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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adenosine Triphosphatases, Adult, Aged, Animals, Autoantibodies, Autoantigens, Carrier Proteins, Cattle, Cell Cycle Proteins, Electrophoresis, Electrophoresis, Gel, Two-Dimensional, Enzyme-Linked Immunosorbent Assay, Female, Glaucoma, Glaucoma, Open-Angle, Humans, Immunoblotting, Low Tension Glaucoma, Male, Microfilament Proteins, Middle Aged, Optic Nerve, Recombinant Proteins, Valosin Containing Protein.
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Is valosin-containing protein a novel autoantigen in patients with glaucoma? Context: The purpose of this study was to identify novel autoantigens that react with specific serum autoantibodies in patients with glaucoma. Sera from patients with glaucoma (n = 80) and healthy subjects without a known pathology (n = 20) were investigated by immunoblot performed with bovine optic nerve lysates and resolved by one- and two-dimensional electrophoresis. Proteins in the immunoreactive spots were selected and identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) data analysis. All the sera from subjects were assayed using enzyme-linked immunosorbent assay to identify autoantibodies. We selected two prominent bands with molecular weights of 100 and 220 kDa by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, and these two bands were only found in the glaucoma patients. Using one-dimensional electrophoresis and LC-MS/MS analyses, we identified these proteins to be valosin-containing protein (VCP) and fodrin, respectively, and using two-dimensional electrophoresis and LC-MS/MS analyses, VCP was identified to be a common target antigen. In patients with primary open angle glaucoma and normal tension glaucoma, the frequency of autoantibodies to recombinant human VCP was 42.0 and 23.3%, respectively (p < 0.002). In the enzyme-linked immunosorbent assay tests, autoantibody titers to recombinant human VCP were significantly higher than that in healthy controls (p < 0.025).
assistant
VCP represents a potential candidate target for autoantibodies on the optic nerve in patients with glaucoma. Final Decision: yes