Neptune formed part of the weather column in the Battle of Trafalgar on 21 October, and was the third ship from the lead, situated between her sister HMS Temeraire, and the 74-gun HMS Leviathan.
Fremantle had been promised a position second to Nelson aboard HMS Victory, and by 10 o'clock was sailing fast enough to threaten to overtake her.
Fremantle hoped to pass her, and lead the line into battle, but Nelson ordered 'Neptune, take in your studding-sails and drop astern.
I shall break the line myself.'
Neptune went into action with her band playing, and everyone except the officers and the band lying down on the deck to protect them from enemy fire.
Ahead of her Fremantle saw Eliab Harvey's Temeraire turn to pass astern of the French Redoutable, but resolved to follow Nelson and HMS Victory to pass astern of the French flagship Bucentaure.
As she passed under Bucentaure's stern, Neptune discharged a double-shotted broadside from her larboard (port) guns, with devastating consequences on Villeneuve's already disabled flagship.
Fremantle then had the helm swung hard to starboard, bringing his ship abeam of the Bucentaure.
He fired two more triple-shotted broadsides from nearly 50 guns at a range of less than 100 yards into the beleaguered French ship.

Fremantle then spotted the towering mass of the Spanish four-decker Santísima Trinidad sailing away from him, and steered towards her starboard quarter in the hope of raking her stern.
Opening fire with his larboard battery, he positioned Neptune off the Spanish vessel's starboard beam and the two exchanged heavy fire for the next hour as more British ships poured through the gap astern of Neptune.
Neptune took fire from other ships of the combined fleet as they sailed past.
Santísima Trinidad, heavily battered by Neptune's guns, as well as those from the 74-gun ships HMS Leviathan and HMS Conqueror, became completely dismasted and covered in debris.
She fought on until 5.30 pm, when she struck her colours, having sustained casualties of 205 dead and 103 wounded.
Neptune left the 98-gun HMS Prince to take possession and headed north to cut off the remains of the enemy fleet, briefly becoming engaged with the French 74-gun Intrépide.
During the battle Neptune suffered considerable damage to her masts, although they did not fall.
Most of her rigging was cut to pieces and she sustained nine shot holes in her hull.
She sustained casualties of ten killed and 34 wounded.
A remarkably small proportion of her officers became casualties, with only the captain's clerk, Richard Hurrell, being wounded.

After the battle Collingwood transferred his flag from the damaged HMS Royal Sovereign to the frigate HMS Euryalus, and on 22 October Neptune took the Royal Sovereign in tow.
On 23 October, as the Franco-Spanish forces that had escaped into Cadiz sortied under Commodore Julien Cosmao, Neptune cast off the tow, surrendering the duty to HMS Mars, and took on board Villeneuve and several captured flag captains, who had originally been aboard Mars.
As the weather continued to deteriorate Neptune sent her boats to assist in the evacuation of the Santísima Trinidad before she foundered.
After riding out the storm she took the battered Victory, carrying Lord Nelson's body, in tow on 26 October and brought her into Gibraltar on 28 October.
Neptune formed part of the weather column in the Battle of Trafalgar on 21 October, and was the third ship from the lead, situated between her sister HMS Temeraire, and the 74-gun HMS Leviathan. Fremantle had been promised a position second to Nelson aboard HMS Victory, and by 10 o'clock was sailing fast enough to threaten to overtake her. Fremantle hoped to pass her, and lead the line into battle, but Nelson ordered 'Neptune, take in your studding-sails and drop astern. I shall break the line myself.' Neptune went into action with her band playing, and everyone except the officers and the band lying down on the deck to protect them from enemy fire. Ahead of her Fremantle saw Eliab Harvey's Temeraire turn to pass astern of the French Redoutable, but resolved to follow Nelson and HMS Victory to pass astern of the French flagship Bucentaure. As she passed under Bucentaure's stern, Neptune discharged a double-shotted broadside from her larboard (port) guns, with devastating consequences on Villeneuve's already disabled flagship. Fremantle then had the helm swung hard to starboard, bringing his ship abeam of the Bucentaure. He fired two more triple-shotted broadsides from nearly 50 guns at a range of less than 100 yards into the beleaguered French ship.
Fremantle then spotted the towering mass of the Spanish four-decker Santísima Trinidad sailing away from him, and steered towards her starboard quarter in the hope of raking her stern. Opening fire with his larboard battery, he positioned Neptune off the Spanish vessel's starboard beam and the two exchanged heavy fire for the next hour as more British ships poured through the gap astern of Neptune. Neptune took fire from other ships of the combined fleet as they sailed past. Santísima Trinidad, heavily battered by Neptune's guns, as well as those from the 74-gun ships HMS Leviathan and HMS Conqueror, became completely dismasted and covered in debris. She fought on until 5.30 pm, when she struck her colours, having sustained casualties of 205 dead and 103 wounded. Neptune left the 98-gun HMS Prince to take possession and headed north to cut off the remains of the enemy fleet, briefly becoming engaged with the French 74-gun Intrépide. During the battle Neptune suffered considerable damage to her masts, although they did not fall. Most of her rigging was cut to pieces and she sustained nine shot holes in her hull. She sustained casualties of ten killed and 34 wounded. A remarkably small proportion of her officers became casualties, with only the captain's clerk, Richard Hurrell, being wounded.
After the battle Collingwood transferred his flag from the damaged HMS Royal Sovereign to the frigate HMS Euryalus, and on 22 October Neptune took the Royal Sovereign in tow. On 23 October, as the Franco-Spanish forces that had escaped into Cadiz sortied under Commodore Julien Cosmao, Neptune cast off the tow, surrendering the duty to HMS Mars, and took on board Villeneuve and several captured flag captains, who had originally been aboard Mars. As the weather continued to deteriorate Neptune sent her boats to assist in the evacuation of the Santísima Trinidad before she foundered. After riding out the storm she took the battered Victory, carrying Lord Nelson's body, in tow on 26 October and brought her into Gibraltar on 28 October.
HMS Neptune (1797)