In Vivo and In Vitro Effects of Chlorhexidine Pretreatment on Immediate and Aged Dentin Bond Strengths. Adhesive performance of dentin bonding agents applied in vivo and in vitro. Effect of intrapulpal pressure and dentin depth. Bond strength of self-etching primer and total-etch adhesive systems to primary dentin. Bonding Performance of a Multimode Adhesive to Artificially-induced Caries-affected Primary Dentin. Experimental and clinical evaluation of a self-etching and an etch-and-rinse adhesive system. Influence of endodontic irrigants on bond strength of a self-etching adhesive. Microtensile bond strength of a total-etch 3-step, total-etch 2-step, self-etch 2-step, and a self-etch 1-step dentin bonding system through 15-month water storage. Effect of ferric sulfate contamination on the bonding effectiveness of etch-and-rinse and self-etch adhesives to superficial dentin Bonding strength to two different surfaces of dentin under simulated pulpal pressure. Shear bond strength of self-etching and total-etch adhesive systems to Er:YAG laser-irradiated primary dentin. Primary dentin etching time, bond strength and ultra-structure characterization of dentin surfaces. Bond strength of two adhesive systems to primary and permanent enamel. Effect of haemostatic agent application on the shear bond strength of contemporary/multi-mode adhesive systems. In vivo outcomes of indirect pulp treatment using a self-etching primer versus calcium hydroxide over the demineralized dentin in primary molars. Microtensile bond strength of a total-etching versus self-etching adhesive to caries-affected and intact dentin in primary teeth. Clinical performance of a self-etching and a total-etch adhesive system - 2-year results. Effect of silver diamine fluoride on microtensile bond strength to dentin.
This study evaluated the effect of 2 % chlorhexidine ( CHX ) pretreatment of dentin on the immediate and aged microtensile bond strength ( μTBS ) of different adhesives to dentin in vivo and in vitro . Class I cavities were prepared in 80 caries-free human third molars of 40 patients in a split-mouth fashion . In each tooth pair , one tooth received 2 % CHX pretreatment after which both teeth were r and omly assigned to one of the following groups with respect to the type of adhesive system applied : Adper Single Bond 2 ( etch- and -rinse ) , Clearfil SE Bond ( two-step self-etch ) , Clearfil S(3 ) Bond ( one-step self-etch ) , and Adper Prompt-L-Pop ( all-in-one self-etch ) . The teeth were restored with resin composite and extracted for μTBS testing either immediately or after six months in function . In vitro specimen pairs were prepared as with the clinical protocol in intact , freshly extracted human molars , and thereafter , subjected to testing immediately or after 5000 × thermocycling . Data were analyzed with four-way analysis of variance ( ANOVA ) . Bonferroni test was utilized for pair-wise comparisons . The immediate bond strength values were significantly higher than " aged " ones for all tested adhesives ( p=0.00 ) . The in vitro immediate bond strength values were statistically higher than in vivo bond strength values ( p<0.05 ) . While the bond strength of in vitro aged , CHX-treated sample s were higher than their in vivo counterparts ( p<0.05 ) , no difference was observed in non-CHX treated groups ( p>0.05 ) . In the absence of CHX pretreatment , all adhesives showed significantly higher immediate bond strength values than CHX-treated groups , while all " aged " , non-pretreated adhesives exhibited significantly lower bond strength values ( both p<0.05 ) . By contrast , chlorhexidine pretreatment result ed in significantly higher aged bond strengths , regardless of the adhesive system and testing condition . Aging-associated decline in dentin bond strength of etch- and rinse and self-etch adhesives can be counteracted by chlorhexidine application The purpose of this study was to evaluate the influence of intrapulpal pressure and dentin depth on bond strengths of an etch- and -rinse and a self-etching bonding agent to dentin in vitro and in vivo . Twenty-four pairs of premolars were r and omly divided into four groups ( n = 6 ) according to the dentin bonding agent , Single Bond and Clearfil SE Bond , and intrapulpal pressure , null or positive . Each tooth of the pair was further design ated to be treated in vivo or in vitro . The intrapulpal pressure was controlled in vivo by the delivery of local anesthetics containing or not a vasoconstrictor , while in vitro , it was achieved by keeping the teeth under hydrostatic pressure . Class I cavities were prepared and the dentin bonding agents were applied followed by incremental resin restoration . For the teeth treated in vitro , the same restorative procedures were performed after a 6 month-storage period . Beams with 1 mm(2 ) cross-sectional area were prepared and microtensile tested . Clearfil SE Bond was not influenced by any of the variables of the study , while bond strengths produced in vitro were significantly higher for Single Bond . Overall , lower bond strengths were produced in deep dentin , which reached statistical significance when Single Bond was applied under physiological or simulated intrapulpal pressure . In conclusion , in vitro bonding may overestimate the immediate adhesive performance of more technique-sensitive dentin bonding systems . The impact of intrapulpal pressure on bond strength seems to be more adhesive dependent than dentin morphological characteristics related to depth PURPOSE The objective of this study was to assess in vitro the tensile bond strength of a self-etching and 2 total-etch , single-bottle adhesive systems to primary dentin . METHODS Thirty-six sound primary canine buccal surfaces were r and omly assigned to 3 groups ( N = 12 ) , corresponding to the tested adhesive systems : ( 1 ) group I = Excite ( EX ) ; ( 2 ) group II = Single Bond ( SB ) ; and ( 3 ) group III = Prompt L-Pop ( PLP ) . After 24-hour storage in distilled water , tensile bond strength was tested for failure at a crosshead speed of 0.5 mm/min . RESULTS Means ( MPa ) and st and ard deviation ( + /- ) were : ( 1 ) EX = 12.72 ( + /- .89 ) ; ( 2 ) SB = 10.86 ( + /- 2.09 ) ; ( 3 ) PLP = 8.66 ( + /- 2.23 ) . Single Bond and Excite showed statistically similar results ( P > .05 ) and provided the highest means . Prompt L-Pop was statistically different from the other groups ( P < .05 ) and reached the lower bond strength to primary dentin . CONCLUSIONS Total-etch agents provided the best overall bonding performance , whereas the all-in-one , self-etching , self-priming adhesive system yielded remarkably lower bond strength to primary teeth dentin PURPOSE To investigate the bonding of a new universal adhesive applied using different etching strategies on sound and caries-affected dentin of primary teeth . MATERIAL S AND METHODS Flat dentin surfaces from 50 primary molars were r and omly assigned to 10 groups according to substrate ( sound dentin [ SD ] vs caries-affected dentin [ CAD ] pH cycled for 14 days ) and bonding approach ( Scotchbond Universal Adhesive : self-etching , vs dry or wet-bonding etch- and -rinse strategies ; Adper Single Bond Plus [ two-step etch- and -rinse adhesive ] and Clearfil SE Bond [ two-step self-etching system ] as controls ) . After 24 h of water storage , bonded sticks with cross-sectional areas of 0.8 mm2 were tested for microtensile bond strength ( μTBS ) . Two sticks from each tooth were immersed in silver nitrate solution in order to evaluate nanoleakage ( NL ) with SEM . The μTBS means were analyzed using two-way ANOVA and Tukey 's tests . For NL , the Kruskal-Wallis and Mann-Whitney tests were used ( α = 0.05 ) . RESULTS The influence of the etching strategy on the bonding performance of the universal adhesive was substrate dependent . The self-etching approach result ed in lower μTBS values and higher silver nitrate uptake into hybrid layers for Scotchbond Universal Adhesive on SD , while no difference among experimental groups was observed in CAD . CONCLUSION It is preferable to use the universal adhesive following either a dry- or wet-bonding etch- and -rinse approach on both sound and caries-affected primary dentin PURPOSE To assess the one-step self-etching adhesive iBond Gluma inside ( 1-SE ) and the two-step etch- and rinse adhesive Gluma Comfort Bond ( 2-ER ) , both used in combination with the fine particle hybrid composite Venus , in a laboratory and in a prospect i ve clinical study . MATERIAL S AND METHODS In the in vitro study , tooth-composite interaction , microleakage , and adhesive failure of 8 Class V restorations per system were evaluated by light and scanning electron microscopy . In the in vivo study , 90 paired Class III/IV restorations were scored over 48 months ( modified USPHS criteria ) . Cumulative failure rates ( CFR ) were calculated . RESULTS In vitro , microleakage and adhesive failure at enamel margins were significantly increased with 1-SE in comparison with 2-ER . With 1-SE , micromechanical interlocking at enamel was reduced and pores within the adhesive layer arose . In vivo , after 48 months with 1-SE , decreased marginal integrity and an increased number of dark marginal color lines were observed . CONCLUSION Because the results of the 4-year clinical study showed lower esthetics and marginal integrity for the 1-SE adhesive , its use should be viewed critically , which is in line with the reduced enamel-adhesive-composite interaction found in vitro The aim of this in vitro study was to evaluate the bond strength of a self-etching adhesive system to dentine irrigated with sodium hypochlorite ( NaOCl ) , chlorhexidine solution ( CX ) and ethylene diamine tetra acetic acid ( EDTA ) by microtensile testing . Sixty human third molars were sectioned 3 mm below the occlusal level and then r and omly divided into six groups : G1(control ) , without irrigant solution ; G2 , 1 % NaOCl ; G3 , 1 % NaOCl followed by the application of 17 % EDTA ; G4 , 2 % CX ; G5 , 2 % CX followed by the application of 17 % EDTA and G6 , 17 % EDTA . The specimens received the self-etching adhesive system were restored with composite resin , then sectioned and trimmed to obtain four hourglass-shaped slabs from each tooth . The specimens were su bmi tted to the microtensile test in a Universal Testing Machine at a crosshead speed of 0.5 mm min(-1 ) until fracture . The results were su bmi tted to statistical analysis by anova /Newman-Keuls . The means and st and ard deviations ( MPa ) were : G1 , 26.88 ( ±3.81 ) ; G2 , 19.08 ( ±3.89 ) ; G3 , 18.16 ( ±2.21 ) ; G4 , 18.14 ( ±4.32 ) ; G5 , 34.30 ( ±3.32 ) ; G6 , 13.61 ( ±1.21 ) . It was concluded that the application of 2 % CX followed by the application of 17 % EDTA result ed in increasing the bond strength of the self-etching adhesive system to dentine , when compared with the results obtained for the other tested groups PURPOSE To evaluate dentin bond durability using current dentin adhesive resin bonding approaches over a 15-month period of water storage . MATERIAL S AND METHODS Forty-four extracted human molars were polished with 600-grit SiC papers exposing occlusal dentin , and r and omly distributed into four adhesive groups : total-etch 3-step ( TE3 ) ( Scotchbond Multi- Purpose , 3 M ESPE ) , total-etch 2-step ( TE2 ) ( Single Bond , 3 M ESPE ) , self-etch 2-step ( SE2 ) ( Clearfil SE Bond , Kuraray ) , and a self-etch 1-step ( SE1 ) ( Prompt L-Pop , 3 M ESPE ) . A resin composite crown was incrementally formed and light cured to approximately 6 mm in height . Microtensile specimens were fabricated and stored in distilled water containing 0.5 % chloramine T and tensile tested at 1 mm/min after 1 , 6 , and 15 months . The debond pathway was recorded as either involving the substrate or joint using scanning electron microscopy . SAS software was used to compute Weibull parameters and distributions , Log-rank and Wilcoxon tests were used for comparison of survival curves over time for each adhesive system and between adhesive systems . RESULTS The TE2 was significantly weaker than TE3 and SE2 after 1 and 6 months of storage , but all three systems were equivalent after 15 months of storage . The SE1 system could not be tested due to 58 of 65 specimens failing during specimen preparation . Failure modes were observed to be dependent upon adhesive system , with only the total-etch 2-step system demonstrating an increasing involvement in the adhesive joint over time . CONCLUSION Although differences in bond strength were observed across adhesive systems up to 6 months of storage , no differences were noted at 15 months . This may represent common degradative mechanisms Aim : This study investigated the effect of one hemostatic agent on the shear bond strength of  self-etch and etch- and -rinse adhesive systems . Material s and Methods : Sixty extracted third molars were selected . After preparing a flat surface of superficial dentin , they were r and omly divided into six groups . Adhesives were Tetric N-Bond , AdheSE , and AdheSE One F. Before applying adhesives , surfaces were contaminated with ViscoStat for 60 s in three groups and rinsed . Then composite were attached to surfaces and light cured . After thermocycling , the bond strength was calculated and failure modes were determined by stereomicroscope . The data were analyzed by t-test and one-way ANOVA with P < 0.05 as the level of significance . Results : ViscoStat had significantly decreased the shear bond strength of AdheSE ( P < 0.0001 ) to dentin . Modes of failures in all groups were mainly adhesive . Conclusion : Contamination had an adverse effect on the shear bond strength of AdheSE and reduced it PURPOSE The purpose of this in vitro study was to measure and compare the in vitro shear bond strengths ( SBS ) of the following dentin bonding systems to different dentin surfaces under simulated pulpal pressure : Liner Bond 2 ( self-etching ) ( LB2 ) , Liner Bond 2V ( self-etching ) ( LB2V ) , Opti Bond Solo ( single component ) ( OBS ) , Fuji Bond LC ( filled ) ( FBLC ) , Prime & Bond 2 - 1 ( single component ) ( P&B2 - 1 ) , and Solid Bond ( total etching ) ( SB ) . MATERIAL S AND METHODS Occlusal and buccal surfaces of 60 extracted human molars were prepared to expose the dentin bonding surface . After being wet ground with 600-grit abrasive papers , teeth were r and omly assigned to 6 equal groups of 10 each . The dentin surfaces were treated with the bonding systems mentioned above , and resin composite cylinders ( Clearfil AP-X ) were built up under simulated pulpal pressure when diluted bovine serum was used as the pulpal fluid . After one day of storage in 37 degrees C water , the specimens were shear tested to failure on an Instron machine . RESULTS The data were statistically analyzed with the Student 's t-test and a post hoc Tukey test . Comparing buccal surfaces , the post hoc Tukey test indicated no significant differences among all the test groups ( p > 0.05 ) . On occlusal surfaces , the post hoc Tukey test indicated significant differences among the tested groups ( p < 0.05 ) . The Student 's t test revealed significant differences between the occlusal and buccal shear bond strength values in LB 2 , LB2V , and SB groups ( p < 0.05 ) . CONCLUSION The strength of adhesion to dentin depends upon both the adhesive system used - with self-etching systems tending to show higher bond strengths than the other material s tested - and the location of the dentin , with significantly higher SBS being found on buccal surfaces in 3 of the 7 tested bonding systems PURPOSE The purpose of this study was to assess in vitro the shear bond strength of self-etching and total-etch adhesive systems to Er : YAG laser-irradiated primary dentin . METHODS Forty crowns of primary canines were embedded in acrylic resin and mechanically ground to expose a flat dentin surface . The specimens were r and omly assigned to 2 groups ( N=20 ) , according to the adhesive system : ( A ) Single Bond ( SB ) ; and ( B ) Adper Prompt ( AP ) . Each group was divided into 2 subgroups ( N=10 ) , depending on the surface treatment : ( 1 ) conventional bonding protocol , as recommended by the manufacturers ; and ( 2 ) irradiation of the dentin site with a 2.94-microm wavelength Er : YAG laser , with a 300-mJ pulse energy and a 2-Hz repetition rate followed by the bonding protocol . In both groups , a 3-mm diameter dentin bonding site was demarcated , the adhesive systems were applied , and resin composite cylinders were bonded . After 24 hours in distilled water , shear bond strength was tested at a crosshead speed of 0.5 mm/minute . RESULTS Means ( in MPa ) were : group A1=14.14(+/-1.7 ) ; group A2=8.41(+/-1.04 ) ; group B1=6.88(+/-1.12 ) ; and group B2=4.19(+/-0.7 ) . Data were su bmi tted to statistical analysis using 2-way analysis of variance and t test at 5 % significance level . CONCLUSION Irradiation of primary dentin with the Er : YAG laser decreased the bond strength of total-etch and self-etching adhesive systems OBJECTIVES To evaluate the effect of shortening the etching time on roughness , microhardness and bond strength of three adhesive systems to primary tooth dentin . METHODS Flat dentin surfaces from primary molars were r and omly assigned to six experimental groups . Three different adhesive systems were used : an etch- and -rinse adhesive ( Single Bond ) , a two-step self-etching ( Clearfil SE Bond ) , and a one-step self-etching ( One-Up Bond F ) adhesive . In half of the specimens , the recommended etching time was used , in the other half the etching time was 50 % reduced . After applying the adhesive , resin composite build-ups were constructed and stored in a humid environment for 24h at 37 degrees C. Specimens were sectioned into 1mm(2 ) beams and tested for microtensile bond strength ( MTBS ) . Debonded surfaces were analyzed by scanning electron microscopy ( SEM ) . Additional surfaces were conditioned for microhardness measurements ( KHN ) and for atomic force microscopy ( AFM ) analysis . Intertubular and total surface roughness ( Ra ) were recorded . Results were analyzed with ANOVA and Student-Newman-Keuls tests ( P<0.05 ) . RESULTS Single Bond and Clearfil SE Bond showed higher MTBS than One-Up Bond F. Bond strength and intertubular roughness increased when Single Bond and One-Up Bond F were used with a reduced etching time . For Clearfil SE Bond no differences in MTBS were detected when reducing the etching time . The application of phosphoric acid , Clearfil SE Bond primer and One-Up Bond F decreases dentin microhardness . CONCLUSIONS Shortening One-Up Bond F application time and reducing the etching time of phosphoric acid to one-half of the manufacturer 's recommended etching time when using Single Bond are recommended when bonding to primary dentin The bonding performance of current adhesive systems to primary enamel has not been thoroughly research ed . This study compared the micro-shear bond strength of two adhesive systems to primary and permanent tooth enamel . Two commercially available resin adhesives , a self-etching primer system ( Clearfil SE Bond ) and a single-bottle adhesive system ( Single Bond ) used with a total-etch wet bonding technique were tested . A micro-shear bond test was used to examine the adhesive systems on mid-coronal buccal enamel of extracted primary or permanent teeth . In addition , etched enamel surfaces and etched-bonded enamel interfaces were examined using scanning electron microscopy ( SEM ) . No statistically significant differences of shear bond strength values were found between the primary and permanent enamel or the adhesive systems used ( p>0.01 ) . The SEM observations showed that both adhesive systems etched the primary enamel deeper than the permanent enamel , suggesting that the action of acid etch seemed to be more intense on primary enamel than on permanent enamel . Bonding of the adhesive systems to primary enamel was almost identical to permanent enamel OBJECTIVES The aim was to determine the effect of blood contamination and haemostatic agent application on the shear bond strength ( SBS ) of different adhesives to dentin . MATERIAL S AND METHODS Seventy-two extracted human molars were used in this study . Teeth were mounted acrylic in moulds . Mesial and distal surfaces were removed to obtain flat dentin surfaces ( n=144 ) and grinded with a 600 grit s and paper . The sample s were r and omly divided into three main groups ( N=48 ) based on the adhesive system and application procedures . GROUP 1 : No contamination ( control ) , GROUP 2 : Blood contamination , GROUP 3 : Blood Contamination+Haemostatic agent application . Each group was further divided into four subgroups : SUBGROUP I : Single Bond 2 ( etch- and -rinse ) Subgroup II : Clearfil SE Bond ( two-step self-etch ) Subgroup III : Single Bond Universal ( multimode , etch- and -rinse ) subgroup IV : Single Bond Universal ( multimode , all-in-one self-etch ) ( n=12 ) . Resin cylinders ( Filtek Z550 ) were bonded to the dentin surfaces according to the manufacturers ' instructions . A shear load was applied to the specimens using universal testing machine at a cross-head speed of 0.5 mm/min until failure . Data were analyzed statistically ( p<0.05 ) . RESULTS Statistically significant differences were observed between no contamination ( control ) and blood + haemostatic agent contamination groups for both Single Bond 2 ( etch- and -rinse ) and Single Bond Universal ( multimode , all-in-one self-etch ) ( p<0.05 ) . When the adhesive systems were compared to each other , no statistically significant differences were found in all contamination groups and control group for the mean SBS values ( p>0.05 ) . CONCLUSION When blood contamination is inevitable two step self- etching adhesive systems may be the choice of adhesive system in terms of shear bond strength OBJECTIVE To evaluate the clinical and radiographic outcomes ( 24 months ) of indirect pulp treatment ( IPT ) in primary teeth when a self-etching primer or a calcium hydroxide layer was used over the remaining carious dentin . STUDY DESIGN Primary molar teeth with deep carious lesions without signs and symptoms of irreversible pulpitis were divided into two groups , according to the capping material : Experimental group ( 1 ) : self-etching adhesive system ( Clearfill SE Bond ) ; and Control group ( 2 ) : calcium hydroxide liner ( Dycal ) . Both groups were followed up after application of a resin restoration ( 3 M - Z250 ) . RESULTS After 2 years of clinical and radiographic follow-up , no statistical difference was found between groups ( p = 1 ) . The overall success rate reached 87 % . CONCLUSION These results demonstrate that IPT has a high clinical and radiographic performance in primary teeth and is not dependent on the capping material used over the demineralized dentin BACKGROUND The objec tives of this study were to determine microtensile bond strengths of two dentin adhesives and to compare the micromorphological structure of the resin/dentin interface in caries-affected dentin with that of intact dentin . METHODS The authors r and omly divided 40 proximal dentinal carious primary teeth and 40 noncarious anterior primary teeth into two groups ( self-etching and total-etching ) . They used a caries-detecting dye as an indicator of the need to remove the outer carious dentin . The authors restored the teeth with a hybrid resin-based composite . After 24 hours ' storage in 37 C water , specimens were sectioned and shaped to form a curved section with a cross-sectional area of 1 square millimeter , then tension was applied until they fractured . The authors prepared the resin/dentin interfaces for the two bonding systems and examined them in 10 occlusal carious and 10 noncarious teeth . STATISTICAL ANALYSIS The bond strengths for intact and caries-affected dentin within the same group were analyzed via a t test . The authors compared the remaining dentin thickness ( RDT ) and dentin hardness using analysis of variance and the least significant difference test at the .05 level of significance . RESULTS The self-etching adhesive demonstrated no statistical difference in bond strength between intact and caries-affected dentin . However , the total-etching adhesive demonstrated different bond strengths for intact and caries-affected dentin . Moreover , the RDT of specimens with intact and caries-affected dentin was not significantly different , whereas the dentin hardness of caries-affected dentin was significantly lower than that of intact dentin . The authors found a thicker hybrid layer in intact and caries-affected dentin of specimens in the total-etching group . CONCLUSION The adhesives exhibited significantly different bond strengths in intact dentin of primary teeth . However , they exhibited similar bond strengths in caries-affected dentin The purpose of this controlled prospect i ve clinical study was to compare the clinical performance of the self-etching adhesive system AdheSE and the established total-etch adhesive system Excite in classes I and II cavities for a period of 2 years . Fifty patients participated in this study . Each received two restorations , one with AdheSE , one with Excite by one calibrated , non-blinded operator . The resin composite used to restore the teeth was Tetric Ceram HB . One calibrated , blinded clinician re-evaluated the restorations at baseline , after 6 , 12 and 24 months after placement using the modified Ryge criteria . For this , vitality , post-operative sensitivity , visible marginal irregularity , marginal discolouration , secondary caries , surface texture , anatomic form and filling defect were considered . After 2 years , 67 restorations were review ed in 34 patients . None of the teeth showed signs of secondary caries . Two teeth , one of each group , had to receive endodontic treatment because of pulp inflammation . All other teeth remained vital . Slight marginal discolourations were observed in six AdheSE restorations and three Excite restorations . These restorations were scored as Beta . After 2 years , an overall clinical success rate , summing up all the Alpha and Beta scores , of 97 % was found , viewing both adhesive systems together . Statistic analysis using log-rank test showed no statistic differences in the overall survival rate between the two material s tested within the observation period . It can be concluded that both adhesive systems tested demonstrated very good clinical performance in the restoration of classes I and II cavities at the end of 2 years The aim of this in vitro study was to investigate the effect of the cariostatic and preventive agent silver diamine fluoride ( SDF ) on the microtensile bond strength of resin composite to dentin . Forty-two caries-free , extracted molars were flattened occlusally and apically using a diamond saw , and the exposed occlusal dentin was polished with a series of silicon carbide papers , all under water irrigation . The teeth were then r and omly divided into six groups of seven teeth each that were treated as follows : 1 ) Peak SE self-etch bonding agent ; 2 ) 12 % SDF + Peak SE ; 3 ) 38 % SDF + Peak SE ; 4 ) Peak LC etch- and -rinse bonding agent ; 5 ) 12 % SDF + Peak LC ; and 6 ) 38 % SDF + Peak LC . Four-millimeter buildups of Amelogen Plus were incrementally placed on all teeth ; after a 24-hour storage period in distilled water , the specimens were sectioned perpendicular to the adhesive interface to produce beams of cross-sectional surface area measuring approximately 1 mm(2 ) . The beams were placed on a microtensile testing machine , which utilized a single-speed pump motor and force gauge at 20 kgf × 0.01 second to record maximum tensile force before failure occurred . Two-way analysis of variance and post hoc Tukey tests were performed to compare the effects of the SDF  on microtensile bond strength , with statistical significance set at α = 0.05 . None of the experimental groups treated with different concentrations of SDF showed a significant difference in bond strength compared to the control groups , and there was no significant difference in bond strength between self-etch and etch- and -rinse groups . However , the effect of SDF  on self-etch bonded teeth compared to etch- and -rinse bonded teeth was statistically significant ( p=0.0363 ) , specifically at the 12 % concentration . SDF does not adversely affect the bond strength of resin composite to noncarious dentin
RESULTS No statistical significant difference in bond strength between both categories was observed in caries-affected dentin at immediate evaluation and in sound dentin after ageing .
Etch- and -rinse adhesives , however , performed better in sound enamel and dentin substrates considering immediate bond strength .
None study assessed the long-term adhesive effectiveness to sound or demineralized enamel .
Although the articles included in this meta- analysis showed high heterogeneity and high risk of bias , the in vitro literature suggests superior performance of etch- and -rinse adhesives in primary teeth in comparison with self-etch systems
AIM To systematic ally review the literature for in vitro studies that evaluated the immediate or after ageing bond strength of  etch- and -rinse and self-etch adhesive systems to enamel and dentin of primary teeth .