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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Anticoagulants, Cell Adhesion, Cell Movement, Heparin, Low-Molecular-Weight, Leukocyte Count, Leukocytes, Male, Mice, Mice, Inbred BALB C, Microcirculation, Muscle, Skeletal, Regional Blood Flow, Tumor Necrosis Factor-alpha.
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Does low molecular weight heparin inhibit tumor necrosis factor alpha-induced leukocyte rolling? Context: Heparins may exert several anti-inflammatory actions. This study investigated the impact of low molecular weight heparin (LMWH) on tumor necrosis factor (TNF)-alpha-provoked leukocyte rolling, adhesion and extravascular accumulation. Leukocyte-endothelium interactions and tissue accumulation of leukocytes were induced by TNF-alpha in Balb/c mice and analyzed by the use of intravital microscopy in the mouse cremaster muscle microcirculation. We observed that administration of 5000 units/kg of LMWH, but not 50 and 500 units/kg, markedly reduced TNF-alpha-induced leukocyte rolling, adhesion and tissue infiltration. Notably, when LMWH was given after TNF-alpha only leukocyte rolling was reduced and no effect was observed on firm adhesion of leukocytes.
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Taken together, these findings indicate that LMWH inhibits TNF-alpha-induced leukocyte accumulation by inhibiting the rolling adhesive interaction which may help explain the anti-inflammatory effects of LMWH. Final Decision: yes