CROSS REFERENCE TO RELATED APPLICATIONS

The present application is a U.S. national phase application of PCT/KR2019/009295, filed Jul. 25, 2019, which claims priority to Korean Application No. 10-2018-0151042, filed Nov. 29, 2018.

STATEMENT REGARDING SEQUENCE LISTING

The Sequence Listing associated with this application is provided in text format in lieu of a paper copy, and is hereby incorporated by reference into the specification. The name of the text file containing the Sequence Listing is 200187 462USPC SEQUENCE LISTING.txt. The text file is 7.1 KB, was created on Jan. 19, 2020, and is being submitted electronically via EFS-Web.

TECHNICAL FIELD

The present disclosure relates to a cAMP receptor protein variant, a microorganism including the same, and a method of producing an L-amino acid using the same.

BACKGROUND ART

CRP (cyclic AMP receptor protein), also called CAP (catabolite activator protein), is the most well-known transcription regulator inE. coli. CRP is characterized by having a carbon source-dependent regulating mechanism, which is represented by ‘catabolite repression’. This action is triggered by an intracellular concentration of cyclic AMP (hereinafter, referred to as ‘cAMP’). In the presence of a preferred carbon source such as glucose, the activity of adenylate cyclase is inhibited to lower cAMP, and this signal inhibits the expression of catabolic genes. In the opposite case, the activity of adenylate cyclase is increased, and as a result, repressors are suppressed and expression of catabolic genes is initiated. In addition, CRP is known to play various roles such as intracellular signal transduction through cAMP, osmotic regulation, responses to urgent situations of cells, biofilm generation, nitrogen fixation, iron transport, etc.

Reportedly, 418 genes ofE. coliare known to be regulated by CRP, but the corresponding mechanisms have not been yet clearly revealed (J Biol Eng. (2009) 24; 3:13). With such a wide range of regulatory abilities, CRP has the potential to show a variety of phenotypes by mutations. Because of its advantages, CRP has been studied as a target suitable for redesigning of strains at a cellular level, which are applicable to various environments. Recently, various experimentations have been conducted, such as a method of altering the expression of genes to be regulated by changing the degree of DNA binding by amino acid variation of CRP selected by bioinformatics (Nucleic Acids Research, (2009) 37: 2493-2503), a method of selectingE. coliresistant to heat, osmosis, and low temperature using an artificial transcription factor (ATF) prepared by fusion of a zinc finger DNA binding site and CRP ((Nucleic Acids Research, (2008) 36: e102), etc. In other words, since changes of the CRP expression promote a wide range of changes in expression of the downstream genes, CRP is likely to be a good tool for preparing microorganisms with useful traits.

DISCLOSURE

Technical Problem

The present inventors have developed a novel protein variant including one or more amino acid substitutions in an amino acid sequence of SEQ ID NO: 1, and they found that this protein variant may increase L-amino acid productivity, thereby completing the present disclosure.

Technical Solution

An object of the present disclosure is to provide a cAMP receptor protein variant.

Another object of the present disclosure is to provide a polynucleotide encoding the cAMP receptor protein variant.

Still another object of the present disclosure is to provide a vector including the polynucleotide.

Still another object of the present disclosure is to provide a microorganism of the genusEscherichiaincluding the variant.

Still another object of the present disclosure is to provide a method of producing an L-amino acid, the method including culturing the microorganism of the genusEscherichiain a medium.

Still another object of the present disclosure is to provide use of the variant or the microorganism of the genusEscherichiaincluding the variant in the production of L-amino acid.

Advantageous Effects

When a microorganism of the genusEscherichiaproducing an L-amino acid, the microorganism including a cAMP receptor protein variant of the present disclosure, is cultured, it is possible to produce the L-amino acid with a high yield. Accordingly, in industrial aspects, reduction of production costs, along with convenience of production, may be expected.

