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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Caprylates, Cell Membrane, Female, Hippocampus, In Vitro Techniques, Indoles, Male, Maleimides, Membrane Potentials, Protein Kinase C, Protein Transport, Rats, Rats, Wistar, Receptors, Nicotinic, Synaptosomes, Transport Vesicles, Xenopus laevis, alpha7 Nicotinic Acetylcholine Receptor.
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Does linoleic acid derivative DCP-LA stimulate vesicular transport of α7 ACh receptors towards surface membrane? Context: We have earlier found that the linoleic acid derivative DCP-LA could ameliorate learning and memory impairment by targeting α7 ACh receptor. The present study aimed at understanding DCP-LA-regulated α7 ACh receptor trafficking. We monitored currents through α7 ACh receptors expressed in Xenopus oocytes and assayed the receptor mobilizations using fractions with a sucrose density gradient centrifugation, synaptosome preparation, and acutely dissociated neurons from rat hippocampal slices. DCP-LA persistently potentiated α7 ACh receptor currents, and the effect was inhibited by a protein kinase C (PKC) inhibitor or vesicular transport inhibitors. DCP-LA did not induce PKC phosphorylation of α7 ACh receptors. DCP-LA stimulated translocation of α7 ACh receptors from the cytosol toward the plasma membrane or from extra-synaptosomes into synaptosomes and accumulated the receptors at the presynaptic site in hippocampal neurons.
assistant
The results of the present study demonstrate that DCP-LA increases surface localization of α7 ACh receptors in hippocampal neurons, specifically at presynaptic terminals, by stimulating vesicular transport of the receptors, resulting in potentiation of α7 ACh receptor responses, regardless of PKC phosphorylation of the receptors. Final Decision: yes