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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Apoptosis, Cell Line, Tumor, Cell Proliferation, Cell Survival, Gene Expression Regulation, Humans, Interleukin-1beta, MicroRNAs, NF-kappa B, PTEN Phosphohydrolase, Stomach Neoplasms, Up-Regulation.
user
Does [ MiR-425 up-regulation induced by interleukin-1β promote the proliferation of gastric cancer cell AGS ]? Context: To evaluate the mechanism of interleukin-1 (IL-1) in tumorigenesis. The miRNA expression was profiled with the Exiqon miRCURY(TM) LNA Array System. And the miRecords algorithm and luciferase reporter assays were employed for identifying the targets of miR-425. Cell proliferation assay was performed with CCK8. Cell apoptosis assay was performed with flow cytometry. IL-1β induced the up-regulation of miR-425, a negative expression regulator of phosphatase and tensin homolog (PTEN). An increase in miR-425 depended upon IL-1β-induced NF-kappaB activation.Repression of PTEN by miR-425 promoted gastric cancer cell proliferation.
assistant
There is a critical role for NF-kappa B-dependent up-regulation of miR-425. And it represents a new pathway for the repression of phosphatase and tensin homolog activation and the promotion of cell survival upon IL-1β induction. Final Decision: yes