system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: 3T3 Cells, Animals, DNA Packaging, Fusion Proteins, gag-pol, Genetic Vectors, HEK293 Cells, HIV-1, Humans, Mice, Transfection.
user
Does genomic integration occur in the packaging cell via unexported lentiviral precursors? Context: To use HIV-1 based lentivirus components to produce gene integration and the formation of a stable cell line in the packaging cell line without viral infection. A co-transfection of a Human Embryonic Kidney (HEK) 293 packaging cell line with Gag-pol (GP) and a transfer vector, without the envelope vector, produces a stable cell line after 2 weeks of selection. Furthermore, a matrix protein deficient GP in the packaging vector enhances this integration. This supports that, in theory, unexported lentiviral cores produced within the packaging cell can infect itself without requiring the release of any lentiviral particles.
assistant
If the packaging cell is also the target cell, then gene integration leading to a stable cell line can be accomplished without viral particle infection. Final Decision: yes