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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Apolipoprotein A-I, Electrophoresis, Capillary, High-Density Lipoproteins, Pre-beta, Humans, Lecithin Cholesterol Acyltransferase Deficiency, Lipoproteins, HDL, Monitoring, Physiologic, Phosphatidylcholine-Sterol O-Acyltransferase, Phosphatidylcholines.
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Does apoA-I/phosphatidylcholine discs remodel fast-migrating HDL into slow-migrating HDL as characterized by capillary isotachophoresis? Context: Capillary isotachophoresis (cITP) is a technique for characterizing plasma lipoprotein subfractions according to their electrophoretic charges. We used this technique to examine the mechanism by which apoA-I/phosphatidylcholine (POPC) discs increase pre-beta HDL. The cITP analysis was performed using plasma prestained with a lipophilic dye on a Beckman P/ACE MDQ system. Plasma from a patient with lecithin:cholesterol acyltransferase (LCAT) deficiency who had increased apoE-containing HDL was used to characterize the charge distribution of apoA-I/POPC discs. cITP analysis of apoB- and E-depleted plasma of the patient in the presence of apoA-I/POPC discs indicated two major subfractions of apoA-I/POPC discs with mobilities of triglyceride-rich lipoproteins (fast and slow apoA-I). Incubation of whole plasma from a normolipidemic subject in the presence of apoA-I/POPC discs caused a reduction in cITP fast (f)- and intermediate (i)-migrating HDL, and fast and slow apoA-I, and an increase in slow (s)-migrating HDL. The changes in cITP lipoprotein subfractions were not affected by the inhibition of LCAT activity. ApoA-I/POPC discs increased the fractional esterification rate of cholesterol in apoB-depleted plasma.
assistant
ApoA-I/POPC discs remodeled cITP fHDL and iHDL to sHDL independent of LCAT activity. Final Decision: yes