system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Administration, Inhalation, Adrenal Cortex Hormones, Anti-Asthmatic Agents, Asthma, Bronchi, Case-Control Studies, Humans, Mast Cells, Muscle, Smooth, Respiratory Mucosa.
user
Does corticosteroid treatment selectively decrease mast cells in the smooth muscle and epithelium of asthmatic bronchi? Context: Mast cells are important in the pathophysiology of airway inflammation and evidence suggests their sub-localisation within the airway is altered in asthma. Little is known about the effect of corticosteroids on mast cell localisation within the bronchi. We therefore performed an immunohistochemical analysis of mast cell numbers within the smooth muscle, epithelium and submucosa of healthy subjects (n = 10) and well-characterised asthmatic patients, using either β(2)-agonists alone (n = 10) or β(2)-agonists and inhaled corticosteroids (n = 10). Patients using inhaled corticosteroids displayed significantly lower numbers of mast cells within their epithelium and smooth muscle compared to those not treated with inhaled corticosteroids. Submucosal mast cells were not affected by corticosteroid treatment. Numbers of smooth muscle mast cells correlated with bronchial responsiveness and epithelial mast cells with exhaled NO.
assistant
We demonstrate that glucocorticosteroids differentially affect mast cell numbers within specific airway sub-locations highlighting the importance of mast cell and smooth muscle/epithelial interactions in asthma pathogenesis. Final Decision: yes