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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Anti-Bacterial Agents, Antifungal Agents, Candida albicans, Cells, Cultured, Colony Count, Microbial, Dose-Response Relationship, Drug, Hyphae, Microscopy, Electron, Scanning Transmission, Nisin, Time Factors.
user
Does nisin Z inhibit the growth of Candida albicans and its transition from blastospore to hyphal form? Context: To investigate the efficacy of nisin Z, an antimicrobial peptide produced by certain strains of Lactococcus lactis against Candida albicans growth and transition. Candida albicans was cultured in the presence of various concentrations of nisin Z (1000, 500, and 100 microg ml(-1)) for different time points. Candida albicans growth was determined using the Alamar Blue assay. The yeast's transition from blastospore to hyphal form was assessed through optical microscope observations. The effect of nisin Z on C. albicans ultrastructure was followed by scanning and transmission electron microscopy. Our results show that nisin Z inhibited C. albicans growth beginning at 500 microg ml(-1). This inhibition was both time- and dose-dependent. Nisin Z was also active against C. albicans transition by significantly inhibiting the transformation of C. albicans from the blastospore to hyphal form. Treatments with nisin Z lead to ultrastructural disturbances of C. albicans.
assistant
Our findings indicate that nisin Z significantly reduced C. albicans growth and transition. These effects may have occurred through ultrastructural modifications of this yeast. Final Decision: yes