Ann Street Presbyterian Church was listed on the Queensland Heritage Register on 21 October 1992 having satisfied the following criteria.

The place is important in demonstrating the evolution or pattern of Queensland's history.

The Ann Street Presbyterian Church is significant as one of the oldest churches still in use in Queensland, it is important in demonstrating the pattern of development of churches in Queensland's history.

The place is important in demonstrating the principal characteristics of a particular class of cultural places.

The building demonstrates the principal characteristics of the Presbyterian tradition as a church building, with a harmonious sequence of later additions.

The place is important because of its aesthetic significance.

The church is important in exhibiting particular aesthetic characteristics valued by the community including the quality and intactness of the interior of the church and the contribution the church makes to the townscape and to King George Square.

The place has a strong or special association with a particular community or cultural group for social, cultural or spiritual reasons.

As the site of continuous Presbyterian worship since 1858, the Ann Street Presbyterian Church has a strong and special association with the Presbyterian community for social, cultural and spiritual reasons.

The place has a special association with the life or work of a particular person, group or organisation of importance in Queensland's history.

The church has a special association with architect Alexander Brown Wilson who was associated with the Presbyterian church.
Ann Street Presbyterian Church was listed on the Queensland Heritage Register on 21 October 1992 having satisfied the following criteria.
The place is important in demonstrating the evolution or pattern of Queensland's history.
The Ann Street Presbyterian Church is significant as one of the oldest churches still in use in Queensland, it is important in demonstrating the pattern of development of churches in Queensland's history.
The place is important in demonstrating the principal characteristics of a particular class of cultural places.
The building demonstrates the principal characteristics of the Presbyterian tradition as a church building, with a harmonious sequence of later additions.
The place is important because of its aesthetic significance.
The church is important in exhibiting particular aesthetic characteristics valued by the community including the quality and intactness of the interior of the church and the contribution the church makes to the townscape and to King George Square.
The place has a strong or special association with a particular community or cultural group for social, cultural or spiritual reasons.
As the site of continuous Presbyterian worship since 1858, the Ann Street Presbyterian Church has a strong and special association with the Presbyterian community for social, cultural and spiritual reasons.
The place has a special association with the life or work of a particular person, group or organisation of importance in Queensland's history.
The church has a special association with architect Alexander Brown Wilson who was associated with the Presbyterian church.
Ann Street Presbyterian Church