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Dr. Mario appears in Dr. Mario World. Unlike the previous installments, he and Luigi are no longer the sole playable doctors; instead, they are joined by a plethora of their allies, such as Princess Peach and Yoshi, and even some enemies like Bowser, Bowser Jr., and the Koopalings.

Dr. Mario's special ability allows him to use a big Megavitamin with an "M" on it, which eliminates the lowest line. In Duel mode, he has average power and his attack gauge fills quickly, and his ability still affects everything positioned in the lowest line (two lines at higher levels). However, he has sub-par defense: he has a 30% chance of defending himself from level 1 attacks, but it decreases by 5% for each higher level.

In a version update, bothUnlike in his home series, Dr. Mario's design is somewhat modified for his appearance in Super Smash Bros. Melee: he has a blue stethoscope, a brown head mirror headband and, most noticeable, a pair of dark gray pants. Like his normal self, Dr. Mario's weight is 100 units.

According to Super Smash Bros. series creator Masahiro Sakurai, the concept of a slower, yet stronger Mario was originally considered to be used for Wario. Due to time constraints, however, this concept was instead given to Dr. Mario.[4] On a lesser note, some of Dr. Mario's animations are less energetic and thus make him appear more serious and contemplative, which Sakurai jokingly said "make him seem like an old man."[5]

Due to being a moveset clone, the overwhelming majority of Dr. Mario's