exonuclease I
First, 5 uL PCR products containing the mutation were incubated with 1.5 µL enzyme mixtures, containing 5 units exonuclease I (ExoI) (USB Corporation) and 1 unit shrimp alkaline phosphatase (SAP) (USB Corporation), incubated 37° C for 15 minutes, followed by 80o C for 15 minutes.
First, 5 uL PCR products containing the mutation were incubated with 1 .5 µL enzyme mixtures, containing 5 units exonuclease I ( ExoI ) ( USB Corporation ) and 1 unit shrimp alkaline phosphatase ( SAP ) ( USB Corporation ), incubated 37° C for 15 minutes, followed by 80o C for 15 minutes .