The Milton Caniff Collection consists of 12,000 original artworks by Caniff, 85 boxes of memorabilia, and more than 450 boxes of manuscript materials, fan letters, and business records.

As the museum's collection of original art and manuscripts evolved and expanded, it added the Nick Anderson Collection, the Jim Borgman Collection, the Eldon Dedini Collection, the Edwina Dumm Collection, the Woody Gelman Collection of Winsor McCay cartoons, the Walt Kelly Collection, the collection of agent Toni Mendez, and the Bill Watterson Collection.
The Bud Blake Collection includes more than 5,800 of the cartoon panels he drew for King Features Syndicate from 1954 to 1965, plus 10,000 daily and Sunday Tiger originals.
Comic-book collections include the Will Eisner Collection; the Jay Kennedy Collection has more than 9,500 underground comic books.

The museum's collection includes work by Anne Mergen, who was the only female editorial cartoonist in the United States for much of her career.

Archival professional records include the Association of American Editorial Cartoonists, National Cartoonists Society, Newspaper Features Council, and the Cartoonists Guild.
A biographical registry of cartoonists contains files for more than 5,000 cartoonists and clipping files organized by cartoon-related subjects.
The Milton Caniff Collection consists of 12,000 original artworks by Caniff, 85 boxes of memorabilia, and more than 450 boxes of manuscript materials, fan letters, and business records.
As the museum's collection of original art and manuscripts evolved and expanded, it added the Nick Anderson Collection, the Jim Borgman Collection, the Eldon Dedini Collection, the Edwina Dumm Collection, the Woody Gelman Collection of Winsor McCay cartoons, the Walt Kelly Collection, the collection of agent Toni Mendez, and the Bill Watterson Collection. The Bud Blake Collection includes more than 5,800 of the cartoon panels he drew for King Features Syndicate from 1954 to 1965, plus 10,000 daily and Sunday Tiger originals. Comic-book collections include the Will Eisner Collection; the Jay Kennedy Collection has more than 9,500 underground comic books.
The museum's collection includes work by Anne Mergen, who was the only female editorial cartoonist in the United States for much of her career.
Archival professional records include the Association of American Editorial Cartoonists, National Cartoonists Society, Newspaper Features Council, and the Cartoonists Guild. A biographical registry of cartoonists contains files for more than 5,000 cartoonists and clipping files organized by cartoon-related subjects.
Billy Ireland Cartoon Library & Museum