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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, CD40 Antigens, Dendritic Cells, Female, Galactosylceramides, Immunotherapy, Interferon-gamma, Interleukin-12, Killer Cells, Natural, Lung Neoplasms, Melanoma, Experimental, Mice, Inbred C57BL, Mice, Knockout, Natural Killer T-Cells, Neoplasm Transplantation.
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Does host CD40 be Essential for DCG Treatment Against Metastatic Lung Cancer? Context: For the application of invariant natural killer T (iNKT) cells in cancer therapy, the CD40-CD40L interaction is indispensable in administering alpha-galactosylceramide (αGalCer). We hypothesized that CD40 plays an important role in dendritic cells (DC) pulsed with αGalCer (DCGs) in the treatment of lung metastases. Wild-type (WT) and CD40(-/-) mice were treated with DCGs isolated from WT or CD40(-/-) mice in a B16F10 lung metastases model and NK and NKT cell activity in lungs and the spleen were examined. DCG treatment improved WT mice survival but CD40(-/-) hosts received no survival benefit. Conversely, attenuation of a therapeutic effect in mice treated with CD40(-/-) DCGs was not observed. The functional activities of NK and NKT cells in DCG-treated CD40(-/-) mice were partially suppressed.
assistant
Host CD40 is essential for DCG treatment to have a therapeutic effect on B16F10 lung metastases. Final Decision: yes