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Medical AI Inquiry: You were designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.
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In negative staining-
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The correct answer is  Negative staining : India ink or Nigrosin are emulsified with the simple or organism to provide a uniformly colored background against which the unstained bacteria stand out in contrast. this is paicularly useful in the demonstration of bacteria capsules which do not take simple stains. Very slender bacteria such as spirochetes that are not demonstrable by simple staining methods can be viewed by negative staining Ref :Ananthanarayan & paniker's Textbook of Microbiology9th edition pg no 12