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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: UNASSIGNED, OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Blotting, Western, Carbon Tetrachloride, Chemical and Drug Induced Liver Injury, Chromatography, High Pressure Liquid, Cytoprotection, GTPase-Activating Proteins, Gene Expression Regulation, Enzymologic, Hyperplasia, Immunohistochemistry, Liver, Liver Cirrhosis, Experimental, Male, Melanthiaceae, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Necrosis, Phosphorylation, Phytotherapy, Plant Extracts, Plants, Medicinal, RNA, Messenger, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Saponins, Signal Transduction.
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Does paridis Rhizoma Sapoinins attenuate liver fibrosis in rats by regulating the expression of RASAL1/ERK1/2 signal pathway? Context: Paridis Rhizoma is a Chinese medicinal herb that has been used in liver disease treatment for thousands of years. Our previous studies found that Paridis Rhizoma saponins (PRS) are the critical components of Paridis Rhizoma which has good liver protection effect. However, the anti-hepatic fibrosis effect and the mechanism of PRS have seldom been reported. To investigate the potential of PRS in the treatment of experimental liver fibrosis and the underlying mechanism. The chemical feature fingerprint of PRS was analyzed by UPLC-PDA. A total of 40 Male Sprague-Dawley (SD) rats were randomly divided into the control group, the model group, the PRS high dose group (PRS H) and the PRS low dose group (PRS L) with 10 rats in each group. The model, PRS H and L groups as liver fibrosis models were established with carbon tetrachloride (CCl Four saponins in PRS were identified from 19 detected chromatographic peaks on UPLC-PDA by comparing to the standard compounds. PRS can improve the degeneration and necrosis of hepatic tissue, reduce the extent of its fibrous hyperplasia according to H&E and MASSON staining detection. As was detected in PRS H and L groups, PRS down-regulated p-ERK1/2 mRNA and RASAL1 protein, and up-regulated the level of p-ERK1/2 mRNA and RASAL1 protein.
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These results demonstrated that PRS can attenuate CCl Final Decision: yes