CROSS-REFERENCE TO RELATED APPLICATIONS

This application is based upon and claims the benefit of priority from Japanese Patent Application No. 2018-045901, filed Mar. 13, 2018, the entire contents of which are incorporated herein by reference.

FIELD

Embodiments described herein relate generally to a nucleic acid reaction tool, a nucleic acid detection/quantification kit, and a nucleic acid detection/quantification method.

BACKGROUND

Currently, genetic tests are carried out in various scenes such as clinical sites and criminal investigations along with the progress of genetic testing technology. The gene to be tested is detected or quantified by the real-time PCR method or LAMP method. For example, the real-time PCR method has high sensitivity and a wide quantification range, since it involves amplification of nucleic acid. In the LAMP method, a target gene can be detected and/or quantified without labeling with a fluorescent dye.

Genetic tests are often useful only when a plurality of target genes is detected and the results thereof are synthesized. For example, in the identification of pathogenic bacteria infecting patients carried out in a clinical site, it is preferable to examine a plurality of types of microorganisms suspected to be infected based on the symptoms of patients. In addition, in individual identification or the like carried out in a criminal investigation, a repetitive sequence at a plurality of gene loci is inspected to identify an individual comprehensively. Accordingly, an individual can be identified with high probability. Therefore, techniques for detecting a plurality of target genes have become very important.

Conventionally, when detecting a plurality of target genes, amplification reactions are carried out in different reaction vessels for each of the plurality of target genes to detect the presence or absence of each target gene. Alternatively, when the amplification reaction is carried out in one reaction vessel, a multi-nucleic acid amplification reaction is carried out in one reaction vessel containing reagents for amplifying all target genes. Detection of an amplification product is generally carried out by subjecting the amplification product to a DNA chip, electrophoresis or the like. Alternatively, amplification and detection of a target gene can also be carried out using a multi-nucleic acid reaction vessel in which a plurality of types of primer sets or a plurality of types of probe nucleic acids is fixed.

Under the circumstances described above, further development of a method for detecting and/or quantifying nucleic acid conveniently and highly sensitively is currently desired.

