Pauline Therese was born in Riga, one of the five children of Duke Louis of Württemberg and his wife, Princess Henriette of Nassau-Weilburg.
Her siblings included Maria Dorothea, Archduchess of Austria; Amelia, Duchess of Saxe-Hildburghausen; Elisabeth Alexandrine, Princess of Baden, and Duke Alexander of Württemberg himself the founder of the Teck branch of the family.

Her paternal grandparents were Frederick II Eugene, Duke of Württemberg, and Friederike Dorothea of Brandenburg-Schwedt.
Her maternal grandparents were Charles Christian, Prince of Nassau-Weilburg, and Princess Carolina of Orange-Nassau, a daughter of William IV, Prince of Orange.
Pauline Therese was born in Riga, one of the five children of Duke Louis of Württemberg and his wife, Princess Henriette of Nassau-Weilburg. Her siblings included Maria Dorothea, Archduchess of Austria; Amelia, Duchess of Saxe-Hildburghausen; Elisabeth Alexandrine, Princess of Baden, and Duke Alexander of Württemberg himself the founder of the Teck branch of the family.
Her paternal grandparents were Frederick II Eugene, Duke of Württemberg, and Friederike Dorothea of Brandenburg-Schwedt. Her maternal grandparents were Charles Christian, Prince of Nassau-Weilburg, and Princess Carolina of Orange-Nassau, a daughter of William IV, Prince of Orange.
Pauline Therese of Württemberg