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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: Alanine Transaminase, Analysis of Variance, Animals, Blood Glucose, Cholesterol, Choline Deficiency, Diet, Dietary Sucrose, Fatty Liver, Fructose, Gene Expression Profiling, Gene Expression Regulation, Lipid Peroxidation, Lipogenesis, Liver, Male, Methionine, Mice, Mice, Mutant Strains, Reverse Transcriptase Polymerase Chain Reaction, Triglycerides.
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Does dietary fructose exacerbate hepatocellular injury when incorporated into a methionine-choline-deficient diet? Context: Methionine-choline-deficient (MCD) diets cause steatohepatitis in rodents and are used to model fatty liver disease in human beings. Recent studies have identified sucrose as a major contributor to MCD-related liver disease through its ability to promote hepatic de novo lipogenesis. To determine whether glucose and fructose, the two constitutents of sucrose, differ in their capacity to provoke steatohepatitis when incorporated individually into MCD formulas. MCD and control formulas prepared with either glucose or fructose as the sole source of carbohydrate were fed to mice for 21 days. Liver injury was assessed biochemically and histologically together with hepatic gene expression and fatty acid analysis. Mice fed MCD formulas developed similar degrees of hepatic steatosis whether they contained glucose or fructose. By contrast, mice fed MCD-fructose developed significantly more hepatocellular injury than mice fed MCD-glucose, judged by histology, apoptosis staining and serum alanine aminotransferase. Liver injury in MCD-fructose mice coincided with an exaggerated rise in the ratio of long-chain saturated to unsaturated fatty acids in the liver. Notably, hepatic inflammation was not enhanced in mice fed MCD-fructose, correlating instead with hepatic lipid peroxidation, which was equivalent in the two MCD groups.
assistant
Fructose is more cytotoxic than glucose when used as the source of carbohydrate in MCD formulas. Final Decision: yes