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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Adult, Aged, Aged, 80 and over, Aldehydes, Biomarkers, Blood Platelets, Case-Control Studies, Cysteine, Female, Glutathione, Hepatitis C, Chronic, Humans, Interleukin-6, Male, Malondialdehyde, Middle Aged, Nitric Oxide, Nitrites, Platelet Aggregation, Platelet Aggregation Inhibitors, Platelet Count, Platelet Function Tests, S-Nitrosoglutathione, S-Nitrosothiols, Thrombocytopenia, Tumor Necrosis Factor-alpha.
user
Is platelet aggregation affected by nitrosothiols in patients with chronic hepatitis : in vivo and in vitro studies? Context: To investigate the relationship among the number of platelets and plasma levels of S-nitrosothiols (S-NO), nitrite, total non-protein SH (NPSH), glutathione (GSH), cysteine (CYS), malondialdehyde (MDA), 4-hydroxininenal (4HNE), tumor necrosis factor-alpha (TNFalpha) and interleukin (IL)-6 in patients with chronic hepatitis C (CH). In vitro the aggregation of platelets derived from controls and CH patients was evaluated before and after the addition of adenosine diphosphate (ADP) and collagen, both in basal conditions and after incubation with nitrosoglutathione (GSNO). In vivo, S-NO plasma levels increased significantly in CH patients and they were significantly directly correlated with platelet numbers. Patients with platelet counts < 150000/microL, had a smaller increase in S-NO, lower levels of GSH, CYS, NPSH, TNFalpha, and IL-6, and higher levels of nitrite, MDA, and 4-HNE relative to those of patients with platelet counts > 150000/microL. In vitro, the ADP and collagen aggregation time was increased in platelets from patients and not from controls; in addition, platelets from CH patients but not from controls also showed a latency time after exposure to collagen.
assistant
The incubation of platelets with GSNO improved the percentage aggregation and abolished the latency time. Final Decision: yes