Instrumentation et méthodes d’analyse appliquées à l’évaluation du développement rétinien chez le souriceau par tomographie par cohérence optique
This master thesis is including a manuscript submitted for publication and is divided into two sections. In particular, the document describes the development of optical instrumentation and methodological tools to study the retinal development in an animal model through optical coherence tomography (OCT). The first section is divided into two chapters. Chapter 1 describes the theoretical basis and the problem to solve. Chapter 2 describes the tools developed or adapted to enable the performance of the experiments. In particular, this chapter details the optical system designed to allow the optimal focalization of the light beam onto the retina, which maximizes the signal and minimizes the impact of spherical aberrations and light dispersion on image quality. Also, the processing algorithm used to segment retinal tissues from OCT tomography is discussed. The second section is divided into two chapters. Chapter 3 is a manuscript that was submitted for publication to Investigative Ophthalmology & Visual Science. This work describes the growth and maturation of specific retinal layers in the wild type mouse (129S1/SvImJ) between 7 and 21 days of life. The manuscript also describes the image processing and segmentation algorithm that allows the quantification of the thickness of the layers over time. Then, Chapter 4 describes specific research projects where these OCT imaging tools can be applied. Specifically, this chapter shows the potential to study retinal developmental diseases as well as perfusion methods.
Optical coherence tomography Retinal development Mouse pup Image processing Angiography Physiology