system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, METHODS, METHODS, METHODS, RESULTS. Meshes: Cells, Cultured, Endometrium, Female, Humans, Interleukin-6, Phenotype, Polymerase Chain Reaction, Promoter Regions, Genetic, RNA, Messenger, Receptors, Retinoic Acid, Retinoic Acid Receptor alpha, Tretinoin.
user
Does retinoic acid suppress interleukin-6 production in human endometrial cells? Context: To determine whether retinoic acid (RA) can regulate the expression of interleukin (IL)-6 in human endometrial cells in a manner that might be beneficial to women with endometriosis. In vitro study. Academic medical center. Patients with endometriosis and controls. Endometrial cell cultures were treated with RA. Interleukin-6 protein secretion, messenger RNA expression, and IL-6-promoter activity. Using a human endometrial cell line (EM42), as well as primary stromal and epithelial endometrial cells, we demonstrated that RA suppresses IL-6 protein and messenger RNA expression in a time- and dose-dependent fashion, showing maximal effects at pharmacologically achievable blood serum concentrations (micromoles per liter). Retinoic acid specifically inhibited the activity of IL-6-promoter reporter constructs that were transiently transfected into EM42 cells. Mutational analysis of reporter constructs indicated that RA suppression of IL-6 expression was mediated, at least in part, through the nuclear factor IL-6 binding site located in the IL-6 promoter.
assistant
Retinoids may play a fundamental role in altering the pathophysiology of endometriosis related to aberrant production of IL-6. Final Decision: yes