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Migration rate dependency | Electrophoresis | Directly proportional to rate of movement | Charge of the molecule |
Migration rate dependency | Electrophoresis | Inversely proportional to rate of movement | Size of the molecule |
Migration rate dependency | Electrophoresis | Increased current increases migration rate | Electrical field |
Migration rate dependency | Electrophoresis | Increased ionic strength decreases migration rate | Ionic strength of buffer |
Migration rate dependency | Electrophoresis | Decreased pH slows migration | pH of buffer |
Migration rate dependency | Electrophoresis | Inversely proportional to migration rate | Viscosity of supporting medium |
Migration rate dependency | Electrophoresis | High temperature can denature protein and slow migration | System temperature |
Analytic electrophoretic procedures | Electrophoresis | Proteins are amphoteric... | Protein electrophoresis |
Analytic electrophoretic procedures | Electrophoresis | Similar to protein electrophoresis... | Isoenzyme electrophoresis |
Components | Immunoassay | A substance that can elicit an immune response... | Antigen |
Components | Immunoassay | An immunoglobulin formed in response to a foreign substance... | Antibody |
Immunochemical labels | Immunoassay | Attached to the antibody, allowing colorimetric quantitation... | Enzyme labels |
Immunochemical labels | Immunoassay | Emit photons when excited, used in fluorescence-based detection... | Fluorescent labels |
Immunochemical labels | Immunoassay | Release energy in the form of visible light upon returning to ground state... | Chemiluminescent labels |
Immunochemical labels | Immunoassay | Emit radiation (gamma rays) upon decay, detected for quantitation... | Radioisotope labels |
Methodologies | Immunoassay | Enzyme-based | ELISA |
Methodologies | Immunoassay | Enzyme-based | EIA/EMIT |
Methodologies | Immunoassay | Fluorescence-based | FPIA |
Methodologies | Immunoassay | Fluorescence-based | FIA |
Methodologies | Immunoassay | Radiation-based | RIA |
Methodologies | Immunoassay | Radiation-based | IRMA |
Mechanisms of separation | Chromatography | Based on the competition between the sample and the mobile phase for binding sites... | Adsorption chromatography |
Mechanisms of separation | Chromatography | Depends on the solubility of the solute in nonpolar or polar solvents... | Partition chromatography |
Mechanisms of separation | Chromatography | Involves separation of solutes by their size and charge of the ionic species present... | Ion-exchange chromatography |
Chromatographic procedures | Chromatography | Semi-quantitative screening test... | Thin-layer chromatography (TLC) |
Chromatographic procedures | Chromatography | Provides quantitative results... | High-performance liquid chromatography (HPLC) |
Migration rate dependency | Electrophoresis | Directly proportional to rate of movement | Charge of the molecule |
Migration rate dependency | Electrophoresis | Inversely proportional to rate of movement | Size of the molecule |
Migration rate dependency | Electrophoresis | Increased current increases migration rate | Electrical field |
Migration rate dependency | Electrophoresis | Increased ionic strength decreases migration rate | Ionic strength of buffer |
Migration rate dependency | Electrophoresis | Decreased pH slows migration | pH of buffer |
Migration rate dependency | Electrophoresis | Inversely proportional to migration rate | Viscosity of supporting medium |
Migration rate dependency | Electrophoresis | High temperature can denature protein and slow migration | System temperature |
Analytic electrophoretic procedures | Electrophoresis | Proteins are amphoteric... | Protein electrophoresis |
Analytic electrophoretic procedures | Electrophoresis | Similar to protein electrophoresis... | Isoenzyme electrophoresis |
Components | Immunoassay | A substance that can elicit an immune response... | Antigen |
Components | Immunoassay | An immunoglobulin formed in response to a foreign substance... | Antibody |
Immunochemical labels | Immunoassay | Attached to the antibody, allowing colorimetric quantitation... | Enzyme labels |
Immunochemical labels | Immunoassay | Emit photons when excited, used in fluorescence-based detection... | Fluorescent labels |
Immunochemical labels | Immunoassay | Release energy in the form of visible light upon returning to ground state... | Chemiluminescent labels |
Immunochemical labels | Immunoassay | Emit radiation (gamma rays) upon decay, detected for quantitation... | Radioisotope labels |
Methodologies | Immunoassay | Enzyme-based | ELISA |
Methodologies | Immunoassay | Enzyme-based | EIA/EMIT |
Methodologies | Immunoassay | Fluorescence-based | FPIA |
Methodologies | Immunoassay | Fluorescence-based | FIA |
Methodologies | Immunoassay | Radiation-based | RIA |
Methodologies | Immunoassay | Radiation-based | IRMA |
Mechanisms of separation | Chromatography | Based on the competition between the sample and the mobile phase for binding sites... | Adsorption chromatography |
Mechanisms of separation | Chromatography | Depends on the solubility of the solute in nonpolar or polar solvents... | Partition chromatography |
Mechanisms of separation | Chromatography | Involves separation of solutes by their size and charge of the ionic species present... | Ion-exchange chromatography |
Chromatographic procedures | Chromatography | Semi-quantitative screening test... | Thin-layer chromatography (TLC) |
Chromatographic procedures | Chromatography | Provides quantitative results... | High-performance liquid chromatography (HPLC) |