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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adjuvants, Immunologic, Animals, Antigen-Presenting Cells, Antigens, Basophils, Cell Proliferation, Cells, Cultured, Freund's Adjuvant, Mice, Mice, Inbred BALB C.
user
Do adjuvants determine the contribution of basophils to antigen sensitization in vivo? Context: Basophils expressing FcɛRIα, the alpha chain of the total IgE high affinity receptor, appear to play a role in antigen (Ag) sensitization, although dendritic cells (DCs) are the principal Ag-presenting cells (APCs). To investigate whether these two types of APCs are involved differentially in Ag-sensitization when distinct types of adjuvants are utilized. To investigate whether basophils and DCs serve as APCs in vitro, whole splenocytes, FcɛRIα(+) basophil-depleted splenocytes, and CD11c(+) DC-depleted splenocytes from naïve DO11.10 mice were stimulated in vitro with ovalbumin (OVA) or OVA peptide 323-339 to evaluate Ag-induced proliferation. To investigate whether basophils function as APCs in vivo, BALB/c mice were actively immunized with ragweed (RW) emulsified in alum or Complete Freund's Adjuvant (CFA) followed by an RW challenge in eye drops. An anti-FcɛRIα antibody (Ab) or a control Ab was injected intraperitoneally during the sensitization phase. Twenty-four hours after the RW challenge, conjunctivas and spleens were harvested for histological analysis to evaluate conjunctival eosinophilia and cytokine production, respectively. Depletion of basophils or DCs from naïve DO11.10 splenocytes significantly suppressed proliferative responses to either OVA or OVA peptide. Treatment with the anti-FcɛRIα Ab suppressed the conjunctival eosinophilia when alum, but not CFA, was utilized as the adjuvant. Similarly, the anti-FcɛRIα Ab inhibited cytokine production by splenocytes when alum was used as the adjuvant.
assistant
Adjuvants determine which APCs are utilized in Ag-sensitization. Final Decision: yes