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Medical AI Inquiry: You was designed to assist in medical decision-making based on the provided user questions. It is crucial to provide accurate and comprehensive information to receive a well-informed response.  Labels: BACKGROUND, OBJECTIVE, METHODS, RESULTS. Meshes: Apoptosis, Cell Survival, Collagen Type I, Extracellular Matrix, Fibroblasts, Humans, Intercellular Signaling Peptides and Proteins, Skin, Skin Aging, Tissue Inhibitor of Metalloproteinase-1, Ultraviolet Rays.
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Does plasma Rich in Growth Factors inhibit Ultraviolet B Induced Photoageing of the Skin in Human Dermal Fibroblast Culture? Context: Ultraviolet irradiation is able to deeply penetrate into the dermis and alter fibroblast structure and function, leading to a degradation of the dermal extracellular matrix. The regenerative effect of plasma rich in growth factors (PRGF) on skin ageing was investigated using UVB photo-stressed human dermal fibroblasts as an in vitro culture model. PRGF was assessed over the main indicative features of ultraviolet B irradiation, including ROS formation, cell viability and death detection, apoptosis/necrosis analysis and biosynthetic activity measurement. Four different UV irradiation protocols were tested in order to analyze the beneficial effects of PRGF. Ultraviolet irradiation exhibited a dose dependent cytotoxicity and dose of 400mJ/cm2 was selected for subsequent experiments. PRGF increased the cell viability and decreased the cell death comparing to the non-treated group. The apoptosis and necrosis were significantly lower in PRGF treated fibroblasts. ROS production after UV irradiation was significantly reduced in the presence of PRGF. Procollagen type I, hyaluronic acid and TIMP-1 levels were higher in the when treated with PRGF.
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This preliminary in vitro study suggests that PRGF is able to prevent UVB derived photo-oxidative stress and to diminish the cell damage caused by ultraviolet irradiation. Final Decision: yes