatic potential in human breast cancer dependent on Akt. Final Decision: yes