Evaluation of valosin containing protein (P97) as a cancer biomaker in canine lymphomas
Lymphoma is one of the common malignancies in both dogs and humans. Annually, an important number of canine patients develop this aggressive cancer and a majority succumbs to the disease within one year. In recent years, canine lymphoma has been increasingly recognized as an excellent model for the disease in humans, especially with regards to the molecular biology of the disease. Consequently, research targeted at canine lymphoma benefits not only dogs but the field of human oncology as well. Among the most desirable diagnostic and screening tests for lymphoma is the measurement of cancer biomarkers. They have the advantage of being minimally invasive, simple, and inexpensive. Thus, with the aim of evaluating valosin containing protein (VCP) as a cancer biomarker in canine B and T-cell lymphomas, we first performed western blots on sera and tumor tissue of dogs with lymphoma and then immunohistochemical analysis on low, intermediate and high-grade tumors. To further determine VCP expression in cancer cells, we also examined VCP levels by immunoblotting in 3 tumor cell lines: CLBL-1, CL-1, and 17-71. High-grade B-cell lymphomas had significantly increased levels of VCP compared to low-grade tumors (P < 0.05). Additionally, we detected a corresponding accumulation of VCP in tumor cells lines compared to peripheral blood mononuclear cells (PBMCs) (P < 0.05). In contrast, VCP levels were not elevated in sera of dogs with lymphoma compared to healthy controls. These results suggest that VCP positively correlates with malignancy in canine B-cell lymphomas. We conclude that VCP merits further investigation to determine its potential as a clinically useful prognosis biomarker for canine B-cell lymphoma.
La proteine VCP Lymphome canine Lymphome à cellules B Valosin containing protein (p97) Cancer biomarker Canine Lymphoma B-cell lymphoma Veterinary oncology Dog Malignancy