system
Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Cell Adhesion, Cells, Cultured, Chickens, Culture Media, Conditioned, Dose-Response Relationship, Drug, Edetic Acid, Enzyme Activation, Epithelial Cells, Extracellular Matrix, Gelatin, Lens, Crystalline, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Matrix Metalloproteinase Inhibitors, Matrix Metalloproteinases, Phenylmercuric Acetate, Plasminogen, Sensitivity and Specificity, Time Factors, Transforming Growth Factor beta.
user
Is matrix metalloproteinase secretion stimulated by TGF-beta in cultured lens epithelial cells? Context: To determine if TGF-beta regulates the expression of metalloproteinases in chick lens annular pad cells. The activity of secreted matrix metalloproteinases was examined with gelatin zymography in primary cultures exposed to TGF-beta. Metalloproteinases with electrophoretic mobilities corresponding to MMP2 and MMP9 were tentatively identified. Activated, processed forms of the two metalloproteinases were also observed. Plasminogen activators potentially capable of initiating metalloproteinase cascades were concomitantly elicited. Metalloproteinase secretion was shown to be specific for TGF-beta stimulation and independent of substrate composition.
assistant
These results indicate that TGF-beta-mediated processes could be responsible for localized lens capsular heterogeneity, establishing a substrate suitable for cell migration or the release of matrix-bound factors which influence the terminal differentiation of lens cells. Final Decision: yes