L’influence de mitochondries exogènes : changements phénotypiques chez Chrosomus eos
The mito-nuclear interactions are at the center of the mitochondrial functions, such as energy production. Despite their importance, little is known about how the influence of the mitochondrial genome on the nuclear genes expressions. The cybrids (cytoplasmic hybrids) can present phenotype modification (adaptive or not) caused by the mito-nuclear interactions. In this study, different mitotype of Cybrids of the Chrosomus eos species were used as model to further study the interaction between a cell’s genomes. This complex of species has two types of cybrids, the mitochondria of which originates from two different glacial refugia : Atlantic and Mississippian. This set up of this study was a sympatric lake, to minimise the environmental factor. Allopatric population were also separately analysed to supplement past studies. To accomplish these goals, multiple methods were used, assessing also multiple levels of gene expression: Methylation, transcription, protein and enzymatic activity. Differences between mitotypes were observed, but of lesser magnitude then the environmental factor. Thus, this result suggest that the differences associated to the sympatric mitotypes drive a deference in environment colonization, which would create the inter-lake intra-mitotype similarity observed in past studies. To assess the transcription of mitotype, a transcriptomic reference was produced. This reference offers a lot of information and future applications.
Chrosomus eos RNA-seq Transcriptomic Cybrid methylome enzymatic activity