system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adaptor Proteins, Signal Transducing, Colitis, Ulcerative, Crohn Disease, Gene Expression Profiling, Humans, Ileum, Immunohistochemistry, Inflammatory Bowel Diseases, Paneth Cells, Protein Biosynthesis, Proteins.
user
Does nALP expression in Paneth cells provide a novel track in IBD signaling? Context: Paneth cells are part of the innate mucosal immunity of the gut with possible regulatory function. This study intends to identify the gene expression pattern of the orthotopic and metaplastic Paneth cells, searching for differences between metaplastic occurrence between Crohn's disease and ulcerative colitis. Paneth cells were collected in RNAse-free conditions via micro dissection. RNA isolation and super amplification was followed by microarray analysis of whole genome expression activity of the orthotopic and metaplastic Paneth cells. Immunohistology of beta-catenin and Frizzled-5 receptor was performed. Histological analysis showed no morphological or secretory change (Frizzled-5 receptor and beta-catenin) in orthotopic and metaplastic Paneth cells. Microarray analysis indicated an increased, but not mutant activation of Wnt/beta-catenin signaling and firstly showed expression of NALP 1, 7, 8 and 11 in metaplastic Paneth cells.
assistant
Paneth cells might play a NALP-mediated role in the pathogenesis of IBD. Final Decision: yes