Macbeth was first published in the Folio of 1623, by Shakespeare.
First Folio . Printed in folio format and containing 36 plays ( see list of Shakespeare 's plays ) , it was prepared by Shakespeare 's colleagues John Heminges and Henry Condell . Macbeth . It was first published in the Folio of 1623 , possibly from a prompt book , and is Shakespeare 's shortest tragedy .