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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Amacrine Cells, Animals, Cell Differentiation, Cell Proliferation, Cells, Cultured, DNA Primers, Eye Proteins, Fluorescent Antibody Technique, Indirect, Gene Expression Regulation, Developmental, Gene Transfer Techniques, Homeodomain Proteins, Mice, Mice, Inbred ICR, Neuroglia, PAX6 Transcription Factor, Paired Box Transcription Factors, Plasmids, RNA, Messenger, Repressor Proteins, Retina, Retroviridae, Reverse Transcriptase Polymerase Chain Reaction, SOXB1 Transcription Factors, Stem Cells, Transcriptional Activation.
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Does sox2 play a role in the induction of amacrine and Müller glial cells in mouse retinal progenitor cells? Context: The transcription factor Sox2 plays important roles in both human and mouse retinal development. Although loss-of-function mutations in Sox2 have been studied in mice, gain-of-function experiments in the neural retina have been lacking. The detailed expression pattern of Sox2 in the developing mouse retina was examined by immunohistochemistry. Then, Sox2 was expressed in a retinal explant culture prepared from E17 mouse embryos by retrovirus-mediated gene transfer to examine its role in retinal development. In addition, shRNA was used to suppress Sox2 in a retinal explant using a retrovirus-mediated system. Sox2 was expressed throughout the neuroblastic layer in the embryonic retina, but only in the inner nuclear layer in the mature retina. Double immunostaining revealed that Sox2 was expressed in Müller glial cells and in a subset of amacrine cells. Forced expression of Sox2 in a mouse retinal explant culture resulted in the dramatic accumulation of amacrine cells in the inner nuclear layer; in addition, cells expressing amacrine cell markers were also found on the innermost side of the outer nuclear layer. The expression of Pax6, which plays an important role in amacrine cell differentiation, was observed in the Sox2-expressing cells, and Sox2 activated the Pax6 promoter to drive luciferase expression in Y79 cells. A decrease in retinal progenitor cell proliferation accompanied these effects. The suppression of Sox2 expression by shRNA resulted in a decreased number of cells in the inner nuclear layer.
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Therefore, ectopic Sox2 expression can induce amacrine cells in the mouse retina from stage E17 onward, possibly by facilitating cell cycle exit. Final Decision: yes