Rare fossilised footprints of Tasmanian tigers and devils, as well as those of giant megafauna and flightless birds, have been uncovered by scientists on Kangaroo Island, south of Adelaide.

Top: Tasmanian devil and print; middle left: extinct short-faced kangaroo and its prints; next: sooty oystercatcher; middle right: eastern quoll, bottom left: diprotodon optatum: middle: grey kangaroo and right: trackways from the KI site showing large kangaroo and diprotodontid footprints. ( Supplied: A. Camens/Peter Trusler/Dave Watts )

The South Australian site is on an isolated beach on Dudley Peninsula, and was found during efforts to survey 6,500 kilometres of Australia's coastline from Western Australia through South Australia and Victoria.

Dr Aaron Camens, from Flinders University, said more than 300 trace fossils showed the island's reputation for diverse wildlife extended as far backthese animals.

"They can show us elements of the animals' behaviour as well — how they interacted with each other and what kind of environments they moved through."

The Kangaroo Island site has evidence of reptiles, birds, mammals and invertebrates, potentially spanning back between 20,000 and 200,000 years, including time when the area became separated from the mainland.

Tasmanian tigers 'definitely extinct'

Dr Camens said there was no chance the Tasmanian tiger might still be prowling parts of the mainland or Kangaroo Island.

"If we've got thylacines still living in South Australia, we need a viable population and not just a single one," he said.

"For the tens of thousands of purported sightings across Australia, we don't have concrete evidence of a single one anywhere on the mainland."

Kangaroo Island residents drew the area to