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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Aged, Aged, 80 and over, Animals, Antineoplastic Agents, Apoptosis, Cell Differentiation, Cell Proliferation, Cell Separation, Chemotaxis, Leukocyte, Enzyme-Linked Immunosorbent Assay, Female, Flow Cytometry, Humans, Immunohistochemistry, Interleukins, Male, Mice, Mice, Inbred NOD, Mice, SCID, Middle Aged, Multiple Myeloma, Neoplasm Staging, Neovascularization, Pathologic, Osteoblasts, Osteoclasts, Polymerase Chain Reaction, Xenograft Model Antitumor Assays.
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Does interleukin-27 act as multifunctional antitumor agent in multiple myeloma? Context: Multiple myeloma (MM) derives from plasmablast/plasma cells that accumulate in the bone marrow. Different microenvironmental factors may promote metastatic dissemination especially to the skeleton, causing bone destruction. The balance between osteoclast and osteoblast activity represents a critical issue in bone remodeling. Thus, we investigated whether interluekin-27 (IL-27) may function as an antitumor agent by acting directly on MM cells and/or on osteoclasts/osteoblasts. The IL-27 direct antitumor activity on MM cells was investigated in terms of angiogenesis, proliferation, apoptosis, and chemotaxis. The IL-27 activity on osteoclast/osteoblast differentiation and function was also tested. In vivo studies were done using severe combined immunodeficient/nonobese diabetic mice injected with MM cell lines. Tumors from IL-27- and PBS-treated mice were analyzed by immunohistochemistry and PCR array. We showed that IL-27 (a) strongly inhibited tumor growth of primary MM cells and MM cell lines through inhibition of angiogenesis, (b) inhibited osteoclast differentiation and activity and induced osteoblast proliferation, and (c) damped in vivo tumorigenicity of human MM cell lines through inhibition of angiogenesis.
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These findings show that IL-27 may represent a novel therapeutic agent capable of inhibiting directly MM cell growth as well as osteoclast differentiation and activity. Final Decision: yes