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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Bone Regeneration, Calcium Phosphates, Cell Culture Techniques, Collagen, Drug Combinations, Endothelium, Vascular, Fibronectins, Flow Cytometry, Laminin, Male, Proteoglycans, Rats, Rats, Inbred Lew, Skull, Stem Cell Transplantation, Stem Cells, X-Ray Microtomography.
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Do peripheral blood-derived endothelial progenitor cells enhance vertical bone formation? Context: This study presents a novel cell-based approach for extra-cortical bone regeneration. To enhance vertical bone formation by combining guided bone regeneration and transplantation of peripheral blood-derived endothelial progenitor cells (EPCs) in a rat calvaria model. EPCs were isolated from peripheral blood of inbred rats. Gold domes (7 mm radius, 5 mm height) were filled with β-tricalcium phosphate (βTCP) mixed with 5 × 10(5) EPC. Domes filled with βTCP served as control (CNT). Rats were sacrificed after 3 months. Vertical bone augmentation was analyzed using histology, histomorphometry, and microcomputed tomography (μCT). In all rats, hard tissue filled the space under the dome. Histomorphometric analysis revealed that EPC transplantations doubled vertical bone height (EPC 4.04 ± 0.22 mm vs CNT 2.29 ± 0.22 mm, p ≤ .001). EPC also caused ∼50% increase in bone area fraction (EPC 47.3 ± 3.1% vs CNT 31.1 ± 2.7%, p ≤ .003). μCT results also showed that bone volume fraction (BV/TV) was higher in EPC group (p = .0169). In both groups, BV/TV declined from the bottom to the top of the samples. No differences in tissue mineral density were found between EPC and CNT groups.
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EPC transplantation significantly improved bone formation especially in the areas that are remote from the original bone. Final Decision: yes