RELATED APPLICATIONS

This application claims priority to Taiwan Application Serial Number 109102297, filed Jan. 21, 2020, which is herein incorporated by reference.

BACKGROUND

Technical Field

The present disclosure relates to a sensor element and a fabricating method thereof. More particularly, the present disclosure relates to a non-enzyme sensor element and a fabricating method thereof.

Description of Related Art

In the field of clinical medicine, detecting the concentration of organic substances in the human body can be used to determine the signs of diseases and the state of the human body, and can be further applied in the fields of sports management and disease detection, etc.

Lactic acid is considered to be an important physiological index, but the conventional techniques for detecting lactic acid are very expensive, taking long time for detection, or sensitive to the environmental influence, which cannot satisfy the requirements for point-of-care medical detections or other fields. Furthermore, the conventional non-enzyme sensor detects the lactic acid in two ranges of 0.5 mM to 35 mM and 11.9 mM to 188 mM. However, the range of the lactic acid concentration in the human body fluid can be as wide as 0 mM to 60 mM. It shows that each conventional non-enzyme sensor alone is not suitable for human metabolites detection.

Therefore, it is important to develop a rapid and accurate non-enzyme sensor and accurate non-enzyme sensor element which is suitable for detecting human metabolites.

SUMMARY

According to one aspect of the present disclosure, a fabricating method of a non-enzyme sensor element includes a printing step, a coating step and an electroplating step. In the printing step, a conductive material is printed on a surface of a substrate to form a working electrode, a reference electrode and an auxiliary electrode, and a porous carbon material is printed on the working electrode to form a porous carbon layer. In the coating step, a graphene film material is coated on the porous carbon layer of the working electrode to form a graphene layer. In the electroplating step, a metal is electroplated on the graphene layer by a pulse constant current to form a catalyst layer including a metal oxide.

According to another aspect of the present disclosure, a non-enzyme sensor element fabricated by the fabricating method of the non-enzyme sensor element according to the aforementioned aspect and for detecting a concentration of an analyte includes the substrate and a conductive layer. The conductive layer is printed on the surface of the substrate and has the working electrode, the reference electrode and the auxiliary electrode, wherein the working electrode includes the porous carbon layer, the graphene layer and the catalyst layer. The porous carbon layer is connected to the conductive layer. The graphene layer is connected to the porous carbon layer. The catalyst layer is electroplated on the graphene layer, and the catalyst layer includes the metal oxide and is used to oxidize the analyte.

According to still another aspect of the present disclosure, a non-enzyme sensor for detecting a concentration of an analyte includes the non-enzyme sensor element according to the aforementioned aspect and a current sensor. The non-enzyme sensor element is used to oxidize the analyte to generate a current. The current sensor is electrically connected to the non-enzyme sensor element and converts the current to a concentration signal.

