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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, CD18 Antigens, Cell Adhesion Molecules, Cell Communication, Coloring Agents, Disease Models, Animal, Endothelium, Vascular, Intercellular Adhesion Molecule-1, Leukocytes, Light, Male, Mice, Mice, Inbred C57BL, Muscle, Skeletal, P-Selectin, Stress, Mechanical, Thrombosis, Venules.
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Are mouse cremaster venules predisposed to light/dye-induced thrombosis independent of wall shear rate , CD18 , ICAM-1 , or P-selectin? Context: Microvascular adhesion of platelets to endothelium occurs in response to various inflammatory stimuli, and in venules is often accompanied by adherent leukocytes. In a light/dye injury model, platelet adhesion and thrombi occur preferentially in venules, though the reasons for this predisposition are unknown. The authors sought to determine whether lower wall shear rates or leukocyte-endothelial interactions accounted for preferential platelet thrombi formation in venules relative to arterioles. A light/dye injury model of microvascular thrombosis was used in the mouse cremaster microcirculation. In wild-type mice (n = 17), the time to form microvascular platelet aggregates was delayed in arterioles by 3.1-fold relative to venules (p <.0001). However, arterioles with spontaneously low wall shear rates, as well as arterioles manipulated to reduce wall shear rate to venous levels, still had delayed thrombosis as compared to venules. Similarly, in animals deficient in CD18, P-selectin, or ICAM-1, the time to form platelet thrombi in arterioles was >3.0-fold higher than in venules.
assistant
Mouse cremaster venules are predisposed to light/dye-induced microvascular thrombosis. The data suggest that functional differences between arteriolar and venular endothelial cells (independent of wall shear rate and of CD18, P-selectin, and ICAM-1) account for the venular predisposition to thrombosis. Final Decision: yes