On 24 June 1618 she married Count Jacob De la Gardie.
After the marriage, she moved with him to Swedish Estonia, where he served as governor, and lived with him there from 1619 until 1628.
The relationship between Ebba Brahe and De la Gardie is described as happy, and the couple had fourteen children.
Because of the frequent absence of her spouse in service she was given the responsibility of the family finances, and she moved extensively between Sweden and Estonia to manage the family estates.
In 1628, the couple settled in Sweden.
Ebba Brahe became known for her extravagant way of living and her taste for luxury.
   
Brahe also played a part at the royal court.
In 1651, the historian Arnold Johan Messenius and his son accused her of having persuaded queen Christina not to marry by the use of witchcraft.
Such an accusation could not be accepted about a noble, and the accusation eventually lead to the accusers being decapitated for treason.
 
She favored her son Magnus Gabriel, and was said to have spoiled him and attending to his career.
She was proud over his position as favourite to Queen Christina of Sweden, and supported his marriage to the Queen's cousin Princess Maria Eufrosyne von Zweibrücken-Kleeburg.
When Magnus Gabriel lost his position as favorite with the queen in 1653, Ebba Brahe fell upon her knees before the queen to ask him to forgive him, and she also sought the help of Axel Oxenstierna to reinstate him in favor, though she privately blamed the Oxenstierna party for having caused the fall of Magnus Gabriel.
During the minor regency of Charles XI in 1660-1672, her son Magnus Gabriel was one of the leading members and rulers of Sweden.
Ebba Brahe actively used her influence over her son to put forward both her own and various supplicants demands, recommendations and suggestions.
 
Ebba Brahe also became known as a business person.
As a widow, Ebba Brahe became one of the many powerful female land holders, who occupy a significant place in the local legends of Sweden during the 16th and 17th centuries, such as Barbro Påle, Sophia Drake, Margareta Huitfeldt and Margareta von Ascheberg.
She became a successful land holder, managed her estates and expanded them.
She acquired new estates from the formerly Danish province Skåne, which became a Swedish province in 1658 and had many large estates which were left behind by the Danish nobility.

After her husband's death in 1652 she successfully petitioned Queen Christina I of Sweden to found the city of Jakobstad in Finland, named in honor of her late husband.
A street in Jakobstad, Ebba Brahe Esplanaden, has been named after her.
On 24 June 1618 she married Count Jacob De la Gardie. After the marriage, she moved with him to Swedish Estonia, where he served as governor, and lived with him there from 1619 until 1628. The relationship between Ebba Brahe and De la Gardie is described as happy, and the couple had fourteen children. Because of the frequent absence of her spouse in service she was given the responsibility of the family finances, and she moved extensively between Sweden and Estonia to manage the family estates. In 1628, the couple settled in Sweden. Ebba Brahe became known for her extravagant way of living and her taste for luxury.    
Brahe also played a part at the royal court. In 1651, the historian Arnold Johan Messenius and his son accused her of having persuaded queen Christina not to marry by the use of witchcraft. Such an accusation could not be accepted about a noble, and the accusation eventually lead to the accusers being decapitated for treason.  
She favored her son Magnus Gabriel, and was said to have spoiled him and attending to his career. She was proud over his position as favourite to Queen Christina of Sweden, and supported his marriage to the Queen's cousin Princess Maria Eufrosyne von Zweibrücken-Kleeburg. When Magnus Gabriel lost his position as favorite with the queen in 1653, Ebba Brahe fell upon her knees before the queen to ask him to forgive him, and she also sought the help of Axel Oxenstierna to reinstate him in favor, though she privately blamed the Oxenstierna party for having caused the fall of Magnus Gabriel. During the minor regency of Charles XI in 1660-1672, her son Magnus Gabriel was one of the leading members and rulers of Sweden. Ebba Brahe actively used her influence over her son to put forward both her own and various supplicants demands, recommendations and suggestions.  
Ebba Brahe also became known as a business person. As a widow, Ebba Brahe became one of the many powerful female land holders, who occupy a significant place in the local legends of Sweden during the 16th and 17th centuries, such as Barbro Påle, Sophia Drake, Margareta Huitfeldt and Margareta von Ascheberg. She became a successful land holder, managed her estates and expanded them. She acquired new estates from the formerly Danish province Skåne, which became a Swedish province in 1658 and had many large estates which were left behind by the Danish nobility. 
After her husband's death in 1652 she successfully petitioned Queen Christina I of Sweden to found the city of Jakobstad in Finland, named in honor of her late husband. A street in Jakobstad, Ebba Brahe Esplanaden, has been named after her.
Ebba Brahe