EF65 1: Preserved at the Kyoto Railway Museum in Kyoto.

EF65 5: JR Freight Ōi Depot in Shinagawa, Tokyo (training use)
EF65 520: Usui Pass Railway Heritage Park, Gunma Prefecture
EF65 535: Originally stored at Ōmiya Works, Saitama Prefecture, and donated to Toshiba in Fuchu, Tokyo in March 2013
EF65 536: Sekisui Kinzoku factory, Saitama Prefecture (cab only)
EF65 539: Privately preserved in Gunma Prefecture (cab only)
EF65 1001: JR Freight Ōi Depot in Shinagawa, Tokyo (training use)
EF65 1: Preserved at the Kyoto Railway Museum in Kyoto.
EF65 5: JR Freight Ōi Depot in Shinagawa, Tokyo (training use)
EF65 520: Usui Pass Railway Heritage Park, Gunma Prefecture
EF65 535: Originally stored at Ōmiya Works, Saitama Prefecture, and donated to Toshiba in Fuchu, Tokyo in March 2013
EF65 536: Sekisui Kinzoku factory, Saitama Prefecture (cab only)
EF65 539: Privately preserved in Gunma Prefecture (cab only)
EF65 1001: JR Freight Ōi Depot in Shinagawa, Tokyo (training use)
JNR Class EF65