Trypan Blue
Trypan Blue is a selective tissue staining agent (dye) which stains anterior lens capsule of the human crystalline lens. The dye does not penetrate the capsule, permitting visualization of the anterior capsule in contrast to the non-stained lens cortex and inner lens material.
The first step to administer Trypan Blue is to inject an air bubble into the anterior chamber using a 26 gauge needle. It prevents dilution of dye by the aqueous humor. Then Trypan Blue is injected into the anterior lens capsule using a tuberculin syringe. Sufficient staining is achieved as soon as the dye has contracted the capsule. If some area is left unstained, inject one more drop till it gets stained.
Trypan Blue is contraindicated when a non-hydrated (dry state), hydrophilic acrylic intraocular lens (IOL) is planned to be inserted into the eye because the dye may be absorbed by the IOL and stain the IOL.
Discoloration of high water content hydrogen intraocular lenses.Inadvertent staining of the posterior lens capsule and vitreous face.It is generally self limited, lasting up to one week.
PregnancyCategory C. There are no adequate and well-controlled studies in pregnant women. Trypan Blue should be given to a pregnant woman only if the potential benefit justifies the potential risk to the fetus.Lactation: It is not known whether this drug is excreted in human milk. Because many drugs are excreted in human milk, caution should be exercised when Trypan Blue is administered to a nursing woman.
It is recommended that after injection all excess TyBlue should be immediately removed from the eye by thorough irrigation of the anterior chamber.
Preparations for Ophthalmic diagnosis
Store below 30° C and keep away from light.
Pediatric use:The safety and effectiveness of TyBlue have been established in pediatric patients. Use of TyBlue is supported by evidence from an adequate and well controlled study in pediatric patients.Geriatric use:No overall differences in safety and effectiveness have been observed between elderly and younger patients.
{'Indications': 'TyBlue is indicated for use as an aid in ophthalmic surgery by staining the anterior capsule of the lens.'}