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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Angiotensin II, Angiotensin II Type 1 Receptor Blockers, Animals, Aorta, Thoracic, Carrier Proteins, Cells, Cultured, Chromones, Losartan, Morpholines, Muscle, Smooth, Vascular, Phosphatidylinositol 3-Kinases, Phosphoproteins, Phosphorylation, Rats, Rats, Sprague-Dawley, Ribosomal Protein S6 Kinases, 70-kDa, Signal Transduction.
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Does angiotensin II stimulate phosphorylation of 4E-binding protein 1 and p70 S6 kinase in cultured vascular smooth muscle cells? Context: To examine the regulatory effects of angiotensin II (Ang II) on the phosphorylation of 4E-binding protein 1 (4E-BP1) and p70 S6 kinase in cultured vascular smooth muscle cells (VSMC), and the contribution of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB) signaling pathway in this process. VSMC obtained from rat thoracic aortas were cultured. The phosphorylation of 4E-BP1 and p70 S6 kinase was detected by immunoblotting. Ang II significantly increased the phosphorylation of 4E-BP1 and p70 S6 kinase, with the peaks occurring at, respectively, 10 min and 30 min, after stimulation with Ang II. The stimulatory effect of Ang II on 4E-BP1 and p70 S6 kinase phosphorylation was abrogated by Ang II type 1 receptor (A(T1) receptor) antagonist losartan, and suppressed by PI3K inhibitor LY294002 in a concentration-dependent manner.
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Ang II treatment of VSMC induces the phosphorylation of 4E-BP1 and p70 S6 kinase via A(T1) receptor, and PI3K signaling pathway is involved in this process. Final Decision: yes