Prior to his appointment in the Parkos lab at the University of Michigan, he worked as a physician scientist in the Department of General Surgery at the University Hospital of Wuerzburg, Germany, and was affiliated with the group of Dr. Nicolas Schlegel. In his previous studies he focused on endothelial barrier disruption caused by pro-inflammatory and/or septic stimuli in-vitro and in-vivo and, secondly, on regulation of epithelial barrier homeostasis in inflammatory bowel diseases and septic conditions. Dr. Flemming currently studies the multistep process of transepithelial migration of leukocytes under inflammatory conditions using intravital microscopy.