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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Actins, Biflavonoids, Biofilms, Catechin, Enzyme-Linked Immunosorbent Assay, Fibroblasts, Gingiva, Humans, Matrix Metalloproteinase 1, Matrix Metalloproteinase 2, Microscopy, Electron, Scanning, Periodontitis, Porphyromonas gingivalis, Real-Time Polymerase Chain Reaction.
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Do theaflavins inhibit pathogenic properties of P. gingivalis and MMPs production in P. gingivalis-stimulated human gingival fibroblasts? Context: Theaflavins, the main polyphenols in black tea, possesses a wide range of beneficial pharmacological properties. Porphyromonas gingivalis (P. gingivalis) is a major aetiological agent associated with periodontitis, a chronic inflammatory disease affecting tooth-supporting tissues. The aim of the present study is to investigate the effect of theaflavins on pathogenic properties of P. gingivalis and on periodontitis by inhibiting matrix metalloproteinases (MMPs) production induced by this oral pathogen. Microplate dilution assays were performed to determine the effect of theaflavins against planktonic culture and biofilm of P. gingivalis. The effect of theaflavins on gingipain and collagenase activities of P. gingivalis was evaluated using synthetic chromogenic peptides and fluorogenic substrate. Human gingival fibroblasts (HGFs) were stimulated with P. gingivalis in the presence or absence of theaflavins, and then MMP-1, -2 secretion and their mRNA expression were assessed using an enzyme-linked immunosorbent assay (ELISA) and real-time PCR analysis, respectively. Theaflavins exhibited the antimicrobial effects against both planktonic culture and biofilm of P. gingivalis. Theaflavins also markedly inhibited the proteinase activities of P. gingivalis collagenase and gingipains in a dose-dependent manner. Lastly, theaflavins significantly inhibited the secretion and mRNA expression of MMP-1 and MMP-2 by HGFs stimulated with P. gingivalis.
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Theaflavins can affect the virulent properties of P. gingivalis and attenuate the MMP-mediated inflammatory response induced by this pathogen, which suggests that theaflavins may be potentially valuable supplementary therapeutic agent for prevention and treatment of P. gingivalis-associated periodontal diseases. Final Decision: yes