Analyse de la localisation génomique et identification de nouvelles fonctions des sous-unités Rpb4/Rpb7 de l’ARN polymérase II et des facteurs TFIIF, TFIIS et UBR5
Biochemical, genetic and structural studies made over the last years bring a new view on the RNA polymerase II (Pol II) machinery and the process by which it decodes the genetic information. They provided new insights into the diversity of the transcriptional regulation mechanisms, and on the role played by the general transcription factors (GTFs).  The studies presented in this thesis provide new evidence on the role of human GTFs in the regulation of different stages of transcription.  In the first part of the thesis, we investigated the function of the human Pol II and GTFs in living cells, by systematically analyzing their genomic location. The location profiles obtained by chromatin immunoprecipitation (ChIP) of TAP (tandem-affinity purification) tagged versions of these factors indicate new in vivo functions for several components of this machinery, and for structural elements of the Pol II. These results suggest that TFIIF and the heterodimer Rpb4–Rpb7 have a specific function during the elongation stage in vivo. Additionally, our study offers for the first time a general picture of GTFs function during the Pol II transcription reaction in live mammalian cells, and provides a framework to uncover new regulatory hubs.   Secondly, we report on the identification of a new function of the factor TFIIS in the regulation of CDK9, the kinase subunit of the Positive Transcription Elongation Factor b (P-TEFb). We identify two interaction partners for TFIIS, namely CDK9 and the E3 ubiquitin ligase UBR5. We show that UBR5 catalyzes the ubiquitination of CDK9 in vitro. Moreover, the polyubiquitination of CDK9 in human cells is dependent upon both UBR5 and TFIIS, and does not signal its degradation. We also show that UBR5, CDK9 and TFIIS co-localize along specific regions of the  fibrinogen (FBG) gene, and that the overexpression of TFIIS increases the occupancy of CDK9 along this gene in a UBR5 dependant manner. We propose a new function of TFIIS in the transition between initiation and elongation stages, by regulating the stability of the early CDK9-Pol II transcribing complexes.    Key words: chromatin immunoprecipitation, general transcription factors, tandem-affinity purification, RNA polymerase II, Rpb4–Rpb7 heterodimer, transcription factor IIF (TFIIF), transcription factor IIS (TFIIS), UBR5 ubiquitin ligase, Positive Transcription Elongation Factor b (P-TEFb), CDK9 ubiquitination.
Tandem-affinity purification Positive-Transcription Elongation Factor b (P-TEFb)