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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Adult, Cervical Intraepithelial Neoplasia, Down-Regulation, Female, Humans, MicroRNAs, Middle Aged, Papillomavirus Infections, Pilot Projects, Uterine Cervical Neoplasms.
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Is let-7c a Candidate Biomarker for Cervical Intraepithelial Lesions : A Pilot Study? Context: Numerous studies have been performed to discover predictive/prognostic biomarkers for human papillomavirus (HPV) infection and cervical cancer development. More recently, microRNAs were suggested as possible biomarkers of HPV-associated cancers and our aim was to characterize the expression of let-7c in exfoliated cervical cells from women with cervical intraepithelial lesions. Let-7c expression was evaluated by quantitative reverse transcription-polymerase chain reaction in 73 women with normal or cervical intraepithelial lesions: normal epithelium with (n = 17) and without (n = 21) HPV infection; low-grade squamous intraepithelial lesions (n = 14); and high-grade squamous intraepithelial lesions (n = 21). Data showed a trend to down-regulation in women with low-grade squamous intraepithelial lesions (2(-ΔΔCt) = 0.38, p = 0.06) and a significant decreased expression of let-7c in women with low-grade squamous intraepithelial lesions (2(-ΔΔCt) = 0.21; p = 0.004). The combined analysis of all cervical intraepithelial lesions revealed a down-regulation of let-7c expression (2(-ΔΔCt) = 0.27; p = 0.011).
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Our results showed that let-7c expression is significantly changed in the different cervical intraepithelial lesions and its levels should be further investigated as a possible biomarker for cervical intraepithelial lesions using exfoliated cervical cells as the sample source. Final Decision: yes