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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Carcinoma, Non-Small-Cell Lung, Cell Cycle, Cell Death, Cell Line, Tumor, Cisplatin, Deoxyglucose, Drug Hypersensitivity, Flow Cytometry, Heat-Shock Proteins, Humans, JNK Mitogen-Activated Protein Kinases, Lung Neoplasms, NF-kappa B, Tunicamycin, Up-Regulation.
user
Does gRP78 up-regulation lead to hypersensitization to cisplatin in A549 lung cancer cells? Context: GRP78 is one of the stress proteins linked to different functions in the cell. Previous reports have shown opposing functions of GRP78 in relation to drug resistance/sensitivity. In the current study, we examined the role of GRP78 in cisplatin-treated A549 cells. GRP78 was over-expressed in A549 cells with 2-deoxyglucose (2-dG) or tunicamycin (TM) treatments for 48 h and subsequently exposed to cisplatin for 2 h. Viability of these cells was determined at 0, 12, 24, 36 and 48 h afterwards. We showed that A549 cells are hypersensitized to cisplatin following a transient GRP78 up-regulation. This hypersensitization is caused by the activation of JNK pathway and NF-κB, leading to early onset of apoptosis.
assistant
Induction of GRP78 can be used as a potential tool to overcome drug resistance in lung cancer cells. Final Decision: yes