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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Chemokine CXCL2, Cyclooxygenase 2, Ethanol, Female, Gastric Mucosa, Macrophages, Mice, Mice, Inbred C3H, Mice, Inbred C57BL, Signal Transduction, Stomach Diseases, Toll-Like Receptor 4.
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Does toll-like receptor 4 participate in gastric mucosal protection through Cox-2 and PGE2? Context: To elucidate the role of Toll-like receptors (TLRs) in gastric cytoprotection after ethanol injury. C57BL/6J, C3H/HeOuJ and C3H/HeJ mice were used. All mice were killed 4h after ethanol administration. TLR4, cyclooxygenase-2 (Cox-2) and prostaglandin E(2) (PGE(2)) expression were measured by immunohistochemistry, western blotting and enzyme-linked immunosorbent assay (ELISA) separately. The expression of TLR4 increased in C57BL/6J mice stomach 4h after ethanol injury. The cells expressing TLR4 included Cox-2 expressing cells and macrophages. The injury in C3H/HeJ mice was more severe than in C3H/HeOuJ mice 4h after ethanol injury. The expression of Cox-2 and PGE(2) only increased in C3H/HeOuJ mice. The number of macrophages and the expression of macrophage-inflammatory protein-2 (MIP-2) also increased only in C3H/HeOuJ mice.
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TLR4 signal is activated in mice stomach 4h after ethanol injury. The protective effects of TLR4 signalling are mediated through the induction of Cox-2 expression and the production of PGE(2). Final Decision: yes