There are eight theme rooms in Museo Pambata, namely:
Kalikasan (Environment) and Karagatan (Under the Sea) - Contains a simulated rain forest and seabed where visitors can learn about environmental concerns and ecological conservation.

Maynila Noon (Old Manila) - Visitors can learn about history by looking at artifacts and simulated exhibits depicting turn-of-the-century Manila.

Paglaki Ko (Career Options) - Showcases various careers and encourages visitors to visualize themselves doing a particular career.
Currently it features writers and illustrators.

I Love My Planet Earth - Where visitors can learn about climate change and how one can take part in caring for the planet.

Pamilihang Bayan (Marketplace) - Features a row of play stores and shops.
One can pretend to be a store owner and practice their entrepreneurial skills.

Katawan Ko (My Body Works) - Features the human body, how various organs work, and pointers on healthcare.

Bata sa Mundo (Children in the Global Village) - Dolls from around the world are displayed in this room.

The museum also has a children's library, changing exhibits hall, gift shop, three venues for various events, playground, and parking space.
There are eight theme rooms in Museo Pambata, namely:
Kalikasan (Environment) and Karagatan (Under the Sea) - Contains a simulated rain forest and seabed where visitors can learn about environmental concerns and ecological conservation.
Maynila Noon (Old Manila) - Visitors can learn about history by looking at artifacts and simulated exhibits depicting turn-of-the-century Manila.
Paglaki Ko (Career Options) - Showcases various careers and encourages visitors to visualize themselves doing a particular career. Currently it features writers and illustrators.
I Love My Planet Earth - Where visitors can learn about climate change and how one can take part in caring for the planet.
Pamilihang Bayan (Marketplace) - Features a row of play stores and shops. One can pretend to be a store owner and practice their entrepreneurial skills.
Katawan Ko (My Body Works) - Features the human body, how various organs work, and pointers on healthcare.
Bata sa Mundo (Children in the Global Village) - Dolls from around the world are displayed in this room.
The museum also has a children's library, changing exhibits hall, gift shop, three venues for various events, playground, and parking space.
Museo Pambata