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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Antigens, CD34, Carcinoma, Cells, Cultured, Female, Humans, Immunohistochemistry, Leukocytes, Mononuclear, Mice, Mice, Nude, Neovascularization, Pathologic, Ovarian Neoplasms.
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Do [ Peripheral blood CD34+-positive mononuclear cells participate in neovasculogenesis of human ovarian epithelial carcinoma ]? Context: To observe whether endothelial cell (EC) progenitors (CD(34)(+)-positive mononuclear cells) participated in neovasculogenesis of ovarian epithelial carcinoma through in vitro and in vivo experiments, and to explore the mechanism of tumor neovasculogenesis. CD(34)(+)-positive mononuclear cells were isolated from peripheral blood of ovarian epithelial carcinoma patients by means of magnetic beads coated with antibody to CD(34)(+), plated on culture dishes coated with human fibronectin in endothelium medium, and examined by using RT-PCR, fluorescence-activated cell sorting (FACS) and nitric oxide (NO) assay kit for the expression of EC lineage-markers. EC-like cells were labeled with DiI ex vivo, and injected into immunodeficiency mice model with transplanted hypodermic SKOV3 by caudal vein. After 4 - 6 weeks, the tumor was resected and examined by confocal microscopy, and immunohistochemistry. In vitro, CD(34)(+)-positive mononuclear cells differentiated into ECs. In animal models of SKOV3, EC progenitors (CD(34)(+)-positive mononuclear cells) incorporated into sites of neovasculogenesis in tumor, 4 - 6 weeks later DiI-labeled cells incorporated into capillaries and small arteries.
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The neovasculogenesis in human ovarian epithelial carcinoma involves angiogenesis and vasculogenesis. Final Decision: yes