Cornus florida is easily propagated by seeds, which are sown in the fall into prepared rows of sawdust or sand, and emerge in the spring.
Germination rates for good clean seed should be near 100% if seed dormancy is first overcome by cold stratification treatments for 90 to 120 days at 4 °C (39 °F).

Flowering dogwood demonstrates gametophytic self-incompatibility, meaning that the plants can't self-fertilize.
This is important for breeding programs as it means that it is not necessary to emasculate (remove the anthers from) C. florida flowers before making controlled cross-pollinations.
These pollinations should be repeated every other day, as the flowers must be cross-pollinated within one or two days of opening for pollinations to be effective.

Softwood cuttings taken in late spring or early summer from new growth can be rooted under mist if treated with 8,000 to 10,000 ppm indole-3-butyric acid (IBA).
In cold climates, potted cuttings must be kept in heated cold frames or polyhouses the following winter to maintain temperatures between 0 and 7 °C (32 and 45 °F).
Although rooting success can be as high as 50–85%, this technique is not commonly used by commercial growers.
Rather, selected cultivars are generally propagated by T-budding in late summer or by whip grafting in the greenhouse in winter onto seedling rootstock.

Micropropagation of flowering dogwood is now used in breeding programs aiming to incorporate resistance to dogwood anthracnose and powdery mildew into horticulturally and economically important cultivars.
Nodal (axillary bud) sections are established in a culture of Woody Plant Medium (WPM) amended with 4.4 µmol/L 6-Benzyladenine (BA) to promote shoot growth.
 Rooting of up to 83% can be obtained when 5–7 week-old microshoots are then transferred to WPM amended with 4.9 µmol/L IBA.
Cornus florida is easily propagated by seeds, which are sown in the fall into prepared rows of sawdust or sand, and emerge in the spring. Germination rates for good clean seed should be near 100% if seed dormancy is first overcome by cold stratification treatments for 90 to 120 days at 4 °C (39 °F). 
Flowering dogwood demonstrates gametophytic self-incompatibility, meaning that the plants can't self-fertilize. This is important for breeding programs as it means that it is not necessary to emasculate (remove the anthers from) C. florida flowers before making controlled cross-pollinations. These pollinations should be repeated every other day, as the flowers must be cross-pollinated within one or two days of opening for pollinations to be effective.
Softwood cuttings taken in late spring or early summer from new growth can be rooted under mist if treated with 8,000 to 10,000 ppm indole-3-butyric acid (IBA). In cold climates, potted cuttings must be kept in heated cold frames or polyhouses the following winter to maintain temperatures between 0 and 7 °C (32 and 45 °F). Although rooting success can be as high as 50–85%, this technique is not commonly used by commercial growers. Rather, selected cultivars are generally propagated by T-budding in late summer or by whip grafting in the greenhouse in winter onto seedling rootstock.
Micropropagation of flowering dogwood is now used in breeding programs aiming to incorporate resistance to dogwood anthracnose and powdery mildew into horticulturally and economically important cultivars. Nodal (axillary bud) sections are established in a culture of Woody Plant Medium (WPM) amended with 4.4 µmol/L 6-Benzyladenine (BA) to promote shoot growth.  Rooting of up to 83% can be obtained when 5–7 week-old microshoots are then transferred to WPM amended with 4.9 µmol/L IBA.
Cornus florida