CROSS REFERENCE TO RELATED APPLICATIONS AND CLAIM OF PRIORITY

This application claims benefit under 35 U.S.C. 119(e), 120, 121, or 365(c), and is a National Stage entry from International Application No. PCT/KR2017/013587, filed on Nov. 27, 2017, which claims priority to the benefit of Korean Patent Application No. 10-2016-0160302 filed in the Korean Intellectual Property Office on Nov. 29, 2016, the entire contents of which are incorporated herein by reference.

STATEMENT REGARDING GOVERNMENT SPONSORED APPLICATION

This application is financially supported by the 2016 Commercialization Project for Promising Technologies of the government of the Republic of Korea.

TECHNICAL FIELD

The present invention relates toLactobacillus reuteriCH53 strain having high productivity of 1,3-propanediol from glycerol and uses thereof.

BACKGROUND ART

As a major by-product of the current process for producing biodiesel, crude glycerol is generated in an amount which corresponds to about 10% (w/w) of the entire production. As the crude glycerol exhibits an influence on traditional market price of glycerol and cannot be directly released into an environment, it now becomes a critical environmental issue including processing costs. Thus, a method for conversion into an industrially valuable material like fuels and physiologically active materials by using low priced crude glycerol is actively developed.

According to fermentation by microorganism, glycerol can be converted to various chemical materials, and representative examples thereof include 1,3-propanediol. 1,3-Propanediol is used for various applications including fibers like highly functional clothing, carpet, and fabric for an automobile, plastic films, cosmetics, and the like. In particular, it is commercially available as “Propanediol”, which is a raw material of cosmetics, and it is known to have a more favorable moisturizing effect and anti-microbial effect than 1,3-butylene glycol which is used as a raw material of cosmetics in related art. At present moment, because 1,3-propanediol is mostly produced by using a recombinant microbial strain (Escherichia coli, Klepsiella, orClostridium), it has limitations of GMO, and there is high reluctant feel in the market about using it.

Under the circumstances, the inventors of the present invention made an effort to solve those problems, and, as a result, succeeded in newly isolatingLactobacillus reuteriCH53 strain, which is alactobacillusstrain safe for humans. Furthermore, based on the isolation ofLactobacillus reuteriCH53 strain, the inventors can provide a basis of a technique which enables production of 1,3-propanediol in very large amounts from glycerol or crude glycerol.

Meanwhile, in Korean Patent Application Publication No. 2010-0063585, “Mutant blocked in glycerol oxidation pathway for producing 1,3-propanediol” is disclosed, and, in Korean Patent Application Publication No. 2011-0129070, “Method for high-yield production of 3-hydroxypropionic acid from glycerol” is disclosed. However, nothing has been described with regard toLactobacillus reuteriCH53 strain having high productivity of 1,3-propanediol from glycerol and uses thereof as described in the present invention.

SUMMARY

The present invention is devised under the circumstances described in the above, and object of the present invention is to provideLactobacillus reuteriCH53 strain (KCTC13149BP) having high productivity of 1,3-propanediol, which is a key in the technique for producing, via microbial fermentation, 1,3-propandiol as a raw material of high value-added cosmetics (anti-microbial agent and moisturizing agent) by using crude glycerol as an industrial waste.

Lactobacillus reuteriCH53 strain (KCTC13149BP) can produce 1,3-propanediol in final amount of 16.3 g/L by consuming 20.1 g/L glycerol, and the production yield compared to glycerol is confirmed to be 0.81 g/g (1,3-propanediol/glycerol), which shows very high conversion rate of 98% when compared to the theoretical production yield (0.83 g/g). It was also confirmed that, according to optimized fed-batch culture, the production amount of 1,3-propanediol is 65.5 g/L (based on 54 hours) and the production per hour is 1.17 g/L/h.

At present moment, because 1,3-propanediol is mostly produced by using a recombinant microbial strain (Escherichia coli, Klepsiella, orClostridium), it has limitations of GMO and there is high reluctant feel in the market about using it. Accordingly, in the present invention,Lactobacillus reuteriCH53 strain, which is lactic acid bacterium, is newly isolated from a strain safe for humans (GRAS, Generally Recognized As Safe), and a production process technique for efficient production of 1,3-propanediol from crude glycerol by using the strain is provided.

To solve the problems that are described above, the present invention providesLactobacillus reuteriCH53 strain (Accession number: KCTC13149BP) having high productivity of 1,3-propanediol from glycerol.

Furthermore, the present invention provides a composition for producing 1,3-propanediol comprising the aforementioned strain or a culture solution thereof as an effective component.

Furthermore, the present invention provides a cosmetic composition for skin moisturization comprising the aforementioned strain or a culture solution thereof as an effective component.

Still furthermore, the present invention provides a method for producing 1,3-propanediol comprising culturing the aforementioned strain.

It is confirmed thatLactobacillus reuteriCH53 strain of the present invention has a very remarkable capability of converting glycerol or crude glycerol into 1,3-propanediol. Thus, sinceLactobacillus reuteriCH53 strain of the present invention, which is capable of producing large amounts of 1,3-propanediol, is one kind of lactic acid bacteria that are safe for humans (GRAS, Generally Recognized As Safe), when it is applied for producing 1,3-propanediol from crude glycerol, unlike existing GMO strains, it would be very advantageously used in related industries from the viewpoint of safety or environment and also providing an advantage of recycled use of resources.

