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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Blood Cell Count, Granulocyte Colony-Stimulating Factor, Lung, Macaca mulatta, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neutropenia, Neutrophils, Pulmonary Circulation.
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Is transient neutropenia after granulocyte-colony stimulating factor administration associated with neutrophil accumulation in pulmonary vasculature? Context: To better define the nature of the transient neutropenia shortly following granulocyte-colony stimulating factor (G-CSF) administration. To evaluate the disappearance of neutrophils, we investigated neutrophil trafficking. Ratios of neutrophil number to background cellularity for C57BL/6 LysM-EGFP knock-in mice and rhesus macaques were determined in the lung, liver, spleen, and kidney after G-CSF administration. For the C57BL/6 LysM-EGFP knock-in mice, the enhanced green fluorescent protein expression (EGFP(+)) cells increased in the lung and spleen within 15 minutes of administering 50 μg/kg G-CSF subcutaneously, and continued to increase in the lung and spleen from 15 minutes to 30 minutes. At 240 minutes, the pulmonary infiltrate declined to a level comparable to the level at 15 minutes, while in the spleen EGFP(+) cells continued to increase. For rhesus macaques, CD18(+) cells also significantly increased in the lung 30 minutes after administration of 10 μg/kg G-CSF subcutaneously compared to the control level.
assistant
These results suggest that the transient neutropenia following G-CSF administration in the mouse and nonhuman primate is associated with an accumulation of neutrophils within pulmonary and splenic vasculature. Final Decision: yes