ER-Tracker dye and BODIPY-brefeldin A differentiate the endoplasmic reticulum and golgi bodies from the tubular-vacuole system in living hyphae of Pisolithus tinctorius.
Two fluorochromes, ER-TrackerTM Blue-White DPX dye and the fluorescent brefeldin A (BFA) derivative, BODIPY-BFA, label the endoplasmic reticulum (ER) in hyphal tips of Pisolithus tinctorius and allow its differentiation from the tubular-vacuole system at the light microscope level in living cells. The ER-Tracker dye labels a reticulate network similar in distribution to ER as seen in electron micrographs of freeze-substituted hyphae. BODIPY-BFA stains a thicker axially aligned structure with an expanded region at the apex, which is similar to that seen when hyphae are stained with ER-Tracker dye in the presence of unconjugated BFA. This structure is considered to be ER modified by BFA, a view supportedto the company and raises his wine, his eyes alight with mischief.

"Ah, Gelir," I hear. His friends laugh and call, "Sit down, there."

Yet he is determined. My lord's man remains steadfast and backs away from playful hands that seek to cuff him or pull him to sitting. Those Rangers elder to his years smile and lean in one tother to whisper and exchange a meaningful glance. It seems they know him well and are not surprised.

"Nay, nay!" he says, laughing and protecting his cup. "Is this not a wedding feast? Forgive me, my lord, I could not tell."

My lord says naught, but he attends. His brow rises and his look is gently amused, and by that his man takes heart.

"Does there not seem to be one thing yet