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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Ankyrin Repeat, Breast Neoplasms, Cell Line, Tumor, Cell Proliferation, Female, Gene Knockdown Techniques, Humans, Neoplasm Proteins, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Rats, Rats, Sprague-Dawley, Receptors, Notch, Transcriptome, Up-Regulation.
user
Is overexpression of NOTCH-regulated ankyrin repeat protein associated with breast cancer cell proliferation? Context: NOTCH-regulated ankyrin repeat protein (NRARP) has been implicated in crosstalk between NOTCH and wingless-type mouse mammary tumor virus integration site (WNT) signals during development. Our study aimed to clarify its role in breast cancer cells. Public microarray data were used to analyze gene expression in human and rat breast cancer. A short interfering RNA was introduced into MCF7 and T47D human breast cancer cells for NRARP silencing. Gene expression was analyzed by quantitative polymerase chain reaction. The NRARP transcript was commonly overexpressed in various rat mammary cancer models. In addition, a subset of human breast cancer also expressed high levels of NRARP transcript, which correlated positively with up-regulation of cell proliferation-related genes. Silencing of NRARP suppressed the growth of MCF7 and T47D cells and lowered the expression of cell cycle-related genes in MCF7 cells.
assistant
NRARP may stimulate cell proliferation in human breast cancer. Final Decision: yes