Elizabeth I of England was childless.
Elizabeth I of England . Anne 's marriage to Henry VIII was annulled , and Elizabeth was declared illegitimate . It was expected that Elizabeth would marry and produce an heir to continue the Tudor line . She never did , despite numerous courtships . Union of the Crowns . The Union of Crowns followed the death of Elizabeth I of England -- the last monarch of the Tudor dynasty , who was James 's unmarried and childless first cousin twice removed .