Engineering work on the East London line extension started in 2005 and the underground service ended in December 2007.

In 2007 London Buses route ELW Whitechapel – Shadwell – Wapping was introduced, operating every 10 minutes, every 15 minutes at evenings and weekends.
It was operated with route-branded single-deck buses.
Starting on 23 December 2007 it was extended from Whitechapel to Shoreditch (Monday-Friday 07:00-10:30 & 15:30–20:30, Sunday 07:00-15:30) from 19 July 2008.
The frequency of the route was cut to four buses per hour in September 2009.
It was reduced to weekends-only from 28 April 2010, and withdrawn on 9 May 2010.

Between 2006 and May 2008 other rail replacement buses were provided.
Route ELS Whitechapel – Shoreditch (Monday-Friday 07:00-10:30 & 15:30–20:30, Sunday 07:00-15:30) commenced 10 June 2006 and was withdrawn on 19 July 2008.
It was replaced by a peak-hour extension of route ELW.

London Buses route ELC New Cross Gate – New Cross – Surrey Quays – Canada Water (Monday-Friday every 5–10 minutes, weekends every 15 minutes) started on 23 December 2007.
It was withdrawn on 25 September 2009 following a 40% drop in passenger numbers.
Transport for London estimated that this saved around £1 million over the period to June 2010.

London Buses route ELP Canada Water – Rotherhithe (every 15 minutes) began on 23 December 2007 and was withdrawn on 24 February 2008 due to lack of use: tickets were valid between Bermondsey and Canada Water on standard route 381.

Unlike the previous East London line closure, no replacement bus service was provided across the River Thames through the Rotherhithe Tunnel.
London Transport was concerned that demand would be so high and buses would be so small that the frequency could have to be one bus every 30 seconds.
There was also an issue that the Rotherhithe Tunnel is restricted to vehicles with a width no more than 7 feet (2.1 m), which had been employed on previous occasions.
However, by 2007 no such vehicles were commercially available that were low-floor compliant: the only ones with this width, as previously used, were minibuses with higher floors.
As bus policy by this time was 100% low floor, it meant that no such service could be provided.

A limited train service was introduced on 27 April 2010 and full service began on 23 May 2010.
Engineering work on the East London line extension started in 2005 and the underground service ended in December 2007.
In 2007 London Buses route ELW Whitechapel – Shadwell – Wapping was introduced, operating every 10 minutes, every 15 minutes at evenings and weekends. It was operated with route-branded single-deck buses. Starting on 23 December 2007 it was extended from Whitechapel to Shoreditch (Monday-Friday 07:00-10:30 & 15:30–20:30, Sunday 07:00-15:30) from 19 July 2008. The frequency of the route was cut to four buses per hour in September 2009. It was reduced to weekends-only from 28 April 2010, and withdrawn on 9 May 2010.
Between 2006 and May 2008 other rail replacement buses were provided. Route ELS Whitechapel – Shoreditch (Monday-Friday 07:00-10:30 & 15:30–20:30, Sunday 07:00-15:30) commenced 10 June 2006 and was withdrawn on 19 July 2008. It was replaced by a peak-hour extension of route ELW.
London Buses route ELC New Cross Gate – New Cross – Surrey Quays – Canada Water (Monday-Friday every 5–10 minutes, weekends every 15 minutes) started on 23 December 2007. It was withdrawn on 25 September 2009 following a 40% drop in passenger numbers. Transport for London estimated that this saved around £1 million over the period to June 2010.
London Buses route ELP Canada Water – Rotherhithe (every 15 minutes) began on 23 December 2007 and was withdrawn on 24 February 2008 due to lack of use: tickets were valid between Bermondsey and Canada Water on standard route 381.
Unlike the previous East London line closure, no replacement bus service was provided across the River Thames through the Rotherhithe Tunnel. London Transport was concerned that demand would be so high and buses would be so small that the frequency could have to be one bus every 30 seconds. There was also an issue that the Rotherhithe Tunnel is restricted to vehicles with a width no more than 7 feet (2.1 m), which had been employed on previous occasions. However, by 2007 no such vehicles were commercially available that were low-floor compliant: the only ones with this width, as previously used, were minibuses with higher floors. As bus policy by this time was 100% low floor, it meant that no such service could be provided.
A limited train service was introduced on 27 April 2010 and full service began on 23 May 2010.
East London line