far west as the Tioga–Broome County line even though NY 188 had continued to Owego. Instead, the state planned on transferring ownership and maintenance of former NY 17 from Owego east to the county line to Tioga County. This transaction was completed on November 24, 1969.

Designation and extensions
NY 434 was officially assigned on January 1, 1970, as part of a wide-scale update of NYSDOT's Official Description of Touring Routes in New York State. A list of changes accompanying the log described NY 434 as "a new number assigned to replace former NY 17 between the Broome–Tioga County line and NY 26 in Vestal." At the time, NY 434 also ended at NY 17 in Vestal. Although the STE was open from Vestal to Johnson City, it was not slated to become part of NY 17 until the segment ofadded in order to degrade free RNA into shorter chains that could be separated by gel permeation chromatography, while encapsulated RNA was protected from hydrolysis. After incubation, the mixture was put through a Sephadex G-50 column to separate vesicles with encapsulated RNA from the hydrolyzed RNA products. The amount of encapsulated RNA in the vesicle fraction was measured using a NanoDrop instrument set to the RNA mode in which the absorbance 260 and 280 nm is used to calculate the amount present. This amount was compared to the total RNA that was originally added and expressed as percent encapsulated. A control was also run in which the RNA was added to the lipid vesicles without being dried, then hydrolyzed with RNase before being put through the Sephadex column.