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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Actins, Administration, Oral, Amides, Animals, Cell Division, Cell Movement, Cells, Cultured, Enzyme Inhibitors, Fibroblasts, Fibrosis, Gene Expression, Intracellular Signaling Peptides and Proteins, Kidney, Macrophages, Mice, Nephritis, Interstitial, Protein-Serine-Threonine Kinases, Pyridines, RNA, Messenger, Signal Transduction, Ureteral Obstruction, ras Proteins, rho GTP-Binding Proteins, rho-Associated Kinases, rhoA GTP-Binding Protein, rhoB GTP-Binding Protein, rhoC GTP-Binding Protein.
user
Does y-27632 prevent tubulointerstitial fibrosis in mouse kidneys with unilateral ureteral obstruction? Context: The small GTPase Rho is involved in cell-to-substratum adhesion and cell contraction. These actions of Rho mediated by downstream Rho effectors such as Rho-associated coiled-coil forming protein kinase (ROCK) may be partly responsible for the progression of renal interstitial fibrosis. The anti-fibrosis effects of Y-27632, a specific ROCK inhibitor, were studied both in vivo (unilateral ureteral obstruction; UUO) and in vitro. To investigate the therapeutic efficacy of Y-27632 in UUO kidneys, smooth muscle alpha actin (SMalphaA) expression, macrophage infiltration and fibrosis in the obstructed kidneys were studied. SMalphaA, transforming growth factor beta (TGF-beta), alpha1 (I) collagen, osteopontin, macrophage chemoattractant peptide-1 (MCP-1), and intercellular adhesion molecule-1 (ICAM-1) gene expression were examined by Northern blotting. To elucidate the mechanism linking the Rho-ROCK pathway with renal fibrosis, the effects of Y-27632 on in vitro cell proliferation and cell migration were studied. In vivo analysis showed that Y-27632 suppressed SMalphaA expression, macrophage infiltration and interstitial fibrosis, and that Y-27632 suppressed SMalphaA, TGF-beta and alpha1 (I) collagen mRNA expression. In vitro analysis showed that Y-27632 did not suppress proliferation of renal fibroblasts but suppressed migration of macrophages.
assistant
The Rho-ROCK system may play an important role in the development of tissue fibrosis, and the Rho-ROCK signaling pathway may be a new therapeutic target for preventing interstitial fibrosis in progressive renal disease. Final Decision: yes