system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Aniline Compounds, Bone Marrow, Bone Marrow Cells, Calcium, Cells, Cultured, Osteoclasts, Stress, Mechanical, Xanthenes.
user
Does [ Fluid shear stress increase the Ca2+ concentration in bone-marrow derived osteoclast-like cells ]? Context: To study the change of Ca2+ density in cultured osteoclast-like cells in response to fluid shear stress. Laser scanning confocal microscope and fluorescent probe were used to detect the free Ca2+ in osteoclast-like cells before and after undergoing fluid shear stress. The images were analyzed and compared with image software. At 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM. Compared with contol group, the average intensity of Ca2+ fluorescent signal in osteoclast-like cells undergoing fluid shear stress increased significantly.
assistant
The Cal2+ concentration in bone-marrow derived osteoclast-like cells is sensitive to fluid shear stress, which suggests osteoclast-like cells modulate their function in response to fluid shear stress through the change of free Ca2+ concentration. Final Decision: yes