The squadron arrived in England equipped with late model B-17F aircraft equipped with "Tokyo Tanks", additional fuel cells located outboard in the wings that gave this model additional range.
It flew its first combat mission on 13 May 1943 against an airfield near Saint-Omer, France.
 For the next two months the squadron focused on attacking airfields and V-1 flying bomb launch sites in France.

Eighth Air Force's early experience with its Martin B-26 Marauders convinced it that the Marauders were stationed too far from the continent of Europe to reach a selection of targets.
It determined to move them closer to the target areas, and an exchange of bases began.
 The entire 95th group moved to RAF Horham in June, where they replaced the 323d Bombardment Group, which departed the previous day.
A few days later their place at Framlingham was taken by the newly arrived 390th Bombardment Group.

The 335th began strategic bombing operations in July and continued until flying its last operation on 20 April 1945.
 Its targets included harbors, marshalling yards and other industrial targets along with attacks on cities.
 The squadron received its first Distinguished Unit Citation (DUC) during an attack on an aircraft factory at Regensburg, Germany on 17 August 1943 when it maintained its defensive formation despite severe attacks by enemy interceptor aircraft.

On 10 October, during an attack on marshalling yards at Münster, Germany, the squadron was subjected to concentrated fighter attacks on the approach to the target and intense flak over the objective.
Despite these obstacles, the formation's bombs were clustered close to the target.
It was awarded a second DUC for withstanding these attacks to bomb its objective.
 From 20 to 25 February 1944 the squadron participated in the Big Week offensive against the German aircraft manufacturing industry.
 A few days later, on 4 March, the squadron attacked Berlin despite adverse weather that led other units to either abandon the operation or attack secondary targets.
 Despite snowstorms and heavy cloud cover, the unit struck its target while under attack from enemy fighters, although the cloud cover required the group to rely on a pathfinder from the 482d Bombardment Group to determine the release point.
It received its third DUC for this operation.
This mission was the first time any unit from Eighth Air Force had bombed Berlin.

The squadron was diverted to bombing priority tactical targets during the preparation for and execution of Operation Overlord, the invasion of Normandy in June 1944, attacking communications and coastal defenses.
 It hit enemy troop concentrations to facilitate the Allied breakout at Saint-Lô.
 The 335th attacked enemy troop concentrations during the Battle of the Bulge from December 1944 to January 1945 and bombed airfields to support Operation Varsity, the airborne assault across the Rhine in March.

One of the unit's more unusual missions was flown on 18 September 1944, when the 95th group led the 13th Combat Bombardment Wing to Warsaw to drop ammunition, food and medical supplies to Polish resistance forces fighting against German occupation forces, landing at bases in the Soviet Union.
 The squadron had previously participated in shuttle missions to the Soviet Union.

The unit flew its last mission on 20 April 1945, when it attacked marshalling yards near Oranienburg.
 In the first week of May, it airdropped food to Dutch citizens in Operation Chow Hound.
From V-E Day until departing the theater in June, it transported liberated prisoners of war and displaced persons.
The air echelon flew their planes back to Bradley Field, Connecticut, while the ground echelon sailed once more on the Queen Elizabeth.
The squadron was reunited at Sioux Falls Army Air Field, South Dakota, where it was inactivated on 28 August 1945.
The squadron arrived in England equipped with late model B-17F aircraft equipped with "Tokyo Tanks", additional fuel cells located outboard in the wings that gave this model additional range. It flew its first combat mission on 13 May 1943 against an airfield near Saint-Omer, France.  For the next two months the squadron focused on attacking airfields and V-1 flying bomb launch sites in France.
Eighth Air Force's early experience with its Martin B-26 Marauders convinced it that the Marauders were stationed too far from the continent of Europe to reach a selection of targets. It determined to move them closer to the target areas, and an exchange of bases began.  The entire 95th group moved to RAF Horham in June, where they replaced the 323d Bombardment Group, which departed the previous day. A few days later their place at Framlingham was taken by the newly arrived 390th Bombardment Group.
The 335th began strategic bombing operations in July and continued until flying its last operation on 20 April 1945.  Its targets included harbors, marshalling yards and other industrial targets along with attacks on cities.  The squadron received its first Distinguished Unit Citation (DUC) during an attack on an aircraft factory at Regensburg, Germany on 17 August 1943 when it maintained its defensive formation despite severe attacks by enemy interceptor aircraft.
On 10 October, during an attack on marshalling yards at Münster, Germany, the squadron was subjected to concentrated fighter attacks on the approach to the target and intense flak over the objective. Despite these obstacles, the formation's bombs were clustered close to the target. It was awarded a second DUC for withstanding these attacks to bomb its objective.  From 20 to 25 February 1944 the squadron participated in the Big Week offensive against the German aircraft manufacturing industry.  A few days later, on 4 March, the squadron attacked Berlin despite adverse weather that led other units to either abandon the operation or attack secondary targets.  Despite snowstorms and heavy cloud cover, the unit struck its target while under attack from enemy fighters, although the cloud cover required the group to rely on a pathfinder from the 482d Bombardment Group to determine the release point. It received its third DUC for this operation. This mission was the first time any unit from Eighth Air Force had bombed Berlin.
The squadron was diverted to bombing priority tactical targets during the preparation for and execution of Operation Overlord, the invasion of Normandy in June 1944, attacking communications and coastal defenses.  It hit enemy troop concentrations to facilitate the Allied breakout at Saint-Lô.  The 335th attacked enemy troop concentrations during the Battle of the Bulge from December 1944 to January 1945 and bombed airfields to support Operation Varsity, the airborne assault across the Rhine in March.
One of the unit's more unusual missions was flown on 18 September 1944, when the 95th group led the 13th Combat Bombardment Wing to Warsaw to drop ammunition, food and medical supplies to Polish resistance forces fighting against German occupation forces, landing at bases in the Soviet Union.  The squadron had previously participated in shuttle missions to the Soviet Union.
The unit flew its last mission on 20 April 1945, when it attacked marshalling yards near Oranienburg.  In the first week of May, it airdropped food to Dutch citizens in Operation Chow Hound. From V-E Day until departing the theater in June, it transported liberated prisoners of war and displaced persons. The air echelon flew their planes back to Bradley Field, Connecticut, while the ground echelon sailed once more on the Queen Elizabeth. The squadron was reunited at Sioux Falls Army Air Field, South Dakota, where it was inactivated on 28 August 1945.
335th Bombardment Squadron