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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: METHODS, RESULTS. Meshes: Animals, Cell Movement, Cell Survival, Cells, Cultured, Focal Adhesion Protein-Tyrosine Kinases, Glucose, MAP Kinase Signaling System, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Mice, Mice, Inbred C57BL, Microscopy, Atomic Force, Mitogen-Activated Protein Kinases, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Pseudopodia, RNA, Messenger, Resveratrol, Stilbenes, Wound Healing, src-Family Kinases.
user
Does resveratrol inhibit glucose-induced migration of vascular smooth muscle cells mediated by focal adhesion kinase? Context: Diabetes is a critical factor for atherosclerosis, as hyperglycemia induces vascular smooth muscle cell (VSMC) proliferation and migration and subsequently contributes to the formation of atherosclerotic lesions. This study investigates whether resveratrol plays a regulatory role in the proliferation and migration of VSMCs under high glucose induction to imitate a hyperglycemic condition. Resveratrol inhibited the migration of VSMCs in the wound-healing assay and the formation of lamellipodia and filopodia as assessed by atomic force microscopy scanning. Resveratrol suppressed the mRNA expression of c-Src, Rac1, cdc42, IRS-1, MEKK1, MEKK4, and mitogen-activated protein kinase along with the protein levels of c-Src, p-Src, and cdc42 in VSMCs. Resveratrol decreased the level of p-FAK protein under normal glucose conditions. Resveratrol could inhibit the activities of matrix metalloproteinase (MMP) 2 and MMP 9 as shown by zymography. Moreover, resveratrol also regulated the mitogen-activated protein kinase pathway and MMP activities of VSMC migration under the high glucose condition.
assistant
The antimigratory effects of resveratrol by reduced MMP expression through the inhibition of Rac1, p-FAK, and lamellipodia formation and the activation of p-AKT and p-ERK1/2 suggest that resveratrol is a potential compound for the treatment of vascular diseases via the regulation of VSMC migration. Final Decision: yes