Nembrotha lineolata has been observed feeding on tunicates such as Oxycorynia fascicularis, Clavelina spp.
and  Rhopalaea spp.. It can insert its extensible oral tube through the tunicate's siphon and suck out the soft interior.

It lays its eggs in a yellowish-brown spiral ribbon and it is likely that the larvae that hatch out of these are free-swimming veliger larvae and form part of the plankton.
Known predators of nudibranchs include fish, crabs, sea spiders and polychaete worms.
There have been two instances when a sea anemone in the Edwardsiidae family has been observed attempting to feed on nudibranchs.
In one attack on Nembrotha lineolata, the sea slug flinched whenever it was touched by a tentacle but oozed a great deal of mucus and managed to struggle out of reach.
It is unclear whether sea anemones normally prey on sea slugs or whether these are unusual occurrences.
Nembrotha lineolata has been observed feeding on tunicates such as Oxycorynia fascicularis, Clavelina spp. and  Rhopalaea spp.. It can insert its extensible oral tube through the tunicate's siphon and suck out the soft interior.
It lays its eggs in a yellowish-brown spiral ribbon and it is likely that the larvae that hatch out of these are free-swimming veliger larvae and form part of the plankton. Known predators of nudibranchs include fish, crabs, sea spiders and polychaete worms. There have been two instances when a sea anemone in the Edwardsiidae family has been observed attempting to feed on nudibranchs. In one attack on Nembrotha lineolata, the sea slug flinched whenever it was touched by a tentacle but oozed a great deal of mucus and managed to struggle out of reach. It is unclear whether sea anemones normally prey on sea slugs or whether these are unusual occurrences.
Nembrotha lineolata