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The secretory immune system. | Several features distinguish mucosal from systemic immunity; depending on the anatomical and functional characteristics of the organs involved. Secretory IgA immunoglobulins are the main effectors of the mucosal immune system and their protective aspects are well documented. Serum IgA have received less attention but are equally important; since they control and remove in a non-phlogistic way antigens crossing the mucosal barriers. | 2634626 | [
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Cachectin/tumour necrosis factor-alpha in the circulation of patients with rheumatic disease. | By means of a sensitive double-antibody radioimmunoassay; cachectin/tumour necrosis factor-alpha (TNF) was measured in sera from 51 patients with rheumatic disease. Elevated levels of circulating cachectin/TNF were observed in 46% of patients with rheumatoid arthritis (RA; p less than 0.001 versus blood donors) and in 29% of patients with systemic lupus erythematosus (SLE; p less than 0.05 versus blood donors). Marked elevation of cachectin/TNF occurred in both RA and SLE patients in connection with severe | 2634628 | [
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Cachectin/tumour necrosis factor-alpha in the circulation of patients with rheumatic disease. | infections. The results show that cachectin/TNF is present in the circulation of certain patients with rheumatic disease; and that although the median cachectin TNF level in SLE patients is lower than that in RA patients; the cachectin/TNF response in SLE patients to severe infections is similar to that in RA patients. | 2634628 | [
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Anti-inflammatory activity of Teucrium polium. | Teucrium polium is widely used by the folk-medicine practitioners in Saudi Arabia for the treatment of inflammations; rheumatism; diabetes and ulcers. Scientific reports are lacking to confirm these activities. The present study reports the effect of ethanolic extract of T. polium on carrageenan-induced acute inflammation; cotton-pellet granuloma and some of the biochemical parameters. The ethanolic extract of Teucrium polium at a dose of 500 mg/kg body weight produced significant inhibition of carrageenan- | 2634627 | [
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Anti-inflammatory activity of Teucrium polium. | induced inflammation and cotton-pellet granuloma. Biochemical studies showed a significant decrease in glucose level. The presence of flavonoids and sterols might be responsible for the anti-inflammatory activity of this plant. Further studies on the fractionation of the phytoconstituents; and on their mechanism of action are in progress. | 2634627 | [
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Utilities and quality-adjusted life years. | Utilities and quality-adjusted life years (QALYs) are reviewed; with particular focus on their use in technology assessment. This article provides a broad overview and perspective on these two techniques and their interrelationship; with reference to other sources for details of implementation. The historical development; assumptions; strengths/weaknesses; and applications of each are summarized. Utilities are specifically designed for individual decision-making under uncertainty; but; with additional assum | 2634630 | [
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Utilities and quality-adjusted life years. | ptions; utilities can be aggregated across individuals to provide a group utility function. QALYs are designed to aggregate in a single summary measure the total health improvement for a group of individuals; capturing improvements from impacts on both quantity of life and quality of life--with quality of life broadly defined. Utilities can be used as the quality-adjustment weights for QALYs; they are particularly appropriate for that purpose; and this combination provides a powerful and highly useful varia | 2634630 | [
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Advances in prenatal diagnosis. Social-psychological and policy issues. | Chorionic villus sampling (CVS); a new technique for prenatally diagnosing chromosomal and genetic disorders; may soon replace amniocentesis. This procedure; performed by inserting a catheter through the pregnant woman's cervix or through the abdomen and removing cells from the placenta; has the advantages of being available earlier in the pregnancy than amniocentesis (at 9 to 11 weeks gestational age) and of yielding results in a shorter time. Although the medical aspects of the procedure are being investi | 2634631 | [
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Advances in prenatal diagnosis. Social-psychological and policy issues. | gated; its psychosocial and policy implications have not been studied systematically. These issues include the subjective assessment of risk that prompts women to choose or to reject CVS; the implications of earlier diagnosis for feelings about abortion; the potential of negative findings (i.e.; the absence of the designated disorder) for well-being during the remainder of the pregnancy; the ramifications of first trimester identification of fetal sex; equity of access to prenatal diagnosis; and the possibl | 2634631 | [
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Advances in prenatal diagnosis. Social-psychological and policy issues. | e over-utilization of these procedures. Drawing on previous research regarding amniocentesis and more recent research concerning CVS; this article discusses the potential implications of CVS. | 2634631 | [
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Outcome-based equity in the treatment of colon cancer patients in Finland. | The differences in survival rates of colon cancer patients between hospital districts were used as an indicator of equity in cancer treatment in Finland. The merits of two different assessment methods were evaluated. The large variation was mainly due to confounding by age and the extent of the disease and to random variation. | 2634632 | [
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Head injury. Variability of course and presence of confounding factors. | Review of a large data base on 76 patients who had suffered head injuries and whose metabolic changes were being studied demonstrated the variability of their neurological courses and the importance of considering confounding factors in the analysis of such a group. It was apparent that many such patients continue to change neurologically and functionally for long periods of time; and that their ultimate outcome cannot be determined for at least 1 year. The study demonstrates the inherent complexity of a gr | 2634633 | [
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Head injury. Variability of course and presence of confounding factors. | oup of patients with head injuries and the need to use sophisticated statistical tools and a computer to demonstrate whether apparent trends are statistically valid in light of confounding factors and variabilities. Failure to take this complexity into account seems to be a major factor in many of the ongoing controversies in research on head injuries. A possible solution to the problem of potential biases introduced by lost patients would be to develop a method to use government records to obtain current a | 2634633 | [
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Head injury. Variability of course and presence of confounding factors. | ddresses for patients involved in federally funded research. | 2634633 | [
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Multifocal fractures of the femur. | Multifocal fractures of the femur in modern traumatology are becoming more and more frequent and often pose difficult problems of treatment. In view of the sparse contributions on this subject in the literature; we analysed our series of 33 cases with a view to formulating our conclusions as to the best type of treatment. Because of the varied associations of different types of fracture in a single skeletal segment; there is no single method of synthesis applicable to all cases. The various means of synthes | 2634634 | [
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Multifocal fractures of the femur. | is at our disposal (Küntscher nail; Grosse Kempf blocked nail; Ender nail; AO plates and screws; external fixators) should be selected depending on the individual case; respecting the concept of closed surgery as much as possible. This requires imagination as well as experience. | 2634634 | [
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The results of reconstruction of the anterior cruciate ligament with semitendinosus and gracilis tendons in chronic laxity of the knee. | The authors report the medium term results (41 months) in 88 patients submitted to surgery for reconstruction of the anterior cruciate ligament according to the method currently in use at our Clinic which uses the semitendinosus and gracilis tendons. The results were evaluated according to a score card method and in 28 patients a KT-1000 arthrometer was also used. The method gives a probability of full success in approximately 85% of cases; with significantly better results in patients not submitted to meni | 2634635 | [
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The results of reconstruction of the anterior cruciate ligament with semitendinosus and gracilis tendons in chronic laxity of the knee. | scectomy. A comparison with other methods of autogenous transplant and prosthetic substitution; even on the basis of the data provided by the KT-1000; still places the semitendinosus and gracilis method at the forefront. | 2634635 | [
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A five year follow-up of 605 cases of the MCCL (metal-cancellous cementless Lübeck) total hip prosthesis. | The porous surface of the MCCL prosthesis macroscopically resembles spongy bone; with a porous depth of up to 10 mm; the width of the pores measuring 1-2 mm; with reticular spaces forming an intercommunicating system. The superficial porous surface and the implant stem are combined in a single structure. The standard prosthesis is made up of four components; an acetabulum in cobalt-chrome-molybdenum alloy; an interposed layer of polyethylene; a ceramic head and a stem in cobalt-chrome-molybdenum alloy; the | 2634636 | [
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A five year follow-up of 605 cases of the MCCL (metal-cancellous cementless Lübeck) total hip prosthesis. | shape of which is anatomically matched to the proximal femur. What emerges from the present study is the possibility of using right and left stems; each available in six sizes. The ceramic head may be attached to the neck of the femoral stem; which may be long; average; or short. An "extra-long" metal head is also available. | 2634636 | [
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The surgical treatment of supra-intercondylar fractures of the femur. | The authors report their results in the surgical treatment of supra-intercondylar fractures of the femur and compare the efficacy of the different methods used. Their analysis shows that anatomical restoration of the joint line and firm stabilisation of the supracondylar fragments in correct alignment with the metaphysis are the essential aims of surgery capable of producing satisfactory results. | 2634637 | [
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Arthroscopic synovectomy in the treatment of haemophilic arthropathy: preliminary results in eight patients. | Synovectomy is a procedure which is widely used in the surgical treatment of haemophilic arthropathy: the short and long-term results have in fact sufficiently shown its effectiveness in the reduction of the number of cases of haemarthrosis. This operation; however; has the disadvantage of a reduction in joint mobility which may vary from 25% to 77%; depending on the various case series reported (Post et al.; 1986; Clark; 1978; McCollough et al.; 1979; Montane et al.; 1986). This complication moreover; is c | 2634638 | [
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Arthroscopic synovectomy in the treatment of haemophilic arthropathy: preliminary results in eight patients. | ommon to all synovectomy operations; even those which are performed for synovial affections of a different nature. As the advantages of arthroscopic synovectomy as compared to open surgery are commonly known; it was decided to extend the indication to haemophilic arthropathy (Wiedel; 1985; Klein et al.; 1987). The purpose of this study is to evaluate the effectiveness of arthroscopic synovectomy in 8 patients with type A haemophilia affected with severe arthropathy consequent to repeated haemarthrosis and w | 2634638 | [
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Arthroscopic synovectomy in the treatment of haemophilic arthropathy: preliminary results in eight patients. | ith marked hypertrophic synovial tissue. | 2634638 | [
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Corrective femoral osteotomy and telescopic nailing in osteogenesis imperfecta. | Six cases of osteogenesis imperfecta with femoral deformity treated by Bailey telescopic nailing are discussed. In 4 cases the operation was bilateral. The long-term results show that this nail lengthens without compromising longitudinal growth of the bone; while providing protection from fracture and recurrence of the deformity. Thinning of the diaphyseal cortex was a significant complication in 3 femurs out of 10. Although it is more difficult to apply; the telescopic nail is preferable to traditional nai | 2634639 | [
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Corrective femoral osteotomy and telescopic nailing in osteogenesis imperfecta. | ling because of its better mechanical features which allow for early weightbearing and walking. | 2634639 | [
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Biological aspects of repair osteogenesis in corticospongy homologous grafts. | An experimental study was carried out aimed at studying the short-term; mid-term and long-term biological features of the incorporation of homologous bone grafts. Homologous cortical and corticospongy grafts of the proximal third of the tibia were implanted in 60 adult New Zealand rabbits; under general anaesthesia. The animals were sacrificed after predetermined periods of time; and the tibia removed for radiographic and histological examination. An analysis of the different phases of the fusion process al | 2634641 | [
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Biological aspects of repair osteogenesis in corticospongy homologous grafts. | lowed us to observe the different dynamic evolution of osteogenic repair of cortical as compared to spongy grafts. This could be explained by the different tensile properties of the two types of grafts. | 2634641 | [
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Bacteriological and histological study of 40 loose cemented hip prostheses. | The causes of the loosening of a hip prosthesis may be inflammatory; mechanical; immunitary or due to detritus. A bacteriological and histological study; in cases where further surgery was needed; may clearly contribute to the aetiopathogenesis of failure. Loose hip prostheses removed at operation were studied from a bacteriological and; in particular; histological point of view. There results were then correlated to clinical; radiographic and laboratory data obtained during follow-up. Inflammation proved t | 2634640 | [
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] |
Bacteriological and histological study of 40 loose cemented hip prostheses. | o be a constant histological finding. The hyalinosis observed in 22 cases revealed significant analogies with histological pictures of silicotic granuloma; for which an immunitary pathogenesis is the current hypothesis. Thus; it is our belief that this correlation is suggestive of an immunitary based approach in cases where hip prostheses fail due to loosening. | 2634640 | [
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Chronic osteomyelitis. A long-term review of 37 cases. | The authors carried out a long-term review of 37 patients suffering from chronic osteomyelitis with the aim of studying the natural history of the disease and identifying any prognostic factors during the initial stage of the disease. Analysis of the data failed to identify any reliable prognostic parameters available at the onset of the disease. However; the worst results were observed in cases of osteomyelitis which had occurred after open fractures. | 2634642 | [
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CT scan in a study of the normal anatomy of the hindfoot and midfoot. | The authors use CT scan to describe the anatomy of the hindfoot and midfoot. Initially the bones and related joints are examined; the latter being particularly complex and difficult to evaluate in this area with traditional radiology. Particular emphasis is placed on the description of the subtalar joint complex; to which all the bones of the hindfoot and midfoot contribute with the exception of the cuneiforms. This is followed by a description of the anatomical features of the soft tissues. The components | 2634643 | [
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CT scan in a study of the normal anatomy of the hindfoot and midfoot. | of the five areas into which the hindfoot and midfoot may be subdivided are illustrated; with particular emphasis on the structures of the medial region which contribute to the formation of the tarsal tunnel. Starting from a description of the anatomy of this area; the main neurovascular trunks; the fascial structures; ligaments and muscles in the various sites are defined. | 2634643 | [
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Mammography units. | We evaluated eight mammorgraphy units from five manufacturers; basing our tests on the units' screen-film imaging capability. Our ratings were based primarily on the units' ability to safely and consistently produce acceptable images with minimal patient dose. One unit is rated Acceptable-Preferred; two units are rated Acceptable; two units are rated Acceptable but not recommended for magnification applications; and three units are rated Conditionally Acceptable because of poor automatic exposure control pe | 2634645 | [
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The use of CT scan in evaluating Volkmann's syndrome in the limbs. | Between January 1980 and October 1986 26 cases of Volkmann's syndrome were submitted to preoperative CT scan. Thirteen were localised in the upper limb; 11 in the lower limb; and 2 were cases of pseudo-Volkmann's disease. CT scan allowed us to confirm the densitometric features and distribution of muscle infarction in the various muscle planes. Thus; two groups were distinguished; each with different densitometric features: a first group with diffuse hypodensity of the muscular sheaths with unclear borders; | 2634644 | [
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The use of CT scan in evaluating Volkmann's syndrome in the limbs. | and a coefficient of attenuation at approximately 30-40 H.U.; which at operation corresponded to diffuse fibrosis; a second group with defined hypodensity of the various muscle groups equal to 10-20 H.U.; which at operation gave a macroscopic picture of infarction. CT scan provides a detailed preoperative topographical and morphological evaluation of the infarction area; and therefore a treatment plan based on a more accurate knowledge of the anatomical and pathological lesions. | 2634644 | [
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Microprocessor-controlled third-generation critical care ventilators. | We evaluated five microprocessor-controlled critical care ventilators from five manufacturers. We rated three units Acceptable and two units Conditionally Acceptable; basing our ratings primarily on performance; safety; monitoring features; specific deficiencies; and ergonomic and human factors design. Because of differences in clinical practice and patient populations; we recommend that purchasing decisions be based on clinical trials conducted on the full range of patients for whom the ventilator will lik | 2634647 | [
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Patient transfer systems. | This evaluation of patient transfer systems was prepared to give our readers an overview of these devices--their value within the hospital to both patients and staff; the currently available models; and our recommendations for the best use of each model. Because the devices differ from one another in both design and application; we did not apply our typical rating system in evaluating them. We performed patient transfers using four patient transfer systems from four manufacturers; comparing them with transf | 2634657 | [
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Patient transfer systems. | ers made with a drawsheet; and assessed their ease of transfer; patient comfort; portability; setup time; and ease of cleaning. Compared with the drawsheet; all of the devices reduce the effort required for patient transfer. Most hospitals will find that no single device meets all of their needs and that a combination of several is appropriate. We welcome reader responses about this evaluation format. | 2634657 | [
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DeVilbiss Pulmo-Aide air compressors. | nan | 2634653 | [
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Evaluation test samples--evidence of an open dialog. | nan | 2634656 | [
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Allosteric transition in hemoglobin (alpha 2A beta 2I) from the rhynchocephalian reptile relict Sphenodon punctatus. | The major hemoglobin component Hb A of the tuatara; Sphenodon punctatus; a relict of the rhynochocephalian reptiles that lived 200 million years ago; was investigated in the light of the apparent contradiction inherent in an effect of organic phosphate cofactors on the oxygen affinity of hemoglobins exhibiting hyperbolic oxygen equilibrium curves. The heterotropic allosteric effect of ATP; the major erythrocytic cofactor in the tuatara; is shown to be correlated with distinct homotropic interactions (Hill's | 2634664 | [
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Allosteric transition in hemoglobin (alpha 2A beta 2I) from the rhynchocephalian reptile relict Sphenodon punctatus. | cooperativity coefficient at half-saturation; n50; attaining 1.3-1.5 above pH 7.5); and with free energies of heme-heme interaction (4.7 kJ.mole-1 at pH 7.2) which resemble those in other vertebrate hemoglobins that exhibit higher n50 values. Curiously; chloride ions increase hemoglobin-oxygen affinity below pH +/- 7.2. The possible mechanisms underlying the effects of ATP and chloride are discussed. | 2634664 | [
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Hb Miyano or alpha 41(C6)Thr----Ser: a new high oxygen affinity alpha chain variant found in an erythremic blood donor. | An abnormal hemoglobin found in an erythremic blood donor was separable only by isoelectrofocusing; where it was located at the cathodic edge of Hb A. Cation exchange high performance liquid chromatography of a tryptic digest from the total alpha chain revealed splitting of the alpha T-6 peak; although our routine procedures failed to uncover the abnormality. This enabled the chemical characterization and quantitation of the abnormal hemoglobin as alpha 41(C6)Thr----Ser comprising about 30% of the total hem | 2634665 | [
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Hb Miyano or alpha 41(C6)Thr----Ser: a new high oxygen affinity alpha chain variant found in an erythremic blood donor. | oglobin. The purified hemoglobin showed increased oxygen affinity; decreased subunit cooperativity and effect of organic phosphates; and normal Bohr effect. | 2634665 | [
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The prevalence of avascular necrosis in sickle cell anemia: correlation with alpha-thalassemia. | The purpose of the present study was to investigate the effect of alpha-thalassemia on the prevalence of avascular necrosis in 52 adult patients with sickle cell anemia. alpha-Globin genotypes were determined by restriction endonuclease mapping of genomic DNA extracted from peripheral blood leukocytes. Radiographs of humeral and femoral heads were interpreted by two radiologists who were not aware of the clinical picture and the genotype of the patients in the study. We present data showing that there is a | 2634666 | [
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The prevalence of avascular necrosis in sickle cell anemia: correlation with alpha-thalassemia. | significant positive correlation between alpha-gene deletion and the prevalence and extent of avascular necrosis in our patient population. | 2634666 | [
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The types of hemoglobins and globin chains in hydrops fetalis. | Details are presented of analyses of hemoglobins in blood samples from four newborn babies with hydrops fetalis using reversed phase and anion exchange high performance liquid chromatographic methodology. Three were homozygous for the alpha-thalassemia-1 (SEA) deletion; and one was a compound heterozygote for the same deletion and the larger alpha-thalassemia-1 (Fil) deletion. All four babies had beta; G gamma; A gamma; and zeta chains; these chains were present in Hb Bart's or gamma 4; Hb Portland-I (zeta | 2634668 | [
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The types of hemoglobins and globin chains in hydrops fetalis. | 2 gamma 2); and Hb Portland-II (zeta 2 beta 2). Hb H (beta 4) could not be detected. The level of zeta was directly related to the level of beta and; thus; the fetal age. A lower level of zeta chain was present in the baby with the compound heterozygosity because the large deletion (Fil) on one chromosome included the zeta and psi zeta genes. Circulating red cells; i.e. reticulocytes and nucleated red cells; were unable to synthesize zeta chains; indicating that this capability must have ceased a few months | 2634668 | [
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The types of hemoglobins and globin chains in hydrops fetalis. | prior to birth. Quantitative data obtained by chromatographic procedures were greatly influenced by the condition of the blood sample and the way it was stored. Hb Portland-II (zeta 2 beta 2) and Hb Bart's (gamma 4) are rather unstable when a red cell lysate is stored at 4 degrees C; this is in contrast to Hb Portland-I (zeta 2 gamma 2) which appears to be stable. Samples can be stored as washed red cells or red cell lysates at -70 degrees C. | 2634668 | [
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Characterization of beta-thalassemia mutations among the Japanese. | Characterization of beta-thalassemia mutations were attempted for 29 Japanese families clinically diagnosed as having beta-thalassemia. Following the identification of a mutation by cloning and sequencing; all families were screened for this particular mutation; using biotinylated allele-specific oligonucleotide probes. Seven different mutations were detected in 17 families: Six families had the frameshift mutation at codons 41/42; resulting from a 4 nucleotide deletion (TTCTTT----TT); four had the deletion | 2634667 | [
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Characterization of beta-thalassemia mutations among the Japanese. | at codons 127/128 (CAGGCT----CCT); and three had the TATA box mutation at nucleotide -31 (A----G). Four additional families had mutations at codon 24 (GGT----GGA); codon 26 (GAG----AAG); IVS-II-654 (C----T) and codon 110 (GTG----CCG); respectively. The newly discovered deletion mutation at codons 127/128; and mutations at nucleotide -31; and at codon 110 are peculiar to Japanese; and have not been found in any other ethnic group. The haplotypes of the beta-globin gene cluster were also determined. Some of | 2634667 | [
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] |
Characterization of beta-thalassemia mutations among the Japanese. | the haplotypes and beta-thalassemia mutations are identical to those reported in the Chinese population. However; it is noteworthy that nearly half of the beta-thalassemia mutations were unique to Japanese. | 2634667 | [
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Hb Reims [alpha 2(23)(B4)Glu----Gly beta 2]: a new alpha chain variant with slightly decreased stability. | nan | 2634669 | [
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Training rehabilitation technicians in the Pacific Basin. | A description of a unique programme designed to train and support hospital-based rehabilitation generalists on remote Pacific islands is presented. The rationale for the training is discussed; the curriculum is described and the outcomes thus far are presented. The programme consists mainly of on-site training of indigenous health personnel centred around mobility aspects of physical rehabilitation. The project has been successful in enhancing local capabilities to deal with the needs of acutely and chronic | 2634675 | [
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Training rehabilitation technicians in the Pacific Basin. | ally disabled persons and reducing the need for off-island referrals. | 2634675 | [
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Shape of the craniofacial complex in 45,X females: cephalometric study. | The shape of the craniofacial complex was analysed cephalometrically in sixty-four adult 45;X females (Turner syndrome) using lateral skull radiographs; and the subjects were compared with first-degree female relatives and control females. The results showed that 45;X females have marked changes in relatively few craniofacial areas compared to the controls. Most of the changes are located in the cranial base; so that the face is retrognathic. The mandible is short; whereas the maxilla is of normal length. T | 2634680 | [
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Shape of the craniofacial complex in 45,X females: cephalometric study. | he results support the view that the morphology of the cranial base is markedly affected in 45;X females; whereas most other craniofacial changes could be considered secondary to the cranial base abnormality. It is suggested that retarded cartilage growth may be a factor leading to the present findings. | 2634680 | [
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Epithelial bridging of the primary palate: I. Characterization of sub-cultured epithelial cells. | Primary palatogenesis involves an intricate array of events. Cell migration; proliferation; differentiation; programmed death; and fusion occur. Prior to fusion; the morphology of the epithelium undergoes marked changes. Epithelial projections form and extend across the fusion site attaching by filopodia to the opposite prominence. By appearance; the epithelium plays a critical role in facial development. In order to monitor epithelial activities; a study was done to isolate and characterize epithelial cell | 2634681 | [
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Epithelial bridging of the primary palate: I. Characterization of sub-cultured epithelial cells. | s derived from the primary palate. The primary palate was microdissected from day 13 Sprague-Dawley rat embryos; and the epithelium and mesenchyme were separated by enzymatic digestion with a 3% trypsin-pancreatin solution (3:1). All explants were cultured in Dulbecco's modified Eagle's medium (DMEM) and Ham's F-12 medium (1:1) supplemented with 10% fetal calf serum (FCS); 20 ng/ml epidermal growth factor (EGF); and antibiotics. Explant cells were gathered by trypsin harvesting and sub-cultured. These sub-c | 2634681 | [
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Epithelial bridging of the primary palate: I. Characterization of sub-cultured epithelial cells. | ultured cells were further characterized. Transmission and scanning electron microscopy showed that the cells retained many morphological features observed in vivo. In passaged cells; type IV collagen; laminin; and cytokeratins were visualized by immunocytochemistry. Gel electrophoresis analysis of the water-insoluble extracts demonstrated major bands of proteins of 50 kD and 44 kD that were synthesized by the epithelial cells but not by the mesenchymal cells. These cytokeratin types are suggestive of a sim | 2634681 | [
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Epithelial bridging of the primary palate: I. Characterization of sub-cultured epithelial cells. | ple undifferentiated embryonic epithelium. The effect of all-trans retinoic acid (RA) on cell number and [3H]-proline incorporation was assessed. At [10(-4)M] and [10(-6)M] retinoic acid resulted in significant inhibition in cell proliferation and amount of proline incorporated; with the greater inhibition occurring in the mesenchymal cells. In the concentrations studied; retinoic acid has an inhibitory effect on the two differently derived cell types. This study established that sub-cultured epithelial cel | 2634681 | [
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Epithelial bridging of the primary palate: I. Characterization of sub-cultured epithelial cells. | ls maintain their phenotype and can be used to study fusion processes. Part 2 will demonstrate how the morphology of the epithelial cells can be modified to produce the changes that are observed during fusion of the primary palate. | 2634681 | [
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Epithelial bridging of the primary palate: II. In vitro model mimics in vivo behavior. | Previously; Forbes et al. [J Craniofac Genet Dev. Biol; 9:271-284; 1989] and Millicovsky et al. [Am J Anat 164:29-44; 1982]; demonstrated that some of the epithelial cells of the primary palate formed extensive projections; bridging the medial and lateral nasal prominences. These connections are thought to aide in the fusion process by facilitating union of the prominences; a process known as secondary fusion [Millicovsky et al.; 1982]. In order to study the epithelial cell and its behavior more closely an | 2634682 | [
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Epithelial bridging of the primary palate: II. In vitro model mimics in vivo behavior. | in vitro model was established [Gibson et al.: J Craniofac Genet Dev Biol; 1989]; where epithelial cells in culture were shown to produce many of the morphologic characteristics observed in vivo. In the present study; an in vitro model is discussed which reproduces the epithelial projections observed in vivo. Epithelial cells; previously characterized; were obtained from the primary palate of 13-day-old rat embryos and sub-cultured as explants. Comparisons were made with the epithelial bridging observed in | 2634682 | [
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Epithelial bridging of the primary palate: II. In vitro model mimics in vivo behavior. | vivo of two species of animals. The results indicated sub-cultured epithelium as isolated cells; at either low or high density; rarely formed bridges. Primary cultures of epithelial explants also infrequently formed projections. However; sub-cultures of epithelial explants; plated as small clusters of cells with intervening spaces between cell groups; demonstrated extensive epithelial bridging. Epithelial projections did not form from cells that were directly attached to the plastic culture dish; only super | 2634682 | [
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Epithelial bridging of the primary palate: II. In vitro model mimics in vivo behavior. | ficial; elevated cells formed projections. Significantly; the connections that occurred between explants did not attach to the plastic substratum. Instead; they appeared as line connections suspended by the medium. With time; the number of projections increased and epithelial cells could be seen along the projections forming an epithelial bridge. This study established a model of epithelial bridging in vitro for analysis of a process which has been shown to be an integral part of primary palate fusion. | 2634682 | [
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Effects of cyclophosphamide on the secondary palate development in golden Syrian hamster: teratology, morphology, and morphometry. | Cyclophosphamide (CP); when injected in hamster mother between days 9 and 11 of pregnancy; was teratogenic in fetuses. On the basis of a morphological study it was deduced that CP delayed the reorientation of hamster palatal shelves by 16-20 h. In a subsequent experiment; in both control and CP-treated palatal shelves; the numbers of epithelial and mesenchymal cells were counted and cross-sectional area was measured. DNA synthesis; measured by 3H-thymidine incorporation; was used as an index of growth by ce | 2634683 | [
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Effects of cyclophosphamide on the secondary palate development in golden Syrian hamster: teratology, morphology, and morphometry. | ll proliferation. The results showed that during the vertical development of palatal shelves; the mesenchymal cells reached their peak number during the initial 24 hours; i.e.; at the end of the second peak in DNA synthesis; and remained unchanged thereafter throughout reorientation. The shelf area also showed rapid increase during the initial 24 h followed by a spurt 2 h prior to reorientation. Cyclophosphamide prolonged the acquisition of these features by affecting the mesenchymal cells and consequently | 2634683 | [
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Effects of cyclophosphamide on the secondary palate development in golden Syrian hamster: teratology, morphology, and morphometry. | delayed the reorientation of the vertical shelves until such time that the number of healthy mesenchymal cells and shelf area were restored to the control values. The data lend further support to the hypothesis that the acquisition of a specific number of cells and shelf volume; during vertical palatal development; may be essential for palatal shelf reorientation. | 2634683 | [
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Employment chances of recently visually impaired, young or middle-aged adults in the Swedish labour market. | The study (n = 261) focused on employment chances of Swedish visually impaired persons aged 25-45 years at the onset of their visual impairment; and who were employed at that time. It concerned persons who became visually impaired after 1974; the year when the Act on Security of Employment was passed. Although the Act has had an impact on employment opportunities; it is; however; no guarantee per se. Factors in the work environment accounted for improved chances of keeping the pre-disability employment. Ind | 2634678 | [
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Employment chances of recently visually impaired, young or middle-aged adults in the Swedish labour market. | ividual factors were decisive in a person's chances of obtaining new employment. The least competitive groups of visually impaired were; in spite of the law; 'eliminated' from the labour market. | 2634678 | [
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'Breathtaking': the consequences of chronic respiratory disorder. | Chronic obstructive airways disease (COAD) is a major; though neglected; medical and social problem in the United Kingdom today. Dyspnoea is one of the most distressing and disabling symptoms of COAD; which is itself the largest single cause of absence from work in the United Kingdom. This paper reports on 92 patients suffering from COAD; who were interviewed in order to assess impairment; disability and handicap; and a smaller subsample of 24 of these patients who were followed-up using open-ended; semi-st | 2634677 | [
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