Below is an instruction from Human. Write a response.
    ### Instruction:
    How to diagnose Holt-Oram syndrome ?
    ### Response:
    How is Holt-Oram syndrome diagnosed? The diagnosis of Holt-Oram syndrome can be established based on physical features and family history. It can be confirmed through genetic testing looking for mutations in the TBX5 gene. Hand x-rays are usually performed for upper-limb malformations.
    