where is chromatin found and what 2 things is it made of
Chromatin
γH2AX , the phosphorylated form of H2AX is also involved in the early steps leading to chromatin decondensation after DNA damage occurrence . The histone variant H2AX constitutes about 10 % of the H2A histones in human chromatin . γH2AX ( H2AX phosphorylated on serine 139 ) can be detected as soon as 20 seconds after irradiation of cells ( with DNA double - strand break formation ) , and half maximum accumulation of γH2AX occurs in one minute . The extent of chromatin with phosphorylated γH2AX is about two million base pairs at the site of a DNA double - strand break . γH2AX does not , itself , cause chromatin decondensation , but within 30 seconds of irradiation , RNF8 protein can be detected in association with γH2AX . RNF8 mediates extensive chromatin decondensation , through its subsequent interaction with CHD4 , a component of the nucleosome remodeling and deacetylase complex NuRD .
γH2AX , the phosphorylated form of H2AX is also involved in the early steps leading to chromatin decondensation after DNA damage occurrence . The histone variant H2AX constitutes about 10 % of the H2A histones in human chromatin . γH2AX ( H2AX phosphorylated on serine 139 ) can be detected as soon as 20 seconds after irradiation of cells ( with DNA double - strand break formation ) , and half maximum accumulation of γH2AX occurs in one minute . The extent of chromatin with phosphorylated γH2AX is about two million base pairs at the site of a DNA double - strand break . γH2AX does not , itself , cause chromatin decondensation , but within 30 seconds of irradiation , RNF8 protein can be detected in association with γH2AX . RNF8 mediates extensive chromatin decondensation , through its subsequent interaction with CHD4 , a component of the nucleosome remodeling and deacetylase complex NuRD .