system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Biological Transport, Caco-2 Cells, Cell Hypoxia, Cytokines, Cytoprotection, Dose-Response Relationship, Drug, Electric Impedance, Epithelial Cells, Gliadin, Glycerophosphates, Humans, Intestinal Absorption, Intestinal Mucosa, Mannitol, Permeability, Time Factors.
user
Does calcium glycerophosphate preserve transepithelial integrity in the Caco-2 model of intestinal transport? Context: To assess the direct effects of ischemia on intestinal epithelial integrity. Furthermore, clinical efforts at mitigating the effect of hypoperfusion on gut permeability have focused on restoring gut vascular function. We report that, in the Caco-2 cell model of transepithelial transport, calcium glycerophosphate (CGP), an inhibitor of intestinal alkaline phosphatase F3, has a significant effect to preserve transepithelial electrical resistance (TEER) and to attenuate increases in mannitol flux rates during hypoxia or cytokine stimulation. The effect was observable even at concentrations as low as 1 μmol/L. As celiac disease is also marked by a loss of gut epithelial integrity, the effect of CGP to attenuate the effect of the α-gliadin peptide 31-55 was also examined. In this instance, CGP exerted little effect of preservation of TEER, but significantly attenuated peptide induced increase in mannitol flux.
assistant
It appears that CGP treatment might synergize with other therapies to preserve gut epithelial integrity. Final Decision: yes