This application is a U.S. National Stage of PCT/KR2017/009121, filed Aug. 22, 2017, which claims the benefit of Korean Patent Application Nos. 10-2016-0124951, filed on Sep. 28, 2016 and 10-2017-0105825, filed on Aug. 22, 2017 in the Korean Intellectual Property Office.

TECHNICAL FIELD

The technical field of the present exemplary embodiment relates to a biological sample separation device which is capable of concentrating a target material in a specific section and being manufactured at a low cost. The present invention corresponds to a result of a research project (development of a paper-based molecular sieving mechanism and a solution for acute disease) supported by National Research Foundation of Korea with funds of the government (the Ministry of Education) in 2016 (Project No. 1345256126).

BACKGROUND ART

The contents described in this section merely provide background information on the present exemplary embodiment but do not constitute the related art. Modern medicine aims not to simply extend the lifespan, but to prolong the healthy life. Therefore, a paradigm of the future medicine is changing to implement3P of preventive medicine, predictive medicine, and personalized medicine, rather than focusing on the therapeutic medicine. In order to specifically realize the paradigm, early identification and early treatment of diseases have become important means and to this end, studies on a biomarker are actively being carried out.

A biomarker refers to an indicator which identifies a normal or abnormal state or predicts a therapeutic reaction, or is objectively measurable. As the biomarker, nucleic acids such as DNA or RNA (gene), protein, lipid, metabolite, and a change of a pattern thereof are used. That is, not only a simple material such as blood glucose for diagnosis of diabetes but also genes such as BCR-ABL gene fusion of chronic myelogenous leukemia which is a therapeutic target of Gleevec are biomarkers which are actually used in the clinical fields.

DNA (deoxyribonucleic acid) is a genetic material in the nucleus and a gene is a place where chemical information determining a type of protein created by an organism is stored. Information configuring human body is identified by analyzing DNA and various DNA analysis techniques have been studied, developed, and utilized for prevention and treatment of disease. In order to analyze the diseases using DNA, a gene amplification technique called polymerase chain reaction (PCR) is used. According to the PCR, in order to use a single strand obtained by continuously separating a double helix of DNA as an original for creating a new double helix, heating and cooling are repeated with a heat-stable DNA polymerase. Therefore, the DNA is heated to be separated into two strands. When a short DNA called “primer” is added thereto to be cooled, the primer binds to the DNA. When an enzyme called DNA polymerase is added thereto, the primer part becomes a starting point to replicate the DNA. The DNA is doubled by one cycle of heating and cooling. When the cycle of heating and cooling is repeated dozens of times, DNA may be multiplied billions of times in approximately one hour.

Protein is a complex molecule made up of relatively simple molecules called amino acids, which are generally very large in molecular weight. There are about twenty kinds of amino acids which form the protein and the amino acids are bonded together by chemical bonds to create polypeptide. In this case, the bonds of amino acids are called peptide bonds and are referred to as a polypeptide in the sense that there are many (poly) peptide bonds. In a broad sense, a protein may also be called a polypeptide and generally speaking, when a molecular weight is relatively small, it is called a polypeptide and when a molecular weight is very large, it is called a protein. Such a protein is a representative molecule that constitutes a body of an organism and is a material which serves as a catalyst for various chemical reactions in cells and takes charge of immunity. The protein is a very important organic matter which constitutes a living organisms and participates in in vivo reactions and energy metabolism.

The DNA or the protein is analyzed to determine generation and progression of cancer or disease. Specifically, for the early diagnosis and treatment of intractable diseases such as cancers, a blood fingerprint analysis technique which finds an indicator protein showing a minute changes in an initial stage where a normal cell in the protein included in the blood is developed to a cancer cell is known. The blood fingerprint analysis is a technique which comprehensively analyzes mass spectrometric data of metabolites present in the blood of cancer patients to diagnose the cancer through changing patterns, by taking into account that metabolites of the human body can change depending on the presence or absence of cancer. The blood fingerprint analysis is advantageous in that the cancer is diagnosed immediately from the blood so that the information is promptly achieved.

However, since the current techniques and devices for protein analysis use nano technology, it is difficult to manufacture the devices and the devices are relatively expensive, so that it is difficult to propagate the devices. Further, there is disadvantages in that a high sensitive sensor is necessary for a protein analysis device or it is difficult to precisely analyze with a small amount of sample. In the meantime, recently, in accordance with the development of a nano technology and micro electro mechanical systems (MEMS) technique, it is possible to promptly separate and purify a necessary material only with a small amount of sample by patterning a single fluidics with a nano structure. Further, efforts are actively made to apply these techniques to biotechnology and medical engineering fields. Further, the developed MEMS/NEMS techniques allow the nano structure to be precisely patterned in a desired location with a several nanometers of error tolerances and these techniques are combined with a micro fluidic channel to be actively studied as micro total analysis system (m-TAS) or Lab-on-a-chip.

Specifically, a method which implements a biomolecule concentration and separation apparatus capable of improving a detection accuracy by concentrating biomolecules even in a small amount of sample using glass or other heavy and expensive material is known. According to this method, a film is formed so as to concentrate a target material in a predetermined location while diffusing a material to be analyzed through a narrow pipe or a thin plate. However, these methods have problems in that it is difficult to manufacture the concentration and separation device or it is costly and the device is large and heavy or inconvenient in handling.

DISCLOSURE

Technical Problem

A main object of the exemplary embodiments of the present invention is to provide a biological sample separation device which is manufactured at a low cost by applying an electric field to a selective ion permeable layer based on origami to concentrate and separate a target material in a specific area.

Another object of the exemplary embodiment of the present invention is to concentrate a target material (collection object) in a desired location and separate a non-target material (separation object) through a filter layer in which papers are compressed to adjust a size of micro pore.

Other and further objects of the present disclosure which are not specifically described can be further considered within the scope easily deduced from the following detailed description and the effect.

Technical Solution

According to an aspect of the present exemplary embodiment, a sample separation device includes: a base which is folded with a predetermined interval and includes a plurality of base units which serves as a basic unit to be folded, a coating layer which is located in at least a partial area of the base to prevent the adsorption of a sample to be processed and separate a storage space or a movement path, a plurality of reservoirs which has an area defined by the coating layer and located in each base unit and adsorbs and stores at least a part of a target material to be separated which is included in a sample to be measured or a separation target material excluding the target material from the sample or provides a movement path, and a selective ion permeable layer which at least partially overlaps at least some of reservoirs and selectively transmits ions.

According to another aspect of the present exemplary embodiment, a sample separation device includes: a base which includes a plurality of base units and is formed such that the plurality of base units is folded, a coating layer which is located in at least a partial area of the base to prevent the adsorption of a sample and separate a storage space or a movement path, a plurality of reservoirs which has an area set by the collating layer, is located in the plurality of base units, and stores or moves a collection object to be separated from the sample or a separation object excluding the collection object included in the sample, a selective ion permeable layer which is coupled to some reservoirs of the plurality of reservoirs to selectively transmit ions, and a filter layer which is located in the middle of a movement path of the collection object formed by folding the plurality of base units to filter the separation object.

Advantageous Effects

As described above, according to the exemplary embodiments of the present invention, an electric field is applied to a selective ion permeable layer based on origami to concentrate and separate a target material in a specific area to manufacture the device at a low cost.

According to the exemplary embodiments of the present invention, a target material is concentrated and separated in a desired location through a filter layer in which a paper are compressed to adjust a size of micro pore.

Even if the effects are not explicitly mentioned here, the effects described in the following specification which are expected by the technical features of the present disclosure and their potential effects are handled as described in the specification of the present disclosure.

