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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Acetic Acid, Anastomosis, Roux-en-Y, Animals, Blotting, Western, Cell Movement, Dimethyl Sulfoxide, Disease Models, Animal, Flavonoids, Immunohistochemistry, Intestinal Mucosa, Mice, Mitogen-Activated Protein Kinase 6, Mitosis, Pressure, Wound Healing.
user
Does supraphysiologic extracellular pressure inhibit intestinal epithelial wound healing independently of luminal nutrient flow? Context: Luminal pressure may injure the gut mucosa in obstruction, ileus, or inflammatory bowel disease. We formed Roux-en-Y anastomoses in 19 mice, creating proximal and defunctionalized partially obstructed limbs and a distal limb to vary luminal pressure and flow. We induced mucosal ulcers by serosal acetic acid, and assessed proliferation (proliferating cell nuclear antigen) and ERK (immunoblotting). Parallel studies compared Caco-2 enterocyte migration and proliferation after pressure and/or ERK blockade. At 3 days, anastomoses were probe-patent, proximal and distal limbs contained chyme, and defunctionalized limbs were empty. The proximal and defunctionalized limbs showed increased pressure and slower healing despite increased proliferation, ERK protein, and ERK activation. In vitro, pressure decreased Caco-2 migration across collagen or fibronectin, stimulated proliferation, and activated ERK. However, ERK blockade did not prevent pressure effects.
assistant
Luminal pressure during obstruction or ileus may impair mucosal healing independently of luminal flow despite increased mitosis and ERK activation. Final Decision: yes