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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Animals, Base Sequence, Breast Neoplasms, Cell Line, Tumor, Cell Proliferation, Chromatin Immunoprecipitation, DNA-Binding Proteins, Disease Models, Animal, Electrophoretic Mobility Shift Assay, Female, Gene Expression Regulation, Neoplastic, Genes, Neoplasm, HeLa Cells, Hep G2 Cells, Humans, Liver Neoplasms, Lung Neoplasms, Mice, Mice, Nude, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, RNA Interference, RNA, Long Noncoding, RNA, Small Interfering, Sequence Deletion, Sp1 Transcription Factor, Transcription, Genetic, Transcriptional Activation.
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Does sp1-mediated transcriptional regulation of MALAT1 play a critical role in tumor? Context: MALAT1 was discovered as a prognostic marker for lung cancer metastasis and has been found upregulated in many types of tumor, but its transcriptional regulation mechanism in tumors remains unclear. A deletion analysis of MALAT1 promoter region was performed to find the cis elements that were critical for the transcriptional activation of MALAT1 gene. Reporter gene assays were employed to analyze the effect of Sp1 on the promoter activity of MALAT1 gene. The binding activity of Sp1 with the promoter of MALAT1 gene was examined by EMSA and ChIP assay. Effects of Sp1 on regulation of MALAT1 were analyzed by RNA interference in vitro and in vivo mouse model. By means of luciferase assay, Sp1 was found to activate the promoter of the human MALAT1 gene. The binding of Sp1 to this region was also detected by electrophoretic mobility shift and chromatin immunoprecipitation assays. Sp1 knockdown also decreased the MALAT1 and inhibited A549 lung cancer cells' growth and invasion in vitro. Furthermore, knockdown of Sp1 also mimicked the inhibition of MALAT1 in A549 lung cancer cells' growth and metastasis in vivo.
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Taken together, our data suggest that upregulation of MALAT1 was mediated by the transcription factor Sp1 in A549 lung cancer cells, and Sp1 could be therapeutic target for cancer. Final Decision: yes