system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, RESULTS. Meshes: Animals, Blood-Brain Barrier, Body Size, Body Weight, Eating, Female, Gene Expression Regulation, Hypothalamus, In Situ Hybridization, Leptin, Neuropeptides, Obesity, Pregnancy, RNA, Messenger, Rats, Rats, Sprague-Dawley, Receptors, Cell Surface, Receptors, Leptin, Reverse Transcriptase Polymerase Chain Reaction.
user
Does perinatal overfeeding in rats result in increased levels of plasma leptin but unchanged cerebrospinal leptin in adulthood? Context: To study the effect of perinatal programming and overfeeding on the hypothalamic control mechanisms of food intake in adult rats. Neonatal programming effects on body weight, food intake, central and peripheral leptin levels, hypothalamic neuropeptides, leptin receptors and central leptin responsiveness in adult rats. Plasma and cerebrospinal fluid (CSF) leptin levels were analyzed using radioimmunoassay. Neuropeptide mRNA levels were analyzed using in situ hybridization. Leptin receptor mRNA levels were analyzed using reverse transcriptase-polymerase chain reaction. Perinatally overfed rats growing up in small litters (SL) maintain their obese and hyperleptinemic phenotype in adulthood. However, leptin levels in CSF are abnormally low considering the plasmatic hyperleptinemia. In contrast to the already reported changes in perinatally overfed juvenile rats, perinatally overfed adult rats did not show any alteration in the expression of leptin receptor isoforms and evaluated neuropeptides. Moreover, SL adult rats showed a normal sensitivity regarding the inhibitory effect of intracerebroventricular leptin administration on food intake.
assistant
Perinatal overfeeding does not induce alterations in either the anorectic response to central leptin administration or expression of leptin receptors and neuropeptides in adulthood. The leptin resistance to peripheral leptin in SL adult rats may be related to impaired leptin transport across the blood-brain barrier. Final Decision: yes