The nave measures 55 feet (17 m) by 19 feet (5.8 m).
The north and south arcades have four bays; the north has round piers with moulded bases and capitals and the south has octagonal columns, also with moulded bases and capitals.
There is an inner and an outer aisle to the north, separated from each other by an arcade of four pointed arches and octagonal piers.
The font is octagonal with concave sides, each featuring a shield.
The shields contain symbols of the Passion and the initials of Lawrence Towneley.
The pulpit is constructed of oak on a stone base.

The chancel measures 42 feet 9 inches (13.03 m) by 20 feet 9 inches (6.32 m).
It has a narrow opening into the north aisle.
Its arcades to the north and south have octagonal piers and three pointed arches of two chamfered orders.
The Banastre chapel to the north, and the Barnside chapel to the south, are both separated from the aisles by oak screens.

The tower has a ring of eight bells.
Six of them date from 1814, cast by Thomas Mears of London.
The remaining two were given to the church in 1900 by Thomas Hyde of Colne.
The nave measures 55 feet (17 m) by 19 feet (5.8 m). The north and south arcades have four bays; the north has round piers with moulded bases and capitals and the south has octagonal columns, also with moulded bases and capitals. There is an inner and an outer aisle to the north, separated from each other by an arcade of four pointed arches and octagonal piers. The font is octagonal with concave sides, each featuring a shield. The shields contain symbols of the Passion and the initials of Lawrence Towneley. The pulpit is constructed of oak on a stone base.
The chancel measures 42 feet 9 inches (13.03 m) by 20 feet 9 inches (6.32 m). It has a narrow opening into the north aisle. Its arcades to the north and south have octagonal piers and three pointed arches of two chamfered orders. The Banastre chapel to the north, and the Barnside chapel to the south, are both separated from the aisles by oak screens.
The tower has a ring of eight bells. Six of them date from 1814, cast by Thomas Mears of London. The remaining two were given to the church in 1900 by Thomas Hyde of Colne.
St Bartholomew's Church, Colne