The following NOCs had qualified riders to compete in the road race event.
The following riders were confirmed by their respective NOCs.

* ref:
Over time limit (OTL)
Under UCI regulations for one-day road races (article 2.3.039), "Any rider finishing in a time exceeding that of the winner by more than 5% shall not be placed".
The following NOCs had qualified riders to compete in the road race event. The following riders were confirmed by their respective NOCs.
* ref:
Over time limit (OTL)
Under UCI regulations for one-day road races (article 2.3.039), "Any rider finishing in a time exceeding that of the winner by more than 5% shall not be placed".
Cycling at the 2016 Summer Olympics – Women's individual road race