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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Anthracenes, Autophagy, Carcinoma, Cell Line, Tumor, Ceramides, Enzyme Activation, Gene Expression Regulation, Neoplastic, Humans, JNK Mitogen-Activated Protein Kinases, Microtubule-Associated Proteins, Nasopharyngeal Carcinoma, Nasopharyngeal Neoplasms, RNA, Small Interfering, Reactive Oxygen Species, Signal Transduction, Transcription, Genetic, Up-Regulation.
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Is c-Jun NH2-terminal kinase activation essential for up-regulation of LC3 during ceramide-induced autophagy in human nasopharyngeal carcinoma cells? Context: Autophagy is a dynamic catabolic process characterized by the formation of double membrane vacuoles termed autophagosomes. LC3, a homologue of yeast Atg8, takes part in autophagosome formation, but the exact regulation mechanism of LC3 still needs to be elucidated. Ceramide-induced autophagy was determined by detecting LC3 expression with Western blotting and confocal microscopy in human nasopharyngeal carcinoma cell lines CNE2 and SUNE1. The activation of JNK pathway was assessed by Western blotting for phospho-specific forms of JNK and c-Jun. The JNK activity specific inhibitor, SP600125, and siRNA directed against JNK were used to block JNK/c-Jun pathway. ChIP and luciferase reporter analysis were applied to determine whether c-Jun was involved in the regulation of LC3 transcription. Ceramide-treated cells exhibited the characteristics of autophagy and JNK pathway activation. Inhibition of JNK pathway could block the ceramide-induced autophagy and the up-regulation of LC3 expression. Transcription factor c-Jun was involved in LC3 transcription regulation in response to ceramide treatment.
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Ceramide could induce autophagy in human nasopharyngeal carcinoma cells, and activation of JNK pathway was involved in ceramide-induced autophagy and LC3 expression. Final Decision: yes