[Molecular diagnosis and prenatal diagnosis in a hereditary multiple osteochondromas family].
To identify the mutation in the disease gene and provide prenatal diagnosis for a hereditary multiple osteochondromas (HMO) family. The exons of EXT1 gene in the proband with HMO and his family members were amplified by PCR. The products were analyzed by direct sequencing. Prenatal genetic diagnosis was performed by amniocentesis sampling after genotyping the proband. In the family, the affected proband was heterozygous of the mutation of 1476_1477delTC in the EXT1 gene, and the proband's father carried the same mutation in part of his somatic cells. No mutation was found in the EXT1 gene of the proband's mother and other 11 siblings of his father. METHODS for molecular diagnosis and prenatal diagnosis of HMO were established and382 F.3d 447
UNITED STATES of America, Plaintiff-Appellee,v.Florence LOCKHART, a/k/a Florence Rebecca Lockhart, Defendant-Appellant.
No. 03-4815.
United States Court of Appeals, Fourth Circuit.
Argued: May 5, 2004.
Decided: September 8, 2004.

Appeal from the United States District Court for the Eastern District of Virginia, Richard L. Williams, J.


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ARGUED: Robert James Wagner, Assistant Federal Public Defender, Office of The Federal Public Defender, Richmond, Virginia, for Appellant. Brian D. Miller, Assistant United States Attorney, Office of The United States Attorney, Alexandria, Virginia, for Appellee.


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ON BRIEF: Frank W. Dunham, Jr., Federal Public Defender, Alexandria, Virginia, for Appellant. Paul J. McNulty, United States Attorney, Michael J. Elston, Assistant United States Attorney, Alexandria, Virginia; Michael C. Wallace, Sr., Assistant United States Attorney, Richmond, Virginia, for Appellee.


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Before WIDENER and GREGORY, Circuit Judges, and C. Arlen BEAM, Senior Circuit Judge of