system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: DNA Repair, Humans, Leukocytes, Mononuclear, Mitochondria, Reactive Oxygen Species, Uncoupling Agents.
user
Does inhibition of mitochondrial function reduce DNA repair in human mononuclear cells? Context: Mitochondria provide ATP and Ca(2+) needed for DNA repair, but also produce reactive oxygen species (ROS), which may damage DNA. To investigate the effect of mitochondrial function inhibition on DNA repair. Five mitochondrial inhibitors acting at various sites of electron transport were studied. Human peripheral blood mononuclear cells, spontaneous and H(2)O(2)-induced DNA repair, as well as %-double-stranded-DNA, were measured. All mitochondrial inhibitors suppressed spontaneous and H(2)O(2)-induced DNA repair. However, their effect on %-double-stranded-DNA differed, which is partly related to ROS suppression.
assistant
Mitochondrial inhibition may enhance efficacy and reduce toxicity of radiation and cytotoxic drugs therapy. Final Decision: yes