Soviet stage.

Rise of the Miners

On 19 September, 2008, FC Shakhter, alongside FC Vostok, were disqualified from the Kazakhstan Premier League for playing a fixed match. On 2 October, the authorities revised their decision and FC Shakhter were instead deducted nine points, while FC Vostok’s expulsion was upheld. Despite this setback, Shakhter have reemerged with a functional side based on the strong tactical prowess of Viktor Kumykov, whose temporal managerial career is spatially similar to the pastoral traditions of Kazakh nomadism, and whose promise to give up a 35-year smoking career should Shakhter progress underlies his loyalty to Karaganda’s Champions League ambitions.

Unlike the other ‘Shakhters’ of the post-Soviet space, namely Donetsk and Belarus’s Soligorsk, and with particular regard to the former, Karagandy are not a financially-constructed entity, which makesGroup 5: saline (negative control). Methyl cellulose was used as the thickening agent for all the groups and the medicament was packed inside the root canal. The canals were then sealed at both the ends with paraffin wax and incubated at 37ºC in an anerobic environment. After 5 days, the harvesting of dentin was carried out at 400 μm depth with a sterile Gates-Gliden drill no. 5 \[[@R28]\].

Bacterial Genomic DNA Isolation from Harvested Dentin
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To the dentin sample, 1ml of lysozyme stock solution was added and vortexed, which was then incubated at 37ºC for 30 minutes. To this mixture, 10% sodium dodecylesulphate (SDS) was added and vortexed. The samples were again incubated at 37ºC for 30 min in a water bath. Following the above step, equal volume of Phenol