methods
=====================

Animal model
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The experimental protocols of the present study were approved by the Institutional Animal Care and Use Committee (IACUC) of the Chinese People's Liberation Army General Hospital and performed in accordance with the regulations for animal experiments defined by the ethics committee of The Chinese People's Liberation Army General Hospital. In total, 40 healthy, male Chinese Guangxi Bama miniature pigs were housed individually in a windowed room at 26±1°C, with a relative humidity of 40--50%. All the animals were appropriately acclimatized and observed for a period of 1 week. Food was withdrawn the evening prior to the experiment. The pigs were anesthetized by an intra-muscular injection of pentobarbital sodium (30 mg/kg) and 1,000 ml Ringer's solution was intravenously administered via the vena auricularis magna during the experiment. Athe country, it’s JOB ONE!”.

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About the Author

After spending several years touring the country as a stand up comedian, Ed Brayton tired of explaining his jokes to small groups of dazed illiterates and turned to writing as the most common outlet for the voices in his head. Read More...