Harley Quinn is exclusively a film character.
Harley Quinn . She later appeared in DC Comics ' Batman comic books , with the character 's first comic book appearance in The Batman Adventures # 12 ( September 1993 ) . The character made her live-action cinematic debut in the 2016 film Suicide Squad , portrayed by Margot Robbie . Harley Quinn Smith ( born June 26 , 1999 ) is an American actress .