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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Annexin A5, Apoptosis, Breast Neoplasms, Caspase 3, Cell Proliferation, Doxorubicin, Drug Resistance, Neoplasm, Female, Humans, MCF-7 Cells, Membrane Potential, Mitochondrial.
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Are formamidinodoxorubicins more potent than doxorubicin as apoptosis inducers in human breast cancer cells? Context: The ability of five formamidinodoxorubicins to induce apoptosis of MCF-7 breast cancer cells was tested. All these compounds were modified at C-3' and contain a formamidine group (-N=CH-NRR), with the rest of the cyclic secondary amine (HNRR) of a gradually increasing ring size. Cytotoxicity was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. To analyze apoptosis, double staining using fluorescence probes Hoechst 33258/propidium iodide (PI) and annexin V- Fluorescein isothiocyanate/PI was carried-out. Additionally, the TdT-mediated dUTP nick-end labelling test and activity of caspase 3 were determined. The four tested derivatives displayed a significant increase in antiproliferative activity in comparison to doxorubicin. All of the tested derivatives induced caspase-dependent apoptosis of MCF-7 cells.
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DOX-F MOR and DOX-F PAZ analogs are more potent apoptosis inducers than doxorubicin. Final Decision: yes