By October 5, the Turks had finished their siege works and assembled all their artillery, which included nine basilisks, five great bombards, fifteen heavy guns, and 80 medium and smaller cannon that bombarded the fortress for the following 27 days.
That night, 5 more craft from Goa with gunpowder and reinforcements arrived.
After seven days of bombardment, part of the bulwark of Gaspar de Sousa collapsed and the Turks attempted to scale it "with two banners", but were repelled with heavy losses to bombs and arquebus fire.
Another assault the following morning was met with equally fierce resistance by the Portuguese.
Afterwards, the Turks forced labourers into the moat to undermine the fortress' walls and, in spite of several losses, managed to open a breach with gunpowder, but already the Portuguese had raised a barricade around the breach from the inside, which caused many losses on the assailants once they attempted to break through.
When at night the bombardment ceased, the Portuguese repaired the fortress' walls under the cover of darkness.

From an artillery battery on the opposite shore, the Turks bombarded the "Sea Fort" (Baluarte do Mar) that stood in the middle of the river mouth bombarding the flank of Muslim positions.
On October 27, Suleiman Pasha ordered 6 small galleys to attempt to scale the fortlet, but came under heavy Portuguese cannon fire.
The following day, the Turks drew 12 galleys and again attempted to "board" the fortlet, but were repelled with heavy losses due to fire bombs.

On October 30, Pasha Suleiman attempted a final diversion by faking the withdrawal of his forces, embarking 1,000 men.
Ever cautious, António da Silveira ordered the sentries to be alert – at daybreak, 14,000 men divided in three "banners" attempted to scale the fortress as it was bombarded with no regard to friendly fire.
A few hundred troops managed to scale the walls and raise banners but the Portuguese managed to repel the assailants, killing 500 and wounding a further 1,000 from gunfire and bombs out of the São Tomé bastion.

With his relation with Coja Safar and the Gujaratis degrading and increasingly fearful of being caught off-hand by the Viceroy's armada, on November 1 the Pasha finally decided to abandon the siege and began re-embarking his troops.
Suspecting another ruse from the Pasha, Captain Silveira ordered 20 of his last men on a sortie to deceive the enemy of their dwindling forces.
The party managed to capture a Turkish banner.

The Pasha however, intended on departing on November 5, but was unable due to unfavourable weather.
That night, two small galleys reached Diu with reinforcements and supplies, firing their guns and signal rockets.
The following morning, a fleet of 24 small galleys was sighted and believing it to be the vanguard of the governor's rescue fleet, the Pasha hurriedly departed, leaving 1,200 dead and 500 wounded behind.
Khadjar Safar then set fire to his encampment and abandoned the island with his forces shortly after.
In reality, it was just a forward fleet under the command of António da Silva Meneses and Dom Luís de Ataíde, dispatched from Goa with reinforcements, supplies, and news that the governor would depart soon to their aid.
Although they took no part in the fighting, the small force was triumphantly received within the ruined fortress by its last survivors.
The Portuguese were by then critically low on gunpowder and supplies and with less than 40 valid men; in the final stages of the siege, the Portuguese record that even the women assisted in its defence.
Catarina Lopes and Isabel Madeira are examples of two female captains who actively participated during the siege, they led a squad of female soldiers.
By October 5, the Turks had finished their siege works and assembled all their artillery, which included nine basilisks, five great bombards, fifteen heavy guns, and 80 medium and smaller cannon that bombarded the fortress for the following 27 days. That night, 5 more craft from Goa with gunpowder and reinforcements arrived. After seven days of bombardment, part of the bulwark of Gaspar de Sousa collapsed and the Turks attempted to scale it "with two banners", but were repelled with heavy losses to bombs and arquebus fire. Another assault the following morning was met with equally fierce resistance by the Portuguese. Afterwards, the Turks forced labourers into the moat to undermine the fortress' walls and, in spite of several losses, managed to open a breach with gunpowder, but already the Portuguese had raised a barricade around the breach from the inside, which caused many losses on the assailants once they attempted to break through. When at night the bombardment ceased, the Portuguese repaired the fortress' walls under the cover of darkness.
From an artillery battery on the opposite shore, the Turks bombarded the "Sea Fort" (Baluarte do Mar) that stood in the middle of the river mouth bombarding the flank of Muslim positions. On October 27, Suleiman Pasha ordered 6 small galleys to attempt to scale the fortlet, but came under heavy Portuguese cannon fire. The following day, the Turks drew 12 galleys and again attempted to "board" the fortlet, but were repelled with heavy losses due to fire bombs.
On October 30, Pasha Suleiman attempted a final diversion by faking the withdrawal of his forces, embarking 1,000 men. Ever cautious, António da Silveira ordered the sentries to be alert – at daybreak, 14,000 men divided in three "banners" attempted to scale the fortress as it was bombarded with no regard to friendly fire. A few hundred troops managed to scale the walls and raise banners but the Portuguese managed to repel the assailants, killing 500 and wounding a further 1,000 from gunfire and bombs out of the São Tomé bastion.
With his relation with Coja Safar and the Gujaratis degrading and increasingly fearful of being caught off-hand by the Viceroy's armada, on November 1 the Pasha finally decided to abandon the siege and began re-embarking his troops. Suspecting another ruse from the Pasha, Captain Silveira ordered 20 of his last men on a sortie to deceive the enemy of their dwindling forces. The party managed to capture a Turkish banner.
The Pasha however, intended on departing on November 5, but was unable due to unfavourable weather. That night, two small galleys reached Diu with reinforcements and supplies, firing their guns and signal rockets. The following morning, a fleet of 24 small galleys was sighted and believing it to be the vanguard of the governor's rescue fleet, the Pasha hurriedly departed, leaving 1,200 dead and 500 wounded behind. Khadjar Safar then set fire to his encampment and abandoned the island with his forces shortly after. In reality, it was just a forward fleet under the command of António da Silva Meneses and Dom Luís de Ataíde, dispatched from Goa with reinforcements, supplies, and news that the governor would depart soon to their aid. Although they took no part in the fighting, the small force was triumphantly received within the ruined fortress by its last survivors. The Portuguese were by then critically low on gunpowder and supplies and with less than 40 valid men; in the final stages of the siege, the Portuguese record that even the women assisted in its defence. Catarina Lopes and Isabel Madeira are examples of two female captains who actively participated during the siege, they led a squad of female soldiers.
Siege of Diu