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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Analysis of Variance, Animals, Blotting, Western, Cell Proliferation, Cells, Cultured, DNA Primers, Dose-Response Relationship, Drug, Drug Evaluation, Preclinical, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Gene Expression Profiling, Glucosides, In Vitro Techniques, Myoblasts, Cardiac, Myocardium, Proliferating Cell Nuclear Antigen, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Sincalide, Stem Cell Factor, Stilbenes, Vascular Endothelial Growth Factor A.
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Is proliferation of rat cardiac stem cells induced by 2 , 3 , 5 , 4'-tetrahydroxystilbene-2-O-β-D-glucoside in vitro? Context: To study the effects of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-d-glucoside (THSG) on proliferation of rat cardiac stem cells (CSCs) in vitro. C-kit(+) cells were isolated from neonatal (1 day old) Sprague-Dawley rats by using flow cytometry. Optimal THSG treatment times and doses for growth of CSCs were analyzed. CSCs were treated with various THSG doses (0, 1, 10, and 100 μM) for 12h. Sorted c-kit(+) cells exhibited self-renewing and clonogenic capabilities. Cell Counting Kit (CCK-8) and Proliferating Cell Nuclear Antigen (PCNA) ELISA test positive cells were significantly increased in THSG-treated groups compared with untreated controls. The percentage of S-phase cells also increased after THSG treatment. Moreover, we show that some c-kit(+) cells spontaneously express vascular endothelial growth factor (VEGF), T-box transcription factor (Tbx5), hyperpolarization-activated cyclic nucleotide-gated 2 (HCN2), hyperpolarization-activated cyclic nucleotide gated 4 (HCN4), alpha myosin heavy chain (αMHC), and beta myosin heavy chain (βMHC) mRNA, and stem cell antigen 1 (Sca-1), cardiac troponin-I, GATA-4, Nkx2.5, and connexin 43 protein were also assessed in CSCs. However, their expression was significantly increased with THSG treatment when compared to untreated controls.
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THSG can increase proliferation of rat CSCs in vitro and thus, shows promise as a potential treatment strategy for stimulating endogenous stem cells to help repair the injured heart after myocardial infarction in patients. Final Decision: yes