On Titan launches, the Titan booster would launch the IUS, carrying the payload into low Earth orbit where it was separated from the Titan and ignited its first stage, which carried it into an elliptical "transfer" orbit to a higher altitude.

On Shuttle launches, the orbiter's payload bay was opened, the IUS and its payload raised (by the IUS Airborne Support Equipment (ASE)) to a 50-52° angle, and released.
After the Shuttle separated from the payload to a safe distance, the IUS first stage ignited and, as on a Titan booster mission, entered a "transfer orbit".

Upon reaching apogee in the transfer orbit, the first stage and interstage structure were jettisoned.
 The second stage then fired to circularize the orbit, after which it released the satellite and, using its attitude control jets, began a retrograde maneuver to enter a lower orbit to avoid any possibility of collision with its payload.

In addition to the Communication and Reconnaissance missions described above, which placed the payload into stationary (24-hour) orbit, the IUS was also used to boost spacecraft towards planetary trajectories.
For these missions, the second IUS stage was separated and ignited immediately after first stage burnout.
Igniting the second stage at low altitude (and thus, high orbital speed) provided the extra velocity the spacecraft needed to escape from Earth orbit (see Oberth effect).
IUS could not impart as much velocity to its payload as Centaur would have been able to: while Centaur could have launched Galileo directly on a two-year trip to Jupiter, the IUS required a six-year voyage with multiple gravity assists.

The final flight of the IUS occurred in February 2004.
On Titan launches, the Titan booster would launch the IUS, carrying the payload into low Earth orbit where it was separated from the Titan and ignited its first stage, which carried it into an elliptical "transfer" orbit to a higher altitude. 
On Shuttle launches, the orbiter's payload bay was opened, the IUS and its payload raised (by the IUS Airborne Support Equipment (ASE)) to a 50-52° angle, and released. After the Shuttle separated from the payload to a safe distance, the IUS first stage ignited and, as on a Titan booster mission, entered a "transfer orbit". 
Upon reaching apogee in the transfer orbit, the first stage and interstage structure were jettisoned.  The second stage then fired to circularize the orbit, after which it released the satellite and, using its attitude control jets, began a retrograde maneuver to enter a lower orbit to avoid any possibility of collision with its payload.
In addition to the Communication and Reconnaissance missions described above, which placed the payload into stationary (24-hour) orbit, the IUS was also used to boost spacecraft towards planetary trajectories. For these missions, the second IUS stage was separated and ignited immediately after first stage burnout. Igniting the second stage at low altitude (and thus, high orbital speed) provided the extra velocity the spacecraft needed to escape from Earth orbit (see Oberth effect). IUS could not impart as much velocity to its payload as Centaur would have been able to: while Centaur could have launched Galileo directly on a two-year trip to Jupiter, the IUS required a six-year voyage with multiple gravity assists.
The final flight of the IUS occurred in February 2004.
Inertial Upper Stage