I-165, US 60, and US 431 serve Owensboro, with US 431 terminating at the former US 60 Bypass (now signed US 60).
US 231 and US 60 form a partial beltway around Owensboro.
KY 81, KY 56, KY 331, KY 298, KY 54, and KY 144 also serve the city.

Owensboro-Daviess County Regional Airport serves, along with Evansville Regional Airport, as one of the region's primary commercial airports.

The Owensboro Transit System (OTS) offers bus transit to residents, and the Green River Intra-County Transit System (GRITS) offers specialized bus services to residents with disabilities who are not able to ride fixed-route public transportation buses.
I-165, US 60, and US 431 serve Owensboro, with US 431 terminating at the former US 60 Bypass (now signed US 60). US 231 and US 60 form a partial beltway around Owensboro. KY 81, KY 56, KY 331, KY 298, KY 54, and KY 144 also serve the city.
Owensboro-Daviess County Regional Airport serves, along with Evansville Regional Airport, as one of the region's primary commercial airports.
The Owensboro Transit System (OTS) offers bus transit to residents, and the Green River Intra-County Transit System (GRITS) offers specialized bus services to residents with disabilities who are not able to ride fixed-route public transportation buses.
Owensboro, Kentucky