Caractérisation de la réponse adaptative humorale contre le streptocoque du groupe B
Group B Streptococcus (GBS) is an agent of septicemia and meningitis in newborns but also in adults. A serological reaction directed against the polysaccharide capsule (CPS) allows to differentiate 10 GBS serotypes, including serotype III which is the most frequently isolated in cases of meningitis. Currently the effectiveness of the only available treatment, intrapartum antibiotic prophylaxis, is controversial. To improve prevention strategies, this study aims to better understand the interactions between GBS and the development of the adaptive response, a subject that is poorly documented. This study evaluated, by flow cytometry (FACS), the splenic subpopulations of B lymphocytes (LB) involved following systemic GBS infection in an in vivo model. This study also evaluated the serum anti-GBS antibody response and against its purified capsule as well as the formation of splenic germinal centers (GCs) in the context of multiple GBS infections. Results suggest that the first infection stimulates the production of antibodies against GBS III but little against its capsule. Furthermore, results suggest that GBS activates B cell differentiation by inducing the production of GCs, which are linked to triggering a memory response allowing better control in subsequent infections. Despite its low immunogenicity, the CPS does not appear to interfere with the development of adaptive humoral immunity against the bacteria. Therefore, the production of antibodies against GBS III, involving class switching, would recognize different epitopes from those found on its capsule.
infections multiples Group B Streptococcus capsular polysaccharide multiple infections humoral response germinal centers class switching in vivo