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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, METHODS, RESULTS. Meshes: Bacterial Outer Membrane Proteins, Drug Resistance, Multiple, Bacterial, Escherichia coli, Escherichia coli Proteins, Fluorometry, Gene Deletion, Gene Expression Regulation, Bacterial, Hydrocarbons, Fluorinated, Lipoproteins, Membrane Transport Proteins, Multidrug Resistance-Associated Proteins, Quorum Sensing.
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Do fluorinated Beta-diketo Phosphorus Ylides Are Novel Efflux Pump Inhibitors in Bacteria? Context: One of the most important resistance mechanisms in bacteria is the increased expression of multidrug efflux pumps. To combat efflux-related resistance, the development of new efflux pump inhibitors is essential. Ten phosphorus ylides were compared based on their MDR-reverting activity in multidrug efflux pump system consisting of the subunits acridine-resistance proteins A and B (AcrA and AcrB) and the multidrug efflux pump outer membrane factor TolC (TolC) of Escherichia coli K-12 AG100 strain and its AcrAB-TolC-deleted strain. Efflux inhibition was assessed by real-time fluorimetry and the inhibition of quorum sensing (QS) was also investigated. The relative gene expression of efflux QS genes was determined by real-time reverse transcriptase quantitative polymerase chain reaction. The most potent derivative was Ph
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Phosphorus ylides might be valuable EPI compounds to reverse efflux related MDR in bacteria. Final Decision: yes