Arnolfo was born in Colle Val d'Elsa, Tuscany.

He was Nicola Pisano’s chief assistant on the marble Siena Cathedral Pulpit for the Duomo in Siena Cathedral (1265–1268), but he soon began to work independently on an important tomb sculpture.
In 1266–1267 he worked in Rome for King Charles I of Anjou, portraying him in the famous statue housed in the Campidoglio.
Around 1282 he finished the monument to Cardinal Guillaume de Braye in the church of San Domenico in Orvieto, including an enthroned Madonna (a Maestà) for which he took as a model an ancient Roman statue of the goddess Abundantia; the Madonna's tiara and jewels reproduce antique models.
In Rome Arnolfo had seen the Cosmatesque art, and its influence can be seen in the intarsia and polychrome glass decorations in the Basilica of Saint Paul Outside the Walls and the church Santa Cecilia in Trastevere, where he worked in 1285 and 1293 respectively.
In this period he also worked on the presepio of Santa Maria Maggiore, on Santa Maria in Aracoeli, on the monument of Pope Boniface VIII (1300) and on the bronze statue of St. Peter in St. Peter's Basilica.

In 1294–1295 he worked in Florence, mainly as an architect.
According to his biographer Giorgio Vasari, he was in charge of construction of the cathedral of the city, for which he provided the statues once decorating the lower part of the façade destroyed in 1589.
The surviving statues are now in the Museum of the Cathedral.
While the design of the Church of Santa Croce has been attributed to Arnolfo, this is highly disputed.
Vasari also attributed to him the urban plan of the new city of San Giovanni Valdarno.

The monumental character of Arnolfo's work has left its mark on the appearance of Florence.
His funerary monuments became the model for Gothic funerary art.

Giorgio Vasari included a biography of Arnolfo in his Lives of the Most Excellent Painters, Sculptors, and Architects.
Arnolfo was born in Colle Val d'Elsa, Tuscany.
He was Nicola Pisano’s chief assistant on the marble Siena Cathedral Pulpit for the Duomo in Siena Cathedral (1265–1268), but he soon began to work independently on an important tomb sculpture. In 1266–1267 he worked in Rome for King Charles I of Anjou, portraying him in the famous statue housed in the Campidoglio. Around 1282 he finished the monument to Cardinal Guillaume de Braye in the church of San Domenico in Orvieto, including an enthroned Madonna (a Maestà) for which he took as a model an ancient Roman statue of the goddess Abundantia; the Madonna's tiara and jewels reproduce antique models. In Rome Arnolfo had seen the Cosmatesque art, and its influence can be seen in the intarsia and polychrome glass decorations in the Basilica of Saint Paul Outside the Walls and the church Santa Cecilia in Trastevere, where he worked in 1285 and 1293 respectively. In this period he also worked on the presepio of Santa Maria Maggiore, on Santa Maria in Aracoeli, on the monument of Pope Boniface VIII (1300) and on the bronze statue of St. Peter in St. Peter's Basilica. 
In 1294–1295 he worked in Florence, mainly as an architect. According to his biographer Giorgio Vasari, he was in charge of construction of the cathedral of the city, for which he provided the statues once decorating the lower part of the façade destroyed in 1589. The surviving statues are now in the Museum of the Cathedral. While the design of the Church of Santa Croce has been attributed to Arnolfo, this is highly disputed. Vasari also attributed to him the urban plan of the new city of San Giovanni Valdarno.
The monumental character of Arnolfo's work has left its mark on the appearance of Florence. His funerary monuments became the model for Gothic funerary art. 
Giorgio Vasari included a biography of Arnolfo in his Lives of the Most Excellent Painters, Sculptors, and Architects.
Arnolfo di Cambio