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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, RESULTS. Meshes: Adolescent, Adult, Aged, Case-Control Studies, Cells, Cultured, Chloroquine, Cyclosporine, Ethanol, Female, Humans, Immunosuppressive Agents, Irritants, Lithium, Lymphocyte Activation, Male, Middle Aged, Phosphorylation, Phytohemagglutinins, Propranolol, Protein-Tyrosine Kinases, Psoriasis, T-Lymphocytes, Tacrolimus, Time Factors, Tyrosine.
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Is tyrosine phosphorylation in psoriatic T cells modulated by drugs that induce or improve psoriasis? Context: The induction of protein tyrosine kinases (PTKs) is known to be a key element in the activation of lymphocytes. Because immunologic mechanisms are important in the pathogenesis of psoriasis, we examined the time course of tyrosine-phosphorylated proteins (p-tyr) as a marker for cellular PTK activity in phytohemagglutinin (PHA)-stimulated T cells of psoriatic patients and healthy controls. PHA-stimulated T cells from both groups expressed peaks of p-tyr after 15 min and 4 h. In T cells from psoriatics, the 15-min peak was smaller but the 4-hour peak reached an enormous maximum, which was 270% higher than the basic p-tyr value. PHA-stimulated T cells were additionally treated with psoriasis-provoking drugs (lithium, chloroquine, propranolol and ethanol) and the two immunosuppressive drugs cyclosporin A and FK 506. Lithium and propranolol were able to increase the p-tyr level after 15 min in PHA-stimulated T cells from psoriatics in contrast to controls. Chloroquine and ethanol did not have a significant effect on T cells of both groups. CsA markedly diminished the phosphorylation of intracellular tyrosines in T cells of psoriatics and controls, whereas FK 506 diminished the p-tyr level in controls only slightly.
assistant
We have characterized important differences in p-tyr phosphorylation activities of psoriatic T cells compared to controls. This could be a hint to explain the known abnormalities of psoriatic T cells. Final Decision: yes