In the initial period of operations, the C301 operated on Tamsui-Xindian and Beitou-Nanshijiao routes via Chiang Kai-shek Memorial Hall and Guting.
Following the opening of the new Dongmen station in 2012, the Beitou-Nanshijiao route was amended to become Beitou-Taipower Building route; this route was again modified with the opening of the Xinyi Line and instead terminated at Xiangshan.

With the opening of the Songshan Line in 2014, the Tamsui-Xindian route was withdrawn and the C301 trains are now confined to the Tamsui-Xinyi Line.
Trainsets 027/028 and 037/038 performed the final departures on the Tamsui-Xindian route on 14 November 2014.

Train set 013/014 had been previously split and modified to serve as 3-car formations on the Xinbeitou Branch Line.
When new 3-car formation C371 cars were introduced, train set 013/014 was subsequently re-joined and put back into normal service on Tamsui–Xinyi line from 22 July 2006 onward.
In the initial period of operations, the C301 operated on Tamsui-Xindian and Beitou-Nanshijiao routes via Chiang Kai-shek Memorial Hall and Guting. Following the opening of the new Dongmen station in 2012, the Beitou-Nanshijiao route was amended to become Beitou-Taipower Building route; this route was again modified with the opening of the Xinyi Line and instead terminated at Xiangshan.
With the opening of the Songshan Line in 2014, the Tamsui-Xindian route was withdrawn and the C301 trains are now confined to the Tamsui-Xinyi Line. Trainsets 027/028 and 037/038 performed the final departures on the Tamsui-Xindian route on 14 November 2014.
Train set 013/014 had been previously split and modified to serve as 3-car formations on the Xinbeitou Branch Line. When new 3-car formation C371 cars were introduced, train set 013/014 was subsequently re-joined and put back into normal service on Tamsui–Xinyi line from 22 July 2006 onward.
Taipei Metro C301