system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine, Acid Phosphatase, Alkaline Phosphatase, Animals, Animals, Newborn, Cell Division, Cells, Cultured, Enzyme Inhibitors, Mice, Mice, Inbred ICR, Osteoblasts, Protein Kinase C, Skull, Tumor Necrosis Factor-alpha.
user
Does protein kinase C inhibitor H-7 block effects of tumor necrosis factor on bone cells? Context: To study the effects of tumor necrosis factor (TNF) on the neonatal mouse osteoblast-enriched calvarial cells and effects of protein kinase C (PK C) inhibitor, 1-(5-isoquinolinesulfonyl)-2 -methylpiperazine (H-7) on the TNF actions. [3H]TdR uptake by the osteoblasts was used to measure cell proliferation. Cellular alkaline phosphatase (AIP) and tartrate resistant acid phosphatase (trAcP) activities were determined spectrophotometrically. TNF (1-100 kU . L-1) inhibited both proliferation and expression of AIP activity, but stimulated trAcP activity. These TNF-induced actions were blocked by simultaneous addition of H-7 (5-20 mumol . L-1).
assistant
TNF has potent effects on the osteoblasts, and the blockade of TNF actions by H-7 suggests that TNF exert its effects through PK C. Final Decision: yes