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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, METHODS, METHODS, METHODS, METHODS, RESULTS. Meshes: Adult, Cells, Cultured, Chorionic Gonadotropin, Coculture Techniques, Corpus Luteum, Estradiol, Female, Follicular Atresia, Follicular Fluid, Granulosa Cells, Humans, Membrane Glycoproteins, Osmolar Concentration, Progesterone, Proteoglycans, Syndecan-4.
user
Is ryudocan expression by luteinized granulosa cells associated with the process of follicle atresia? Context: To evaluate the presence of ryudocan in follicular fluid (FF) and its possible correlation with FF E(2) and P, and to study the levels of ryudocan in granulosa-lutein cells stimulated with hCG. Controlled clinical study and in vitro experiment. University teaching hospital. One hundred seven patients undergoing IVF. The FF and granulosa-lutein cells were aspirated from follicles 34 hours after an ovulatory gonadotropin bolus. FF ryudocan, E(2), and P levels as well as hCG-mediated induction of ryudocan. Ryudocan was abundant in the FF; the concentration of ryudocan in human FF was estimated to be 305.5 +/- 200.8 ng/mL (mean +/- SD). Atretic follicles had higher concentrations of ryudocan (559.1 +/- 156.5 ng/mL). FF ryudocan levels were inversely correlated with FF E(2) (r = -0.5023) and P concentrations (r = -0.4459). A detectable amount of ryudocan was found in pooled granulosa-lutein cells. Ryudocan production was augmented by surge levels of hCG.
assistant
Ryudocan is expressed in luteinized granulosa cells in vitro. The higher concentrations of ryudocan in FF of atretic follicles suggest an involvement of ryudocan in the process of atresia. Final Decision: yes