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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: OBJECTIVE, METHODS, RESULTS. Meshes: Androstadienes, Animals, Anoikis, Antibodies, Blotting, Western, Cadherins, Cell Adhesion, Cell Communication, Cell Line, Tumor, Cell Proliferation, Cell Survival, Enzyme Inhibitors, Hepatocyte Growth Factor, Humans, Imidazoles, Mice, Mice, Inbred BALB C, Mice, Nude, Pancreatic Neoplasms, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-akt, Pyridines, Signal Transduction, Wortmannin, Xenograft Model Antitumor Assays, p38 Mitogen-Activated Protein Kinases.
user
Does hepatocyte growth factor inhibit anoikis of pancreatic carcinoma cells through phosphatidylinositol 3-kinase pathway? Context: Escape from anoikis, apoptosis induced by loss of cell-cell or cell-extracellular matrix interactions, is important in tumor invasion and metastasis. Hepatocyte growth factor (HGF) is known to play a pivotal role in pancreatic carcinomas. This study aimed to determine the antianoikis effect of HGF in pancreatic carcinoma cells. Antianoikis effect of HGF was evaluated in human pancreatic carcinoma cells in nonadherent culture with or without anti-E-cadherin antibody. Signal pathways were investigated by Western blot analysis and inhibition assay using inhibitors for phosphatidylinositol 3-kinase and p38. Pancreatic carcinoma cells underwent anoikis in nonadherent culture. However, some of the carcinoma cells survived by forming aggregations in suspension. Anti-E-cadherin antibody dissociated the aggregations, and the separated cells underwent additional anoikis. Hepatocyte growth factor inhibited anoikis irrespective of E-cadherin-mediated cell-cell contact. Inhibition of the phosphatidylinositol 3-kinase/Akt pathway abolished the antianoikis effect of HGF. Phosphorylation of Akt was induced by HGF, and the phosphorylated Akt persisted even when E-cadherin was inhibited.
assistant
Hepatocyte growth factor inhibits anoikis of pancreatic carcinoma cells through phosphatidylinositol 3-kinase pathway in which activation of Akt may be involved. It is thus supposed that HGF may have a potent role in invasion and metastasis of pancreatic carcinoma cells by exerting its antianoikis effect. Final Decision: yes