Chorismate synthase (CS) catalyzes the last step in the shikimate pathway—the formation of chorismate.
Two classes of CS are known, both of which require FMN, but are divided on their need for NADPH as a reducing agent.
The proposed mechanism for CS involves radical species.
The radical flavin species has not been detected spectroscopically without using a substrate analogue, which suggests that it is short-lived.
However, when using a fluorinated substrate, a neutral flavin semiquinone was detected.
Chorismate synthase (CS) catalyzes the last step in the shikimate pathway—the formation of chorismate. Two classes of CS are known, both of which require FMN, but are divided on their need for NADPH as a reducing agent. The proposed mechanism for CS involves radical species. The radical flavin species has not been detected spectroscopically without using a substrate analogue, which suggests that it is short-lived. However, when using a fluorinated substrate, a neutral flavin semiquinone was detected.
Flavin adenine dinucleotide