From late 1970, the UH-1N re-equipped the USAF 20th Special Operations Squadron in Vietnam, replacing the single-engined UH-1F and UH-1P. Armed with miniguns (or 40 mm grenade launchers) and rocket pods, and painted camouflage with no US markings and only a Green Hornet insignia, the UH-1N supported Special Forces reconnaissance missions from Cam Ranh Bay.

The United States Air Force employs UH-1Ns to fulfill its ICBM mission, providing a utility helicopter for transport between bases such as Minot AFB, Francis E. Warren AFB and Malmstrom AFB to missile launch sites in North Dakota, Montana, Wyoming, Nebraska, and Colorado.
 The UH-1N is also used by the 36th Rescue Squadron (36 RQS) at Fairchild AFB, Washington, for conducting search-and-rescue and medical evacuation missions, as well as the 459th Airlift Squadron based at Yokota Air Base in Japan.

During the 1982 Falklands War, the Argentine Air Force deployed two Bell 212s to Goose Green grass airstrip from where they performed general support duties including the recovery of many downed pilots.
By the end of the hostilities, both aircraft were still intact, but were dismantled by the Argentinians.

UH-1Ns were used by the USMC during its 2003 invasion of Iraq.
 UH-1Ns provided reconnaissance and communications support to Marine ground troops.
 They were also called upon to provide close air support during heavy fighting in Nasiriyah.

In August 2013, the Air Force said they were close to finalizing a plan to sustain and modernize their UH-1Ns for the next six to 10 years.
 The plan was to sustain the fleet, address flight and safety mandates, investigate modest improvements in capabilities, and reduce capability gaps.
 While the Huey is one of the oldest platforms in the service, keeping the fleet going was seen as having "minimal risk".
 Fleet-wide upgrades included night vision-compatible cockpit lighting, crash-worthy seats for flight engineers, and installation of a helicopter terrain-awareness warning system and traffic collision-avoidance device.
 The Air Force was also in the process of further acquiring ex-Marine Corps UH-1N models.
 The Marines were working out a strategy to transfer as many as 26 helicopters to the Air Force to either add them to their active fleet or keep them in reserve.

The UH-1N has seen combat service in the Colombian armed conflict.
On 16 October 2013, a UH-1N helicopter crashed in the northern La Guajira department.
The zone of the crash is a hotbed of FARC rebel activity.

The USMC planned to retire the UH-1N Iroquois by September 2014 after 43 years of service.
 Marine Light Attack Helicopter Squadron 773 was the last Marine squadron operating them, with their last deployment occurring in 2013, when two helicopters sailed on a Royal Netherlands Navy ship for an African Partnership Station deployment.
 Deliveries began to the Navy and Marine Corps in 1971, totaling 205 UH-1Ns and six VH-1N executive transports for Marine Helicopter Squadron One.
 The UH-1N was replaced by the upgraded UH-1Y Venom; 10 are remanufactured N-model airframes, after which the Marines decided to procure newly built Y-model airframes.
 Five unarmed HH-1N versions remained in use by the Marines until they were also replaced by UH-1Ys and retired in 2015, the only HH-1Ns remaining from 44 that were converted from 38 UH-1Ns and the six VH-1Ns.
The last combat deployment of the UH-1N by the Marines was to Afghanistan in 2010.
 The Marines retired the UH-1N during a "sundown ceremony" at Naval Air Station Joint Reserve Base New Orleans on 28 August 2014.

In March 2013, the U.S. Air Force operated 62 UH-1N Hueys with 25 providing security at ICBM sites, 19 stationed at Joint Base Andrews to evacuate Washington-based government officials in emergencies, and 18 used for testing and training.
Since most were purchased in 1969, the Air Force had sought a replacement.
The first requirements were issued in 2007, but the process was repeatedly delayed.

On 24 September 2018, the Air Force declared a Boeing/Leonardo submission of their MH-139 version of the AgustaWestland AW
From late 1970, the UH-1N re-equipped the USAF 20th Special Operations Squadron in Vietnam, replacing the single-engined UH-1F and UH-1P. Armed with miniguns (or 40 mm grenade launchers) and rocket pods, and painted camouflage with no US markings and only a Green Hornet insignia, the UH-1N supported Special Forces reconnaissance missions from Cam Ranh Bay.
The United States Air Force employs UH-1Ns to fulfill its ICBM mission, providing a utility helicopter for transport between bases such as Minot AFB, Francis E. Warren AFB and Malmstrom AFB to missile launch sites in North Dakota, Montana, Wyoming, Nebraska, and Colorado.  The UH-1N is also used by the 36th Rescue Squadron (36 RQS) at Fairchild AFB, Washington, for conducting search-and-rescue and medical evacuation missions, as well as the 459th Airlift Squadron based at Yokota Air Base in Japan.
During the 1982 Falklands War, the Argentine Air Force deployed two Bell 212s to Goose Green grass airstrip from where they performed general support duties including the recovery of many downed pilots. By the end of the hostilities, both aircraft were still intact, but were dismantled by the Argentinians.
UH-1Ns were used by the USMC during its 2003 invasion of Iraq.  UH-1Ns provided reconnaissance and communications support to Marine ground troops.  They were also called upon to provide close air support during heavy fighting in Nasiriyah.
In August 2013, the Air Force said they were close to finalizing a plan to sustain and modernize their UH-1Ns for the next six to 10 years.  The plan was to sustain the fleet, address flight and safety mandates, investigate modest improvements in capabilities, and reduce capability gaps.  While the Huey is one of the oldest platforms in the service, keeping the fleet going was seen as having "minimal risk".  Fleet-wide upgrades included night vision-compatible cockpit lighting, crash-worthy seats for flight engineers, and installation of a helicopter terrain-awareness warning system and traffic collision-avoidance device.  The Air Force was also in the process of further acquiring ex-Marine Corps UH-1N models.  The Marines were working out a strategy to transfer as many as 26 helicopters to the Air Force to either add them to their active fleet or keep them in reserve.
The UH-1N has seen combat service in the Colombian armed conflict. On 16 October 2013, a UH-1N helicopter crashed in the northern La Guajira department. The zone of the crash is a hotbed of FARC rebel activity.
The USMC planned to retire the UH-1N Iroquois by September 2014 after 43 years of service.  Marine Light Attack Helicopter Squadron 773 was the last Marine squadron operating them, with their last deployment occurring in 2013, when two helicopters sailed on a Royal Netherlands Navy ship for an African Partnership Station deployment.  Deliveries began to the Navy and Marine Corps in 1971, totaling 205 UH-1Ns and six VH-1N executive transports for Marine Helicopter Squadron One.  The UH-1N was replaced by the upgraded UH-1Y Venom; 10 are remanufactured N-model airframes, after which the Marines decided to procure newly built Y-model airframes.  Five unarmed HH-1N versions remained in use by the Marines until they were also replaced by UH-1Ys and retired in 2015, the only HH-1Ns remaining from 44 that were converted from 38 UH-1Ns and the six VH-1Ns. The last combat deployment of the UH-1N by the Marines was to Afghanistan in 2010.  The Marines retired the UH-1N during a "sundown ceremony" at Naval Air Station Joint Reserve Base New Orleans on 28 August 2014.
In March 2013, the U.S. Air Force operated 62 UH-1N Hueys with 25 providing security at ICBM sites, 19 stationed at Joint Base Andrews to evacuate Washington-based government officials in emergencies, and 18 used for testing and training. Since most were purchased in 1969, the Air Force had sought a replacement. The first requirements were issued in 2007, but the process was repeatedly delayed.
On 24 September 2018, the Air Force declared a Boeing/Leonardo submission of their MH-139 version of the AgustaWestland AW
Bell UH-1N Twin Huey