The first car to be built, 1001, was delivered on 16 April 1962 from Tokyo Kisha's factory in Kōtō, Tokyo, and transferred by road and rail to Nippon Sharyo's Warabi factory in Kawaguchi, Saitama on 17 April.
The two-car set A was then unveiled to the press at Nippon Sharyo on 25 April 1962.

Test running was performed on the 32 km "model track" test section between Kamonomiya in Odawara and Ayase in Kanagawa prefecture from 26 June 1962.
A speed of 190 km/h (118 mph) was first recorded on 27 October 1962 by set B, breaking the previous record of 175 km/h (109 mph) set by the narrow-gauge (1,067 mm (3 ft 6 in)) KuMoYa 93 test train on 21 November 1960.
A speed of 200 km/h (124 mph) was first recorded on 31 October 1962 by set B, 210 km/h (130 mph) was reached on 20 December 1962, 243 km/h (151 mph) was recorded on 19 March 1963, and on 30 March 1963, set B recorded a world speed record of 256 km/h (159 mph).

With the start of test-running  and trial service on the Tōkaidō Shinkansen between Tokyo and Osaka in June 1964, set A was reclassified as a Class 941 emergency relief train (cars 941-1 and 941-2), and set B as a Class 922 track and overhead wire inspection train (set T1) (cars 922-1–4).
The Class 941 remained out of use at Osaka Depot, and the Class 922 set was active until the new Class 922/10 set, T2, was delivered in 1974.

All six prototype cars were cut up at Hamamatsu Works between 1975 and 1976, being used to test the cutting-up facilities ahead of the first batch of 360 0 series cars due for withdrawal.
The first car to be built, 1001, was delivered on 16 April 1962 from Tokyo Kisha's factory in Kōtō, Tokyo, and transferred by road and rail to Nippon Sharyo's Warabi factory in Kawaguchi, Saitama on 17 April. The two-car set A was then unveiled to the press at Nippon Sharyo on 25 April 1962.
Test running was performed on the 32 km "model track" test section between Kamonomiya in Odawara and Ayase in Kanagawa prefecture from 26 June 1962. A speed of 190 km/h (118 mph) was first recorded on 27 October 1962 by set B, breaking the previous record of 175 km/h (109 mph) set by the narrow-gauge (1,067 mm (3 ft 6 in)) KuMoYa 93 test train on 21 November 1960. A speed of 200 km/h (124 mph) was first recorded on 31 October 1962 by set B, 210 km/h (130 mph) was reached on 20 December 1962, 243 km/h (151 mph) was recorded on 19 March 1963, and on 30 March 1963, set B recorded a world speed record of 256 km/h (159 mph).
With the start of test-running  and trial service on the Tōkaidō Shinkansen between Tokyo and Osaka in June 1964, set A was reclassified as a Class 941 emergency relief train (cars 941-1 and 941-2), and set B as a Class 922 track and overhead wire inspection train (set T1) (cars 922-1–4). The Class 941 remained out of use at Osaka Depot, and the Class 922 set was active until the new Class 922/10 set, T2, was delivered in 1974.
All six prototype cars were cut up at Hamamatsu Works between 1975 and 1976, being used to test the cutting-up facilities ahead of the first batch of 360 0 series cars due for withdrawal.
Class 1000 Shinkansen