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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: 3' Untranslated Regions, Gene Expression, Humans, Intracellular Signaling Peptides and Proteins, MicroRNAs, Neurites, Repressor Proteins, Transfection.
user
Does [ miR-124a promote neurite outgrowth by inhibiting iASPP expression ]? Context: To investigate the role of iASPP as the target gene of miR-124a in neural development. Using the online bioinformatical tool (TargetScan) and by reviewing the relevant studies, we selected iASPP as the candidate target gene of miR-124a involved in early-stage neuronal differentiation. Luciferase reporter assay was used to verify the candidate gene. We transfected M17 cells with a miR-124a overexpression plasmid and detected the changes in the protein expression of iASPP using Western blotting. With retinoic acid-induced M17 cells as the neuronal differentiation model, the role of iASPP in early-stage neuronal differentiation was investigated by gene overexpression and gene interference techniques. miR-124a inhibited the expression of iASPP in M17 cells by interacting with the 3'UTR of iASPP gene. miR-124a promoted neurite outgrowth of the cells, which was blocked by iASPP overexpression.
assistant
miR-124a promotes neurite outgrowth of M17 cells by inhibiting iASPP expression. Final Decision: yes