Guelph was the first municipality in Canada to have its own federally chartered railway, the Guelph Junction Railway.
This 25 kilometre (16 miles) link to the CPR is still municipally owned.

Built in 1911, the Guelph Central Station (still in use), was constructed by the Grand Trunk Railway which had arrived in Guelph in 1856; years later, it was taken over by the Canadian National Railway.
 It is a classic example of early 20th Century Canadian railway station design and has been designated as a heritage structure under the Heritage Railway Stations Protection Act.
The Romanesque Revival building, with its Italianate tower, has been listed on the Canadian Register since 2006 and was formally recognized as one of Canada's Historic Places in November 1992.
A renovation project in 2016-2017 provided various benefits, including repairs to maintain and restore heritage aspects.

There had also been passenger stations in Guelph that were built by the Canadian Pacific Railway.
The first CPR station, from the 1800s, was the Priory House station, converted from the first house in Guelph.
It stood opposite the current Priory Square and was eventually dismantled.
Its replacement, located between Cardigan Street and the Speed River, was a brick building erected in 1911.
After this brick building was no longer used as a rail station, it was converted for other purposes; eventually it was moved to the Galt area of Cambridge, Ontario.

The Central station is currently an Intermodal Transit Terminal that includes bus and railway services in one facility.
The following is a summary of its purpose from an April 2017 report:
"Guelph Central Train Station is a busy transit hub that accommodates Guelph Transit, GO Transit, Via Rail and Greyhound Canada operations.
Each weekday, more than 5,000 passengers board Guelph Transit, to travel on one of the 15 different routes that operate out of the bus bays adjacent to the train station."
Guelph was the first municipality in Canada to have its own federally chartered railway, the Guelph Junction Railway. This 25 kilometre (16 miles) link to the CPR is still municipally owned.
Built in 1911, the Guelph Central Station (still in use), was constructed by the Grand Trunk Railway which had arrived in Guelph in 1856; years later, it was taken over by the Canadian National Railway.  It is a classic example of early 20th Century Canadian railway station design and has been designated as a heritage structure under the Heritage Railway Stations Protection Act. The Romanesque Revival building, with its Italianate tower, has been listed on the Canadian Register since 2006 and was formally recognized as one of Canada's Historic Places in November 1992. A renovation project in 2016-2017 provided various benefits, including repairs to maintain and restore heritage aspects.
There had also been passenger stations in Guelph that were built by the Canadian Pacific Railway. The first CPR station, from the 1800s, was the Priory House station, converted from the first house in Guelph. It stood opposite the current Priory Square and was eventually dismantled. Its replacement, located between Cardigan Street and the Speed River, was a brick building erected in 1911. After this brick building was no longer used as a rail station, it was converted for other purposes; eventually it was moved to the Galt area of Cambridge, Ontario.
The Central station is currently an Intermodal Transit Terminal that includes bus and railway services in one facility. The following is a summary of its purpose from an April 2017 report:
"Guelph Central Train Station is a busy transit hub that accommodates Guelph Transit, GO Transit, Via Rail and Greyhound Canada operations. Each weekday, more than 5,000 passengers board Guelph Transit, to travel on one of the 15 different routes that operate out of the bus bays adjacent to the train station."
Guelph