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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Angiogenesis Inducing Agents, Animals, Calcineurin, Calcium-Transporting ATPases, Cell Movement, Cell Proliferation, Cyclooxygenase 2, Disease Models, Animal, Endothelial Cells, HEK293 Cells, Hindlimb, Human Umbilical Vein Endothelial Cells, Humans, Intracellular Signaling Peptides and Proteins, Ischemia, Mice, Mice, Knockout, Muscle Proteins, Muscle, Skeletal, NFATC Transcription Factors, Neovascularization, Physiologic, Plasma Membrane Calcium-Transporting ATPases, RNA Interference, Signal Transduction, Time Factors, Transfection, Vascular Endothelial Growth Factor A.
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Does plasma membrane calcium ATPase isoform 4 inhibit vascular endothelial growth factor-mediated angiogenesis through interaction with calcineurin? Context: Vascular endothelial growth factor (VEGF) has been identified as a crucial regulator of physiological and pathological angiogenesis. Among the intracellular signaling pathways triggered by VEGF, activation of the calcineurin/nuclear factor of activated T cells (NFAT) signaling axis has emerged as a critical mediator of angiogenic processes. We and others previously reported a novel role for the plasma membrane calcium ATPase (PMCA) as an endogenous inhibitor of the calcineurin/NFAT pathway, via interaction with calcineurin, in cardiomyocytes and breast cancer cells. However, the functional significance of the PMCA/calcineurin interaction in endothelial pathophysiology has not been addressed thus far. Using in vitro and in vivo assays, we here demonstrate that the interaction between PMCA4 and calcineurin in VEGF-stimulated endothelial cells leads to downregulation of the calcineurin/NFAT pathway and to a significant reduction in the subsequent expression of the NFAT-dependent, VEGF-activated, proangiogenic genes RCAN1.4 and Cox-2. PMCA4-dependent inhibition of calcineurin signaling translates into a reduction in endothelial cell motility and blood vessel formation that ultimately impairs in vivo angiogenesis by VEGF.
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Given the importance of the calcineurin/NFAT pathway in the regulation of pathological angiogenesis, targeted modulation of PMCA4 functionality might open novel therapeutic avenues to promote or attenuate new vessel formation in diseases that occur with angiogenesis. Final Decision: yes