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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Aging, Animals, Female, Hypoxia, Mice, Mice, Inbred C57BL, Muscle Fibers, Skeletal, Muscle Proteins, Muscle, Skeletal, Neovascularization, Physiologic, Physical Exertion, Polymerase Chain Reaction, RNA, Messenger, Up-Regulation, Vascular Endothelial Growth Factor A.
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Are soleus , plantaris and gastrocnemius VEGF mRNA responses to hypoxia and exercise preserved in aged compared with young female C57BL/6 mice? Context: In humans, skeletal muscle capillarization and the vascular endothelial growth factor (VEGF) mRNA response to acute exercise are lower in aged compared with young. The exercise-induced increase in VEGF mRNA has been proposed to involve hypoxic regulation of VEGF and is believed to be fibre type-dependent. We hypothesized that attenuated VEGF mRNA responses to hypoxia and exercise with advanced age would be greatest in oxidative vs. glycolytic muscles. 3- and 24-month-old female C57BL/6 mice were exposed to acute hypoxia (FI O2 = 0.06) or performed a single exercise (65% of maximum treadmill running speed) bout. Capillarization and VEGF mRNA were analysed in the soleus, plantaris and gastrocnemius muscles. In each muscle, VEGF mRNA was greater in aged compared with young, while the VEGF mRNA response to acute hypoxia or acute exercise was similar between young and aged. Morphological analysis revealed that type IIA fibre percentage and type IIB capillarization in the plantaris were greater and type IIB fibre cross-sectional area (FCSA) in the gastrocnemius was smaller in aged compared with young.
assistant
These findings suggest that ageing does not impair the potential for non-pathological angiogenesis in mice and that acute exercise increases VEGF mRNA in the soleus, plantaris and gastrocnemius muscles, which differ considerably in fibre type percentage. Final Decision: yes