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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Animals, Burns, Endothelin-1, Male, Neurons, Paraventricular Hypothalamic Nucleus, RNA, Messenger, Rats, Rats, Sprague-Dawley.
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Do [ Effects of burn on synthesis and secretion of endothelin-1 in rat paraventricular nucleus of hypothalamus ]? Context: In order to investigate the pathophysiological role of ET-1 in paraventricular nucleus of hypothalamus (PVH) under burn, changes in synthesis and secretion of endothelin-1 (ET-1) in PVH after burn were observed by in situ hybridization and immunohistochemistry. The intensity of ET-1 mRNA and ET-1-immunoreactivity (ET-1-ir) were quantized by image analysis. Compared with the control group (sham burn, 100% +/- 25%), no significant change in the intensity of ET-1 mRNA positive hybridization signal in PVH was found at 15 min postburn, while the intensity increased significantly both at 60 min (138% +/- 26%, P < 0.05) and at 180 min postburn (167% +/- 18%, P < 0.01). Intensity of ET-1-ir in the neurons in PVH decreased significantly at 15 min postburn to 6.3% +/- 1.5% of the control (P < 0.01) and gradually recovered to 23.1% +/- 2.9% and to 44.1% +/- 3.8% at 60 min and 180 min postburn respectively, while still significantly lower than that in control (P < 0.01).
assistant
The results demonstrated that the synthesis and secretion of ET-1 in rat PVH increased significantly after burn in rats, suggesting that ET-1 in PVH played an important pathophysiological role under burn. Final Decision: yes