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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Animals, Base Sequence, Cattle, Cell Line, Chromosome Mapping, Chromosomes, Artificial, Bacterial, Cloning, Molecular, DNA, Viral, Dogs, Genome, Viral, Herpesviridae Infections, Herpesvirus 4, Bovine, Herpesvirus 4, Human, Herpesvirus 8, Human, Humans, Molecular Sequence Data, Open Reading Frames, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA.
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Does sequencing of bovine herpesvirus 4 v.test strain reveal important genome features? Context: Bovine herpesvirus 4 (BoHV-4) is a useful model for the human pathogenic gammaherpesviruses Epstein-Barr virus and Kaposi's Sarcoma-associated Herpesvirus. Although genome manipulations of this virus have been greatly facilitated by the cloning of the BoHV-4 V.test strain as a Bacterial Artificial Chromosome (BAC), the lack of a complete genome sequence for this strain limits its experimental use. In this study, we have determined the complete sequence of BoHV-4 V.test strain by a pyrosequencing approach. The long unique coding region (LUR) consists of 108,241 bp encoding at least 79 open reading frames and is flanked by several polyrepetitive DNA units (prDNA). As previously suggested, we showed that the prDNA unit located at the left prDNA-LUR junction (prDNA-G) differs from the other prDNA units (prDNA-inner). Namely, the prDNA-G unit lacks the conserved pac-2 cleavage and packaging signal in its right terminal region. Based on the mechanisms of cleavage and packaging of herpesvirus genomes, this feature implies that only genomes bearing left and right end prDNA units are encapsulated into virions.
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In this study, we have determined the complete genome sequence of the BAC-cloned BoHV-4 V.test strain and identified genome organization features that could be important in other herpesviruses. Final Decision: yes