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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Antibodies, Monoclonal, Antimetabolites, Antineoplastic, Apoptosis, Cytarabine, DNA Fragmentation, Flow Cytometry, HL-60 Cells, Humans, Immunoglobulin M, fas Receptor.
user
Does anti-Fas IgM monoclonal antibody enhance apoptosis induced by low-dose cytosine arabinoside? Context: Although low-dose cytosine arabinoside (LD-Ara-C) therapy has been accepted as an effective treatment for patients with acute non-lymphocytic leukemia (ANLL) transformed from myelodysplastic syndromes or elderly patients with ANLL, the anti-leukemic mechanism remains to be resolved. Recently, the potential role of the Fas/Fas ligand system in chemotherapeutic drug-induced apoptosis has been studied. In the present study the relationship between the anti-leukemic effect of LD-Ara-C and the Fas/Fas ligand system was examined. The human myeloid leukemia cell line HL60 was treated with LD-Ara-C in combination with anti-Fas IgM MoAb, and apoptosis in the treated cells was estimated by morphological observation, DNA electrophoresis and flow cytometry. Simultaneously, changes in Fas antigen expression on cells treated with LD-Ara-C were investigated. Only limited apoptosis was observed following treatment with LD-Ara-C alone or anti-Fas MoAb alone; however, a synergistic increase in apoptosis was observed by treatment with the MoAb in combination with pretreatment with LD-Ara-C. LD-Ara-C induced a slight but consistent increase in the expression of Fas antigen on the treated cells. Moreover, the expression of Fas antigen was enhanced by repeated administration of LD-Ara-C.
assistant
These findings suggest the possible involvement of the Fas/Fas ligand system in the anti-leukemic effect of LD-Ara-C therapy. Final Decision: yes