On 7 April, Greek authorities announced that they were charging the captain and five other officers with negligence.
State television reported they were charged with causing a shipwreck through negligence, breaching international shipping safety regulations and polluting the environment.
Additional charges could be made depending on the fate of the two missing passengers.
All six were released until further notice but if convicted they could face a five-year prison sentence.

On 13 April 2007, it was reported that investigators using a remotely controlled submarine found the ship's data recorder (VDR).
In a struggle to protect the data, they secured it in a special sterilized bin and the authorities were to have transferred it to the United States in order to reveal the saved data, an action that only the manufacturer of the VDR can perform.
The Greek Merchant Marine Ministry said the recorder could reveal details of the sinking of the ship which could be used in the prosecution of crew members.

DNV, one of the world's leading classification agencies, stated in their press release that "At the time of the accident, DNV had issued class certificate, safety management certificate and ship security certificate.
Sea Diamond had no overdue surveys and no condition of class".
For the Sea Diamond to have been issued a Class certificate, her water-tight doors would have to have been fully operational.

Investigations carried out by the defense team of the Master of the Vessel and Louis Cruise Lines, after a lawsuit had been filed against them, have included a new hydrographic survey of the area of the accident in Santorini.
This survey was carried out by Akti Engineering, and discovered discrepancies between the actual mapping of the sea area and the official charts used by the Sea Diamond (and all other vessels) at the time of the accident.
The detailed survey claimed that the reef, which the Sea Diamond struck is, in fact, lying at 131 metres (430 ft) from shore and not at a distance of 57 metres (187 ft), as is incorrectly marked on the nautical chart.
The official chart also shows the depth of the water at the area of impact varying from 18–22 metres (59–72 ft), whilst the recent survey shows that it is only 5 metres (16 ft).

The findings obtained by Akti Engineering have since been passed on to the Hellenic Hydrographic Office of the Hellenic Navy and other responsible authorities, with the aim that the necessary changes to maritime charts should be made and similar accidents to be prevented.
According to a branch reviewing source, the Hellenic Hydrographic Office initially rejected the new mapping, but a later study confirmed the findings of Akti.

A Greek scuba diver gathering evidence for a judicial investigation of the Sea Diamond shipwreck died in October 2007 from decompression sickness after ascending too quickly.
On 7 April, Greek authorities announced that they were charging the captain and five other officers with negligence. State television reported they were charged with causing a shipwreck through negligence, breaching international shipping safety regulations and polluting the environment. Additional charges could be made depending on the fate of the two missing passengers. All six were released until further notice but if convicted they could face a five-year prison sentence.
On 13 April 2007, it was reported that investigators using a remotely controlled submarine found the ship's data recorder (VDR). In a struggle to protect the data, they secured it in a special sterilized bin and the authorities were to have transferred it to the United States in order to reveal the saved data, an action that only the manufacturer of the VDR can perform. The Greek Merchant Marine Ministry said the recorder could reveal details of the sinking of the ship which could be used in the prosecution of crew members.
DNV, one of the world's leading classification agencies, stated in their press release that "At the time of the accident, DNV had issued class certificate, safety management certificate and ship security certificate. Sea Diamond had no overdue surveys and no condition of class". For the Sea Diamond to have been issued a Class certificate, her water-tight doors would have to have been fully operational.
Investigations carried out by the defense team of the Master of the Vessel and Louis Cruise Lines, after a lawsuit had been filed against them, have included a new hydrographic survey of the area of the accident in Santorini. This survey was carried out by Akti Engineering, and discovered discrepancies between the actual mapping of the sea area and the official charts used by the Sea Diamond (and all other vessels) at the time of the accident. The detailed survey claimed that the reef, which the Sea Diamond struck is, in fact, lying at 131 metres (430 ft) from shore and not at a distance of 57 metres (187 ft), as is incorrectly marked on the nautical chart. The official chart also shows the depth of the water at the area of impact varying from 18–22 metres (59–72 ft), whilst the recent survey shows that it is only 5 metres (16 ft).
The findings obtained by Akti Engineering have since been passed on to the Hellenic Hydrographic Office of the Hellenic Navy and other responsible authorities, with the aim that the necessary changes to maritime charts should be made and similar accidents to be prevented. According to a branch reviewing source, the Hellenic Hydrographic Office initially rejected the new mapping, but a later study confirmed the findings of Akti.
A Greek scuba diver gathering evidence for a judicial investigation of the Sea Diamond shipwreck died in October 2007 from decompression sickness after ascending too quickly.
MS Sea Diamond