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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: 3T3 Cells, Alkaline Phosphatase, Animals, Cell Differentiation, Cell Proliferation, Core Binding Factor Alpha 1 Subunit, Gene Expression Regulation, Developmental, Mesenchymal Stem Cells, Mice, Osteoblasts, Osteoprotegerin, Proto-Oncogene Proteins c-akt, Recombinant Proteins, Smad1 Protein, Tooth Movement Techniques, Transfection, Up-Regulation.
user
Does overexpression of osteoprotegerin promote preosteoblast differentiation to mature osteoblasts? Context: The hypothesis of the present study is that overexpression of osteoprotegerin (OPG) promotes preosteoblast maturation. The preosteoblast cell line MC3T3-E1 was transfected with OPG overexpression. OPG expression was confirmed by enzyme-linked immunosorbent assay (ELISA) and Western blot. Changes in the transcription factors in OPG-expressing cells were assessed by real-time polymerase quantitative polymerase chain reaction (RT-qPCR). Alkaline phosphate (ALP) expression was measured by ELISA. The success of stable transfection of MC3T3-E1 cells with OPG overexpression was confirmed by MoFlow sorting followed by G418 selection. RT-qPCR showed that expression of RunX2, the most important osteoblast differentiation controlling factor, was suppressed. Smad1 and Akt1, as well as ALP, were upregulated in the OPG overexpressing cells.
assistant
Results from the present study provide evidence that overexpression of OPG in preosteoblasts promotes its differentiation into mature osteoblasts. Final Decision: yes