system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: AC133 Antigen, Animals, Antigens, CD, Cell Line, Glycoproteins, Green Fluorescent Proteins, Luminescent Proteins, Membrane Glycoproteins, Peptides, Plasmids, Recombinant Fusion Proteins, Transfection.
user
Is [ Fudenine , relative to blood glucose-control , a novel membrane protein ]? Context: To determine whether Fudenine is a novel membrane protein. Green fluorescence protein(GFP) was used to localize Fudenine in vivo. GFP, as a control, was targeted to cytoplasm. Epithelial cell, CBRH7919, and non-epithelial cell, L-6TG, were cultured and transiently transfected by using the lipofectamine reagent. After 48 h, intact cells were examined with fluorescence microscope for Fudenine. Reporter plasmid pEGFP-N1, as a control, was expressed and localized to cytoplasm. But Fudenine, driven by the cytomegalovirus promoterenhancer contained in the pEGFP-N1 vector, was overexpressed and targeted to cellular membrane.
assistant
Fudenine is a novel membrane protein. It may play the similar role with its homologues AC133 antigen and prominin in human and mouse, respectively. It might be involved in signaling transduction and regulate blood glucose metabolism in vivo. Final Decision: yes