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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adenine, Antiviral Agents, Autophagy, Cell Line, Tumor, Enterovirus, Humans.
user
Does [ Autophagy inhibitor 3-MA decrease the production and release of infectious enterovirus 71 particles ]? Context: To determine whether or not enterovirus 71 (enteroviurs 71, EV71) may induce autophagy and affect the production and release of EV71 after the treatment of autophagy inhibitor. Western blots were performed to examine the conversion of LC3-I to LC3- II and the degradation of P62 after the RD-A cells were infected with EV71. CCID50 was determined by checking the virus titer in the supernatant of cells that treated with autophagy inhibitor 3-MA. EV71 infection enhances the type conversion of LC3 and degradation of P62. The infectious virus particles were decreased after the treatment of 3-MA.
assistant
EV71 infection could induce cell autophagy and the autophagy might contribute to the production and release of infectious EV71 particles. Final Decision: yes