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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Anti-Inflammatory Agents, Blotting, Western, Cytokines, Dose-Response Relationship, Drug, Drugs, Chinese Herbal, Enzyme-Linked Immunosorbent Assay, Humans, Interleukin-1beta, Interleukin-4, Leukocytes, Mononuclear, Lipopolysaccharides, Medicine, Korean Traditional, NF-kappa B, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Tumor Necrosis Factor-alpha.
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Does dokhwaljihwang-tang inhibit LPS-induced inflammatory cytokine production in peripheral blood mononuclear cells? Context: Dokhwaljihwang-tang, one of the prescriptions in Four Constitution medicine, has been used to treat neurological disorders. This work was aimed to examine the effect of Dokhwaljihwang-tang on lipopolysaccharide-induced inflammatory cytokine production in peripheral blood mononuclear cells from the lesser yang subjects. The inflammatory cytokines, interleukin 1 beta, interleukin 4 and tumor necrosis factor were measured using the ELISA. The interleukin 1 beta mRNA expression was evaluated with reverse transcriptase-polymerase chain reaction. The expression level of nuclear factor kappa B was examined by western blotting. Dokhwaljihwang-tang significantly inhibited interleukin 1 beta and interleukin 4 production (p<0.05). When Dokhwaljihwang-tang was pre-treated at the dose of 1 mg/ml, it not only decreased the interleukin 1 beta mRNA expression, but also inhibited the activation of nuclear factor kappa B.
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These results suggest the potential of Dokhwaljihwang-tang as a source of pharmaceutical acupuncture for neuronal inflammatory diseases. Final Decision: yes