A chapel at Neilston was first recorded in 1163 in a charter of Paisley Abbey.
It was commissioned by Sir Robert Croc, as part of a feudal requirement by the High Steward of Scotland, Walter fitz Alan.
The original Neilston Kirk was one storey high, and was rebuilt in 1762 to accommodate the growing population of the parish.
The only remaining parts of the original building are a Gothic window in a back wall and the burial vault of the Mure family of Caldwell, including the tomb of Laird, scholar and MP for Renfrewshire, William Mure (1799–1860).
Between 1796 and 1798 the roof was taken off the church and an additional storey constructed, making space for a gallery to accommodate the growing population of Neilston.
The structure has a spire, a clock, and 940 sittings.
The old graveyard is centuries old and has a headstone dating from the 15th century.
The church is part of the Church of Scotland, and has a Category B listing from Historic Environment Scotland.

In 1559, in the Scottish Reformation, an image of Mary mother of Jesus was taken from Neilston Parish Church and thrown into a pool of the River Levern.
The pool ever since has been known as the Midge Hole.
One of Neilston Parish Church's most celebrated ministers was Dr Alexander Fleming and his Life (1883) contains much of interest relating to Neilston.
In 1826, despite enlargement of the church, it was too small to accommodate the population and the Heritors demanded payment of seat rents for those attending services.
Dr Fleming proceeded to preach from a tent erected in the graveyard for a period of about eight years, insisting that "the people of the Parish are entitled to hear the gospel without money or price."
The case went to the House of Lords and although it was not successful, the parishioners were subsequently able to return to the church and take their places without paying rent and with no further opposition from the Heritors.
In 2003, in a major refurbishment, six skeletons were found beneath the floorboards of the church.
Initially sealed off as a crime scene, archeologists from the University of Glasgow confirmed the skeletons were around 400 years old.
A local historian suggested they could be the bones of former priests.

Crofthead Mill in Neilston is a Category B listed building.
It is Neilston's largest and oldest cotton mill, dating in part from 1792 but predominantly 1880 after much of the original building was destroyed by fire.
The mill is now used by J & M Murdoch & Son Ltd., a transport, waste disposal and recycling company.
Nether Kirkton House is a mansion and the former home of whisky heiress Marion Buchanan.
It was Category B listed but had this status removed in 2017.
A chapel at Neilston was first recorded in 1163 in a charter of Paisley Abbey. It was commissioned by Sir Robert Croc, as part of a feudal requirement by the High Steward of Scotland, Walter fitz Alan. The original Neilston Kirk was one storey high, and was rebuilt in 1762 to accommodate the growing population of the parish. The only remaining parts of the original building are a Gothic window in a back wall and the burial vault of the Mure family of Caldwell, including the tomb of Laird, scholar and MP for Renfrewshire, William Mure (1799–1860). Between 1796 and 1798 the roof was taken off the church and an additional storey constructed, making space for a gallery to accommodate the growing population of Neilston. The structure has a spire, a clock, and 940 sittings. The old graveyard is centuries old and has a headstone dating from the 15th century. The church is part of the Church of Scotland, and has a Category B listing from Historic Environment Scotland.
In 1559, in the Scottish Reformation, an image of Mary mother of Jesus was taken from Neilston Parish Church and thrown into a pool of the River Levern. The pool ever since has been known as the Midge Hole. One of Neilston Parish Church's most celebrated ministers was Dr Alexander Fleming and his Life (1883) contains much of interest relating to Neilston. In 1826, despite enlargement of the church, it was too small to accommodate the population and the Heritors demanded payment of seat rents for those attending services. Dr Fleming proceeded to preach from a tent erected in the graveyard for a period of about eight years, insisting that "the people of the Parish are entitled to hear the gospel without money or price." The case went to the House of Lords and although it was not successful, the parishioners were subsequently able to return to the church and take their places without paying rent and with no further opposition from the Heritors. In 2003, in a major refurbishment, six skeletons were found beneath the floorboards of the church. Initially sealed off as a crime scene, archeologists from the University of Glasgow confirmed the skeletons were around 400 years old. A local historian suggested they could be the bones of former priests.
Crofthead Mill in Neilston is a Category B listed building. It is Neilston's largest and oldest cotton mill, dating in part from 1792 but predominantly 1880 after much of the original building was destroyed by fire. The mill is now used by J & M Murdoch & Son Ltd., a transport, waste disposal and recycling company. Nether Kirkton House is a mansion and the former home of whisky heiress Marion Buchanan. It was Category B listed but had this status removed in 2017.
Neilston