at diagnosis, severity of onset (presence or absence of ketoacidosis), personal history of Hashimoto thyroiditis (HT), and family history of T1DM of at least one first-degree relative. Control group consisted of children and adolescents older than 10 years, staff of Papageorgiou General Hospital, and students of the Medical Faculty of Aristotle University of Thessaloniki. Before entering the study, an informed consent was obtained by adult participants and parents or guardians of individuals younger than 18 years. The research protocol was declared at the service of ClinicalTrials.gov and approved by the Ethics Committee of Faculty of Medicine of Aristotle University of Thessaloniki. The study was conducted according to the criteria of the Declaration of Helsinki.

The method includes four stages: (a) genomic isolation of deoxyribonucleic acid (DNA), (b) polymerase chain0 of 50

When you discuss trios that are currently in the NBA, you can't leave out the Miami Heat and Boston Celtics.

Miami is led by superstars and 20-somethings LeBron James, Dwyane Wade and Chris Bosh. And Boston features veterans Kevin Garnett, Paul Pierce and Ray Allen, although point guard Rajon Rondo really makes it the "Fantastic Four" instead of the "Big Three."

This slideshow will cover not only the league's best trios of the present, but the greatest of all time.

In order to make this list, the trio has to have led their respective team to at least one playoff appearance.

And in addition, it's all about three stars, instead of two superstars and a role player.

With that said, don't look for the Los Angeles Lakers' Shaquille O'Neal, Kobe Bryant