in three stages. The first step was involved preparing and sterilizing the equipment and materials as well as the constructing required culture media. The second stage was involved sampling, the cultivation and identify the bacterial agents. In order to sampling from swabs dipped in the trypticase soy broth medium (TSB) or sterile saline was used, after pulling on surfaces of book, swap with flame entered into the tube containing transitional medium of TSB. At the end of sampling, the transitional medium was transferred to the laboratory and was placed for 24 h at 37°C temperature. Then, it was examined in term of bacteria growth, and also it cultured in media blood agar and Merck-Eosin Methylene Blue agar linearly, and then the plates were incubated at 37°C temperature forNow, Mumbai Metro hits logo hurdle

On the verge of being opened to the public, the Mumbai Metro has run into an unforeseen mega block over the logo to be displayed on the trains and at the stations.

While the Mumbai Metropolitan Region Development Authority (MMRDA) officials want the logo ‘Mumbai Metro logo’, Reliance Infrastructure authorities, which holds 76 per cent stake in the Mumbai Metro One Private Limited (MMOPL), say they are still awaiting this new logo design. Earlier, a ‘Reliance Metro’ logo was stuck on the trains and the stations.

These old logos have not been removed yet. The first phase of the metro rail is slated to opened anytome before the elections.

Sunday mid-day visited several stations and found the Reliance Metro logo at many stations such as Versova