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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, RESULTS. Meshes: Animals, Antineoplastic Agents, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Line, Tumor, Cell Proliferation, Cytokines, Enterotoxins, Female, Flow Cytometry, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Neoplasms, Real-Time Polymerase Chain Reaction, Receptors, Antigen, T-Cell, alpha-beta, Recombinant Proteins, Superantigens.
user
Does increased T-cell stimulating activity by mutated SEC2 correlate with its improved antitumour potency? Context: To investigate the improved antitumour activity of SAM-3 compared with recombinant staphylococcal enterotoxins C2 (rSEC2). Methylthiazol tetrazolium and flow cytometry assays showed that the antitumour activity of SAM-3 in vivo was improved because of enhanced T-cell stimulating potency, resulting in massive activation of T cells, particularly CD4(+) and CD8(+) T cells, and subsequent cytokine release. Quantitative real-time PCR assay showed that despite similar Vβ specificities induced by rSEC2 and SAM-3, the quantities of activated T cells bearing specific Vβin vitro were different.
assistant
The results strongly suggested that the increased SAM-3-T-cell receptor (TCR) binding affinity contributed to massive T-cell activation and cytokine release, substantially amplifying antitumour immune response in vivo. Final Decision: yes