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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, RESULTS. Meshes: Adolescent, Antibodies, Neutralizing, Antibodies, Viral, Child, Cohort Studies, Dengue, Dengue Virus, Female, Humans, Male, Neutralization Tests, Thailand, Viral Plaque Assay.
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Does plaque reduction neutralization antibody test accurately predict protection against dengue infection in Ratchaburi cohort , Thailand? Context: The plaque reduction neutralization test (PRNT) is currently the best and most widely accepted approach to measuring virus-neutralizing and protective antibodies to dengue virus, and in assessing the immunogenicity of a dengue vaccine. However, the correlation between presence of dengue-neutralizing antibody and protection from infection is not absolute. In a cohort study in Ratchaburi Province, Thailand, 48 subjects with serologically confirmed symptomatic dengue infection were tested for pre-existing dengue neutralizing antibody using PRNT. Nine subjects had quite high pre-existing PRNT50 titers (titer >90) to subsequent infecting dengue serotypes, but still had symptomatic infections.
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This report provides evidence that PRNT may not be a good test for predicting protection against subsequent dengue infection. Final Decision: no