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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Apoptosis, CDC2 Protein Kinase, Cell Line, Tumor, Cell Proliferation, Coumarins, Cyclin B, Cyclin B1, Cyclin-Dependent Kinases, Drug Screening Assays, Antitumor, G2 Phase, Humans, Lung Neoplasms, Oncogene Protein v-akt, Phosphatidylinositol 3-Kinases, Signal Transduction, bcl-Associated Death Protein.
user
Does osthole induce G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway? Context: To explore the effects of Osthole on the proliferation, cell cycle and apoptosis of human lung cancer A549 cells. Human lung cancer A549 cells were treated with Osthole at different concentrations. Cell proliferation was measured using the MTT assay. Cell cycle was evaluated using DNA flow cytometry analysis. Induction of apoptosis was determined by flow cytometry and fluorescent microscopy. The expressions of Cyclin B1, p-Cdc2, Bcl-2, Bax, t-Akt and p-Akt were evaluated by Western blotting. Osthole inhibited the growth of human lung cancer A549 cells by inducing G2/M arrest and apoptosis. Western blotting demonstrated that Osthole down-regulated the expressions of Cyclin B1, p-Cdc2 and Bcl-2 and up-regulated the expressions of Bax in A549 cells. Inhibition of PI3K/Akt signaling pathway was also observed after treating A549 cells with Osthole.
assistant
Our findings suggest that Osthole may have a therapeutic application in the treatment of human lung cancer. Final Decision: yes