Pearce began playing with Darebin in the Victorian Women's Football League (VWFL) in 2005 at the age of 16.
She was a member of the Australian team that played against Ireland in the 2006 Ladies' International Rules Series, which remains the only women's series to have been played.
In June 2007, at the age of 19, Pearce was one of two VWFL representatives, alongside St Albans Spurs midfielder Shannon McFerran, who played in the annual E. J. Whitten Legends Game; Pearce played for Victoria while McFerran played for the All Stars, who won by 15 points.

Pearce captained the Victoria under-19 team at the 2007 AFL Women's National Championships.
She was named in the Victoria under-19 All-Australian team and applauded for her efforts during the week-long carnival, winning the award for joint-player of the tournament.
Pearce led the team to a premiership with a 47-point win against South Australia.

In May 2013, Pearce was selected by Melbourne with the first selection in the inaugural national women's draft to play in the first women's exhibition game; among the other Melbourne draftees were future Melbourne AFLW teammates Melissa Hickey and Bianca Jakobsson.
Pearce was one of fifty women to make history in June when Melbourne's women's team took on the Western Bulldogs' women's team at the Melbourne Cricket Ground in the first of what would be several exhibition games between the two clubs, played as a curtain raiser to the men's match between the same two clubs.
Pearce captained the team in each of the games from 2013 to 2016, and was the inaugural winner of the club's best female player award at the end of the 2014 season, polling five out of a possible six votes.
Pearce began playing with Darebin in the Victorian Women's Football League (VWFL) in 2005 at the age of 16. She was a member of the Australian team that played against Ireland in the 2006 Ladies' International Rules Series, which remains the only women's series to have been played. In June 2007, at the age of 19, Pearce was one of two VWFL representatives, alongside St Albans Spurs midfielder Shannon McFerran, who played in the annual E. J. Whitten Legends Game; Pearce played for Victoria while McFerran played for the All Stars, who won by 15 points.
Pearce captained the Victoria under-19 team at the 2007 AFL Women's National Championships. She was named in the Victoria under-19 All-Australian team and applauded for her efforts during the week-long carnival, winning the award for joint-player of the tournament. Pearce led the team to a premiership with a 47-point win against South Australia.
In May 2013, Pearce was selected by Melbourne with the first selection in the inaugural national women's draft to play in the first women's exhibition game; among the other Melbourne draftees were future Melbourne AFLW teammates Melissa Hickey and Bianca Jakobsson. Pearce was one of fifty women to make history in June when Melbourne's women's team took on the Western Bulldogs' women's team at the Melbourne Cricket Ground in the first of what would be several exhibition games between the two clubs, played as a curtain raiser to the men's match between the same two clubs. Pearce captained the team in each of the games from 2013 to 2016, and was the inaugural winner of the club's best female player award at the end of the 2014 season, polling five out of a possible six votes.
Daisy Pearce