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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Aniline Compounds, Animals, Antineoplastic Agents, Apoptosis, Biphenyl Compounds, Blotting, Western, Bone Neoplasms, Cell Proliferation, Chondrosarcoma, Disease Models, Animal, Humans, Male, Mice, Mice, Inbred NOD, Mice, SCID, Middle Aged, Molecular Mimicry, Molecular Targeted Therapy, Nitrophenols, Peptide Fragments, Piperazines, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Sulfonamides, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, bcl-2 Homologous Antagonist-Killer Protein, bcl-2-Associated X Protein.
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Do bH3 mimetics inhibit growth of chondrosarcoma -- a novel targeted-therapy for candidate models? Context: Chondrosarcoma is refractory to conventional chemotherapy. BH-3 mimetics ABT-737 and ABT-263 are synthetic small-molecule inhibitors of anti-apoptotic proteins B-cell lymphoma-2 (Bcl2) and Bcl-xL, which play a critical role in survival of chondrosarcoma cells. Chondrosarcoma cell lines SW-1353 and CS-1 were used as the disease model. We used immunoblotting to assess the expression of target molecules Bcl2 and Bcl-xL, and the apoptotic inducers Bcl2-associated X (Bax) and Bcl2-antagonist/killer (Bak). In vitro growth inhibition by BH-3 mimetics was confirmed by photomicroscopic cell counting and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay. Apoptotic induction was confirmed by Enzyme-Linked ImmunoSorbent Assay (ELISA). In vivo growth inhibition was assessed in a non-obese diabetic/severe combined immunodeficient (NOD/SCID) mouse model. Expression of the target and effector molecules was confirmed in chondrosarcoma cell lines. BH3 mimetics significantly inhibited cell growth and induced apoptosis in vitro. Administration of ABT-263 inhibited chondrosarcoma growth and improved survival in a mouse model.
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BH3 mimetics represent a novel treatment modality for chondrosarcoma. Final Decision: yes