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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Anastomosis, Surgical, Animals, Colon, Disease Models, Animal, Dose-Response Relationship, Radiation, Intestinal Obstruction, Male, Preoperative Care, Rats, Rats, Wistar, Treatment Outcome, Wound Healing.
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Does preoperative irradiation with 5 x 5 Gy in a murine isolated colon loop model cause anastomotic weakening after colon resection? Context: There are conflicting studies on the influence of fractionated preoperative 5 days of 5 Gy irradiation on tissue oxygenation and subsequent colonic anastomotic strength. To elucidate the effect of preoperative irradiation on anastomotic strength, an isolated colon loop model was developed. Male Wistar rats (n = 164) were randomly divided into three groups. One group remained untreated (control). In the other two groups, a loop of descending colon was exteriorized to create a hernia of the abdominal wall. After 4 weeks' recovery, this loop was locally irradiated with 5 x 5 Gy of gamma-rays or sham irradiated. One week after (sham-) irradiation, an anastomosis was performed in all groups. Tissue oxygenation (StO2) was determined with visible light spectroscopy. The animals were sacrificed 3 or 7 days after the operation and the anastomosis was tested for bursting pressure and breaking strength. Irradiated rats showed significantly more weight loss (90% SD 4.3 of initial body weight vs. 96% SD 2.8, p < or = 0.05) and enteritis (18% vs. 5%, p = 0.013) compared to sham and control animals. StO2 was not influenced by irradiation and was not predictive for anastomotic strength. The control group showed significantly lower bursting pressure and breaking strength compared to (sham-) irradiated animals.
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We developed a new isolated loop model for intermittent irradiation of the colon. Preoperative irradiation of the distal part of a colon anastomosis was successfully administered with acceptable side effects and did not cause reduced tissue oxygenation nor clinical signs of anastomotic weakening, nor objective reduction in bursting pressure and breaking strength. Final Decision: no