On 13 April 2001, Comfort appeared at the 27th National Convention of American Atheists in Orlando, Florida, where he debated Ron Barrier, the National Spokesperson for American Atheists.
Comfort later stated that "they laughed at my humor, and although there was unified mockery at some of the things that I said, I was able to go through the Ten Commandments, the fact of Judgment Day, the reality of Hell, the Cross, and the necessity of repentance, and no one stopped me."

On 5 May 2007, Comfort and Cameron participated in a televised debate with Brian Sapient and Kelly O'Connor of the Rational Response Squad, at Calvary Baptist Church in Manhattan.
The debate focused on the existence of God, which Comfort claimed he could prove scientifically without relying on the Bible.
However during the debate, Cameron and Comfort repeatedly referenced the Ten Commandments and denied the theory of evolution.

Nightline correspondent Martin Bashir moderated the 2007 event.

In February 2009, Comfort challenged evolutionary biologist Richard Dawkins to a debate, offering to donate $10,000 to him.
Dawkins, who had previously stated a general policy not to debate with creationists, said he would agree to do it if Comfort made a $100,000 donation to the Richard Dawkins Foundation for Reason and Science non-profit organization.
Comfort raised his offer to $20,000, which according to biologist PZ Myers was "not enough".
On 13 April 2001, Comfort appeared at the 27th National Convention of American Atheists in Orlando, Florida, where he debated Ron Barrier, the National Spokesperson for American Atheists. Comfort later stated that "they laughed at my humor, and although there was unified mockery at some of the things that I said, I was able to go through the Ten Commandments, the fact of Judgment Day, the reality of Hell, the Cross, and the necessity of repentance, and no one stopped me."
On 5 May 2007, Comfort and Cameron participated in a televised debate with Brian Sapient and Kelly O'Connor of the Rational Response Squad, at Calvary Baptist Church in Manhattan. The debate focused on the existence of God, which Comfort claimed he could prove scientifically without relying on the Bible. However during the debate, Cameron and Comfort repeatedly referenced the Ten Commandments and denied the theory of evolution.
Nightline correspondent Martin Bashir moderated the 2007 event.
In February 2009, Comfort challenged evolutionary biologist Richard Dawkins to a debate, offering to donate $10,000 to him. Dawkins, who had previously stated a general policy not to debate with creationists, said he would agree to do it if Comfort made a $100,000 donation to the Richard Dawkins Foundation for Reason and Science non-profit organization. Comfort raised his offer to $20,000, which according to biologist PZ Myers was "not enough".
Ray Comfort