Electronic microscopy has been used for morphology evaluation of different materials structures. However, microscopy results may be affected by several factors. Image processing methods can be used to correct and improve the quality of these results. In this paper we propose an algorithm based on starlets to perform the segmentation of scanning electron microscopy images. An application is presented in order to locate gold nanoparticles in natural rubber membranes. In this application, our method showed accuracy greater than 85 for all test images. Results given by this method will be used in future studies, to computationally estimate the density distribution of gold nanoparticles in natural rubber samples and to predict reduction kinetics of gold nanoparticles at different time periods.
Image segmentation is often used in microscopy images that are currently segmented to obtain features such as number of cells @cite_18 , separation of overlapped particles @cite_7 and skull-stripping of mouse brain @cite_14 .
@cite_18 @cite_14 @cite_7
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Segmentation of medical imagery is a challenging problem due to the complexity of the images, as well as to the absence of models of the anatomy that fully capture the possible deformations in each structure. Brain tissue is a particularly complex structure, and its segmentation is an important step for studies in temporal change detection of morphology, as well as for 3D visualization in surgical planning. In this paper, we present a method for segmentation of brain tissue from magnetic resonance images that is a combination of three existing techniques from the Computer Vision literature: EM segmentation, binary morphology, and active contour models. Each of these techniques has been customized for the problem of brain tissue segmentation in a way that the resultant method is more robust than its components. Finally, we present the results of a parallel implementation of this method on IBM''s supercomputer Power Visualization System for a database of 20 brain scans each with 256x256x124 voxels and validate those against segmentations generated by neuroanatomy experts. Abstract The segmentation of infant brain tissue images into white matter (WM), gray matter (GM), and cerebrospinal fluid (CSF) plays an important role in studying early brain development in health and disease. In the isointense stage (approximately 6–8 months of age), WM and GM exhibit similar levels of intensity in both T1 and T2 MR images, making the tissue segmentation very challenging. Only a small number of existing methods have been designed for tissue segmentation in this isointense stage; however, they only used a single T1 or T2 images, or the combination of T1 and T2 images. In this paper, we propose to use deep convolutional neural networks (CNNs) for segmenting isointense stage brain tissues using multi-modality MR images. CNNs are a type of deep models in which trainable filters and local neighborhood pooling operations are applied alternatingly on the raw input images, resulting in a hierarchy of increasingly complex features. Specifically, we used multi-modality information from T1, T2, and fractional anisotropy (FA) images as inputs and then generated the segmentation maps as outputs. The multiple intermediate layers applied convolution, pooling, normalization, and other operations to capture the highly nonlinear mappings between inputs and outputs. We compared the performance of our approach with that of the commonly used segmentation methods on a set of manually segmented isointense stage brain images. Results showed that our proposed model significantly outperformed prior methods on infant brain tissue segmentation. In addition, our results indicated that integration of multi-modality images led to significant performance improvement. Background Automated segmentation of fluorescently-labeled cell nuclei in 3D confocal microscope images is essential to many studies involving morphological and functional analysis. A common source of segmentation error is tight clustering of nuclei. There is a compelling need to minimize these errors for constructing highly automated scoring systems. Methods A combination of two approaches is presented. First, an improved distance transform combining intensity gradients and geometric distance is used for the watershed step. Second, an explicit mathematical model for the anatomic characteristics of cell nuclei such as size and shape measures is incorporated. This model is constructed automatically from the data. Deliberate initial over-segmentation of the image data is performed, followed by statistical model-based merging. A confidence score is computed for each detected nucleus, measuring how well the nucleus fits the model. This is used in combination with the intensity gradient to control the merge decisions. Results Experimental validation on a set of rodent brain cell images showed 97 concordance with the human observer and significant improvement over prior methods. Conclusions Combining a gradient-weighted distance transform with a richer morphometric model significantly improves the accuracy of automated segmentation and FISH analysis. Cytometry Part A 56A:23–36, 2003. © 2003 Wiley-Liss, Inc. Segmenting microvascular structures is an important requirement in understanding angioadaptation by which vascular networks remodel their morphological structures. Accurate segmentation for separating microvasculature structures is important in quantifying remodeling process. In this work, we utilize a deep convolutional neural network (CNN) framework for obtaining robust segmentations of microvasculature from epifluorescence microscopy imagery of mice dura mater. Due to the inhomogeneous staining of the microvasculature, different binding properties of vessels under fluorescence dye, uneven contrast and low texture content, traditional vessel segmentation approaches obtain sub-optimal accuracy. We consider a deep CNN for the purpose keeping small vessel segments and handle the challenges posed by epifluorescence microscopy imaging modality. Experimental results on ovariectomized — ovary removed (OVX) — mice dura mater epifluorescence microscopy images show that the proposed modified CNN framework obtains an highest accuracy of 99 and better than other vessel segmentation methods. The segmentation of infant brain tissue images into white matter (WM), gray matter (GM), and cerebrospinal fluid (CSF) plays an important role in studying early brain development. In the isointense phase (approximately 6–8 months of age), WM and GM exhibit similar levels of intensity in both T1 and T2 MR images, resulting in extremely low tissue contrast and thus making the tissue segmentation very challenging. The existing methods for tissue segmentation in this isointense phase usually employ patch-based sparse labeling on single T1, T2 or fractional anisotropy (FA) modality or their simply-stacked combinations without fully exploring the multi-modality information. To address the challenge, in this paper, we propose to use fully convolutional networks (FCNs) for the segmentation of isointense phase brain MR images. Instead of simply stacking the three modalities, we train one network for each modality image, and then fuse their high-layer features together for final segmentation. Specifically, we conduct a convolution-pooling stream for multimodality information from T1, T2, and FA images separately, and then combine them in high-layer for finally generating the segmentation maps as the outputs. We compared the performance of our approach with that of the commonly used segmentation methods on a set of manually segmented isointense phase brain images. Results showed that our proposed model significantly outperformed previous methods in terms of accuracy. In addition, our results also indicated a better way of integrating multi-modality images, which leads to performance improvement. We present a new automated neuron segmentation algorithm for isotropic 3D electron microscopy data. We cast the problem into the asymmetric multiway cut framework. The latter combines boundary-based segmentation (clustering) with region-based segmentation (semantic labeling) in a single problem and objective function. This joint formulation allows us to augment local boundary evidence with higherlevel biological priors, such as membership to an axonic or dendritic neurite. Joint optimization enforces consistency between evidence and priors, leading to correct resolution of many difficult boundary configurations. We show experimentally on a FIB SEM dataset of mouse cortex that the new approach outperforms existing hierarchical segmentation and multicut algorithms which only use boundary evidence. We consider the problem of segmenting a biomedical image into anatomical regions of interest. We specifically address the frequent scenario where we have no paired training data that contains images and their manual segmentations. Instead, we employ unpaired segmentation images that we use to build an anatomical prior. Critically these segmentations can be derived from imaging data from a different dataset and imaging modality than the current task. We introduce a generative probabilistic model that employs the learned prior through a convolutional neural network to compute segmentations in an unsupervised setting. We conducted an empirical analysis of the proposed approach in the context of structural brain MRI segmentation, using a multi-study dataset of more than 14,000 scans. Our results show that an anatomical prior enables fast unsupervised segmentation which is typically not possible using standard convolutional networks. The integration of anatomical priors can facilitate CNN-based anatomical segmentation in a range of novel clinical problems, where few or no annotations are available and thus standard networks are not trainable. The code, model definitions and model weights are freely available at http: github.com adalca neuron. Abstract Accurate and reliable brain tumor segmentation is a critical component in cancer diagnosis, treatment planning, and treatment outcome evaluation. Build upon successful deep learning techniques, a novel brain tumor segmentation method is developed by integrating fully convolutional neural networks (FCNNs) and Conditional Random Fields (CRFs) in a unified framework to obtain segmentation results with appearance and spatial consistency. We train a deep learning based segmentation model using 2D image patches and image slices in following steps: 1) training FCNNs using image patches; 2) training CRFs as Recurrent Neural Networks (CRF-RNN) using image slices with parameters of FCNNs fixed; and 3) fine-tuning the FCNNs and the CRF-RNN using image slices. Particularly, we train 3 segmentation models using 2D image patches and slices obtained in axial, coronal and sagittal views respectively, and combine them to segment brain tumors using a voting based fusion strategy. Our method could segment brain images slice-by-slice, much faster than those based on image patches. We have evaluated our method based on imaging data provided by the Multimodal Brain Tumor Image Segmentation Challenge (BRATS) 2013, BRATS 2015 and BRATS 2016. The experimental results have demonstrated that our method could build a segmentation model with Flair, T1c, and T2 scans and achieve competitive performance as those built with Flair, T1, T1c, and T2 scans. The foolproof segmentation of 3D anatomical structures in medical images is usually a challenging task, which makes automatic results often far from desirable and interactive repairs necessary. In the past, we introduced a first solution to resume segmentation from third-party software into an initial optimum-path forest for interactive correction by differential image foresting transforms (DIFTs). Here, we present a new method that estimates the initial forest (input segmentation) rooted at more regularly separated seed voxels to facilitate interactive editing. The forest is a supervoxel segmentation from seeds that result from a sequence of image foresting transforms to conform as much as possible the supervoxel boundaries to the boundaries of the object in the input segmentation. We demonstrate the advantages of the new method over the previous one by using a robot user, as an impartial way to correct brain segmentation in MR-T1 images. Segmentation of 3D images is a fundamental problem in biomedical image analysis. Deep learning (DL) approaches have achieved the state-of-the-art segmentation performance. To exploit the 3D contexts using neural networks, known DL segmentation methods, including 3D convolution, 2D convolution on the planes orthogonal to 2D slices, and LSTM in multiple directions, all suffer incompatibility with the highly anisotropic dimensions in common 3D biomedical images. In this paper, we propose a new DL framework for 3D image segmentation, based on a combination of a fully convolutional network (FCN) and a recurrent neural network (RNN), which are responsible for exploiting the intra-slice and inter-slice contexts, respectively. To our best knowledge, this is the first DL framework for 3D image segmentation that explicitly leverages 3D image anisotropism. Evaluating using a dataset from the ISBI Neuronal Structure Segmentation Challenge and in-house image stacks for 3D fungus segmentation, our approach achieves promising results, comparing to the known DL-based 3D segmentation approaches. Accurate segmentation of infant brain images into different regions of interest is one of the most important fundamental steps in studying early brain development. In the isointense phase (approximately 6–8 months of age), white matter and gray matter exhibit similar levels of intensities in magnetic resonance (MR) images, due to the ongoing myelination and maturation. This results in extremely low tissue contrast and thus makes tissue segmentation very challenging. Existing methods for tissue segmentation in this isointense phase usually employ patch-based sparse labeling on single modality. To address the challenge, we propose a novel 3-D multimodal fully convolutional network (FCN) architecture for segmentation of isointense phase brain MR images. Specifically, we extend the conventional FCN architectures from 2-D to 3-D, and, rather than directly using FCN, we intuitively integrate coarse (naturally high-resolution) and dense (highly semantic) feature maps to better model tiny tissue regions, in addition, we further propose a transformation module to better connect the aggregating layers; we also propose a fusion module to better serve the fusion of feature maps. We compare the performance of our approach with several baseline and state-of-the-art methods on two sets of isointense phase brain images. The comparison results show that our proposed 3-D multimodal FCN model outperforms all previous methods by a large margin in terms of segmentation accuracy. In addition, the proposed framework also achieves faster segmentation results compared to all other methods. Our experiments further demonstrate that: 1) carefully integrating coarse and dense feature maps can considerably improve the segmentation performance; 2) batch normalization can speed up the convergence of the networks, especially when hierarchical feature aggregations occur; and 3) integrating multimodal information can further boost the segmentation performance. Segmentation of infant brain MR images is challenging due to insufficient image quality, severe partial volume effect, and the ongoing maturation and myelination processes. In particular, the image contrast inverts around 6–8 months of age, and the white and gray matter tissues are isointense in both T1- and T2-weighted MR images and thus exhibit the extremely low tissue contrast, which poses the significant challenges for automated segmentation. Most previous studies used multi-atlas label fusion strategy, which has the limitation of equally treating the available multi-modality images and is often computationally expensive. In this paper, we propose a novel learning-based multi-source integration framework for infant brain image segmentation. Specifically, we employ the random forest technique to effectively integrate features from multi-source images together for tissue segmentation. The multi-source images include initially only the multi-modality (T1, T2 and FA) images and later also the iteratively estimated and refined tissue probability maps of gray matter, white matter, and cerebrospinal fluid. Experimental results on 119 infant subjects and MICCAI challenges show that the proposed method achieves better performance than other state-of-the-art automated segmentation methods, with significantly reduction of running time from hours to 5 minutes. In the field of neuroanatomy, automatic segmentation of electron microscopy images is becoming one of the main limiting factors in getting new insights into the functional structure of the brain. We propose a novel framework for the segmentation of thin elongated structures like membranes in a neuroanatomy setting. The probability output of a random forest classifier is used in a regular cost function, which enforces gap completion via perceptual grouping constraints. The global solution is efficiently found by graph cut optimization. We demonstrate substantial qualitative and quantitative improvement over state-of the art segmentations on two considerably different stacks of ssTEM images as well as in segmentations of streets in satellite imagery. We demonstrate that the superior performance of our method yields fully automatic 3D reconstructions of dendrites from ssTEM data. The Image segmentation is referred to as one of the most important processes of image processing. Image segmentation is the technique of dividing or partitioning an image into parts, called segments. It is mostly useful for applications like image compression or object recognition, because for these types of applications, it is inefficient to process the whole image. So, image segmentation is used to segment the parts from image for further processing. There exist several image segmentation techniques, which partition the image into several parts based on certain image features like pixel intensity value, color, texture, etc. These all techniques are categorized based on the segmentation method used. In this paper the various image segmentation techniques are reviewed, discussed and finally a comparison of their advantages and disadvantages is listed. Surgical tool segmentation in endoscopic images is the first step towards pose estimation and (sub-)task automation in challenging minimally invasive surgical operations. While many approaches in the literature have shown great results using modern machine learning methods such as convolutional neural networks, the main bottleneck lies in the acquisition of a large number of manually-annotated images for efficient learning. This is especially true in surgical context, where patient-to-patient differences impede the overall generalizability. In order to cope with this lack of annotated data, we propose a self-supervised approach in a robot-assisted context. To our knowledge, the proposed approach is the first to make use of the kinematic model of the robot in order to generate training labels. The core contribution of the paper is to propose an optimization method to obtain good labels for training despite an unknown hand-eye calibration and an imprecise kinematic model. The labels can subsequently be used for fine-tuning a fully-convolutional neural network for pixel-wise classification. As a result, the tool can be segmented in the endoscopic images without needing a single manually-annotated image. Experimental results on phantom and in vivo datasets obtained using a flexible robotized endoscopy system are very promising. Image segmentation based on convolutional neural networks is proving to be a powerful and efficient solution for medical applications. However, the lack of annotated data, presence of artifacts and variability in appearance can still result in inconsistencies during the inference. We choose to take advantage of the invariant nature of anatomical structures, by enforcing a semantic constraint to improve the robustness of the segmentation. The proposed solution is applied on a brain structures segmentation task, where the output of the network is constrained to satisfy a known adjacency graph of the brain regions. This criteria is introduced during the training through an original penalization loss named NonAdjLoss. With the help of a new metric, we show that the proposed approach significantly reduces abnormalities produced during the segmentation. Additionally, we demonstrate that our framework can be used in a semi-supervised way, opening a path to better generalization to unseen data. Abstract Neonatal brain MRI segmentation is a challenging problem due to its poor image quality. Atlas-based segmentation approaches have been widely used for guiding brain tissue segmentation. Existing brain atlases are usually constructed by equally averaging pre-segmented images in a population. However, such approaches diminish local inter-subject structural variability and thus lead to lower segmentation guidance capability. To deal with this problem, we propose a multi-region-multi-reference framework for atlas-based neonatal brain segmentation. For each region of a brain parcellation, a population of spatially normalized pre-segmented images is clustered into a number of sub-populations. Each sub-population of a region represents an independent distribution from which a regional probability atlas can be generated. A selection of these regional atlases, across different sub-regions, will in the end be adaptively combined to form an overall atlas specific to the query image. Given a query image, the determination of the appropriate set of regional atlases is achieved by comparing the query image regionally with the reference, or exemplar, of each sub-population. Upon obtaining an overall atlas, an atlas-based joint registration–segmentation strategy is employed to segment the query image. Since the proposed method generates an atlas which is significant more similar to the query image than the traditional average-shape atlas, better tissue segmentation results can be expected. This is validated by applying the proposed method on a large set of neonatal brain images available in our institute. Experimental results on a randomly selected set of 10 neonatal brain images indicate that the proposed method achieves higher tissue overlap rates and lower standard deviations (SDs) in comparison with manual segmentations, i.e., 0.86 (SD 0.02) for GM, 0.83 (SD 0.03) for WM, and 0.80 (SD 0.05) for CSF. The proposed method also outperforms two other average-shape atlas-based segmentation methods. We address a central problem of neuroanatomy, namely, the automatic segmentation of neuronal structures depicted in stacks of electron microscopy (EM) images. This is necessary to efficiently map 3D brain structure and connectivity. To segment biological neuron membranes, we use a special type of deep artificial neural network as a pixel classifier. The label of each pixel (membrane or non-membrane) is predicted from raw pixel values in a square window centered on it. The input layer maps each window pixel to a neuron. It is followed by a succession of convolutional and max-pooling layers which preserve 2D information and extract features with increasing levels of abstraction. The output layer produces a calibrated probability for each class. The classifier is trained by plain gradient descent on a 512 × 512 × 30 stack with known ground truth, and tested on a stack of the same size (ground truth unknown to the authors) by the organizers of the ISBI 2012 EM Segmentation Challenge. Even without problem-specific postprocessing, our approach outperforms competing techniques by a large margin in all three considered metrics, i.e. rand error, warping error and pixel error. For pixel error, our approach is the only one outperforming a second human observer. Foreground object segmentation is a critical step for many image analysis tasks. While automated methods can produce high-quality results, their failures disappoint users in need of practical solutions. We propose a resource allocation framework for predicting how best to allocate a fixed budget of human annotation effort in order to collect higher quality segmentations for a given batch of images and automated methods. The framework is based on a proposed prediction module that estimates the quality of given algorithm-drawn segmentations. We demonstrate the value of the framework for two novel tasks related to "pulling the plug" on computer and human annotators. Specifically, we implement two systems that automatically decide, for a batch of images, when to replace 1) humans with computers to create coarse segmentations required to initialize segmentation tools and 2) computers with humans to create final, fine-grained segmentations. Experiments demonstrate the advantage of relying on a mix of human and computer efforts over relying on either resource alone for segmenting objects in three diverse datasets representing visible, phase contrast microscopy, and fluorescence microscopy images. The purpose of this study is to evaluate and compare the performance of modern deep learning techniques for automatically recognizing and segmenting multiple organ regions on 3D CT images. CT image segmentation is one of the important task in medical image analysis and is still very challenging. Deep learning approaches have demonstrated the capability of scene recognition and semantic segmentation on nature images and have been used to address segmentation problems of medical images. Although several works showed promising results of CT image segmentation by using deep learning approaches, there is no comprehensive evaluation of segmentation performance of the deep learning on segmenting multiple organs on different portions of CT scans. In this paper, we evaluated and compared the segmentation performance of two different deep learning approaches that used 2D- and 3D deep convolutional neural networks (CNN) without- and with a pre-processing step. A conventional approach that presents the state-of-the-art performance of CT image segmentation without deep learning was also used for comparison. A dataset that includes 240 CT images scanned on different portions of human bodies was used for performance evaluation. The maximum number of 17 types of organ regions in each CT scan were segmented automatically and compared to the human annotations by using ratio of intersection over union (IU) as the criterion. The experimental results demonstrated the IUs of the segmentation results had a mean value of 79 and 67 by averaging 17 types of organs that segmented by a 3D- and 2D deep CNN, respectively. All the results of the deep learning approaches showed a better accuracy and robustness than the conventional segmentation method that used probabilistic atlas and graph-cut methods. The effectiveness and the usefulness of deep learning approaches were demonstrated for solving multiple organs segmentation problem on 3D CT images.