of GFP10. The resulting PCR products were subcloned by Gibson assembly in pLVX-IRES-Puro. Constructs encoding the Epac-based cAMP sensor (GFP10-Epac-RlucII), RlucII-tagged Gα (αi1, αi2, αoA, αs), GFP10-Gγ1, GFP10-Gγ2, β-arrestin1-RlucII, β-arrestin2-RlucII, RlucII-Gγ2, human β2-adrenergic receptor (hβ2AR), and cmyc-hβ2AR-GFP10 were previously described (PMID: 15782186, PMID: 22534132, PMID: 23175530, PMID: 24309376, PMID: 19584306, PMID: 26658454, PMID: 16901982, PMID: 15155738). pCDNA3.1 ( + ) Gβ1 was bought at Missouri University of Science and Technology (cdna.org). Plasmids encoding for the following proteins were generously provided as follows: GRK6 and GRK2 by Dr Antonio De Blasi (Istituto Neurologico Mediterraneo Neuromed, Pozzilli, Italy), GRK5 by Dr Robert Lefkowitz (Duke University, Durham, NC). Kir3.2-GFP10 by Dr Terry Hebert (McGill University, Montréal, Canada). Kir3.1 subunit by Dr. Deborah J. Nelson (University of Chicago, Chicago, IL).

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HEK293be a criminal or something who is being looked for by the authorities.
The guy has dark hair, and I would go with black hair specifically.
The guy falls in love with a woman native to the country.
Said woman has blonde hair.
In one scene, the guy is in the metro when two undercover police agents approach him, identify themselves as such, and ask him for his papers (ID or passport, I guess). The guy acts as if he's reaching for his wallet and as soon as the train stops in the next station he shoves them away and escapes.
The guy's love interest has an ex-husband, and one day he (our guy) shows to her place unannounced and finds them having sex. In anger, he punches her ex and the guy