Une nouvelle stratégie de vaccination contre Salmonella Enteritidis, chez le poulet de chair : «les vésicules externes de membrane bactérienne»
Chicken meat consumption is considered as an important risk factor in Salmonella Enteritidis foodborne outbreaks. To better control SE in poultry, we are suggesting outer membrane vesicles (OMVs) as a potential vaccine. Previous studies have indeed shown that OMVs can be immunogenic and they have been used as vaccines. The objective of this project was to produce, isolate and purify such vesicles from a SE field strain, isolated from the province of Québec, then to do a first characterization. OMVs production was performed in normal growth conditions and under stress conditions. Stress consisted of antibiotic addition to the growth medium as well as growth in an acid minimal medium. Examination of the resulting supernatants by electron microscopy confirmed the presence of OMVs in all growth conditions. The production protocol in normal growth conditions was chosen for isolation, and purification of the vesicles was then performed by density gradient. Initial characterization of OMVs was made by migration on an electrophoresis gel (SDS-PAGE) and their protein profile was compared with the SE whole-cell lysate and SE outer membrane proteins (OMPs). Finally, quantification in lipopolysaccharides content (LPS) was done. We have produced, isolated and purified OMVs ready to be tested in vivo to verify for their immunogenic and protective effects in chickens.
Salmonella Enteritidis (SE) Vaccin Outer Membrane Vesicles (OMVs) Vaccination Poultry