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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Animals, Body Weight, Candida albicans, Candidiasis, Cells, Cultured, Central Nervous System, Cholecalciferol, Cytokines, Dendritic Cells, Disease Models, Animal, Down-Regulation, Encephalomyelitis, Autoimmune, Experimental, Female, Forkhead Transcription Factors, Indoleamine-Pyrrole 2,3,-Dioxygenase, Lymph Nodes, Mice, Mice, Inbred C57BL, Myelin-Oligodendrocyte Glycoprotein, Vitamins.
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Does tolerogenic Vaccination with MOG/VitD overcome Aggravating Effect of C. albicans in Experimental Encephalomyelitis? Context: Multiple sclerosis (MS) is an immune-mediated demyelinating disorder of the central nervous system (CNS). We described that Candida albicans (Ca) aggravates experimental autoimmune encephalomyelitis (EAE) that is a model to study MS. We also observed that vaccination with a myelin peptide (MOG) in the presence of vitamin D (VitD) protected mice against EAE. In this work, we investigated whether Ca infection interferes with the efficacy of this vaccine. EAE was induced in C57BL/6 female mice previously vaccinated with MOG+VitD and then infected 3 days before encephalomyelitis induction. Vaccination was able to control EAE development in infected mice. These animals gained weight, and only a few progressed to very low clinical scores. Protection was confirmed by a lower inflammatory infiltration in the CNS and was also associated with a reduced production of encephalitogenic cytokines by spleen and CNS cell cultures. The elevated percentage of CD25(+) FoxP3(+) cells suggests that regulatory T cells are involved in the protection. Adoptive transfer of splenocytes from mice vaccinated with MOG+VitD supports the view that protection is mediated by immunoregulatory cells.
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Together, these experiments provide evidence demonstrating that EAE can be prevented by the inverse vaccination with MOG+VitD even in the presence of a disease-aggravating infectious agent. Final Decision: yes