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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Animals, Binding Sites, CHO Cells, Carrier Proteins, Chromatin Immunoprecipitation, Cricetinae, Cricetulus, Down-Regulation, Embryo, Mammalian, Eye, Eye Proteins, Gene Deletion, Gene Expression Regulation, Developmental, Homeodomain Proteins, Immunohistochemistry, In Situ Hybridization, Mice, PAX6 Transcription Factor, Paired Box Transcription Factors, Promoter Regions, Genetic, Protein Binding, Repressor Proteins, Transfection.
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Is cellular retinaldehyde-binding protein ( CRALBP ) a direct downstream target of transcription factor Pax6? Context: Transcription factor Pax6 plays an essential role in the expression of other transcription factors, cell adhesion molecules and is crucial for neurogenesis in the developing forebrain. Analysis of gene expression profiles through microarray experiments in Pax6 mutants allowed us to focus on CRALBP, one of the many genes that were downregulated. We studied the expression of CRALBP in wt and Pax6-/- mutants through in situ hybridization and immunohistochemistry. ChIP assay and luciferase reporter assay were performed to show the regulatory role of Pax6 on CRALBP promoter. RNA and protein expression data show that CRALBP expression was completely abolished in Pax6 mutants. In vivo binding assays and in vitro reporter assays indicate that Pax6 not only binds the promoter of CRALBP but also positively regulates protein expression.
assistant
This work provides evidence supporting that CRALBP is a direct downstream target of Pax6. However, the role of CRALBP in the cortex is yet to be elucidated. Final Decision: yes