system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Animals, Gene Transfer Techniques, Mice, Neuroblastoma, Plasmids, Tyrosine 3-Monooxygenase, Vaccines, DNA.
user
Is tyrosine hydroxylase-based DNA-vaccination effective against murine neuroblastoma? Context: The disruption of self-tolerance against neuroblastoma is the ultimate goal of an effective DNA-vaccine. Here we demonstrate the induction of a protective immunity against syngeneic murine NXS2 neuroblastoma in A/J mice, following vaccination with tyrosine hydroxylase (TH) derived antigens. Oral gene delivery was accomplished using an attenuated strain of Salmonella typhimurium as a carrier harboring vectors encoding for mTH antigens. Vaccination was effective in protecting animals from a lethal challenge with wild-type NXS2 tumor cells.
assistant
These results provide the first evidence of the TH self antigen being recognized by T-cells and demonstrate that a TH-based DNA vaccine is a potentially useful immunotherapeutic strategy for neuroblastoma. Final Decision: yes