In the autumn of 1495, when an epidemic of measles struck Touraine, Charles VIII (who after returning from Italy remained in Lyon, where he was joined by the Queen), ordered the child to be even more closely cloistered in Amboise.
But to no avail: Charles Orlando contracted measles, and in spite of the efforts of the doctors and the prayers of the monks, he died on 16 December 1495.

Charles VIII, deeply affected, but advised by his physicians to remain staunch and cheerful, succeeded in hiding his sorrow; Anne gave herself up to her grief so violently that for a time her life, and her sanity, were feared for.
The year following his death, a younger brother was born and he, too, was named Charles, but he only lived for less than a month (8 September 1496 – 2 October 1496).
Both children were buried at Saint Gatien's Cathedral, Tours, with gisants erected for them by the French sculptor Michel Colombe in 1506.
In the autumn of 1495, when an epidemic of measles struck Touraine, Charles VIII (who after returning from Italy remained in Lyon, where he was joined by the Queen), ordered the child to be even more closely cloistered in Amboise. But to no avail: Charles Orlando contracted measles, and in spite of the efforts of the doctors and the prayers of the monks, he died on 16 December 1495.
Charles VIII, deeply affected, but advised by his physicians to remain staunch and cheerful, succeeded in hiding his sorrow; Anne gave herself up to her grief so violently that for a time her life, and her sanity, were feared for. The year following his death, a younger brother was born and he, too, was named Charles, but he only lived for less than a month (8 September 1496 – 2 October 1496). Both children were buried at Saint Gatien's Cathedral, Tours, with gisants erected for them by the French sculptor Michel Colombe in 1506.
Charles Orlando, Dauphin of France