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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, RESULTS. Meshes: Base Sequence, Carcinoma, Squamous Cell, Cell Cycle, Cell Survival, Gamma Rays, Humans, Laryngeal Neoplasms, Molecular Sequence Data, Oligonucleotides, Antisense, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-raf, Radiation-Sensitizing Agents, Substrate Specificity, Thionucleotides, Tumor Cells, Cultured.
user
Is inhibition of Raf-1 protein kinase by antisense phosphorothioate oligodeoxyribonucleotide associated with sensitization of human laryngeal squamous carcinoma cells to gamma radiation? Context: Previous studies suggest a central role for Raf-1 protein kinase in mitogenic and radiation damage responsive signaling pathways. Here we report that the expression and enzymatic activity of Raf-1 protein are inhibited in SQ-20B cells exposed to raf antisense oligodeoxyribonucleotide (As-ODNs) directed against the translation initiation site of human c-raf-1 cDNA. In contrast, treatment of SQ-20B cells with an equimolar concentration of raf sense oligodeoxy-ribonucleotide (S-ODNs) had no effect on the expression and activity of Raf-1. We have observed radiosensitization of raf As-ODNs-treated SQ-20B cells. The dose modifying factor of As-ODNs treatment was approximately 1.4.
assistant
These studies demonstrate that raf As-ODNs is a DNA sequence-specific radiosensitizer that may have potential for use in the radiation therapy of cancers. Final Decision: yes