of Soil Harmony who, in effect, is an agronomist, taking monthly soil samples as a health check, so nutrient levels and any deficiencies can be monitored.

He also says there's genuinely pretty good camaraderie among Premier League groundsmen. "We are often in touch with each other, swapping tips and even getting a look at each other's pitches," he says. "When the Saints play Arsenal at the Emirates next, Steve Braddock is going to show us around the club's London Colney training set-up and, afterwards, Paul Ashcroft will show us the stadium."

He'd had to miss the opening ceremony of the Markus Liebherr Pavilion at the beginning of November. He was in Madrid after arranging a visit with Real's head groundsman, Paul Burgess, to view the Spanish club's training facilities and,to form shuffled library members in vivo.
In one embodiment, the first plurality of selected library members is cloned or amplified on episomally replicable vectors, a multiplicity of said vectors is transferred into a cell and homologously recombined to form shuffled library members in vivo.
In one embodiment, the first plurality of selected library members is not fragmented, but is cloned or amplified on an episomally replicable vector as a direct repeat or indirect (or inverted) repeat, which each repeat comprising a distinct species of selected library member sequence, said vector is transferred into a cell and homologously recombined by intra-vector or inter-vector recombination to form shuffled library members in vivo.
In an embodiment, combinations of in vitro and in vivo shuffling are provided to enhance combinatorial diversity.
The present invention provides