what is the purpose of a loading control in a western blot
Western blot normalization
Because stain - free technology does not require staining and destaining steps , it can be significantly faster than methods that utilize Ponceau S or Sypro Ruby stains . Consequently , it is becoming more popular , with an exponential growth in the number of publications using this technique . Gels developed with stain - free technology are compatible with standard SDS - PAGE buffers . Stain - free technology can also visualize proteins in the gel after electrophoresis or on the blot after transfer . Modifications to the proteins themselves are minimal and do not affect protein transfer or downstream antibody binding in western blotting . Furthermore , the observed intensity of the bands does not depend on the duration of staining / destaining and does not decrease with time .
Because stain - free technology does not require staining and destaining steps , it can be significantly faster than methods that utilize Ponceau S or Sypro Ruby stains . Consequently , it is becoming more popular , with an exponential growth in the number of publications using this technique . Gels developed with stain - free technology are compatible with standard SDS - PAGE buffers . Stain - free technology can also visualize proteins in the gel after electrophoresis or on the blot after transfer . Modifications to the proteins themselves are minimal and do not affect protein transfer or downstream antibody binding in western blotting . Furthermore , the observed intensity of the bands does not depend on the duration of staining / destaining and does not decrease with time .