The family contains more than 4,000 species classified under 75 genera.
Recently, a comprehensive phylogenetic classification of the genera based on both molecular and morphological characters has been published.

Subfamily Drosophilinae Rondani, 1856:
Tribe Colocasiomyini Okada, 1989:
Genus Baeodrosophila Wheeler & Takada, 1964
Genus Balara Bock, 1982
Genus Chymomyza Czerny, 1903
Genus Colocasiomyia de Meijere, 1914
Genus Lissocephala Malloch, 1929
Genus Neotanygastrella Duda, 1925
Genus Phorticella Duda, 1924
Genus Scaptodrosophila Duda, 1923
Genus Protochymomyza Grimaldi, 1987
Tribe Drosophilini Okada, 1989:
Genus Arengomyia Yafuso & Toda, 2008
Genus Bialba Bock, 1989
Genus Calodrosophila Wheeler & Takada, 1964
Genus Celidosoma Hardy, 1965
Genus Collessia Bock, 1982
Genus Dettopsomyia Lamb, 1914
Genus Dichaetophora Duda, 1940
Genus Dicladochaeta Malloch, 1932
Genus Drosophila Fallén, 1823
Genus Hirtodrosophila Duda, 1923
Genus Hypselothyrea Okada, 1956
Genus Idiomyia Grimshaw, 1901 (Hawaiian Drosophila)
Genus Jeannelopsis Séguy, 1938
Genus Laccodrosophila Duda, 1927
Genus Liodrosophila Duda, 1922
Genus Lordiphosa Basden, 1961
Genus Microdrosophila Malloch, 1921
Genus Miomyia Grimaldi, 1987
Genus Mulgravea Bock, 1982
Genus Mycodrosophila Oldenberg, 1914
Genus Palmomyia Grimaldi, 2003
Genus Paraliodrosophila Duda, 1925
Genus Paramycodrosophila Duda, 1924
Genus Poliocephala Bock, 1989
Genus Samoaia Malloch, 1934
Genus Scaptomyza Hardy, 1849
Genus Sphaerogastrella Duda, 1922
Genus Styloptera Duda, 1924
Genus Tambourella Wheeler, 1957
Genus Zaprionus Coquillett, 1902
Genus Zaropunis Tsacas, 1990
Genus Zapriothrica Wheeler, 1956
Genus Zygothrica Wiedemann, 1830
Incertae sedis:
Genus Marquesia Malloch, 1932
Subfamily Steganinae Hendel, 1917:
Tribe Gitonini Grimaldi, 1990:
Genus Allopygaea Tsacas, 2000
Genus Acletoxenus Frauenfeld, 1868
Genus Amiota Loew, 1862
Genus Apenthecia Tsacas, 1983
Genus Apsiphortica Okada, 1971
Genus Cacoxenus Loew, 1858
Genus Crincosia Bock, 1982
Genus Electrophortica Hennig, 1965
Genus Erima Kertész, 1899
Genus Gitona Meigen, 1830
Genus Hyalistata Wheeler, 1960
Genus Luzonimyia Malloch, 1926
Genus Mayagueza Wheeler, 1960
Genus Paracacoxenus Hardy & Wheeler, 1960
Genus Paraleucophenga Hendel, 1914
Genus Paraphortica Duda, 1934
Genus Phortica Schiner, 1862
Genus Pseudiastata Coquillett, 1901
Genus Pseudocacoxenus Duda, 1925
Genus Rhinoleucophenga Hendel, 1917
Genus Soederbomia Hendel, 1938
Genus Trachyleucophenga Hendel, 1917
Tribe Steganini Okada, 1989:
Genus Eostegana Hendel, 1913
Genus Leucophenga Mik, 1866
Genus Pararhinoleucophenga Duda, 1924
Genus Parastegana Okada, 1971
Genus Pseudostegana Okada, 1978
Genus Stegana Meigen, 1830
Incertae sedis:
Genus Neorhinoleucophenga Duda, 1924
Genus Pyrgometopa Kertész, 1901
The family contains more than 4,000 species classified under 75 genera. Recently, a comprehensive phylogenetic classification of the genera based on both molecular and morphological characters has been published.
Subfamily Drosophilinae Rondani, 1856:
Tribe Colocasiomyini Okada, 1989:
Genus Baeodrosophila Wheeler & Takada, 1964
Genus Balara Bock, 1982
Genus Chymomyza Czerny, 1903
Genus Colocasiomyia de Meijere, 1914
Genus Lissocephala Malloch, 1929
Genus Neotanygastrella Duda, 1925
Genus Phorticella Duda, 1924
Genus Scaptodrosophila Duda, 1923
Genus Protochymomyza Grimaldi, 1987
Tribe Drosophilini Okada, 1989:
Genus Arengomyia Yafuso & Toda, 2008
Genus Bialba Bock, 1989
Genus Calodrosophila Wheeler & Takada, 1964
Genus Celidosoma Hardy, 1965
Genus Collessia Bock, 1982
Genus Dettopsomyia Lamb, 1914
Genus Dichaetophora Duda, 1940
Genus Dicladochaeta Malloch, 1932
Genus Drosophila Fallén, 1823
Genus Hirtodrosophila Duda, 1923
Genus Hypselothyrea Okada, 1956
Genus Idiomyia Grimshaw, 1901 (Hawaiian Drosophila)
Genus Jeannelopsis Séguy, 1938
Genus Laccodrosophila Duda, 1927
Genus Liodrosophila Duda, 1922
Genus Lordiphosa Basden, 1961
Genus Microdrosophila Malloch, 1921
Genus Miomyia Grimaldi, 1987
Genus Mulgravea Bock, 1982
Genus Mycodrosophila Oldenberg, 1914
Genus Palmomyia Grimaldi, 2003
Genus Paraliodrosophila Duda, 1925
Genus Paramycodrosophila Duda, 1924
Genus Poliocephala Bock, 1989
Genus Samoaia Malloch, 1934
Genus Scaptomyza Hardy, 1849
Genus Sphaerogastrella Duda, 1922
Genus Styloptera Duda, 1924
Genus Tambourella Wheeler, 1957
Genus Zaprionus Coquillett, 1902
Genus Zaropunis Tsacas, 1990
Genus Zapriothrica Wheeler, 1956
Genus Zygothrica Wiedemann, 1830
Incertae sedis:
Genus Marquesia Malloch, 1932
Subfamily Steganinae Hendel, 1917:
Tribe Gitonini Grimaldi, 1990:
Genus Allopygaea Tsacas, 2000
Genus Acletoxenus Frauenfeld, 1868
Genus Amiota Loew, 1862
Genus Apenthecia Tsacas, 1983
Genus Apsiphortica Okada, 1971
Genus Cacoxenus Loew, 1858
Genus Crincosia Bock, 1982
Genus Electrophortica Hennig, 1965
Genus Erima Kertész, 1899
Genus Gitona Meigen, 1830
Genus Hyalistata Wheeler, 1960
Genus Luzonimyia Malloch, 1926
Genus Mayagueza Wheeler, 1960
Genus Paracacoxenus Hardy & Wheeler, 1960
Genus Paraleucophenga Hendel, 1914
Genus Paraphortica Duda, 1934
Genus Phortica Schiner, 1862
Genus Pseudiastata Coquillett, 1901
Genus Pseudocacoxenus Duda, 1925
Genus Rhinoleucophenga Hendel, 1917
Genus Soederbomia Hendel, 1938
Genus Trachyleucophenga Hendel, 1917
Tribe Steganini Okada, 1989:
Genus Eostegana Hendel, 1913
Genus Leucophenga Mik, 1866
Genus Pararhinoleucophenga Duda, 1924
Genus Parastegana Okada, 1971
Genus Pseudostegana Okada, 1978
Genus Stegana Meigen, 1830
Incertae sedis:
Genus Neorhinoleucophenga Duda, 1924
Genus Pyrgometopa Kertész, 1901
Drosophilidae