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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Antiprotozoal Agents, Apoptosis, Caspases, Colorimetry, Flow Cytometry, Formazans, Leishmania infantum, Phosphorylcholine, Poly(ADP-ribose) Polymerases, Polymerase Chain Reaction, Tetrazolium Salts.
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Does miltefosine induce metacaspase and PARP genes expression in Leishmania infantum? Context: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase and PARP genes in Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. Anti-leishmania activity of miltefosine was studied by treatment of cultured promastigotes with various concentration of miltefosine. MTT assay and Annexin-V FLUOS staining by using FACS flow cytometry methods were used. Cytotoxic potential of HePC on the amastigots of L.infantum was evaluated in J774 cell line. In addition, metacaspase and PARP genes expression of treated L. infantum were studied. Miltefosine led to dose-dependent death of L. infantum with features compatible with apoptosis. Over expression of metacaspase and PARP was seen 6 hr after treatment.
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Our study showed that miltefosine exerts cytotoxic effect on L. infantum via an apoptotic-related mechanism. Final Decision: yes