Phenotyping is the process of measuring an organism's observable traits. Manual phenotyping of crops is a labor-intensive, time-consuming, costly, and error prone process. Accurate, automated, high-throughput phenotyping can relieve a huge burden in the crop breeding pipeline. In this paper, we propose a scalable, high-throughput approach to automatically count and segment panicles (heads), a key phenotype, from aerial sorghum crop imagery. Our counting approach uses the image density map obtained from dot or region annotation as the target with a novel deep convolutional neural network architecture. We also propose a novel instance segmentation algorithm using the estimated density map, to identify the individual panicles in the presence of occlusion. With real Sorghum aerial images, we obtain a mean absolute error (MAE) of 1.06 for counting which is better than using well-known crowd counting approaches such as CCNN, MCNN and CSRNet models. The instance segmentation model also produces respectable results which will be ultimately useful in reducing the manual annotation workload for future data.
For agronomy applications, such as ours, understanding the crop is fundamental to developing an effective algorithm. A well executed approach that illustrates this is @cite_41 that count tomatoes by first segmenting the image then a decision tree extracts the fruit segments. The decision tree used color, shape, texture and size features to locate the tomatoes. A similar approach was taken in @cite_57 for counting panicles in Sorghum. This is the only other published work we are aware of for panicle counting.
@cite_41 @cite_57
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Abstract The overall goal of this study is to develop an effective, simple, aptly computer vision algorithm to detect and count citrus on the tree using image processing techniques, to estimate the yield, and to compare the yield estimation results obtained through several methods. This new citrus recognition and counting algorithm was utilized the color features (or schemes) to present an estimate of the citrus yield, and the corresponding models are developed to provide an early estimation of the citrus yield. Citrus images were taken from Jeju, South Korea during daylight and the citrus recognition and counting algorithm were tested on 84 images which were collected from 21 trees. The citrus counting algorithm consisted of the following steps: convert RGB image to HSV, thresholding, orange color detection, noise removal, watershed segmentation, and counting. Distance transform and marker-controlled watershed algorithms were evaluated for automated watershed segmentation in citrus fruits to obtain good result. A correlation coefficient R 2 of 0.93 was obtained between the citrus counting algorithm and counting performed through human observation. The proposed algorithm showed great potential for early prediction of the yield of single citrus trees and the possibility of its uses for further fruit crops. Fully automated yield estimation of intact fruits prior to harvesting provides various benefits to farmers. Until now, several studies have been conducted to estimate fruit yield using image-processing technologies. However, most of these techniques require thresholds for features such as color, shape and size. In addition, their performance strongly depends on the thresholds used, although optimal thresholds tend to vary with images. Furthermore, most of these techniques have attempted to detect only mature and immature fruits, although the number of young fruits is more important for the prediction of long-term fluctuations in yield. In this study, we aimed to develop a method to accurately detect individual intact tomato fruits including mature, immature and young fruits on a plant using a conventional RGB digital camera in conjunction with machine learning approaches. The developed method did not require an adjustment of threshold values for fruit detection from each image because image segmentation was conducted based on classification models generated in accordance with the color, shape, texture and size of the images. The results of fruit detection in the test images showed that the developed method achieved a recall of 0.80, while the precision was 0.88. The recall values of mature, immature and young fruits were 1.00, 0.80 and 0.78, respectively. Yield mapping for tree crops by mechanical harvesting requires automatic detection and counting of fruits in tree canopy. However, partial occlusion, shape irregularity, varying illumination, multiple sizes and similarity with the background make fruit identification a very difficult task to achieve. Therefore, immature green citrus-fruit detection within a green canopy is a challenging task due to all the above-mentioned problems. A novel algorithmic technique was used to detect immature green citrus fruit in tree canopy under natural outdoor conditions. Shape analysis and texture classification were two integral parts of the algorithm. Shape analysis was conducted to detect as many fruits as possible. Texture classification by a support vector machine (SVM), Canny edge detection combined with a graph-based connected component algorithm and Hough line detection, were used to remove false positives. Next, keypoints were detected using a scale invariant feature transform (SIFT) algorithm and to further remove false positives. A majority voting scheme was implemented to make the algorithm more robust. The algorithm was able to accurately detect and count 80.4 of citrus fruit in a validation set of images acquired from a citrus grove under natural outdoor conditions. The algorithm could be further improved to provide growers early yield estimation so that growers can manage grove more efficiently on a site-specific basis to increase yield and profit. Recently, a body of computer vision research has studied the task of high-throughput plant phenotyping (measurement of plant attributes). The goal is to more rapidly and more accurately estimate plant properties as compared to conventional manual methods. In this work, we develop a method to measure two primary yield attributes of interest; stalk count and stalk width that are important for many broad-acre annual crops (sorghum, sugarcane, corn, maize for example). Prior work of using convolutional deep neural networks for plant analysis has either focused on object detection or dense image segmentation. In our work, we develop a novel pipeline that accurately extracts both detected object regions and dense semantic segmentation for extracting both stalk counts and stalk width. A ground-robot called the Robotanist is used to deploy a high-resolution stereo imager to capture dense image data of experimental plots of Sorghum plants. We ground-truth validate data extracted using two humans who assess the traits independently and we compare both accuracy and efficiency of human versus robotic measurements. Our method yields R-squared correlation of 0.88 for stalk count and a mean absolute error of 2.77 mm where average stalk width is 14.354 mm. Our approach is 30 times faster for stalk count and 270 times faster for stalk width measurement.