CROSS REFERENCE TO RELATED APPLICATION

This application claims the benefit under 35 U.S.C. 119(a)-(d) to Chinese Application No. 201611259507.4, filed Dec. 30, 2016, which application is hereby incorporated by reference in its entirety.

TECHNICAL FIELD

The present disclosure relates to the field of sequencing techniques and, more particularly, to a method for controlling base sequence determination, a base sequence determination system and a control device for controlling base sequence determination.

BACKGROUND

Sequence determination, i.e., sequencing, includes determination of nucleic acid sequences. Sequencing platforms currently available in the market include generations I, II, and III of sequencing platforms.

From the point of view of functional control, the sequencing instrument includes a detection module and utilizes the detection module to transform and/or collect varied information in the biochemical reactions in sequencing to determine the sequence. The detection module generally includes an optical detection module, a current detection module and a acid-base (pH) detection module. The sequencing platform based on the optical detection principle is used for sequence determination by analyzing variation in the optical signals collected from a sequencing biochemical reaction.

SUMMARY OF THE INVENTION

An embodiment of the present disclosure is intended to at least solve one of the technical problems present in the related art or at least provide an alternative practical solution. To this end, the embodiment of the present disclosure provides a method, a sequence determination system, and a control device for controlling the base sequence determination.

An embodiment of the present disclosure provides a method for controlling base sequence determination, wherein the base sequence determination includes a first biochemical reaction, a second biochemical reaction, and photographing, the first biochemical reaction and the second biochemical reaction are carried out on a reaction device, and a sequence determination system is configured to control the base sequence determination, the sequence determination system includes a fluid device and an optical device, the reaction device is connected to the fluid device; the reaction device includes a first component and a second component, a subject sample being placed on each of the first component and the second component; and, a repeated executable unit comprised in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing; wherein the method comprises, after completion of following initial steps, when one of the first component and the second component is subjected to the second biochemical reaction and the first biochemical reaction of the sample by using the fluid device, photographing the sample in the other component with the optical device, and wherein the initial steps include:

a. using the fluid device to perform the first biochemical reaction of the sample on one of the first component and the second component,

b. using the optical device to photograph the sample on the component after the first biochemical reaction, and

c. using the fluid device to perform the first biochemical reaction of the sample on another one of the first component and the second component.

In the above-described method, the reaction device is divided into at least two components, and one of the components is subjected to a biochemical reaction by the fluid device while another one of the components is photographed, i.e., has its image acquired by the optical means, thereby reducing the sequencing time and improving the sequencing efficiency.

An embodiment of the present disclosure provides a sequence determination system for controlling base sequence determination, wherein the base sequence determination comprises a first biochemical reaction, a second biochemical reaction, and photographing, wherein the first biochemical reaction and the second biochemical reaction take place on a reaction device, wherein the sequence determination system comprises a control device, a fluid device and an optical device, the reaction device being connected to the fluid device; the reaction device comprises a first component and a second component, a subject sample being placed on each of the first component and the second component; and, a repeated executable unit comprised in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing; the control device being configured to, after completion of following initial steps, when one of the first component and the second component is subjected to the second biochemical reaction and the first biochemical reaction of the sample by using the fluid device, photographing the sample in the other component with the optical device, and wherein the initial steps comprise:

a. utilizing the fluid device, by the control device, to perform the first biochemical reaction of the sample on one of the first component and the second component,

b. utilizing the optical device, by the control device, to photograph the sample on the component after the first biochemical reaction, and

c. utilizing the fluid device, by the control device, to perform the first biochemical reaction of the sample on another one of the first component and the second component.

In the above-described sequence determination system, when performing base sequence determination, the reaction device is divided into at least two components, and one of the components is subjected to a biochemical reaction by the fluid device while another one of the components is photographed, i.e., has its image acquired by the optical means, thereby reducing the sequencing time and improving the sequencing efficiency.

A control device for controlling base sequence determination for use in a sequence determination system according to an embodiment of the present disclosure is provided, the sequence determination system comprising a fluid device and an optical device, wherein the control device comprises: a storage device for storing data, the data comprising a computer executable program; and

a processor for executing the computer executable program, wherein the executing the computer executable program comprises performing the above-described method.

A computer-readable storage medium according to an embodiment of the present disclosure is provided for storing a computer executable program, executing the program comprising executing the above-described method. The computer-readable storage medium may include read-only memory, random access memory, magnetic disks, or optical disks.

Additional aspects and advantages of the embodiments of the present disclosure will be set forth in part in the description which follows, and in part will be apparent from the following description, or may be learned by practice of the embodiments of the disclosure.

