Comparative 16S rRNA gene sequence analysis showed that this strain belongs to the family Coxiellaceae, order Legionellales of Gamma-proteobacteria, and the closest relatives are different Rickettsiella spp.
A 1476-bp fragment of the rrs gene encoding the 16S ribosomal RNA was amplified and sequenced.
Surprisingly, a BLAST search did not result in a close similarity with any published 16S RNA gene sequences from other bacteria.
The level of 16S rRNA gene sequence similarity between strain 20B and other recognized species of the family was below 94.5%.
Partial sequences of the rpoB, parC and ftsY genes confirmed the phylogenetic position of the new isolate.
The G+C content estimated on the basis of whole genome analysis of strain 20B was 37.88%.
On the basis of its phenotypic and genotypic properties, together with phylogenetic distinctiveness, Mediannikov et al.
proposed that strain 20B to be classified in the new genus Diplorickettsia as the type strain of a novel species named Diplorickettsia massiliensis sp.
nov.
Comparative 16S rRNA gene sequence analysis showed that this strain belongs to the family Coxiellaceae, order Legionellales of Gamma-proteobacteria, and the closest relatives are different Rickettsiella spp. A 1476-bp fragment of the rrs gene encoding the 16S ribosomal RNA was amplified and sequenced. Surprisingly, a BLAST search did not result in a close similarity with any published 16S RNA gene sequences from other bacteria. The level of 16S rRNA gene sequence similarity between strain 20B and other recognized species of the family was below 94.5%. Partial sequences of the rpoB, parC and ftsY genes confirmed the phylogenetic position of the new isolate. The G+C content estimated on the basis of whole genome analysis of strain 20B was 37.88%. On the basis of its phenotypic and genotypic properties, together with phylogenetic distinctiveness, Mediannikov et al. proposed that strain 20B to be classified in the new genus Diplorickettsia as the type strain of a novel species named Diplorickettsia massiliensis sp. nov.
Diplorickettsia massiliensis